AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1295750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1295750 MtrunA17_Chr8g0373361 55.385 195 51 5 9 172 2 191 4.80e-57 184
Msa1295750 MtrunA17_Chr8g0373001 55.789 190 48 5 18 172 1 189 6.30e-57 184
Msa1295750 MtrunA17_Chr5g0404321 46.875 192 64 5 18 172 1 191 1.16e-46 158
Msa1295750 MtrunA17_Chr8g0373331 65.625 128 18 1 18 119 1 128 1.47e-45 153
Msa1295750 MtrunA17_Chr1g0155301 35.808 229 94 6 18 193 1 229 3.79e-31 117
Msa1295750 MtrunA17_Chr1g0156151 36.150 213 99 5 18 193 1 213 3.70e-30 110
Msa1295750 MtrunA17_Chr8g0373341 44.149 188 55 6 18 176 1 167 9.20e-28 106
Msa1295750 MtrunA17_Chr1g0196071 40.288 139 73 3 41 172 51 186 2.52e-22 92.8
Msa1295750 MtrunA17_Chr2g0286441 41.732 127 67 2 53 172 49 175 3.30e-22 92.4
Msa1295750 MtrunA17_Chr2g0278511 41.463 123 64 3 58 172 69 191 8.11e-22 91.7
Msa1295750 MtrunA17_Chr2g0315641 37.692 130 74 2 58 180 57 186 1.19e-20 87.8
Msa1295750 MtrunA17_Chr1g0201891 33.161 193 88 8 18 172 1 190 7.87e-19 83.2
Msa1295750 MtrunA17_Chr2g0315651 35.878 131 77 2 65 188 59 189 9.84e-18 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1295750 AT2G18280.2 42.408 191 67 5 18 172 1 184 2.44e-38 136
Msa1295750 AT2G18280.1 42.408 191 67 5 18 172 1 184 2.44e-38 136
Msa1295750 AT2G47900.2 34.375 192 86 5 18 172 1 189 3.18e-25 101
Msa1295750 AT2G47900.1 34.375 192 86 5 18 172 1 189 3.18e-25 101
Msa1295750 AT2G47900.3 34.197 193 86 6 18 172 1 190 1.18e-23 97.1
Msa1295750 AT1G47270.3 39.024 123 67 2 58 172 59 181 1.51e-23 96.7
Msa1295750 AT1G47270.1 39.024 123 67 2 58 172 84 206 1.61e-23 96.7
Msa1295750 AT1G47270.2 39.024 123 67 2 58 172 83 205 1.68e-23 96.7
Msa1295750 AT1G76900.2 35.065 154 79 3 58 190 72 225 4.48e-21 90.1
Msa1295750 AT1G76900.1 35.065 154 79 3 58 190 72 225 4.48e-21 90.1
Msa1295750 AT1G76900.3 35.065 154 79 3 58 190 72 225 4.48e-21 90.1
Msa1295750 AT1G25280.1 41.129 124 63 3 58 172 74 196 2.24e-20 88.2
Msa1295750 AT1G43640.2 37.398 123 69 2 58 172 4 126 2.17e-19 84.7
Msa1295750 AT1G43640.1 37.398 123 69 2 58 172 70 192 3.14e-19 84.7
Msa1295750 AT3G06380.1 37.931 116 63 3 65 172 56 170 1.01e-17 80.1
Msa1295750 AT1G53320.1 33.846 130 79 2 58 180 59 188 1.86e-16 76.6
Msa1295750 AT5G18680.1 38.261 115 64 2 65 172 62 176 5.04e-16 75.5
Msa1295750 AT1G61940.1 51.786 56 27 0 54 109 9 64 1.37e-12 64.7
Msa1295750 AT1G61940.2 51.852 54 26 0 53 106 8 61 2.46e-11 61.6

Find 36 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACCTGCTTCAATGAAAT+AGG 0.248006 8_3:+53952205 None:intergenic
TGAAAAGCCAATAGAATAAA+AGG 0.260752 8_3:+53951072 None:intergenic
TGTATTTAAGCAGCCTGTTT+TGG 0.289734 8_3:-53951264 Msa1295750:intron
ATTCCTGTTGTACTTAGGTT+TGG 0.318942 8_3:-53952037 Msa1295750:CDS
TCAAGTTGTCAAGATTATAA+AGG 0.338393 8_3:+53951043 None:intergenic
TTTACCAACATATTTGTTGC+TGG 0.356694 8_3:+53951132 None:intergenic
TTAGAAGAGTGCATTTAGAT+TGG 0.371475 8_3:-53952587 None:intergenic
AATTACAACCAAACCAAAAC+AGG 0.408310 8_3:+53951251 None:intergenic
CAAGTTGTCAAGATTATAAA+GGG 0.419405 8_3:+53951044 None:intergenic
ACAAATATGTTGGTAAAGTC+AGG 0.442655 8_3:-53951126 Msa1295750:CDS
ATTTCCTATTTCATTGAAGC+AGG 0.465510 8_3:-53952209 Msa1295750:intron
TCTACATTCCTGTTGTACTT+AGG 0.466375 8_3:-53952042 Msa1295750:CDS
GCTCCAAACCTAAGTACAAC+AGG 0.478489 8_3:+53952034 None:intergenic
TTGAAGAGAGTGAGACATCT+TGG 0.482210 8_3:-53952333 Msa1295750:CDS
GTCCGATCTTGTCATATCCT+TGG 0.492960 8_3:-53951176 Msa1295750:CDS
AAAGACAACAACTGCACGGG+CGG 0.494992 8_3:+53952309 None:intergenic
GACAAAGTCCCATGTTGCAC+TGG 0.508206 8_3:-53952437 Msa1295750:CDS
AAGACAACAACTGCACGGGC+GGG 0.537687 8_3:+53952310 None:intergenic
TGTCCGACAGAGCCTATGCT+TGG 0.543813 8_3:+53952456 None:intergenic
AGCATAGTCTGCAAACTCAA+GGG 0.565579 8_3:-53952520 Msa1295750:CDS
CAAGCCAGCAACAAATATGT+TGG 0.565774 8_3:-53951136 Msa1295750:CDS
ACAAAAGACAACAACTGCAC+GGG 0.567285 8_3:+53952306 None:intergenic
CACAAAAGACAACAACTGCA+CGG 0.575702 8_3:+53952305 None:intergenic
GTGCTTCGGTATGTAAATCG+TGG 0.582590 8_3:-53952285 Msa1295750:CDS
AAGCATAGTCTGCAAACTCA+AGG 0.583651 8_3:-53952521 Msa1295750:CDS
AGACCAAGCATAGGCTCTGT+CGG 0.586519 8_3:-53952459 Msa1295750:CDS
TTGTCAAGATTATAAAGGGA+TGG 0.589133 8_3:+53951048 None:intergenic
GAGTTACATCCAGTGCAACA+TGG 0.593872 8_3:+53952428 None:intergenic
CTTGACAATCTCTAACGTGA+TGG 0.598262 8_3:+53952258 None:intergenic
TGCCAAGGATATGACAAGAT+CGG 0.607478 8_3:+53951174 None:intergenic
AGTTACATCCAGTGCAACAT+GGG 0.608162 8_3:+53952429 None:intergenic
GCATAGTCTGCAAACTCAAG+GGG 0.643365 8_3:-53952519 Msa1295750:CDS
GTCAAGACTCCTCAGCAATG+TGG 0.658064 8_3:-53952241 Msa1295750:CDS
CTTCGGTATGTAAATCGTGG+AGG 0.672621 8_3:-53952282 Msa1295750:CDS
GTAAGCGTTCCACATTGCTG+AGG 0.696256 8_3:+53952232 None:intergenic
AGAGTGCTGAGACCAAGCAT+AGG 0.696911 8_3:-53952468 Msa1295750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGTATAGGTTTTACTTT+TGG - chr8_3:53952054-53952073 Msa1295750:CDS 20.0%
!!! ATTTATTTTCATTTAGGTTG+TGG - chr8_3:53952299-53952318 Msa1295750:CDS 20.0%
!!! GATTTATCCTTTTATTCTAT+TGG - chr8_3:53952561-53952580 Msa1295750:CDS 20.0%
!!! AAATGTATAGGTTTTACTTT+TGG - chr8_3:53952054-53952073 Msa1295750:CDS 20.0%
!!! ATTTATTTTCATTTAGGTTG+TGG - chr8_3:53952299-53952318 Msa1295750:CDS 20.0%
!!! GATTTATCCTTTTATTCTAT+TGG - chr8_3:53952561-53952580 Msa1295750:CDS 20.0%
! ATTATATCCAAAAGCAATTC+AGG + chr8_3:53951279-53951298 None:intergenic 25.0%
! TACAAATTATTCGTATTGCT+CGG - chr8_3:53951485-53951504 Msa1295750:intron 25.0%
!! ATCTGATACAATGCTTTATT+AGG - chr8_3:53951560-53951579 Msa1295750:intron 25.0%
! AAACAAAACCCAAAAACTTA+CGG + chr8_3:53951753-53951772 None:intergenic 25.0%
! GAATAAGATCTTAGATAACT+TGG - chr8_3:53951790-53951809 Msa1295750:intron 25.0%
!!! ATAATCAAGATAGCTGATTT+TGG + chr8_3:53951921-53951940 None:intergenic 25.0%
! TTCATCTATTAATGATCCAA+GGG + chr8_3:53951976-53951995 None:intergenic 25.0%
! ATTCATCTATTAATGATCCA+AGG + chr8_3:53951977-53951996 None:intergenic 25.0%
!!! GTTATTCTTGCAATTTGTTA+AGG - chr8_3:53952229-53952248 Msa1295750:CDS 25.0%
! TGAAAAGCCAATAGAATAAA+AGG + chr8_3:53952571-53952590 None:intergenic 25.0%
! ATTATATCCAAAAGCAATTC+AGG + chr8_3:53951279-53951298 None:intergenic 25.0%
! TACAAATTATTCGTATTGCT+CGG - chr8_3:53951485-53951504 Msa1295750:intron 25.0%
!! ATCTGATACAATGCTTTATT+AGG - chr8_3:53951560-53951579 Msa1295750:intron 25.0%
! AAACAAAACCCAAAAACTTA+CGG + chr8_3:53951753-53951772 None:intergenic 25.0%
! GAATAAGATCTTAGATAACT+TGG - chr8_3:53951790-53951809 Msa1295750:intron 25.0%
!!! ATAATCAAGATAGCTGATTT+TGG + chr8_3:53951921-53951940 None:intergenic 25.0%
! TTCATCTATTAATGATCCAA+GGG + chr8_3:53951976-53951995 None:intergenic 25.0%
! ATTCATCTATTAATGATCCA+AGG + chr8_3:53951977-53951996 None:intergenic 25.0%
!!! GTTATTCTTGCAATTTGTTA+AGG - chr8_3:53952229-53952248 Msa1295750:CDS 25.0%
! TGAAAAGCCAATAGAATAAA+AGG + chr8_3:53952571-53952590 None:intergenic 25.0%
ATTTCCTATTTCATTGAAGC+AGG - chr8_3:53951431-53951450 Msa1295750:intron 30.0%
ATTACCTGCTTCAATGAAAT+AGG + chr8_3:53951438-53951457 None:intergenic 30.0%
TTTGTATCTAACCAGAAAGT+GGG + chr8_3:53951471-53951490 None:intergenic 30.0%
ATTTGTATCTAACCAGAAAG+TGG + chr8_3:53951472-53951491 None:intergenic 30.0%
CATTGTATCAGATAATCACT+GGG + chr8_3:53951553-53951572 None:intergenic 30.0%
! TGATACAATGCTTTATTAGG+AGG - chr8_3:53951563-53951582 Msa1295750:intron 30.0%
TCAGCAATGAAATACTTACA+TGG + chr8_3:53951631-53951650 None:intergenic 30.0%
CATTGTCTTAGAAAAAGAGA+TGG + chr8_3:53951660-53951679 None:intergenic 30.0%
! CTTACTTACCGTAAGTTTTT+GGG - chr8_3:53951742-53951761 Msa1295750:intron 30.0%
! TTACATGTTCTAAACTGAGA+AGG + chr8_3:53951874-53951893 None:intergenic 30.0%
! TTCTCAGTTTAGAACATGTA+AGG - chr8_3:53951873-53951892 Msa1295750:intron 30.0%
! GTTACTTGCTCTAAATGTAT+AGG - chr8_3:53952042-53952061 Msa1295750:CDS 30.0%
AATTACAACCAAACCAAAAC+AGG + chr8_3:53952392-53952411 None:intergenic 30.0%
TATTAGCTGCCAAAAAGATA+AGG - chr8_3:53952442-53952461 Msa1295750:CDS 30.0%
! TTTACCAACATATTTGTTGC+TGG + chr8_3:53952511-53952530 None:intergenic 30.0%
! ACAAATATGTTGGTAAAGTC+AGG - chr8_3:53952514-53952533 Msa1295750:CDS 30.0%
ATTTCCTATTTCATTGAAGC+AGG - chr8_3:53951431-53951450 Msa1295750:intron 30.0%
ATTACCTGCTTCAATGAAAT+AGG + chr8_3:53951438-53951457 None:intergenic 30.0%
TTTGTATCTAACCAGAAAGT+GGG + chr8_3:53951471-53951490 None:intergenic 30.0%
ATTTGTATCTAACCAGAAAG+TGG + chr8_3:53951472-53951491 None:intergenic 30.0%
CATTGTATCAGATAATCACT+GGG + chr8_3:53951553-53951572 None:intergenic 30.0%
! TGATACAATGCTTTATTAGG+AGG - chr8_3:53951563-53951582 Msa1295750:intron 30.0%
TCAGCAATGAAATACTTACA+TGG + chr8_3:53951631-53951650 None:intergenic 30.0%
CATTGTCTTAGAAAAAGAGA+TGG + chr8_3:53951660-53951679 None:intergenic 30.0%
! CTTACTTACCGTAAGTTTTT+GGG - chr8_3:53951742-53951761 Msa1295750:intron 30.0%
! TTACATGTTCTAAACTGAGA+AGG + chr8_3:53951874-53951893 None:intergenic 30.0%
! TTCTCAGTTTAGAACATGTA+AGG - chr8_3:53951873-53951892 Msa1295750:intron 30.0%
! GTTACTTGCTCTAAATGTAT+AGG - chr8_3:53952042-53952061 Msa1295750:CDS 30.0%
AATTACAACCAAACCAAAAC+AGG + chr8_3:53952392-53952411 None:intergenic 30.0%
TATTAGCTGCCAAAAAGATA+AGG - chr8_3:53952442-53952461 Msa1295750:CDS 30.0%
! TTTACCAACATATTTGTTGC+TGG + chr8_3:53952511-53952530 None:intergenic 30.0%
! ACAAATATGTTGGTAAAGTC+AGG - chr8_3:53952514-53952533 Msa1295750:CDS 30.0%
AAACTCAAGGGGATGAAAAA+TGG - chr8_3:53951132-53951151 Msa1295750:CDS 35.0%
ATATCCAAAAGCAATTCAGG+TGG + chr8_3:53951276-53951295 None:intergenic 35.0%
! GCTTTTGGATATAATCCGTA+AGG - chr8_3:53951284-53951303 Msa1295750:intron 35.0%
! AGTTGTTGTCTTTTGTGCTT+CGG - chr8_3:53951341-53951360 Msa1295750:intron 35.0%
GCATTGTATCAGATAATCAC+TGG + chr8_3:53951554-53951573 None:intergenic 35.0%
TCTACATTCCTGTTGTACTT+AGG - chr8_3:53951598-53951617 Msa1295750:intron 35.0%
ATTCCTGTTGTACTTAGGTT+TGG - chr8_3:53951603-53951622 Msa1295750:intron 35.0%
! GCTTACTTACCGTAAGTTTT+TGG - chr8_3:53951741-53951760 Msa1295750:intron 35.0%
!!! CAAGATAGCTGATTTTGGTT+TGG + chr8_3:53951916-53951935 None:intergenic 35.0%
ATTAAAAGATTTCGAGCCCT+TGG - chr8_3:53951957-53951976 Msa1295750:intron 35.0%
TAAGCAGACAGTTACACAAA+CGG + chr8_3:53952186-53952205 None:intergenic 35.0%
! TGTATTTAAGCAGCCTGTTT+TGG - chr8_3:53952376-53952395 Msa1295750:intron 35.0%
! AAGATCGGACCTTATCTTTT+TGG + chr8_3:53952454-53952473 None:intergenic 35.0%
AAACTCAAGGGGATGAAAAA+TGG - chr8_3:53951132-53951151 Msa1295750:CDS 35.0%
ATATCCAAAAGCAATTCAGG+TGG + chr8_3:53951276-53951295 None:intergenic 35.0%
! GCTTTTGGATATAATCCGTA+AGG - chr8_3:53951284-53951303 Msa1295750:intron 35.0%
! AGTTGTTGTCTTTTGTGCTT+CGG - chr8_3:53951341-53951360 Msa1295750:intron 35.0%
GCATTGTATCAGATAATCAC+TGG + chr8_3:53951554-53951573 None:intergenic 35.0%
TCTACATTCCTGTTGTACTT+AGG - chr8_3:53951598-53951617 Msa1295750:intron 35.0%
ATTCCTGTTGTACTTAGGTT+TGG - chr8_3:53951603-53951622 Msa1295750:intron 35.0%
! GCTTACTTACCGTAAGTTTT+TGG - chr8_3:53951741-53951760 Msa1295750:intron 35.0%
!!! CAAGATAGCTGATTTTGGTT+TGG + chr8_3:53951916-53951935 None:intergenic 35.0%
ATTAAAAGATTTCGAGCCCT+TGG - chr8_3:53951957-53951976 Msa1295750:intron 35.0%
TAAGCAGACAGTTACACAAA+CGG + chr8_3:53952186-53952205 None:intergenic 35.0%
! TGTATTTAAGCAGCCTGTTT+TGG - chr8_3:53952376-53952395 Msa1295750:intron 35.0%
! AAGATCGGACCTTATCTTTT+TGG + chr8_3:53952454-53952473 None:intergenic 35.0%
AAGCATAGTCTGCAAACTCA+AGG - chr8_3:53951119-53951138 Msa1295750:CDS 40.0%
AGCATAGTCTGCAAACTCAA+GGG - chr8_3:53951120-53951139 Msa1295750:CDS 40.0%
! AAGGGGATGAAAAATGGACT+AGG - chr8_3:53951138-53951157 Msa1295750:CDS 40.0%
AGTTACATCCAGTGCAACAT+GGG + chr8_3:53951214-53951233 None:intergenic 40.0%
TCCAATTGAACCCATTCAAC+AGG - chr8_3:53951233-53951252 Msa1295750:CDS 40.0%
CCAATTGAACCCATTCAACA+GGG - chr8_3:53951234-53951253 Msa1295750:CDS 40.0%
! TCTACCACCTGAATTGCTTT+TGG - chr8_3:53951269-53951288 Msa1295750:intron 40.0%
TCTCACTCTCTTCAACCTTA+CGG + chr8_3:53951302-53951321 None:intergenic 40.0%
TTGAAGAGAGTGAGACATCT+TGG - chr8_3:53951307-53951326 Msa1295750:intron 40.0%
ACAAAAGACAACAACTGCAC+GGG + chr8_3:53951337-53951356 None:intergenic 40.0%
CACAAAAGACAACAACTGCA+CGG + chr8_3:53951338-53951357 None:intergenic 40.0%
CTTGACAATCTCTAACGTGA+TGG + chr8_3:53951385-53951404 None:intergenic 40.0%
TGAATTCTTCTCCCACTTTC+TGG - chr8_3:53951457-53951476 Msa1295750:intron 40.0%
TATCAGATAATCACTGGGAC+CGG + chr8_3:53951548-53951567 None:intergenic 40.0%
!! TTTGGTTTGGCTAGTGAGTT+AGG + chr8_3:53951903-53951922 None:intergenic 40.0%
AACCACACTTTCTTTCTGCA+AGG + chr8_3:53952101-53952120 None:intergenic 40.0%
CATATATGAGCCAGAGCAAA+CGG + chr8_3:53952157-53952176 None:intergenic 40.0%
!!! TTAAGCAGCCTGTTTTGGTT+TGG - chr8_3:53952381-53952400 Msa1295750:intron 40.0%
TGCCAAGGATATGACAAGAT+CGG + chr8_3:53952469-53952488 None:intergenic 40.0%
ACAAAAATCGTCTGTTGCCA+AGG + chr8_3:53952484-53952503 None:intergenic 40.0%
CAAGCCAGCAACAAATATGT+TGG - chr8_3:53952504-53952523 Msa1295750:CDS 40.0%
AAGCATAGTCTGCAAACTCA+AGG - chr8_3:53951119-53951138 Msa1295750:CDS 40.0%
AGCATAGTCTGCAAACTCAA+GGG - chr8_3:53951120-53951139 Msa1295750:CDS 40.0%
! AAGGGGATGAAAAATGGACT+AGG - chr8_3:53951138-53951157 Msa1295750:CDS 40.0%
AGTTACATCCAGTGCAACAT+GGG + chr8_3:53951214-53951233 None:intergenic 40.0%
TCCAATTGAACCCATTCAAC+AGG - chr8_3:53951233-53951252 Msa1295750:CDS 40.0%
CCAATTGAACCCATTCAACA+GGG - chr8_3:53951234-53951253 Msa1295750:CDS 40.0%
! TCTACCACCTGAATTGCTTT+TGG - chr8_3:53951269-53951288 Msa1295750:intron 40.0%
TCTCACTCTCTTCAACCTTA+CGG + chr8_3:53951302-53951321 None:intergenic 40.0%
TTGAAGAGAGTGAGACATCT+TGG - chr8_3:53951307-53951326 Msa1295750:intron 40.0%
ACAAAAGACAACAACTGCAC+GGG + chr8_3:53951337-53951356 None:intergenic 40.0%
CACAAAAGACAACAACTGCA+CGG + chr8_3:53951338-53951357 None:intergenic 40.0%
CTTGACAATCTCTAACGTGA+TGG + chr8_3:53951385-53951404 None:intergenic 40.0%
TGAATTCTTCTCCCACTTTC+TGG - chr8_3:53951457-53951476 Msa1295750:intron 40.0%
TATCAGATAATCACTGGGAC+CGG + chr8_3:53951548-53951567 None:intergenic 40.0%
!! TTTGGTTTGGCTAGTGAGTT+AGG + chr8_3:53951903-53951922 None:intergenic 40.0%
AACCACACTTTCTTTCTGCA+AGG + chr8_3:53952101-53952120 None:intergenic 40.0%
CATATATGAGCCAGAGCAAA+CGG + chr8_3:53952157-53952176 None:intergenic 40.0%
!!! TTAAGCAGCCTGTTTTGGTT+TGG - chr8_3:53952381-53952400 Msa1295750:intron 40.0%
TGCCAAGGATATGACAAGAT+CGG + chr8_3:53952469-53952488 None:intergenic 40.0%
ACAAAAATCGTCTGTTGCCA+AGG + chr8_3:53952484-53952503 None:intergenic 40.0%
CAAGCCAGCAACAAATATGT+TGG - chr8_3:53952504-53952523 Msa1295750:CDS 40.0%
GCATAGTCTGCAAACTCAAG+GGG - chr8_3:53951121-53951140 Msa1295750:CDS 45.0%
GAGTTACATCCAGTGCAACA+TGG + chr8_3:53951215-53951234 None:intergenic 45.0%
! CCCTGTTGAATGGGTTCAAT+TGG + chr8_3:53951237-53951256 None:intergenic 45.0%
AACCCATTCAACAGGGACAA+TGG - chr8_3:53951241-53951260 Msa1295750:CDS 45.0%
ACCCATTCAACAGGGACAAT+GGG - chr8_3:53951242-53951261 Msa1295750:CDS 45.0%
GTGCTTCGGTATGTAAATCG+TGG - chr8_3:53951355-53951374 Msa1295750:intron 45.0%
CTTCGGTATGTAAATCGTGG+AGG - chr8_3:53951358-53951377 Msa1295750:intron 45.0%
ATCAGATAATCACTGGGACC+GGG + chr8_3:53951547-53951566 None:intergenic 45.0%
GCTCCAAACCTAAGTACAAC+AGG + chr8_3:53951609-53951628 None:intergenic 45.0%
AGCCTTGCAGAAAGAAAGTG+TGG - chr8_3:53952096-53952115 Msa1295750:intron 45.0%
GTCCGATCTTGTCATATCCT+TGG - chr8_3:53952464-53952483 Msa1295750:CDS 45.0%
GCATAGTCTGCAAACTCAAG+GGG - chr8_3:53951121-53951140 Msa1295750:CDS 45.0%
GAGTTACATCCAGTGCAACA+TGG + chr8_3:53951215-53951234 None:intergenic 45.0%
! CCCTGTTGAATGGGTTCAAT+TGG + chr8_3:53951237-53951256 None:intergenic 45.0%
AACCCATTCAACAGGGACAA+TGG - chr8_3:53951241-53951260 Msa1295750:CDS 45.0%
ACCCATTCAACAGGGACAAT+GGG - chr8_3:53951242-53951261 Msa1295750:CDS 45.0%
GTGCTTCGGTATGTAAATCG+TGG - chr8_3:53951355-53951374 Msa1295750:intron 45.0%
CTTCGGTATGTAAATCGTGG+AGG - chr8_3:53951358-53951377 Msa1295750:intron 45.0%
ATCAGATAATCACTGGGACC+GGG + chr8_3:53951547-53951566 None:intergenic 45.0%
GCTCCAAACCTAAGTACAAC+AGG + chr8_3:53951609-53951628 None:intergenic 45.0%
AGCCTTGCAGAAAGAAAGTG+TGG - chr8_3:53952096-53952115 Msa1295750:intron 45.0%
GTCCGATCTTGTCATATCCT+TGG - chr8_3:53952464-53952483 Msa1295750:CDS 45.0%
!!! TTATTTATTTATTTTCATTT+AGG - chr8_3:53952293-53952312 Msa1295750:CDS 5.0%
!!! TTATTTATTTATTTTCATTT+AGG - chr8_3:53952293-53952312 Msa1295750:CDS 5.0%
! TTTTGTGTTGCGACGCAGTG+TGG - chr8_3:53951081-53951100 Msa1295750:CDS 50.0%
!! AGAGTGCTGAGACCAAGCAT+AGG - chr8_3:53951172-53951191 Msa1295750:CDS 50.0%
AGACCAAGCATAGGCTCTGT+CGG - chr8_3:53951181-53951200 Msa1295750:CDS 50.0%
GACAAAGTCCCATGTTGCAC+TGG - chr8_3:53951203-53951222 Msa1295750:CDS 50.0%
GCCCATTGTCCCTGTTGAAT+GGG + chr8_3:53951246-53951265 None:intergenic 50.0%
TGCCCATTGTCCCTGTTGAA+TGG + chr8_3:53951247-53951266 None:intergenic 50.0%
AAAGACAACAACTGCACGGG+CGG + chr8_3:53951334-53951353 None:intergenic 50.0%
GTCAAGACTCCTCAGCAATG+TGG - chr8_3:53951399-53951418 Msa1295750:intron 50.0%
GTAAGCGTTCCACATTGCTG+AGG + chr8_3:53951411-53951430 None:intergenic 50.0%
! CTTTATGCGTGTGACTAGCC+CGG - chr8_3:53951526-53951545 Msa1295750:intron 50.0%
CAGAGCAAACGGTTACAAGC+GGG + chr8_3:53952146-53952165 None:intergenic 50.0%
CCAGAGCAAACGGTTACAAG+CGG + chr8_3:53952147-53952166 None:intergenic 50.0%
! TTTTGTGTTGCGACGCAGTG+TGG - chr8_3:53951081-53951100 Msa1295750:CDS 50.0%
!! AGAGTGCTGAGACCAAGCAT+AGG - chr8_3:53951172-53951191 Msa1295750:CDS 50.0%
AGACCAAGCATAGGCTCTGT+CGG - chr8_3:53951181-53951200 Msa1295750:CDS 50.0%
GACAAAGTCCCATGTTGCAC+TGG - chr8_3:53951203-53951222 Msa1295750:CDS 50.0%
GCCCATTGTCCCTGTTGAAT+GGG + chr8_3:53951246-53951265 None:intergenic 50.0%
TGCCCATTGTCCCTGTTGAA+TGG + chr8_3:53951247-53951266 None:intergenic 50.0%
AAAGACAACAACTGCACGGG+CGG + chr8_3:53951334-53951353 None:intergenic 50.0%
GTCAAGACTCCTCAGCAATG+TGG - chr8_3:53951399-53951418 Msa1295750:intron 50.0%
GTAAGCGTTCCACATTGCTG+AGG + chr8_3:53951411-53951430 None:intergenic 50.0%
! CTTTATGCGTGTGACTAGCC+CGG - chr8_3:53951526-53951545 Msa1295750:intron 50.0%
CAGAGCAAACGGTTACAAGC+GGG + chr8_3:53952146-53952165 None:intergenic 50.0%
CCAGAGCAAACGGTTACAAG+CGG + chr8_3:53952147-53952166 None:intergenic 50.0%
TGTCCGACAGAGCCTATGCT+TGG + chr8_3:53951187-53951206 None:intergenic 55.0%
AAGACAACAACTGCACGGGC+GGG + chr8_3:53951333-53951352 None:intergenic 55.0%
!! CGGGCTAGTCACACGCATAA+AGG + chr8_3:53951528-53951547 None:intergenic 55.0%
CCGCTTGTAACCGTTTGCTC+TGG - chr8_3:53952144-53952163 Msa1295750:intron 55.0%
TGTCCGACAGAGCCTATGCT+TGG + chr8_3:53951187-53951206 None:intergenic 55.0%
AAGACAACAACTGCACGGGC+GGG + chr8_3:53951333-53951352 None:intergenic 55.0%
!! CGGGCTAGTCACACGCATAA+AGG + chr8_3:53951528-53951547 None:intergenic 55.0%
CCGCTTGTAACCGTTTGCTC+TGG - chr8_3:53952144-53952163 Msa1295750:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 53951057 53952605 53951057 ID=Msa1295750;Name=Msa1295750
chr8_3 mRNA 53951057 53952605 53951057 ID=Msa1295750-mRNA-1;Parent=Msa1295750;Name=Msa1295750-mRNA-1;_AED=0.44;_eAED=0.49;_QI=0|0|0|1|0.33|0.25|4|0|194
chr8_3 exon 53952435 53952605 53952435 ID=Msa1295750-mRNA-1:exon:15577;Parent=Msa1295750-mRNA-1
chr8_3 exon 53952210 53952356 53952210 ID=Msa1295750-mRNA-1:exon:15576;Parent=Msa1295750-mRNA-1
chr8_3 exon 53952031 53952080 53952031 ID=Msa1295750-mRNA-1:exon:15575;Parent=Msa1295750-mRNA-1
chr8_3 exon 53951057 53951273 53951057 ID=Msa1295750-mRNA-1:exon:15574;Parent=Msa1295750-mRNA-1
chr8_3 CDS 53952435 53952605 53952435 ID=Msa1295750-mRNA-1:cds;Parent=Msa1295750-mRNA-1
chr8_3 CDS 53952210 53952356 53952210 ID=Msa1295750-mRNA-1:cds;Parent=Msa1295750-mRNA-1
chr8_3 CDS 53952031 53952080 53952031 ID=Msa1295750-mRNA-1:cds;Parent=Msa1295750-mRNA-1
chr8_3 CDS 53951057 53951273 53951057 ID=Msa1295750-mRNA-1:cds;Parent=Msa1295750-mRNA-1
Gene Sequence

>Msa1295750

AAGAGTGCATTTAGATTGGAGCAGTTTTGTGTTGCGACGCAGTGTGGAAACATGTCATTGAAAAGCATAGTCTGCAAACTCAAGGGGATGAAAAATGGACTAGGTAGCATATCAAAGAGTGCTGAGACCAAGCATAGGCTCTGTCGGACAAAGTCCCATGTTGCACTGGATGTTGAAGAGAGTGAGACATCTTGGCCCGCCCGTGCAGTTGTTGTCTTTTGTGCTTCGGTATGTAAATCGTGGAGGTCCATCACGTTAGAGATTGTCAAGACTCCTCAGCAATGTGGAACGCTTACATTTCCTATTTCATTGAAGCAGGAGGAACAAAAGAACGTCTACATTCCTGTTGTACTTAGGTTTGGAGCCATCCTGTTTTGGTTTGGTTGTAATTTTATAGCGAAGAATGAGAGTAATAAGTTGTTATTAGCTGCCAAAAAGATAAGGTCCGATCTTGTCATATCCTTGGCAACAGACGATTTTTGTCAAGCCAGCAACAAATATGTTGGTAAAGTCAGGCAAGTTTTAGTCAATAGAAACATTGATTTATCCTTTTATTCTATTGGCTTTTCATCCATCCCTTTATAA
Protein sequence

>Msa1295750

KSAFRLEQFCVATQCGNMSLKSIVCKLKGMKNGLGSISKSAETKHRLCRTKSHVALDVEESETSWPARAVVVFCASVCKSWRSITLEIVKTPQQCGTLTFPISLKQEEQKNVYIPVVLRFGAILFWFGCNFIAKNESNKLLLAAKKIRSDLVISLATDDFCQASNKYVGKVRQVLVNRNIDLSFYSIGFSSIPL*