Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1302320 | AES91783.1 | 91.160 | 181 | 16 | 0 | 1 | 181 | 1 | 181 | 1.76e-112 | 328 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1302320 | G7JJ33 | 91.160 | 181 | 16 | 0 | 1 | 181 | 1 | 181 | 8.43e-113 | 328 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1302320 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1302320 | MtrunA17_Chr4g0066461 | 45.946 | 111 | 41 | 3 | 77 | 181 | 1 | 98 | 2.55e-21 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTCTCTGTTACGGTTAC+TGG | 0.183266 | 8_3:-64902149 | Msa1302320:CDS |
| AGTAACCGTAACAGAGAAAT+TGG | 0.195649 | 8_3:+64902151 | None:intergenic |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.266041 | 8_3:-64902125 | Msa1302320:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8_3:-64902178 | Msa1302320:CDS |
| GAGAGAGATTATGAGTTTGT+AGG | 0.295578 | 8_3:-64901940 | None:intergenic |
| ATCACTGCTTTGGTTCAAAA+TGG | 0.315294 | 8_3:-64901984 | Msa1302320:CDS |
| TAACAAATCAGTTTCTGAAA+TGG | 0.363545 | 8_3:-64902587 | Msa1302320:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8_3:-64901994 | Msa1302320:CDS |
| AACAAATCAGTTTCTGAAAT+GGG | 0.410347 | 8_3:-64902586 | Msa1302320:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8_3:+64902458 | None:intergenic |
| GAATCCCAATTTCTCTGTTA+CGG | 0.433893 | 8_3:-64902156 | Msa1302320:CDS |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8_3:-64902009 | Msa1302320:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8_3:-64902240 | Msa1302320:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8_3:+64902457 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8_3:-64902210 | Msa1302320:CDS |
| TGAAGAAAAGAAGAAAATGT+TGG | 0.489430 | 8_3:-64902252 | Msa1302320:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8_3:-64902023 | Msa1302320:CDS |
| TTTCTTTGGCATTGAATCTG+TGG | 0.500456 | 8_3:-64902111 | Msa1302320:CDS |
| TTCAACGTTTAGAACCTCTT+GGG | 0.500668 | 8_3:+64902534 | None:intergenic |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8_3:+64902473 | None:intergenic |
| GTTCAACGTTTAGAACCTCT+TGG | 0.509820 | 8_3:+64902533 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8_3:-64902101 | Msa1302320:CDS |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8_3:-64902212 | Msa1302320:CDS |
| AGAAAAGAAGAAAATGTTGG+AGG | 0.526690 | 8_3:-64902249 | Msa1302320:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8_3:-64902211 | Msa1302320:CDS |
| GTAACCGTAACAGAGAAATT+GGG | 0.551700 | 8_3:+64902152 | None:intergenic |
| TAAACGTTGAACGTGAAAGA+AGG | 0.570116 | 8_3:-64902522 | Msa1302320:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8_3:-64902209 | Msa1302320:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8_3:+64902083 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8_3:-64902135 | Msa1302320:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8_3:+64902465 | None:intergenic |
| TACACAGGAAGTGATTGTGA+AGG | 0.673700 | 8_3:-64902300 | Msa1302320:CDS |
| TTGGGATTCCTATTACGACA+CGG | 0.678110 | 8_3:+64902170 | None:intergenic |
| CACTACTACTAAAACCCAAG+AGG | 0.682406 | 8_3:-64902548 | Msa1302320:CDS |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8_3:-64902571 | Msa1302320:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | + | chr8_3:64902125-64902144 | None:intergenic | 15.0% |
| !!! | TTTTATTTTCATTCTTCATT+TGG | - | chr8_3:64902132-64902151 | Msa1302320:CDS | 15.0% |
| !!! | TTTATTTTCATTCTTCATTT+GGG | - | chr8_3:64902133-64902152 | Msa1302320:CDS | 15.0% |
| !! | ATAACAAATCAAACTATAAT+GGG | + | chr8_3:64902197-64902216 | None:intergenic | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | + | chr8_3:64902121-64902140 | None:intergenic | 20.0% |
| !! | TGCATTTAAACATGAAAAAT+TGG | + | chr8_3:64902162-64902181 | None:intergenic | 20.0% |
| !! | GATAACAAATCAAACTATAA+TGG | + | chr8_3:64902198-64902217 | None:intergenic | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | - | chr8_3:64902204-64902223 | Msa1302320:CDS | 20.0% |
| !! | TAACAAATCAGTTTCTGAAA+TGG | - | chr8_3:64901955-64901974 | Msa1302320:CDS | 25.0% |
| !!! | AACAAATCAGTTTCTGAAAT+GGG | - | chr8_3:64901956-64901975 | Msa1302320:CDS | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | - | chr8_3:64902203-64902222 | Msa1302320:CDS | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | - | chr8_3:64902218-64902237 | Msa1302320:CDS | 25.0% |
| ! | TGAAGAAAAGAAGAAAATGT+TGG | - | chr8_3:64902290-64902309 | Msa1302320:CDS | 25.0% |
| !! | TTTGATTACTGTGATTTTGA+AGG | - | chr8_3:64902464-64902483 | Msa1302320:CDS | 25.0% |
| ! | TAATGATGGTAATTTGATCT+TGG | - | chr8_3:64902533-64902552 | Msa1302320:CDS | 25.0% |
| GAATGATGTTAGTTACATTG+GGG | + | chr8_3:64902099-64902118 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | + | chr8_3:64902100-64902119 | None:intergenic | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | + | chr8_3:64902101-64902120 | None:intergenic | 30.0% | |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | - | chr8_3:64902227-64902246 | Msa1302320:CDS | 30.0% |
| AGAAAAGAAGAAAATGTTGG+AGG | - | chr8_3:64902293-64902312 | Msa1302320:CDS | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | - | chr8_3:64902330-64902349 | Msa1302320:intron | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | - | chr8_3:64902331-64902350 | Msa1302320:intron | 30.0% | |
| AAAATCACAGTAATCAAACC+AGG | + | chr8_3:64902462-64902481 | None:intergenic | 30.0% | |
| ! | GCTAATGTTTCTGTTAATGA+TGG | - | chr8_3:64902519-64902538 | Msa1302320:CDS | 30.0% |
| ! | TTCAACGTTTAGAACCTCTT+GGG | + | chr8_3:64902011-64902030 | None:intergenic | 35.0% |
| TAAACGTTGAACGTGAAAGA+AGG | - | chr8_3:64902020-64902039 | Msa1302320:CDS | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | - | chr8_3:64902332-64902351 | Msa1302320:intron | 35.0% | |
| AAATTAAAACCCCCTTCAAC+AGG | + | chr8_3:64902345-64902364 | None:intergenic | 35.0% | |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | - | chr8_3:64902347-64902366 | Msa1302320:intron | 35.0% |
| GAATCCCAATTTCTCTGTTA+CGG | - | chr8_3:64902386-64902405 | Msa1302320:intron | 35.0% | |
| GTAACCGTAACAGAGAAATT+GGG | + | chr8_3:64902393-64902412 | None:intergenic | 35.0% | |
| AGTAACCGTAACAGAGAAAT+TGG | + | chr8_3:64902394-64902413 | None:intergenic | 35.0% | |
| AATTTCTCTGTTACGGTTAC+TGG | - | chr8_3:64902393-64902412 | Msa1302320:intron | 35.0% | |
| ! | TTTCTTTGGCATTGAATCTG+TGG | - | chr8_3:64902431-64902450 | Msa1302320:intron | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | - | chr8_3:64902488-64902507 | Msa1302320:CDS | 35.0% |
| ! | ATCACTGCTTTGGTTCAAAA+TGG | - | chr8_3:64902558-64902577 | Msa1302320:CDS | 35.0% |
| GAAATGGGTAACAGAAAACG+TGG | - | chr8_3:64901971-64901990 | Msa1302320:CDS | 40.0% | |
| CACTACTACTAAAACCCAAG+AGG | - | chr8_3:64901994-64902013 | Msa1302320:CDS | 40.0% | |
| ! | GTTCAACGTTTAGAACCTCT+TGG | + | chr8_3:64902012-64902031 | None:intergenic | 40.0% |
| TTACATTGGGGTAGAGAGTT+GGG | + | chr8_3:64902087-64902106 | None:intergenic | 40.0% | |
| TACACAGGAAGTGATTGTGA+AGG | - | chr8_3:64902242-64902261 | Msa1302320:CDS | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | - | chr8_3:64902302-64902321 | Msa1302320:CDS | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | - | chr8_3:64902333-64902352 | Msa1302320:intron | 40.0% | |
| TTGGGATTCCTATTACGACA+CGG | + | chr8_3:64902375-64902394 | None:intergenic | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | - | chr8_3:64902417-64902436 | Msa1302320:intron | 40.0% | |
| ATTGAATCTGTGGCTAAACC+TGG | - | chr8_3:64902441-64902460 | Msa1302320:intron | 40.0% | |
| GTTACATTGGGGTAGAGAGT+TGG | + | chr8_3:64902088-64902107 | None:intergenic | 45.0% | |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | - | chr8_3:64902364-64902383 | Msa1302320:intron | 45.0% |
| !! | GGTTACTGGTTCAAGCAATG+TGG | - | chr8_3:64902407-64902426 | Msa1302320:intron | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | - | chr8_3:64902548-64902567 | Msa1302320:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | + | chr8_3:64902072-64902091 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | + | chr8_3:64902080-64902099 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 64901941 | 64902623 | 64901941 | ID=Msa1302320;Name=Msa1302320 |
| chr8_3 | mRNA | 64901941 | 64902623 | 64901941 | ID=Msa1302320-mRNA-1;Parent=Msa1302320;Name=Msa1302320-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|181 |
| chr8_3 | exon | 64902462 | 64902623 | 64902462 | ID=Msa1302320-mRNA-1:exon:19660;Parent=Msa1302320-mRNA-1 |
| chr8_3 | exon | 64901941 | 64902324 | 64901941 | ID=Msa1302320-mRNA-1:exon:19659;Parent=Msa1302320-mRNA-1 |
| chr8_3 | CDS | 64902462 | 64902623 | 64902462 | ID=Msa1302320-mRNA-1:cds;Parent=Msa1302320-mRNA-1 |
| chr8_3 | CDS | 64901941 | 64902324 | 64901941 | ID=Msa1302320-mRNA-1:cds;Parent=Msa1302320-mRNA-1 |
| Gene Sequence |
| Protein sequence |
