AlfalfaGEDB | Msa1296030

M. sativa cultivar zhongmu-4 / Msa1296030

KEGG: K14498
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009987 GO:0010033 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1296030	sp\|Q39193\|SRK2I_ARATH	82.597	362	62	1	1	362	1	361	0.0	613

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1296030	S4TWB4	100.000	363	0	0	1	363	1	363	0.0	756

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1296030	MtrunA17_Chr8g0374571	99.449	363	2	0	1	363	1	363	0.0	754
Msa1296030	MtrunA17_Chr4g0045371	81.543	363	66	1	1	362	1	363	0.0	625
Msa1296030	MtrunA17_Chr5g0402661	83.754	357	56	2	6	362	1	355	0.0	603
Msa1296030	MtrunA17_Chr1g0162301	81.370	365	65	3	1	362	1	365	0.0	594
Msa1296030	MtrunA17_Chr4g0055451	67.941	340	97	2	21	357	3	333	1.42e-176	493
Msa1296030	MtrunA17_Chr8g0381821	69.298	342	101	2	21	362	2	339	6.10e-173	484
Msa1296030	MtrunA17_Chr2g0277501	70.938	320	92	1	21	339	2	321	6.58e-172	482
Msa1296030	MtrunA17_Chr3g0131061	74.086	301	77	1	21	320	2	302	2.08e-171	481
Msa1296030	MtrunA17_Chr8g0385281	73.754	301	78	1	21	320	3	303	2.24e-171	481
Msa1296030	MtrunA17_Chr5g0426521	68.182	330	98	2	21	345	2	329	7.74e-170	476

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1296030	AT5G66880.1	82.597	362	62	1	1	362	1	361	0.0	613
Msa1296030	AT5G66880.2	82.597	362	62	1	1	362	1	361	0.0	613
Msa1296030	AT3G50500.1	81.215	362	68	0	1	362	1	362	0.0	601
Msa1296030	AT3G50500.2	79.675	369	68	1	1	362	1	369	0.0	594
Msa1296030	AT4G33950.1	79.945	364	69	3	1	362	1	362	0.0	591
Msa1296030	AT4G33950.2	80.645	341	64	2	1	341	1	339	0.0	581
Msa1296030	AT5G63650.1	71.704	311	87	1	21	330	2	312	1.91e-170	479
Msa1296030	AT5G08590.1	71.336	307	88	0	21	327	2	308	2.77e-170	479
Msa1296030	AT1G10940.1	73.179	302	78	2	21	320	2	302	9.61e-170	478
Msa1296030	AT1G60940.1	71.383	311	88	1	21	330	2	312	2.17e-169	476

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Find 129 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATATTTGGATGGATT+GGG	0.385680	8_3:+54736502	None:intergenic
AAACAAAGATCCTTGAGATA+AGG	0.518593	8_3:+54732935	None:intergenic
AAACATGTACACATGAACTA+CGG	0.612439	8_3:+54731991	None:intergenic
AAAGAACAGGTTCCAGCTGC+TGG	0.474162	8_3:+54732582	None:intergenic
AAAGTTTAATTACCATTGCA+TGG	0.533259	8_3:+54735087	None:intergenic
AACCTTATATGTGATGCTAG+TGG	0.585559	8_3:-54733162	Msa1296030:CDS
AAGGAGAGCATTCTTAATAT+TGG	0.197222	8_3:-54732410	Msa1296030:three_prime_UTR
AATATCAGATTGCAGATGTT+TGG	0.368115	8_3:-54733196	Msa1296030:intron
ACATGTTGTATTTCAAGTAC+TGG	0.423910	8_3:+54732436	None:intergenic
ACCTTATATGTGATGCTAGT+GGG	0.561501	8_3:-54733161	Msa1296030:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTATTTACGAATTGAAGTC+GGG	-	chr8_3:54732765-54732784	Msa1296030:intron	30.0%
GCAAATTTACAAAACTGCAA+AGG	+	chr8_3:54732809-54732828	None:intergenic	30.0%
ACAAAAAATACACCTAAACC+AGG	+	chr8_3:54732857-54732876	None:intergenic	30.0%
TAACCTTATGTTATGACTGA+CGG	-	chr8_3:54732945-54732964	Msa1296030:CDS	30.0%
TCCTAACATTGTTAGGTTTA+AGG	-	chr8_3:54733059-54733078	Msa1296030:intron	30.0%
TCCTTAAACCTAACAATGTT+AGG	+	chr8_3:54733063-54733082	None:intergenic	30.0%
TAACATTGTTAGGTTTAAGG+AGG	-	chr8_3:54733062-54733081	Msa1296030:intron	30.0%
ATTACAATAGCAAGATGAGT+AGG	+	chr8_3:54733180-54733199	None:intergenic	30.0%
TTCACACTCTATGAATAAAC+AGG	+	chr8_3:54733301-54733320	None:intergenic	30.0%
TTCTTTCAACAACTCATATC+TGG	-	chr8_3:54733385-54733404	Msa1296030:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	54731956	54736573	54731956	ID=Msa1296030;Name=Msa1296030
chr8_3	mRNA	54731956	54736573	54731956	ID=Msa1296030-mRNA-1;Parent=Msa1296030;Name=Msa1296030-mRNA-1;_AED=0.10;_eAED=0.10;_QI=215\|1\|1\|1\|1\|1\|9\|514\|363
chr8_3	exon	54731956	54732763	54731956	ID=Msa1296030-mRNA-1:exon:15712;Parent=Msa1296030-mRNA-1
chr8_3	exon	54732921	54733019	54732921	ID=Msa1296030-mRNA-1:exon:15711;Parent=Msa1296030-mRNA-1
chr8_3	exon	54733106	54733210	54733106	ID=Msa1296030-mRNA-1:exon:15710;Parent=Msa1296030-mRNA-1
chr8_3	exon	54734111	54734203	54734111	ID=Msa1296030-mRNA-1:exon:15709;Parent=Msa1296030-mRNA-1
chr8_3	exon	54734861	54734953	54734861	ID=Msa1296030-mRNA-1:exon:15708;Parent=Msa1296030-mRNA-1
chr8_3	exon	54735100	54735153	54735100	ID=Msa1296030-mRNA-1:exon:15707;Parent=Msa1296030-mRNA-1
chr8_3	exon	54735263	54735364	54735263	ID=Msa1296030-mRNA-1:exon:15706;Parent=Msa1296030-mRNA-1
chr8_3	exon	54735446	54735520	54735446	ID=Msa1296030-mRNA-1:exon:15705;Parent=Msa1296030-mRNA-1
chr8_3	exon	54736182	54736573	54736182	ID=Msa1296030-mRNA-1:exon:15704;Parent=Msa1296030-mRNA-1
chr8_3	five_prime_UTR	54736359	54736573	54736359	ID=Msa1296030-mRNA-1:five_prime_utr;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54736182	54736358	54736182	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54735446	54735520	54735446	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54735263	54735364	54735263	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54735100	54735153	54735100	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54734861	54734953	54734861	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54734111	54734203	54734111	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54733106	54733210	54733106	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54732921	54733019	54732921	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	CDS	54732470	54732763	54732470	ID=Msa1296030-mRNA-1:cds;Parent=Msa1296030-mRNA-1
chr8_3	three_prime_UTR	54731956	54732469	54731956	ID=Msa1296030-mRNA-1:three_prime_utr;Parent=Msa1296030-mRNA-1

Gene Sequence

>Msa1296030

ATGGATCGAGCTGCGATGACGGTCGGACCGGGTATGGATTTACCGATCATGCACGACAGTGATCGGTACGATCTGGTTCGTGATATCGGGTCCGGAAATTTCGGTATTGCTAGGTTGATGCAAGATAAACAAACCAAAGAGCTTGTTGCTGTTAAATATATCGAACGTGGTGATAAGATTGATGAAAATGTGAAGAGAGAAATTATTAATCACAGGTCTCTGAGACATCCTAACATTGTTAGGTTTAAGGAGGTCATTTTAACACCTACTCATCTTGCTATTGTAATGGAGTATGCATCTGGAGGAGAACTTTTCGAGCGAATCAGTAATGCTGGCCATTTTTCTGAGGATGAGGCTCGTTTCTTCTTTCAACAACTCATATCTGGGGTCAGCTACTGCCATGCAATGCAAGTATGTCACCGGGACCTGAAGTTGGAAAATACTTTGTTGGATGGAAGCCCAACTCCTCGTTTGAAAATATGTGATTTTGGTTACTCCAAGTCTTCAGTGCTTCATTCACAACCGAAGTCAACTGTGGGAACTCCGGCATATATTGCTCCAGAAGTATTACTGAGACAAGAGTATGATGGAAAGATTGCAGATGTTTGGTCTTGTGGGGTAACCTTATATGTGATGCTAGTGGGATCGTATCCCTTTGAAGATCCTAACGAGCCAAAAGATTTCCGGAAGACAATACAGAGAGTACTCAGTGTCCAGTATTCCATTCCAGACAATGTTCAGATAACTCCAGAGTGTCGCCACCTTATCTCAAGGATCTTTGTTTTTGACCCTGCAGAGAGAATTACCATGCCCGAAATCTGGAAACATAAATGGTTTCTGAAGAATCTTCCCATGGACCTGATGGATGAGAAGATAATGGGTAACCAATTTGAAGAGCCTGAACAACCCATGCAGAGCATTGATGCAATCATGCAGATAATTTCAGAAGCTACTATACCAGCAGCTGGAACCTGTTCTTTAGACCAGTTTATGGCAGATAACATTGATATGGACGATGAATTTGATGACTTGGAATATGAATCCGAGCTTGATATAGATAGCAGTGGGGAGATAGTGTATGCTATGTACTAA

Protein sequence

>Msa1296030

MDRAAMTVGPGMDLPIMHDSDRYDLVRDIGSGNFGIARLMQDKQTKELVAVKYIERGDKIDENVKREIINHRSLRHPNIVRFKEVILTPTHLAIVMEYASGGELFERISNAGHFSEDEARFFFQQLISGVSYCHAMQVCHRDLKLENTLLDGSPTPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLLRQEYDGKIADVWSCGVTLYVMLVGSYPFEDPNEPKDFRKTIQRVLSVQYSIPDNVQITPECRHLISRIFVFDPAERITMPEIWKHKWFLKNLPMDLMDEKIMGNQFEEPEQPMQSIDAIMQIISEATIPAAGTCSLDQFMADNIDMDDEFDDLEYESELDIDSSGEIVYAMY*