AlfalfaGEDB | Msa1297930

M. sativa cultivar zhongmu-4 / Msa1297930

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297930	XP_013457554.1	96.936	359	11	0	1	359	1	359	0.0	712

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297930	A0A072V0D3	96.936	359	11	0	1	359	1	359	0.0	712

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TFs/TRs:

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Gene ID	Type	Classification
Msa1297930	TF	SRS

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297930	MtrunA17_Chr4g0055241	96.936	359	11	0	1	359	1	359	0.0	712

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297930	AT1G32730.1	45.533	347	157	7	18	359	8	327	5.05e-90	273

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Find 94 sgRNAs with CRISPR-Local

Find 573 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTAGAGTTAGGCAAAA+CGG	0.513095	8_3:-58583923	Msa1297930:three_prime_UTR
AAACCTAAGTGCAAACAGTG+TGG	0.715094	8_3:-58591369	Msa1297930:CDS
AAAGAAGCCACTAACTAGAA+AGG	0.540503	8_3:-58584808	Msa1297930:intron
AACAATGAGGTAATGATCCC+TGG	0.455916	8_3:-58584499	Msa1297930:CDS
AACAATTCAACGGCGAAAGT+CGG	0.518604	8_3:-58591456	Msa1297930:CDS
AACTGACCATGAATCGGACA+AGG	0.662088	8_3:+58591200	None:intergenic
AAGCTCAAGTCTGCTGTAGA+TGG	0.465375	8_3:-58584391	Msa1297930:CDS
AATCTTCGAATAAGAGAAAG+AGG	0.496068	8_3:-58584333	Msa1297930:CDS
AATTAACAAAGCCCGAAGTG+AGG	0.646396	8_3:-58584272	Msa1297930:CDS
ACAATGAGGTAATGATCCCT+GGG	0.596258	8_3:-58584498	Msa1297930:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTGAAGGAAAAATGGGT+GGG	+	chr8_3:58583579-58583598	None:intergenic	30.0%
AAATTTGAAGGAAAAATGGG+TGG	+	chr8_3:58583580-58583599	None:intergenic	30.0%
TTGGGAAGAGAAAAATTTGA+AGG	+	chr8_3:58583592-58583611	None:intergenic	30.0%
TTGTTCTTTATGCGTGTTTA+TGG	-	chr8_3:58583736-58583755	Msa1297930:three_prime_UTR	30.0%
TGTTCAAACATCGAGTTAAA+CGG	+	chr8_3:58584410-58584429	None:intergenic	30.0%
ATTATTACGAAACTGAAGCT+AGG	-	chr8_3:58584537-58584556	Msa1297930:CDS	30.0%
TTATTACGAAACTGAAGCTA+GGG	-	chr8_3:58584538-58584557	Msa1297930:CDS	30.0%
TAACGTGAAAACATGAAATC+AGG	-	chr8_3:58584755-58584774	Msa1297930:intron	30.0%
TTGGTAGGATCTTTCAAATT+TGG	-	chr8_3:58584944-58584963	Msa1297930:intron	30.0%
ATATTGTATCAGATCGAAAG+GGG	+	chr8_3:58585078-58585097	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	58583495	58591756	58583495	ID=Msa1297930;Name=Msa1297930
chr8_3	mRNA	58583495	58591756	58583495	ID=Msa1297930-mRNA-1;Parent=Msa1297930;Name=Msa1297930-mRNA-1;_AED=0.02;_eAED=0.02;_QI=260\|1\|1\|1\|0.75\|1\|5\|535\|359
chr8_3	exon	58583495	58584710	58583495	ID=Msa1297930-mRNA-1:exon:16830;Parent=Msa1297930-mRNA-1
chr8_3	exon	58584809	58584911	58584809	ID=Msa1297930-mRNA-1:exon:16829;Parent=Msa1297930-mRNA-1
chr8_3	exon	58589551	58589638	58589551	ID=Msa1297930-mRNA-1:exon:16828;Parent=Msa1297930-mRNA-1
chr8_3	exon	58591207	58591268	58591207	ID=Msa1297930-mRNA-1:exon:16827;Parent=Msa1297930-mRNA-1
chr8_3	exon	58591351	58591756	58591351	ID=Msa1297930-mRNA-1:exon:16826;Parent=Msa1297930-mRNA-1
chr8_3	five_prime_UTR	58591497	58591756	58591497	ID=Msa1297930-mRNA-1:five_prime_utr;Parent=Msa1297930-mRNA-1
chr8_3	CDS	58591351	58591496	58591351	ID=Msa1297930-mRNA-1:cds;Parent=Msa1297930-mRNA-1
chr8_3	CDS	58591207	58591268	58591207	ID=Msa1297930-mRNA-1:cds;Parent=Msa1297930-mRNA-1
chr8_3	CDS	58589551	58589638	58589551	ID=Msa1297930-mRNA-1:cds;Parent=Msa1297930-mRNA-1
chr8_3	CDS	58584809	58584911	58584809	ID=Msa1297930-mRNA-1:cds;Parent=Msa1297930-mRNA-1
chr8_3	CDS	58584030	58584710	58584030	ID=Msa1297930-mRNA-1:cds;Parent=Msa1297930-mRNA-1
chr8_3	three_prime_UTR	58583495	58584029	58583495	ID=Msa1297930-mRNA-1:three_prime_utr;Parent=Msa1297930-mRNA-1

Gene Sequence

>Msa1297930

ATGGCGACGGCACCGAGCAACAATTCAACGGCGAAAGTCGGAACCCTAGGTCTAACCAATTCACCGGTGGCTTCATCGGCGCCGAAATCTCAACGCGGTCAGAACAAACCTAAGTGCAAACAGTGTGGCAATGTTGCTCGTTCTAGGTGTCCTTATGAGCAATGCAAGAGTTGCTGTTCCAAGAATCAAAATCCTTGTCCGATTCATGTGTTAAAAGCCAGTACAATTCCTGACAACACTCCATCTTCTGGTGCTGCCCCACTTGATCGAAAATCCTTTGAACCACCCCCCTCAATAAGTGCTGGTAGAGCTGCGTCACTTCGACAACTTTCCAATAGTTTTGCTCAGTTCAATAATTTGCATCTTTCACTTCGATCAAAGAAGCCACTAACTAGAAAGGATGCTGCAGCTATCAATGAATGGAGGTTTTCCAAGTTAAAGGAATACAAGGAGCGAAATGTTGAAGCGGAAAATGAAGCCTTTGACCGATACATGCAAAATGTAGATTTGCTGGAAGAAGTATTATCTGTGAAGCATTCAGATGAGGATGTGTCTTCTGCATCGGAGGCTAATCCTACCTCAACAGAGAACAATGAGGTAATGATCCCTGGGTTAAAATTGCAACTAAGGTCAAACTCGACAAGAAGTGATGGTGTTAGAATAAGAATAAAAAAAACTGTAGATGATGGATTGAAGAAGCTCAAGTCTGCTGTAGATGGTGACAGCAATAATGAACAAATCGATGAAGTTAGTGAATCTTCGAATAAGAGAAAGAGGACCGAAAAGTTATCAGCTATAAGTGATCTTCTGGACAAAATTAACAAAGCCCGAAGTGAGGAGGATTTGCAGTCATGCTTGGAGGTGAAGTTACAGCTTTTCAATTCAGACGAAGACTCTGCTATGGTGCAACACACAGACAATGAAATGCATCAGAATCAAACTGCTGAAGGCGATGTTGTACCAACAAAAGAATTGGATTACTCGTTACCAAAATTGGTTGGGTCCTCTGAAATTGATCAAGAAACTCTCAACATCATTGACAAACACTTTTCTTCTCTCGAGAATGTAGAGCAGTTATGA

Protein sequence

>Msa1297930

MATAPSNNSTAKVGTLGLTNSPVASSAPKSQRGQNKPKCKQCGNVARSRCPYEQCKSCCSKNQNPCPIHVLKASTIPDNTPSSGAAPLDRKSFEPPPSISAGRAASLRQLSNSFAQFNNLHLSLRSKKPLTRKDAAAINEWRFSKLKEYKERNVEAENEAFDRYMQNVDLLEEVLSVKHSDEDVSSASEANPTSTENNEVMIPGLKLQLRSNSTRSDGVRIRIKKTVDDGLKKLKSAVDGDSNNEQIDEVSESSNKRKRTEKLSAISDLLDKINKARSEEDLQSCLEVKLQLFNSDEDSAMVQHTDNEMHQNQTAEGDVVPTKELDYSLPKLVGSSEIDQETLNIIDKHFSSLENVEQL*