AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1299060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299060 MtrunA17_Chr4g0059631 81.439 264 40 3 1 264 1 255 7.37e-156 434
Msa1299060 MtrunA17_Chr4g0022641 53.710 283 99 3 1 264 1 270 1.27e-97 291
Msa1299060 MtrunA17_Chr1g0212471 58.788 165 66 1 1 163 1 165 3.23e-61 191
Msa1299060 MtrunA17_Chr2g0303981 48.993 149 74 2 3 150 5 152 7.35e-44 147
Msa1299060 MtrunA17_Chr1g0212151 42.000 150 84 1 1 147 1 150 8.91e-35 127
Msa1299060 MtrunA17_Chr6g0457851 37.255 153 93 1 1 150 11 163 1.47e-34 127
Msa1299060 MtrunA17_Chr6g0449851 39.216 153 90 1 1 150 1 153 3.65e-34 125
Msa1299060 MtrunA17_Chr6g0449861 35.948 153 95 1 1 150 9 161 1.63e-28 111
Msa1299060 MtrunA17_Chr3g0107381 31.618 136 87 4 1 135 1 131 1.75e-14 70.9
Msa1299060 MtrunA17_Chr2g0283941 23.043 230 163 4 1 218 1 228 7.46e-14 69.3
Msa1299060 MtrunA17_Chr5g0432691 31.132 106 69 3 1 105 1 103 5.62e-11 59.7
Msa1299060 MtrunA17_Chr3g0107461 30.252 119 75 4 1 114 1 116 9.25e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299060 AT5G55690.1 32.000 175 103 4 1 166 1 168 2.87e-17 79.7
Msa1299060 AT5G55690.2 32.000 175 103 4 1 166 1 168 2.87e-17 79.7
Msa1299060 AT1G22590.2 35.849 106 64 3 1 105 1 103 5.41e-11 60.1

Find 44 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACACTTGTAAGGTCTTC+TGG 0.270047 8_3:-60050813 None:intergenic
AAGTTGTTAATCCTGATTAT+AGG 0.299671 8_3:+60051326 Msa1299060:CDS
TCCTGAGATATTTGATGTTA+AGG 0.348371 8_3:+60050878 Msa1299060:CDS
GCACTTTGGATGTATTTCAT+AGG 0.357011 8_3:-60050651 None:intergenic
ATCCCACTAAGTTGGTTTGA+TGG 0.398405 8_3:-60051175 None:intergenic
ACCATCTCACTTGTATAATC+AGG 0.418843 8_3:-60051226 None:intergenic
GATCAACAAGTTGACTAAGT+TGG 0.422061 8_3:-60051405 None:intergenic
GCAAGGAGTTTACACTTGTA+AGG 0.440354 8_3:-60050821 None:intergenic
ACAATCTCACTCCTATAATC+AGG 0.445065 8_3:-60051337 None:intergenic
TATAATCAGGATTAACTACT+TGG 0.446120 8_3:-60051213 None:intergenic
AGTTGGTTTGCTGATGAACT+TGG 0.448642 8_3:-60051388 None:intergenic
TCTCCACAATCCCACTAAGT+TGG 0.470552 8_3:-60051183 None:intergenic
CTAAAATGTGGATGCCAAGT+TGG 0.479466 8_3:-60050978 None:intergenic
TAAAATGTGGATGCCAAGTT+GGG 0.491000 8_3:-60050977 None:intergenic
GCTTGCTTTATTGTGTATGA+TGG 0.491871 8_3:+60050759 Msa1299060:CDS
TCATCATCAAACCAACTTAG+TGG 0.501564 8_3:+60051283 Msa1299060:CDS
TCCTTAACATCAAATATCTC+AGG 0.501707 8_3:-60050879 None:intergenic
CTTTGAAAATAAGATAGAAA+AGG 0.507369 8_3:+60050905 Msa1299060:CDS
TCTCCACAAGCCCACTAAGT+TGG 0.508925 8_3:-60051294 None:intergenic
TTGTAAGAAGTCTGGAGCAA+AGG 0.512867 8_3:+60050737 Msa1299060:CDS
AACTTGTTGATCTTGATGAC+TGG 0.515181 8_3:+60051416 Msa1299060:CDS
TGATTATAGGAGTGAGATTG+TGG 0.520372 8_3:+60051339 Msa1299060:CDS
ATTGTGGATTTCACTAGTCT+TGG 0.527589 8_3:+60051355 Msa1299060:CDS
TCACCATCAAACCAACTTAG+TGG 0.529150 8_3:+60051172 Msa1299060:CDS
AGTTGGTTTGATGATGAACT+AGG 0.530891 8_3:-60051277 None:intergenic
TATAATCAGGATTAACAACT+TGG 0.536057 8_3:-60051324 None:intergenic
AAACCAACTTAGTGGGCTTG+TGG 0.552156 8_3:+60051291 Msa1299060:CDS
CATCATCAAACCAACTTAGT+GGG 0.554520 8_3:+60051284 Msa1299060:CDS
AAACCAACTTAGTGGGATTG+TGG 0.556734 8_3:+60051180 Msa1299060:CDS
AGGTCTTCTGGCCATGTCGT+AGG 0.557005 8_3:-60050801 None:intergenic
CACCATCAAACCAACTTAGT+GGG 0.566836 8_3:+60051173 Msa1299060:CDS
TCATCCAAGTTACTAAAATG+TGG 0.567439 8_3:-60050990 None:intergenic
ATGGTGGATTTCACAAGTCT+TGG 0.570027 8_3:+60051244 Msa1299060:CDS
TGGTTTGCTGATGAACTTGG+AGG 0.570787 8_3:-60051385 None:intergenic
ACTTTCAAACAAAGAAAGCA+TGG 0.571291 8_3:+60050690 Msa1299060:CDS
TCACTAAGATCTACCCAACT+TGG 0.574367 8_3:+60050964 Msa1299060:CDS
ATTCTGATACTCTTGAAGCA+AGG 0.580425 8_3:-60050838 None:intergenic
TGATTATACAAGTGAGATGG+TGG 0.591946 8_3:+60051228 Msa1299060:CDS
TCCTGATTATACAAGTGAGA+TGG 0.603434 8_3:+60051225 Msa1299060:CDS
AAACAAAGATTAACAAAGTG+CGG 0.604618 8_3:+60050931 Msa1299060:CDS
TGGATAAATCAACTAGATGA+TGG 0.621105 8_3:+60051436 Msa1299060:CDS
TGGTTTGATGATGAACTAGG+AGG 0.662339 8_3:-60051274 None:intergenic
ATAATATTGAACCTACGACA+TGG 0.717032 8_3:+60050790 Msa1299060:CDS
AAGTTGGGTAGATCTTAGTG+AGG 0.717752 8_3:-60050962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGAAAATAAGATAGAAA+AGG + chr8_3:60050905-60050924 Msa1299060:CDS 20.0%
!!! CTTTGAAAATAAGATAGAAA+AGG + chr8_3:60050905-60050924 Msa1299060:CDS 20.0%
! AAACAAAGATTAACAAAGTG+CGG + chr8_3:60050931-60050950 Msa1299060:CDS 25.0%
!!! ATGATTGATTTTTCTGATCT+TGG + chr8_3:60051131-60051150 Msa1299060:intron 25.0%
! TATAATCAGGATTAACTACT+TGG - chr8_3:60051216-60051235 None:intergenic 25.0%
! TATAATCAGGATTAACAACT+TGG - chr8_3:60051327-60051346 None:intergenic 25.0%
! AAGTTGTTAATCCTGATTAT+AGG + chr8_3:60051326-60051345 Msa1299060:CDS 25.0%
! AAACAAAGATTAACAAAGTG+CGG + chr8_3:60050931-60050950 Msa1299060:CDS 25.0%
!!! ATGATTGATTTTTCTGATCT+TGG + chr8_3:60051131-60051150 Msa1299060:intron 25.0%
! TATAATCAGGATTAACTACT+TGG - chr8_3:60051216-60051235 None:intergenic 25.0%
! TATAATCAGGATTAACAACT+TGG - chr8_3:60051327-60051346 None:intergenic 25.0%
! AAGTTGTTAATCCTGATTAT+AGG + chr8_3:60051326-60051345 Msa1299060:CDS 25.0%
ACTTTCAAACAAAGAAAGCA+TGG + chr8_3:60050690-60050709 Msa1299060:CDS 30.0%
! TCTGAATTTTGTAAGAAGTC+TGG + chr8_3:60050729-60050748 Msa1299060:CDS 30.0%
ATAATATTGAACCTACGACA+TGG + chr8_3:60050790-60050809 Msa1299060:CDS 30.0%
TCCTGAGATATTTGATGTTA+AGG + chr8_3:60050878-60050897 Msa1299060:CDS 30.0%
TCCTTAACATCAAATATCTC+AGG - chr8_3:60050882-60050901 None:intergenic 30.0%
TCATCCAAGTTACTAAAATG+TGG - chr8_3:60050993-60051012 None:intergenic 30.0%
ACAAATAGAAACTAGCACTT+TGG + chr8_3:60051097-60051116 Msa1299060:intron 30.0%
TGGATAAATCAACTAGATGA+TGG + chr8_3:60051436-60051455 Msa1299060:CDS 30.0%
TCTTTCCAAAAAAACTCATC+AGG - chr8_3:60051484-60051503 None:intergenic 30.0%
ACTTTCAAACAAAGAAAGCA+TGG + chr8_3:60050690-60050709 Msa1299060:CDS 30.0%
! TCTGAATTTTGTAAGAAGTC+TGG + chr8_3:60050729-60050748 Msa1299060:CDS 30.0%
ATAATATTGAACCTACGACA+TGG + chr8_3:60050790-60050809 Msa1299060:CDS 30.0%
TCCTGAGATATTTGATGTTA+AGG + chr8_3:60050878-60050897 Msa1299060:CDS 30.0%
TCCTTAACATCAAATATCTC+AGG - chr8_3:60050882-60050901 None:intergenic 30.0%
TCATCCAAGTTACTAAAATG+TGG - chr8_3:60050993-60051012 None:intergenic 30.0%
ACAAATAGAAACTAGCACTT+TGG + chr8_3:60051097-60051116 Msa1299060:intron 30.0%
TGGATAAATCAACTAGATGA+TGG + chr8_3:60051436-60051455 Msa1299060:CDS 30.0%
TCTTTCCAAAAAAACTCATC+AGG - chr8_3:60051484-60051503 None:intergenic 30.0%
GCACTTTGGATGTATTTCAT+AGG - chr8_3:60050654-60050673 None:intergenic 35.0%
! GCTTGCTTTATTGTGTATGA+TGG + chr8_3:60050759-60050778 Msa1299060:CDS 35.0%
TTTACACTTGTAAGGTCTTC+TGG - chr8_3:60050816-60050835 None:intergenic 35.0%
ATTCTGATACTCTTGAAGCA+AGG - chr8_3:60050841-60050860 None:intergenic 35.0%
TAAAATGTGGATGCCAAGTT+GGG - chr8_3:60050980-60050999 None:intergenic 35.0%
!! GCATCCACATTTTAGTAACT+TGG + chr8_3:60050986-60051005 Msa1299060:CDS 35.0%
!! TTGGTTTGATGGTGAACTAT+AGG - chr8_3:60051167-60051186 None:intergenic 35.0%
TCCTGATTATACAAGTGAGA+TGG + chr8_3:60051225-60051244 Msa1299060:CDS 35.0%
ACCATCTCACTTGTATAATC+AGG - chr8_3:60051229-60051248 None:intergenic 35.0%
TGATTATACAAGTGAGATGG+TGG + chr8_3:60051228-60051247 Msa1299060:CDS 35.0%
!! AGTTGGTTTGATGATGAACT+AGG - chr8_3:60051280-60051299 None:intergenic 35.0%
TCATCATCAAACCAACTTAG+TGG + chr8_3:60051283-60051302 Msa1299060:CDS 35.0%
CATCATCAAACCAACTTAGT+GGG + chr8_3:60051284-60051303 Msa1299060:CDS 35.0%
ACAATCTCACTCCTATAATC+AGG - chr8_3:60051340-60051359 None:intergenic 35.0%
TGATTATAGGAGTGAGATTG+TGG + chr8_3:60051339-60051358 Msa1299060:CDS 35.0%
! ATTGTGGATTTCACTAGTCT+TGG + chr8_3:60051355-60051374 Msa1299060:CDS 35.0%
! GATCAACAAGTTGACTAAGT+TGG - chr8_3:60051408-60051427 None:intergenic 35.0%
!! AACTTGTTGATCTTGATGAC+TGG + chr8_3:60051416-60051435 Msa1299060:CDS 35.0%
!!! GTCAACCTGATGAGTTTTTT+TGG + chr8_3:60051476-60051495 Msa1299060:CDS 35.0%
GCACTTTGGATGTATTTCAT+AGG - chr8_3:60050654-60050673 None:intergenic 35.0%
! GCTTGCTTTATTGTGTATGA+TGG + chr8_3:60050759-60050778 Msa1299060:CDS 35.0%
TTTACACTTGTAAGGTCTTC+TGG - chr8_3:60050816-60050835 None:intergenic 35.0%
ATTCTGATACTCTTGAAGCA+AGG - chr8_3:60050841-60050860 None:intergenic 35.0%
TAAAATGTGGATGCCAAGTT+GGG - chr8_3:60050980-60050999 None:intergenic 35.0%
!! GCATCCACATTTTAGTAACT+TGG + chr8_3:60050986-60051005 Msa1299060:CDS 35.0%
!! TTGGTTTGATGGTGAACTAT+AGG - chr8_3:60051167-60051186 None:intergenic 35.0%
TCCTGATTATACAAGTGAGA+TGG + chr8_3:60051225-60051244 Msa1299060:CDS 35.0%
ACCATCTCACTTGTATAATC+AGG - chr8_3:60051229-60051248 None:intergenic 35.0%
TGATTATACAAGTGAGATGG+TGG + chr8_3:60051228-60051247 Msa1299060:CDS 35.0%
!! AGTTGGTTTGATGATGAACT+AGG - chr8_3:60051280-60051299 None:intergenic 35.0%
TCATCATCAAACCAACTTAG+TGG + chr8_3:60051283-60051302 Msa1299060:CDS 35.0%
CATCATCAAACCAACTTAGT+GGG + chr8_3:60051284-60051303 Msa1299060:CDS 35.0%
ACAATCTCACTCCTATAATC+AGG - chr8_3:60051340-60051359 None:intergenic 35.0%
TGATTATAGGAGTGAGATTG+TGG + chr8_3:60051339-60051358 Msa1299060:CDS 35.0%
! ATTGTGGATTTCACTAGTCT+TGG + chr8_3:60051355-60051374 Msa1299060:CDS 35.0%
! GATCAACAAGTTGACTAAGT+TGG - chr8_3:60051408-60051427 None:intergenic 35.0%
!! AACTTGTTGATCTTGATGAC+TGG + chr8_3:60051416-60051435 Msa1299060:CDS 35.0%
!!! GTCAACCTGATGAGTTTTTT+TGG + chr8_3:60051476-60051495 Msa1299060:CDS 35.0%
! TTTTGCGAGATTTCGCACTT+TGG - chr8_3:60050668-60050687 None:intergenic 40.0%
! TTGTAAGAAGTCTGGAGCAA+AGG + chr8_3:60050737-60050756 Msa1299060:CDS 40.0%
GCAAGGAGTTTACACTTGTA+AGG - chr8_3:60050824-60050843 None:intergenic 40.0%
AAGTTGGGTAGATCTTAGTG+AGG - chr8_3:60050965-60050984 None:intergenic 40.0%
TCACTAAGATCTACCCAACT+TGG + chr8_3:60050964-60050983 Msa1299060:CDS 40.0%
CTAAAATGTGGATGCCAAGT+TGG - chr8_3:60050981-60051000 None:intergenic 40.0%
!! GTTTGATGGTGAACTATAGG+AGG - chr8_3:60051164-60051183 None:intergenic 40.0%
TCACCATCAAACCAACTTAG+TGG + chr8_3:60051172-60051191 Msa1299060:CDS 40.0%
CACCATCAAACCAACTTAGT+GGG + chr8_3:60051173-60051192 Msa1299060:CDS 40.0%
!! ATCCCACTAAGTTGGTTTGA+TGG - chr8_3:60051178-60051197 None:intergenic 40.0%
AAACCAACTTAGTGGGATTG+TGG + chr8_3:60051180-60051199 Msa1299060:CDS 40.0%
!! ATGGTGGATTTCACAAGTCT+TGG + chr8_3:60051244-60051263 Msa1299060:CDS 40.0%
!! TGGTTTGATGATGAACTAGG+AGG - chr8_3:60051277-60051296 None:intergenic 40.0%
!! AGTTGGTTTGCTGATGAACT+TGG - chr8_3:60051391-60051410 None:intergenic 40.0%
!! TAGATGATGGTGTTTTGTGC+TGG + chr8_3:60051449-60051468 Msa1299060:CDS 40.0%
!! AGATGATGGTGTTTTGTGCT+GGG + chr8_3:60051450-60051469 Msa1299060:CDS 40.0%
! TTTTGCGAGATTTCGCACTT+TGG - chr8_3:60050668-60050687 None:intergenic 40.0%
! TTGTAAGAAGTCTGGAGCAA+AGG + chr8_3:60050737-60050756 Msa1299060:CDS 40.0%
GCAAGGAGTTTACACTTGTA+AGG - chr8_3:60050824-60050843 None:intergenic 40.0%
AAGTTGGGTAGATCTTAGTG+AGG - chr8_3:60050965-60050984 None:intergenic 40.0%
TCACTAAGATCTACCCAACT+TGG + chr8_3:60050964-60050983 Msa1299060:CDS 40.0%
CTAAAATGTGGATGCCAAGT+TGG - chr8_3:60050981-60051000 None:intergenic 40.0%
!! GTTTGATGGTGAACTATAGG+AGG - chr8_3:60051164-60051183 None:intergenic 40.0%
TCACCATCAAACCAACTTAG+TGG + chr8_3:60051172-60051191 Msa1299060:CDS 40.0%
CACCATCAAACCAACTTAGT+GGG + chr8_3:60051173-60051192 Msa1299060:CDS 40.0%
!! ATCCCACTAAGTTGGTTTGA+TGG - chr8_3:60051178-60051197 None:intergenic 40.0%
AAACCAACTTAGTGGGATTG+TGG + chr8_3:60051180-60051199 Msa1299060:CDS 40.0%
!! ATGGTGGATTTCACAAGTCT+TGG + chr8_3:60051244-60051263 Msa1299060:CDS 40.0%
!! TGGTTTGATGATGAACTAGG+AGG - chr8_3:60051277-60051296 None:intergenic 40.0%
!! AGTTGGTTTGCTGATGAACT+TGG - chr8_3:60051391-60051410 None:intergenic 40.0%
!! TAGATGATGGTGTTTTGTGC+TGG + chr8_3:60051449-60051468 Msa1299060:CDS 40.0%
!! AGATGATGGTGTTTTGTGCT+GGG + chr8_3:60051450-60051469 Msa1299060:CDS 40.0%
TCTCCACAATCCCACTAAGT+TGG - chr8_3:60051186-60051205 None:intergenic 45.0%
!! AAACCAACTTAGTGGGCTTG+TGG + chr8_3:60051291-60051310 Msa1299060:CDS 45.0%
!! TGGTTTGCTGATGAACTTGG+AGG - chr8_3:60051388-60051407 None:intergenic 45.0%
TCTCCACAATCCCACTAAGT+TGG - chr8_3:60051186-60051205 None:intergenic 45.0%
!! AAACCAACTTAGTGGGCTTG+TGG + chr8_3:60051291-60051310 Msa1299060:CDS 45.0%
!! TGGTTTGCTGATGAACTTGG+AGG - chr8_3:60051388-60051407 None:intergenic 45.0%
TCTCCACAAGCCCACTAAGT+TGG - chr8_3:60051297-60051316 None:intergenic 50.0%
TCTCCACAAGCCCACTAAGT+TGG - chr8_3:60051297-60051316 None:intergenic 50.0%
AGGTCTTCTGGCCATGTCGT+AGG - chr8_3:60050804-60050823 None:intergenic 55.0%
AGGTCTTCTGGCCATGTCGT+AGG - chr8_3:60050804-60050823 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 60050633 60051516 60050633 ID=Msa1299060;Name=Msa1299060
chr8_3 mRNA 60050633 60051516 60050633 ID=Msa1299060-mRNA-1;Parent=Msa1299060;Name=Msa1299060-mRNA-1;_AED=0.27;_eAED=0.30;_QI=0|0|0|1|1|1|2|0|264
chr8_3 exon 60050633 60051081 60050633 ID=Msa1299060-mRNA-1:exon:17453;Parent=Msa1299060-mRNA-1
chr8_3 exon 60051171 60051516 60051171 ID=Msa1299060-mRNA-1:exon:17454;Parent=Msa1299060-mRNA-1
chr8_3 CDS 60050633 60051081 60050633 ID=Msa1299060-mRNA-1:cds;Parent=Msa1299060-mRNA-1
chr8_3 CDS 60051171 60051516 60051171 ID=Msa1299060-mRNA-1:cds;Parent=Msa1299060-mRNA-1
Gene Sequence

>Msa1299060

ATGGGTCGCGCAAAAATACCTATGAAATACATCCAAAGTGCGAAATCTCGCAAAATAACTTTCAAACAAAGAAAGCATGGATTAGAAAAGAAAATTTCTGAATTTTGTAAGAAGTCTGGAGCAAAGGCTTGCTTTATTGTGTATGATGGTGATCATAATAATATTGAACCTACGACATGGCCAGAAGACCTTACAAGTGTAAACTCCTTGCTTCAAGAGTATCAGAATCAAAAGATTGAGAAAACTCCTGAGATATTTGATGTTAAGGACTACTTTGAAAATAAGATAGAAAAGGTTGAAACAAAGATTAACAAAGTGCGGAGAGATATCCTCACTAAGATCTACCCAACTTGGCATCCACATTTTAGTAACTTGGATGAGAATCAACTCAGAGACTTCATTGCTGCAATAAATGCTAAGATTCAAGCTTGCAATCACAAAATTAGCATTTCACCATCAAACCAACTTAGTGGGATTGTGGAGATGAATGACCAAGTAGTTAATCCTGATTATACAAGTGAGATGGTGGATTTCACAAGTCTTGGTGATTTGCCTCCTAGTTCATCATCAAACCAACTTAGTGGGCTTGTGGAGATGAGTGACCAAGTTGTTAATCCTGATTATAGGAGTGAGATTGTGGATTTCACTAGTCTTGGTGATTTGCCTCCAAGTTCATCAGCAAACCAACTTAGTCAACTTGTTGATCTTGATGACTGGATAAATCAACTAGATGATGGTGTTTTGTGCTGGGATTGTCAACCTGATGAGTTTTTTTGGAAAGATCTTTCTTTTTAA

Protein sequence

>Msa1299060

MGRAKIPMKYIQSAKSRKITFKQRKHGLEKKISEFCKKSGAKACFIVYDGDHNNIEPTTWPEDLTSVNSLLQEYQNQKIEKTPEIFDVKDYFENKIEKVETKINKVRRDILTKIYPTWHPHFSNLDENQLRDFIAAINAKIQACNHKISISPSNQLSGIVEMNDQVVNPDYTSEMVDFTSLGDLPPSSSSNQLSGLVEMSDQVVNPDYRSEIVDFTSLGDLPPSSSANQLSQLVDLDDWINQLDDGVLCWDCQPDEFFWKDLSF*