AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1295860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1295860 MtrunA17_Chr8g0373821 88.679 318 19 3 2 303 217 533 0.0 569
Msa1295860 MtrunA17_Chr5g0403241 60.784 306 95 5 23 303 1197 1502 3.18e-113 357
Msa1295860 MtrunA17_Chr4g0075321 62.813 320 93 7 8 303 742 1059 4.31e-112 348
Msa1295860 MtrunA17_Chr5g0399801 34.259 216 97 9 128 303 874 1084 2.05e-26 109
Msa1295860 MtrunA17_Chr4g0003501 33.333 213 101 7 128 303 742 950 7.19e-26 107
Msa1295860 MtrunA17_Chr4g0003511 33.488 215 100 9 128 303 837 1047 5.47e-25 105
Msa1295860 MtrunA17_Chr1g0183321 33.702 181 97 4 146 303 561 741 6.36e-24 102
Msa1295860 MtrunA17_Chr1g0190311 34.426 183 94 5 146 303 458 639 1.71e-23 100
Msa1295860 MtrunA17_Chr7g0262001 34.807 181 95 4 146 303 517 697 6.51e-23 99.0
Msa1295860 MtrunA17_Chr7g0255181 29.221 308 154 15 27 303 426 700 1.31e-22 98.2
Msa1295860 MtrunA17_Chr3g0080761 27.134 328 164 13 22 303 361 659 8.73e-19 86.7
Msa1295860 MtrunA17_Chr8g0354821 27.607 326 166 12 20 303 386 683 5.41e-18 84.3
Msa1295860 MtrunA17_Chr1g0170651 29.932 294 146 12 30 303 288 541 6.47e-18 84.0
Msa1295860 MtrunA17_Chr2g0305371 32.317 164 91 4 150 303 314 467 8.48e-16 77.4
Msa1295860 MtrunA17_Chr6g0477001 30.682 176 92 5 144 303 85 246 2.39e-15 75.5
Msa1295860 MtrunA17_Chr8g0368901 27.419 186 105 5 128 284 571 755 2.89e-14 73.2
Msa1295860 MtrunA17_Chr6g0476121 27.830 212 107 6 136 303 429 638 9.30e-14 71.6
Msa1295860 MtrunA17_Chr7g0252211 24.684 316 167 11 33 303 463 752 1.69e-13 70.9
Msa1295860 MtrunA17_Chr6g0476111 27.358 212 108 6 136 303 480 689 2.62e-13 70.5
Msa1295860 MtrunA17_Chr7g0232981 29.630 189 89 6 140 287 303 488 6.13e-13 68.9
Msa1295860 MtrunA17_Chr6g0460921 28.333 180 98 4 149 303 242 415 1.76e-12 67.4
Msa1295860 MtrunA17_Chr6g0467421 26.816 179 101 5 139 287 387 565 4.43e-12 66.6
Msa1295860 MtrunA17_Chr6g0467381 26.816 179 101 5 139 287 387 565 4.43e-12 66.6
Msa1295860 MtrunA17_Chr1g0201311 31.481 162 90 5 154 303 67 219 5.83e-12 65.9
Msa1295860 MtrunA17_Chr6g0467531 26.816 179 101 5 139 287 387 565 1.39e-11 65.1
Msa1295860 MtrunA17_Chr1g0201621 30.864 162 91 5 154 303 67 219 2.54e-11 63.9
Msa1295860 MtrunA17_Chr7g0232841 29.444 180 79 7 170 303 346 523 2.85e-11 63.9
Msa1295860 MtrunA17_Chr6g0467401 26.257 179 102 5 139 287 387 565 8.07e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1295860 AT2G23740.1 52.703 296 108 7 33 303 1082 1370 3.25e-83 273
Msa1295860 AT2G23740.3 52.703 296 108 7 33 303 1089 1377 3.51e-83 273
Msa1295860 AT2G23740.6 52.703 296 108 7 33 303 1089 1377 3.51e-83 273
Msa1295860 AT2G23740.2 52.703 296 108 7 33 303 1089 1377 3.51e-83 273
Msa1295860 AT2G23740.4 56.410 234 82 5 33 253 1089 1315 6.67e-72 241
Msa1295860 AT2G23740.5 56.410 234 82 5 33 253 1089 1315 6.67e-72 241
Msa1295860 AT3G04380.2 34.615 182 94 6 146 303 250 430 1.12e-24 103
Msa1295860 AT3G04380.5 34.615 182 94 6 146 303 234 414 1.15e-24 103
Msa1295860 AT3G04380.6 34.615 182 94 6 146 303 250 430 1.24e-24 103
Msa1295860 AT3G04380.4 34.615 182 94 6 146 303 277 457 1.43e-24 103
Msa1295860 AT3G04380.1 34.615 182 94 6 146 303 277 457 1.53e-24 103
Msa1295860 AT3G04380.3 34.066 182 95 5 146 303 261 441 1.69e-24 103
Msa1295860 AT1G73100.1 27.692 325 161 14 22 303 371 664 1.18e-23 101
Msa1295860 AT1G04050.3 34.637 179 93 5 147 303 438 614 2.80e-23 100
Msa1295860 AT1G04050.2 34.637 179 93 5 147 303 542 718 3.06e-23 100
Msa1295860 AT1G04050.1 34.637 179 93 5 147 303 542 718 3.06e-23 100
Msa1295860 AT2G22740.2 30.093 216 107 7 128 303 574 785 6.29e-21 93.6
Msa1295860 AT2G22740.1 30.093 216 107 7 128 303 574 785 6.29e-21 93.6
Msa1295860 AT5G43990.8 33.333 180 93 5 147 303 457 632 6.04e-20 90.5
Msa1295860 AT5G43990.12 33.333 180 93 5 147 303 457 632 6.04e-20 90.5
Msa1295860 AT5G43990.6 33.333 180 93 5 147 303 457 632 6.04e-20 90.5
Msa1295860 AT5G43990.13 33.333 180 93 5 147 303 457 632 6.04e-20 90.5
Msa1295860 AT5G43990.7 33.333 180 93 5 147 303 437 612 6.16e-20 90.5
Msa1295860 AT5G43990.9 33.333 180 93 5 147 303 549 724 6.42e-20 90.5
Msa1295860 AT5G43990.2 33.333 180 93 5 147 303 549 724 6.42e-20 90.5
Msa1295860 AT5G43990.3 33.333 180 93 5 147 303 526 701 6.52e-20 90.5
Msa1295860 AT5G43990.11 33.333 180 93 5 147 303 526 701 6.52e-20 90.5
Msa1295860 AT5G43990.1 33.333 180 93 5 147 303 526 701 6.52e-20 90.5
Msa1295860 AT5G43990.4 33.333 180 93 5 147 303 526 701 6.52e-20 90.5
Msa1295860 AT5G43990.15 33.333 180 93 5 147 303 529 704 6.59e-20 90.5
Msa1295860 AT5G43990.10 33.333 180 93 5 147 303 529 704 6.59e-20 90.5
Msa1295860 AT5G43990.16 33.333 180 93 5 147 303 506 681 7.48e-20 90.1
Msa1295860 AT5G43990.5 33.333 180 93 5 147 303 506 681 7.48e-20 90.1
Msa1295860 AT5G43990.17 33.333 180 93 5 147 303 506 681 7.48e-20 90.1
Msa1295860 AT5G43990.14 33.333 180 93 5 147 303 506 681 7.48e-20 90.1
Msa1295860 AT2G35160.5 31.551 187 92 6 140 303 580 753 1.84e-18 86.3
Msa1295860 AT2G35160.2 31.551 187 92 6 140 303 616 789 2.01e-18 86.3
Msa1295860 AT2G35160.3 31.551 187 92 6 140 303 616 789 2.01e-18 86.3
Msa1295860 AT2G35160.1 31.551 187 92 6 140 303 616 789 2.01e-18 86.3
Msa1295860 AT2G35160.4 31.551 187 92 6 140 303 616 789 2.01e-18 86.3
Msa1295860 AT5G13960.2 28.159 277 140 9 71 303 224 485 4.35e-17 81.6
Msa1295860 AT5G13960.1 28.159 277 140 9 71 303 358 619 7.92e-17 81.3
Msa1295860 AT3G03750.2 31.016 187 93 6 149 303 169 351 4.59e-15 75.1
Msa1295860 AT5G04940.2 25.617 324 168 14 22 303 373 665 1.31e-14 74.7
Msa1295860 AT5G04940.1 25.617 324 168 14 22 303 373 665 1.31e-14 74.7
Msa1295860 AT4G30860.1 30.488 164 94 4 150 303 307 460 2.01e-14 73.9
Msa1295860 AT1G17770.1 26.220 328 173 15 18 303 388 688 2.12e-14 73.9

Find 63 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATACATAATTTCCTTAT+TGG 0.135814 8_3:+54075305 None:intergenic
GATGAAGAAAAGGACATATT+TGG 0.136959 8_3:-54076941 Msa1295860:CDS
GGCGAAATGCTCAAGCTTTC+TGG 0.183364 8_3:-54077591 Msa1295860:exon
ATGAAGAAAAGGACATATTT+GGG 0.300996 8_3:-54076940 Msa1295860:CDS
GAACGGAGTTCGTGTTAAAT+TGG 0.304027 8_3:-54076537 Msa1295860:CDS
CCGTTCTGCAATATTCTGTT+TGG 0.323633 8_3:+54076554 None:intergenic
TATACGTGATCACACGTTTC+AGG 0.337540 8_3:+54076971 None:intergenic
CAACTTATTCCGCAATATAC+TGG 0.363464 8_3:+54075353 None:intergenic
TGTAAATGGTCGAATCATAT+TGG 0.379997 8_3:-54076888 Msa1295860:CDS
AGGTTCGCATACGATGTAAA+TGG 0.389210 8_3:-54076902 Msa1295860:CDS
AACATTTAAAGTGTCCAATA+AGG 0.399037 8_3:-54075319 Msa1295860:three_prime_UTR
ATTCACAGGGATTCGGAGTA+AGG 0.405434 8_3:-54076418 Msa1295860:intron
TATGAGCCTGTGCTCGTAGA+AGG 0.406674 8_3:-54075467 Msa1295860:CDS
TTCTTTGCAAAGAACATCTC+AGG 0.407939 8_3:-54077612 Msa1295860:five_prime_UTR
TGATAATGAGAATGCTGTTA+AGG 0.413807 8_3:-54077541 Msa1295860:CDS
TATGCGAACCTTCCGGCCAT+TGG 0.417185 8_3:+54076914 None:intergenic
TACATCGTATGCGAACCTTC+CGG 0.420636 8_3:+54076907 None:intergenic
TGTCTAGGTTCATCAATAAC+AGG 0.425670 8_3:-54076104 Msa1295860:intron
TTATCTGATTCACAGGGATT+CGG 0.425686 8_3:-54076425 Msa1295860:intron
TAGTAGAATCAATGACATAT+CGG 0.427363 8_3:+54076139 None:intergenic
GAAGGATATCCTTGTCTTTG+TGG 0.428480 8_3:-54075449 Msa1295860:CDS
TATGTATGTGTAACATTGAA+TGG 0.430897 8_3:-54075292 Msa1295860:three_prime_UTR
CTGCAATATTCTGTTTGGAC+AGG 0.439001 8_3:+54076559 None:intergenic
GTAAACATCTCCCAAGGCTT+AGG 0.442228 8_3:+54077381 None:intergenic
CTTCGATCAATCTACTCATA+TGG 0.456321 8_3:+54076169 None:intergenic
GTCATTGATTCTACTACATA+TGG 0.457853 8_3:-54076132 Msa1295860:CDS
TTGATTCTGCCGCAAAGCTT+AGG 0.472145 8_3:+54077475 None:intergenic
AGTAGAATCAATGACATATC+GGG 0.477925 8_3:+54076140 None:intergenic
CAGAGTTATTGTAGAGGGCA+AGG 0.479784 8_3:-54075705 Msa1295860:CDS
ATGCAATGACCTAAGCTTTG+CGG 0.481210 8_3:-54077484 Msa1295860:CDS
CTACGCAATTGATGAAGAAT+CGG 0.488801 8_3:+54077345 None:intergenic
ATGTATATCTTCCTAAGCCT+TGG 0.489667 8_3:-54077392 Msa1295860:CDS
TGTATATCTTCCTAAGCCTT+GGG 0.489888 8_3:-54077391 Msa1295860:CDS
GAGATGTTCTTTGCAAAGAA+AGG 0.490990 8_3:+54077615 None:intergenic
CTACATATGGCAATGTGTCT+AGG 0.499868 8_3:-54076119 Msa1295860:CDS
AAATGTTGAGGTTCTAGTAA+AGG 0.506407 8_3:+54075405 None:intergenic
GATTGTAAGCATACACATAT+CGG 0.512628 8_3:-54075683 Msa1295860:CDS
AAAATTAGGGTCGTCGCGAA+AGG 0.515962 8_3:-54077517 Msa1295860:CDS
CGGAGTTCGTGTTAAATTGG+AGG 0.527617 8_3:-54076534 Msa1295860:intron
CAGGGATTCGGAGTAAGGGC+TGG 0.542170 8_3:-54076413 Msa1295860:CDS
AAGAATCGGCTTTGTCACAT+AGG 0.562883 8_3:+54077359 None:intergenic
ATTGCGTAGTCTTGATTCTG+AGG 0.575859 8_3:-54077331 Msa1295860:intron
TTCACAGGGATTCGGAGTAA+GGG 0.591817 8_3:-54076417 Msa1295860:intron
GATTACAGAGTTATTGTAGA+GGG 0.592650 8_3:-54075710 Msa1295860:intron
GGATATCCTTCTACGAGCAC+AGG 0.594050 8_3:+54075461 None:intergenic
AAATGGTCGAATCATATTGG+AGG 0.599006 8_3:-54076885 Msa1295860:intron
CAGGAGGCACACAACAGACG+CGG 0.600597 8_3:-54076332 Msa1295860:CDS
TTAGACTGTCCAGTATATTG+CGG 0.600785 8_3:-54075362 Msa1295860:three_prime_UTR
TGTGGATCTTTGAAATGCTG+AGG 0.600856 8_3:-54075431 Msa1295860:exon
TTGGGAAACCAATGGCCGGA+AGG 0.605290 8_3:-54076922 Msa1295860:CDS
AATTATACTTCGATTCACAA+AGG 0.622152 8_3:+54075207 None:intergenic
ATATTTGGGAAACCAATGGC+CGG 0.622843 8_3:-54076926 Msa1295860:CDS
AGATCTACTACACATATCAC+AGG 0.627421 8_3:+54077450 None:intergenic
GGACATATTTGGGAAACCAA+TGG 0.633831 8_3:-54076930 Msa1295860:CDS
TTCAAAGATCCACAAAGACA+AGG 0.641053 8_3:+54075440 None:intergenic
TGATTACAGAGTTATTGTAG+AGG 0.655560 8_3:-54075711 Msa1295860:intron
ACGTTTGTGTGTGAGTACAT+TGG 0.657919 8_3:-54076371 Msa1295860:CDS
AGGCACACAACAGACGCGGA+AGG 0.658214 8_3:-54076328 Msa1295860:intron
GTAGAATCAATGACATATCG+GGG 0.662628 8_3:+54076141 None:intergenic
ACATAGGTAAACATCTCCCA+AGG 0.667902 8_3:+54077375 None:intergenic
CCAAACAGAATATTGCAGAA+CGG 0.677454 8_3:-54076554 Msa1295860:CDS
ACACATTTAACATCTCGTTG+AGG 0.700034 8_3:-54075238 Msa1295860:three_prime_UTR
TTCGAGTGTAACGATAAGTG+CGG 0.716852 8_3:-54076593 Msa1295860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGTAGTTTACAATTAA+TGG + chr8_3:54075918-54075937 None:intergenic 15.0%
!! TGGCATTTAAAAAGAAAAAT+TGG - chr8_3:54075647-54075666 Msa1295860:intron 20.0%
!!! ATTTTAGCATATTCTTGATA+TGG + chr8_3:54075989-54076008 None:intergenic 20.0%
!! GATAAGACAAAAAATTAACA+TGG - chr8_3:54076699-54076718 Msa1295860:intron 20.0%
!!! GCAATTTGTTCATTTTTAAT+TGG - chr8_3:54076723-54076742 Msa1295860:intron 20.0%
!!! CTACACATTTAATAACTTTT+TGG + chr8_3:54076836-54076855 None:intergenic 20.0%
!! ATAGACTACTTTAAACTTAA+CGG - chr8_3:54076918-54076937 Msa1295860:CDS 20.0%
!! TTGTATTTAACACATTAATC+AGG + chr8_3:54076945-54076964 None:intergenic 20.0%
! AATGCTGTTAAGGAAAAATT+AGG - chr8_3:54075236-54075255 Msa1295860:three_prime_UTR 25.0%
! ATGCTGTTAAGGAAAAATTA+GGG - chr8_3:54075237-54075256 Msa1295860:three_prime_UTR 25.0%
! ATGATCAAACTGTAATGATA+CGG - chr8_3:54075486-54075505 Msa1295860:CDS 25.0%
!!! TATGTAGTTGTGTTTTGTTA+AGG - chr8_3:54075573-54075592 Msa1295860:intron 25.0%
! AATAGCAGGTTAAATATTGT+CGG + chr8_3:54075724-54075743 None:intergenic 25.0%
!! ATACCTTTTTGATGAAGAAA+AGG - chr8_3:54075816-54075835 Msa1295860:intron 25.0%
! ATGAAGAAAAGGACATATTT+GGG - chr8_3:54075827-54075846 Msa1295860:intron 25.0%
!! TCCTAACATATTCATTTTGT+AGG - chr8_3:54076008-54076027 Msa1295860:intron 25.0%
! ACCTACAAAATGAATATGTT+AGG + chr8_3:54076012-54076031 None:intergenic 25.0%
! TGTAAGATGTAAATTCTACT+AGG + chr8_3:54076059-54076078 None:intergenic 25.0%
!!! TAACTAATTTTTTCCAATGC+TGG - chr8_3:54076114-54076133 Msa1295860:CDS 25.0%
! AAATTTCTCTACAAAACTTC+AGG - chr8_3:54076137-54076156 Msa1295860:CDS 25.0%
! GCTATATTTGGTATTAAAAC+AGG + chr8_3:54076534-54076553 None:intergenic 25.0%
!!! GCAATTTTCTTTGCTATATT+TGG + chr8_3:54076546-54076565 None:intergenic 25.0%
! TAGTAGAATCAATGACATAT+CGG + chr8_3:54076631-54076650 None:intergenic 25.0%
! TTATTTCCTTGAACATAAGA+TGG - chr8_3:54076768-54076787 Msa1295860:intron 25.0%
! GCTATTTACAACTAAATGAT+TGG - chr8_3:54076991-54077010 Msa1295860:CDS 25.0%
! ATTTACAACTAAATGATTGG+TGG - chr8_3:54076994-54077013 Msa1295860:CDS 25.0%
!! AACATTTAAAGTGTCCAATA+AGG - chr8_3:54077448-54077467 Msa1295860:CDS 25.0%
! CACATACATAATTTCCTTAT+TGG + chr8_3:54077465-54077484 None:intergenic 25.0%
! TATGTATGTGTAACATTGAA+TGG - chr8_3:54077475-54077494 Msa1295860:CDS 25.0%
!! TTAAATGTGTCTCATCTTTT+AGG + chr8_3:54077519-54077538 None:intergenic 25.0%
! AATTATACTTCGATTCACAA+AGG + chr8_3:54077563-54077582 None:intergenic 25.0%
! TGATAATGAGAATGCTGTTA+AGG - chr8_3:54075226-54075245 Msa1295860:three_prime_UTR 30.0%
!! GTTTTGTTAAGGAGCTAAAT+CGG - chr8_3:54075584-54075603 Msa1295860:intron 30.0%
! ACTCACAAGAATACAGATTT+TGG - chr8_3:54075627-54075646 Msa1295860:intron 30.0%
ATAGCAGGTTAAATATTGTC+GGG + chr8_3:54075723-54075742 None:intergenic 30.0%
! TGTCCTTTTCTTCATCAAAA+AGG + chr8_3:54075822-54075841 None:intergenic 30.0%
GATGAAGAAAAGGACATATT+TGG - chr8_3:54075826-54075845 Msa1295860:intron 30.0%
TGTAAATGGTCGAATCATAT+TGG - chr8_3:54075879-54075898 Msa1295860:intron 30.0%
TAACATATTCAGAACAGCAT+GGG + chr8_3:54075949-54075968 None:intergenic 30.0%
TTAACATATTCAGAACAGCA+TGG + chr8_3:54075950-54075969 None:intergenic 30.0%
!! TTTCTTCCTTTTTACTGACT+TGG - chr8_3:54076508-54076527 Msa1295860:intron 30.0%
AGTAGAATCAATGACATATC+GGG + chr8_3:54076630-54076649 None:intergenic 30.0%
!! GTCATTGATTCTACTACATA+TGG - chr8_3:54076635-54076654 Msa1295860:intron 30.0%
!! TTTTAATTGGCAACATCGTT+AGG - chr8_3:54076736-54076755 Msa1295860:intron 30.0%
TCAAAACCATCTTATGTTCA+AGG + chr8_3:54076777-54076796 None:intergenic 30.0%
AACTCTGTAATCACAAGATT+GGG + chr8_3:54077049-54077068 None:intergenic 30.0%
TAACTCTGTAATCACAAGAT+TGG + chr8_3:54077050-54077069 None:intergenic 30.0%
TGATTACAGAGTTATTGTAG+AGG - chr8_3:54077056-54077075 Msa1295860:intron 30.0%
GATTACAGAGTTATTGTAGA+GGG - chr8_3:54077057-54077076 Msa1295860:intron 30.0%
GATTGTAAGCATACACATAT+CGG - chr8_3:54077084-54077103 Msa1295860:intron 30.0%
! GCAGTAACTTGTAACAATAA+TGG + chr8_3:54077185-54077204 None:intergenic 30.0%
! AAATGTTGAGGTTCTAGTAA+AGG + chr8_3:54077365-54077384 None:intergenic 30.0%
ACAACAACAGAAAAATGTTG+AGG + chr8_3:54077377-54077396 None:intergenic 30.0%
GAGATGTTCTTTGCAAAGAA+AGG + chr8_3:54075155-54075174 None:intergenic 35.0%
TTCTTTGCAAAGAACATCTC+AGG - chr8_3:54075155-54075174 Msa1295860:three_prime_UTR 35.0%
AGATCTACTACACATATCAC+AGG + chr8_3:54075320-54075339 None:intergenic 35.0%
ATGTATATCTTCCTAAGCCT+TGG - chr8_3:54075375-54075394 Msa1295860:three_prime_UTR 35.0%
TGTATATCTTCCTAAGCCTT+GGG - chr8_3:54075376-54075395 Msa1295860:three_prime_UTR 35.0%
CTACGCAATTGATGAAGAAT+CGG + chr8_3:54075425-54075444 None:intergenic 35.0%
GAAAAATCACTTCATCCTTG+TGG - chr8_3:54075690-54075709 Msa1295860:CDS 35.0%
!!! TAACCTGCTATTTTGTTAGC+AGG - chr8_3:54075732-54075751 Msa1295860:intron 35.0%
AAATGGTCGAATCATATTGG+AGG - chr8_3:54075882-54075901 Msa1295860:intron 35.0%
TGTAGAGAAATTTCCAGCAT+TGG + chr8_3:54076130-54076149 None:intergenic 35.0%
TTCTCTACAAAACTTCAGGA+AGG - chr8_3:54076141-54076160 Msa1295860:CDS 35.0%
CCAAACAGAATATTGCAGAA+CGG - chr8_3:54076213-54076232 Msa1295860:CDS 35.0%
GGTCTTTATGACAGAGAAAA+AGG - chr8_3:54076254-54076273 Msa1295860:intron 35.0%
CTTTATGACAGAGAAAAAGG+TGG - chr8_3:54076257-54076276 Msa1295860:intron 35.0%
!! ACGTTTGTTATCTGATTCAC+AGG - chr8_3:54076335-54076354 Msa1295860:CDS 35.0%
!! CGTTTGTTATCTGATTCACA+GGG - chr8_3:54076336-54076355 Msa1295860:CDS 35.0%
TTATCTGATTCACAGGGATT+CGG - chr8_3:54076342-54076361 Msa1295860:CDS 35.0%
CAAAAGAAGAGATTACGTCT+TGG + chr8_3:54076477-54076496 None:intergenic 35.0%
CTTCGATCAATCTACTCATA+TGG + chr8_3:54076601-54076620 None:intergenic 35.0%
GTAGAATCAATGACATATCG+GGG + chr8_3:54076629-54076648 None:intergenic 35.0%
TGTCTAGGTTCATCAATAAC+AGG - chr8_3:54076663-54076682 Msa1295860:intron 35.0%
TAAGTGATGTGTGCATATAG+AGG + chr8_3:54077142-54077161 None:intergenic 35.0%
TTCAAAGATCCACAAAGACA+AGG + chr8_3:54077330-54077349 None:intergenic 35.0%
TTAGACTGTCCAGTATATTG+CGG - chr8_3:54077405-54077424 Msa1295860:CDS 35.0%
CAACTTATTCCGCAATATAC+TGG + chr8_3:54077417-54077436 None:intergenic 35.0%
ACACATTTAACATCTCGTTG+AGG - chr8_3:54077529-54077548 Msa1295860:CDS 35.0%
ATGCAATGACCTAAGCTTTG+CGG - chr8_3:54075283-54075302 Msa1295860:three_prime_UTR 40.0%
ACATAGGTAAACATCTCCCA+AGG + chr8_3:54075395-54075414 None:intergenic 40.0%
! AAGAATCGGCTTTGTCACAT+AGG + chr8_3:54075411-54075430 None:intergenic 40.0%
! ATTGCGTAGTCTTGATTCTG+AGG - chr8_3:54075436-54075455 Msa1295860:CDS 40.0%
TTGTCGGGAAAAAAACCACA+AGG + chr8_3:54075708-54075727 None:intergenic 40.0%
GATCCTGCTAACAAAATAGC+AGG + chr8_3:54075738-54075757 None:intergenic 40.0%
TATACGTGATCACACGTTTC+AGG + chr8_3:54075799-54075818 None:intergenic 40.0%
GGACATATTTGGGAAACCAA+TGG - chr8_3:54075837-54075856 Msa1295860:intron 40.0%
ATATTTGGGAAACCAATGGC+CGG - chr8_3:54075841-54075860 Msa1295860:intron 40.0%
AGGTTCGCATACGATGTAAA+TGG - chr8_3:54075865-54075884 Msa1295860:intron 40.0%
! ATTCAGAACAGCATGGGTTA+GGG + chr8_3:54075943-54075962 None:intergenic 40.0%
TATTCAGAACAGCATGGGTT+AGG + chr8_3:54075944-54075963 None:intergenic 40.0%
TTCGAGTGTAACGATAAGTG+CGG - chr8_3:54076174-54076193 Msa1295860:CDS 40.0%
CTGCAATATTCTGTTTGGAC+AGG + chr8_3:54076211-54076230 None:intergenic 40.0%
CCGTTCTGCAATATTCTGTT+TGG + chr8_3:54076216-54076235 None:intergenic 40.0%
GAACGGAGTTCGTGTTAAAT+TGG - chr8_3:54076230-54076249 Msa1295860:intron 40.0%
ACGTTTGTGTGTGAGTACAT+TGG - chr8_3:54076396-54076415 Msa1295860:CDS 40.0%
AACAGGCCAAGTCAGTAAAA+AGG + chr8_3:54076517-54076536 None:intergenic 40.0%
CTACATATGGCAATGTGTCT+AGG - chr8_3:54076648-54076667 Msa1295860:intron 40.0%
GTGAACAGTTGACATCAGTA+CGG - chr8_3:54077211-54077230 Msa1295860:intron 40.0%
GAAGGATATCCTTGTCTTTG+TGG - chr8_3:54077318-54077337 Msa1295860:intron 40.0%
!! TGTGGATCTTTGAAATGCTG+AGG - chr8_3:54077336-54077355 Msa1295860:CDS 40.0%
AAAATTAGGGTCGTCGCGAA+AGG - chr8_3:54075250-54075269 Msa1295860:three_prime_UTR 45.0%
TTGATTCTGCCGCAAAGCTT+AGG + chr8_3:54075295-54075314 None:intergenic 45.0%
GTAAACATCTCCCAAGGCTT+AGG + chr8_3:54075389-54075408 None:intergenic 45.0%
TACATCGTATGCGAACCTTC+CGG + chr8_3:54075863-54075882 None:intergenic 45.0%
CGGAGTTCGTGTTAAATTGG+AGG - chr8_3:54076233-54076252 Msa1295860:intron 45.0%
ATTCACAGGGATTCGGAGTA+AGG - chr8_3:54076349-54076368 Msa1295860:CDS 45.0%
TTCACAGGGATTCGGAGTAA+GGG - chr8_3:54076350-54076369 Msa1295860:CDS 45.0%
!!! TGGTGAAGTTTTAGAGCAGC+AGG - chr8_3:54076416-54076435 Msa1295860:intron 45.0%
!!! TGAAGTTTTAGAGCAGCAGG+AGG - chr8_3:54076419-54076438 Msa1295860:intron 45.0%
CAGAGTTATTGTAGAGGGCA+AGG - chr8_3:54077062-54077081 Msa1295860:intron 45.0%
GGCGAAATGCTCAAGCTTTC+TGG - chr8_3:54075176-54075195 Msa1295860:three_prime_UTR 50.0%
TATGAGCCTGTGCTCGTAGA+AGG - chr8_3:54077300-54077319 Msa1295860:intron 50.0%
GGATATCCTTCTACGAGCAC+AGG + chr8_3:54077309-54077328 None:intergenic 50.0%
TTGGGAAACCAATGGCCGGA+AGG - chr8_3:54075845-54075864 Msa1295860:intron 55.0%
TATGCGAACCTTCCGGCCAT+TGG + chr8_3:54075856-54075875 None:intergenic 55.0%
!! GCTGGCGAAGCTATTTTGCG+TGG - chr8_3:54076372-54076391 Msa1295860:CDS 55.0%
CAGGGATTCGGAGTAAGGGC+TGG - chr8_3:54076354-54076373 Msa1295860:CDS 60.0%
! CAGGAGGCACACAACAGACG+CGG - chr8_3:54076435-54076454 Msa1295860:intron 60.0%
AGGCACACAACAGACGCGGA+AGG - chr8_3:54076439-54076458 Msa1295860:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 54075139 54077650 54075139 ID=Msa1295860;Name=Msa1295860
chr8_3 mRNA 54075139 54077650 54075139 ID=Msa1295860-mRNA-1;Parent=Msa1295860;Name=Msa1295860-mRNA-1;_AED=0.12;_eAED=0.12;_QI=43|0.66|0.71|1|1|1|7|294|303
chr8_3 exon 54075139 54075525 54075139 ID=Msa1295860-mRNA-1:exon:15605;Parent=Msa1295860-mRNA-1
chr8_3 exon 54075664 54075724 54075664 ID=Msa1295860-mRNA-1:exon:15604;Parent=Msa1295860-mRNA-1
chr8_3 exon 54076105 54076236 54076105 ID=Msa1295860-mRNA-1:exon:15603;Parent=Msa1295860-mRNA-1
chr8_3 exon 54076329 54076432 54076329 ID=Msa1295860-mRNA-1:exon:15602;Parent=Msa1295860-mRNA-1
chr8_3 exon 54076535 54076630 54076535 ID=Msa1295860-mRNA-1:exon:15601;Parent=Msa1295860-mRNA-1
chr8_3 exon 54076886 54077035 54076886 ID=Msa1295860-mRNA-1:exon:15600;Parent=Msa1295860-mRNA-1
chr8_3 exon 54077332 54077650 54077332 ID=Msa1295860-mRNA-1:exon:15599;Parent=Msa1295860-mRNA-1
chr8_3 five_prime_UTR 54077608 54077650 54077608 ID=Msa1295860-mRNA-1:five_prime_utr;Parent=Msa1295860-mRNA-1
chr8_3 CDS 54077332 54077607 54077332 ID=Msa1295860-mRNA-1:cds;Parent=Msa1295860-mRNA-1
chr8_3 CDS 54076886 54077035 54076886 ID=Msa1295860-mRNA-1:cds;Parent=Msa1295860-mRNA-1
chr8_3 CDS 54076535 54076630 54076535 ID=Msa1295860-mRNA-1:cds;Parent=Msa1295860-mRNA-1
chr8_3 CDS 54076329 54076432 54076329 ID=Msa1295860-mRNA-1:cds;Parent=Msa1295860-mRNA-1
chr8_3 CDS 54076105 54076236 54076105 ID=Msa1295860-mRNA-1:cds;Parent=Msa1295860-mRNA-1
chr8_3 CDS 54075664 54075724 54075664 ID=Msa1295860-mRNA-1:cds;Parent=Msa1295860-mRNA-1
chr8_3 CDS 54075433 54075525 54075433 ID=Msa1295860-mRNA-1:cds;Parent=Msa1295860-mRNA-1
chr8_3 three_prime_UTR 54075139 54075432 54075139 ID=Msa1295860-mRNA-1:three_prime_utr;Parent=Msa1295860-mRNA-1
Gene Sequence

>Msa1295860

ATGCTCAAGCTTTCTGGTAATGATCATAATGAAGACTCTTGTATTGATAATGAGAATGCTGTTAAGGAAAAATTAGGGTCGTCGCGAAAGGTTATAGTGTTATGCAATGACCTAAGCTTTGCGGCAGAATCAACTCCTGTGATATGTGTAGTAGATCTACAAGTACTAAATTATCTTTGTGAGCAAGAGAGATATGTATATCTTCCTAAGCCTTGGGAGATGTTTACCTATGTGACAAAGCCGATTCTTCATCAATTGCGTAGTCTTGATTCTGAGGATCTGCAACTGTCTTGTAATTGCTTATATTCTACTTGCTGTCCTGAAACGTGTGATCACGTATACCTTTTTGATGAAGAAAAGGACATATTTGGGAAACCAATGGCCGGAAGGTTCGCATACGATGTAAATGGTCGAATCATATTGGAGGAAGGTAGTTTTGTCTTCGAGTGTAACGATAAGTGCGGATGCAATAAAACCTGTCCAAACAGAATATTGCAGAACGGAGTTCGTGTTAAATTGGAGGGATTCGGAGTAAGGGCTGGCGAAGCTATTTTGCGTGGCACGTTTGTGTGTGAGTACATTGGTGAAGTTTTAGAGCAGCAGGAGGCACACAACAGACGCGGAAGCAAAGAAAATTGCAGTTATTTCTTAGACATTGATGCTCGTGCTAACCATATGAGTAGATTGATCGAAGCACATCCCCGATATGTCATTGATTCTACTACATATGGCAATGTGTCTAGGTTCATCAATAACAGAGTTATTGTAGAGGGCAAGGATTGTAAGCATACACATATCGGTCTTTACGCAAGTCGAGATATTGCTTTGCATGAAGAATTGACATATAATTATGACTATGAGCCTGTGCTCGTAGAAGGATATCCTTGTCTTTGTGGATCTTTGAAATGCTGA
Protein sequence

>Msa1295860

MLKLSGNDHNEDSCIDNENAVKEKLGSSRKVIVLCNDLSFAAESTPVICVVDLQVLNYLCEQERYVYLPKPWEMFTYVTKPILHQLRSLDSEDLQLSCNCLYSTCCPETCDHVYLFDEEKDIFGKPMAGRFAYDVNGRIILEEGSFVFECNDKCGCNKTCPNRILQNGVRVKLEGFGVRAGEAILRGTFVCEYIGEVLEQQEAHNRRGSKENCSYFLDIDARANHMSRLIEAHPRYVIDSTTYGNVSRFINNRVIVEGKDCKHTHIGLYASRDIALHEELTYNYDYEPVLVEGYPCLCGSLKC*