AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1286140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286140 MtrunA17_Chr8g0361341 70.769 65 13 1 20 84 5 63 2.24e-25 92.0
Msa1286140 MtrunA17_Chr8g0361301 70.769 65 13 1 20 84 673 731 1.08e-23 93.2
Msa1286140 MtrunA17_Chr8g0361261 70.769 65 13 1 20 84 704 762 1.13e-23 93.2
Msa1286140 MtrunA17_Chr8g0361251 69.231 65 14 1 20 84 673 731 1.23e-22 90.1
Msa1286140 MtrunA17_Chr3g0084631 60.000 65 20 1 20 84 30 88 9.26e-21 80.9
Msa1286140 MtrunA17_Chr3g0084431 60.938 64 19 1 20 83 661 718 2.28e-20 83.6
Msa1286140 MtrunA17_Chr3g0084691 60.938 64 19 1 20 83 689 746 2.61e-20 83.6
Msa1286140 MtrunA17_Chr3g0084681 60.938 64 19 1 20 83 631 688 4.20e-20 82.8
Msa1286140 MtrunA17_Chr3g0084661 60.938 64 19 1 20 83 200 257 5.65e-20 82.0
Msa1286140 MtrunA17_Chr3g0084411 60.938 64 19 1 20 83 666 723 7.84e-20 82.0
Msa1286140 MtrunA17_Chr3g0084421 59.375 64 20 1 20 83 629 686 2.40e-19 80.9
Msa1286140 MtrunA17_Chr3g0098781 59.375 64 20 1 20 83 78 135 8.12e-19 77.0
Msa1286140 MtrunA17_Chr3g0084441 57.353 68 23 1 16 83 669 730 8.85e-19 79.3
Msa1286140 MtrunA17_Chr3g0084451 55.882 68 24 1 16 83 669 730 1.93e-18 78.2
Msa1286140 MtrunA17_Chr2g0280681 63.462 52 19 0 20 71 668 719 4.87e-18 77.0
Msa1286140 MtrunA17_Chr3g0084491 59.375 64 20 1 20 83 673 730 5.11e-18 77.0
Msa1286140 MtrunA17_Chr4g0023221 60.784 51 20 0 20 70 897 947 1.60e-16 72.8
Msa1286140 MtrunA17_Chr7g0236471 57.407 54 23 0 20 73 507 560 4.63e-16 71.6
Msa1286140 MtrunA17_Chr4g0048631 60.784 51 20 0 20 70 699 749 5.27e-16 71.2
Msa1286140 MtrunA17_Chr7g0236691 57.407 54 23 0 20 73 667 720 3.59e-15 68.9
Msa1286140 MtrunA17_Chr7g0236641 57.407 54 23 0 20 73 692 745 5.96e-15 68.2
Msa1286140 MtrunA17_Chr8g0360201 62.745 51 18 1 20 70 573 622 7.40e-15 68.2
Msa1286140 MtrunA17_Chr4g0048641 36.441 118 26 2 2 70 558 675 7.42e-15 68.2
Msa1286140 MtrunA17_Chr7g0236531 55.769 52 23 0 20 71 205 256 7.65e-15 67.8
Msa1286140 MtrunA17_Chr7g0236561 53.704 54 25 0 20 73 672 725 1.02e-14 67.8
Msa1286140 MtrunA17_Chr8g0357691 53.448 58 27 0 14 71 639 696 1.11e-14 67.4
Msa1286140 MtrunA17_Chr7g0236551 55.769 52 23 0 20 71 655 706 2.24e-14 66.6
Msa1286140 MtrunA17_Chr2g0280711 59.184 49 20 0 20 68 697 745 2.33e-14 66.6
Msa1286140 MtrunA17_Chr3g0078521 60.000 50 20 0 21 70 687 736 3.06e-14 66.2
Msa1286140 MtrunA17_Chr3g0078541 60.000 50 20 0 21 70 698 747 3.06e-14 66.2
Msa1286140 MtrunA17_Chr2g0280691 62.745 51 18 1 20 70 693 742 3.09e-14 66.2
Msa1286140 MtrunA17_Chr7g0236231 55.556 54 24 0 20 73 685 738 3.68e-14 66.2
Msa1286140 MtrunA17_Chr4g0048801 53.846 52 24 0 20 71 685 736 4.18e-14 65.9
Msa1286140 MtrunA17_Chr8g0361281 54.902 51 23 0 20 70 680 730 4.52e-14 65.9
Msa1286140 MtrunA17_Chr4g0042361 54.902 51 23 0 20 70 706 756 4.61e-14 65.9
Msa1286140 MtrunA17_Chr3g0078551 58.000 50 21 0 21 70 692 741 5.08e-14 65.9
Msa1286140 MtrunA17_Chr8g0361141 60.784 51 19 1 20 70 683 732 5.18e-14 65.5
Msa1286140 MtrunA17_Chr4g0055011 50.000 72 30 1 20 91 238 303 5.20e-14 65.5
Msa1286140 MtrunA17_Chr4g0048681 59.615 52 21 0 20 71 447 498 5.94e-14 65.5
Msa1286140 MtrunA17_Chr4g0042341 56.863 51 22 0 20 70 690 740 6.61e-14 65.5
Msa1286140 MtrunA17_Chr2g0323231 56.863 51 22 0 20 70 345 395 8.55e-14 65.1
Msa1286140 MtrunA17_Chr8g0354271 59.184 49 20 0 20 68 502 550 8.65e-14 65.1
Msa1286140 MtrunA17_Chr8g0357661 51.923 52 25 0 20 71 30 81 1.41e-13 62.4
Msa1286140 MtrunA17_Chr8g0357651 55.769 52 23 0 20 71 637 688 1.66e-13 64.3
Msa1286140 MtrunA17_Chr3g0081001 59.184 49 20 0 20 68 510 558 1.68e-13 64.3
Msa1286140 MtrunA17_Chr8g0357761 54.902 51 23 0 20 70 647 697 1.89e-13 63.9
Msa1286140 MtrunA17_Chr8g0340071 44.595 74 33 1 20 85 670 743 2.03e-13 63.9
Msa1286140 MtrunA17_Chr4g0023111 56.863 51 22 0 20 70 881 931 2.19e-13 63.9
Msa1286140 MtrunA17_Chr7g0236651 53.704 54 25 0 20 73 652 705 2.34e-13 63.9
Msa1286140 MtrunA17_Chr3g0078531 58.000 50 21 0 21 70 695 744 2.77e-13 63.5
Msa1286140 MtrunA17_Chr6g0474871 56.604 53 22 1 20 71 732 784 3.31e-13 63.5
Msa1286140 MtrunA17_Chr6g0453561 58.824 51 21 0 21 71 35 85 3.51e-13 61.6
Msa1286140 MtrunA17_Chr4g0048661 57.692 52 22 0 20 71 204 255 3.54e-13 63.2
Msa1286140 MtrunA17_Chr4g0048791 57.692 52 22 0 20 71 699 750 4.05e-13 63.2
Msa1286140 MtrunA17_Chr4g0042401 50.980 51 25 0 20 70 670 720 4.21e-13 63.2
Msa1286140 MtrunA17_Chr3g0081011 59.184 49 20 0 20 68 506 554 4.72e-13 62.8
Msa1286140 MtrunA17_Chr6g0474811 54.717 53 23 1 20 71 740 792 5.83e-13 62.8
Msa1286140 MtrunA17_Chr8g0357831 53.846 52 24 0 20 71 359 410 6.89e-13 62.4
Msa1286140 MtrunA17_Chr6g0474901 50.820 61 29 1 12 71 725 785 7.08e-13 62.4
Msa1286140 MtrunA17_Chr8g0358081 51.923 52 25 0 20 71 628 679 7.32e-13 62.4
Msa1286140 MtrunA17_Chr2g0318841 58.824 51 20 1 20 70 668 717 7.62e-13 62.4
Msa1286140 MtrunA17_Chr3g0092301 57.692 52 22 0 20 71 97 148 7.88e-13 61.6
Msa1286140 MtrunA17_Chr4g0048741 55.769 52 23 0 20 71 699 750 8.50e-13 62.4
Msa1286140 MtrunA17_Chr8g0358071 52.941 51 24 0 20 70 643 693 9.81e-13 62.0
Msa1286140 MtrunA17_Chr6g0474991 50.820 61 29 1 12 71 22 82 1.01e-12 60.5
Msa1286140 MtrunA17_Chr8g0340051 56.000 50 22 0 20 69 196 245 1.08e-12 61.6
Msa1286140 MtrunA17_Chr6g0474861 56.604 53 22 1 20 71 453 505 1.17e-12 61.6
Msa1286140 MtrunA17_Chr8g0357711 53.846 52 24 0 20 71 644 695 1.22e-12 61.6
Msa1286140 MtrunA17_Chr8g0357881 52.941 51 24 0 20 70 643 693 1.24e-12 61.6
Msa1286140 MtrunA17_Chr8g0358121 51.923 52 25 0 20 71 629 680 1.28e-12 61.6
Msa1286140 MtrunA17_Chr8g0357981 52.941 51 24 0 20 70 472 522 1.28e-12 61.6
Msa1286140 MtrunA17_Chr8g0340111 56.000 50 22 0 20 69 672 721 1.30e-12 61.6
Msa1286140 MtrunA17_Chr3g0081031 58.333 48 20 0 20 67 519 566 1.33e-12 61.6
Msa1286140 MtrunA17_Chr6g0475091 54.717 53 23 1 20 71 748 800 1.44e-12 61.6
Msa1286140 MtrunA17_Chr4g0048781 55.769 52 23 0 20 71 155 206 1.45e-12 61.2
Msa1286140 MtrunA17_Chr8g0357631 53.846 52 24 0 20 71 635 686 1.52e-12 61.6
Msa1286140 MtrunA17_Chr5g0394011 58.000 50 21 0 21 70 519 568 1.61e-12 61.2
Msa1286140 MtrunA17_Chr1g0206361 52.941 51 24 0 20 70 510 560 1.61e-12 61.2
Msa1286140 MtrunA17_Chr8g0358101 51.923 52 25 0 20 71 631 682 1.63e-12 61.2
Msa1286140 MtrunA17_Chr2g0280781 45.238 84 39 2 1 83 657 734 1.65e-12 61.2
Msa1286140 MtrunA17_Chr8g0358161 53.846 52 24 0 20 71 213 264 1.81e-12 61.2
Msa1286140 MtrunA17_Chr8g0354421 56.604 53 22 1 17 68 526 578 1.91e-12 61.2
Msa1286140 MtrunA17_Chr8g0340081 54.000 50 23 0 20 69 674 723 2.10e-12 61.2
Msa1286140 MtrunA17_Chr2g0280761 59.184 49 20 0 23 71 1 49 2.26e-12 58.9
Msa1286140 MtrunA17_Chr8g0357961 54.902 51 23 0 20 70 651 701 2.29e-12 60.8
Msa1286140 MtrunA17_Chr8g0361161 59.615 52 20 1 20 71 211 261 2.35e-12 60.8
Msa1286140 MtrunA17_Chr4g0048731 55.769 52 23 0 20 71 213 264 2.42e-12 60.8
Msa1286140 MtrunA17_Chr4g0048711 55.769 52 23 0 20 71 96 147 2.49e-12 60.8
Msa1286140 MtrunA17_Chr4g0048711 56.863 51 22 0 21 71 254 304 3.44e-12 60.5
Msa1286140 MtrunA17_Chr8g0354431 56.604 53 22 1 17 68 505 557 2.50e-12 60.8
Msa1286140 MtrunA17_Chr8g0358061 51.923 52 25 0 20 71 223 274 2.98e-12 60.5
Msa1286140 MtrunA17_Chr4g0042351 45.000 60 33 0 12 71 612 671 3.41e-12 60.5
Msa1286140 MtrunA17_Chr8g0361441 51.923 52 25 0 20 71 673 724 3.59e-12 60.5
Msa1286140 MtrunA17_Chr1g0206331 56.863 51 22 0 20 70 502 552 3.87e-12 60.5
Msa1286140 MtrunA17_Chr8g0358031 52.941 51 24 0 20 70 646 696 4.00e-12 60.5
Msa1286140 MtrunA17_Chr2g0281241 55.556 54 24 0 17 70 218 271 4.01e-12 60.1
Msa1286140 MtrunA17_Chr8g0357991 52.941 51 24 0 20 70 652 702 4.04e-12 60.5
Msa1286140 MtrunA17_Chr3g0093161 54.902 51 23 0 20 70 664 714 4.54e-12 60.1
Msa1286140 MtrunA17_Chr4g0042441 48.980 49 25 0 20 68 667 715 4.86e-12 60.1
Msa1286140 MtrunA17_Chr8g0357901 49.020 51 26 0 22 72 17 67 5.02e-12 56.6
Msa1286140 MtrunA17_Chr8g0354441 57.143 49 21 0 20 68 517 565 5.56e-12 59.7
Msa1286140 MtrunA17_Chr2g0280791 56.863 51 21 1 20 70 688 737 5.69e-12 59.7
Msa1286140 MtrunA17_Chr3g0081071 57.143 49 21 0 20 68 96 144 5.70e-12 59.3
Msa1286140 MtrunA17_Chr2g0318811 56.863 51 21 1 20 70 667 716 6.02e-12 59.7
Msa1286140 MtrunA17_Chr7g0236341 53.846 52 23 1 20 71 685 735 6.03e-12 59.7
Msa1286140 MtrunA17_Chr1g0205771 50.980 51 25 0 20 70 510 560 6.14e-12 59.7
Msa1286140 MtrunA17_Chr5g0428101 52.941 51 24 0 20 70 668 718 6.14e-12 59.7
Msa1286140 MtrunA17_Chr2g0280801 56.863 51 21 1 20 70 694 743 6.72e-12 59.7
Msa1286140 MtrunA17_Chr2g0280701 58.491 53 22 0 21 73 668 720 6.84e-12 59.7
Msa1286140 MtrunA17_Chr1g0206351 58.537 41 17 0 20 60 520 560 9.98e-12 59.3
Msa1286140 MtrunA17_Chr6g0474831 49.180 61 30 1 12 71 215 275 1.05e-11 58.9
Msa1286140 MtrunA17_Chr3g0082941 57.143 49 21 0 20 68 340 388 1.10e-11 58.9
Msa1286140 MtrunA17_Chr8g0340091 54.000 50 23 0 20 69 699 748 1.10e-11 58.9
Msa1286140 MtrunA17_Chr8g0359581 48.333 60 25 1 1 54 55 114 1.27e-11 57.4
Msa1286140 MtrunA17_Chr1g0154311 53.333 45 21 0 20 64 518 562 1.39e-11 58.9
Msa1286140 MtrunA17_Chr5g0428571 49.231 65 32 1 20 83 465 529 1.60e-11 58.5
Msa1286140 MtrunA17_Chr4g0065541 46.875 64 32 1 5 68 592 653 2.09e-11 58.2
Msa1286140 MtrunA17_Chr7g0236631 53.333 45 21 0 20 64 21 65 2.34e-11 54.7
Msa1286140 MtrunA17_Chr8g0358141 50.000 52 26 0 20 71 245 296 2.36e-11 58.2
Msa1286140 MtrunA17_Chr5g0428111 50.980 51 25 0 20 70 670 720 2.48e-11 58.2
Msa1286140 MtrunA17_Chr8g0354351 52.941 51 24 0 20 70 500 550 2.59e-11 58.2
Msa1286140 MtrunA17_Chr7g0261141 47.059 51 27 0 18 68 9 59 2.66e-11 53.9
Msa1286140 MtrunA17_Chr1g0154371 65.789 38 13 0 20 57 523 560 2.78e-11 57.8
Msa1286140 MtrunA17_Chr7g0236121 57.407 54 22 1 20 73 671 723 2.84e-11 57.8
Msa1286140 MtrunA17_Chr5g0422921 50.980 51 25 0 20 70 665 715 3.16e-11 57.8
Msa1286140 MtrunA17_Chr8g0361431 50.943 53 22 2 20 70 651 701 3.25e-11 57.8
Msa1286140 MtrunA17_Chr7g0236201 57.407 54 22 1 20 73 637 689 3.31e-11 57.8
Msa1286140 MtrunA17_Chr5g0428521 39.241 79 44 2 1 77 150 226 3.57e-11 57.0
Msa1286140 MtrunA17_Chr6g0475201 47.368 57 30 0 12 68 733 789 3.64e-11 57.4
Msa1286140 MtrunA17_Chr1g0206461 55.102 49 22 0 20 68 500 548 3.76e-11 57.4
Msa1286140 MtrunA17_Chr5g0434141 52.000 50 24 0 20 69 761 810 4.48e-11 57.4
Msa1286140 MtrunA17_Chr6g0475131 47.368 57 30 0 12 68 168 224 4.75e-11 57.0
Msa1286140 MtrunA17_Chr4g0048571 45.588 68 36 1 2 69 86 152 4.87e-11 56.2
Msa1286140 MtrunA17_Chr4g0042391 48.077 52 27 0 20 71 620 671 4.89e-11 57.4
Msa1286140 MtrunA17_Chr4g0042381 48.077 52 27 0 20 71 620 671 5.23e-11 57.0
Msa1286140 MtrunA17_Chr1g0206431 49.123 57 29 0 12 68 44 100 6.09e-11 55.8
Msa1286140 MtrunA17_Chr8g0357781 51.020 49 24 0 23 71 1 49 6.69e-11 55.1
Msa1286140 MtrunA17_Chr8g0354391 47.619 63 32 1 6 68 88 149 7.43e-11 56.2
Msa1286140 MtrunA17_Chr8g0354281 53.061 49 23 0 20 68 502 550 7.81e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286140 AT4G27300.1 63.462 52 19 0 20 71 666 717 6.15e-18 77.4
Msa1286140 AT4G03230.3 62.745 51 19 0 20 70 691 741 1.09e-16 73.6
Msa1286140 AT4G03230.7 62.745 51 19 0 20 70 699 749 1.09e-16 73.6
Msa1286140 AT4G03230.2 62.745 51 19 0 20 70 756 806 1.09e-16 73.6
Msa1286140 AT4G03230.6 62.745 51 19 0 20 70 756 806 1.09e-16 73.6
Msa1286140 AT4G03230.5 62.745 51 19 0 20 70 784 834 1.09e-16 73.6
Msa1286140 AT4G03230.4 62.745 51 19 0 20 70 856 906 1.10e-16 73.6
Msa1286140 AT4G03230.1 62.745 51 19 0 20 70 856 906 1.10e-16 73.6
Msa1286140 AT4G03230.8 62.745 51 19 0 20 70 698 748 1.13e-16 73.6
Msa1286140 AT4G27290.1 60.784 51 20 0 20 70 631 681 1.66e-16 73.2
Msa1286140 AT4G27290.2 60.784 51 20 0 20 70 677 727 2.09e-16 72.8
Msa1286140 AT1G65790.1 63.462 52 19 0 20 71 685 736 4.17e-16 72.0
Msa1286140 AT1G65800.1 63.462 52 19 0 20 71 689 740 4.43e-16 72.0
Msa1286140 AT4G21380.1 61.538 52 20 0 20 71 692 743 3.02e-15 69.7
Msa1286140 AT4G23180.2 47.619 63 33 0 20 82 514 576 1.75e-14 67.4
Msa1286140 AT4G23150.1 56.863 51 22 0 20 70 502 552 2.30e-14 67.0
Msa1286140 AT1G11340.3 58.491 53 20 1 20 72 749 799 4.83e-14 66.2
Msa1286140 AT1G11340.1 57.692 52 20 1 20 71 681 730 7.73e-14 65.5
Msa1286140 AT4G23230.1 54.902 51 23 0 20 70 503 553 7.91e-14 65.5
Msa1286140 AT4G11530.1 59.615 52 20 1 20 70 518 569 1.62e-13 64.7
Msa1286140 AT1G11410.2 44.156 77 33 2 20 88 574 648 1.88e-13 64.3
Msa1286140 AT1G11410.3 44.156 77 33 2 20 88 665 739 1.89e-13 64.3
Msa1286140 AT1G11410.1 44.156 77 33 2 20 88 689 763 1.90e-13 64.3
Msa1286140 AT1G11410.4 44.156 77 33 2 20 88 696 770 1.90e-13 64.3
Msa1286140 AT4G23140.1 56.863 51 22 0 20 70 517 567 1.97e-13 64.3
Msa1286140 AT4G23160.1 56.863 51 22 0 20 70 525 575 2.23e-13 64.3
Msa1286140 AT1G11330.3 54.545 55 25 0 20 74 690 744 2.98e-13 63.9
Msa1286140 AT4G23180.1 52.941 51 24 0 20 70 514 564 3.33e-13 63.9
Msa1286140 AT4G23200.1 52.830 53 24 1 20 71 502 554 3.42e-13 63.5
Msa1286140 AT4G23140.2 54.386 57 25 1 15 70 517 573 4.29e-13 63.5
Msa1286140 AT4G11470.2 54.717 53 23 1 20 71 510 562 1.32e-12 62.0
Msa1286140 AT4G11470.1 54.717 53 23 1 20 71 505 557 1.41e-12 62.0
Msa1286140 AT4G11460.2 52.830 53 24 1 20 71 437 489 1.64e-12 61.6
Msa1286140 AT4G11460.3 52.830 53 24 1 20 71 512 564 1.65e-12 61.6
Msa1286140 AT4G11460.1 52.830 53 24 1 20 71 515 567 1.65e-12 61.6
Msa1286140 AT4G05200.1 43.077 65 31 1 20 84 513 571 1.94e-12 61.6
Msa1286140 AT4G05200.2 43.077 65 31 1 20 84 531 589 1.95e-12 61.6
Msa1286140 AT4G23240.2 56.604 53 22 1 20 71 194 246 2.54e-12 61.2
Msa1286140 AT4G23290.1 54.717 53 23 1 20 71 486 538 2.55e-12 61.2
Msa1286140 AT4G23290.2 54.717 53 23 1 20 71 529 581 2.56e-12 61.2
Msa1286140 AT4G23130.3 52.727 55 25 1 20 73 510 564 3.19e-12 60.8
Msa1286140 AT4G23190.2 49.123 57 26 1 20 73 516 572 3.70e-12 60.8
Msa1286140 AT4G11480.4 52.830 53 24 1 20 71 487 539 5.34e-12 60.5
Msa1286140 AT4G11480.3 52.830 53 24 1 20 71 490 542 5.34e-12 60.5
Msa1286140 AT4G11480.1 52.830 53 24 1 20 71 495 547 5.35e-12 60.5
Msa1286140 AT4G23260.1 55.102 49 22 0 20 68 505 553 5.73e-12 60.1
Msa1286140 AT4G23260.2 55.102 49 22 0 20 68 494 542 6.01e-12 60.1
Msa1286140 AT4G23250.3 55.102 49 22 0 20 68 520 568 8.18e-12 59.7
Msa1286140 AT4G23250.1 55.102 49 22 0 20 68 525 573 9.17e-12 59.7
Msa1286140 AT4G23250.2 55.102 49 22 0 20 68 520 568 9.34e-12 59.7
Msa1286140 AT4G23190.3 53.333 45 21 0 20 64 438 482 1.60e-11 58.9
Msa1286140 AT4G23190.4 53.333 45 21 0 20 64 438 482 1.60e-11 58.9
Msa1286140 AT4G23190.1 53.333 45 21 0 20 64 516 560 1.61e-11 58.9
Msa1286140 AT1G11330.1 54.902 51 23 0 20 70 688 738 1.94e-11 58.9
Msa1286140 AT1G11330.2 54.902 51 23 0 20 70 690 740 1.94e-11 58.9
Msa1286140 AT1G11280.3 54.902 51 23 0 20 70 659 709 2.08e-11 58.5
Msa1286140 AT1G11280.1 54.902 51 23 0 20 70 681 731 2.08e-11 58.5
Msa1286140 AT1G11280.4 54.902 51 23 0 20 70 669 719 2.10e-11 58.5
Msa1286140 AT1G11280.2 54.902 51 23 0 20 70 671 721 2.10e-11 58.5
Msa1286140 AT4G23270.2 53.061 49 23 0 20 68 492 540 2.83e-11 58.2
Msa1286140 AT4G23270.1 53.061 49 23 0 20 68 492 540 2.83e-11 58.2
Msa1286140 AT4G23270.3 53.061 49 23 0 20 68 492 540 2.83e-11 58.2
Msa1286140 AT4G00970.2 52.830 53 25 0 20 72 512 564 2.89e-11 58.2
Msa1286140 AT4G23130.1 53.061 49 23 0 20 68 506 554 3.03e-11 58.2
Msa1286140 AT4G23130.2 53.061 49 23 0 20 68 510 558 3.04e-11 58.2
Msa1286140 AT4G23240.1 48.529 68 31 1 20 87 501 564 3.28e-11 58.2
Msa1286140 AT4G00970.1 53.846 52 24 0 20 71 512 563 4.66e-11 57.8
Msa1286140 AT1G61430.10 51.786 56 27 0 20 75 632 687 5.80e-11 57.4
Msa1286140 AT4G38830.1 61.538 39 15 0 20 58 511 549 6.69e-11 57.4
Msa1286140 AT4G21366.1 53.061 49 23 0 23 71 1 49 7.63e-11 54.3
Msa1286140 AT4G23310.2 51.020 49 24 0 20 68 634 682 9.14e-11 57.0
Msa1286140 AT4G23310.1 51.020 49 24 0 20 68 674 722 9.16e-11 57.0

Find 16 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAGTGGAAATAAGATTA+AGG 0.250594 8_3:+33104741 Msa1286140:CDS
GAACATTCTCTCAATCTTCT+TGG 0.250889 8_3:+33104778 Msa1286140:CDS
AGCTTGGACTACTTCATCTT+TGG 0.307095 8_3:+33104222 Msa1286140:CDS
AAATCCGATGTGTTCAGTTT+TGG 0.330127 8_3:+33104700 Msa1286140:CDS
ACTGAACACATCGGATTTCA+TGG 0.341173 8_3:-33104695 None:intergenic
ATTAGTGGAAATAAGATTAA+GGG 0.369260 8_3:+33104742 Msa1286140:CDS
GAAGTAGTCCAAGCTCTTGT+TGG 0.436675 8_3:-33104214 None:intergenic
ATAAAATTGGAGATAATTAG+TGG 0.452288 8_3:+33104727 Msa1286140:CDS
TGGATTGAGTCATATCTTGA+TGG 0.455481 8_3:+33104835 Msa1286140:CDS
AGAAGATTGAGAGAATGTTC+TGG 0.469678 8_3:-33104775 None:intergenic
AAGTAGTCCAAGCTCTTGTT+GGG 0.488770 8_3:-33104213 None:intergenic
ATACATGCCCAACAAGAGCT+TGG 0.545459 8_3:+33104206 Msa1286140:CDS
CGACCACGCACTGCATACGC+TGG 0.591171 8_3:-33104667 None:intergenic
CCTCCAGCGTATGCAGTGCG+TGG 0.655306 8_3:+33104664 Msa1286140:CDS
TACGCCAAAACTGAACACAT+CGG 0.656116 8_3:-33104704 None:intergenic
CCACGCACTGCATACGCTGG+AGG 0.665671 8_3:-33104664 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTTAGTAATTATAAAA+AGG - chr8_3:33104254-33104273 None:intergenic 10.0%
!!! AATTTGCTTTATTAATCTAT+AGG - chr8_3:33104277-33104296 None:intergenic 15.0%
!!! CTGTTATAATTTTACTTATT+TGG + chr8_3:33104815-33104834 Msa1286140:CDS 15.0%
!! ATAAAATTGGAGATAATTAG+TGG + chr8_3:33104727-33104746 Msa1286140:CDS 20.0%
!! AATTAGTGGAAATAAGATTA+AGG + chr8_3:33104741-33104760 Msa1286140:CDS 20.0%
!! ATTAGTGGAAATAAGATTAA+GGG + chr8_3:33104742-33104761 Msa1286140:CDS 20.0%
! AAAGCAAATTGTATGCTAAA+CGG + chr8_3:33104287-33104306 Msa1286140:intron 25.0%
!! ATTCCAAAAATCTCAGATTT+TGG + chr8_3:33104480-33104499 Msa1286140:intron 25.0%
! AGTTACTAAGTACAAAAAGA+AGG - chr8_3:33104578-33104597 None:intergenic 25.0%
!!! TGAGATTTTTGGAATCATAC+TGG - chr8_3:33104475-33104494 None:intergenic 30.0%
!! AAGCCAAAATCTGAGATTTT+TGG - chr8_3:33104486-33104505 None:intergenic 30.0%
TGAAGCCAAAACCAAAAAAA+TGG + chr8_3:33104533-33104552 Msa1286140:intron 30.0%
TCTTCAAACAATACTTTCAG+TGG + chr8_3:33104634-33104653 Msa1286140:intron 30.0%
CTTCAAACAATACTTTCAGT+GGG + chr8_3:33104635-33104654 Msa1286140:intron 30.0%
!! CAGTTTTGGCGTAATAAAAT+TGG + chr8_3:33104714-33104733 Msa1286140:CDS 30.0%
! TAATTCTGAGTCTAGAGTCT+TGG - chr8_3:33104413-33104432 None:intergenic 35.0%
GGCATCTAATAGGATATTGT+TGG - chr8_3:33104454-33104473 None:intergenic 35.0%
GAATCATACTGGCATCTAAT+AGG - chr8_3:33104464-33104483 None:intergenic 35.0%
GCCAAAACCAAAAAAATGGT+CGG + chr8_3:33104537-33104556 Msa1286140:intron 35.0%
CCAAAACCAAAAAAATGGTC+GGG + chr8_3:33104538-33104557 Msa1286140:intron 35.0%
! AAATCCGATGTGTTCAGTTT+TGG + chr8_3:33104700-33104719 Msa1286140:CDS 35.0%
AGAAGATTGAGAGAATGTTC+TGG - chr8_3:33104778-33104797 None:intergenic 35.0%
GAACATTCTCTCAATCTTCT+TGG + chr8_3:33104778-33104797 Msa1286140:CDS 35.0%
TGGATTGAGTCATATCTTGA+TGG + chr8_3:33104835-33104854 Msa1286140:CDS 35.0%
AAGTAGTCCAAGCTCTTGTT+GGG - chr8_3:33104216-33104235 None:intergenic 40.0%
AGCTTGGACTACTTCATCTT+TGG + chr8_3:33104222-33104241 Msa1286140:CDS 40.0%
TGCCAGCAATAATGTTGGAA+CGG - chr8_3:33104365-33104384 None:intergenic 40.0%
AGCAATGCCAGCAATAATGT+TGG - chr8_3:33104370-33104389 None:intergenic 40.0%
ATTATTGCTGGCATTGCTAG+AGG + chr8_3:33104372-33104391 Msa1286140:intron 40.0%
!!! TCTCAGATTTTGGCTTAGCT+CGG + chr8_3:33104490-33104509 Msa1286140:intron 40.0%
!!! CCCGACCATTTTTTTGGTTT+TGG - chr8_3:33104541-33104560 None:intergenic 40.0%
!!! CTATGTCCCGACCATTTTTT+TGG - chr8_3:33104547-33104566 None:intergenic 40.0%
ACTGAACACATCGGATTTCA+TGG - chr8_3:33104698-33104717 None:intergenic 40.0%
TACGCCAAAACTGAACACAT+CGG - chr8_3:33104707-33104726 None:intergenic 40.0%
ATACATGCCCAACAAGAGCT+TGG + chr8_3:33104206-33104225 Msa1286140:CDS 45.0%
GAAGTAGTCCAAGCTCTTGT+TGG - chr8_3:33104217-33104236 None:intergenic 45.0%
TGAAACCAGAAGCAAACTCC+TGG + chr8_3:33104329-33104348 Msa1286140:intron 45.0%
CACCGTTCCAACATTATTGC+TGG + chr8_3:33104360-33104379 Msa1286140:intron 45.0%
TTGGCTTAGCTCGGACATTT+AGG + chr8_3:33104499-33104518 Msa1286140:intron 45.0%
TGGCTTAGCTCGGACATTTA+GGG + chr8_3:33104500-33104519 Msa1286140:intron 45.0%
AATGTTGGAACGGTGAGTCC+AGG - chr8_3:33104355-33104374 None:intergenic 50.0%
GGCTTAGCTCGGACATTTAG+GGG + chr8_3:33104501-33104520 Msa1286140:intron 50.0%
! CCAGGCCAGGAGTTTGCTTC+TGG - chr8_3:33104337-33104356 None:intergenic 60.0%
CCAGAAGCAAACTCCTGGCC+TGG + chr8_3:33104334-33104353 Msa1286140:intron 60.0%
TGGAACGGTGAGTCCAGGCC+AGG - chr8_3:33104350-33104369 None:intergenic 65.0%
CCACGCACTGCATACGCTGG+AGG - chr8_3:33104667-33104686 None:intergenic 65.0%
CCTCCAGCGTATGCAGTGCG+TGG + chr8_3:33104664-33104683 Msa1286140:CDS 65.0%
CGACCACGCACTGCATACGC+TGG - chr8_3:33104670-33104689 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 33104189 33104867 33104189 ID=Msa1286140;Name=Msa1286140
chr8_3 mRNA 33104189 33104867 33104189 ID=Msa1286140-mRNA-1;Parent=Msa1286140;Name=Msa1286140-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0|0|1|0|0.5|2|0|91
chr8_3 exon 33104189 33104247 33104189 ID=Msa1286140-mRNA-1:exon:10138;Parent=Msa1286140-mRNA-1
chr8_3 exon 33104654 33104867 33104654 ID=Msa1286140-mRNA-1:exon:10139;Parent=Msa1286140-mRNA-1
chr8_3 CDS 33104189 33104247 33104189 ID=Msa1286140-mRNA-1:cds;Parent=Msa1286140-mRNA-1
chr8_3 CDS 33104654 33104867 33104654 ID=Msa1286140-mRNA-1:cds;Parent=Msa1286140-mRNA-1
Gene Sequence

>Msa1286140

ATGTTATTGATATATGAATACATGCCCAACAAGAGCTTGGACTACTTCATCTTTGGTTTTGGGTACATGCCTCCAGCGTATGCAGTGCGTGGTCGTTACTCCATGAAATCCGATGTGTTCAGTTTTGGCGTAATAAAATTGGAGATAATTAGTGGAAATAAGATTAAGGGTTTTTTTGAACCAGAACATTCTCTCAATCTTCTTGGACATGTAACTATAACTGTTATAATTTTACTTATTTGGATTGAGTCATATCTTGATGGATTCAAAGAT
Protein sequence

>Msa1286140

MLLIYEYMPNKSLDYFIFGFGYMPPAYAVRGRYSMKSDVFSFGVIKLEIISGNKIKGFFEPEHSLNLLGHVTITVIILLIWIESYLDGFKD