Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1289440 | ABN08117.1 | 90.164 | 122 | 11 | 1 | 1 | 122 | 1 | 121 | 9.92e-72 | 220 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1289440 | A2Q3B7 | 90.164 | 122 | 11 | 1 | 1 | 122 | 1 | 121 | 4.74e-72 | 220 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1289440 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1289440 | MtrunA17_Chr2g0309121 | 90.164 | 122 | 11 | 1 | 1 | 122 | 1 | 121 | 9.13e-76 | 220 |
| Msa1289440 | MtrunA17_Chr2g0281511 | 84.426 | 122 | 19 | 0 | 1 | 122 | 1 | 122 | 1.33e-72 | 212 |
| Msa1289440 | MtrunA17_Chr3g0081891 | 83.750 | 80 | 13 | 0 | 1 | 80 | 1 | 80 | 8.78e-44 | 137 |
| Msa1289440 | MtrunA17_Chr7g0276661 | 32.061 | 131 | 69 | 4 | 7 | 119 | 38 | 166 | 3.66e-16 | 70.5 |
| Msa1289440 | MtrunA17_Chr0c01g0489371 | 42.857 | 91 | 50 | 1 | 22 | 110 | 224 | 314 | 8.78e-16 | 71.6 |
| Msa1289440 | MtrunA17_Chr8g0356961 | 41.573 | 89 | 50 | 1 | 24 | 110 | 77 | 165 | 4.30e-15 | 67.8 |
| Msa1289440 | MtrunA17_Chr8g0372431 | 27.835 | 97 | 70 | 0 | 24 | 120 | 6 | 102 | 1.17e-13 | 62.0 |
| Msa1289440 | MtrunA17_Chr5g0434641 | 36.264 | 91 | 56 | 1 | 22 | 110 | 208 | 298 | 1.31e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGGTTATTGCTTCTATTT+TGG | 0.132946 | 8_3:+43326870 | Msa1289440:CDS |
| GAAGTCAATGGTACTGAATT+AGG | 0.159497 | 8_3:+43326811 | Msa1289440:CDS |
| TGTATGCTACCGTCGTTAAC+TGG | 0.211996 | 8_3:+43327230 | Msa1289440:CDS |
| AAAATATCGATGTCCATTAT+TGG | 0.234776 | 8_3:+43326842 | Msa1289440:CDS |
| TACCGGTTTCTTCATCAAAA+AGG | 0.272684 | 8_3:-43327063 | None:intergenic |
| TAACCTTATGCTTCCAATAA+TGG | 0.277357 | 8_3:-43326855 | None:intergenic |
| ATCAAAGTCATAAGATTTAC+CGG | 0.326757 | 8_3:-43327080 | None:intergenic |
| CCTCGATGTGTTGCGGAGAA+TGG | 0.338723 | 8_3:+43327013 | Msa1289440:CDS |
| TTTGGATGGAAACAATGTTT+TGG | 0.356448 | 8_3:+43326888 | Msa1289440:CDS |
| TAAGGAAACATGAATGAGAT+TGG | 0.380138 | 8_3:+43326784 | None:intergenic |
| GTACAACTTTGTTCTTGAAA+AGG | 0.417029 | 8_3:+43327153 | Msa1289440:CDS |
| AAGTCAATGGTACTGAATTA+GGG | 0.437113 | 8_3:+43326812 | Msa1289440:CDS |
| TGTCCATTATTGGAAGCATA+AGG | 0.460083 | 8_3:+43326852 | Msa1289440:CDS |
| CGGTCAATAATCCTCCTAAT+CGG | 0.465785 | 8_3:+43327206 | Msa1289440:CDS |
| ACGGTAGCATACATCCGATT+AGG | 0.477261 | 8_3:-43327220 | None:intergenic |
| CTTGAAAAGGACCTAAAAGT+TGG | 0.486000 | 8_3:+43327166 | Msa1289440:CDS |
| AGAAATACATAGGGAGAGAA+TGG | 0.518052 | 8_3:+43327131 | Msa1289440:CDS |
| GTATGCTACCGTCGTTAACT+GGG | 0.522932 | 8_3:+43327231 | Msa1289440:CDS |
| AACATGCATCAGAAATACAT+AGG | 0.535430 | 8_3:+43327121 | Msa1289440:CDS |
| GAGATTGGAGTTGAAGTCAA+TGG | 0.575541 | 8_3:+43326799 | Msa1289440:CDS |
| ACATGCATCAGAAATACATA+GGG | 0.587826 | 8_3:+43327122 | Msa1289440:CDS |
| GTAGCATACATCCGATTAGG+AGG | 0.600441 | 8_3:-43327217 | None:intergenic |
| CCATTCTCCGCAACACATCG+AGG | 0.699602 | 8_3:-43327013 | None:intergenic |
| GAATCAGTCCCAGTTAACGA+CGG | 0.733495 | 8_3:-43327239 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATATCGATGTCCATTAT+TGG | + | chr8_3:43326842-43326861 | Msa1289440:CDS | 25.0% |
| !! | TAAGGTTATTGCTTCTATTT+TGG | + | chr8_3:43326870-43326889 | Msa1289440:CDS | 25.0% |
| ! | ATCAAAGTCATAAGATTTAC+CGG | - | chr8_3:43327083-43327102 | None:intergenic | 25.0% |
| ! | AAGTCAATGGTACTGAATTA+GGG | + | chr8_3:43326812-43326831 | Msa1289440:CDS | 30.0% |
| TAACCTTATGCTTCCAATAA+TGG | - | chr8_3:43326858-43326877 | None:intergenic | 30.0% | |
| !! | GTTATTGCTTCTATTTTGGA+TGG | + | chr8_3:43326874-43326893 | Msa1289440:CDS | 30.0% |
| ! | TTTGGATGGAAACAATGTTT+TGG | + | chr8_3:43326888-43326907 | Msa1289440:CDS | 30.0% |
| AACATGCATCAGAAATACAT+AGG | + | chr8_3:43327121-43327140 | Msa1289440:CDS | 30.0% | |
| ACATGCATCAGAAATACATA+GGG | + | chr8_3:43327122-43327141 | Msa1289440:CDS | 30.0% | |
| GTACAACTTTGTTCTTGAAA+AGG | + | chr8_3:43327153-43327172 | Msa1289440:CDS | 30.0% | |
| ! | AAATAACGTCACCAACTTTT+AGG | - | chr8_3:43327180-43327199 | None:intergenic | 30.0% |
| ! | GAAGTCAATGGTACTGAATT+AGG | + | chr8_3:43326811-43326830 | Msa1289440:CDS | 35.0% |
| ! | TGTCCATTATTGGAAGCATA+AGG | + | chr8_3:43326852-43326871 | Msa1289440:CDS | 35.0% |
| !!! | GGAAACAATGTTTTGGTTAG+TGG | + | chr8_3:43326895-43326914 | Msa1289440:intron | 35.0% |
| ! | CTCCTTTTTGATGAAGAAAC+CGG | + | chr8_3:43327061-43327080 | Msa1289440:CDS | 35.0% |
| !! | TACCGGTTTCTTCATCAAAA+AGG | - | chr8_3:43327066-43327085 | None:intergenic | 35.0% |
| AGAAATACATAGGGAGAGAA+TGG | + | chr8_3:43327131-43327150 | Msa1289440:CDS | 35.0% | |
| CTTGAAAAGGACCTAAAAGT+TGG | + | chr8_3:43327166-43327185 | Msa1289440:CDS | 35.0% | |
| !! | TGGTGACGTTATTTTGTTCA+CGG | + | chr8_3:43327186-43327205 | Msa1289440:CDS | 35.0% |
| GAGATTGGAGTTGAAGTCAA+TGG | + | chr8_3:43326799-43326818 | Msa1289440:CDS | 40.0% | |
| CGGTCAATAATCCTCCTAAT+CGG | + | chr8_3:43327206-43327225 | Msa1289440:CDS | 40.0% | |
| ! | GCATTTTCCTCGATGTGTTG+CGG | + | chr8_3:43327006-43327025 | Msa1289440:intron | 45.0% |
| GTAGCATACATCCGATTAGG+AGG | - | chr8_3:43327220-43327239 | None:intergenic | 45.0% | |
| ACGGTAGCATACATCCGATT+AGG | - | chr8_3:43327223-43327242 | None:intergenic | 45.0% | |
| TGTATGCTACCGTCGTTAAC+TGG | + | chr8_3:43327230-43327249 | Msa1289440:CDS | 45.0% | |
| GTATGCTACCGTCGTTAACT+GGG | + | chr8_3:43327231-43327250 | Msa1289440:CDS | 45.0% | |
| CCATTCTCCGCAACACATCG+AGG | - | chr8_3:43327016-43327035 | None:intergenic | 55.0% | |
| CCTCGATGTGTTGCGGAGAA+TGG | + | chr8_3:43327013-43327032 | Msa1289440:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 43326793 | 43327258 | 43326793 | ID=Msa1289440;Name=Msa1289440 |
| chr8_3 | mRNA | 43326793 | 43327258 | 43326793 | ID=Msa1289440-mRNA-1;Parent=Msa1289440;Name=Msa1289440-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|122 |
| chr8_3 | exon | 43326793 | 43326909 | 43326793 | ID=Msa1289440-mRNA-1:exon:11883;Parent=Msa1289440-mRNA-1 |
| chr8_3 | exon | 43327007 | 43327258 | 43327007 | ID=Msa1289440-mRNA-1:exon:11884;Parent=Msa1289440-mRNA-1 |
| chr8_3 | CDS | 43326793 | 43326909 | 43326793 | ID=Msa1289440-mRNA-1:cds;Parent=Msa1289440-mRNA-1 |
| chr8_3 | CDS | 43327007 | 43327258 | 43327007 | ID=Msa1289440-mRNA-1:cds;Parent=Msa1289440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
