AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1287110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1287110 MtrunA17_Chr8g0360761 72.180 133 16 2 1 127 75 192 1.88e-57 176
Msa1287110 MtrunA17_Chr4g0051261 51.240 121 52 2 6 120 169 288 2.25e-33 118
Msa1287110 MtrunA17_Chr4g0002001 50.833 120 51 2 6 119 186 303 1.09e-31 114
Msa1287110 MtrunA17_Chr2g0293491 48.760 121 55 2 6 120 169 288 4.94e-31 112
Msa1287110 MtrunA17_Chr1g0187371 47.500 120 55 2 6 119 191 308 8.83e-31 111
Msa1287110 MtrunA17_Chr8g0358921 45.833 120 58 2 6 119 217 335 5.72e-28 104
Msa1287110 MtrunA17_Chr4g0057791 41.406 128 62 2 6 120 385 512 2.95e-24 96.3
Msa1287110 MtrunA17_Chr8g0377071 42.969 128 60 3 6 120 550 677 1.27e-23 94.7
Msa1287110 MtrunA17_Chr3g0122781 60.606 66 26 0 6 71 581 646 1.52e-22 91.7
Msa1287110 MtrunA17_Chr3g0093391 42.623 122 60 3 6 119 203 322 2.79e-22 90.9
Msa1287110 MtrunA17_Chr6g0486031 40.476 126 68 2 1 119 275 400 8.16e-22 89.0
Msa1287110 MtrunA17_Chr4g0030231 41.129 124 66 2 6 122 206 329 1.16e-21 88.2
Msa1287110 MtrunA17_Chr3g0093571 42.149 121 62 3 6 119 205 324 2.47e-21 88.2
Msa1287110 MtrunA17_Chr4g0045841 36.220 127 73 2 6 126 221 345 2.83e-21 87.4
Msa1287110 MtrunA17_Chr4g0030151 38.281 128 70 3 6 126 205 330 3.68e-20 84.3
Msa1287110 MtrunA17_Chr5g0397261 39.231 130 70 4 6 127 207 335 1.68e-19 82.4
Msa1287110 MtrunA17_Chr3g0102571 35.833 120 71 1 6 119 247 366 1.97e-19 82.4
Msa1287110 MtrunA17_Chr7g0248461 38.017 121 68 2 6 119 204 324 9.94e-19 80.1
Msa1287110 MtrunA17_Chr8g0387401 38.400 125 68 4 6 122 218 341 3.05e-18 79.0
Msa1287110 MtrunA17_Chr3g0130781 34.058 138 66 4 6 121 196 330 4.44e-18 79.0
Msa1287110 MtrunA17_Chr8g0390341 36.508 126 73 2 1 119 288 413 1.98e-17 77.0
Msa1287110 MtrunA17_Chr8g0369451 35.537 121 72 3 6 120 164 284 2.12e-17 76.6
Msa1287110 MtrunA17_Chr7g0218071 53.333 60 28 0 6 65 286 345 2.40e-17 76.6
Msa1287110 MtrunA17_Chr6g0453711 51.667 60 29 0 6 65 276 335 5.29e-17 75.9
Msa1287110 MtrunA17_Chr6g0483151 36.667 120 68 3 6 119 177 294 5.44e-17 75.5
Msa1287110 MtrunA17_Chr4g0061141 35.537 121 71 3 6 120 271 390 8.54e-17 75.1
Msa1287110 MtrunA17_Chr3g0099951 33.898 118 74 2 6 119 179 296 1.61e-16 74.3
Msa1287110 MtrunA17_Chr1g0201571 50.000 60 30 0 6 65 292 351 3.35e-16 73.6
Msa1287110 MtrunA17_Chr2g0277011 38.843 121 65 3 6 119 256 374 6.15e-16 72.8
Msa1287110 MtrunA17_Chr8g0348481 34.711 121 72 2 6 119 203 323 6.43e-16 72.4
Msa1287110 MtrunA17_Chr5g0396361 50.000 60 30 0 6 65 306 365 6.74e-16 72.8
Msa1287110 MtrunA17_Chr8g0378831 34.426 122 71 3 6 119 199 319 8.74e-16 72.0
Msa1287110 MtrunA17_Chr4g0030241 55.172 58 26 0 6 63 67 124 9.66e-16 69.7
Msa1287110 MtrunA17_Chr1g0193081 35.385 130 57 3 6 119 241 359 1.45e-15 71.6
Msa1287110 MtrunA17_Chr7g0246591 37.500 120 68 3 6 119 307 425 1.97e-15 71.2
Msa1287110 MtrunA17_Chr7g0270371 39.167 120 64 3 6 118 241 358 3.58e-15 70.5
Msa1287110 MtrunA17_Chr5g0409421 33.884 121 74 2 6 120 164 284 4.22e-15 70.5
Msa1287110 MtrunA17_Chr8g0385971 51.667 60 29 0 6 65 256 315 4.26e-15 70.5
Msa1287110 MtrunA17_Chr2g0320011 34.615 130 58 3 6 119 254 372 4.54e-15 70.1
Msa1287110 MtrunA17_Chr2g0317501 50.000 60 30 0 6 65 300 359 6.00e-15 70.1
Msa1287110 MtrunA17_Chr4g0066691 48.333 60 31 0 6 65 270 329 6.40e-15 69.7
Msa1287110 MtrunA17_Chr3g0102601 31.933 119 75 1 7 119 86 204 8.02e-15 68.6
Msa1287110 MtrunA17_Chr1g0194201 38.017 121 66 3 6 119 241 359 8.07e-15 69.3
Msa1287110 MtrunA17_Chr2g0286421 35.156 128 60 6 6 119 268 386 8.53e-15 69.3
Msa1287110 MtrunA17_Chr3g0141871 35.484 124 67 2 6 119 199 319 8.75e-15 69.3
Msa1287110 MtrunA17_Chr2g0284091 51.724 58 28 0 6 63 206 263 2.12e-14 68.2
Msa1287110 MtrunA17_Chr3g0131861 31.783 129 63 3 6 119 263 381 2.20e-14 68.2
Msa1287110 MtrunA17_Chr8g0392811 36.567 134 67 6 6 126 287 415 3.11e-14 67.8
Msa1287110 MtrunA17_Chr8g0339221 32.331 133 67 4 6 123 164 288 3.15e-14 67.8
Msa1287110 MtrunA17_Chr1g0191221 45.000 60 33 0 6 65 288 347 1.66e-13 65.9
Msa1287110 MtrunA17_Chr4g0040141 32.231 121 73 3 6 119 295 413 1.76e-13 65.9
Msa1287110 MtrunA17_Chr8g0350311 48.276 58 30 0 6 63 305 362 2.02e-13 65.5
Msa1287110 MtrunA17_Chr2g0324461 42.105 76 42 1 6 81 307 380 2.11e-13 65.5
Msa1287110 MtrunA17_Chr7g0273601 43.333 60 34 0 6 65 273 332 3.07e-13 65.1
Msa1287110 MtrunA17_Chr8g0359091 36.220 127 69 4 6 122 196 320 8.03e-13 63.9
Msa1287110 MtrunA17_Chr8g0383451 46.552 58 31 0 6 63 164 221 1.12e-12 63.5
Msa1287110 MtrunA17_Chr4g0065851 36.923 130 58 7 6 119 195 316 1.34e-12 63.2
Msa1287110 MtrunA17_Chr6g0482681 37.226 137 62 7 6 126 259 387 1.45e-12 63.2
Msa1287110 MtrunA17_Chr5g0443351 37.692 130 57 7 6 119 192 313 1.81e-12 62.8
Msa1287110 MtrunA17_Chr8g0384301 32.231 121 56 4 2 120 460 556 8.25e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1287110 AT2G38620.4 50.833 120 51 2 6 119 124 241 1.74e-32 115
Msa1287110 AT2G38620.2 50.833 120 51 2 6 119 186 303 6.50e-32 115
Msa1287110 AT1G76540.1 48.333 120 54 2 6 119 187 304 1.43e-31 114
Msa1287110 AT3G54180.1 50.000 120 52 2 6 119 184 301 2.54e-31 113
Msa1287110 AT1G20930.1 47.500 120 55 2 6 119 189 306 3.54e-31 113
Msa1287110 AT3G48750.1 46.341 123 59 2 6 122 169 290 6.28e-29 106
Msa1287110 AT5G63370.7 41.085 129 59 3 6 119 467 593 4.53e-24 96.3
Msa1287110 AT5G63370.8 41.085 129 59 3 6 119 467 593 4.53e-24 96.3
Msa1287110 AT5G63370.4 41.085 129 59 3 6 119 467 593 4.53e-24 96.3
Msa1287110 AT5G63370.6 41.085 129 59 3 6 119 467 593 4.53e-24 96.3
Msa1287110 AT5G63370.1 41.085 129 59 3 6 119 467 593 4.53e-24 96.3
Msa1287110 AT3G59790.1 40.000 120 66 1 6 119 226 345 2.05e-23 93.6
Msa1287110 AT5G63370.2 41.085 129 59 3 6 119 333 459 3.19e-23 93.6
Msa1287110 AT5G63370.3 41.085 129 59 3 6 119 333 459 3.19e-23 93.6
Msa1287110 AT1G67580.1 42.188 128 61 3 6 120 571 698 6.04e-23 93.2
Msa1287110 AT1G67580.2 42.188 128 61 3 6 120 571 698 6.04e-23 93.2
Msa1287110 AT5G63370.5 56.164 73 32 0 6 78 467 539 6.37e-22 90.1
Msa1287110 AT5G63370.10 56.164 73 32 0 6 78 467 539 6.37e-22 90.1
Msa1287110 AT5G10270.1 42.975 121 61 3 6 119 206 325 8.68e-22 89.7
Msa1287110 AT1G07880.2 40.984 122 63 3 6 119 199 319 2.51e-21 87.8
Msa1287110 AT5G63370.9 56.164 73 32 0 6 78 333 405 3.42e-21 87.8
Msa1287110 AT1G01560.2 40.496 121 65 2 6 119 206 326 3.97e-20 84.3
Msa1287110 AT1G18040.1 37.190 121 66 3 6 119 175 292 4.10e-20 84.7
Msa1287110 AT1G73690.1 38.983 118 68 3 6 119 174 291 1.47e-19 83.2
Msa1287110 AT2G43790.1 37.008 127 72 2 6 126 229 353 2.69e-19 82.4
Msa1287110 AT3G45640.1 38.281 128 70 3 6 126 204 329 3.87e-19 81.6
Msa1287110 AT4G11330.1 37.209 129 74 2 6 127 209 337 6.62e-19 81.3
Msa1287110 AT2G46070.1 39.669 121 66 2 6 119 207 327 1.24e-18 80.5
Msa1287110 AT3G01085.1 39.669 121 65 3 6 119 281 400 1.83e-18 80.5
Msa1287110 AT3G01085.2 39.669 121 65 3 6 119 281 400 1.83e-18 80.5
Msa1287110 AT3G01085.3 39.669 121 65 3 6 119 281 400 1.83e-18 80.5
Msa1287110 AT4G01370.1 38.843 121 67 2 6 119 209 329 2.36e-18 79.7
Msa1287110 AT5G63610.1 37.778 135 65 4 6 121 201 335 2.94e-18 79.7
Msa1287110 AT5G64960.1 37.097 124 64 2 6 119 206 325 3.95e-18 79.3
Msa1287110 AT2G46070.3 39.669 121 66 2 6 119 241 361 4.30e-18 79.0
Msa1287110 AT5G64960.2 37.097 124 64 2 6 119 153 272 4.81e-18 79.0
Msa1287110 AT4G19110.2 36.364 121 71 3 6 120 164 284 6.49e-18 78.6
Msa1287110 AT4G19110.1 36.364 121 71 3 6 120 164 284 8.34e-18 78.6
Msa1287110 AT4G28980.3 35.714 126 74 2 1 119 293 418 2.31e-17 77.0
Msa1287110 AT4G28980.1 35.714 126 74 2 1 119 293 418 2.31e-17 77.0
Msa1287110 AT4G28980.2 35.714 126 74 2 1 119 293 418 2.31e-17 77.0
Msa1287110 AT4G19110.3 36.364 121 71 3 6 120 108 228 2.43e-17 77.0
Msa1287110 AT2G38620.3 65.385 52 18 0 6 57 186 237 3.98e-17 75.1
Msa1287110 AT2G38620.1 65.385 52 18 0 6 57 186 237 3.98e-17 75.1
Msa1287110 AT1G66750.1 35.833 120 69 2 6 119 176 293 5.85e-17 75.5
Msa1287110 AT1G57870.3 40.164 122 62 4 6 119 248 366 1.10e-16 75.1
Msa1287110 AT1G57870.1 40.164 122 62 4 6 119 248 366 1.13e-16 75.1
Msa1287110 AT1G57870.4 40.164 122 62 4 6 119 248 366 1.13e-16 75.1
Msa1287110 AT1G57870.2 40.164 122 62 4 6 119 248 366 1.13e-16 75.1
Msa1287110 AT1G33770.1 50.000 60 30 0 6 65 307 366 3.95e-16 73.6
Msa1287110 AT1G10210.2 36.364 121 70 2 6 119 199 319 3.96e-16 73.6
Msa1287110 AT1G10210.3 36.364 121 70 2 6 119 199 319 3.96e-16 73.6
Msa1287110 AT1G10210.1 36.364 121 70 2 6 119 199 319 3.96e-16 73.6
Msa1287110 AT4G13020.5 36.290 124 73 2 6 123 173 296 4.34e-16 73.6
Msa1287110 AT1G09840.3 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.7 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.10 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.6 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.4 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.1 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.9 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.5 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.8 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT1G09840.2 40.000 120 61 4 6 117 249 365 4.35e-16 73.6
Msa1287110 AT4G13020.2 36.290 124 73 2 6 123 172 295 4.42e-16 73.6
Msa1287110 AT4G13020.3 36.290 124 73 2 6 123 172 295 4.42e-16 73.6
Msa1287110 AT1G53050.2 38.017 121 66 3 6 119 300 418 4.55e-16 73.6
Msa1287110 AT1G53050.1 38.017 121 66 3 6 119 300 418 4.55e-16 73.6
Msa1287110 AT4G13020.6 36.290 124 73 2 6 123 164 287 4.62e-16 73.2
Msa1287110 AT4G13020.4 36.290 124 73 2 6 123 164 287 4.64e-16 73.2
Msa1287110 AT4G13020.7 36.290 124 73 2 6 123 164 287 4.70e-16 73.2
Msa1287110 AT4G13020.1 36.290 124 73 2 6 123 164 287 4.82e-16 73.2
Msa1287110 AT4G13020.8 36.290 124 73 2 6 123 173 296 4.95e-16 73.2
Msa1287110 AT5G39420.1 50.000 60 30 0 6 65 271 330 5.12e-16 73.6
Msa1287110 AT1G59580.2 38.017 121 68 4 6 119 199 319 1.03e-15 72.4
Msa1287110 AT1G59580.1 38.017 121 68 4 6 119 199 319 1.03e-15 72.4
Msa1287110 AT1G01560.3 38.136 118 64 3 6 114 206 323 1.48e-15 71.6
Msa1287110 AT1G74330.3 48.333 60 31 0 6 65 289 348 1.64e-15 72.0
Msa1287110 AT1G74330.2 48.333 60 31 0 6 65 289 348 1.65e-15 72.0
Msa1287110 AT1G74330.1 48.333 60 31 0 6 65 289 348 1.65e-15 72.0
Msa1287110 AT5G45430.1 34.711 121 73 3 6 120 164 284 1.78e-15 71.6
Msa1287110 AT5G44290.2 37.903 124 64 5 6 120 303 422 1.91e-15 71.6
Msa1287110 AT5G44290.5 37.903 124 64 5 6 120 303 422 1.91e-15 71.6
Msa1287110 AT5G44290.4 37.903 124 64 5 6 120 303 422 1.91e-15 71.6
Msa1287110 AT5G44290.1 37.903 124 64 5 6 120 303 422 1.91e-15 71.6
Msa1287110 AT5G44290.3 37.903 124 64 5 6 120 303 422 1.91e-15 71.6
Msa1287110 AT5G44290.6 37.903 124 64 5 6 120 303 422 2.02e-15 71.6
Msa1287110 AT5G45430.2 34.711 121 73 3 6 120 164 284 2.04e-15 71.6
Msa1287110 AT1G57700.1 50.000 60 30 0 6 65 312 371 2.15e-15 71.6
Msa1287110 AT1G09600.1 50.000 60 30 0 6 65 329 388 2.59e-15 71.2
Msa1287110 AT1G09840.11 39.496 119 63 3 6 117 122 238 2.89e-15 70.5
Msa1287110 AT1G18670.1 48.333 60 31 0 6 65 298 357 4.37e-15 70.9
Msa1287110 AT1G18670.3 48.333 60 31 0 6 65 298 357 4.37e-15 70.9
Msa1287110 AT1G03740.2 35.433 127 63 5 6 120 379 498 4.94e-15 70.5
Msa1287110 AT1G18670.2 48.333 60 31 0 6 65 298 357 5.01e-15 70.5
Msa1287110 AT5G44290.7 37.903 124 64 5 6 120 303 422 5.06e-15 70.5
Msa1287110 AT1G03740.1 35.433 127 63 5 6 120 379 498 5.53e-15 70.5
Msa1287110 AT3G61160.2 34.400 125 65 3 6 119 275 393 8.18e-15 69.7
Msa1287110 AT5G14640.1 38.017 121 66 3 6 119 240 358 8.36e-15 69.7
Msa1287110 AT5G14640.2 38.017 121 66 3 6 119 240 358 8.36e-15 69.7
Msa1287110 AT3G61160.1 34.400 125 65 3 6 119 268 386 1.16e-14 69.3
Msa1287110 AT3G05840.2 37.190 121 67 3 6 119 239 357 1.18e-14 69.3
Msa1287110 AT3G05840.1 37.190 121 67 3 6 119 239 357 1.18e-14 69.3
Msa1287110 AT3G61160.4 34.400 125 65 3 6 119 268 386 1.18e-14 69.3
Msa1287110 AT1G54610.1 46.667 60 32 0 6 65 284 343 1.25e-14 69.3
Msa1287110 AT1G54610.3 46.667 60 32 0 6 65 284 343 1.25e-14 69.3
Msa1287110 AT1G54610.2 46.667 60 32 0 6 65 284 343 1.25e-14 69.3
Msa1287110 AT1G71530.1 33.884 121 71 3 6 119 313 431 2.34e-14 68.6
Msa1287110 AT5G26751.1 37.190 121 67 3 6 119 235 353 2.50e-14 68.6
Msa1287110 AT4G18710.1 51.724 58 28 0 6 63 206 263 2.99e-14 68.2
Msa1287110 AT5G50860.1 48.333 60 31 0 6 65 280 339 3.84e-14 68.2
Msa1287110 AT5G50860.2 48.333 60 31 0 6 65 280 339 4.82e-14 67.8
Msa1287110 AT4G00720.1 37.190 121 67 3 6 119 304 422 6.51e-14 67.4
Msa1287110 AT1G71530.3 35.345 116 66 3 6 114 313 426 6.86e-14 67.4
Msa1287110 AT2G30980.1 50.000 58 29 0 6 63 238 295 7.32e-14 67.0
Msa1287110 AT2G46070.2 36.937 111 63 2 6 109 207 317 9.57e-14 66.6
Msa1287110 AT4G18710.2 50.000 58 29 0 6 63 200 257 9.86e-14 66.2
Msa1287110 AT1G06390.2 50.000 58 29 0 6 63 236 293 1.03e-13 66.6
Msa1287110 AT1G06390.1 50.000 58 29 0 6 63 236 293 1.03e-13 66.6
Msa1287110 AT1G71530.2 33.884 121 71 3 6 119 313 431 1.14e-13 66.6
Msa1287110 AT4G22940.2 30.400 125 72 4 6 120 270 389 3.25e-13 65.5
Msa1287110 AT4G22940.1 30.400 125 72 4 6 120 270 389 5.46e-13 64.7
Msa1287110 AT2G18170.1 33.607 122 72 3 6 119 199 319 6.20e-13 64.3
Msa1287110 AT3G05050.3 44.444 63 35 0 6 68 304 366 7.33e-13 64.3
Msa1287110 AT3G05050.2 44.444 63 35 0 6 68 304 366 7.33e-13 64.3
Msa1287110 AT3G05050.1 44.444 63 35 0 6 68 304 366 7.33e-13 64.3
Msa1287110 AT4G36450.1 33.065 124 70 3 6 119 196 316 8.96e-13 63.9
Msa1287110 AT3G61160.5 46.552 58 31 0 6 63 268 325 1.01e-12 63.9
Msa1287110 AT3G61160.6 46.552 58 31 0 6 63 275 332 1.04e-12 63.5
Msa1287110 AT3G61160.3 46.552 58 31 0 6 63 268 325 1.07e-12 63.5
Msa1287110 AT3G14720.2 38.462 130 62 6 6 122 195 319 1.21e-12 63.5
Msa1287110 AT3G14720.3 38.462 130 62 6 6 122 195 319 1.21e-12 63.5
Msa1287110 AT1G73670.1 36.296 135 62 7 6 124 260 386 1.33e-12 63.5
Msa1287110 AT2G01450.3 35.075 134 69 6 6 126 186 314 1.41e-12 63.5
Msa1287110 AT2G01450.1 35.075 134 69 6 6 126 186 314 1.41e-12 63.5
Msa1287110 AT2G01450.2 35.075 134 69 6 6 126 186 314 1.41e-12 63.5
Msa1287110 AT2G01450.4 35.075 134 69 6 6 126 186 314 1.41e-12 63.5
Msa1287110 AT3G18040.3 37.778 135 64 6 6 126 302 430 1.64e-12 63.5
Msa1287110 AT4G10010.1 40.000 60 36 0 6 65 142 201 1.67e-12 63.2
Msa1287110 AT4G10010.2 40.000 60 36 0 6 65 322 381 1.76e-12 63.2
Msa1287110 AT3G18040.4 37.778 135 64 6 6 126 302 430 1.76e-12 63.2
Msa1287110 AT1G07880.1 58.333 48 20 0 6 53 199 246 2.06e-12 62.4
Msa1287110 AT3G18040.5 37.778 135 64 6 6 126 188 316 3.28e-12 62.4
Msa1287110 AT3G18040.1 37.778 135 64 6 6 126 193 321 3.28e-12 62.4
Msa1287110 AT2G42880.1 35.878 131 64 6 6 122 195 319 3.89e-12 62.4
Msa1287110 AT3G14720.1 38.462 130 62 6 6 122 195 319 4.24e-12 62.0
Msa1287110 AT2G42880.2 35.878 131 64 6 6 122 195 319 5.19e-12 62.0
Msa1287110 AT3G18040.2 37.778 135 64 6 6 126 105 233 1.06e-11 60.8
Msa1287110 AT1G18150.3 35.766 137 64 7 6 126 274 402 3.81e-11 59.3
Msa1287110 AT1G18150.2 35.766 137 64 7 6 126 274 402 3.81e-11 59.3
Msa1287110 AT1G18150.1 35.766 137 64 7 6 126 274 402 3.81e-11 59.3
Msa1287110 AT1G53510.1 35.433 127 72 4 6 123 195 320 4.97e-11 59.3

Find 32 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTGAAATCCTGCTATT+TGG 0.134409 8_3:+36844345 None:intergenic
AGATATTGGCTGCGATATTT+TGG 0.233067 8_3:-36844899 Msa1287110:intron
TGCAGTTGATATCTGGTCTT+TGG 0.256878 8_3:-36844982 Msa1287110:CDS
ATCAACTGCAGTTGAGTAAT+TGG 0.289792 8_3:+36844995 None:intergenic
ATGAATACCTTTGGAACAAT+TGG 0.311260 8_3:+36844706 None:intergenic
CAGCTGAAATCCTGCTATTT+GGG 0.318029 8_3:+36844346 None:intergenic
ACTCAACTGCAGTTGATATC+TGG 0.396574 8_3:-36844989 Msa1287110:CDS
TAATGATGAACCGCAGATAT+TGG 0.411947 8_3:-36844913 Msa1287110:CDS
GCATATTTGCTGAGATGCTT+AGG 0.419785 8_3:-36844956 Msa1287110:CDS
AGGCTCGAACCTCTGTTCAT+TGG 0.422138 8_3:-36844936 Msa1287110:CDS
GCAGTTGATATCTGGTCTTT+GGG 0.432779 8_3:-36844981 Msa1287110:CDS
AAGACCATCTGTTCGAGCTC+AGG 0.436151 8_3:+36844586 None:intergenic
GTGGTTAAAATTCGAAGATA+TGG 0.441943 8_3:+36844306 None:intergenic
GTACGCCAACTGAAGAAATC+TGG 0.446682 8_3:-36844774 Msa1287110:CDS
GTAGAACCCATCAAAAGTTC+AGG 0.451436 8_3:+36845017 None:intergenic
TTTGCCTGTGCCCAAATAGC+AGG 0.491915 8_3:-36844356 Msa1287110:CDS
AATACCTTTGGAACAATTGG+AGG 0.500345 8_3:+36844709 None:intergenic
AGGAGCTTCATCAATCATCA+TGG 0.514486 8_3:+36844283 None:intergenic
CCAGGCCAGATTTCTTCAGT+TGG 0.518287 8_3:+36844769 None:intergenic
AGACCATCTGTTCGAGCTCA+GGG 0.521043 8_3:+36844587 None:intergenic
AGTTCAGGTGCTTTATAGCA+CGG 0.527126 8_3:+36845032 None:intergenic
CAACCCTCCAATTGTTCCAA+AGG 0.531079 8_3:-36844713 Msa1287110:intron
AAATCCTGCTATTTGGGCAC+AGG 0.541827 8_3:+36844352 None:intergenic
ATTCCCTGAGCTCGAACAGA+TGG 0.548425 8_3:-36844590 Msa1287110:intron
CCAACTGAAGAAATCTGGCC+TGG 0.559077 8_3:-36844769 Msa1287110:CDS
GCTCAGGGAATTCTGCTGCT+AGG 0.577996 8_3:+36844602 None:intergenic
GGAGCTTCATCAATCATCAT+GGG 0.611673 8_3:+36844284 None:intergenic
ATACCTTTGGAACAATTGGA+GGG 0.612490 8_3:+36844710 None:intergenic
GCTTCATCAATCATCATGGG+TGG 0.679817 8_3:+36844287 None:intergenic
TTGCATATAGAAGACACACC+AGG 0.683383 8_3:+36844751 None:intergenic
TCATCATTACCAATGAACAG+AGG 0.709071 8_3:+36844927 None:intergenic
AATATCGCAGCCAATATCTG+CGG 0.714594 8_3:+36844903 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATTGCTTATTTATAAA+TGG + chr8_3:36844686-36844705 None:intergenic 10.0%
!! TAATATTGCTTATTTATAAA+TGG + chr8_3:36844686-36844705 None:intergenic 10.0%
!! AGAAATCATAAAAATAGTTA+TGG + chr8_3:36844477-36844496 None:intergenic 15.0%
!! AGAAATCATAAAAATAGTTA+TGG + chr8_3:36844477-36844496 None:intergenic 15.0%
!! TGCTTATTTATAAATGGTAA+TGG + chr8_3:36844680-36844699 None:intergenic 20.0%
!! CAATATTATATGCAAACTAT+TGG - chr8_3:36844698-36844717 Msa1287110:intron 20.0%
!! AATTAATTCTCAAAGATATG+AGG + chr8_3:36844798-36844817 None:intergenic 20.0%
!! TTTAAGCAGATTGAAAATAT+AGG - chr8_3:36844886-36844905 Msa1287110:intron 20.0%
!! ATCTAGTGAATGTTTATTAA+TGG - chr8_3:36844927-36844946 Msa1287110:CDS 20.0%
!! TGCTTATTTATAAATGGTAA+TGG + chr8_3:36844680-36844699 None:intergenic 20.0%
!! CAATATTATATGCAAACTAT+TGG - chr8_3:36844698-36844717 Msa1287110:intron 20.0%
!! AATTAATTCTCAAAGATATG+AGG + chr8_3:36844798-36844817 None:intergenic 20.0%
!! TTTAAGCAGATTGAAAATAT+AGG - chr8_3:36844886-36844905 Msa1287110:intron 20.0%
!! ATCTAGTGAATGTTTATTAA+TGG - chr8_3:36844927-36844946 Msa1287110:CDS 20.0%
!!! TTTTATATGTAAAGCTTGCT+TGG - chr8_3:36844538-36844557 Msa1287110:intron 25.0%
! ATAAACATTCACTAGATCAA+TGG + chr8_3:36844924-36844943 None:intergenic 25.0%
!!! TTTTATATGTAAAGCTTGCT+TGG - chr8_3:36844538-36844557 Msa1287110:intron 25.0%
! ATAAACATTCACTAGATCAA+TGG + chr8_3:36844924-36844943 None:intergenic 25.0%
!! ATGAATACCTTTGGAACAAT+TGG + chr8_3:36844631-36844650 None:intergenic 30.0%
ATGTAGAACATGAATACCTT+TGG + chr8_3:36844640-36844659 None:intergenic 30.0%
! TGCAAACTATTGGATTTTGT+AGG - chr8_3:36844708-36844727 Msa1287110:intron 30.0%
! GCAAACTATTGGATTTTGTA+GGG - chr8_3:36844709-36844728 Msa1287110:intron 30.0%
GTGGTTAAAATTCGAAGATA+TGG + chr8_3:36845031-36845050 None:intergenic 30.0%
!! ATGAATACCTTTGGAACAAT+TGG + chr8_3:36844631-36844650 None:intergenic 30.0%
ATGTAGAACATGAATACCTT+TGG + chr8_3:36844640-36844659 None:intergenic 30.0%
! TGCAAACTATTGGATTTTGT+AGG - chr8_3:36844708-36844727 Msa1287110:intron 30.0%
! GCAAACTATTGGATTTTGTA+GGG - chr8_3:36844709-36844728 Msa1287110:intron 30.0%
GTGGTTAAAATTCGAAGATA+TGG + chr8_3:36845031-36845050 None:intergenic 30.0%
!! TAAAGCACCTGAACTTTTGA+TGG - chr8_3:36844310-36844329 Msa1287110:CDS 35.0%
!! AAAGCACCTGAACTTTTGAT+GGG - chr8_3:36844311-36844330 Msa1287110:CDS 35.0%
ATCAACTGCAGTTGAGTAAT+TGG + chr8_3:36844342-36844361 None:intergenic 35.0%
TCATCATTACCAATGAACAG+AGG + chr8_3:36844410-36844429 None:intergenic 35.0%
TAATGATGAACCGCAGATAT+TGG - chr8_3:36844421-36844440 Msa1287110:intron 35.0%
!! AGATATTGGCTGCGATATTT+TGG - chr8_3:36844435-36844454 Msa1287110:intron 35.0%
!! ATACCTTTGGAACAATTGGA+GGG + chr8_3:36844627-36844646 None:intergenic 35.0%
!! AATACCTTTGGAACAATTGG+AGG + chr8_3:36844628-36844647 None:intergenic 35.0%
! AGATGGTCTTGATCTTCTTT+CGG - chr8_3:36844761-36844780 Msa1287110:CDS 35.0%
! TAATGGCAAGTGTTTTGTGT+TGG - chr8_3:36844944-36844963 Msa1287110:CDS 35.0%
!! TAAAGCACCTGAACTTTTGA+TGG - chr8_3:36844310-36844329 Msa1287110:CDS 35.0%
!! AAAGCACCTGAACTTTTGAT+GGG - chr8_3:36844311-36844330 Msa1287110:CDS 35.0%
ATCAACTGCAGTTGAGTAAT+TGG + chr8_3:36844342-36844361 None:intergenic 35.0%
TCATCATTACCAATGAACAG+AGG + chr8_3:36844410-36844429 None:intergenic 35.0%
TAATGATGAACCGCAGATAT+TGG - chr8_3:36844421-36844440 Msa1287110:intron 35.0%
!! AGATATTGGCTGCGATATTT+TGG - chr8_3:36844435-36844454 Msa1287110:intron 35.0%
!! ATACCTTTGGAACAATTGGA+GGG + chr8_3:36844627-36844646 None:intergenic 35.0%
!! AATACCTTTGGAACAATTGG+AGG + chr8_3:36844628-36844647 None:intergenic 35.0%
! AGATGGTCTTGATCTTCTTT+CGG - chr8_3:36844761-36844780 Msa1287110:CDS 35.0%
! TAATGGCAAGTGTTTTGTGT+TGG - chr8_3:36844944-36844963 Msa1287110:CDS 35.0%
AGTTCAGGTGCTTTATAGCA+CGG + chr8_3:36844305-36844324 None:intergenic 40.0%
GTAGAACCCATCAAAAGTTC+AGG + chr8_3:36844320-36844339 None:intergenic 40.0%
! ACTCAACTGCAGTTGATATC+TGG - chr8_3:36844345-36844364 Msa1287110:CDS 40.0%
! TGCAGTTGATATCTGGTCTT+TGG - chr8_3:36844352-36844371 Msa1287110:CDS 40.0%
! GCAGTTGATATCTGGTCTTT+GGG - chr8_3:36844353-36844372 Msa1287110:CDS 40.0%
! GCATATTTGCTGAGATGCTT+AGG - chr8_3:36844378-36844397 Msa1287110:intron 40.0%
AATATCGCAGCCAATATCTG+CGG + chr8_3:36844434-36844453 None:intergenic 40.0%
TTGCATATAGAAGACACACC+AGG + chr8_3:36844586-36844605 None:intergenic 40.0%
! CAGCTGAAATCCTGCTATTT+GGG + chr8_3:36844991-36845010 None:intergenic 40.0%
TCAGCTGAAATCCTGCTATT+TGG + chr8_3:36844992-36845011 None:intergenic 40.0%
AGTTCAGGTGCTTTATAGCA+CGG + chr8_3:36844305-36844324 None:intergenic 40.0%
GTAGAACCCATCAAAAGTTC+AGG + chr8_3:36844320-36844339 None:intergenic 40.0%
! ACTCAACTGCAGTTGATATC+TGG - chr8_3:36844345-36844364 Msa1287110:CDS 40.0%
! TGCAGTTGATATCTGGTCTT+TGG - chr8_3:36844352-36844371 Msa1287110:CDS 40.0%
! GCAGTTGATATCTGGTCTTT+GGG - chr8_3:36844353-36844372 Msa1287110:CDS 40.0%
! GCATATTTGCTGAGATGCTT+AGG - chr8_3:36844378-36844397 Msa1287110:intron 40.0%
AATATCGCAGCCAATATCTG+CGG + chr8_3:36844434-36844453 None:intergenic 40.0%
TTGCATATAGAAGACACACC+AGG + chr8_3:36844586-36844605 None:intergenic 40.0%
! CAGCTGAAATCCTGCTATTT+GGG + chr8_3:36844991-36845010 None:intergenic 40.0%
TCAGCTGAAATCCTGCTATT+TGG + chr8_3:36844992-36845011 None:intergenic 40.0%
GTACGCCAACTGAAGAAATC+TGG - chr8_3:36844560-36844579 Msa1287110:intron 45.0%
CAACCCTCCAATTGTTCCAA+AGG - chr8_3:36844621-36844640 Msa1287110:intron 45.0%
! AAATCCTGCTATTTGGGCAC+AGG + chr8_3:36844985-36845004 None:intergenic 45.0%
GTACGCCAACTGAAGAAATC+TGG - chr8_3:36844560-36844579 Msa1287110:intron 45.0%
CAACCCTCCAATTGTTCCAA+AGG - chr8_3:36844621-36844640 Msa1287110:intron 45.0%
! AAATCCTGCTATTTGGGCAC+AGG + chr8_3:36844985-36845004 None:intergenic 45.0%
AGGCTCGAACCTCTGTTCAT+TGG - chr8_3:36844398-36844417 Msa1287110:intron 50.0%
CCAGGCCAGATTTCTTCAGT+TGG + chr8_3:36844568-36844587 None:intergenic 50.0%
CCAACTGAAGAAATCTGGCC+TGG - chr8_3:36844565-36844584 Msa1287110:intron 50.0%
ATTCCCTGAGCTCGAACAGA+TGG - chr8_3:36844744-36844763 Msa1287110:CDS 50.0%
AGACCATCTGTTCGAGCTCA+GGG + chr8_3:36844750-36844769 None:intergenic 50.0%
AAGACCATCTGTTCGAGCTC+AGG + chr8_3:36844751-36844770 None:intergenic 50.0%
TTTGCCTGTGCCCAAATAGC+AGG - chr8_3:36844978-36844997 Msa1287110:CDS 50.0%
AGGCTCGAACCTCTGTTCAT+TGG - chr8_3:36844398-36844417 Msa1287110:intron 50.0%
CCAGGCCAGATTTCTTCAGT+TGG + chr8_3:36844568-36844587 None:intergenic 50.0%
CCAACTGAAGAAATCTGGCC+TGG - chr8_3:36844565-36844584 Msa1287110:intron 50.0%
ATTCCCTGAGCTCGAACAGA+TGG - chr8_3:36844744-36844763 Msa1287110:CDS 50.0%
AGACCATCTGTTCGAGCTCA+GGG + chr8_3:36844750-36844769 None:intergenic 50.0%
AAGACCATCTGTTCGAGCTC+AGG + chr8_3:36844751-36844770 None:intergenic 50.0%
TTTGCCTGTGCCCAAATAGC+AGG - chr8_3:36844978-36844997 Msa1287110:CDS 50.0%
GCTCAGGGAATTCTGCTGCT+AGG + chr8_3:36844735-36844754 None:intergenic 55.0%
GCTCAGGGAATTCTGCTGCT+AGG + chr8_3:36844735-36844754 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 36844293 36845063 36844293 ID=Msa1287110;Name=Msa1287110
chr8_3 mRNA 36844293 36845063 36844293 ID=Msa1287110-mRNA-1;Parent=Msa1287110;Name=Msa1287110-mRNA-1;_AED=0.29;_eAED=0.53;_QI=0|0|0|1|0.66|0.5|4|0|127
chr8_3 exon 36844900 36845063 36844900 ID=Msa1287110-mRNA-1:exon:10667;Parent=Msa1287110-mRNA-1
chr8_3 exon 36844714 36844804 36844714 ID=Msa1287110-mRNA-1:exon:10666;Parent=Msa1287110-mRNA-1
chr8_3 exon 36844591 36844626 36844591 ID=Msa1287110-mRNA-1:exon:10665;Parent=Msa1287110-mRNA-1
chr8_3 exon 36844293 36844385 36844293 ID=Msa1287110-mRNA-1:exon:10664;Parent=Msa1287110-mRNA-1
chr8_3 CDS 36844900 36845063 36844900 ID=Msa1287110-mRNA-1:cds;Parent=Msa1287110-mRNA-1
chr8_3 CDS 36844714 36844804 36844714 ID=Msa1287110-mRNA-1:cds;Parent=Msa1287110-mRNA-1
chr8_3 CDS 36844591 36844626 36844591 ID=Msa1287110-mRNA-1:cds;Parent=Msa1287110-mRNA-1
chr8_3 CDS 36844293 36844385 36844293 ID=Msa1287110-mRNA-1:cds;Parent=Msa1287110-mRNA-1
Gene Sequence

>Msa1287110

ATGGGTAATCCGTGCTATAAAGCACCTGAACTTTTGATGGGTTCTACCAATTACTCAACTGCAGTTGATATCTGGTCTTTGGGATGCATATTTGCTGAGATGCTTAGGCTCGAACCTCTGTTCATTGGTAATGATGAACCGCAGATATTGGCTGCGATATTTTGCTTGCTTGGTACGCCAACTGAAGAAATCTGGCCTGGTGTGTCTTCTATATGCAACTGCATTGAAGCATTCAACCCTCCAATTGTTCCAAAGGGCCTAGCAGCAGAATTCCCTGAGCTCGAACAGATGAATATGCTTTGCCTGTGCCCAAATAGCAGGATTTCAGCTGATGAAGCTCTTGAGCATCCATATCTTCGAATTTTAACCACCCATGATGATTGA
Protein sequence

>Msa1287110

MGNPCYKAPELLMGSTNYSTAVDIWSLGCIFAEMLRLEPLFIGNDEPQILAAIFCLLGTPTEEIWPGVSSICNCIEAFNPPIVPKGLAAEFPELEQMNMLCLCPNSRISADEALEHPYLRILTTHDD*