AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1291920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291920 MtrunA17_Chr8g0366501 98.670 376 2 2 1 373 1 376 0.0 750
Msa1291920 MtrunA17_Chr4g0063401 37.624 202 89 6 88 282 77 248 1.53e-34 127
Msa1291920 MtrunA17_Chr4g0063361 33.333 201 97 5 79 279 76 239 1.67e-27 108
Msa1291920 MtrunA17_Chr4g0063391 28.405 257 115 5 34 283 16 210 1.13e-25 103
Msa1291920 MtrunA17_Chr3g0098251 28.571 224 105 5 59 279 4 175 1.48e-21 91.7
Msa1291920 MtrunA17_Chr4g0063371 36.975 119 46 2 79 197 56 145 8.82e-17 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291920 AT1G32360.1 60.560 393 121 13 3 373 4 384 3.74e-130 378
Msa1291920 AT3G19360.1 36.424 302 155 10 83 372 98 374 9.16e-46 161
Msa1291920 AT3G19360.2 36.424 302 155 10 83 372 98 374 9.16e-46 161
Msa1291920 AT2G35430.1 41.739 230 63 9 166 373 72 252 1.85e-41 146
Msa1291920 AT2G35430.1 38.012 171 68 7 25 190 31 168 4.38e-20 89.0

Find 136 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGGAGGAGGAGGAGG+TGG 0.204425 8_3:+47549848 Msa1291920:CDS
GGTGGAGGTGGTGGAGGAGG+AGG 0.223597 8_3:+47549842 Msa1291920:CDS
GGAGGTGGTGGAGGAGGAGG+AGG 0.235103 8_3:+47549845 Msa1291920:CDS
TATGAATAATAGGTCTTATA+AGG 0.235365 8_3:+47549688 Msa1291920:CDS
AGTTTAGGTCCTGGTGGTTA+TGG 0.246502 8_3:+47549815 Msa1291920:CDS
GGTGGTGGAGGTGGTGGAGG+AGG 0.265178 8_3:+47549839 Msa1291920:CDS
ACTGAGGAAGGTTGTCCTTA+TGG 0.268283 8_3:+47549734 Msa1291920:CDS
TATGGTGGTGGTGGAGGTGG+TGG 0.279719 8_3:+47549833 Msa1291920:CDS
CACATTATCAGCATGAGTTT+TGG 0.299688 8_3:-47551401 None:intergenic
ATAACTTATATTCCCAATCT+TGG 0.301692 8_3:+47551596 Msa1291920:three_prime_UTR
GGTGGTGGTGGAGGTGGTGG+AGG 0.301864 8_3:+47549836 Msa1291920:CDS
GGTTATGGTGGTGGTGGAGG+TGG 0.308759 8_3:+47549830 Msa1291920:CDS
CAATTCGAAGGCTTCAAATT+AGG 0.308899 8_3:-47549947 None:intergenic
AAATTACAGTTCGTTATATA+AGG 0.316371 8_3:-47549518 None:intergenic
CAGATATGGAGGTGGGCTTA+TGG 0.316746 8_3:+47551318 Msa1291920:CDS
TTGTTCAATTCACAGATAAT+TGG 0.319647 8_3:+47551743 Msa1291920:three_prime_UTR
TTGGATCTAATTTAAGATTC+CGG 0.332594 8_3:+47551898 Msa1291920:three_prime_UTR
GATAAAATCAGTAGGATATA+TGG 0.337131 8_3:+47551541 Msa1291920:CDS
GAAAGTGTGGCGATTAGTTT+AGG 0.346387 8_3:+47549800 Msa1291920:CDS
TTGGGGAGAGTATGAATAAT+AGG 0.346414 8_3:+47549678 Msa1291920:CDS
CTAATTTGAAGCCTTCGAAT+TGG 0.351114 8_3:+47549948 Msa1291920:CDS
GGTTCAGATGTTTATCATCT+TGG 0.353688 8_3:+47551445 Msa1291920:CDS
TTCTGATGAGCTCTCTGTCC+TGG 0.376334 8_3:-47551505 None:intergenic
CAGCTGGTGCGGCCGCGGTT+GGG 0.377275 8_3:+47549918 Msa1291920:CDS
GCAGCTGGTGCGGCCGCGGT+TGG 0.388240 8_3:+47549917 Msa1291920:CDS
TACGTCGGCCGCCGGAGAAT+TGG 0.393860 8_3:+47549561 Msa1291920:CDS
CTGTAGCACCATGAGCAAAA+TGG 0.399102 8_3:-47550030 None:intergenic
GTTGCATCAAATCCTCCTAT+TGG 0.403665 8_3:+47551424 Msa1291920:CDS
TGTAATAAGTGGGAGATGAC+TGG 0.409320 8_3:+47549983 Msa1291920:CDS
GGTGGTTATGGTGGTGGTGG+AGG 0.415973 8_3:+47549827 Msa1291920:CDS
GGTGCGGCCGCGGTTGGGAA+TGG 0.416021 8_3:+47549923 Msa1291920:CDS
GGTGGTGGTGGCAGTGGAGG+AGG 0.418521 8_3:+47549419 Msa1291920:CDS
ATGACTGGGTATTGTCCCTT+TGG 0.419828 8_3:+47549998 Msa1291920:CDS
ATGAATAATAGGTCTTATAA+GGG 0.419939 8_3:+47549689 Msa1291920:CDS
GGAGGAGGAGGTGGGAGTGT+TGG 0.423488 8_3:+47549857 Msa1291920:CDS
TCGAATCGTGCGAAAGCGAT+TGG 0.423638 8_3:+47549443 Msa1291920:CDS
CGGAAGATGAGTATCGTTAC+TGG 0.423843 8_3:+47549300 Msa1291920:CDS
GGTGTTGTTGTTGTTGCAGC+TGG 0.426984 8_3:+47549902 Msa1291920:CDS
GTTGGTAGTGTTGGTGGTAG+TGG 0.432301 8_3:+47549875 Msa1291920:CDS
CAAGATGGTGGTGGTGGCAG+TGG 0.435000 8_3:+47549413 Msa1291920:CDS
TGATAAACATCTGAACCAAT+AGG 0.439056 8_3:-47551439 None:intergenic
AAACTAGGATTTGTAATAAG+TGG 0.442008 8_3:+47549972 Msa1291920:CDS
ACTGCAGAATTACACAGATA+TGG 0.443856 8_3:+47551304 Msa1291920:intron
GGTGGGAGTGTTGGTAGTGT+TGG 0.443949 8_3:+47549866 Msa1291920:CDS
TAATATAACAAGATGGAAAC+GGG 0.444193 8_3:-47551675 None:intergenic
TTGCTTCTCGATTTCTTACT+CGG 0.445185 8_3:-47549386 None:intergenic
AGCCTTCGAATTGGAAAACT+AGG 0.448493 8_3:+47549957 Msa1291920:CDS
TGTTGCGTCTGACACAAAAC+AGG 0.449665 8_3:+47551369 Msa1291920:CDS
GGAGTGTGATTGAGCAGCCT+AGG 0.450759 8_3:+47549615 Msa1291920:CDS
GATGGTGGTGGTGGCAGTGG+AGG 0.455113 8_3:+47549416 Msa1291920:CDS
GGTCAACCGACCAAGCTGAC+TGG 0.458039 8_3:+47549273 Msa1291920:CDS
GAACAAAGTGCTACAGGTGC+TGG 0.461349 8_3:-47551726 None:intergenic
GGGAGTGTTGGTAGTGTTGG+TGG 0.465014 8_3:+47549869 Msa1291920:CDS
CGAATCGTGCGAAAGCGATT+GGG 0.465049 8_3:+47549444 Msa1291920:CDS
GTGGTGGAGGAGGAGGAGGT+GGG 0.465072 8_3:+47549849 Msa1291920:CDS
ATAATATAACAAGATGGAAA+CGG 0.480245 8_3:-47551676 None:intergenic
CCTGGTGGTTATGGTGGTGG+TGG 0.482325 8_3:+47549824 Msa1291920:CDS
AGTTTCAGATACCGACGGTT+GGG 0.486443 8_3:+47549642 Msa1291920:CDS
GGTCCTGGTGGTTATGGTGG+TGG 0.487385 8_3:+47549821 Msa1291920:CDS
CTTCCGTCGCCCAGTCAGCT+TGG 0.490823 8_3:-47549283 None:intergenic
TGTTGCAGCTGGTGCGGCCG+CGG 0.493113 8_3:+47549913 Msa1291920:CDS
AAGATGGAAACGGGAAATCA+TGG 0.493467 8_3:-47551666 None:intergenic
AACTAGGATTTGTAATAAGT+GGG 0.496877 8_3:+47549973 Msa1291920:CDS
AGTGTTGGTGGTAGTGGTAG+TGG 0.513157 8_3:+47549881 Msa1291920:CDS
CAATGGAAGGTCTCTGTGAA+GGG 0.514650 8_3:-47551471 None:intergenic
CGTCGCCCAGTCAGCTTGGT+CGG 0.519038 8_3:-47549279 None:intergenic
AAAGTACAACTATCTCCATA+AGG 0.520248 8_3:-47549749 None:intergenic
TCAGTAGGATATATGGCGAT+TGG 0.521816 8_3:+47551548 Msa1291920:CDS
GTCAACCGACCAAGCTGACT+GGG 0.522000 8_3:+47549274 Msa1291920:CDS
GTGGCGATTAGTTTAGGTCC+TGG 0.524638 8_3:+47549806 Msa1291920:CDS
ACAATCTCCTGCCAATTCTC+CGG 0.526020 8_3:-47549572 None:intergenic
CCGGCGGAGCCGCCGATAGT+TGG 0.526889 8_3:+47549252 Msa1291920:CDS
GGAGAATTGGCAGGAGATTG+TGG 0.530968 8_3:+47549574 Msa1291920:CDS
GCTACAGGTGCTGGAAGCTA+AGG 0.534984 8_3:-47551717 None:intergenic
CCGTCTTCAAATTCACGTGC+AGG 0.538664 8_3:+47549353 Msa1291920:CDS
AAAATGGCATTTGTTACCAA+AGG 0.546912 8_3:-47550014 None:intergenic
GACAGAGAGCTCATCAGAAG+TGG 0.547469 8_3:+47551509 Msa1291920:CDS
TGAATTGAACAAAGTGCTAC+AGG 0.547737 8_3:-47551732 None:intergenic
TCGGCCGCCGGAGAATTGGC+AGG 0.548725 8_3:+47549565 Msa1291920:CDS
ACTATCGGCGGCTCCGCCGG+TGG 0.550887 8_3:-47549249 None:intergenic
GGTATCTGAAACTCCTCCCT+AGG 0.552310 8_3:-47549632 None:intergenic
GGCCTCTGACTCACAATGGA+AGG 0.553370 8_3:-47551484 None:intergenic
TCGAGAAGCAATTCGCAAGA+TGG 0.554276 8_3:+47549398 Msa1291920:CDS
TTAGGTCCTGGTGGTTATGG+TGG 0.556983 8_3:+47549818 Msa1291920:CDS
GAGTGTGATTGAGCAGCCTA+GGG 0.557000 8_3:+47549616 Msa1291920:CDS
AAATGGCATTTGTTACCAAA+GGG 0.557541 8_3:-47550013 None:intergenic
GTAATAAGTGGGAGATGACT+GGG 0.557999 8_3:+47549984 Msa1291920:CDS
AATTCGCAAGATGGTGGTGG+TGG 0.565492 8_3:+47549407 Msa1291920:CDS
TCCATTGTGAGTCAGAGGCC+AGG 0.568730 8_3:+47551487 Msa1291920:CDS
ACAATGGAAGGTCTCTGTGA+AGG 0.570880 8_3:-47551472 None:intergenic
TTATGCTGCAAGTTTCGTGT+GGG 0.573709 8_3:+47549488 Msa1291920:CDS
GGAGGAGCTACGTCGGCCGC+CGG 0.573942 8_3:+47549553 Msa1291920:CDS
TACTGGAACGGAAACGACGC+CGG 0.576258 8_3:+47549317 Msa1291920:CDS
GACCTTCCATTGTGAGTCAG+AGG 0.582385 8_3:+47551482 Msa1291920:CDS
TATGGAGGTGGGCTTATGGA+AGG 0.583879 8_3:+47551322 Msa1291920:CDS
TATTCGATAATATAACAAGA+TGG 0.584070 8_3:-47551682 None:intergenic
ATTATGCTGCAAGTTTCGTG+TGG 0.588794 8_3:+47549487 Msa1291920:CDS
ATTCACAGATAATTGGTCTG+TGG 0.589907 8_3:+47551750 Msa1291920:three_prime_UTR
AGCAATTCGCAAGATGGTGG+TGG 0.591086 8_3:+47549404 Msa1291920:CDS
GAATTACACAGATATGGAGG+TGG 0.593880 8_3:+47551310 Msa1291920:intron
TCCTGGCCTCTGACTCACAA+TGG 0.594508 8_3:-47551488 None:intergenic
TGTTGTTGTTGCAGCTGGTG+CGG 0.594830 8_3:+47549907 Msa1291920:CDS
CGACCAAGCTGACTGGGCGA+CGG 0.595148 8_3:+47549280 Msa1291920:CDS
GATGAGTATCGTTACTGGAA+CGG 0.598603 8_3:+47549305 Msa1291920:CDS
CAAAACTCATGCTGATAATG+TGG 0.598802 8_3:+47551402 Msa1291920:CDS
GTCGTTCAAATCATCACCAC+CGG 0.600321 8_3:+47549233 Msa1291920:CDS
CCACCACCACCATAACCACC+AGG 0.606081 8_3:-47549824 None:intergenic
AGAGCTCATCAGAAGTGGAA+AGG 0.606297 8_3:+47551514 Msa1291920:CDS
GAGTTTCAGATACCGACGGT+TGG 0.612303 8_3:+47549641 Msa1291920:CDS
AAAGTACTCGACCCAACCGT+CGG 0.614579 8_3:-47549653 None:intergenic
CCTGCACGTGAATTTGAAGA+CGG 0.617302 8_3:-47549353 None:intergenic
ATCGAAGAATCGTGAAAGTG+TGG 0.618402 8_3:+47549787 Msa1291920:CDS
AAGGTCCCGATAAAATCAGT+AGG 0.622761 8_3:+47551533 Msa1291920:CDS
GGAGGAGTTTCAGATACCGA+CGG 0.623970 8_3:+47549637 Msa1291920:CDS
TGTGATTGAGCAGCCTAGGG+AGG 0.629299 8_3:+47549619 Msa1291920:CDS
TTGGTCGGTTGACCAACTAT+CGG 0.633102 8_3:-47549264 None:intergenic
AATTACACAGATATGGAGGT+GGG 0.635355 8_3:+47551311 Msa1291920:CDS
TAAACATCTGAACCAATAGG+AGG 0.638482 8_3:-47551436 None:intergenic
AGAAGCAATTCGCAAGATGG+TGG 0.638549 8_3:+47549401 Msa1291920:CDS
TGAATAATAGGTCTTATAAG+GGG 0.644881 8_3:+47549690 Msa1291920:CDS
TGGCTGCTCATGAAGAAGAG+AGG 0.650335 8_3:+47549594 Msa1291920:CDS
ACAATGGATTACAACAGAGA+CGG 0.651622 8_3:+47549206 None:intergenic
ATGGAAGGAGAAAGCAGAGA+CGG 0.651866 8_3:+47551337 Msa1291920:CDS
ATCTCCTGCCAATTCTCCGG+CGG 0.654680 8_3:-47549569 None:intergenic
ATCTTAAATTAGATCCAACA+TGG 0.654694 8_3:-47551893 None:intergenic
GCGATTAGTTTAGGTCCTGG+TGG 0.657986 8_3:+47549809 Msa1291920:CDS
TATGCTGCAAGTTTCGTGTG+GGG 0.660211 8_3:+47549489 Msa1291920:CDS
TTGGTCTGTGGATTTGTCTG+CGG 0.660714 8_3:+47551762 Msa1291920:three_prime_UTR
TGGAACGGAAACGACGCCGG+CGG 0.668874 8_3:+47549320 Msa1291920:CDS
GCAGAATTACACAGATATGG+AGG 0.676863 8_3:+47551307 Msa1291920:intron
GTTCAAATCATCACCACCGG+CGG 0.687955 8_3:+47549236 Msa1291920:CDS
ATTAGGTCCATTCCCAACCG+CGG 0.701157 8_3:-47549930 None:intergenic
CTTCTCGATTTCTTACTCGG+CGG 0.702870 8_3:-47549383 None:intergenic
ATAGTGTGGAGGAGCTACGT+CGG 0.704848 8_3:+47549546 Msa1291920:CDS
GTCGGTTGACCAACTATCGG+CGG 0.708168 8_3:-47549261 None:intergenic
GTTGCGTCTGACACAAAACA+GGG 0.716294 8_3:+47551370 Msa1291920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTAAAGATTTTAATTTTA+TGG + chr8_3:47550094-47550113 Msa1291920:intron 10.0%
!! ATTTATATAAAATAGGTTAT+AGG - chr8_3:47551261-47551280 None:intergenic 10.0%
!!! TTTTAATTTTATGGTAATTG+AGG + chr8_3:47550103-47550122 Msa1291920:intron 15.0%
!! AAGACAGATTTATATAAAAT+AGG - chr8_3:47551268-47551287 None:intergenic 15.0%
!! AAATTACAGTTCGTTATATA+AGG - chr8_3:47549521-47549540 None:intergenic 20.0%
!!! TATGAATAATAGGTCTTATA+AGG + chr8_3:47549688-47549707 Msa1291920:CDS 20.0%
!!! ATGAATAATAGGTCTTATAA+GGG + chr8_3:47549689-47549708 Msa1291920:CDS 20.0%
!!! TTGTAAGAAATTTTATACTG+AGG + chr8_3:47549718-47549737 Msa1291920:CDS 20.0%
!! ACATAACAAAATCATCAAAA+TGG - chr8_3:47550068-47550087 None:intergenic 20.0%
!!! GGTTTTTTCTTCATTTAATA+TGG + chr8_3:47550275-47550294 Msa1291920:intron 20.0%
!!! ATATATCCTACTGATTTTAT+CGG - chr8_3:47551542-47551561 None:intergenic 20.0%
!! ATAATATAACAAGATGGAAA+CGG - chr8_3:47551679-47551698 None:intergenic 20.0%
!! TATTCGATAATATAACAAGA+TGG - chr8_3:47551685-47551704 None:intergenic 20.0%
!! TGAATAATAGGTCTTATAAG+GGG + chr8_3:47549690-47549709 Msa1291920:CDS 25.0%
!! AAGAAATTTTATACTGAGGA+AGG + chr8_3:47549722-47549741 Msa1291920:CDS 25.0%
! AAACTAGGATTTGTAATAAG+TGG + chr8_3:47549972-47549991 Msa1291920:CDS 25.0%
! AACTAGGATTTGTAATAAGT+GGG + chr8_3:47549973-47549992 Msa1291920:CDS 25.0%
!!! GATGATTTTGTTATGTGAAT+TGG + chr8_3:47550072-47550091 Msa1291920:intron 25.0%
!!! ATAGAAAGTGCTATTAGAAT+TGG + chr8_3:47550149-47550168 Msa1291920:intron 25.0%
!! GTTATAAATAGTTTGTGTTG+TGG + chr8_3:47550175-47550194 Msa1291920:intron 25.0%
!! TTACCTTTTGGATACAATTT+TGG + chr8_3:47550216-47550235 Msa1291920:intron 25.0%
!! TACCTTTTGGATACAATTTT+GGG + chr8_3:47550217-47550236 Msa1291920:intron 25.0%
!! TCTTTTTATCCATACAAGAT+TGG + chr8_3:47550254-47550273 Msa1291920:intron 25.0%
! AAGAAAAAACCAATCTTGTA+TGG - chr8_3:47550266-47550285 None:intergenic 25.0%
!! TTTTCATTGTGCAAAACATA+AGG - chr8_3:47550546-47550565 None:intergenic 25.0%
! ATGAAAAGTAATCGCAAAAA+AGG - chr8_3:47550654-47550673 None:intergenic 25.0%
!! ATTTGAATTTGAAGCATACA+AGG - chr8_3:47550680-47550699 None:intergenic 25.0%
!! TTTTCTTAATCAGAAAGTGA+AGG - chr8_3:47550710-47550729 None:intergenic 25.0%
!!! TATATCCTACTGATTTTATC+GGG - chr8_3:47551541-47551560 None:intergenic 25.0%
! GATAAAATCAGTAGGATATA+TGG + chr8_3:47551541-47551560 Msa1291920:CDS 25.0%
! ATAACTTATATTCCCAATCT+TGG + chr8_3:47551596-47551615 Msa1291920:three_prime_UTR 25.0%
!! TTTTTCATGTTTCCAAGATT+GGG - chr8_3:47551611-47551630 None:intergenic 25.0%
!! ATTTTTCATGTTTCCAAGAT+TGG - chr8_3:47551612-47551631 None:intergenic 25.0%
! AATCTTGGAAACATGAAAAA+TGG + chr8_3:47551611-47551630 Msa1291920:three_prime_UTR 25.0%
! TAATATAACAAGATGGAAAC+GGG - chr8_3:47551678-47551697 None:intergenic 25.0%
! TTGTTCAATTCACAGATAAT+TGG + chr8_3:47551743-47551762 Msa1291920:three_prime_UTR 25.0%
! ATCTTAAATTAGATCCAACA+TGG - chr8_3:47551896-47551915 None:intergenic 25.0%
!! TTGGATCTAATTTAAGATTC+CGG + chr8_3:47551898-47551917 Msa1291920:three_prime_UTR 25.0%
AAAGTACAACTATCTCCATA+AGG - chr8_3:47549752-47549771 None:intergenic 30.0%
! AAATGGCATTTGTTACCAAA+GGG - chr8_3:47550016-47550035 None:intergenic 30.0%
! AAAATGGCATTTGTTACCAA+AGG - chr8_3:47550017-47550036 None:intergenic 30.0%
TTCCCAAAATTGTATCCAAA+AGG - chr8_3:47550222-47550241 None:intergenic 30.0%
CCAAAACAAAATTGACACTA+GGG - chr8_3:47550354-47550373 None:intergenic 30.0%
ACCAAAACAAAATTGACACT+AGG - chr8_3:47550355-47550374 None:intergenic 30.0%
!!! TTTTGTTTTGGTTGATGCTA+AGG + chr8_3:47550363-47550382 Msa1291920:intron 30.0%
TGATATGTAGTATGTGAATG+TGG + chr8_3:47550401-47550420 Msa1291920:intron 30.0%
ATTGCATAGATTACTCAAGT+CGG - chr8_3:47550462-47550481 None:intergenic 30.0%
!!! GCAAATTTCCAAGTGTTTTT+TGG + chr8_3:47550616-47550635 Msa1291920:intron 30.0%
ACTGTAAACCAAAAAACACT+TGG - chr8_3:47550627-47550646 None:intergenic 30.0%
GAAAAGAATAGTTTCATGAG+TGG + chr8_3:47550750-47550769 Msa1291920:intron 30.0%
TAATCTGTGTTGTCAAATAG+TGG + chr8_3:47550806-47550825 Msa1291920:intron 30.0%
TGTACTTAATCGCATATTTG+CGG - chr8_3:47550879-47550898 None:intergenic 30.0%
ACTTTCATTGCTTTGATGTT+TGG + chr8_3:47550999-47551018 Msa1291920:intron 30.0%
GTAATGTATATGTGCATGTT+TGG - chr8_3:47551166-47551185 None:intergenic 30.0%
TGATAAACATCTGAACCAAT+AGG - chr8_3:47551442-47551461 None:intergenic 30.0%
! TTGTTCTTTTGTTACCATGT+TGG + chr8_3:47551879-47551898 Msa1291920:three_prime_UTR 30.0%
TTGCTTCTCGATTTCTTACT+CGG - chr8_3:47549389-47549408 None:intergenic 35.0%
! CTGTAATTTTGCTCATAGTG+TGG + chr8_3:47549532-47549551 Msa1291920:CDS 35.0%
! TAATTTTGCTCATAGTGTGG+AGG + chr8_3:47549535-47549554 Msa1291920:CDS 35.0%
! TTGGGGAGAGTATGAATAAT+AGG + chr8_3:47549678-47549697 Msa1291920:CDS 35.0%
CAATTCGAAGGCTTCAAATT+AGG - chr8_3:47549950-47549969 None:intergenic 35.0%
! CTAATTTGAAGCCTTCGAAT+TGG + chr8_3:47549948-47549967 Msa1291920:CDS 35.0%
!! ATCCTAGTTTTCCAATTCGA+AGG - chr8_3:47549962-47549981 None:intergenic 35.0%
!!! AACAAATGCCATTTTGCTCA+TGG + chr8_3:47550022-47550041 Msa1291920:CDS 35.0%
!! CCCTAGTGTCAATTTTGTTT+TGG + chr8_3:47550351-47550370 Msa1291920:intron 35.0%
CATTGTGCAAAACATAAGGA+AGG - chr8_3:47550542-47550561 None:intergenic 35.0%
AAGAATAGTTTCATGAGTGG+TGG + chr8_3:47550753-47550772 Msa1291920:intron 35.0%
AGAATAGTTTCATGAGTGGT+GGG + chr8_3:47550754-47550773 Msa1291920:intron 35.0%
TGTTGTCAAATAGCGTTTAC+AGG + chr8_3:47550902-47550921 Msa1291920:intron 35.0%
GTTGTCAAATAGCGTTTACA+GGG + chr8_3:47550903-47550922 Msa1291920:intron 35.0%
! TGAAAGTATGCTGTCAATTG+CGG - chr8_3:47550986-47551005 None:intergenic 35.0%
AAACATGCACGTAATGAACA+AGG - chr8_3:47551025-47551044 None:intergenic 35.0%
TTGTTCATTACGTGCATGTT+TGG + chr8_3:47551024-47551043 Msa1291920:intron 35.0%
ACTGCAGAATTACACAGATA+TGG + chr8_3:47551304-47551323 Msa1291920:intron 35.0%
AATTACACAGATATGGAGGT+GGG + chr8_3:47551311-47551330 Msa1291920:CDS 35.0%
!!! TTATCAGCATGAGTTTTGGA+AGG - chr8_3:47551400-47551419 None:intergenic 35.0%
! CACATTATCAGCATGAGTTT+TGG - chr8_3:47551404-47551423 None:intergenic 35.0%
! CAAAACTCATGCTGATAATG+TGG + chr8_3:47551402-47551421 Msa1291920:CDS 35.0%
TAAACATCTGAACCAATAGG+AGG - chr8_3:47551439-47551458 None:intergenic 35.0%
GGTTCAGATGTTTATCATCT+TGG + chr8_3:47551445-47551464 Msa1291920:CDS 35.0%
TTGGAAACATGAAAAATGGC+TGG + chr8_3:47551615-47551634 Msa1291920:three_prime_UTR 35.0%
! TGAATTGAACAAAGTGCTAC+AGG - chr8_3:47551735-47551754 None:intergenic 35.0%
! ATTCACAGATAATTGGTCTG+TGG + chr8_3:47551750-47551769 Msa1291920:three_prime_UTR 35.0%
!!! CTGAGTTTTGAACGTAACAT+CGG + chr8_3:47551819-47551838 Msa1291920:three_prime_UTR 35.0%
! GATGAGTATCGTTACTGGAA+CGG + chr8_3:47549305-47549324 Msa1291920:CDS 40.0%
ATTATGCTGCAAGTTTCGTG+TGG + chr8_3:47549487-47549506 Msa1291920:CDS 40.0%
TTATGCTGCAAGTTTCGTGT+GGG + chr8_3:47549488-47549507 Msa1291920:CDS 40.0%
! TTGGGTCGAGTACTTTTGTT+GGG + chr8_3:47549660-47549679 Msa1291920:CDS 40.0%
ATCGAAGAATCGTGAAAGTG+TGG + chr8_3:47549787-47549806 Msa1291920:CDS 40.0%
!! GAAAGTGTGGCGATTAGTTT+AGG + chr8_3:47549800-47549819 Msa1291920:CDS 40.0%
AGCCTTCGAATTGGAAAACT+AGG + chr8_3:47549957-47549976 Msa1291920:CDS 40.0%
TGTAATAAGTGGGAGATGAC+TGG + chr8_3:47549983-47550002 Msa1291920:CDS 40.0%
GTAATAAGTGGGAGATGACT+GGG + chr8_3:47549984-47550003 Msa1291920:CDS 40.0%
! TAGTTTGTGTTGTGGAGTTG+TGG + chr8_3:47550183-47550202 Msa1291920:intron 40.0%
! GGCAGTGTTTTCTTACCTTT+TGG + chr8_3:47550204-47550223 Msa1291920:intron 40.0%
GTGGGTTCAGAATGAATAGA+TGG + chr8_3:47550772-47550791 Msa1291920:intron 40.0%
TATGATAGCACAGTACGTAG+TGG + chr8_3:47550930-47550949 Msa1291920:intron 40.0%
TACGTGCATGTTTGGATTGA+CGG + chr8_3:47551032-47551051 Msa1291920:intron 40.0%
TATGTGCATGTTTGGATCAG+CGG - chr8_3:47551158-47551177 None:intergenic 40.0%
GCAGAATTACACAGATATGG+AGG + chr8_3:47551307-47551326 Msa1291920:intron 40.0%
GAATTACACAGATATGGAGG+TGG + chr8_3:47551310-47551329 Msa1291920:intron 40.0%
GTTGCATCAAATCCTCCTAT+TGG + chr8_3:47551424-47551443 Msa1291920:CDS 40.0%
AAGGTCCCGATAAAATCAGT+AGG + chr8_3:47551533-47551552 Msa1291920:CDS 40.0%
TCAGTAGGATATATGGCGAT+TGG + chr8_3:47551548-47551567 Msa1291920:CDS 40.0%
AAGATGGAAACGGGAAATCA+TGG - chr8_3:47551669-47551688 None:intergenic 40.0%
GTCGTTCAAATCATCACCAC+CGG + chr8_3:47549233-47549252 Msa1291920:CDS 45.0%
TTGGTCGGTTGACCAACTAT+CGG - chr8_3:47549267-47549286 None:intergenic 45.0%
! CGGAAGATGAGTATCGTTAC+TGG + chr8_3:47549300-47549319 Msa1291920:CDS 45.0%
CCTGCACGTGAATTTGAAGA+CGG - chr8_3:47549356-47549375 None:intergenic 45.0%
CTTCTCGATTTCTTACTCGG+CGG - chr8_3:47549386-47549405 None:intergenic 45.0%
TCGAGAAGCAATTCGCAAGA+TGG + chr8_3:47549398-47549417 Msa1291920:CDS 45.0%
AGAAGCAATTCGCAAGATGG+TGG + chr8_3:47549401-47549420 Msa1291920:CDS 45.0%
TATGCTGCAAGTTTCGTGTG+GGG + chr8_3:47549489-47549508 Msa1291920:CDS 45.0%
ACAATCTCCTGCCAATTCTC+CGG - chr8_3:47549575-47549594 None:intergenic 45.0%
AGTTTCAGATACCGACGGTT+GGG + chr8_3:47549642-47549661 Msa1291920:CDS 45.0%
! GTTGGGTCGAGTACTTTTGT+TGG + chr8_3:47549659-47549678 Msa1291920:CDS 45.0%
! TGGGTCGAGTACTTTTGTTG+GGG + chr8_3:47549661-47549680 Msa1291920:CDS 45.0%
ACTGAGGAAGGTTGTCCTTA+TGG + chr8_3:47549734-47549753 Msa1291920:CDS 45.0%
AGTTTAGGTCCTGGTGGTTA+TGG + chr8_3:47549815-47549834 Msa1291920:CDS 45.0%
! ATGACTGGGTATTGTCCCTT+TGG + chr8_3:47549998-47550017 Msa1291920:CDS 45.0%
CTGTAGCACCATGAGCAAAA+TGG - chr8_3:47550033-47550052 None:intergenic 45.0%
!!! CATTTTGCTCATGGTGCTAC+AGG + chr8_3:47550031-47550050 Msa1291920:CDS 45.0%
TTGACACTAGGGACAATGCA+GGG - chr8_3:47550343-47550362 None:intergenic 45.0%
ATTGACACTAGGGACAATGC+AGG - chr8_3:47550344-47550363 None:intergenic 45.0%
CGGTGAGATTGGCATAATCA+TGG - chr8_3:47551138-47551157 None:intergenic 45.0%
ATGGAAGGAGAAAGCAGAGA+CGG + chr8_3:47551337-47551356 Msa1291920:CDS 45.0%
TGTTGCGTCTGACACAAAAC+AGG + chr8_3:47551369-47551388 Msa1291920:CDS 45.0%
GTTGCGTCTGACACAAAACA+GGG + chr8_3:47551370-47551389 Msa1291920:CDS 45.0%
CAATGGAAGGTCTCTGTGAA+GGG - chr8_3:47551474-47551493 None:intergenic 45.0%
ACAATGGAAGGTCTCTGTGA+AGG - chr8_3:47551475-47551494 None:intergenic 45.0%
AGAGCTCATCAGAAGTGGAA+AGG + chr8_3:47551514-47551533 Msa1291920:CDS 45.0%
! TCATGGCGTTTTCAGCTACA+TGG - chr8_3:47551652-47551671 None:intergenic 45.0%
TTGGTCTGTGGATTTGTCTG+CGG + chr8_3:47551762-47551781 Msa1291920:three_prime_UTR 45.0%
GTTCAAATCATCACCACCGG+CGG + chr8_3:47549236-47549255 Msa1291920:CDS 50.0%
CCGTCTTCAAATTCACGTGC+AGG + chr8_3:47549353-47549372 Msa1291920:CDS 50.0%
! AGCAATTCGCAAGATGGTGG+TGG + chr8_3:47549404-47549423 Msa1291920:CDS 50.0%
!! AATTCGCAAGATGGTGGTGG+TGG + chr8_3:47549407-47549426 Msa1291920:CDS 50.0%
TCGAATCGTGCGAAAGCGAT+TGG + chr8_3:47549443-47549462 Msa1291920:CDS 50.0%
CGAATCGTGCGAAAGCGATT+GGG + chr8_3:47549444-47549463 Msa1291920:CDS 50.0%
ATAGTGTGGAGGAGCTACGT+CGG + chr8_3:47549546-47549565 Msa1291920:CDS 50.0%
GGAGAATTGGCAGGAGATTG+TGG + chr8_3:47549574-47549593 Msa1291920:CDS 50.0%
TGGCTGCTCATGAAGAAGAG+AGG + chr8_3:47549594-47549613 Msa1291920:CDS 50.0%
GAGTGTGATTGAGCAGCCTA+GGG + chr8_3:47549616-47549635 Msa1291920:CDS 50.0%
! GGTATCTGAAACTCCTCCCT+AGG - chr8_3:47549635-47549654 None:intergenic 50.0%
GGAGGAGTTTCAGATACCGA+CGG + chr8_3:47549637-47549656 Msa1291920:CDS 50.0%
GAGTTTCAGATACCGACGGT+TGG + chr8_3:47549641-47549660 Msa1291920:CDS 50.0%
! AAAGTACTCGACCCAACCGT+CGG - chr8_3:47549656-47549675 None:intergenic 50.0%
!! GTGGCGATTAGTTTAGGTCC+TGG + chr8_3:47549806-47549825 Msa1291920:CDS 50.0%
!! GCGATTAGTTTAGGTCCTGG+TGG + chr8_3:47549809-47549828 Msa1291920:CDS 50.0%
TTAGGTCCTGGTGGTTATGG+TGG + chr8_3:47549818-47549837 Msa1291920:CDS 50.0%
!! GTTGGTAGTGTTGGTGGTAG+TGG + chr8_3:47549875-47549894 Msa1291920:CDS 50.0%
!! AGTGTTGGTGGTAGTGGTAG+TGG + chr8_3:47549881-47549900 Msa1291920:CDS 50.0%
!! GGTGTTGTTGTTGTTGCAGC+TGG + chr8_3:47549902-47549921 Msa1291920:CDS 50.0%
! TGTTGTTGTTGCAGCTGGTG+CGG + chr8_3:47549907-47549926 Msa1291920:CDS 50.0%
ATTAGGTCCATTCCCAACCG+CGG - chr8_3:47549933-47549952 None:intergenic 50.0%
GTTTGGATCAGCGGTGAGAT+TGG - chr8_3:47551149-47551168 None:intergenic 50.0%
! CAGATATGGAGGTGGGCTTA+TGG + chr8_3:47551318-47551337 Msa1291920:CDS 50.0%
! TATGGAGGTGGGCTTATGGA+AGG + chr8_3:47551322-47551341 Msa1291920:CDS 50.0%
GACCTTCCATTGTGAGTCAG+AGG + chr8_3:47551482-47551501 Msa1291920:CDS 50.0%
TTCTGATGAGCTCTCTGTCC+TGG - chr8_3:47551508-47551527 None:intergenic 50.0%
GACAGAGAGCTCATCAGAAG+TGG + chr8_3:47551509-47551528 Msa1291920:CDS 50.0%
! GAACAAAGTGCTACAGGTGC+TGG - chr8_3:47551729-47551748 None:intergenic 50.0%
GTCGGTTGACCAACTATCGG+CGG - chr8_3:47549264-47549283 None:intergenic 55.0%
GTCAACCGACCAAGCTGACT+GGG + chr8_3:47549274-47549293 Msa1291920:CDS 55.0%
TACTGGAACGGAAACGACGC+CGG + chr8_3:47549317-47549336 Msa1291920:CDS 55.0%
! AGACGGAGTTGTTTCACCGC+CGG - chr8_3:47549339-47549358 None:intergenic 55.0%
ATCTCCTGCCAATTCTCCGG+CGG - chr8_3:47549572-47549591 None:intergenic 55.0%
GGAGTGTGATTGAGCAGCCT+AGG + chr8_3:47549615-47549634 Msa1291920:CDS 55.0%
TGTGATTGAGCAGCCTAGGG+AGG + chr8_3:47549619-47549638 Msa1291920:CDS 55.0%
!! GGTGGGAGTGTTGGTAGTGT+TGG + chr8_3:47549866-47549885 Msa1291920:CDS 55.0%
!! GGGAGTGTTGGTAGTGTTGG+TGG + chr8_3:47549869-47549888 Msa1291920:CDS 55.0%
CGGCGAGTTTAGCAAGATCG+CGG + chr8_3:47551052-47551071 Msa1291920:intron 55.0%
CGAGTTTAGCAAGATCGCGG+TGG + chr8_3:47551055-47551074 Msa1291920:intron 55.0%
GGCCTCTGACTCACAATGGA+AGG - chr8_3:47551487-47551506 None:intergenic 55.0%
TCCATTGTGAGTCAGAGGCC+AGG + chr8_3:47551487-47551506 Msa1291920:CDS 55.0%
TCCTGGCCTCTGACTCACAA+TGG - chr8_3:47551491-47551510 None:intergenic 55.0%
!! GCTACAGGTGCTGGAAGCTA+AGG - chr8_3:47551720-47551739 None:intergenic 55.0%
GGTCAACCGACCAAGCTGAC+TGG + chr8_3:47549273-47549292 Msa1291920:CDS 60.0%
!! CAAGATGGTGGTGGTGGCAG+TGG + chr8_3:47549413-47549432 Msa1291920:CDS 60.0%
GGTCCTGGTGGTTATGGTGG+TGG + chr8_3:47549821-47549840 Msa1291920:CDS 60.0%
CCACCACCACCATAACCACC+AGG - chr8_3:47549827-47549846 None:intergenic 60.0%
!! CCTGGTGGTTATGGTGGTGG+TGG + chr8_3:47549824-47549843 Msa1291920:CDS 60.0%
!! GGTGGTTATGGTGGTGGTGG+AGG + chr8_3:47549827-47549846 Msa1291920:CDS 60.0%
!! GGTTATGGTGGTGGTGGAGG+TGG + chr8_3:47549830-47549849 Msa1291920:CDS 60.0%
!! TATGGTGGTGGTGGAGGTGG+TGG + chr8_3:47549833-47549852 Msa1291920:CDS 60.0%
! GGTGCTACAGCGCTATCGTG+TGG + chr8_3:47550827-47550846 Msa1291920:intron 60.0%
CGTCGCCCAGTCAGCTTGGT+CGG - chr8_3:47549282-47549301 None:intergenic 65.0%
CGACCAAGCTGACTGGGCGA+CGG + chr8_3:47549280-47549299 Msa1291920:CDS 65.0%
CTTCCGTCGCCCAGTCAGCT+TGG - chr8_3:47549286-47549305 None:intergenic 65.0%
TGGAACGGAAACGACGCCGG+CGG + chr8_3:47549320-47549339 Msa1291920:CDS 65.0%
!! GATGGTGGTGGTGGCAGTGG+AGG + chr8_3:47549416-47549435 Msa1291920:CDS 65.0%
TACGTCGGCCGCCGGAGAAT+TGG + chr8_3:47549561-47549580 Msa1291920:CDS 65.0%
! GTGGTGGAGGAGGAGGAGGT+GGG + chr8_3:47549849-47549868 Msa1291920:CDS 65.0%
! GGAGGAGGAGGTGGGAGTGT+TGG + chr8_3:47549857-47549876 Msa1291920:CDS 65.0%
CCAACTATCGGCGGCTCCGC+CGG - chr8_3:47549255-47549274 None:intergenic 70.0%
!! GGTGGTGGTGGCAGTGGAGG+AGG + chr8_3:47549419-47549438 Msa1291920:CDS 70.0%
TCGGCCGCCGGAGAATTGGC+AGG + chr8_3:47549565-47549584 Msa1291920:CDS 70.0%
!! GGTGGTGGTGGAGGTGGTGG+AGG + chr8_3:47549836-47549855 Msa1291920:CDS 70.0%
!! GGTGGTGGAGGTGGTGGAGG+AGG + chr8_3:47549839-47549858 Msa1291920:CDS 70.0%
!! GGTGGAGGTGGTGGAGGAGG+AGG + chr8_3:47549842-47549861 Msa1291920:CDS 70.0%
!! GGAGGTGGTGGAGGAGGAGG+AGG + chr8_3:47549845-47549864 Msa1291920:CDS 70.0%
!! GGTGGTGGAGGAGGAGGAGG+TGG + chr8_3:47549848-47549867 Msa1291920:CDS 70.0%
TGTTGCAGCTGGTGCGGCCG+CGG + chr8_3:47549913-47549932 Msa1291920:CDS 70.0%
ACTATCGGCGGCTCCGCCGG+TGG - chr8_3:47549252-47549271 None:intergenic 75.0%
CCGGCGGAGCCGCCGATAGT+TGG + chr8_3:47549252-47549271 Msa1291920:CDS 75.0%
GGAGGAGCTACGTCGGCCGC+CGG + chr8_3:47549553-47549572 Msa1291920:CDS 75.0%
CAGCTGGTGCGGCCGCGGTT+GGG + chr8_3:47549918-47549937 Msa1291920:CDS 75.0%
GGTGCGGCCGCGGTTGGGAA+TGG + chr8_3:47549923-47549942 Msa1291920:CDS 75.0%
! GCAGCTGGTGCGGCCGCGGT+TGG + chr8_3:47549917-47549936 Msa1291920:CDS 80.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 47549209 47551931 47549209 ID=Msa1291920;Name=Msa1291920
chr8_3 mRNA 47549209 47551931 47549209 ID=Msa1291920-mRNA-1;Parent=Msa1291920;Name=Msa1291920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|343|373
chr8_3 exon 47549209 47550052 47549209 ID=Msa1291920-mRNA-1:exon:13232;Parent=Msa1291920-mRNA-1
chr8_3 exon 47551311 47551931 47551311 ID=Msa1291920-mRNA-1:exon:13233;Parent=Msa1291920-mRNA-1
chr8_3 CDS 47549209 47550052 47549209 ID=Msa1291920-mRNA-1:cds;Parent=Msa1291920-mRNA-1
chr8_3 CDS 47551311 47551588 47551311 ID=Msa1291920-mRNA-1:cds;Parent=Msa1291920-mRNA-1
chr8_3 three_prime_UTR 47551589 47551931 47551589 ID=Msa1291920-mRNA-1:three_prime_utr;Parent=Msa1291920-mRNA-1
Gene Sequence

>Msa1291920

ATGGATTACAACAGAGACGGCAACGTCGTTCAAATCATCACCACCGGCGGAGCCGCCGATAGTTGGTCAACCGACCAAGCTGACTGGGCGACGGAAGATGAGTATCGTTACTGGAACGGAAACGACGCCGGCGGTGAAACAACTCCGTCTTCAAATTCACGTGCAGGAAGTGAGCCGCCGAGTAAGAAATCGAGAAGCAATTCGCAAGATGGTGGTGGTGGCAGTGGAGGAGGATCGAATCGTGCGAAAGCGATTGGGAAAATGTTTTTTAAGACGAAATTATGCTGCAAGTTTCGTGTGGGGACATGTCCTTATATAACGAACTGTAATTTTGCTCATAGTGTGGAGGAGCTACGTCGGCCGCCGGAGAATTGGCAGGAGATTGTGGCTGCTCATGAAGAAGAGAGGAGTGTGATTGAGCAGCCTAGGGAGGAGTTTCAGATACCGACGGTTGGGTCGAGTACTTTTGTTGGGGAGAGTATGAATAATAGGTCTTATAAGGGGAGACATTGTAAGAAATTTTATACTGAGGAAGGTTGTCCTTATGGAGATAGTTGTACTTTTCTTCATGATGAGCAATCGAAGAATCGTGAAAGTGTGGCGATTAGTTTAGGTCCTGGTGGTTATGGTGGTGGTGGAGGTGGTGGAGGAGGAGGAGGTGGGAGTGTTGGTAGTGTTGGTGGTAGTGGTAGTGGTGTTGTTGTTGTTGCAGCTGGTGCGGCCGCGGTTGGGAATGGACCTAATTTGAAGCCTTCGAATTGGAAAACTAGGATTTGTAATAAGTGGGAGATGACTGGGTATTGTCCCTTTGGTAACAAATGCCATTTTGCTCATGGTGCTACAGAATTACACAGATATGGAGGTGGGCTTATGGAAGGAGAAAGCAGAGACGGTGTTTCTGTTGTTGCGTCTGACACAAAACAGGGAGTTCCTTCCAAAACTCATGCTGATAATGTGGTTGCATCAAATCCTCCTATTGGTTCAGATGTTTATCATCTTGGAGTCCCTTCACAGAGACCTTCCATTGTGAGTCAGAGGCCAGGACAGAGAGCTCATCAGAAGTGGAAAGGTCCCGATAAAATCAGTAGGATATATGGCGATTGGATCGATGACTTCGAATAG
Protein sequence

>Msa1291920

MDYNRDGNVVQIITTGGAADSWSTDQADWATEDEYRYWNGNDAGGETTPSSNSRAGSEPPSKKSRSNSQDGGGGSGGGSNRAKAIGKMFFKTKLCCKFRVGTCPYITNCNFAHSVEELRRPPENWQEIVAAHEEERSVIEQPREEFQIPTVGSSTFVGESMNNRSYKGRHCKKFYTEEGCPYGDSCTFLHDEQSKNRESVAISLGPGGYGGGGGGGGGGGGSVGSVGGSGSGVVVVAAGAAAVGNGPNLKPSNWKTRICNKWEMTGYCPFGNKCHFAHGATELHRYGGGLMEGESRDGVSVVASDTKQGVPSKTHADNVVASNPPIGSDVYHLGVPSQRPSIVSQRPGQRAHQKWKGPDKISRIYGDWIDDFE*