AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1302710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1302710 MtrunA17_Chr4g0074401 70.618 599 150 4 2 577 1 596 0.0 822
Msa1302710 MtrunA17_Chr4g0074411 70.051 591 158 4 1 572 1 591 0.0 802
Msa1302710 MtrunA17_Chr2g0304841 40.390 666 283 21 1 568 1 650 4.24e-123 378
Msa1302710 MtrunA17_Chr4g0074421 95.055 182 9 0 67 248 1 182 1.69e-113 336
Msa1302710 MtrunA17_Chr3g0137101 52.147 326 140 6 15 324 5 330 8.28e-93 298
Msa1302710 MtrunA17_Chr1g0149841 48.378 339 144 6 14 324 1 336 4.60e-92 291
Msa1302710 MtrunA17_Chr1g0161431 55.200 250 102 5 18 258 11 259 7.32e-84 274
Msa1302710 MtrunA17_Chr4g0067981 44.959 367 166 10 15 351 25 385 1.22e-81 269
Msa1302710 MtrunA17_Chr3g0123131 54.582 251 101 5 18 259 13 259 1.83e-80 263
Msa1302710 MtrunA17_Chr1g0149851 45.588 340 142 7 14 313 1 337 4.18e-78 256
Msa1302710 MtrunA17_Chr8g0376881 49.254 268 120 5 16 278 23 279 7.89e-78 255
Msa1302710 MtrunA17_Chr2g0294911 43.353 346 184 4 12 351 23 362 6.07e-77 255
Msa1302710 MtrunA17_Chr7g0275891 44.375 320 158 5 14 313 1 320 5.81e-75 242
Msa1302710 MtrunA17_Chr7g0275401 45.724 304 139 5 14 310 1 285 8.18e-75 240
Msa1302710 MtrunA17_Chr3g0134461 50.000 232 68 3 18 248 8 192 8.44e-58 192
Msa1302710 MtrunA17_Chr1g0149831 51.546 194 82 4 77 261 1 191 2.05e-52 179
Msa1302710 MtrunA17_Chr4g0009851 37.872 235 123 4 17 251 10 221 1.69e-49 174
Msa1302710 MtrunA17_Chr4g0009901 37.500 256 131 4 18 273 10 236 1.78e-47 167
Msa1302710 MtrunA17_Chr3g0134481 60.769 130 51 0 12 141 2 131 1.07e-46 162
Msa1302710 MtrunA17_Chr3g0134481 60.377 53 21 0 198 250 131 183 3.60e-15 74.3
Msa1302710 MtrunA17_Chr6g0471041 35.000 260 136 6 12 262 8 243 4.72e-42 156
Msa1302710 MtrunA17_Chr4g0009981 37.895 190 94 3 63 251 2 168 9.19e-36 134
Msa1302710 MtrunA17_Chr8g0391911 34.298 242 148 4 14 248 5 242 1.12e-30 120
Msa1302710 MtrunA17_Chr8g0350761 30.502 259 153 7 17 251 16 271 3.14e-28 118
Msa1302710 MtrunA17_Chr4g0074431 91.489 47 4 0 1 47 3 49 8.06e-24 94.7
Msa1302710 MtrunA17_Chr3g0127941 32.240 183 112 4 22 195 56 235 2.37e-23 104
Msa1302710 MtrunA17_Chr8g0383961 29.752 242 122 4 14 255 10 203 2.67e-23 99.8
Msa1302710 MtrunA17_Chr4g0011071 31.875 160 85 3 64 222 35 171 1.93e-22 94.7
Msa1302710 MtrunA17_Chr6g0473731 36.145 166 88 6 98 263 10 157 3.61e-22 99.8
Msa1302710 MtrunA17_Chr7g0276361 36.090 133 76 2 19 145 11 140 9.51e-22 99.4
Msa1302710 MtrunA17_Chr6g0456631 33.121 157 102 2 14 168 5 160 1.05e-21 92.4
Msa1302710 MtrunA17_Chr4g0009991 39.568 139 61 2 110 248 1 116 2.17e-21 90.1
Msa1302710 MtrunA17_Chr4g0030271 37.705 122 73 1 22 140 89 210 1.33e-19 93.2
Msa1302710 MtrunA17_Chr4g0031961 52.381 84 37 2 175 255 104 187 1.10e-18 87.4
Msa1302710 MtrunA17_Chr1g0181811 34.426 122 77 1 22 140 85 206 2.10e-18 89.4
Msa1302710 MtrunA17_Chr3g0091641 31.579 133 86 2 14 141 14 146 2.88e-18 88.6
Msa1302710 MtrunA17_Chr8g0345901 33.600 125 80 1 24 145 38 162 9.43e-18 87.0
Msa1302710 MtrunA17_Chr4g0061021 30.303 132 87 2 15 141 24 155 4.86e-17 84.7
Msa1302710 MtrunA17_Chr7g0255711 58.621 58 24 0 198 255 90 147 1.88e-16 80.5
Msa1302710 MtrunA17_Chr5g0400241 33.553 152 84 3 152 294 264 407 1.27e-15 80.1
Msa1302710 MtrunA17_Chr5g0400241 38.400 125 71 3 18 140 14 134 1.83e-15 79.3
Msa1302710 MtrunA17_Chr4g0054981 48.750 80 31 1 192 261 303 382 1.92e-15 79.3
Msa1302710 MtrunA17_Chr4g0054981 34.677 124 77 2 18 140 14 134 3.18e-14 75.5
Msa1302710 MtrunA17_Chr7g0223971 30.556 180 81 4 109 255 10 178 6.84e-15 77.4
Msa1302710 MtrunA17_Chr5g0423031 39.024 82 49 1 17 98 9 89 3.28e-14 71.2
Msa1302710 MtrunA17_Chr7g0274471 62.500 56 20 1 197 251 216 271 1.90e-13 72.8
Msa1302710 MtrunA17_Chr4g0040481 42.857 84 46 1 197 280 156 237 2.06e-13 72.4
Msa1302710 MtrunA17_Chr2g0324891 36.585 123 52 4 135 257 123 219 2.86e-13 72.0
Msa1302710 MtrunA17_Chr1g0190301 60.714 56 21 1 197 251 173 228 4.70e-13 71.2
Msa1302710 MtrunA17_Chr4g0010001 38.272 81 49 1 18 98 10 89 2.88e-12 62.8
Msa1302710 MtrunA17_Chr7g0261911 48.000 75 37 2 199 271 262 336 3.81e-12 68.9
Msa1302710 MtrunA17_Chr7g0256551 28.662 157 89 3 14 149 1 155 3.84e-12 66.6
Msa1302710 MtrunA17_Chr4g0042451 49.153 59 29 1 195 252 13 71 4.30e-12 67.8
Msa1302710 MtrunA17_Chr2g0291021 55.000 60 25 2 199 257 49 107 4.56e-12 67.4
Msa1302710 MtrunA17_Chr6g0461641 54.237 59 26 1 195 252 38 96 6.22e-12 67.8
Msa1302710 MtrunA17_Chr5g0417521 50.847 59 28 1 195 252 11 69 1.28e-11 66.2
Msa1302710 MtrunA17_Chr1g0198601 44.928 69 36 2 188 255 201 268 1.51e-11 66.2
Msa1302710 MtrunA17_Chr2g0321461 50.847 59 28 1 195 252 41 99 3.31e-11 65.5
Msa1302710 MtrunA17_Chr3g0095611 49.296 71 29 2 188 251 2 72 3.96e-11 62.8
Msa1302710 MtrunA17_Chr4g0062921 31.250 112 70 3 145 255 187 292 4.08e-11 65.5
Msa1302710 MtrunA17_Chr1g0162931 54.545 66 29 1 198 262 17 82 5.41e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1302710 AT2G25180.1 46.486 370 170 7 18 362 13 379 1.85e-89 288
Msa1302710 AT4G31920.1 50.000 318 122 7 18 311 13 317 8.63e-81 264
Msa1302710 AT1G67710.1 55.078 256 105 5 17 265 5 257 3.90e-80 261
Msa1302710 AT3G16857.1 44.311 334 157 4 9 314 24 356 7.37e-80 265
Msa1302710 AT5G58080.1 48.466 326 144 9 8 314 3 323 8.48e-80 264
Msa1302710 AT3G16857.2 44.311 334 157 4 9 314 24 356 1.28e-79 265
Msa1302710 AT2G01760.1 50.195 257 112 4 18 260 7 261 1.44e-69 229
Msa1302710 AT2G01760.2 45.263 285 112 5 18 260 7 289 1.79e-63 214
Msa1302710 AT3G16857.3 43.706 286 132 4 57 314 2 286 2.88e-63 219
Msa1302710 AT5G49240.1 41.633 245 131 5 12 248 32 272 8.63e-53 182
Msa1302710 AT1G67710.2 52.308 195 84 4 77 265 1 192 6.89e-52 184
Msa1302710 AT2G01760.3 48.990 198 85 4 77 260 1 196 2.21e-46 166
Msa1302710 AT3G62670.1 39.662 237 125 6 23 251 42 268 2.01e-39 149
Msa1302710 AT1G49190.1 30.667 300 168 8 18 291 30 315 3.72e-39 148
Msa1302710 AT1G67710.3 47.701 174 82 4 98 265 13 183 1.40e-37 145
Msa1302710 AT5G07210.2 29.965 287 155 5 22 267 16 297 7.15e-32 130
Msa1302710 AT5G07210.1 29.153 295 155 6 22 267 16 305 1.81e-29 123
Msa1302710 AT2G27070.1 29.965 287 158 8 22 267 16 300 1.74e-28 120
Msa1302710 AT1G49190.2 36.800 125 79 0 18 142 30 154 7.85e-22 100
Msa1302710 AT2G46790.3 38.211 123 71 2 22 140 37 158 1.15e-21 98.6
Msa1302710 AT2G46790.1 38.211 123 71 2 22 140 37 158 1.36e-21 98.6
Msa1302710 AT5G24470.1 36.000 125 75 2 21 141 49 172 1.43e-21 99.0
Msa1302710 AT5G60100.7 38.525 122 72 1 22 140 64 185 2.27e-19 91.3
Msa1302710 AT5G60100.6 38.525 122 72 1 22 140 64 185 2.27e-19 91.3
Msa1302710 AT5G60100.4 38.525 122 72 1 22 140 64 185 2.27e-19 91.3
Msa1302710 AT1G68210.1 24.444 270 170 4 22 258 13 281 2.49e-19 92.4
Msa1302710 AT5G60100.5 38.525 122 72 1 22 140 64 185 4.49e-19 90.9
Msa1302710 AT5G60100.3 38.525 122 72 1 22 140 64 185 4.49e-19 90.9
Msa1302710 AT5G60100.1 38.525 122 72 1 22 140 64 185 4.49e-19 90.9
Msa1302710 AT5G60100.2 38.525 122 72 1 22 140 64 185 7.21e-19 90.5
Msa1302710 AT5G05090.1 50.667 75 37 0 181 255 63 137 1.15e-17 83.6
Msa1302710 AT5G59570.2 39.623 106 62 1 152 257 96 199 1.45e-17 84.0
Msa1302710 AT5G59570.1 39.623 106 62 1 152 257 96 199 1.45e-17 84.0
Msa1302710 AT3G46640.2 58.333 60 25 0 198 257 143 202 2.28e-17 84.0
Msa1302710 AT3G46640.1 58.333 60 25 0 198 257 143 202 2.28e-17 84.0
Msa1302710 AT3G46640.3 60.345 58 23 0 198 255 143 200 3.47e-17 83.6
Msa1302710 AT5G02810.1 34.426 122 77 1 22 140 78 199 3.63e-17 85.9
Msa1302710 AT2G40970.1 58.621 58 24 0 198 255 104 161 5.02e-17 81.6
Msa1302710 AT3G10760.1 55.385 65 29 0 191 255 97 161 5.71e-17 82.8
Msa1302710 AT5G61380.1 31.818 132 85 2 15 141 10 141 8.04e-17 84.3
Msa1302710 AT5G44190.2 61.818 55 21 0 197 251 145 199 1.67e-15 78.6
Msa1302710 AT5G44190.1 61.818 55 21 0 197 251 145 199 1.80e-15 79.0
Msa1302710 AT4G18020.8 34.266 143 85 3 16 151 12 152 1.54e-13 73.6
Msa1302710 AT4G18020.11 34.266 143 85 3 16 151 12 152 1.54e-13 73.6
Msa1302710 AT4G18020.4 34.266 143 85 3 16 151 12 152 1.54e-13 73.6
Msa1302710 AT4G18020.5 34.266 143 85 3 16 151 12 152 1.54e-13 73.6
Msa1302710 AT4G18020.10 34.266 143 85 3 16 151 12 152 1.54e-13 73.6
Msa1302710 AT4G18020.7 34.266 143 85 3 16 151 12 152 1.54e-13 73.6
Msa1302710 AT4G18020.3 34.266 143 85 3 16 151 12 152 2.11e-13 73.6
Msa1302710 AT4G18020.2 34.266 143 85 3 16 151 12 152 2.11e-13 73.6
Msa1302710 AT4G18020.1 34.266 143 85 3 16 151 12 152 2.11e-13 73.6
Msa1302710 AT4G18020.6 34.266 143 85 3 16 151 12 152 2.11e-13 73.6
Msa1302710 AT4G18020.9 34.266 143 85 3 16 151 12 152 2.11e-13 73.6
Msa1302710 AT2G20570.1 54.098 61 28 0 197 257 151 211 7.89e-13 71.2
Msa1302710 AT5G45580.1 50.000 70 32 2 194 262 4 71 1.19e-12 68.9
Msa1302710 AT5G45580.2 50.000 70 32 2 194 262 20 87 1.23e-12 68.9
Msa1302710 AT2G20570.2 59.259 54 22 0 204 257 174 227 3.07e-12 69.3
Msa1302710 AT2G01060.1 55.000 60 25 2 199 257 15 73 5.11e-12 67.4
Msa1302710 AT3G04030.1 52.727 55 25 1 199 252 45 99 3.02e-11 65.9
Msa1302710 AT4G37180.1 51.667 60 28 1 197 255 208 267 3.19e-11 65.5
Msa1302710 AT3G04030.3 52.727 55 25 1 199 252 45 99 3.61e-11 65.9
Msa1302710 AT4G37180.2 51.667 60 28 1 197 255 215 274 3.67e-11 65.5
Msa1302710 AT5G06800.3 50.000 66 31 2 199 263 192 256 4.97e-11 64.3
Msa1302710 AT5G18240.2 54.545 55 24 1 199 252 45 99 5.34e-11 65.1
Msa1302710 AT5G18240.3 54.545 55 24 1 199 252 45 99 5.34e-11 65.1
Msa1302710 AT5G18240.5 54.545 55 24 1 199 252 45 99 5.99e-11 65.1
Msa1302710 AT5G18240.1 54.545 55 24 1 199 252 45 99 6.15e-11 65.1
Msa1302710 AT5G18240.4 54.545 55 24 1 199 252 45 99 6.15e-11 65.1

Find 112 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATTGGTGTCTCGAAAT+TGG 0.196977 8_3:-65394121 None:intergenic
GATTATCTTGCTTCTTCTAA+TGG 0.232806 8_3:+65394678 Msa1302710:CDS
CCACTTCTGACAGGGTTAAA+AGG 0.242635 8_3:-65394420 None:intergenic
GGACTTAAATTAATGTTATC+AGG 0.249835 8_3:-65394225 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.252390 8_3:+65393970 Msa1302710:CDS
AAAAGAAGCCTCGTTTAGTT+TGG 0.279239 8_3:+65393468 Msa1302710:CDS
CAATGTCAAGATGGCTTACT+TGG 0.323761 8_3:+65394588 Msa1302710:CDS
TGATTAATGAACTATTAGTT+TGG 0.331944 8_3:-65394731 None:intergenic
CCCGGCGGGTATGCGTGTTC+TGG 0.339385 8_3:+65391072 Msa1302710:CDS
CTCACTCAATGGAACAGAAT+TGG 0.343212 8_3:-65394460 None:intergenic
ATCATTAGTGATGGGGTATA+TGG 0.343848 8_3:+65393329 Msa1302710:CDS
AAAGAAGCCTCGTTTAGTTT+GGG 0.355798 8_3:+65393469 Msa1302710:CDS
CATTCCTTCCAAGTTCAAAC+TGG 0.363583 8_3:-65394055 None:intergenic
GATAAATATTCTGCTGAAAC+TGG 0.368030 8_3:-65394200 None:intergenic
CACACCATCCAGTTTGAACT+TGG 0.369083 8_3:+65394047 Msa1302710:CDS
TATCAAATGGTTATCTAAAC+AGG 0.370425 8_3:+65393912 Msa1302710:CDS
CTTCTCACATGCAACTATAA+TGG 0.375217 8_3:+65391139 Msa1302710:CDS
GTTTCTGCAGTTAATCTTGT+CGG 0.381222 8_3:+65393515 Msa1302710:CDS
AACATTGGTGTCTCGAAATT+GGG 0.390536 8_3:-65394120 None:intergenic
ATAAATCACTCTCCTGTTGA+AGG 0.396905 8_3:+65394378 Msa1302710:CDS
GAGCAATTGCTTAATGTAGA+TGG 0.399600 8_3:+65394825 Msa1302710:CDS
GATTAATGAACTATTAGTTT+GGG 0.399970 8_3:-65394730 None:intergenic
TCAGCAAGTCGGACTTGAAA+TGG 0.400458 8_3:+65393043 Msa1302710:CDS
TCCAGCACTTTGAGATAAGT+TGG 0.404451 8_3:-65391109 None:intergenic
AAATGTATCCAGCCATTTGC+AGG 0.408981 8_3:+65393712 Msa1302710:CDS
ACTGCATTTGAATCATCAAT+TGG 0.413674 8_3:-65394246 None:intergenic
TCAAGTTGAGAAGTTCTATT+CGG 0.435476 8_3:+65394764 Msa1302710:CDS
ATATAATTTGTACCTGCAAA+TGG 0.440246 8_3:-65393724 None:intergenic
TTCACAGCAGAACCTTCAAC+AGG 0.443727 8_3:-65394390 None:intergenic
TGAATTGTTGAGAGAAAAGA+AGG 0.450895 8_3:+65392959 Msa1302710:CDS
AGCTTGAAGCCATCCATGTC+TGG 0.455651 8_3:-65393017 None:intergenic
GAGAAGTTCTATTCGGATCA+AGG 0.456955 8_3:+65394771 Msa1302710:CDS
ATTCTAGTTCATGATGGTTG+TGG 0.457111 8_3:+65391009 None:intergenic
ACATGGCATTCAAACGTCAA+TGG 0.462461 8_3:+65394170 Msa1302710:CDS
AGAATTGGAAGCAAAGTTAG+AGG 0.465879 8_3:-65394445 None:intergenic
AGGGTTCAGTAGGTGGATAT+GGG 0.469935 8_3:+65393971 Msa1302710:CDS
TACTACAACTACGAAGTCAT+TGG 0.471545 8_3:+65392929 Msa1302710:CDS
TGACAGTGGCTCTGATGTCT+TGG 0.472341 8_3:-65394287 None:intergenic
CTTGACTTGATGAATGTTGA+AGG 0.474751 8_3:+65393677 Msa1302710:CDS
GATACAGAATTGATATCAAA+TGG 0.480940 8_3:+65393899 Msa1302710:CDS
TTAAGCTCTTCCATTCGAAT+CGG 0.481435 8_3:-65393248 None:intergenic
CAAGGAATCATGGAATCAAA+TGG 0.482889 8_3:+65394789 Msa1302710:CDS
TTCAGAATAGTCGTGTGCCT+TGG 0.483059 8_3:+65394853 Msa1302710:CDS
CATCGTTGCTGTTCCGTTGG+AGG 0.483957 8_3:+65394557 Msa1302710:CDS
CATTGCATCGTTTCCTCCAA+CGG 0.484353 8_3:-65394570 None:intergenic
TTCCTCCTTCAACAATACCT+TGG 0.487555 8_3:+65394656 Msa1302710:CDS
AATTTGTACCTGCAAATGGC+TGG 0.488638 8_3:-65393720 None:intergenic
TGGAAGAGCTTAAGAGCATA+TGG 0.489491 8_3:+65393258 Msa1302710:CDS
GTTGGATCCCTACCAGCAAA+AGG 0.495630 8_3:+65393951 Msa1302710:CDS
CAGCCGACAAAACATGTCAT+TGG 0.496819 8_3:-65394022 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8_3:+65393687 Msa1302710:CDS
TTGTTAAAATCCTCACTCAA+TGG 0.497939 8_3:-65394471 None:intergenic
TAGAAAGAAGATTGAAAGCA+AGG 0.500776 8_3:+65393292 Msa1302710:CDS
TTAGAGGAACCACTTCTGAC+AGG 0.501081 8_3:-65394429 None:intergenic
ATAATCCAAGGTATTGTTGA+AGG 0.508220 8_3:-65394661 None:intergenic
ATCAAGGTATCATTAGTGAT+GGG 0.510346 8_3:+65393321 Msa1302710:CDS
AATCAAGGTATCATTAGTGA+TGG 0.511362 8_3:+65393320 Msa1302710:CDS
TGTGTGATTTATAGTGTTGT+CGG 0.513795 8_3:+65393160 Msa1302710:intron
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8_3:+65393305 Msa1302710:CDS
CCATCCAGTTTGAACTTGGA+AGG 0.517311 8_3:+65394051 Msa1302710:CDS
AATGCAGTTACAGATTCCTC+TGG 0.518088 8_3:+65394261 Msa1302710:CDS
CTTTCACTTTCAGAAAACAT+TGG 0.525109 8_3:-65394135 None:intergenic
TCCAACTTATCTCAAAGTGC+TGG 0.528445 8_3:+65391108 Msa1302710:CDS
CAATTCTGTTCCATTGAGTG+AGG 0.541688 8_3:+65394461 Msa1302710:CDS
TGCTAACATCACTAATTACA+AGG 0.543736 8_3:-65392992 None:intergenic
TTCACTTATTGTGTTGTCCA+AGG 0.543954 8_3:-65394870 None:intergenic
CTATTCGGATCAAGGAATCA+TGG 0.544673 8_3:+65394779 Msa1302710:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.548262 8_3:+65393964 Msa1302710:CDS
TGGTTATCTAAACAGGATAG+AGG 0.553617 8_3:+65393919 Msa1302710:CDS
GCCAGAACACGCATACCCGC+CGG 0.553676 8_3:-65391073 None:intergenic
ATGGTTGTGGAGGATAGTTT+CGG 0.554649 8_3:+65391022 Msa1302710:CDS
CCTTCCAAGTTCAAACTGGA+TGG 0.554980 8_3:-65394051 None:intergenic
AGACATCGTTGCTGTTCCGT+TGG 0.560284 8_3:+65394554 Msa1302710:CDS
TTAGGAGAAGGTTTCCCGGC+GGG 0.561505 8_3:+65391058 Msa1302710:CDS
ATGTCATTGGATGCATATGC+TGG 0.565239 8_3:-65394009 None:intergenic
TAATGGAGACACTAGTTCCA+TGG 0.565315 8_3:+65394695 Msa1302710:CDS
TTTCAAGTCCGACTTGCTGA+AGG 0.572445 8_3:-65393040 None:intergenic
TTGGATCCCTACCAGCAAAA+GGG 0.576563 8_3:+65393952 Msa1302710:CDS
TTGACTTGATGAATGTTGAA+GGG 0.580486 8_3:+65393678 Msa1302710:CDS
GAAACGATGCAATGTCAAGA+TGG 0.582127 8_3:+65394579 Msa1302710:CDS
GGATAGAGGAGATGATGCGT+TGG 0.582696 8_3:+65393933 Msa1302710:CDS
TTGGGCTAAACTTTGTACCA+TGG 0.585108 8_3:-65394712 None:intergenic
AAGAAAGGTACAAAGTGAGG+AGG 0.585901 8_3:+65393406 Msa1302710:CDS
GAAAGTTCGAGTTCTTTACA+TGG 0.585974 8_3:+65394153 Msa1302710:CDS
TTCAAGCTCCTTCAGCAAGT+CGG 0.589889 8_3:+65393032 Msa1302710:CDS
AACATTTGCATTATTGACAG+TGG 0.591551 8_3:-65394301 None:intergenic
GGGTTCAGTAGGTGGATATG+GGG 0.592030 8_3:+65393972 Msa1302710:CDS
CTAGTTCATGATGGTTGTGG+AGG 0.595087 8_3:+65391012 None:intergenic
AGAAGAAGCAAGATAATCCA+AGG 0.597269 8_3:-65394673 None:intergenic
TGATGTTAGCATGCCAGACA+TGG 0.598210 8_3:+65393004 Msa1302710:CDS
TCTAGTGAAACCGATTCGAA+TGG 0.598674 8_3:+65393238 Msa1302710:CDS
ATGTTATGAGACTGAAGTGG+AGG 0.606395 8_3:-65394096 None:intergenic
GAATAGAACTTCTCAACTTG+AGG 0.607973 8_3:-65394762 None:intergenic
GCTAACAAGAATAAAACGCA+TGG 0.616521 8_3:+65393377 Msa1302710:CDS
GGTAAATGATGATATAGAGA+AGG 0.617327 8_3:+65393181 Msa1302710:CDS
AATGTCAAGATGGCTTACTT+GGG 0.618703 8_3:+65394589 Msa1302710:CDS
TACCAGCAAAAGGGTTCAGT+AGG 0.620378 8_3:+65393961 Msa1302710:CDS
TAGAGGAACCACTTCTGACA+GGG 0.620617 8_3:-65394428 None:intergenic
ATCCAAGGTATTGTTGAAGG+AGG 0.626426 8_3:-65394658 None:intergenic
GTCACATAAACTCACTGTCA+AGG 0.629956 8_3:-65393538 None:intergenic
TCAAGGTATCATTAGTGATG+GGG 0.639460 8_3:+65393322 Msa1302710:CDS
TGTGTGAGCTTACTGATGAA+AGG 0.640199 8_3:-65393071 None:intergenic
TCTGATGTCTTGGAAGCCAG+AGG 0.643398 8_3:-65394277 None:intergenic
CTTAAATTAATGTTATCAGG+CGG 0.647690 8_3:-65394222 None:intergenic
GTTAGCATGCCAGACATGGA+TGG 0.657571 8_3:+65393008 Msa1302710:CDS
TGAGCTTACTGATGAAAGGT+AGG 0.657693 8_3:-65393067 None:intergenic
GTGATGAAGAGTGTGATACA+TGG 0.658673 8_3:+65393203 Msa1302710:CDS
TGTTATGAGACTGAAGTGGA+GGG 0.664363 8_3:-65394095 None:intergenic
GGAATGTTATGAGACTGAAG+TGG 0.672395 8_3:-65394099 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8_3:+65393688 Msa1302710:CDS
TCATGATCCCAAACTAAACG+AGG 0.706682 8_3:-65393476 None:intergenic
TTTAGGAGAAGGTTTCCCGG+CGG 0.790759 8_3:+65391057 Msa1302710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATGATAGTAAATAAA+TGG - chr8_3:65392405-65392424 None:intergenic 10.0%
!!! TTAAAAAAATCTAAAACAAA+AGG + chr8_3:65392636-65392655 Msa1302710:intron 10.0%
!!! TAAAAAAATCTAAAACAAAA+GGG + chr8_3:65392637-65392656 Msa1302710:intron 10.0%
!! CAATTTACTAAAAAAAACTA+AGG - chr8_3:65391321-65391340 None:intergenic 15.0%
!!! CTTAGTTTTTTTTAGTAAAT+TGG + chr8_3:65391319-65391338 Msa1302710:intron 15.0%
!! AAATAAATGGAAATTATCTT+CGG - chr8_3:65392392-65392411 None:intergenic 15.0%
!!! AAACCTTTTTTTTTTCTTTG+AGG + chr8_3:65391280-65391299 Msa1302710:intron 20.0%
!!! TTTTTAGTAAATTGGTGATT+AGG + chr8_3:65391327-65391346 Msa1302710:intron 20.0%
!!! AAAAATTTGTTTGTTCAGTT+CGG + chr8_3:65391502-65391521 Msa1302710:intron 20.0%
!!! AGATGTGTTACAATTTTTTA+GGG - chr8_3:65391833-65391852 None:intergenic 20.0%
!! AATGATTATTACTGCTTTAT+TGG + chr8_3:65392703-65392722 Msa1302710:intron 20.0%
!!! ATGATTATTACTGCTTTATT+GGG + chr8_3:65392704-65392723 Msa1302710:intron 20.0%
!! GAAGAGACATTTAAATAAAA+AGG - chr8_3:65392869-65392888 None:intergenic 20.0%
!! ATAAGAAATGGATCTTAATT+AGG - chr8_3:65393124-65393143 None:intergenic 20.0%
!! TAAGATCCATTTCTTATATT+CGG + chr8_3:65393127-65393146 Msa1302710:intron 20.0%
!! AAATATCCGAATATAAGAAA+TGG - chr8_3:65393136-65393155 None:intergenic 20.0%
!!! ATTCTTGTTAGCAATATTTT+CGG - chr8_3:65393369-65393388 None:intergenic 20.0%
!! TGATGAATGATTCTTAAATA+TGG + chr8_3:65393590-65393609 Msa1302710:intron 20.0%
!!! TTTGAGATTTTTTGACAAAA+GGG - chr8_3:65393758-65393777 None:intergenic 20.0%
!!! GATTAATGAACTATTAGTTT+GGG - chr8_3:65394733-65394752 None:intergenic 20.0%
!! TGATTAATGAACTATTAGTT+TGG - chr8_3:65394734-65394753 None:intergenic 20.0%
! ATTGAATGAACGAAAAAGAA+AGG - chr8_3:65391172-65391191 None:intergenic 25.0%
! ACACCAATAAAACTTATTTG+TGG + chr8_3:65391393-65391412 Msa1302710:intron 25.0%
! AAGTTACTCAATAGTTATAG+TGG - chr8_3:65391570-65391589 None:intergenic 25.0%
!!! GAGATGTGTTACAATTTTTT+AGG - chr8_3:65391834-65391853 None:intergenic 25.0%
!! TTTTCTGCATCATTAAAAAC+CGG + chr8_3:65391876-65391895 Msa1302710:intron 25.0%
! GTTCTAGTATGATTTAAACA+TGG - chr8_3:65392020-65392039 None:intergenic 25.0%
! AACTACAACCTATTTATCAT+AGG + chr8_3:65392043-65392062 Msa1302710:intron 25.0%
! TTACATAGCCTATGATAAAT+AGG - chr8_3:65392054-65392073 None:intergenic 25.0%
! AGTGTATATTTATGCTTCAT+AGG + chr8_3:65392133-65392152 Msa1302710:intron 25.0%
!!! TTGAATAACTTTTCGATTTG+AGG + chr8_3:65392502-65392521 Msa1302710:intron 25.0%
! AAAAAAATCACTAACATGTC+AGG - chr8_3:65392730-65392749 None:intergenic 25.0%
!! TTTTTCCTTCTTTTTCTGAA+TGG + chr8_3:65392749-65392768 Msa1302710:intron 25.0%
!! AATTTTCATATGAGAATTGC+TGG + chr8_3:65392819-65392838 Msa1302710:intron 25.0%
!! GGTCTTTTATATAATAATGC+TGG + chr8_3:65392840-65392859 Msa1302710:intron 25.0%
!!! CAAAACAGATTTTAAGAGAA+AGG - chr8_3:65393097-65393116 None:intergenic 25.0%
! AGTTGAAGAAACTACATATT+AGG - chr8_3:65393627-65393646 None:intergenic 25.0%
!! ATCAAGTCAAGTATTTTCTT+AGG - chr8_3:65393668-65393687 None:intergenic 25.0%
!! ATATAATTTGTACCTGCAAA+TGG - chr8_3:65393727-65393746 None:intergenic 25.0%
!!! GTTTGAGATTTTTTGACAAA+AGG - chr8_3:65393759-65393778 None:intergenic 25.0%
! GATACAGAATTGATATCAAA+TGG + chr8_3:65393899-65393918 Msa1302710:CDS 25.0%
! TATCAAATGGTTATCTAAAC+AGG + chr8_3:65393912-65393931 Msa1302710:CDS 25.0%
! CTTAAATTAATGTTATCAGG+CGG - chr8_3:65394225-65394244 None:intergenic 25.0%
! GGACTTAAATTAATGTTATC+AGG - chr8_3:65394228-65394247 None:intergenic 25.0%
!! TTGGAAGTAGCTTTGATATA+AGG + chr8_3:65391257-65391276 Msa1302710:intron 30.0%
ACGCCTCAAAGAAAAAAAAA+AGG - chr8_3:65391286-65391305 None:intergenic 30.0%
!! GCTCCACAAATAAGTTTTAT+TGG - chr8_3:65391399-65391418 None:intergenic 30.0%
ACTCAATAGTTATAGTGGTA+CGG - chr8_3:65391565-65391584 None:intergenic 30.0%
CTTTGGCAAAAGAATGAATT+AGG - chr8_3:65391599-65391618 None:intergenic 30.0%
ATCCTAATAGTGTTTCTCTA+AGG + chr8_3:65391651-65391670 Msa1302710:intron 30.0%
TAGAAACCACAACAAAACTA+GGG - chr8_3:65391737-65391756 None:intergenic 30.0%
TTAGAAACCACAACAAAACT+AGG - chr8_3:65391738-65391757 None:intergenic 30.0%
TGTAGAGAGTAGAAAAACAA+GGG - chr8_3:65391762-65391781 None:intergenic 30.0%
ATGTAGAGAGTAGAAAAACA+AGG - chr8_3:65391763-65391782 None:intergenic 30.0%
TAAACCTTGTATCAGATGAA+AGG - chr8_3:65391792-65391811 None:intergenic 30.0%
ACTTCTGTCTCGTAAAAAAT+CGG - chr8_3:65391926-65391945 None:intergenic 30.0%
TCAAAACCACACCTAAAATT+GGG + chr8_3:65391947-65391966 Msa1302710:intron 30.0%
ATAGGCTATGTAAATGTATC+CGG + chr8_3:65392061-65392080 Msa1302710:intron 30.0%
! TTTTGCCATTCAGAAAAAGA+AGG - chr8_3:65392757-65392776 None:intergenic 30.0%
! TAAATCACTAGCTTGATTCT+TGG + chr8_3:65392891-65392910 Msa1302710:intron 30.0%
TGAATTGTTGAGAGAAAAGA+AGG + chr8_3:65392959-65392978 Msa1302710:CDS 30.0%
TGCTAACATCACTAATTACA+AGG - chr8_3:65392995-65393014 None:intergenic 30.0%
TGTGTGATTTATAGTGTTGT+CGG + chr8_3:65393160-65393179 Msa1302710:intron 30.0%
GGTAAATGATGATATAGAGA+AGG + chr8_3:65393181-65393200 Msa1302710:CDS 30.0%
TAGAAAGAAGATTGAAAGCA+AGG + chr8_3:65393292-65393311 Msa1302710:CDS 30.0%
! AATCAAGGTATCATTAGTGA+TGG + chr8_3:65393320-65393339 Msa1302710:CDS 30.0%
! ATCAAGGTATCATTAGTGAT+GGG + chr8_3:65393321-65393340 Msa1302710:CDS 30.0%
! AAAAAGAAAGGTACAAAGTG+AGG + chr8_3:65393403-65393422 Msa1302710:CDS 30.0%
TTCTTCAACTATTATTGCAG+AGG + chr8_3:65393637-65393656 Msa1302710:intron 30.0%
! TTGACTTGATGAATGTTGAA+GGG + chr8_3:65393678-65393697 Msa1302710:CDS 30.0%
TTTCAACCGTATTTGCAAAA+AGG - chr8_3:65393807-65393826 None:intergenic 30.0%
TGCAATCGAAAATGAAAATG+AGG - chr8_3:65393877-65393896 None:intergenic 30.0%
CTTTCACTTTCAGAAAACAT+TGG - chr8_3:65394138-65394157 None:intergenic 30.0%
GATAAATATTCTGCTGAAAC+TGG - chr8_3:65394203-65394222 None:intergenic 30.0%
ACTGCATTTGAATCATCAAT+TGG - chr8_3:65394249-65394268 None:intergenic 30.0%
AACATTTGCATTATTGACAG+TGG - chr8_3:65394304-65394323 None:intergenic 30.0%
TTGTTAAAATCCTCACTCAA+TGG - chr8_3:65394474-65394493 None:intergenic 30.0%
! TTTTGATGTTGTGAGTAACA+TGG - chr8_3:65394630-65394649 None:intergenic 30.0%
!! ATAATCCAAGGTATTGTTGA+AGG - chr8_3:65394664-65394683 None:intergenic 30.0%
GATTATCTTGCTTCTTCTAA+TGG + chr8_3:65394678-65394697 Msa1302710:CDS 30.0%
!! TCAAGTTGAGAAGTTCTATT+CGG + chr8_3:65394764-65394783 Msa1302710:CDS 30.0%
CTTCTCACATGCAACTATAA+TGG + chr8_3:65391139-65391158 Msa1302710:CDS 35.0%
GCAACGGTGTAAACATATAT+TGG + chr8_3:65391238-65391257 Msa1302710:intron 35.0%
!!! GGTGATTAGGTTAGTTTTCA+CGG + chr8_3:65391340-65391359 Msa1302710:intron 35.0%
!!! GTTAGTTTTCACGGTTTGAA+CGG + chr8_3:65391349-65391368 Msa1302710:intron 35.0%
ACTATTAGGATTGAGTCTCT+TGG - chr8_3:65391642-65391661 None:intergenic 35.0%
CACCTTAGAGAAACACTATT+AGG - chr8_3:65391656-65391675 None:intergenic 35.0%
TCTCTTTGCAACAAACACTA+GGG - chr8_3:65391702-65391721 None:intergenic 35.0%
TTCTCTTTGCAACAAACACT+AGG - chr8_3:65391703-65391722 None:intergenic 35.0%
!!! TACAAACCCTAGTTTTGTTG+TGG + chr8_3:65391728-65391747 Msa1302710:intron 35.0%
CTCAAAACCACACCTAAAAT+TGG + chr8_3:65391946-65391965 Msa1302710:intron 35.0%
CACTAATACATATCAGACTC+CGG - chr8_3:65392083-65392102 None:intergenic 35.0%
! TTTGAGGTAGCTTAGACATT+CGG - chr8_3:65392178-65392197 None:intergenic 35.0%
TACTACAACTACGAAGTCAT+TGG + chr8_3:65392929-65392948 Msa1302710:CDS 35.0%
TTAAGCTCTTCCATTCGAAT+CGG - chr8_3:65393251-65393270 None:intergenic 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8_3:65393305-65393324 Msa1302710:CDS 35.0%
! TCAAGGTATCATTAGTGATG+GGG + chr8_3:65393322-65393341 Msa1302710:CDS 35.0%
! ATCATTAGTGATGGGGTATA+TGG + chr8_3:65393329-65393348 Msa1302710:CDS 35.0%
! GTTAGCAATATTTTCGGATG+AGG - chr8_3:65393363-65393382 None:intergenic 35.0%
GCTAACAAGAATAAAACGCA+TGG + chr8_3:65393377-65393396 Msa1302710:CDS 35.0%
AACGCATGGACAAAAAAGAA+AGG + chr8_3:65393391-65393410 Msa1302710:CDS 35.0%
AAAAGAAGCCTCGTTTAGTT+TGG + chr8_3:65393468-65393487 Msa1302710:CDS 35.0%
! AAAGAAGCCTCGTTTAGTTT+GGG + chr8_3:65393469-65393488 Msa1302710:CDS 35.0%
GTTTCTGCAGTTAATCTTGT+CGG + chr8_3:65393515-65393534 Msa1302710:CDS 35.0%
! CTTGACTTGATGAATGTTGA+AGG + chr8_3:65393677-65393696 Msa1302710:CDS 35.0%
! TCACCTCCTTTTTGCAAATA+CGG + chr8_3:65393798-65393817 Msa1302710:intron 35.0%
TGGTTATCTAAACAGGATAG+AGG + chr8_3:65393919-65393938 Msa1302710:CDS 35.0%
! CATCCAATGACATGTTTTGT+CGG + chr8_3:65394019-65394038 Msa1302710:CDS 35.0%
! AACATTGGTGTCTCGAAATT+GGG - chr8_3:65394123-65394142 None:intergenic 35.0%
! AAACATTGGTGTCTCGAAAT+TGG - chr8_3:65394124-65394143 None:intergenic 35.0%
! GAAAGTTCGAGTTCTTTACA+TGG + chr8_3:65394153-65394172 Msa1302710:CDS 35.0%
ATAAATCACTCTCCTGTTGA+AGG + chr8_3:65394378-65394397 Msa1302710:CDS 35.0%
AGAATTGGAAGCAAAGTTAG+AGG - chr8_3:65394448-65394467 None:intergenic 35.0%
GTCGAAGAAAAATGAGAACT+GGG - chr8_3:65394534-65394553 None:intergenic 35.0%
TGTCGAAGAAAAATGAGAAC+TGG - chr8_3:65394535-65394554 None:intergenic 35.0%
AATGTCAAGATGGCTTACTT+GGG + chr8_3:65394589-65394608 Msa1302710:CDS 35.0%
AGAAGAAGCAAGATAATCCA+AGG - chr8_3:65394676-65394695 None:intergenic 35.0%
GAATAGAACTTCTCAACTTG+AGG - chr8_3:65394765-65394784 None:intergenic 35.0%
CAAGGAATCATGGAATCAAA+TGG + chr8_3:65394789-65394808 Msa1302710:CDS 35.0%
GAGCAATTGCTTAATGTAGA+TGG + chr8_3:65394825-65394844 Msa1302710:CDS 35.0%
ATGGTTGTGGAGGATAGTTT+CGG + chr8_3:65391022-65391041 Msa1302710:CDS 40.0%
!!! TTCGGTGTTGACGATGTTTT+AGG + chr8_3:65391040-65391059 Msa1302710:CDS 40.0%
!!! GTTGACGATGTTTTAGGAGA+AGG + chr8_3:65391046-65391065 Msa1302710:CDS 40.0%
!!! TGTTTTAGGAGAAGGTTTCC+CGG + chr8_3:65391054-65391073 Msa1302710:CDS 40.0%
TCCAACTTATCTCAAAGTGC+TGG + chr8_3:65391108-65391127 Msa1302710:CDS 40.0%
!! TCCAGCACTTTGAGATAAGT+TGG - chr8_3:65391112-65391131 None:intergenic 40.0%
ACTATTCTCCTCTCATGCAA+CGG + chr8_3:65391222-65391241 Msa1302710:intron 40.0%
ATCAAGCAACCAAAACAACG+TGG + chr8_3:65391425-65391444 Msa1302710:intron 40.0%
TCAAGCAACCAAAACAACGT+GGG + chr8_3:65391426-65391445 Msa1302710:intron 40.0%
TTGCAACAAACACTAGGGTT+GGG - chr8_3:65391697-65391716 None:intergenic 40.0%
TTTGCAACAAACACTAGGGT+TGG - chr8_3:65391698-65391717 None:intergenic 40.0%
CTCACCTTTCATCTGATACA+AGG + chr8_3:65391785-65391804 Msa1302710:intron 40.0%
! TCTGAAACTGCCAAAATCGA+CGG - chr8_3:65391993-65392012 None:intergenic 40.0%
TTGAGGTAGCTTAGACATTC+GGG - chr8_3:65392177-65392196 None:intergenic 40.0%
CAACTGATATGTGTGTGAGT+AGG - chr8_3:65392232-65392251 None:intergenic 40.0%
! TTCTTTTGCCAAGACTGTTG+TGG + chr8_3:65392284-65392303 Msa1302710:intron 40.0%
! TCTTTTGCCAAGACTGTTGT+GGG + chr8_3:65392285-65392304 Msa1302710:intron 40.0%
! TGTTGTGGGTGTGTTTGTTT+TGG + chr8_3:65392299-65392318 Msa1302710:intron 40.0%
TGAGCTTACTGATGAAAGGT+AGG - chr8_3:65393070-65393089 None:intergenic 40.0%
TGTGTGAGCTTACTGATGAA+AGG - chr8_3:65393074-65393093 None:intergenic 40.0%
! GTGATGAAGAGTGTGATACA+TGG + chr8_3:65393203-65393222 Msa1302710:CDS 40.0%
!! TCTAGTGAAACCGATTCGAA+TGG + chr8_3:65393238-65393257 Msa1302710:CDS 40.0%
! TGGAAGAGCTTAAGAGCATA+TGG + chr8_3:65393258-65393277 Msa1302710:CDS 40.0%
! AAGAAAGGTACAAAGTGAGG+AGG + chr8_3:65393406-65393425 Msa1302710:CDS 40.0%
! ACTAAACGAGGCTTCTTTTG+AGG - chr8_3:65393467-65393486 None:intergenic 40.0%
TCATGATCCCAAACTAAACG+AGG - chr8_3:65393479-65393498 None:intergenic 40.0%
GTCACATAAACTCACTGTCA+AGG - chr8_3:65393541-65393560 None:intergenic 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8_3:65393687-65393706 Msa1302710:CDS 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8_3:65393688-65393707 Msa1302710:CDS 40.0%
AAATGTATCCAGCCATTTGC+AGG + chr8_3:65393712-65393731 Msa1302710:CDS 40.0%
AATTTGTACCTGCAAATGGC+TGG - chr8_3:65393723-65393742 None:intergenic 40.0%
CAACCGTATTTGCAAAAAGG+AGG - chr8_3:65393804-65393823 None:intergenic 40.0%
ATGTCATTGGATGCATATGC+TGG - chr8_3:65394012-65394031 None:intergenic 40.0%
CATTCCTTCCAAGTTCAAAC+TGG - chr8_3:65394058-65394077 None:intergenic 40.0%
TGTTATGAGACTGAAGTGGA+GGG - chr8_3:65394098-65394117 None:intergenic 40.0%
ATGTTATGAGACTGAAGTGG+AGG - chr8_3:65394099-65394118 None:intergenic 40.0%
GGAATGTTATGAGACTGAAG+TGG - chr8_3:65394102-65394121 None:intergenic 40.0%
ACATGGCATTCAAACGTCAA+TGG + chr8_3:65394170-65394189 Msa1302710:CDS 40.0%
AATGCAGTTACAGATTCCTC+TGG + chr8_3:65394261-65394280 Msa1302710:CDS 40.0%
CTCACTCAATGGAACAGAAT+TGG - chr8_3:65394463-65394482 None:intergenic 40.0%
CAATTCTGTTCCATTGAGTG+AGG + chr8_3:65394461-65394480 Msa1302710:CDS 40.0%
GAAACGATGCAATGTCAAGA+TGG + chr8_3:65394579-65394598 Msa1302710:CDS 40.0%
CAATGTCAAGATGGCTTACT+TGG + chr8_3:65394588-65394607 Msa1302710:CDS 40.0%
TTCCTCCTTCAACAATACCT+TGG + chr8_3:65394656-65394675 Msa1302710:CDS 40.0%
!! ATCCAAGGTATTGTTGAAGG+AGG - chr8_3:65394661-65394680 None:intergenic 40.0%
TAATGGAGACACTAGTTCCA+TGG + chr8_3:65394695-65394714 Msa1302710:CDS 40.0%
TTGGGCTAAACTTTGTACCA+TGG - chr8_3:65394715-65394734 None:intergenic 40.0%
GAGAAGTTCTATTCGGATCA+AGG + chr8_3:65394771-65394790 Msa1302710:CDS 40.0%
CTATTCGGATCAAGGAATCA+TGG + chr8_3:65394779-65394798 Msa1302710:CDS 40.0%
TGTTTACACCGTTGCATGAG+AGG - chr8_3:65391233-65391252 None:intergenic 45.0%
CGGTTTGAACGGTTGTGAAT+CGG + chr8_3:65391360-65391379 Msa1302710:intron 45.0%
GGTCCAATACTGACCTACTT+TGG + chr8_3:65391523-65391542 Msa1302710:intron 45.0%
CCTCCAAAGTAGGTCAGTAT+TGG - chr8_3:65391529-65391548 None:intergenic 45.0%
CCAATACTGACCTACTTTGG+AGG + chr8_3:65391526-65391545 Msa1302710:intron 45.0%
TTGGTCACGGAAAGAGATTC+GGG - chr8_3:65391623-65391642 None:intergenic 45.0%
CTTGGTCACGGAAAGAGATT+CGG - chr8_3:65391624-65391643 None:intergenic 45.0%
AGGATTGAGTCTCTTGGTCA+CGG - chr8_3:65391636-65391655 None:intergenic 45.0%
GAAGTGAGAAAATCGGAGTC+CGG - chr8_3:65391898-65391917 None:intergenic 45.0%
! GGACTCCGATTTTCTCACTT+CGG + chr8_3:65391897-65391916 Msa1302710:intron 45.0%
! GACTCCGATTTTCTCACTTC+GGG + chr8_3:65391898-65391917 Msa1302710:intron 45.0%
! AGCAATCGAGGCCCAATTTT+AGG - chr8_3:65391961-65391980 None:intergenic 45.0%
! GTGTGAGTAGGTAGCTTTAC+TGG - chr8_3:65392220-65392239 None:intergenic 45.0%
AAACACACCCACAACAGTCT+TGG - chr8_3:65392295-65392314 None:intergenic 45.0%
TGATGTTAGCATGCCAGACA+TGG + chr8_3:65393004-65393023 Msa1302710:CDS 45.0%
TTCAAGCTCCTTCAGCAAGT+CGG + chr8_3:65393032-65393051 Msa1302710:CDS 45.0%
!! TTTCAAGTCCGACTTGCTGA+AGG - chr8_3:65393043-65393062 None:intergenic 45.0%
TCAGCAAGTCGGACTTGAAA+TGG + chr8_3:65393043-65393062 Msa1302710:CDS 45.0%
TTGGATCCCTACCAGCAAAA+GGG + chr8_3:65393952-65393971 Msa1302710:CDS 45.0%
! ACTGAACCCTTTTGCTGGTA+GGG - chr8_3:65393961-65393980 None:intergenic 45.0%
! TACTGAACCCTTTTGCTGGT+AGG - chr8_3:65393962-65393981 None:intergenic 45.0%
TACCAGCAAAAGGGTTCAGT+AGG + chr8_3:65393961-65393980 Msa1302710:CDS 45.0%
AAGGGTTCAGTAGGTGGATA+TGG + chr8_3:65393970-65393989 Msa1302710:CDS 45.0%
AGGGTTCAGTAGGTGGATAT+GGG + chr8_3:65393971-65393990 Msa1302710:CDS 45.0%
CAGCCGACAAAACATGTCAT+TGG - chr8_3:65394025-65394044 None:intergenic 45.0%
CACACCATCCAGTTTGAACT+TGG + chr8_3:65394047-65394066 Msa1302710:CDS 45.0%
CCTTCCAAGTTCAAACTGGA+TGG - chr8_3:65394054-65394073 None:intergenic 45.0%
CCATCCAGTTTGAACTTGGA+AGG + chr8_3:65394051-65394070 Msa1302710:CDS 45.0%
TTCACAGCAGAACCTTCAAC+AGG - chr8_3:65394393-65394412 None:intergenic 45.0%
CCACTTCTGACAGGGTTAAA+AGG - chr8_3:65394423-65394442 None:intergenic 45.0%
! CCTTTTAACCCTGTCAGAAG+TGG + chr8_3:65394420-65394439 Msa1302710:CDS 45.0%
TAGAGGAACCACTTCTGACA+GGG - chr8_3:65394431-65394450 None:intergenic 45.0%
TTAGAGGAACCACTTCTGAC+AGG - chr8_3:65394432-65394451 None:intergenic 45.0%
CATTGCATCGTTTCCTCCAA+CGG - chr8_3:65394573-65394592 None:intergenic 45.0%
TTCAGAATAGTCGTGTGCCT+TGG + chr8_3:65394853-65394872 Msa1302710:CDS 45.0%
!!! TTTTTTTTTTTTTTTTTTTG+AGG - chr8_3:65392194-65392213 None:intergenic 5.0%
!!! AAAATATTTTGTTAAAAAAA+TGG - chr8_3:65392540-65392559 None:intergenic 5.0%
!!! AAATTATATAAGTATTTTTT+TGG + chr8_3:65392587-65392606 Msa1302710:intron 5.0%
TTTAGGAGAAGGTTTCCCGG+CGG + chr8_3:65391057-65391076 Msa1302710:CDS 50.0%
AGCAACCAAAACAACGTGGG+TGG + chr8_3:65391429-65391448 Msa1302710:intron 50.0%
CGGAAAGAGATTCGGGACTT+TGG - chr8_3:65391616-65391635 None:intergenic 50.0%
!! TCGAGGCCCAATTTTAGGTG+TGG - chr8_3:65391956-65391975 None:intergenic 50.0%
!!! TCCAAATCGGCCGTCGATTT+TGG + chr8_3:65391980-65391999 Msa1302710:intron 50.0%
GTTAGCATGCCAGACATGGA+TGG + chr8_3:65393008-65393027 Msa1302710:CDS 50.0%
AGCTTGAAGCCATCCATGTC+TGG - chr8_3:65393020-65393039 None:intergenic 50.0%
GGATAGAGGAGATGATGCGT+TGG + chr8_3:65393933-65393952 Msa1302710:CDS 50.0%
GTTGGATCCCTACCAGCAAA+AGG + chr8_3:65393951-65393970 Msa1302710:CDS 50.0%
! CACCTACTGAACCCTTTTGC+TGG - chr8_3:65393966-65393985 None:intergenic 50.0%
CAGCAAAAGGGTTCAGTAGG+TGG + chr8_3:65393964-65393983 Msa1302710:CDS 50.0%
GGGTTCAGTAGGTGGATATG+GGG + chr8_3:65393972-65393991 Msa1302710:CDS 50.0%
TCTGATGTCTTGGAAGCCAG+AGG - chr8_3:65394280-65394299 None:intergenic 50.0%
!! TGACAGTGGCTCTGATGTCT+TGG - chr8_3:65394290-65394309 None:intergenic 50.0%
! AGACATCGTTGCTGTTCCGT+TGG + chr8_3:65394554-65394573 Msa1302710:CDS 50.0%
TTAGGAGAAGGTTTCCCGGC+GGG + chr8_3:65391058-65391077 Msa1302710:CDS 55.0%
! AGCTGCCACCCACGTTGTTT+TGG - chr8_3:65391437-65391456 None:intergenic 55.0%
GCTGCTCTCTCCTCCAAAGT+AGG - chr8_3:65391539-65391558 None:intergenic 55.0%
GGCGCCCGAAGTGAGAAAAT+CGG - chr8_3:65391905-65391924 None:intergenic 55.0%
GGGCCTCGATTGCTCCAAAT+CGG + chr8_3:65391967-65391986 Msa1302710:intron 55.0%
GCCAAAATCGACGGCCGATT+TGG - chr8_3:65391984-65392003 None:intergenic 55.0%
! CATCGTTGCTGTTCCGTTGG+AGG + chr8_3:65394557-65394576 Msa1302710:CDS 55.0%
!! CGGCCGATTTGGAGCAATCG+AGG - chr8_3:65391973-65391992 None:intergenic 60.0%
CCAGAACACGCATACCCGCC+GGG - chr8_3:65391075-65391094 None:intergenic 65.0%
GCCAGAACACGCATACCCGC+CGG - chr8_3:65391076-65391095 None:intergenic 65.0%
CCCGGCGGGTATGCGTGTTC+TGG + chr8_3:65391072-65391091 Msa1302710:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 65391019 65394887 65391019 ID=Msa1302710;Name=Msa1302710
chr8_3 mRNA 65391019 65394887 65391019 ID=Msa1302710-mRNA-1;Parent=Msa1302710;Name=Msa1302710-mRNA-1;_AED=0.07;_eAED=0.08;_QI=0|0|0|1|1|1|5|0|577
chr8_3 exon 65391019 65391160 65391019 ID=Msa1302710-mRNA-1:exon:19840;Parent=Msa1302710-mRNA-1
chr8_3 exon 65392928 65393080 65392928 ID=Msa1302710-mRNA-1:exon:19841;Parent=Msa1302710-mRNA-1
chr8_3 exon 65393174 65393545 65393174 ID=Msa1302710-mRNA-1:exon:19842;Parent=Msa1302710-mRNA-1
chr8_3 exon 65393657 65393733 65393657 ID=Msa1302710-mRNA-1:exon:19843;Parent=Msa1302710-mRNA-1
chr8_3 exon 65393898 65394887 65393898 ID=Msa1302710-mRNA-1:exon:19844;Parent=Msa1302710-mRNA-1
chr8_3 CDS 65391019 65391160 65391019 ID=Msa1302710-mRNA-1:cds;Parent=Msa1302710-mRNA-1
chr8_3 CDS 65392928 65393080 65392928 ID=Msa1302710-mRNA-1:cds;Parent=Msa1302710-mRNA-1
chr8_3 CDS 65393174 65393545 65393174 ID=Msa1302710-mRNA-1:cds;Parent=Msa1302710-mRNA-1
chr8_3 CDS 65393657 65393733 65393657 ID=Msa1302710-mRNA-1:cds;Parent=Msa1302710-mRNA-1
chr8_3 CDS 65393898 65394887 65393898 ID=Msa1302710-mRNA-1:cds;Parent=Msa1302710-mRNA-1
Gene Sequence

>Msa1302710

ATGATGGTTGTGGAGGATAGTTTCGGTGTTGACGATGTTTTAGGAGAAGGTTTCCCGGCGGGTATGCGTGTTCTGGCAGTTGATGACGATCCAACTTATCTCAAAGTGCTGGAGAAGCAACTTCTCACATGCAACTATAATGTTACTACAACTACGAAGTCATTGGAAGCGCTTGAATTGTTGAGAGAAAAGAAGGATATGTTTGACCTTGTAATTAGTGATGTTAGCATGCCAGACATGGATGGCTTCAAGCTCCTTCAGCAAGTCGGACTTGAAATGGACCTACCTTTCATCATGTTGTCGGTAAATGATGATATAGAGAAGGTGATGAAGAGTGTGATACATGGTGCTTGCAACTATCTAGTGAAACCGATTCGAATGGAAGAGCTTAAGAGCATATGGCAACATGTAGTTAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCATTAGTGATGGGGTATATGGTCAAGACACCTCATCCGAAAATATTGCTAACAAGAATAAAACGCATGGACAAAAAAGAAAGGTACAAAGTGAGGAGGAAGAAGCAGAACAAGATAATGATGAGAAACGTTCGCCTCAAAAGAAGCCTCGTTTAGTTTGGGATCATGAGTTGCATCGAAAATTTGTTTCTGCAGTTAATCTTGTCGGCCTTGACAAGGCTTCGCCTAAGAAAATACTTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTATCCAGCCATTTGCAGAGATACAGAATTGATATCAAATGGTTATCTAAACAGGATAGAGGAGATGATGCGTTGGATCCCTACCAGCAAAAGGGTTCAGTAGGTGGATATGGGGATTTTTGCACACTGCCAGCATATGCATCCAATGACATGTTTTGTCGGCTGAACACACCATCCAGTTTGAACTTGGAAGGAATGAGTTCTTCTGCACTAGTCCCTCCACTTCAGTCTCATAACATTCCCAATTTCGAGACACCAATGTTTTCTGAAAGTGAAAGTTCGAGTTCTTTACATGGCATTCAAACGTCAATGGAAATTAACCAGTTTCAGCAGAATATTTATCCGCCTGATAACATTAATTTAAGTCCAATTGATGATTCAAATGCAGTTACAGATTCCTCTGGCTTCCAAGACATCAGAGCCACTGTCAATAATGCAAATGTTTCTCTTTCTTGCATCTCAAGCAATCACATACAAACACATAAATCAGAAGCATTTATAAATCACTCTCCTGTTGAAGGTTCTGCTGTGAAAACACAACCTTTTAACCCTGTCAGAAGTGGTTCCTCTAACTTTGCTTCCAATTCTGTTCCATTGAGTGAGGATTTTAACAATGATCAAATGTTGCACAATAACTACAAGTTTACTTCTCCCAGTTCTCATTTTTCTTCGACAGACATCGTTGCTGTTCCGTTGGAGGAAACGATGCAATGTCAAGATGGCTTACTTGGGAATGTTATAAATGCTCCATGTTACTCACAACATCAAAATGCGAGTTCCTCCTTCAACAATACCTTGGATTATCTTGCTTCTTCTAATGGAGACACTAGTTCCATGGTACAAAGTTTAGCCCAAACTAATAGTTCATTAATCACTCAGATGCCTCAAGTTGAGAAGTTCTATTCGGATCAAGGAATCATGGAATCAAATGGTGATTGCTTTGTTGAGCAATTGCTTAATGTAGATGGATTCATTCAGAATAGTCGTGTGCCTTGGACAACACAATAA
Protein sequence

>Msa1302710

MMVVEDSFGVDDVLGEGFPAGMRVLAVDDDPTYLKVLEKQLLTCNYNVTTTTKSLEALELLREKKDMFDLVISDVSMPDMDGFKLLQQVGLEMDLPFIMLSVNDDIEKVMKSVIHGACNYLVKPIRMEELKSIWQHVVRKKIESKDQNQGIISDGVYGQDTSSENIANKNKTHGQKRKVQSEEEEAEQDNDEKRSPQKKPRLVWDHELHRKFVSAVNLVGLDKASPKKILDLMNVEGLTRENVSSHLQRYRIDIKWLSKQDRGDDALDPYQQKGSVGGYGDFCTLPAYASNDMFCRLNTPSSLNLEGMSSSALVPPLQSHNIPNFETPMFSESESSSSLHGIQTSMEINQFQQNIYPPDNINLSPIDDSNAVTDSSGFQDIRATVNNANVSLSCISSNHIQTHKSEAFINHSPVEGSAVKTQPFNPVRSGSSNFASNSVPLSEDFNNDQMLHNNYKFTSPSSHFSSTDIVAVPLEETMQCQDGLLGNVINAPCYSQHQNASSSFNNTLDYLASSNGDTSSMVQSLAQTNSSLITQMPQVEKFYSDQGIMESNGDCFVEQLLNVDGFIQNSRVPWTTQ*