AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1286840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286840 MtrunA17_Chr8g0360761 68.148 135 16 2 1 135 1 108 4.84e-56 173
Msa1286840 MtrunA17_Chr2g0293491 33.939 165 77 5 9 141 39 203 5.25e-24 94.0
Msa1286840 MtrunA17_Chr4g0051261 33.735 166 78 5 8 141 38 203 1.77e-23 92.8
Msa1286840 MtrunA17_Chr6g0453711 32.680 153 70 5 22 141 158 310 4.88e-20 85.1
Msa1286840 MtrunA17_Chr8g0358921 34.132 167 73 4 12 141 85 251 1.54e-19 82.8
Msa1286840 MtrunA17_Chr7g0218071 33.113 151 68 5 24 141 170 320 1.55e-19 83.6
Msa1286840 MtrunA17_Chr1g0201571 32.544 169 74 8 11 141 160 326 3.60e-18 79.7
Msa1286840 MtrunA17_Chr4g0061141 31.373 153 72 5 22 141 153 305 3.61e-18 79.7
Msa1286840 MtrunA17_Chr5g0396361 32.026 153 71 4 22 141 188 340 8.78e-18 78.6
Msa1286840 MtrunA17_Chr4g0057791 32.099 162 76 5 14 141 258 419 2.30e-17 77.4
Msa1286840 MtrunA17_Chr8g0387401 30.128 156 76 5 19 141 97 252 7.53e-17 75.9
Msa1286840 MtrunA17_Chr8g0383451 30.719 153 73 5 22 141 46 198 9.60e-17 75.5
Msa1286840 MtrunA17_Chr3g0093571 28.000 175 79 5 14 141 65 239 9.62e-17 75.5
Msa1286840 MtrunA17_Chr5g0409421 29.139 151 79 2 19 141 48 198 1.63e-16 75.1
Msa1286840 MtrunA17_Chr3g0093391 27.429 175 80 5 14 141 63 237 2.62e-16 74.3
Msa1286840 MtrunA17_Chr8g0377071 30.000 160 80 6 14 141 425 584 2.80e-16 74.3
Msa1286840 MtrunA17_Chr1g0187371 32.544 169 73 7 9 136 52 220 3.24e-16 73.6
Msa1286840 MtrunA17_Chr3g0099951 31.818 154 75 5 17 140 59 212 4.61e-16 73.6
Msa1286840 MtrunA17_Chr8g0385971 31.788 151 70 6 24 141 140 290 5.08e-16 73.6
Msa1286840 MtrunA17_Chr7g0273601 29.940 167 81 6 11 141 141 307 5.52e-16 73.6
Msa1286840 MtrunA17_Chr2g0324461 31.126 151 71 5 24 141 191 341 6.08e-16 73.6
Msa1286840 MtrunA17_Chr4g0002001 35.156 128 47 4 46 137 89 216 6.19e-16 72.8
Msa1286840 MtrunA17_Chr8g0369451 29.139 151 79 2 19 141 48 198 7.96e-16 73.2
Msa1286840 MtrunA17_Chr1g0191221 30.464 151 72 5 24 141 172 322 2.76e-15 71.6
Msa1286840 MtrunA17_Chr4g0040141 29.677 155 72 5 22 141 177 329 4.59e-15 70.9
Msa1286840 MtrunA17_Chr3g0131861 31.788 151 70 6 24 141 147 297 4.80e-15 70.9
Msa1286840 MtrunA17_Chr3g0122781 29.375 160 81 6 14 141 456 615 6.00e-15 70.5
Msa1286840 MtrunA17_Chr4g0066691 29.139 151 74 5 24 141 154 304 6.65e-15 70.5
Msa1286840 MtrunA17_Chr5g0397261 28.205 156 79 5 19 141 86 241 1.10e-14 69.7
Msa1286840 MtrunA17_Chr2g0286421 30.464 151 72 5 24 141 152 302 1.23e-14 69.7
Msa1286840 MtrunA17_Chr2g0317501 28.105 153 77 4 22 141 182 334 1.31e-14 69.7
Msa1286840 MtrunA17_Chr6g0483151 30.519 154 77 4 17 140 57 210 2.90e-14 68.6
Msa1286840 MtrunA17_Chr8g0378831 29.299 157 77 4 19 141 77 233 5.14e-14 67.8
Msa1286840 MtrunA17_Chr3g0141871 29.299 157 77 4 19 141 77 233 5.25e-14 67.8
Msa1286840 MtrunA17_Chr8g0339221 25.325 154 87 2 16 141 45 198 1.86e-13 66.2
Msa1286840 MtrunA17_Chr4g0030151 27.389 157 79 5 19 141 84 239 2.02e-13 65.9
Msa1286840 MtrunA17_Chr4g0045841 26.282 156 82 4 19 141 100 255 2.17e-13 65.9
Msa1286840 MtrunA17_Chr6g0482681 32.317 164 69 8 18 141 133 294 2.46e-13 65.9
Msa1286840 MtrunA17_Chr8g0392811 31.707 164 70 8 18 141 161 322 3.90e-13 65.5
Msa1286840 MtrunA17_Chr4g0030231 26.282 156 82 4 19 141 85 240 5.24e-13 64.7
Msa1286840 MtrunA17_Chr8g0386981 26.190 168 95 3 2 140 439 606 1.90e-12 63.5
Msa1286840 MtrunA17_Chr3g0102571 36.559 93 41 2 67 141 189 281 2.14e-12 63.2
Msa1286840 MtrunA17_Chr4g0065851 31.098 164 71 8 18 141 69 230 2.30e-12 63.2
Msa1286840 MtrunA17_Chr2g0287271 31.098 164 71 9 18 141 69 230 2.68e-12 62.8
Msa1286840 MtrunA17_Chr7g0248461 25.641 156 83 4 19 141 83 238 4.44e-12 62.0
Msa1286840 MtrunA17_Chr3g0130781 36.364 99 39 3 67 141 132 230 3.59e-11 59.7
Msa1286840 MtrunA17_Chr3g0096631 30.061 163 74 8 18 141 69 230 4.77e-11 59.3
Msa1286840 MtrunA17_Chr8g0348481 25.000 156 84 4 19 141 82 237 5.26e-11 58.9
Msa1286840 MtrunA17_Chr8g0359091 31.098 164 71 7 18 141 70 231 8.85e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1286840 AT3G48750.1 34.337 166 77 5 8 141 38 203 8.37e-25 96.7
Msa1286840 AT5G39420.1 31.953 169 75 6 11 141 139 305 3.23e-19 83.2
Msa1286840 AT2G38620.3 30.682 176 73 6 15 141 45 220 7.75e-19 80.1
Msa1286840 AT2G38620.1 30.682 176 73 6 15 141 45 220 7.75e-19 80.1
Msa1286840 AT2G38620.2 31.395 172 69 6 15 137 45 216 2.55e-18 79.7
Msa1286840 AT3G01085.3 31.210 157 75 4 18 141 159 315 3.58e-18 80.1
Msa1286840 AT3G01085.1 31.210 157 75 4 18 141 159 315 3.69e-18 80.1
Msa1286840 AT3G01085.2 31.210 157 75 4 18 141 159 315 3.69e-18 80.1
Msa1286840 AT1G76540.1 33.728 169 71 8 9 136 48 216 2.09e-17 77.0
Msa1286840 AT1G18670.1 31.579 152 70 5 24 141 181 332 3.21e-17 77.4
Msa1286840 AT1G18670.2 31.579 152 70 5 24 141 181 332 3.28e-17 77.4
Msa1286840 AT1G18670.3 31.579 152 70 5 24 141 181 332 3.51e-17 77.4
Msa1286840 AT1G66750.1 33.117 154 73 4 17 140 56 209 7.52e-17 75.9
Msa1286840 AT1G20930.1 31.609 174 78 6 9 141 50 223 9.27e-17 75.5
Msa1286840 AT3G54180.1 34.848 132 50 4 42 137 83 214 1.98e-16 74.3
Msa1286840 AT1G74330.1 30.719 153 71 5 24 141 171 323 2.77e-16 74.7
Msa1286840 AT1G74330.3 30.719 153 71 5 24 141 171 323 2.93e-16 74.7
Msa1286840 AT2G38620.4 43.333 90 32 2 67 137 65 154 3.11e-16 73.2
Msa1286840 AT1G74330.2 30.719 153 71 5 24 141 171 323 3.29e-16 74.7
Msa1286840 AT4G19110.2 28.477 151 80 2 19 141 48 198 3.41e-16 74.3
Msa1286840 AT4G19110.1 28.477 151 80 2 19 141 48 198 3.50e-16 74.3
Msa1286840 AT5G64960.2 26.136 176 83 4 13 141 12 187 3.67e-16 74.3
Msa1286840 AT1G33770.1 31.373 153 72 4 22 141 189 341 3.92e-16 74.3
Msa1286840 AT5G50860.1 31.373 153 72 6 22 141 162 314 3.96e-16 74.3
Msa1286840 AT1G54610.1 28.105 153 77 4 22 141 166 318 6.23e-16 73.9
Msa1286840 AT1G54610.3 28.105 153 77 4 22 141 166 318 6.23e-16 73.9
Msa1286840 AT1G54610.2 28.105 153 77 4 22 141 166 318 6.23e-16 73.9
Msa1286840 AT5G10270.1 25.714 175 83 4 14 141 66 240 6.24e-16 73.6
Msa1286840 AT1G18040.1 31.169 154 76 4 17 140 55 208 7.37e-16 73.2
Msa1286840 AT5G45430.1 30.464 151 77 3 19 141 48 198 7.40e-16 73.6
Msa1286840 AT5G45430.2 30.464 151 77 3 19 141 48 198 8.06e-16 73.6
Msa1286840 AT5G64960.1 25.714 175 83 4 14 141 66 240 1.21e-15 72.8
Msa1286840 AT5G50860.2 31.788 151 70 6 24 141 164 314 1.98e-15 72.4
Msa1286840 AT5G63370.9 30.864 162 77 6 14 141 207 367 3.16e-15 71.6
Msa1286840 AT1G71530.1 29.032 155 73 4 22 141 195 347 3.17e-15 71.6
Msa1286840 AT5G63370.2 30.864 162 77 6 14 141 207 367 3.64e-15 71.6
Msa1286840 AT5G63370.3 30.864 162 77 6 14 141 207 367 3.64e-15 71.6
Msa1286840 AT1G53050.2 28.105 153 77 4 22 141 182 334 3.76e-15 71.6
Msa1286840 AT1G53050.1 28.105 153 77 4 22 141 182 334 3.76e-15 71.6
Msa1286840 AT5G63370.5 30.864 162 77 6 14 141 341 501 4.15e-15 71.2
Msa1286840 AT5G63370.10 30.864 162 77 6 14 141 341 501 4.15e-15 71.2
Msa1286840 AT5G63370.7 30.864 162 77 6 14 141 341 501 4.57e-15 71.2
Msa1286840 AT5G63370.8 30.864 162 77 6 14 141 341 501 4.57e-15 71.2
Msa1286840 AT5G63370.4 30.864 162 77 6 14 141 341 501 4.57e-15 71.2
Msa1286840 AT5G63370.6 30.864 162 77 6 14 141 341 501 4.57e-15 71.2
Msa1286840 AT5G63370.1 30.864 162 77 6 14 141 341 501 4.57e-15 71.2
Msa1286840 AT1G71530.4 29.412 153 71 4 24 141 197 347 5.37e-15 70.9
Msa1286840 AT3G59790.1 28.205 156 79 4 19 141 105 260 7.42e-15 70.5
Msa1286840 AT1G71530.3 29.412 153 71 4 24 141 197 347 7.45e-15 70.5
Msa1286840 AT1G71530.2 29.412 153 71 4 24 141 197 347 9.31e-15 70.5
Msa1286840 AT1G59580.2 31.210 157 74 4 19 141 77 233 1.19e-14 69.7
Msa1286840 AT1G59580.1 31.210 157 74 4 19 141 77 233 1.19e-14 69.7
Msa1286840 AT4G10010.1 30.968 155 70 6 22 141 24 176 1.25e-14 70.1
Msa1286840 AT3G05050.3 30.137 146 69 3 24 136 188 333 1.29e-14 70.1
Msa1286840 AT3G05050.2 30.137 146 69 3 24 136 188 333 1.29e-14 70.1
Msa1286840 AT3G05050.1 30.137 146 69 3 24 136 188 333 1.29e-14 70.1
Msa1286840 AT4G10010.2 29.677 155 72 5 22 141 204 356 1.56e-14 69.7
Msa1286840 AT1G73690.1 29.221 154 79 4 17 140 54 207 1.62e-14 69.7
Msa1286840 AT2G18170.1 28.662 157 78 3 19 141 77 233 1.78e-14 69.3
Msa1286840 AT4G19110.3 28.777 139 71 2 31 141 4 142 1.90e-14 69.3
Msa1286840 AT4G36450.1 28.571 154 79 3 19 141 77 230 3.10e-14 68.6
Msa1286840 AT1G07880.1 28.846 156 78 4 19 141 78 233 3.72e-14 67.4
Msa1286840 AT4G22940.1 27.273 165 86 6 11 141 140 304 4.04e-14 68.6
Msa1286840 AT1G10210.2 29.936 157 76 4 19 141 77 233 4.46e-14 68.2
Msa1286840 AT1G10210.3 29.936 157 76 4 19 141 77 233 4.46e-14 68.2
Msa1286840 AT1G10210.1 29.936 157 76 4 19 141 77 233 4.46e-14 68.2
Msa1286840 AT4G22940.2 27.273 165 86 6 11 141 140 304 4.66e-14 68.6
Msa1286840 AT1G67580.1 28.750 160 82 6 14 141 446 605 5.21e-14 68.2
Msa1286840 AT1G67580.2 28.750 160 82 6 14 141 446 605 5.21e-14 68.2
Msa1286840 AT1G07880.2 27.273 154 79 4 19 139 78 231 7.71e-14 67.4
Msa1286840 AT4G11330.1 28.205 156 79 5 19 141 88 243 7.83e-14 67.4
Msa1286840 AT3G18040.3 32.927 164 68 8 18 141 176 337 2.07e-13 66.6
Msa1286840 AT3G18040.5 32.927 164 68 8 18 141 62 223 2.41e-13 66.2
Msa1286840 AT3G18040.1 32.927 164 68 8 18 141 67 228 2.56e-13 66.2
Msa1286840 AT3G18040.4 32.927 164 68 8 18 141 176 337 2.78e-13 66.2
Msa1286840 AT1G53570.5 29.801 151 85 3 2 140 245 386 3.21e-13 65.9
Msa1286840 AT1G03740.1 27.815 151 76 5 24 141 263 413 5.08e-13 65.5
Msa1286840 AT1G03740.2 27.815 151 76 5 24 141 263 413 5.19e-13 65.5
Msa1286840 AT5G66850.2 28.070 171 87 4 6 140 381 551 7.47e-13 65.1
Msa1286840 AT5G66850.1 28.070 171 87 4 6 140 381 551 7.52e-13 65.1
Msa1286840 AT2G43790.1 26.282 156 82 4 19 141 108 263 8.50e-13 64.7
Msa1286840 AT4G01370.1 26.923 156 81 4 19 141 88 243 1.09e-12 64.3
Msa1286840 AT2G01450.3 32.727 165 67 10 18 141 60 221 1.22e-12 64.3
Msa1286840 AT2G01450.1 32.727 165 67 10 18 141 60 221 1.22e-12 64.3
Msa1286840 AT2G01450.2 32.727 165 67 10 18 141 60 221 1.22e-12 64.3
Msa1286840 AT2G01450.4 32.727 165 67 10 18 141 60 221 1.22e-12 64.3
Msa1286840 AT2G46070.2 25.641 156 83 4 19 141 86 241 1.73e-12 63.5
Msa1286840 AT2G46070.3 25.641 156 83 4 19 141 120 275 2.32e-12 63.5
Msa1286840 AT2G46070.1 25.641 156 83 4 19 141 86 241 2.46e-12 63.2
Msa1286840 AT1G18150.3 31.098 164 71 8 18 141 148 309 2.88e-12 63.2
Msa1286840 AT1G18150.2 31.098 164 71 8 18 141 148 309 2.88e-12 63.2
Msa1286840 AT1G18150.1 31.098 164 71 8 18 141 148 309 2.88e-12 63.2
Msa1286840 AT1G09600.1 26.490 151 78 4 24 141 213 363 3.02e-12 63.2
Msa1286840 AT1G73670.1 31.707 164 70 8 18 141 134 295 4.09e-12 62.8
Msa1286840 AT5G19010.1 31.098 164 71 9 18 141 69 230 4.37e-12 62.8
Msa1286840 AT4G13020.4 27.815 151 81 2 19 141 48 198 4.74e-12 62.4
Msa1286840 AT4G13020.1 27.815 151 81 2 19 141 48 198 4.96e-12 62.4
Msa1286840 AT4G13020.6 27.815 151 81 2 19 141 48 198 5.05e-12 62.4
Msa1286840 AT4G13020.2 27.815 151 81 2 19 141 56 206 5.23e-12 62.4
Msa1286840 AT4G13020.3 27.815 151 81 2 19 141 56 206 5.23e-12 62.4
Msa1286840 AT5G63610.1 35.354 99 40 2 67 141 137 235 5.26e-12 62.4
Msa1286840 AT4G13020.7 27.815 151 81 2 19 141 48 198 5.96e-12 62.4
Msa1286840 AT1G53510.1 32.317 164 69 8 18 141 69 230 6.14e-12 62.4
Msa1286840 AT4G13020.5 27.517 149 80 2 21 141 59 207 6.16e-12 62.4
Msa1286840 AT4G13020.8 27.517 149 80 2 21 141 59 207 6.86e-12 62.0
Msa1286840 AT3G45640.1 26.752 157 80 5 19 141 83 238 7.64e-12 62.0
Msa1286840 AT3G14720.2 31.818 176 73 9 11 141 57 230 8.05e-12 62.0
Msa1286840 AT3G14720.3 31.818 176 73 9 11 141 57 230 8.05e-12 62.0
Msa1286840 AT5G44290.6 26.490 151 78 5 24 141 187 337 8.47e-12 62.0
Msa1286840 AT1G01560.4 26.282 156 82 4 19 141 85 240 9.04e-12 61.2
Msa1286840 AT1G01560.1 26.282 156 82 4 19 141 85 240 9.04e-12 61.2
Msa1286840 AT5G44290.7 26.490 151 78 5 24 141 187 337 1.04e-11 61.6
Msa1286840 AT3G14720.1 32.317 164 69 8 18 141 69 230 1.24e-11 61.6
Msa1286840 AT5G44290.2 27.815 151 76 6 24 141 187 337 1.36e-11 61.2
Msa1286840 AT5G44290.5 27.815 151 76 6 24 141 187 337 1.36e-11 61.2
Msa1286840 AT5G44290.4 27.815 151 76 6 24 141 187 337 1.36e-11 61.2
Msa1286840 AT5G44290.1 27.815 151 76 6 24 141 187 337 1.36e-11 61.2
Msa1286840 AT5G44290.3 27.815 151 76 6 24 141 187 337 1.36e-11 61.2
Msa1286840 AT1G01560.3 26.282 156 82 4 19 141 85 240 1.97e-11 60.8
Msa1286840 AT1G53570.7 27.381 168 93 4 2 140 245 412 2.31e-11 60.5
Msa1286840 AT1G53570.6 27.381 168 93 4 2 140 245 412 2.33e-11 60.5
Msa1286840 AT1G01560.2 26.282 156 82 4 19 141 85 240 2.42e-11 60.5
Msa1286840 AT2G42880.2 31.098 164 71 8 18 141 69 230 3.01e-11 60.5
Msa1286840 AT1G53570.4 27.381 168 93 4 2 140 245 412 3.41e-11 60.1
Msa1286840 AT1G53570.1 27.381 168 93 4 2 140 245 412 3.41e-11 60.1
Msa1286840 AT2G42880.1 31.098 164 71 8 18 141 69 230 3.51e-11 60.1
Msa1286840 AT1G53570.2 27.381 168 93 4 2 140 245 412 3.51e-11 60.1
Msa1286840 AT1G53570.3 27.381 168 93 4 2 140 245 412 3.51e-11 60.1

Find 28 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGTTGATATCTGGTCTA+TGG 0.259046 8_3:+35634884 Msa1286840:CDS
AAGATTGACCCGACAATATT+GGG 0.286282 8_3:-35633985 None:intergenic
ATCAACTGCAGTTGAGTAAT+TGG 0.289792 8_3:-35634871 None:intergenic
AAAGATTGACCCGACAATAT+TGG 0.297856 8_3:-35633986 None:intergenic
TCTGAAATCTAATAATATTC+TGG 0.322462 8_3:+35634639 Msa1286840:CDS
CTGCTATCTATTCAGATTGC+AGG 0.356258 8_3:+35634162 Msa1286840:intron
TAAAGCACCTGAACCTTTGA+TGG 0.361768 8_3:+35634842 Msa1286840:CDS
ACTCAACTGCAGTTGATATC+TGG 0.396574 8_3:+35634877 Msa1286840:CDS
GCATATTTGCTGAGATGCTT+AGG 0.419785 8_3:+35634910 Msa1286840:CDS
GTAGAACCCATCAAAGGTTC+AGG 0.434339 8_3:-35634849 None:intergenic
GTTCATCATTACCAATGAAC+AGG 0.435723 8_3:-35634939 None:intergenic
TTAGGCTCGAACCTGTTCAT+TGG 0.452136 8_3:+35634928 Msa1286840:CDS
TGAAGACGTGAAGCTCTCTC+TGG 0.455679 8_3:+35634577 Msa1286840:intron
TCGAAAACACGTCGCCAATC+CGG 0.480236 8_3:+35634243 Msa1286840:CDS
TTGGACCTCCCCAATATTGT+CGG 0.500470 8_3:+35633976 Msa1286840:CDS
ATTCAGATTGCAGGCAGTGA+TGG 0.510492 8_3:+35634171 Msa1286840:intron
GCAGTTGATATCTGGTCTAT+GGG 0.534989 8_3:+35634885 Msa1286840:CDS
GGTTCAGGTGCTTTATAGCA+CGG 0.550189 8_3:-35634834 None:intergenic
ACGTGAAGCTCTCTCTGGAT+TGG 0.552645 8_3:+35634582 Msa1286840:CDS
TGGACCTCCCCAATATTGTC+GGG 0.558796 8_3:+35633977 Msa1286840:CDS
AAAGCACCTGAACCTTTGAT+GGG 0.566858 8_3:+35634843 Msa1286840:CDS
GCAGTGATGGAAACTGAGGA+TGG 0.629963 8_3:+35634184 Msa1286840:CDS
AGATTGACCCGACAATATTG+GGG 0.646668 8_3:-35633984 None:intergenic
CATATTCGCAAAATTCTACA+CGG 0.662906 8_3:+35634613 Msa1286840:CDS
TAATTGGTAGAACCCATCAA+AGG 0.667309 8_3:-35634855 None:intergenic
ACGCGATTTAAGATACTGTG+TGG 0.685675 8_3:-35633937 None:intergenic
TTGACCCGACAATATTGGGG+AGG 0.695566 8_3:-35633981 None:intergenic
GCAGGCAGTGATGGAAACTG+AGG 0.718238 8_3:+35634180 Msa1286840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATAACTTCATTTTTTTT+GGG - chr8_3:35634775-35634794 None:intergenic 10.0%
!!! ATTAATAACTTCATTTTTTT+TGG - chr8_3:35634776-35634795 None:intergenic 10.0%
!!! TTAATAACTTCATTTTTTTT+GGG - chr8_3:35634775-35634794 None:intergenic 10.0%
!!! ATTAATAACTTCATTTTTTT+TGG - chr8_3:35634776-35634795 None:intergenic 10.0%
!! AATAGAAACACAATAAATTA+TGG - chr8_3:35634386-35634405 None:intergenic 15.0%
!! ATTTATTCATTAATAGAGTA+GGG - chr8_3:35634746-35634765 None:intergenic 15.0%
!!! ATTTATTTTCAATTTTCAGA+TGG + chr8_3:35634806-35634825 Msa1286840:intron 15.0%
!!! TTTATTTTCAATTTTCAGAT+GGG + chr8_3:35634807-35634826 Msa1286840:intron 15.0%
!! AATAGAAACACAATAAATTA+TGG - chr8_3:35634386-35634405 None:intergenic 15.0%
!! ATTTATTCATTAATAGAGTA+GGG - chr8_3:35634746-35634765 None:intergenic 15.0%
!!! ATTTATTTTCAATTTTCAGA+TGG + chr8_3:35634806-35634825 Msa1286840:intron 15.0%
!!! TTTATTTTCAATTTTCAGAT+GGG + chr8_3:35634807-35634826 Msa1286840:intron 15.0%
!! TTATATATTAGATGCAAACA+AGG + chr8_3:35634505-35634524 Msa1286840:intron 20.0%
!!! TCTGAAATCTAATAATATTC+TGG + chr8_3:35634639-35634658 Msa1286840:CDS 20.0%
!! CATTTATTCATTAATAGAGT+AGG - chr8_3:35634747-35634766 None:intergenic 20.0%
!! TTATATATTAGATGCAAACA+AGG + chr8_3:35634505-35634524 Msa1286840:intron 20.0%
!!! TCTGAAATCTAATAATATTC+TGG + chr8_3:35634639-35634658 Msa1286840:CDS 20.0%
!! CATTTATTCATTAATAGAGT+AGG - chr8_3:35634747-35634766 None:intergenic 20.0%
! CAAAATCAATAAATGTGACA+AGG - chr8_3:35634062-35634081 None:intergenic 25.0%
!!! TATCTTTGTTTTCAACATCA+TGG + chr8_3:35634358-35634377 Msa1286840:intron 25.0%
! ATATCTTATAGTGCAAGAAA+AGG + chr8_3:35634408-35634427 Msa1286840:intron 25.0%
! CAAAATCAATAAATGTGACA+AGG - chr8_3:35634062-35634081 None:intergenic 25.0%
!!! TATCTTTGTTTTCAACATCA+TGG + chr8_3:35634358-35634377 Msa1286840:intron 25.0%
! ATATCTTATAGTGCAAGAAA+AGG + chr8_3:35634408-35634427 Msa1286840:intron 25.0%
!!! TCATTGTTTTTGTGGTTTTC+TGG - chr8_3:35633913-35633932 None:intergenic 30.0%
!!! GTGGAAATTCATTGTTTTTG+TGG - chr8_3:35633921-35633940 None:intergenic 30.0%
! TTTGGAATGCTTAAAAACTC+CGG - chr8_3:35634265-35634284 None:intergenic 30.0%
!! AGTTTTTAAGCATTCCAAAC+TGG + chr8_3:35634266-35634285 Msa1286840:intron 30.0%
!!! TGACTTACTTTTTTCCAGTT+TGG - chr8_3:35634283-35634302 None:intergenic 30.0%
CATATTCGCAAAATTCTACA+CGG + chr8_3:35634613-35634632 Msa1286840:CDS 30.0%
!!! TCATTGTTTTTGTGGTTTTC+TGG - chr8_3:35633913-35633932 None:intergenic 30.0%
!!! GTGGAAATTCATTGTTTTTG+TGG - chr8_3:35633921-35633940 None:intergenic 30.0%
! TTTGGAATGCTTAAAAACTC+CGG - chr8_3:35634265-35634284 None:intergenic 30.0%
!! AGTTTTTAAGCATTCCAAAC+TGG + chr8_3:35634266-35634285 Msa1286840:intron 30.0%
!!! TGACTTACTTTTTTCCAGTT+TGG - chr8_3:35634283-35634302 None:intergenic 30.0%
CATATTCGCAAAATTCTACA+CGG + chr8_3:35634613-35634632 Msa1286840:CDS 30.0%
!! GTTTTTGTGGTTTTCTGGAA+TGG - chr8_3:35633908-35633927 None:intergenic 35.0%
AAGATTGACCCGACAATATT+GGG - chr8_3:35633988-35634007 None:intergenic 35.0%
AAAGATTGACCCGACAATAT+TGG - chr8_3:35633989-35634008 None:intergenic 35.0%
AATGCTTAAAAACTCCGGAT+TGG - chr8_3:35634260-35634279 None:intergenic 35.0%
! CGTGTAGAATTTTGCGAATA+TGG - chr8_3:35634615-35634634 None:intergenic 35.0%
! TCTGGCTTCAGTTGAAAAAT+TGG + chr8_3:35634657-35634676 Msa1286840:CDS 35.0%
AAGCTATCTTGACTTTAGTG+CGG + chr8_3:35634694-35634713 Msa1286840:intron 35.0%
TTAATAGAGTAGGGTGAAAG+AGG - chr8_3:35634737-35634756 None:intergenic 35.0%
TAATTGGTAGAACCCATCAA+AGG - chr8_3:35634858-35634877 None:intergenic 35.0%
ATCAACTGCAGTTGAGTAAT+TGG - chr8_3:35634874-35634893 None:intergenic 35.0%
!! GTTTTTGTGGTTTTCTGGAA+TGG - chr8_3:35633908-35633927 None:intergenic 35.0%
AAGATTGACCCGACAATATT+GGG - chr8_3:35633988-35634007 None:intergenic 35.0%
AAAGATTGACCCGACAATAT+TGG - chr8_3:35633989-35634008 None:intergenic 35.0%
AATGCTTAAAAACTCCGGAT+TGG - chr8_3:35634260-35634279 None:intergenic 35.0%
! CGTGTAGAATTTTGCGAATA+TGG - chr8_3:35634615-35634634 None:intergenic 35.0%
! TCTGGCTTCAGTTGAAAAAT+TGG + chr8_3:35634657-35634676 Msa1286840:CDS 35.0%
AAGCTATCTTGACTTTAGTG+CGG + chr8_3:35634694-35634713 Msa1286840:intron 35.0%
TTAATAGAGTAGGGTGAAAG+AGG - chr8_3:35634737-35634756 None:intergenic 35.0%
TAATTGGTAGAACCCATCAA+AGG - chr8_3:35634858-35634877 None:intergenic 35.0%
ATCAACTGCAGTTGAGTAAT+TGG - chr8_3:35634874-35634893 None:intergenic 35.0%
! ACGCGATTTAAGATACTGTG+TGG - chr8_3:35633940-35633959 None:intergenic 40.0%
CGTCTCTATGCTCAAAAACT+TGG + chr8_3:35633957-35633976 Msa1286840:CDS 40.0%
AGATTGACCCGACAATATTG+GGG - chr8_3:35633987-35634006 None:intergenic 40.0%
CTGCTATCTATTCAGATTGC+AGG + chr8_3:35634162-35634181 Msa1286840:intron 40.0%
GAAAAATCTGCAAGGACAAG+AGG - chr8_3:35634553-35634572 None:intergenic 40.0%
TTCACGCAGAAAAATCTGCA+AGG - chr8_3:35634561-35634580 None:intergenic 40.0%
! TAAAGCACCTGAACCTTTGA+TGG + chr8_3:35634842-35634861 Msa1286840:CDS 40.0%
! AAAGCACCTGAACCTTTGAT+GGG + chr8_3:35634843-35634862 Msa1286840:CDS 40.0%
! ACTCAACTGCAGTTGATATC+TGG + chr8_3:35634877-35634896 Msa1286840:CDS 40.0%
! TGCAGTTGATATCTGGTCTA+TGG + chr8_3:35634884-35634903 Msa1286840:CDS 40.0%
! GCAGTTGATATCTGGTCTAT+GGG + chr8_3:35634885-35634904 Msa1286840:CDS 40.0%
! GCATATTTGCTGAGATGCTT+AGG + chr8_3:35634910-35634929 Msa1286840:CDS 40.0%
! ACGCGATTTAAGATACTGTG+TGG - chr8_3:35633940-35633959 None:intergenic 40.0%
CGTCTCTATGCTCAAAAACT+TGG + chr8_3:35633957-35633976 Msa1286840:CDS 40.0%
AGATTGACCCGACAATATTG+GGG - chr8_3:35633987-35634006 None:intergenic 40.0%
CTGCTATCTATTCAGATTGC+AGG + chr8_3:35634162-35634181 Msa1286840:intron 40.0%
GAAAAATCTGCAAGGACAAG+AGG - chr8_3:35634553-35634572 None:intergenic 40.0%
TTCACGCAGAAAAATCTGCA+AGG - chr8_3:35634561-35634580 None:intergenic 40.0%
! TAAAGCACCTGAACCTTTGA+TGG + chr8_3:35634842-35634861 Msa1286840:CDS 40.0%
! AAAGCACCTGAACCTTTGAT+GGG + chr8_3:35634843-35634862 Msa1286840:CDS 40.0%
! ACTCAACTGCAGTTGATATC+TGG + chr8_3:35634877-35634896 Msa1286840:CDS 40.0%
! TGCAGTTGATATCTGGTCTA+TGG + chr8_3:35634884-35634903 Msa1286840:CDS 40.0%
! GCAGTTGATATCTGGTCTAT+GGG + chr8_3:35634885-35634904 Msa1286840:CDS 40.0%
! GCATATTTGCTGAGATGCTT+AGG + chr8_3:35634910-35634929 Msa1286840:CDS 40.0%
TTGGACCTCCCCAATATTGT+CGG + chr8_3:35633976-35633995 Msa1286840:CDS 45.0%
ATTCAGATTGCAGGCAGTGA+TGG + chr8_3:35634171-35634190 Msa1286840:intron 45.0%
CTGCAAGGACAAGAGGAAAA+CGG - chr8_3:35634546-35634565 None:intergenic 45.0%
ATAGAGTAGGGTGAAAGAGG+AGG - chr8_3:35634734-35634753 None:intergenic 45.0%
GGTTCAGGTGCTTTATAGCA+CGG - chr8_3:35634837-35634856 None:intergenic 45.0%
GTAGAACCCATCAAAGGTTC+AGG - chr8_3:35634852-35634871 None:intergenic 45.0%
TTAGGCTCGAACCTGTTCAT+TGG + chr8_3:35634928-35634947 Msa1286840:CDS 45.0%
TTGGACCTCCCCAATATTGT+CGG + chr8_3:35633976-35633995 Msa1286840:CDS 45.0%
ATTCAGATTGCAGGCAGTGA+TGG + chr8_3:35634171-35634190 Msa1286840:intron 45.0%
CTGCAAGGACAAGAGGAAAA+CGG - chr8_3:35634546-35634565 None:intergenic 45.0%
ATAGAGTAGGGTGAAAGAGG+AGG - chr8_3:35634734-35634753 None:intergenic 45.0%
GGTTCAGGTGCTTTATAGCA+CGG - chr8_3:35634837-35634856 None:intergenic 45.0%
GTAGAACCCATCAAAGGTTC+AGG - chr8_3:35634852-35634871 None:intergenic 45.0%
TTAGGCTCGAACCTGTTCAT+TGG + chr8_3:35634928-35634947 Msa1286840:CDS 45.0%
TGGACCTCCCCAATATTGTC+GGG + chr8_3:35633977-35633996 Msa1286840:CDS 50.0%
TTGACCCGACAATATTGGGG+AGG - chr8_3:35633984-35634003 None:intergenic 50.0%
! GCAGTGATGGAAACTGAGGA+TGG + chr8_3:35634184-35634203 Msa1286840:CDS 50.0%
TCGAAAACACGTCGCCAATC+CGG + chr8_3:35634243-35634262 Msa1286840:CDS 50.0%
TGAAGACGTGAAGCTCTCTC+TGG + chr8_3:35634577-35634596 Msa1286840:intron 50.0%
ACGTGAAGCTCTCTCTGGAT+TGG + chr8_3:35634582-35634601 Msa1286840:CDS 50.0%
TGGACCTCCCCAATATTGTC+GGG + chr8_3:35633977-35633996 Msa1286840:CDS 50.0%
TTGACCCGACAATATTGGGG+AGG - chr8_3:35633984-35634003 None:intergenic 50.0%
! GCAGTGATGGAAACTGAGGA+TGG + chr8_3:35634184-35634203 Msa1286840:CDS 50.0%
TCGAAAACACGTCGCCAATC+CGG + chr8_3:35634243-35634262 Msa1286840:CDS 50.0%
TGAAGACGTGAAGCTCTCTC+TGG + chr8_3:35634577-35634596 Msa1286840:intron 50.0%
ACGTGAAGCTCTCTCTGGAT+TGG + chr8_3:35634582-35634601 Msa1286840:CDS 50.0%
! GCAGGCAGTGATGGAAACTG+AGG + chr8_3:35634180-35634199 Msa1286840:CDS 55.0%
! GCAGGCAGTGATGGAAACTG+AGG + chr8_3:35634180-35634199 Msa1286840:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 35633895 35634953 35633895 ID=Msa1286840;Name=Msa1286840
chr8_3 mRNA 35633895 35634953 35633895 ID=Msa1286840-mRNA-1;Parent=Msa1286840;Name=Msa1286840-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0|0.75|0|0.25|4|0|143
chr8_3 exon 35633895 35633998 35633895 ID=Msa1286840-mRNA-1:exon:10505;Parent=Msa1286840-mRNA-1
chr8_3 exon 35634177 35634266 35634177 ID=Msa1286840-mRNA-1:exon:10506;Parent=Msa1286840-mRNA-1
chr8_3 exon 35634582 35634690 35634582 ID=Msa1286840-mRNA-1:exon:10507;Parent=Msa1286840-mRNA-1
chr8_3 exon 35634825 35634953 35634825 ID=Msa1286840-mRNA-1:exon:10508;Parent=Msa1286840-mRNA-1
chr8_3 CDS 35633895 35633998 35633895 ID=Msa1286840-mRNA-1:cds;Parent=Msa1286840-mRNA-1
chr8_3 CDS 35634177 35634266 35634177 ID=Msa1286840-mRNA-1:cds;Parent=Msa1286840-mRNA-1
chr8_3 CDS 35634582 35634690 35634582 ID=Msa1286840-mRNA-1:cds;Parent=Msa1286840-mRNA-1
chr8_3 CDS 35634825 35634953 35634825 ID=Msa1286840-mRNA-1:cds;Parent=Msa1286840-mRNA-1
Gene Sequence

>Msa1286840

ATGGTTTTAACCATTCCAGAAAACCACAAAAACAATGAATTTCCACACAGTATCTTAAATCGCGTCTCTATGCTCAAAAACTTGGACCTCCCCAATATTGTCGGATTGCAGGCAGTGATGGAAACTGAGGATGGAGTGTTTCTTGTTTATGAGCATTTTCAAACTAGTCTTCGAAAACACGTCGCCAATCCGGAACGTGAAGCTCTCTCTGGATTGGCTTTCTGCCATATTCGCAAAATTCTACACGGAGATCTGAAATCTAATAATATTCTGGCTTCAGTTGAAAAATTGGATGTCAAGAAAATGGGTAATCCGTGCTATAAAGCACCTGAACCTTTGATGGGTTCTACCAATTACTCAACTGCAGTTGATATCTGGTCTATGGGATGCATATTTGCTGAGATGCTTAGGCTCGAACCTGTTCATTGGTAA
Protein sequence

>Msa1286840

MVLTIPENHKNNEFPHSILNRVSMLKNLDLPNIVGLQAVMETEDGVFLVYEHFQTSLRKHVANPEREALSGLAFCHIRKILHGDLKSNNILASVEKLDVKKMGNPCYKAPEPLMGSTNYSTAVDIWSMGCIFAEMLRLEPVHW*