AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1289700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1289700 MtrunA17_Chr8g0363921 96.140 285 4 2 1 285 1 278 0.0 551
Msa1289700 MtrunA17_Chr5g0415291 70.046 217 45 6 75 285 37 239 1.05e-95 281
Msa1289700 MtrunA17_Chr7g0234761 53.659 205 73 6 84 276 234 428 1.70e-52 179
Msa1289700 MtrunA17_Chr8g0339091 53.297 182 73 4 84 256 212 390 4.52e-51 174
Msa1289700 MtrunA17_Chr5g0421111 71.930 114 30 1 134 247 343 454 1.85e-47 166
Msa1289700 MtrunA17_Chr8g0365941 54.118 170 68 4 79 247 234 394 5.10e-47 163
Msa1289700 MtrunA17_Chr1g0152131 38.591 298 126 10 5 257 74 359 5.69e-46 159
Msa1289700 MtrunA17_Chr4g0035101 55.484 155 53 2 110 251 85 236 3.76e-45 155
Msa1289700 MtrunA17_Chr3g0144641 60.417 144 44 4 123 254 150 292 6.71e-45 156
Msa1289700 MtrunA17_Chr8g0385911 88.000 75 9 0 155 229 142 216 3.11e-40 142
Msa1289700 MtrunA17_Chr3g0131771 75.824 91 22 0 139 229 128 218 9.18e-40 141
Msa1289700 MtrunA17_Chr7g0258161 68.478 92 29 0 152 243 167 258 1.54e-37 135
Msa1289700 MtrunA17_Chr1g0174161 69.412 85 26 0 155 239 157 241 4.20e-36 131
Msa1289700 MtrunA17_Chr6g0484271 50.000 140 53 3 99 238 113 235 1.01e-33 124
Msa1289700 MtrunA17_Chr4g0074161 59.756 82 32 1 142 223 190 270 3.98e-22 95.1
Msa1289700 MtrunA17_Chr1g0190731 58.974 78 26 2 146 223 186 257 6.24e-20 88.2
Msa1289700 MtrunA17_Chr1g0182791 47.561 82 41 2 160 241 319 398 2.76e-15 75.9
Msa1289700 MtrunA17_Chr2g0329041 38.194 144 72 5 88 220 257 394 3.69e-15 75.1
Msa1289700 MtrunA17_Chr1g0193321 50.000 64 31 1 160 223 464 526 4.04e-15 75.5
Msa1289700 MtrunA17_Chr4g0032951 55.385 65 29 0 158 222 355 419 1.59e-14 73.2
Msa1289700 MtrunA17_Chr1g0174271 48.101 79 36 1 146 224 291 364 1.32e-13 70.5
Msa1289700 MtrunA17_Chr4g0014771 49.231 65 33 0 158 222 118 182 3.30e-13 67.0
Msa1289700 MtrunA17_Chr7g0258181 44.595 74 41 0 158 231 243 316 4.57e-13 68.2
Msa1289700 MtrunA17_Chr1g0184841 60.606 66 25 1 158 223 120 184 4.91e-13 67.8
Msa1289700 MtrunA17_Chr1g0152651 49.231 65 32 1 158 222 274 337 1.88e-12 66.6
Msa1289700 MtrunA17_Chr1g0153241 44.615 65 35 1 160 224 87 150 3.49e-12 65.1
Msa1289700 MtrunA17_Chr3g0134741 35.659 129 69 3 107 222 165 292 4.35e-12 65.5
Msa1289700 MtrunA17_Chr8g0351881 47.692 65 34 0 158 222 316 380 7.78e-12 65.1
Msa1289700 MtrunA17_Chr5g0393721 50.000 64 31 1 159 222 225 287 1.02e-11 64.3
Msa1289700 MtrunA17_Chr7g0262691 46.875 64 33 1 160 223 254 316 1.20e-11 64.7
Msa1289700 MtrunA17_Chr7g0271251 48.438 64 32 1 160 223 434 496 1.77e-11 64.3
Msa1289700 MtrunA17_Chr3g0095391 46.875 64 33 1 160 223 311 373 2.67e-11 63.5
Msa1289700 MtrunA17_Chr5g0402031 50.000 56 27 1 169 224 116 170 9.66e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1289700 AT1G59640.1 58.020 293 86 11 1 285 1 264 5.44e-96 283
Msa1289700 AT1G59640.2 56.800 250 72 10 1 243 1 221 7.75e-75 232
Msa1289700 AT5G62610.2 51.525 295 119 12 1 285 1 281 8.10e-71 220
Msa1289700 AT5G62610.1 51.525 295 119 12 1 285 1 281 8.10e-71 220
Msa1289700 AT3G07340.2 58.125 160 45 3 84 243 206 343 5.12e-49 168
Msa1289700 AT3G07340.1 58.125 160 45 3 84 243 206 343 8.91e-49 168
Msa1289700 AT1G26260.3 55.696 158 64 2 92 248 110 262 5.94e-46 158
Msa1289700 AT5G48560.2 77.451 102 19 3 143 243 289 387 6.52e-46 160
Msa1289700 AT5G48560.1 77.451 102 19 3 143 243 289 387 1.10e-45 160
Msa1289700 AT1G26260.5 55.696 158 64 2 92 248 139 291 1.19e-45 158
Msa1289700 AT1G26260.4 55.696 158 64 2 92 248 161 313 2.12e-45 157
Msa1289700 AT1G26260.1 55.696 158 64 2 92 248 161 313 2.12e-45 157
Msa1289700 AT1G26260.6 55.696 158 64 2 92 248 161 313 2.12e-45 157
Msa1289700 AT1G26260.2 55.696 158 64 2 92 248 161 313 2.12e-45 157
Msa1289700 AT1G68920.3 75.758 99 24 0 148 246 292 390 5.98e-45 159
Msa1289700 AT1G68920.1 75.758 99 24 0 148 246 293 391 6.27e-45 158
Msa1289700 AT1G68920.4 75.758 99 24 0 148 246 293 391 6.27e-45 158
Msa1289700 AT1G68920.2 75.758 99 24 0 148 246 293 391 6.27e-45 158
Msa1289700 AT3G23690.1 61.029 136 42 3 97 232 141 265 9.54e-44 153
Msa1289700 AT5G50915.3 56.115 139 49 3 93 229 79 207 8.69e-43 147
Msa1289700 AT5G50915.4 56.115 139 49 3 93 229 79 207 8.69e-43 147
Msa1289700 AT5G50915.1 56.522 138 48 3 93 228 79 206 1.74e-42 147
Msa1289700 AT5G50915.2 56.522 138 48 3 93 228 79 206 1.74e-42 147
Msa1289700 AT1G10120.2 69.388 98 30 0 149 246 197 294 2.48e-42 149
Msa1289700 AT1G10120.1 69.388 98 30 0 149 246 197 294 2.48e-42 149
Msa1289700 AT4G36540.1 52.740 146 67 1 101 244 82 227 7.79e-42 146
Msa1289700 AT4G36540.2 53.472 144 65 1 101 242 82 225 2.01e-41 145
Msa1289700 AT2G18300.2 70.103 97 29 0 148 244 175 271 6.42e-41 144
Msa1289700 AT4G34530.1 82.500 80 14 0 152 231 166 245 1.40e-40 144
Msa1289700 AT2G18300.3 70.103 97 29 0 148 244 175 271 1.43e-40 144
Msa1289700 AT2G18300.1 71.579 95 27 0 148 242 175 269 2.01e-40 143
Msa1289700 AT2G42300.4 69.318 88 27 0 152 239 179 266 2.96e-38 136
Msa1289700 AT3G57800.2 68.085 94 30 0 152 245 198 291 8.40e-38 137
Msa1289700 AT2G42300.1 65.957 94 32 0 152 245 179 272 1.17e-37 135
Msa1289700 AT1G25330.1 48.507 134 60 2 115 246 66 192 3.84e-34 124
Msa1289700 AT1G18400.2 74.667 75 19 0 156 230 143 217 8.08e-34 124
Msa1289700 AT1G18400.1 74.667 75 19 0 156 230 143 217 1.33e-33 123
Msa1289700 AT1G73830.2 69.512 82 25 0 157 238 146 227 2.61e-33 122
Msa1289700 AT1G73830.1 69.512 82 25 0 157 238 146 227 2.71e-33 122
Msa1289700 AT3G57800.1 45.390 141 30 1 152 245 198 338 6.00e-29 114
Msa1289700 AT2G24260.2 63.014 73 26 1 151 223 28 99 1.78e-22 94.0
Msa1289700 AT2G24260.1 63.014 73 26 1 151 223 131 202 1.33e-21 93.2
Msa1289700 AT4G30980.1 68.182 66 20 1 158 223 130 194 5.50e-21 90.9
Msa1289700 AT4G30980.2 68.182 66 20 1 158 223 178 242 7.82e-21 91.3
Msa1289700 AT5G58010.1 61.842 76 28 1 148 223 89 163 8.37e-21 90.1
Msa1289700 AT2G42300.5 78.723 47 10 0 152 198 179 225 2.74e-19 84.7
Msa1289700 AT2G42300.3 78.723 47 10 0 152 198 179 225 2.74e-19 84.7
Msa1289700 AT2G42300.2 80.435 46 9 0 152 197 179 224 3.74e-19 84.7
Msa1289700 AT4G02590.3 49.515 103 46 3 126 223 46 147 1.43e-15 75.1
Msa1289700 AT4G02590.2 49.515 103 46 3 126 223 109 210 2.37e-15 75.1
Msa1289700 AT4G02590.1 49.515 103 46 3 126 223 109 210 2.37e-15 75.1
Msa1289700 AT2G20180.7 51.562 64 30 1 160 223 209 271 5.63e-15 74.7
Msa1289700 AT2G20180.1 51.562 64 30 1 160 223 209 271 5.63e-15 74.7
Msa1289700 AT2G20180.5 51.562 64 30 1 160 223 209 271 5.63e-15 74.7
Msa1289700 AT2G20180.4 51.562 64 30 1 160 223 280 342 6.77e-15 74.7
Msa1289700 AT2G20180.6 51.562 64 30 1 160 223 280 342 6.77e-15 74.7
Msa1289700 AT2G20180.2 51.562 64 30 1 160 223 280 342 6.77e-15 74.7
Msa1289700 AT2G20180.3 51.562 64 30 1 160 223 280 342 6.77e-15 74.7
Msa1289700 AT1G09530.3 50.000 64 31 1 160 223 339 401 8.31e-15 74.7
Msa1289700 AT1G09530.2 50.000 64 31 1 160 223 339 401 8.31e-15 74.7
Msa1289700 AT1G09530.5 50.000 64 31 1 160 223 339 401 8.31e-15 74.7
Msa1289700 AT1G09530.1 50.000 64 31 1 160 223 339 401 8.31e-15 74.7
Msa1289700 AT1G09530.4 50.000 64 31 1 160 223 339 401 8.31e-15 74.7
Msa1289700 AT1G09530.6 50.000 64 31 1 160 223 339 401 8.31e-15 74.7
Msa1289700 AT1G03040.1 48.544 103 47 3 126 223 107 208 2.23e-14 72.4
Msa1289700 AT1G03040.3 48.544 103 47 3 126 223 107 208 2.40e-14 72.4
Msa1289700 AT4G28800.1 44.000 75 41 1 161 235 252 325 1.60e-13 70.9
Msa1289700 AT1G03040.2 54.217 83 35 2 143 223 122 203 2.33e-13 69.3
Msa1289700 AT2G20180.8 50.820 61 29 1 160 220 280 339 3.33e-13 69.3
Msa1289700 AT2G42280.1 50.000 64 32 0 158 221 279 342 4.10e-13 69.3
Msa1289700 AT1G51140.1 53.125 64 30 0 158 221 304 367 4.28e-13 69.3
Msa1289700 AT4G28815.2 51.724 58 27 1 161 218 71 127 4.59e-13 67.8
Msa1289700 AT4G09180.1 46.269 67 36 0 156 222 182 248 7.47e-13 67.4
Msa1289700 AT2G42280.3 51.515 66 30 1 158 221 279 344 8.56e-13 68.2
Msa1289700 AT4G28800.2 45.833 72 37 2 161 231 264 334 1.14e-12 68.2
Msa1289700 AT4G28790.1 35.200 125 61 5 160 280 273 381 1.44e-12 67.8
Msa1289700 AT4G28815.1 51.724 58 27 1 161 218 147 203 1.53e-12 67.0
Msa1289700 AT2G46970.1 43.836 73 35 2 156 223 216 287 2.16e-12 67.4
Msa1289700 AT2G46970.2 43.836 73 35 2 156 223 185 256 2.46e-12 67.0
Msa1289700 AT2G46970.3 43.836 73 35 2 156 223 194 265 2.57e-12 67.0
Msa1289700 AT4G33880.1 42.857 84 45 2 141 222 247 329 3.85e-12 66.2
Msa1289700 AT1G35460.1 46.154 65 35 0 158 222 181 245 5.44e-12 65.1
Msa1289700 AT4G00050.2 47.692 65 33 1 158 222 105 168 1.31e-11 64.3
Msa1289700 AT2G14760.1 49.231 65 32 1 158 222 237 300 2.12e-11 63.9
Msa1289700 AT1G27740.1 49.231 65 32 1 158 222 167 230 2.21e-11 63.2
Msa1289700 AT4G00050.1 47.692 65 33 1 158 222 207 270 2.24e-11 64.3
Msa1289700 AT4G00050.3 47.692 65 33 1 158 222 207 270 2.59e-11 63.9
Msa1289700 AT4G36930.1 47.541 61 31 1 164 224 197 256 8.27e-11 62.4

Find 117 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTTCAATTTAGGTTAT+TGG 0.179282 8_3:-43990585 Msa1289700:intron
CCATACCTTTGTGTAGAAAA+TGG 0.194010 8_3:+43989508 None:intergenic
GTGTACTATAGTGGACTTTC+AGG 0.196499 8_3:-43989412 Msa1289700:three_prime_UTR
TGTTTAACACTTACATCTTT+TGG 0.232272 8_3:+43990347 None:intergenic
TTTCTTCAATTTAGGTTATT+GGG 0.256415 8_3:-43990584 Msa1289700:intron
CAGTTTCCAGCGGCGATTAA+TGG 0.284846 8_3:-43992340 Msa1289700:CDS
TTTAGTTCCTGGTTGTAATA+AGG 0.288451 8_3:-43990821 Msa1289700:intron
CCGCAGTTTGGGAACGGTTT+AGG 0.291510 8_3:-43992289 Msa1289700:CDS
AGTTGAAAACGGTTCGGTTC+GGG 0.306795 8_3:-43992467 Msa1289700:five_prime_UTR
GACTAAGAAGGCCGCAGTTT+GGG 0.309614 8_3:-43992300 Msa1289700:CDS
GGCATTGGCACCGCCATTAC+TGG 0.323699 8_3:+43992129 None:intergenic
CAACAGGCTGCTTCAGTTTG+TGG 0.325216 8_3:-43989470 Msa1289700:three_prime_UTR
CTCAATATTGTGTACTATAG+TGG 0.335669 8_3:-43989421 Msa1289700:three_prime_UTR
ATTCTACAGGATTTAGTTCC+TGG 0.338943 8_3:-43990832 Msa1289700:CDS
TTAGGACAGTTTGGTGAGTT+TGG 0.345423 8_3:-43992271 Msa1289700:CDS
AATGGCGGCGGAGGTGGATT+AGG 0.359141 8_3:-43992322 Msa1289700:CDS
GAGTTGAAAACGGTTCGGTT+CGG 0.365096 8_3:-43992468 Msa1289700:five_prime_UTR
CATCGCTCCTGGATCCATTT+CGG 0.370295 8_3:+43992429 None:intergenic
CGTTTAGTTTAGCGGAGATC+TGG 0.375505 8_3:-43992363 Msa1289700:CDS
CAACAAACGTTTGATCTTGC+CGG 0.377074 8_3:-43989769 Msa1289700:CDS
ATTACCATCCCGGTTTAAAC+CGG 0.385440 8_3:+43992246 None:intergenic
ACCTGCATATGTAACCATTC+TGG 0.392490 8_3:+43989697 None:intergenic
TGGACAATAAACAGGCTAGA+AGG 0.396885 8_3:-43990879 Msa1289700:intron
CCCTCGTTCATCATCGCTCC+TGG 0.400423 8_3:+43992418 None:intergenic
ACATCCATGTTCGAGCTAGA+AGG 0.403285 8_3:-43991239 Msa1289700:CDS
CAGATCTCCGCTAAACTAAA+CGG 0.416576 8_3:+43992364 None:intergenic
GTTGAAGGAGAAGTCAGTTC+AGG 0.418689 8_3:-43991318 Msa1289700:CDS
GGAGCATTTGAAGGATGAAA+TGG 0.421937 8_3:+43989594 None:intergenic
CATACCTTTGTGTAGAAAAT+GGG 0.425858 8_3:+43989509 None:intergenic
GCGATTAATGGCGGCGGAGG+TGG 0.426598 8_3:-43992328 Msa1289700:CDS
AGTTTGGACCCGGTTTAAAC+CGG 0.426792 8_3:-43992255 Msa1289700:CDS
GGACTAAGAAGGCCGCAGTT+TGG 0.428490 8_3:-43992301 Msa1289700:CDS
TTGAAGTTAGTGAACCGAAA+TGG 0.445482 8_3:-43992443 Msa1289700:exon
CAGTTTGGTGAGTTTGGACC+CGG 0.446797 8_3:-43992265 Msa1289700:CDS
GGAAAACGGACTAAAGCATC+AGG 0.450766 8_3:-43991369 Msa1289700:CDS
CTTGTAGCTTGTGAAACAAA+TGG 0.451137 8_3:+43989742 None:intergenic
GGTTTAAACCGGGATGGTAA+TGG 0.452515 8_3:-43992244 Msa1289700:CDS
TACGACGCCGTTTAGTTTAG+CGG 0.455033 8_3:-43992371 Msa1289700:CDS
TGAAAGGATGAAAATTCTAC+AGG 0.461627 8_3:-43990845 Msa1289700:CDS
TAGTGAACCGAAATGGATCC+AGG 0.464407 8_3:-43992436 Msa1289700:exon
GGTTCGGTGCGTGATCTCCT+TGG 0.468651 8_3:+43992628 None:intergenic
GTCGTATTTCCATTCCCATT+CGG 0.470342 8_3:+43992388 None:intergenic
CATCCATGTTCGAGCTAGAA+GGG 0.474849 8_3:-43991238 Msa1289700:CDS
GATTAATGTTGAGTTGAAAA+CGG 0.477674 8_3:-43992478 Msa1289700:five_prime_UTR
GGTTCATTTCCGAATGGGAA+TGG 0.479304 8_3:-43992397 Msa1289700:CDS
GGGAACGGTTTAGGACAGTT+TGG 0.481836 8_3:-43992280 Msa1289700:CDS
ACCAGAATGGTTACATATGC+AGG 0.483200 8_3:-43989698 Msa1289700:CDS
TATAATATAGGAGCATTTGA+AGG 0.491142 8_3:+43989585 None:intergenic
AACCATGGTGGTGTTGGTGG+TGG 0.493213 8_3:-43992199 Msa1289700:CDS
ACATGGATGTAATCTTGCTT+AGG 0.502301 8_3:+43991252 None:intergenic
ACGAGGGTTCATTTCCGAAT+GGG 0.502721 8_3:-43992402 Msa1289700:CDS
AACAAGAATGAGGGTGGTGA+TGG 0.502949 8_3:-43991345 Msa1289700:CDS
CCGCCGCCATTAATCGCCGC+TGG 0.503828 8_3:+43992334 None:intergenic
TTGAACCATGGTGGTGTTGG+TGG 0.507286 8_3:-43992202 Msa1289700:CDS
GCGGCGATTAATGGCGGCGG+AGG 0.511591 8_3:-43992331 Msa1289700:CDS
TGGAGGAAAGGCTTCGATGC+CGG 0.515251 8_3:+43990367 None:intergenic
GCAGAGGTTCTTCACCAGAA+TGG 0.515410 8_3:-43989711 Msa1289700:CDS
TCGTGTTTGTTTGTGTAGCA+AGG 0.516464 8_3:-43989272 Msa1289700:three_prime_UTR
TGGATGTAATCTTGCTTAGG+AGG 0.520256 8_3:+43991255 None:intergenic
GGACAATAAACAGGCTAGAA+GGG 0.523957 8_3:-43990878 Msa1289700:intron
ATGTTGAGTTGAAAACGGTT+CGG 0.525360 8_3:-43992473 Msa1289700:five_prime_UTR
GAGAGAGAGAGTGTTAGGTT+CGG 0.527541 8_3:+43992612 None:intergenic
ACTCCGGTCCATTACCATCC+CGG 0.528802 8_3:+43992236 None:intergenic
TTAGGTTATTGGGAAAGCAT+TGG 0.529281 8_3:-43990574 Msa1289700:intron
GTTTGGACCCGGTTTAAACC+GGG 0.537559 8_3:-43992254 Msa1289700:CDS
TCTGGTGAAGAACCTCTGCT+TGG 0.539168 8_3:+43989715 None:intergenic
AAGTGTGTTTCCACCAGTAA+TGG 0.540169 8_3:-43992142 Msa1289700:CDS
TTACCATCCCGGTTTAAACC+GGG 0.553757 8_3:+43992247 None:intergenic
GCACCATTCTCCACTGTCCA+AGG 0.555247 8_3:-43992645 Msa1289700:five_prime_UTR
CCAGCGGCGATTAATGGCGG+CGG 0.557594 8_3:-43992334 Msa1289700:CDS
TTACATATGCAGGTAGGTGG+TGG 0.558363 8_3:-43989688 Msa1289700:CDS
AGAAGGCCGCAGTTTGGGAA+CGG 0.559428 8_3:-43992295 Msa1289700:CDS
CTTAAGGTGTTAAAGTCCAA+TGG 0.560546 8_3:-43989546 Msa1289700:three_prime_UTR
AGCATCAGGGAACAAGAATG+AGG 0.561192 8_3:-43991355 Msa1289700:CDS
AGAATGATAGTGATGGAAAA+CGG 0.563521 8_3:-43991383 Msa1289700:CDS
AACGAGGGTTCATTTCCGAA+TGG 0.564078 8_3:-43992403 Msa1289700:CDS
TTGTGAAACAAATGGCATGC+CGG 0.566973 8_3:+43989750 None:intergenic
TGCGTGATCTCCTTGGACAG+TGG 0.568345 8_3:+43992635 None:intergenic
GACCCCTTCTAGCTCGAACA+TGG 0.574237 8_3:+43991235 None:intergenic
AAACCGGGATGGTAATGGAC+CGG 0.576710 8_3:-43992239 Msa1289700:CDS
GAAGGGAAAAGATTAGTGAA+AGG 0.577890 8_3:-43990861 Msa1289700:CDS
GGTGGTGATGGTAAAGTTGA+AGG 0.578873 8_3:-43991333 Msa1289700:CDS
GGAATGAAGAAAGTGTCCAT+TGG 0.578937 8_3:+43989530 None:intergenic
TTAGGAGGGTCAGAAGGAGG+TGG 0.581023 8_3:+43991270 None:intergenic
TCTCCTTGGACAGTGGAGAA+TGG 0.582146 8_3:+43992642 None:intergenic
TTTCCAGCGGCGATTAATGG+CGG 0.585609 8_3:-43992337 Msa1289700:CDS
GTTAATTCAAGACTCAATAC+CGG 0.586424 8_3:-43990386 Msa1289700:CDS
GTGTTGGTGGTGGTAAGAAG+AGG 0.596088 8_3:-43992189 Msa1289700:CDS
ATCACTTCAGCGCCAAGTGG+AGG 0.597164 8_3:-43990523 Msa1289700:intron
TCTTGCTTAGGAGGGTCAGA+AGG 0.603501 8_3:+43991264 None:intergenic
TTCAAAACAACTCTTGACTC+CGG 0.616543 8_3:+43992220 None:intergenic
AACATTACCTTATTACAACC+AGG 0.621430 8_3:+43990814 None:intergenic
GCATCAGGGAACAAGAATGA+GGG 0.629320 8_3:-43991354 Msa1289700:CDS
GGATGTAATCTTGCTTAGGA+GGG 0.631066 8_3:+43991256 None:intergenic
TCCAGGAGCGATGATGAACG+AGG 0.631852 8_3:-43992419 Msa1289700:CDS
GAGGTGGATTAGGACTAAGA+AGG 0.632789 8_3:-43992312 Msa1289700:CDS
TAATGGCGGTGCCAATGCCG+TGG 0.636011 8_3:-43992125 Msa1289700:intron
TGCTTAGGAGGGTCAGAAGG+AGG 0.637170 8_3:+43991267 None:intergenic
ATCCATGTTCGAGCTAGAAG+GGG 0.638122 8_3:-43991237 Msa1289700:CDS
GGACCCGGTTTAAACCGGGA+TGG 0.638557 8_3:-43992250 Msa1289700:CDS
GCTACAAGAGAGCCAAGCAG+AGG 0.640109 8_3:-43989727 Msa1289700:CDS
TTACTGGTGGAAACACACTT+AGG 0.651146 8_3:+43992145 None:intergenic
ATTGGCACCGCCATTACTGG+TGG 0.652469 8_3:+43992132 None:intergenic
TGGTTACATATGCAGGTAGG+TGG 0.652770 8_3:-43989691 Msa1289700:CDS
GGAGATCTGGCAGTTTCCAG+CGG 0.658016 8_3:-43992350 Msa1289700:CDS
TCAATCACTTCAGCGCCAAG+TGG 0.659625 8_3:-43990526 Msa1289700:CDS
TTAGAATAGATACCTCCACT+TGG 0.671733 8_3:+43990511 None:intergenic
TACCACCACCAACACCACCA+TGG 0.672358 8_3:+43992197 None:intergenic
TGTGTTTCCACCAGTAATGG+CGG 0.675613 8_3:-43992139 Msa1289700:CDS
TCAGGGAACAAGAATGAGGG+TGG 0.675624 8_3:-43991351 Msa1289700:CDS
AGTCAGTTCAGGAAAGCATG+CGG 0.677155 8_3:-43991307 Msa1289700:CDS
GAATGGTTACATATGCAGGT+AGG 0.689550 8_3:-43989694 Msa1289700:CDS
CCAGGAGCGATGATGAACGA+GGG 0.693487 8_3:-43992418 Msa1289700:CDS
TTACGCGTTCAGCAAGACTG+TGG 0.703714 8_3:+43991199 None:intergenic
GAAAACGGACTAAAGCATCA+GGG 0.713041 8_3:-43991368 Msa1289700:CDS
CCTAAACCGTTCCCAAACTG+CGG 0.713413 8_3:+43992289 None:intergenic
ATTGTGCAGAATGATAGTGA+TGG 0.726537 8_3:-43991390 Msa1289700:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTTTTTTAGTTATATT+TGG - chr8_3:43989859-43989878 Msa1289700:intron 10.0%
!!! TATTAAATATATGTATTTTG+GGG - chr8_3:43989930-43989949 Msa1289700:intron 10.0%
!! TTTAATGAATATACTTAAAA+CGG - chr8_3:43989991-43990010 Msa1289700:intron 10.0%
!!! TGTGTTTTTTATTTTATTAT+TGG - chr8_3:43990068-43990087 Msa1289700:intron 10.0%
!!! TTCATTTTTGATTTTAAAAT+TGG - chr8_3:43990239-43990258 Msa1289700:intron 10.0%
!! TAACTAAATTATTACAATAA+AGG + chr8_3:43990461-43990480 None:intergenic 10.0%
!! TTAATGGAATTATTATTATT+TGG - chr8_3:43990523-43990542 Msa1289700:intron 10.0%
!!! TCATTTTTGTTTTATAATAT+AGG + chr8_3:43992366-43992385 None:intergenic 10.0%
!!! TTTTTTTAGTTATATTTGGA+AGG - chr8_3:43989863-43989882 Msa1289700:intron 15.0%
!!! GTTTTTAATCATTGTATTTT+TGG - chr8_3:43990024-43990043 Msa1289700:intron 15.0%
!!! AACATAAAAGTTTTTAAAAG+AGG + chr8_3:43990778-43990797 None:intergenic 15.0%
!!! ACTTTTATGTTATTGTTTTT+AGG - chr8_3:43990787-43990806 Msa1289700:intron 15.0%
!!! TTAAGGAAAATGTAAATTTT+TGG - chr8_3:43990986-43991005 Msa1289700:intron 15.0%
!! ATAATAATAAACCAAATGAA+AGG - chr8_3:43991209-43991228 Msa1289700:CDS 15.0%
!! AGTTTCAATATAATAATCAA+GGG + chr8_3:43991616-43991635 None:intergenic 15.0%
!! AAACAAAAATGATATACTTA+AGG - chr8_3:43992374-43992393 Msa1289700:CDS 15.0%
!! AGAAAAAAAAATGAATACCA+CGG + chr8_3:43989831-43989850 None:intergenic 20.0%
!!! AAGATTTTGCTTCATTTTTT+TGG - chr8_3:43990115-43990134 Msa1289700:intron 20.0%
!!! AGATTTTGCTTCATTTTTTT+GGG - chr8_3:43990116-43990135 Msa1289700:intron 20.0%
!!! CATTTTTTTGGGTATTTAAA+AGG - chr8_3:43990127-43990146 Msa1289700:intron 20.0%
!!! AATTTTTCTCCAGAATAATT+AGG + chr8_3:43990438-43990457 None:intergenic 20.0%
!! ATATGTGTATTTGATGTATT+TGG - chr8_3:43990484-43990503 Msa1289700:intron 20.0%
!!! ATGATGTTAAATCTTGTTTA+AGG - chr8_3:43990969-43990988 Msa1289700:intron 20.0%
!! TTTAGTAATTTCCTTTCATT+TGG + chr8_3:43991223-43991242 None:intergenic 20.0%
!!! AACTGTTATTTTCTTTTCAT+GGG + chr8_3:43991269-43991288 None:intergenic 20.0%
!!! TAACTGTTATTTTCTTTTCA+TGG + chr8_3:43991270-43991289 None:intergenic 20.0%
!!! TTTCTTCAATTTAGGTTATT+GGG - chr8_3:43991352-43991371 Msa1289700:CDS 20.0%
!!! ATCTATTCTAATTCTGATTT+TGG - chr8_3:43991437-43991456 Msa1289700:intron 20.0%
!! GAGTTTCAATATAATAATCA+AGG + chr8_3:43991617-43991636 None:intergenic 20.0%
!!! GGAAAATTTTCAAATAAAGA+GGG + chr8_3:43991665-43991684 None:intergenic 20.0%
!!! AGGAAAATTTTCAAATAAAG+AGG + chr8_3:43991666-43991685 None:intergenic 20.0%
!! CATCATTGTTTAAAAAACAT+TGG - chr8_3:43992104-43992123 Msa1289700:intron 20.0%
!!! CTCTTTTTTTTTTTTTTGGT+TGG - chr8_3:43992547-43992566 Msa1289700:five_prime_UTR 20.0%
!!! GATTAATGTTGAGTTGAAAA+CGG - chr8_3:43989458-43989477 Msa1289700:three_prime_UTR 25.0%
!! TATGCAAACTTATTCAAGTT+TGG - chr8_3:43990262-43990281 Msa1289700:intron 25.0%
! TCATAAAACGGTAGATAATT+GGG + chr8_3:43990363-43990382 None:intergenic 25.0%
! TTGCAATCATTGTCATAAAA+CGG + chr8_3:43990375-43990394 None:intergenic 25.0%
!! TTGATGTATTTGGAATTAGT+CGG - chr8_3:43990494-43990513 Msa1289700:intron 25.0%
!!! TTTTAAAAGAGGTGAGTAAA+GGG + chr8_3:43990767-43990786 None:intergenic 25.0%
!!! TTTTTAAAAGAGGTGAGTAA+AGG + chr8_3:43990768-43990787 None:intergenic 25.0%
!!! GTAAATTTTTGGTTTTGTTG+CGG - chr8_3:43990997-43991016 Msa1289700:intron 25.0%
! AACATTCCAATTAAAAACAG+AGG + chr8_3:43991035-43991054 None:intergenic 25.0%
!!! GTTTTTAATTGGAATGTTGA+TGG - chr8_3:43991037-43991056 Msa1289700:intron 25.0%
! TGAAAGCAGAAAAAATTAAC+AGG + chr8_3:43991155-43991174 None:intergenic 25.0%
! GAAAGGAAATTACTAAAAGT+AGG - chr8_3:43991226-43991245 Msa1289700:CDS 25.0%
! ATTATAACTGACCAACAATT+AGG + chr8_3:43991300-43991319 None:intergenic 25.0%
! ATTGAGACTTTCTTCAATTT+AGG - chr8_3:43991344-43991363 Msa1289700:CDS 25.0%
!! CTTTCTTCAATTTAGGTTAT+TGG - chr8_3:43991351-43991370 Msa1289700:CDS 25.0%
!! TGTTTAACACTTACATCTTT+TGG + chr8_3:43991592-43991611 None:intergenic 25.0%
! TGAACATTGGATTAATCTAA+GGG + chr8_3:43991977-43991996 None:intergenic 25.0%
! TTGAACATTGGATTAATCTA+AGG + chr8_3:43991978-43991997 None:intergenic 25.0%
! GATAAATATACTTGATTGAG+AGG - chr8_3:43992021-43992040 Msa1289700:intron 25.0%
! GAGGATACAGATTAAAAATT+TGG - chr8_3:43992040-43992059 Msa1289700:intron 25.0%
!! CATTTTATTATTACACGATG+AGG + chr8_3:43992305-43992324 None:intergenic 25.0%
!!! TATAATATAGGAGCATTTGA+AGG + chr8_3:43992354-43992373 None:intergenic 25.0%
!!! CAGGCTCTTTTTTTTTTTTT+TGG - chr8_3:43992543-43992562 Msa1289700:five_prime_UTR 25.0%
!!! CTCTCTCTAGTTTTTTCTTT+CGG - chr8_3:43989368-43989387 Msa1289700:three_prime_UTR 30.0%
!! TATTTTGGGGAGTGATTAAT+TGG - chr8_3:43989943-43989962 Msa1289700:intron 30.0%
AAATGAAGCAAAATCTTGAG+CGG + chr8_3:43990112-43990131 None:intergenic 30.0%
! TTCACAAACTATTTTGCCTA+TGG - chr8_3:43990190-43990209 Msa1289700:intron 30.0%
! CAACATAAGGACCAAAATTT+TGG - chr8_3:43990317-43990336 Msa1289700:intron 30.0%
GTCATAAAACGGTAGATAAT+TGG + chr8_3:43990364-43990383 None:intergenic 30.0%
AATGGTTGTCCTAATTATTC+TGG - chr8_3:43990426-43990445 Msa1289700:intron 30.0%
AATTAGTCGGTGTGATTTAA+TGG - chr8_3:43990507-43990526 Msa1289700:intron 30.0%
! AGAATGATAGTGATGGAAAA+CGG - chr8_3:43990553-43990572 Msa1289700:CDS 30.0%
ACTTCTCAATATCAAGAGTT+AGG - chr8_3:43990932-43990951 Msa1289700:intron 30.0%
!!! TCTATGCCTCTGTTTTTAAT+TGG - chr8_3:43991026-43991045 Msa1289700:intron 30.0%
! AATGTTGATGGACAATAAAC+AGG - chr8_3:43991049-43991068 Msa1289700:intron 30.0%
TGAAAGGATGAAAATTCTAC+AGG - chr8_3:43991091-43991110 Msa1289700:intron 30.0%
! TTTAGTTCCTGGTTGTAATA+AGG - chr8_3:43991115-43991134 Msa1289700:intron 30.0%
AACATTACCTTATTACAACC+AGG + chr8_3:43991125-43991144 None:intergenic 30.0%
GTTATGATCTTCCTAATTGT+TGG - chr8_3:43991286-43991305 Msa1289700:CDS 30.0%
GTTAATTCAAGACTCAATAC+CGG - chr8_3:43991550-43991569 Msa1289700:intron 30.0%
! TTAACACTTACATCTTTTGG+AGG + chr8_3:43991589-43991608 None:intergenic 30.0%
TGTTGCAAGTATGTATAACA+AGG - chr8_3:43991741-43991760 Msa1289700:intron 30.0%
TGAATAATGTCTGCAAAAGA+TGG + chr8_3:43991772-43991791 None:intergenic 30.0%
AACAGCTAAAGATTGAACAT+TGG + chr8_3:43991990-43992009 None:intergenic 30.0%
TGGAAAGAGAGAAAAAAACT+AGG + chr8_3:43992140-43992159 None:intergenic 30.0%
!!! TTTTTTCTCTCTTTCCAGTA+TGG - chr8_3:43992143-43992162 Msa1289700:CDS 30.0%
CATACCTTTGTGTAGAAAAT+GGG + chr8_3:43992430-43992449 None:intergenic 30.0%
CTCAATATTGTGTACTATAG+TGG - chr8_3:43992515-43992534 Msa1289700:five_prime_UTR 30.0%
!! ATGTTGAGTTGAAAACGGTT+CGG - chr8_3:43989463-43989482 Msa1289700:three_prime_UTR 35.0%
TTGAAGTTAGTGAACCGAAA+TGG - chr8_3:43989493-43989512 Msa1289700:three_prime_UTR 35.0%
TTCAAAACAACTCTTGACTC+CGG + chr8_3:43989719-43989738 None:intergenic 35.0%
!!! TCAAGAGTTGTTTTGAACCA+TGG - chr8_3:43989722-43989741 Msa1289700:CDS 35.0%
AAAAATGAATACCACGGCAT+TGG + chr8_3:43989825-43989844 None:intergenic 35.0%
AATGAAGCAAAATCTTGAGC+GGG + chr8_3:43990111-43990130 None:intergenic 35.0%
! GTTTGAGATTACACTTCCAT+AGG + chr8_3:43990209-43990228 None:intergenic 35.0%
TTCGAACTTCGATGTCTAAA+TGG - chr8_3:43990408-43990427 Msa1289700:CDS 35.0%
! ATTGTGCAGAATGATAGTGA+TGG - chr8_3:43990546-43990565 Msa1289700:CDS 35.0%
ACATGGATGTAATCTTGCTT+AGG + chr8_3:43990687-43990706 None:intergenic 35.0%
GAAGGGAAAAGATTAGTGAA+AGG - chr8_3:43991075-43991094 Msa1289700:intron 35.0%
! ATTCTACAGGATTTAGTTCC+TGG - chr8_3:43991104-43991123 Msa1289700:intron 35.0%
! TTAGGTTATTGGGAAAGCAT+TGG - chr8_3:43991362-43991381 Msa1289700:CDS 35.0%
TTAGAATAGATACCTCCACT+TGG + chr8_3:43991428-43991447 None:intergenic 35.0%
!! ACTTACATCTTTTGGAGGAA+AGG + chr8_3:43991584-43991603 None:intergenic 35.0%
! ATTTTCCTTCTGACAAGACA+TGG - chr8_3:43991678-43991697 Msa1289700:intron 35.0%
ATGAATGATAGCTTCTCACT+AGG - chr8_3:43991873-43991892 Msa1289700:intron 35.0%
! CTTGTAGCTTGTGAAACAAA+TGG + chr8_3:43992197-43992216 None:intergenic 35.0%
! CTTAAGGTGTTAAAGTCCAA+TGG - chr8_3:43992390-43992409 Msa1289700:CDS 35.0%
! CATTCCCATTTTCTACACAA+AGG - chr8_3:43992423-43992442 Msa1289700:CDS 35.0%
CCATACCTTTGTGTAGAAAA+TGG + chr8_3:43992431-43992450 None:intergenic 35.0%
! CCATTTTCTACACAAAGGTA+TGG - chr8_3:43992428-43992447 Msa1289700:exon 35.0%
GTCGTATTTCCATTCCCATT+CGG + chr8_3:43989551-43989570 None:intergenic 40.0%
CAGATCTCCGCTAAACTAAA+CGG + chr8_3:43989575-43989594 None:intergenic 40.0%
! TTAGGACAGTTTGGTGAGTT+TGG - chr8_3:43989665-43989684 Msa1289700:exon 40.0%
ATTACCATCCCGGTTTAAAC+CGG + chr8_3:43989693-43989712 None:intergenic 40.0%
!!! AGAGTTGTTTTGAACCATGG+TGG - chr8_3:43989725-43989744 Msa1289700:CDS 40.0%
TTACTGGTGGAAACACACTT+AGG + chr8_3:43989794-43989813 None:intergenic 40.0%
AAGTGTGTTTCCACCAGTAA+TGG - chr8_3:43989794-43989813 Msa1289700:intron 40.0%
CAAGTTTGGTTGCGATTTCA+AGG - chr8_3:43990276-43990295 Msa1289700:intron 40.0%
GATGCCTTTGCTACAACATA+AGG - chr8_3:43990304-43990323 Msa1289700:intron 40.0%
TGGTCCTTATGTTGTAGCAA+AGG + chr8_3:43990311-43990330 None:intergenic 40.0%
! GAGCGAAGTAGCCAAAATTT+TGG + chr8_3:43990331-43990350 None:intergenic 40.0%
!! GAAAACGGACTAAAGCATCA+GGG - chr8_3:43990568-43990587 Msa1289700:CDS 40.0%
GGATGTAATCTTGCTTAGGA+GGG + chr8_3:43990683-43990702 None:intergenic 40.0%
TGGATGTAATCTTGCTTAGG+AGG + chr8_3:43990684-43990703 None:intergenic 40.0%
TGGACAATAAACAGGCTAGA+AGG - chr8_3:43991057-43991076 Msa1289700:intron 40.0%
GGACAATAAACAGGCTAGAA+GGG - chr8_3:43991058-43991077 Msa1289700:intron 40.0%
! AAGTAGGAAAACGTGCTACT+TGG - chr8_3:43991242-43991261 Msa1289700:CDS 40.0%
!! AGTAGGAAAACGTGCTACTT+GGG - chr8_3:43991243-43991262 Msa1289700:CDS 40.0%
ACTCACGAGTATAGTAGTTC+TGG - chr8_3:43991709-43991728 Msa1289700:intron 40.0%
TTTAAGAAGCTTGCAACCTC+TGG - chr8_3:43991916-43991935 Msa1289700:intron 40.0%
CTTTAGCTGTTTAGCTGTAG+AGG - chr8_3:43991999-43992018 Msa1289700:intron 40.0%
! CAAACGTTTGTTGACCATAC+TGG + chr8_3:43992160-43992179 None:intergenic 40.0%
CAACAAACGTTTGATCTTGC+CGG - chr8_3:43992167-43992186 Msa1289700:CDS 40.0%
TTGTGAAACAAATGGCATGC+CGG + chr8_3:43992189-43992208 None:intergenic 40.0%
ACCAGAATGGTTACATATGC+AGG - chr8_3:43992238-43992257 Msa1289700:CDS 40.0%
ACCTGCATATGTAACCATTC+TGG + chr8_3:43992242-43992261 None:intergenic 40.0%
GAATGGTTACATATGCAGGT+AGG - chr8_3:43992242-43992261 Msa1289700:CDS 40.0%
! GGAGCATTTGAAGGATGAAA+TGG + chr8_3:43992345-43992364 None:intergenic 40.0%
!! GGAATGAAGAAAGTGTCCAT+TGG + chr8_3:43992409-43992428 None:intergenic 40.0%
! GAGCTGTTACTGTTTTCAAC+AGG - chr8_3:43992450-43992469 Msa1289700:five_prime_UTR 40.0%
!! GTGTACTATAGTGGACTTTC+AGG - chr8_3:43992524-43992543 Msa1289700:five_prime_UTR 40.0%
TCGTGTTTGTTTGTGTAGCA+AGG - chr8_3:43992664-43992683 Msa1289700:five_prime_UTR 40.0%
!! GAGAGAGAGAGTGTTAGGTT+CGG + chr8_3:43989327-43989346 None:intergenic 45.0%
!! AGAGAGAGAGAGAGAGTGTT+AGG + chr8_3:43989332-43989351 None:intergenic 45.0%
!! GAGTTGAAAACGGTTCGGTT+CGG - chr8_3:43989468-43989487 Msa1289700:three_prime_UTR 45.0%
!! AGTTGAAAACGGTTCGGTTC+GGG - chr8_3:43989469-43989488 Msa1289700:three_prime_UTR 45.0%
TAGTGAACCGAAATGGATCC+AGG - chr8_3:43989500-43989519 Msa1289700:three_prime_UTR 45.0%
AACGAGGGTTCATTTCCGAA+TGG - chr8_3:43989533-43989552 Msa1289700:three_prime_UTR 45.0%
ACGAGGGTTCATTTCCGAAT+GGG - chr8_3:43989534-43989553 Msa1289700:three_prime_UTR 45.0%
GGTTCATTTCCGAATGGGAA+TGG - chr8_3:43989539-43989558 Msa1289700:three_prime_UTR 45.0%
! TACGACGCCGTTTAGTTTAG+CGG - chr8_3:43989565-43989584 Msa1289700:three_prime_UTR 45.0%
! CGTTTAGTTTAGCGGAGATC+TGG - chr8_3:43989573-43989592 Msa1289700:three_prime_UTR 45.0%
!! GAGGTGGATTAGGACTAAGA+AGG - chr8_3:43989624-43989643 Msa1289700:three_prime_UTR 45.0%
AGTTTGGACCCGGTTTAAAC+CGG - chr8_3:43989681-43989700 Msa1289700:CDS 45.0%
TTACCATCCCGGTTTAAACC+GGG + chr8_3:43989692-43989711 None:intergenic 45.0%
GGTTTAAACCGGGATGGTAA+TGG - chr8_3:43989692-43989711 Msa1289700:CDS 45.0%
!! GTTTTGAACCATGGTGGTGT+TGG - chr8_3:43989731-43989750 Msa1289700:CDS 45.0%
TGTGTTTCCACCAGTAATGG+CGG - chr8_3:43989797-43989816 Msa1289700:intron 45.0%
!! GGAAAACGGACTAAAGCATC+AGG - chr8_3:43990567-43990586 Msa1289700:CDS 45.0%
AGCATCAGGGAACAAGAATG+AGG - chr8_3:43990581-43990600 Msa1289700:intron 45.0%
GCATCAGGGAACAAGAATGA+GGG - chr8_3:43990582-43990601 Msa1289700:intron 45.0%
AACAAGAATGAGGGTGGTGA+TGG - chr8_3:43990591-43990610 Msa1289700:intron 45.0%
!! GGTGGTGATGGTAAAGTTGA+AGG - chr8_3:43990603-43990622 Msa1289700:intron 45.0%
GTTGAAGGAGAAGTCAGTTC+AGG - chr8_3:43990618-43990637 Msa1289700:intron 45.0%
! AGTCAGTTCAGGAAAGCATG+CGG - chr8_3:43990629-43990648 Msa1289700:intron 45.0%
ACATCCATGTTCGAGCTAGA+AGG - chr8_3:43990697-43990716 Msa1289700:intron 45.0%
CATCCATGTTCGAGCTAGAA+GGG - chr8_3:43990698-43990717 Msa1289700:intron 45.0%
ATCCATGTTCGAGCTAGAAG+GGG - chr8_3:43990699-43990718 Msa1289700:intron 45.0%
AACCTCTGGTCTCTGCATAA+TGG - chr8_3:43991930-43991949 Msa1289700:intron 45.0%
ACCTCTGGTCTCTGCATAAT+GGG - chr8_3:43991931-43991950 Msa1289700:intron 45.0%
TGGTTACATATGCAGGTAGG+TGG - chr8_3:43992245-43992264 Msa1289700:CDS 45.0%
TTACATATGCAGGTAGGTGG+TGG - chr8_3:43992248-43992267 Msa1289700:CDS 45.0%
!!! AATATTAAATATATGTATTT+TGG - chr8_3:43989928-43989947 Msa1289700:intron 5.0%
!!! ATATTAAATATATGTATTTT+GGG - chr8_3:43989929-43989948 Msa1289700:intron 5.0%
TCTCCTTGGACAGTGGAGAA+TGG + chr8_3:43989297-43989316 None:intergenic 50.0%
CATCGCTCCTGGATCCATTT+CGG + chr8_3:43989510-43989529 None:intergenic 50.0%
CAGTTTCCAGCGGCGATTAA+TGG - chr8_3:43989596-43989615 Msa1289700:three_prime_UTR 50.0%
TTTCCAGCGGCGATTAATGG+CGG - chr8_3:43989599-43989618 Msa1289700:three_prime_UTR 50.0%
GACTAAGAAGGCCGCAGTTT+GGG - chr8_3:43989636-43989655 Msa1289700:three_prime_UTR 50.0%
CCTAAACCGTTCCCAAACTG+CGG + chr8_3:43989650-43989669 None:intergenic 50.0%
! GGGAACGGTTTAGGACAGTT+TGG - chr8_3:43989656-43989675 Msa1289700:exon 50.0%
! CAGTTTGGTGAGTTTGGACC+CGG - chr8_3:43989671-43989690 Msa1289700:CDS 50.0%
GTTTGGACCCGGTTTAAACC+GGG - chr8_3:43989682-43989701 Msa1289700:CDS 50.0%
!! AAACCGGGATGGTAATGGAC+CGG - chr8_3:43989697-43989716 Msa1289700:CDS 50.0%
! TTGAACCATGGTGGTGTTGG+TGG - chr8_3:43989734-43989753 Msa1289700:CDS 50.0%
!! GTGTTGGTGGTGGTAAGAAG+AGG - chr8_3:43989747-43989766 Msa1289700:CDS 50.0%
TCAGGGAACAAGAATGAGGG+TGG - chr8_3:43990585-43990604 Msa1289700:intron 50.0%
TCTTGCTTAGGAGGGTCAGA+AGG + chr8_3:43990675-43990694 None:intergenic 50.0%
TTACGCGTTCAGCAAGACTG+TGG + chr8_3:43990740-43990759 None:intergenic 50.0%
!! TGAGTTGAGAAGCGCTGTTG+TGG - chr8_3:43990865-43990884 Msa1289700:CDS 50.0%
TCAATCACTTCAGCGCCAAG+TGG - chr8_3:43991410-43991429 Msa1289700:intron 50.0%
GAGAGCCATGTCTTGTCAGA+AGG + chr8_3:43991686-43991705 None:intergenic 50.0%
TCTGGTGAAGAACCTCTGCT+TGG + chr8_3:43992224-43992243 None:intergenic 50.0%
GCAGAGGTTCTTCACCAGAA+TGG - chr8_3:43992225-43992244 Msa1289700:CDS 50.0%
CAACAGGCTGCTTCAGTTTG+TGG - chr8_3:43992466-43992485 Msa1289700:five_prime_UTR 50.0%
GCACCATTCTCCACTGTCCA+AGG - chr8_3:43989291-43989310 Msa1289700:three_prime_UTR 55.0%
TGCGTGATCTCCTTGGACAG+TGG + chr8_3:43989304-43989323 None:intergenic 55.0%
TCCAGGAGCGATGATGAACG+AGG - chr8_3:43989517-43989536 Msa1289700:three_prime_UTR 55.0%
CCAGGAGCGATGATGAACGA+GGG - chr8_3:43989518-43989537 Msa1289700:three_prime_UTR 55.0%
GGAGATCTGGCAGTTTCCAG+CGG - chr8_3:43989586-43989605 Msa1289700:three_prime_UTR 55.0%
GGACTAAGAAGGCCGCAGTT+TGG - chr8_3:43989635-43989654 Msa1289700:three_prime_UTR 55.0%
AGAAGGCCGCAGTTTGGGAA+CGG - chr8_3:43989641-43989660 Msa1289700:three_prime_UTR 55.0%
CCGCAGTTTGGGAACGGTTT+AGG - chr8_3:43989647-43989666 Msa1289700:three_prime_UTR 55.0%
ACTCCGGTCCATTACCATCC+CGG + chr8_3:43989703-43989722 None:intergenic 55.0%
!! AACCATGGTGGTGTTGGTGG+TGG - chr8_3:43989737-43989756 Msa1289700:CDS 55.0%
TACCACCACCAACACCACCA+TGG + chr8_3:43989742-43989761 None:intergenic 55.0%
ATTGGCACCGCCATTACTGG+TGG + chr8_3:43989807-43989826 None:intergenic 55.0%
TTAGGAGGGTCAGAAGGAGG+TGG + chr8_3:43990669-43990688 None:intergenic 55.0%
TGCTTAGGAGGGTCAGAAGG+AGG + chr8_3:43990672-43990691 None:intergenic 55.0%
!! GACCCCTTCTAGCTCGAACA+TGG + chr8_3:43990704-43990723 None:intergenic 55.0%
TTGTGGCTGCACGACAGCAT+AGG - chr8_3:43990882-43990901 Msa1289700:intron 55.0%
ATCACTTCAGCGCCAAGTGG+AGG - chr8_3:43991413-43991432 Msa1289700:intron 55.0%
TGGAGGAAAGGCTTCGATGC+CGG + chr8_3:43991572-43991591 None:intergenic 55.0%
GCCCATTATGCAGAGACCAG+AGG + chr8_3:43991935-43991954 None:intergenic 55.0%
GCTACAAGAGAGCCAAGCAG+AGG - chr8_3:43992209-43992228 Msa1289700:CDS 55.0%
!! GGTTCGGTGCGTGATCTCCT+TGG + chr8_3:43989311-43989330 None:intergenic 60.0%
CCCTCGTTCATCATCGCTCC+TGG + chr8_3:43989521-43989540 None:intergenic 60.0%
! AATGGCGGCGGAGGTGGATT+AGG - chr8_3:43989614-43989633 Msa1289700:three_prime_UTR 60.0%
GGACCCGGTTTAAACCGGGA+TGG - chr8_3:43989686-43989705 Msa1289700:CDS 60.0%
!! GGCATTGGCACCGCCATTAC+TGG + chr8_3:43989810-43989829 None:intergenic 60.0%
! TAATGGCGGTGCCAATGCCG+TGG - chr8_3:43989811-43989830 Msa1289700:intron 60.0%
CCAGCGGCGATTAATGGCGG+CGG - chr8_3:43989602-43989621 Msa1289700:three_prime_UTR 65.0%
GCGATTAATGGCGGCGGAGG+TGG - chr8_3:43989608-43989627 Msa1289700:three_prime_UTR 65.0%
CCGCCGCCATTAATCGCCGC+TGG + chr8_3:43989605-43989624 None:intergenic 70.0%
GCGGCGATTAATGGCGGCGG+AGG - chr8_3:43989605-43989624 Msa1289700:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 43989239 43992719 43989239 ID=Msa1289700;Name=Msa1289700
chr8_3 mRNA 43989239 43992719 43989239 ID=Msa1289700-mRNA-1;Parent=Msa1289700;Name=Msa1289700-mRNA-1;_AED=0.02;_eAED=0.02;_QI=273|1|1|1|1|1|6|430|285
chr8_3 exon 43989239 43989797 43989239 ID=Msa1289700-mRNA-1:exon:12065;Parent=Msa1289700-mRNA-1
chr8_3 exon 43990361 43990432 43990361 ID=Msa1289700-mRNA-1:exon:12064;Parent=Msa1289700-mRNA-1
chr8_3 exon 43990524 43990592 43990524 ID=Msa1289700-mRNA-1:exon:12063;Parent=Msa1289700-mRNA-1
chr8_3 exon 43990822 43990887 43990822 ID=Msa1289700-mRNA-1:exon:12062;Parent=Msa1289700-mRNA-1
chr8_3 exon 43991203 43991403 43991203 ID=Msa1289700-mRNA-1:exon:12061;Parent=Msa1289700-mRNA-1
chr8_3 exon 43992126 43992719 43992126 ID=Msa1289700-mRNA-1:exon:12060;Parent=Msa1289700-mRNA-1
chr8_3 five_prime_UTR 43992447 43992719 43992447 ID=Msa1289700-mRNA-1:five_prime_utr;Parent=Msa1289700-mRNA-1
chr8_3 CDS 43992126 43992446 43992126 ID=Msa1289700-mRNA-1:cds;Parent=Msa1289700-mRNA-1
chr8_3 CDS 43991203 43991403 43991203 ID=Msa1289700-mRNA-1:cds;Parent=Msa1289700-mRNA-1
chr8_3 CDS 43990822 43990887 43990822 ID=Msa1289700-mRNA-1:cds;Parent=Msa1289700-mRNA-1
chr8_3 CDS 43990524 43990592 43990524 ID=Msa1289700-mRNA-1:cds;Parent=Msa1289700-mRNA-1
chr8_3 CDS 43990361 43990432 43990361 ID=Msa1289700-mRNA-1:cds;Parent=Msa1289700-mRNA-1
chr8_3 CDS 43989669 43989797 43989669 ID=Msa1289700-mRNA-1:cds;Parent=Msa1289700-mRNA-1
chr8_3 three_prime_UTR 43989239 43989668 43989239 ID=Msa1289700-mRNA-1:three_prime_utr;Parent=Msa1289700-mRNA-1
Gene Sequence

>Msa1289700

ATGGATCCAGGAGCGATGATGAACGAGGGTTCATTTCCGAATGGGAATGGAAATACGACGCCGTTTAGTTTAGCGGAGATCTGGCAGTTTCCAGCGGCGATTAATGGCGGCGGAGGTGGATTAGGACTAAGAAGGCCGCAGTTTGGGAACGGTTTAGGACAGTTTGGTGAGTTTGGACCCGGTTTAAACCGGGATGGTAATGGACCGGAGTCAAGAGTTGTTTTGAACCATGGTGGTGTTGGTGGTGGTAAGAAGAGGCGTGACTCTGAAGATGATTCTCCTAAGTGTGTTTCCACCAGTAATGGCGGTGCCAATGCCGTGAATGATAGTGATGGAAAACGGACTAAAGCATCAGGGAACAAGAATGAGGGTGGTGATGGTAAAGTTGAAGGAGAAGTCAGTTCAGGAAAGCATGCGGAGCAAAGCAGTAAACCACCTCCTTCTGACCCTCCTAAGCAAGATTACATCCATGTTCGAGCTAGAAGGGGTCAAGCTACTGATAGCCACAGTCTTGCTGAACGCGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAATTCTACAGGATTTAGTTCCTGGTTGTAATAAGGTTATTGGGAAAGCATTGGTGCTTGATGAGATAATTAATTATATTCAATCACTTCAGCGCCAAGTGGAGTTTTTATCGATGAAACTTGAAGCAGTTAATTCAAGACTCAATACCGGCATCGAAGCCTTTCCTCCAAAAGATTATGGTCAACAAACGTTTGATCTTGCCGGCATGCCATTTGTTTCACAAGCTACAAGAGAGCCAAGCAGAGGTTCTTCACCAGAATGGTTACATATGCAGGTAGGTGGTGGTTTCGAAAGAACGACGTAG
Protein sequence

>Msa1289700

MDPGAMMNEGSFPNGNGNTTPFSLAEIWQFPAAINGGGGGLGLRRPQFGNGLGQFGEFGPGLNRDGNGPESRVVLNHGGVGGGKKRRDSEDDSPKCVSTSNGGANAVNDSDGKRTKASGNKNEGGDGKVEGEVSSGKHAEQSSKPPPSDPPKQDYIHVRARRGQATDSHSLAERARREKISERMKILQDLVPGCNKVIGKALVLDEIINYIQSLQRQVEFLSMKLEAVNSRLNTGIEAFPPKDYGQQTFDLAGMPFVSQATREPSRGSSPEWLHMQVGGGFERTT*