AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1289790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1289790 MtrunA17_Chr8g0364021 88.591 149 13 3 1 146 31 178 3.48e-90 262
Msa1289790 MtrunA17_Chr5g0415031 55.172 87 39 0 4 90 33 119 5.54e-28 108
Msa1289790 MtrunA17_Chr8g0364231 55.172 87 39 0 6 92 34 120 6.05e-27 105
Msa1289790 MtrunA17_Chr6g0452001 49.515 103 48 2 2 103 54 153 3.29e-23 94.7
Msa1289790 MtrunA17_Chr6g0452021 47.917 96 49 1 8 103 37 131 7.24e-22 90.5
Msa1289790 MtrunA17_Chr1g0206781 47.191 89 46 1 8 96 5 92 2.79e-20 80.5
Msa1289790 MtrunA17_Chr1g0206771 51.389 72 35 0 7 78 9 80 1.46e-18 77.8
Msa1289790 MtrunA17_Chr1g0206771 45.000 80 44 0 13 92 94 173 4.87e-13 63.2
Msa1289790 MtrunA17_Chr3g0077891 49.383 81 40 1 5 84 90 170 1.42e-17 75.1
Msa1289790 MtrunA17_Chr3g0077891 50.649 77 37 1 8 83 2 78 4.77e-16 71.2
Msa1289790 MtrunA17_Chr3g0077881 43.119 109 55 3 8 109 2 110 3.18e-17 74.3
Msa1289790 MtrunA17_Chr3g0077881 42.391 92 45 2 5 95 90 174 9.33e-13 62.4
Msa1289790 MtrunA17_Chr3g0077941 50.649 77 37 1 8 83 2 78 3.23e-16 71.6
Msa1289790 MtrunA17_Chr3g0077941 48.684 76 38 1 7 81 91 166 2.73e-15 69.3
Msa1289790 MtrunA17_Chr3g0077911 50.000 74 36 1 7 79 83 156 3.52e-16 71.2
Msa1289790 MtrunA17_Chr3g0077911 41.379 87 46 2 8 92 2 85 6.20e-12 60.1
Msa1289790 MtrunA17_Chr1g0205811 41.573 89 52 0 1 89 1 89 7.04e-16 69.3
Msa1289790 MtrunA17_Chr1g0205821 38.636 88 54 0 5 92 90 177 1.15e-15 70.1
Msa1289790 MtrunA17_Chr1g0205821 38.372 86 53 0 13 98 12 97 2.40e-12 61.2
Msa1289790 MtrunA17_Chr3g0077861 48.649 74 37 1 7 79 83 156 1.58e-15 69.7
Msa1289790 MtrunA17_Chr3g0077861 41.573 89 48 2 8 95 2 87 2.61e-11 58.5
Msa1289790 MtrunA17_Chr4g0075861 43.373 83 45 1 9 89 84 166 5.72e-14 67.8
Msa1289790 MtrunA17_Chr4g0075861 45.455 77 40 1 7 83 167 241 2.80e-12 63.2
Msa1289790 MtrunA17_Chr4g0075861 45.000 80 41 1 13 92 11 87 1.46e-11 60.8
Msa1289790 MtrunA17_Chr1g0205861 36.364 110 67 2 6 113 8 116 6.48e-14 64.3
Msa1289790 MtrunA17_Chr7g0218911 39.394 66 40 0 18 83 2 67 4.65e-13 62.0
Msa1289790 MtrunA17_Chr4g0075971 40.964 83 47 1 9 89 84 166 3.14e-12 63.2
Msa1289790 MtrunA17_Chr4g0075971 45.000 80 41 1 13 92 11 87 1.90e-11 60.8
Msa1289790 MtrunA17_Chr4g0075971 43.750 80 43 1 7 86 167 244 2.63e-11 60.5
Msa1289790 MtrunA17_Chr2g0328701 38.947 95 51 2 13 107 28 115 9.38e-12 58.9
Msa1289790 MtrunA17_Chr1g0205801 38.158 76 47 0 14 89 12 87 3.21e-11 57.0
Msa1289790 MtrunA17_Chr7g0218891 39.744 78 45 1 14 89 185 262 9.89e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1289790 AT1G24190.3 52.941 85 40 0 6 90 6 90 2.68e-24 98.2
Msa1289790 AT1G24190.2 52.941 85 40 0 6 90 6 90 2.87e-24 98.2
Msa1289790 AT1G24190.1 52.941 85 40 0 6 90 6 90 2.87e-24 98.2
Msa1289790 AT3G01320.1 52.439 82 39 0 7 88 50 131 1.88e-23 95.9
Msa1289790 AT3G01320.2 52.439 82 39 0 7 88 50 131 1.90e-23 95.9
Msa1289790 AT1G70060.2 48.889 90 46 0 1 90 1 90 1.91e-23 95.9
Msa1289790 AT1G70060.1 48.889 90 46 0 1 90 1 90 1.91e-23 95.9
Msa1289790 AT1G59890.3 50.000 82 41 0 14 95 45 126 1.65e-22 93.2
Msa1289790 AT1G59890.2 50.000 82 41 0 14 95 45 126 1.65e-22 93.2
Msa1289790 AT1G59890.1 50.000 82 41 0 14 95 45 126 1.68e-22 93.2
Msa1289790 AT1G59890.4 50.000 82 41 0 14 95 45 126 1.75e-22 93.2
Msa1289790 AT5G15025.1 51.899 79 38 0 10 88 43 121 9.33e-22 84.7
Msa1289790 AT5G15020.1 45.833 96 51 1 8 103 46 140 2.13e-21 90.1
Msa1289790 AT5G15020.2 45.833 96 51 1 8 103 46 140 2.25e-21 89.7
Msa1289790 AT1G10450.3 46.341 82 44 0 14 95 84 165 5.60e-19 82.8
Msa1289790 AT1G10450.2 46.341 82 44 0 14 95 33 114 6.33e-19 82.8
Msa1289790 AT1G10450.1 46.341 82 44 0 14 95 33 114 6.33e-19 82.8
Msa1289790 AT1G70030.3 43.529 85 48 0 3 87 11 95 7.32e-19 77.4
Msa1289790 AT1G70030.2 43.529 85 48 0 3 87 11 95 1.34e-18 77.4
Msa1289790 AT1G70030.1 43.902 82 46 0 6 87 3 84 4.34e-18 76.6
Msa1289790 AT5G15040.1 45.333 75 41 0 17 91 6 80 1.63e-17 72.8
Msa1289790 AT5G15040.3 45.333 75 41 0 17 91 6 80 1.63e-17 72.8
Msa1289790 AT5G15040.2 45.333 75 41 0 17 91 6 80 1.63e-17 72.8
Msa1289790 AT1G27220.1 42.553 94 47 2 5 91 4 97 3.10e-17 74.7
Msa1289790 AT4G12020.6 44.944 89 48 1 10 98 304 391 5.56e-17 77.4
Msa1289790 AT4G12020.8 44.944 89 48 1 10 98 304 391 5.56e-17 77.4
Msa1289790 AT4G12020.4 44.944 89 48 1 10 98 304 391 5.57e-17 77.4
Msa1289790 AT4G12020.7 44.944 89 48 1 10 98 304 391 5.61e-17 77.4
Msa1289790 AT4G12020.2 44.944 89 48 1 10 98 304 391 5.68e-17 77.4
Msa1289790 AT4G12020.5 44.944 89 48 1 10 98 237 324 5.73e-17 77.4
Msa1289790 AT4G12020.3 44.944 89 48 1 10 98 304 391 5.74e-17 77.4
Msa1289790 AT4G12020.1 44.944 89 48 1 10 98 304 391 5.84e-17 77.4
Msa1289790 AT4G12020.9 44.944 89 48 1 10 98 304 391 5.84e-17 77.4
Msa1289790 AT4G12020.10 44.944 89 48 1 10 98 304 391 6.14e-17 77.0
Msa1289790 AT4G12020.11 44.944 89 48 1 10 98 304 391 6.16e-17 77.0
Msa1289790 AT1G24200.1 44.944 89 49 0 1 89 1 89 8.09e-17 73.9
Msa1289790 AT1G27250.1 42.466 73 42 0 9 81 16 88 1.20e-15 69.7
Msa1289790 AT1G24210.1 39.744 78 42 1 10 87 10 82 7.45e-14 65.5
Msa1289790 AT1G27260.1 41.429 70 41 0 15 84 4 73 7.35e-13 63.9
Msa1289790 AT5G35610.1 38.144 97 59 1 6 102 2 97 2.36e-12 61.2
Msa1289790 AT1G23810.1 42.188 64 37 0 11 74 106 169 2.13e-11 60.1
Msa1289790 AT1G24250.1 42.188 64 37 0 11 74 117 180 5.61e-11 59.3

Find 36 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCATCTTCTTCTGAAAT+TGG 0.145375 8_3:+44238918 None:intergenic
GTTCATCTTCTTCTGAAATT+GGG 0.296314 8_3:+44238919 None:intergenic
TGATGCCTTTGCATATCTTA+AGG 0.401654 8_3:-44239401 Msa1289790:CDS
ACAAACTCATTAGCGTGTTC+AGG 0.404504 8_3:+44238759 None:intergenic
TGAAGGATTACAAAGATAAC+AGG 0.410405 8_3:-44239321 Msa1289790:intron
AGGACATAATGATCTGATTA+CGG 0.410966 8_3:-44238976 Msa1289790:CDS
CCAAATGGTTCATTCCTTGA+TGG 0.420352 8_3:+44238786 None:intergenic
GGATGTCTTCAGAGAACAAA+TGG 0.428268 8_3:-44239371 Msa1289790:CDS
CACATTCATCAAGATTCCTA+AGG 0.446532 8_3:+44238892 None:intergenic
GGACATAATGATCTGATTAC+GGG 0.453739 8_3:-44238975 Msa1289790:CDS
AACACGCTAATGAGTTTGTC+AGG 0.476933 8_3:-44238755 Msa1289790:CDS
CCATCAAGGAATGAACCATT+TGG 0.482429 8_3:-44238786 Msa1289790:CDS
TGAGTTTGTCAGGAAAGTTA+AGG 0.495626 8_3:-44238745 Msa1289790:CDS
GCGTGTTCAGGTCCACCAAA+TGG 0.497913 8_3:+44238771 None:intergenic
AAGAAGATGAACAACCCCTT+AGG 0.498111 8_3:-44238908 Msa1289790:CDS
ATATGCAAAGGCATCATCTT+CGG 0.504699 8_3:+44239408 None:intergenic
CAATCTCCTCAGATGATGCT+TGG 0.528479 8_3:-44238828 Msa1289790:CDS
ACATTCATCAAGATTCCTAA+GGG 0.529673 8_3:+44238893 None:intergenic
TCTCCTCAGATGATGCTTGG+TGG 0.532260 8_3:-44238825 Msa1289790:CDS
TGCATATCTTAAGGCAGTCA+AGG 0.543106 8_3:-44239392 Msa1289790:CDS
TAATTACCATGATGTTGTAA+CGG 0.566432 8_3:-44239018 Msa1289790:CDS
ATGTGAAATGACAATTCCAT+TGG 0.573688 8_3:-44238874 Msa1289790:CDS
TCAAGGAATGAACCATTTGG+TGG 0.574249 8_3:-44238783 Msa1289790:CDS
TGATGTTGTAACGGAGGTGA+AGG 0.575535 8_3:-44239009 Msa1289790:CDS
AGCATATCACAACTCCATCA+AGG 0.575744 8_3:-44238800 Msa1289790:CDS
TGAGGAGATTGTGCATTGAG+AGG 0.583914 8_3:+44238840 None:intergenic
TCACCTCCGTTACAACATCA+TGG 0.592553 8_3:+44239012 None:intergenic
TTCAACATCTTTGCACCAAG+TGG 0.595995 8_3:-44238951 Msa1289790:CDS
GAGGTGAAGGAGTTGTTCAA+AGG 0.606819 8_3:-44238996 Msa1289790:CDS
ACAAAGATGAGTACGAGTCA+AGG 0.614659 8_3:-44239445 None:intergenic
GACTGCCTTAAGATATGCAA+AGG 0.619589 8_3:+44239396 None:intergenic
ATTGGGATTTGATAACCACT+TGG 0.631213 8_3:+44238936 None:intergenic
TTACCATGATGTTGTAACGG+AGG 0.669596 8_3:-44239015 Msa1289790:CDS
TCTCCACCAAGCATCATCTG+AGG 0.679479 8_3:+44238822 None:intergenic
CATTCATCAAGATTCCTAAG+GGG 0.686349 8_3:+44238894 None:intergenic
AAGATGAGTACGAGTCAAGG+TGG 0.711763 8_3:-44239442 Msa1289790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACTTTTTGAAAATAATGA+AGG - chr8_3:44238826-44238845 Msa1289790:CDS 15.0%
!! TGGTAAACTATAAATAATAT+AGG + chr8_3:44239056-44239075 None:intergenic 15.0%
!! TAATTAGAGACGAAAAAAAT+TGG + chr8_3:44238997-44239016 None:intergenic 20.0%
!! AATAGAAAAATGTAAATTGC+CGG + chr8_3:44239023-44239042 None:intergenic 20.0%
! TATTTATAGTTTACCATGAG+AGG - chr8_3:44239060-44239079 Msa1289790:intron 25.0%
! TCGCAAATAAAATTAAGATC+CGG + chr8_3:44239086-44239105 None:intergenic 25.0%
! TAATTACCATGATGTTGTAA+CGG - chr8_3:44239146-44239165 Msa1289790:intron 25.0%
TGAAGGATTACAAAGATAAC+AGG - chr8_3:44238843-44238862 Msa1289790:CDS 30.0%
GATTACAAAGATAACAGGTT+AGG - chr8_3:44238848-44238867 Msa1289790:CDS 30.0%
CAATAGTGATTGAAAGAATG+TGG + chr8_3:44238891-44238910 None:intergenic 30.0%
!!! CATTTTTTCGTCCGAAATAT+TGG + chr8_3:44238951-44238970 None:intergenic 30.0%
! TTTTCGTCTCTAATTAATGC+CGG - chr8_3:44239001-44239020 Msa1289790:CDS 30.0%
AGGACATAATGATCTGATTA+CGG - chr8_3:44239188-44239207 Msa1289790:intron 30.0%
!! GTTCATCTTCTTCTGAAATT+GGG + chr8_3:44239248-44239267 None:intergenic 30.0%
!! TGTTCATCTTCTTCTGAAAT+TGG + chr8_3:44239249-44239268 None:intergenic 30.0%
ACATTCATCAAGATTCCTAA+GGG + chr8_3:44239274-44239293 None:intergenic 30.0%
ATGTGAAATGACAATTCCAT+TGG - chr8_3:44239290-44239309 Msa1289790:intron 30.0%
!!! AGAGGTTTTAATTCTTCCAA+TGG + chr8_3:44239309-44239328 None:intergenic 30.0%
!! ATATGCAAAGGCATCATCTT+CGG + chr8_3:44238759-44238778 None:intergenic 35.0%
TGATGCCTTTGCATATCTTA+AGG - chr8_3:44238763-44238782 Msa1289790:CDS 35.0%
CCAATCACTGACCAATATTT+CGG - chr8_3:44238937-44238956 Msa1289790:CDS 35.0%
GGACATAATGATCTGATTAC+GGG - chr8_3:44239189-44239208 Msa1289790:intron 35.0%
! ATTGGGATTTGATAACCACT+TGG + chr8_3:44239231-44239250 None:intergenic 35.0%
CATTCATCAAGATTCCTAAG+GGG + chr8_3:44239273-44239292 None:intergenic 35.0%
CACATTCATCAAGATTCCTA+AGG + chr8_3:44239275-44239294 None:intergenic 35.0%
TGAGTTTGTCAGGAAAGTTA+AGG - chr8_3:44239419-44239438 Msa1289790:CDS 35.0%
GACTGCCTTAAGATATGCAA+AGG + chr8_3:44238771-44238790 None:intergenic 40.0%
TGCATATCTTAAGGCAGTCA+AGG - chr8_3:44238772-44238791 Msa1289790:CDS 40.0%
GGATGTCTTCAGAGAACAAA+TGG - chr8_3:44238793-44238812 Msa1289790:CDS 40.0%
CCGAAATATTGGTCAGTGAT+TGG + chr8_3:44238940-44238959 None:intergenic 40.0%
TATAGTTTACCATGAGAGGC+CGG - chr8_3:44239064-44239083 Msa1289790:intron 40.0%
TTACCATGATGTTGTAACGG+AGG - chr8_3:44239149-44239168 Msa1289790:intron 40.0%
TTCAACATCTTTGCACCAAG+TGG - chr8_3:44239213-44239232 Msa1289790:intron 40.0%
AAGAAGATGAACAACCCCTT+AGG - chr8_3:44239256-44239275 Msa1289790:intron 40.0%
AGCATATCACAACTCCATCA+AGG - chr8_3:44239364-44239383 Msa1289790:CDS 40.0%
CCAAATGGTTCATTCCTTGA+TGG + chr8_3:44239381-44239400 None:intergenic 40.0%
CCATCAAGGAATGAACCATT+TGG - chr8_3:44239378-44239397 Msa1289790:CDS 40.0%
TCAAGGAATGAACCATTTGG+TGG - chr8_3:44239381-44239400 Msa1289790:CDS 40.0%
ACAAACTCATTAGCGTGTTC+AGG + chr8_3:44239408-44239427 None:intergenic 40.0%
AACACGCTAATGAGTTTGTC+AGG - chr8_3:44239409-44239428 Msa1289790:CDS 40.0%
AATTAAGATCCGGCCTCTCA+TGG + chr8_3:44239076-44239095 None:intergenic 45.0%
TCACCTCCGTTACAACATCA+TGG + chr8_3:44239155-44239174 None:intergenic 45.0%
! TGATGTTGTAACGGAGGTGA+AGG - chr8_3:44239155-44239174 Msa1289790:intron 45.0%
GAGGTGAAGGAGTTGTTCAA+AGG - chr8_3:44239168-44239187 Msa1289790:intron 45.0%
TGAGGAGATTGTGCATTGAG+AGG + chr8_3:44239327-44239346 None:intergenic 45.0%
CAATCTCCTCAGATGATGCT+TGG - chr8_3:44239336-44239355 Msa1289790:CDS 45.0%
TCTCCTCAGATGATGCTTGG+TGG - chr8_3:44239339-44239358 Msa1289790:CDS 50.0%
TCTCCACCAAGCATCATCTG+AGG + chr8_3:44239345-44239364 None:intergenic 50.0%
GCGTGTTCAGGTCCACCAAA+TGG + chr8_3:44239396-44239415 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 44238725 44239461 44238725 ID=Msa1289790;Name=Msa1289790
chr8_3 mRNA 44238725 44239461 44238725 ID=Msa1289790-mRNA-1;Parent=Msa1289790;Name=Msa1289790-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|153
chr8_3 exon 44239322 44239461 44239322 ID=Msa1289790-mRNA-1:exon:12117;Parent=Msa1289790-mRNA-1
chr8_3 exon 44238725 44239043 44238725 ID=Msa1289790-mRNA-1:exon:12116;Parent=Msa1289790-mRNA-1
chr8_3 CDS 44239322 44239461 44239322 ID=Msa1289790-mRNA-1:cds;Parent=Msa1289790-mRNA-1
chr8_3 CDS 44238725 44239043 44238725 ID=Msa1289790-mRNA-1:cds;Parent=Msa1289790-mRNA-1
Gene Sequence

>Msa1289790

ATGAGTACGAGTCAAGGTGGTAAGAAACTAACCGAAGATGATGCCTTTGCATATCTTAAGGCAGTCAAGGATGTCTTCAGAGAACAAATGGAAAACTATTCTTACTTTTTGAAAATAATGAAGGATTACAAAGATAACAGAATTAATTACCATGATGTTGTAACGGAGGTGAAGGAGTTGTTCAAAGGACATAATGATCTGATTACGGGATTCAACATCTTTGCACCAAGTGGTTATCAAATCCCAATTTCAGAAGAAGATGAACAACCCCTTAGGAATCTTGATGAATGTGAAATGACAATTCCATTGGAAGAATTAAAACCTCTCAATGCACAATCTCCTCAGATGATGCTTGGTGGAGAGCATATCACAACTCCATCAAGGAATGAACCATTTGGTGGACCTGAACACGCTAATGAGTTTGTCAGGAAAGTTAAGGTAATTTTTTATAAACAAATG

Protein sequence

>Msa1289790

MSTSQGGKKLTEDDAFAYLKAVKDVFREQMENYSYFLKIMKDYKDNRINYHDVVTEVKELFKGHNDLITGFNIFAPSGYQIPISEEDEQPLRNLDECEMTIPLEELKPLNAQSPQMMLGGEHITTPSRNEPFGGPEHANEFVRKVKVIFYKQM