AlfalfaGEDB | Msa1294110

M. sativa cultivar zhongmu-4 / Msa1294110

KEGG: K00924|K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009909 GO:0009910 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0060089 GO:0060860 GO:0060862 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:2000026 GO:2000241 GO:2000242
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294110	sp\|P27450\|CST_ARATH	58.270	393	117	3	21	413	34	379	1.89e-163	469

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294110	A0A396GMV8	89.048	420	13	2	14	433	22	408	0.0	751

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294110	MtrunA17_Chr8g0370461	89.048	420	13	2	14	433	22	408	0.0	751
Msa1294110	MtrunA17_Chr8g0370571	79.259	405	27	5	24	427	18	366	0.0	627
Msa1294110	MtrunA17_Chr5g0406841	69.041	365	79	2	62	424	123	455	0.0	514
Msa1294110	MtrunA17_Chr3g0143951	57.979	376	121	3	45	418	42	382	3.78e-148	427
Msa1294110	MtrunA17_Chr2g0328171	51.122	401	152	4	15	411	8	368	7.05e-146	421
Msa1294110	MtrunA17_Chr5g0416311	56.044	364	126	2	55	418	46	375	1.26e-144	418
Msa1294110	MtrunA17_Chr5g0435211	53.672	354	132	1	56	409	46	367	4.85e-144	417
Msa1294110	MtrunA17_Chr5g0448661	58.120	351	110	2	59	409	79	392	7.87e-143	413
Msa1294110	MtrunA17_Chr3g0111631	51.351	370	143	2	62	431	53	385	1.99e-138	402
Msa1294110	MtrunA17_Chr7g0238671	53.672	354	132	1	62	415	50	371	2.00e-137	400

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294110	AT4G35600.2	58.270	393	117	3	21	413	35	380	1.76e-164	469
Msa1294110	AT4G35600.1	58.270	393	117	3	21	413	34	379	1.92e-164	469
Msa1294110	AT2G17220.2	61.892	370	107	2	60	429	61	396	3.09e-164	468
Msa1294110	AT2G17220.1	61.892	370	107	2	60	429	62	397	3.42e-164	468
Msa1294110	AT2G28930.2	56.034	348	121	1	58	405	42	357	9.55e-145	419
Msa1294110	AT1G07570.1	56.000	350	122	1	56	405	39	356	1.15e-144	419
Msa1294110	AT1G07570.5	56.000	350	122	1	56	405	39	356	1.15e-144	419
Msa1294110	AT1G07570.2	56.000	350	122	1	56	405	39	356	1.15e-144	419
Msa1294110	AT2G28930.1	56.034	348	121	1	58	405	53	368	1.99e-144	419
Msa1294110	AT2G28930.3	56.034	348	121	1	58	405	45	360	2.03e-144	418

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Find 115 sgRNAs with CRISPR-Local

Find 831 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTTATAAAGGTTGGT+TGG	0.474192	8_3:+51820505	Msa1294110:CDS
AACGGTGGTGAGAGATGCTC+AGG	0.505869	8_3:+51812565	None:intergenic
AAGAGTGATGTGTATGGCTT+TGG	0.465372	8_3:+51824382	Msa1294110:CDS
AAGGAGTGGAGTGATAAGGG+TGG	0.628207	8_3:-51822695	None:intergenic
AAGGCTCTCTCACCCAAACT+TGG	0.506037	8_3:+51822015	Msa1294110:CDS
AAGGTTTCACCCATTCAACT+AGG	0.506065	8_3:-51824474	None:intergenic
AATAGAAAATATATATGGGA+AGG	0.578567	8_3:+51824788	Msa1294110:three_prime_UTR
AATGCGAAAATTTCAGATTT+CGG	0.282737	8_3:+51822870	Msa1294110:CDS
ACAACAAAACCTAGTTGAAT+GGG	0.498972	8_3:+51824465	Msa1294110:CDS
ACAAGAATCAAAATAGCTAT+TGG	0.431182	8_3:+51822257	Msa1294110:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCATTACAAATGTTAGAGA+CGG	-	chr8_3:51812852-51812871	None:intergenic	30.0%
TCTGACAATGTGTTATAAGT+GGG	+	chr8_3:51812919-51812938	Msa1294110:intron	30.0%
ATTGAAAAAATAGAGTGTGG+AGG	+	chr8_3:51813080-51813099	Msa1294110:intron	30.0%
AGAAAGATAGGAAATTCAGT+TGG	-	chr8_3:51813337-51813356	None:intergenic	30.0%
TTTGTTTCAGCTAATCTTGT+GGG	+	chr8_3:51813365-51813384	Msa1294110:intron	30.0%
TTACAAACTCTCCCTATAAA+AGG	+	chr8_3:51813489-51813508	Msa1294110:intron	30.0%
TGTAATTGAGTATTTGATGG+TGG	-	chr8_3:51813769-51813788	None:intergenic	30.0%
TAAAGGATGAATCGAGAATA+GGG	-	chr8_3:51813819-51813838	None:intergenic	30.0%
ATAAAGGATGAATCGAGAAT+AGG	-	chr8_3:51813820-51813839	None:intergenic	30.0%
TTCTAGATCTAGTGGTTTAA+AGG	-	chr8_3:51814099-51814118	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	51812579	51824975	51812579	ID=Msa1294110;Name=Msa1294110
chr8_3	mRNA	51812579	51824975	51812579	ID=Msa1294110-mRNA-1;Parent=Msa1294110;Name=Msa1294110-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0\|0.5\|0.71\|0.71\|1\|1\|7\|216\|433
chr8_3	exon	51812579	51812633	51812579	ID=Msa1294110-mRNA-1:exon:14576;Parent=Msa1294110-mRNA-1
chr8_3	exon	51820270	51820625	51820270	ID=Msa1294110-mRNA-1:exon:14577;Parent=Msa1294110-mRNA-1
chr8_3	exon	51821995	51822130	51821995	ID=Msa1294110-mRNA-1:exon:14578;Parent=Msa1294110-mRNA-1
chr8_3	exon	51822225	51822367	51822225	ID=Msa1294110-mRNA-1:exon:14579;Parent=Msa1294110-mRNA-1
chr8_3	exon	51822676	51822771	51822676	ID=Msa1294110-mRNA-1:exon:14580;Parent=Msa1294110-mRNA-1
chr8_3	exon	51822864	51822987	51822864	ID=Msa1294110-mRNA-1:exon:14581;Parent=Msa1294110-mRNA-1
chr8_3	exon	51824368	51824975	51824368	ID=Msa1294110-mRNA-1:exon:14582;Parent=Msa1294110-mRNA-1
chr8_3	CDS	51812579	51812633	51812579	ID=Msa1294110-mRNA-1:cds;Parent=Msa1294110-mRNA-1
chr8_3	CDS	51820270	51820625	51820270	ID=Msa1294110-mRNA-1:cds;Parent=Msa1294110-mRNA-1
chr8_3	CDS	51821995	51822130	51821995	ID=Msa1294110-mRNA-1:cds;Parent=Msa1294110-mRNA-1
chr8_3	CDS	51822225	51822367	51822225	ID=Msa1294110-mRNA-1:cds;Parent=Msa1294110-mRNA-1
chr8_3	CDS	51822676	51822771	51822676	ID=Msa1294110-mRNA-1:cds;Parent=Msa1294110-mRNA-1
chr8_3	CDS	51822864	51822987	51822864	ID=Msa1294110-mRNA-1:cds;Parent=Msa1294110-mRNA-1
chr8_3	CDS	51824368	51824759	51824368	ID=Msa1294110-mRNA-1:cds;Parent=Msa1294110-mRNA-1
chr8_3	three_prime_UTR	51824760	51824975	51824760	ID=Msa1294110-mRNA-1:three_prime_utr;Parent=Msa1294110-mRNA-1

Gene Sequence

>Msa1294110

ATGCTCAGGTACGGCGGCGATGAGTTCTCCGCAGTGAGACGAAAGGTTCCTGCAGGTTCAACAAGCACTGTTGGATTCTCAGCAACTACAAGCAGTGCTGGAAGAAGCCAATTCTCTGAGGTAGCAAGTGGAAGCATTGATGATACTAATGAAGGAGGGTCTCTTCCTTATCCTTCACCAAATGGACAAATTCTTGAAAGACCAAATTTGAAGGTTTTTAGTTTTATTGAATTGAAAGCTGCTACAAAAAGCTTCAAATCTGATACATTACTTGGTGAAGGTGGTTTTGGAAAAGTTTATAAAGGTTGGTTGGATGAGAAAACTCTTTCTCCTGCTAAAGCTGGTTCTGGAATGGTTGTTGCTATCAAAAAATTGAATTCTGAAAGTACTCAAGGTTTTCAAGAATGGCAGTCAGAAGTTAACTTCTTAGGAAGGCTCTCTCACCCAAACTTGGTGAAGCTATTAGGTTATTGTTGGGACGACGACGAGCTTCTTCTTGTCTACGAGTTCATGCCAAAAGGAAGTCTGGAGAATCATCTCTTCAGAAGAAATCCTAACATTGAACCACTTTCTTGGAACACAAGAATCAAAATAGCTATTGGTGCAGCTCGTGGTTTGGCTTTCTTGCATGATTCAGCACAGCAAGTCATATACAGAGATTTTAAGGCTTCAAATATACTCCTTGATGGGGTGAGAATCGAATCCGAGACCACCCTTATCACTCCACTCCTTAACTCTCCAACTTCAGCCATCAAGCCAGCCTTACATCCCCTAGAACTATCCGATAATTACAATGCGAAAATTTCAGATTTCGGATTAGCTAAATTGGGGCCTTCTGGGGGACAATCACATGTAACTACCAGGGTCATGGGGACTTATGGTTATGCTGCTCCAGAATACATTGCAACAGGGCACTTGTATGTGAAGAGTGATGTGTATGGCTTTGGTGTGGTGCTGTTAGAAATCCTAACAGCCATGAGGGCATTGGACACAAAGAGGCCAACAGGACAACAAAACCTAGTTGAATGGGTGAAACCTTTTCTCTCCAATAAGAAAAAATTGAAAGGTATAATGGATGCTAGAATAGAAGGACAATACTCACCAAAGGCAGCAGTACAAGCAGCAGCACTTTCTCTAAAGTGCTTAGAAAATGACCCTAAACAACGTCCTTCTATGAAAGAAGTACTTGAATCATTGGAAGCTATTGAAGCAATTCAGGTCAAAACCAAAAGATCCAACAACAATTCTCATCAACCTCCAGTTCTTCAAGCTGCTAGGCACCAGAGAGTTGTGAAAGTATAA

Protein sequence

>Msa1294110

MLRYGGDEFSAVRRKVPAGSTSTVGFSATTSSAGRSQFSEVASGSIDDTNEGGSLPYPSPNGQILERPNLKVFSFIELKAATKSFKSDTLLGEGGFGKVYKGWLDEKTLSPAKAGSGMVVAIKKLNSESTQGFQEWQSEVNFLGRLSHPNLVKLLGYCWDDDELLLVYEFMPKGSLENHLFRRNPNIEPLSWNTRIKIAIGAARGLAFLHDSAQQVIYRDFKASNILLDGVRIESETTLITPLLNSPTSAIKPALHPLELSDNYNAKISDFGLAKLGPSGGQSHVTTRVMGTYGYAAPEYIATGHLYVKSDVYGFGVVLLEILTAMRALDTKRPTGQQNLVEWVKPFLSNKKKLKGIMDARIEGQYSPKAAVQAAALSLKCLENDPKQRPSMKEVLESLEAIEAIQVKTKRSNNNSHQPPVLQAARHQRVVKV*