AlfalfaGEDB | Msa1303290

M. sativa cultivar zhongmu-4 / Msa1303290

KEGG: K09338
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303290	sp\|P46602\|HAT3_ARATH	58.779	131	50	3	78	207	147	274	1.31e-39	141

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303290	G7JT79	93.182	220	11	2	1	220	1	216	7.79e-137	392

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TFs/TRs:

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Gene ID	Type	Classification
Msa1303290	TF	HB-HD-ZIP

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303290	MtrunA17_Chr4g0071411	93.182	220	11	2	1	220	1	216	1.50e-140	392
Msa1303290	MtrunA17_Chr8g0385491	84.466	103	12	2	1	103	83	181	5.70e-46	150
Msa1303290	MtrunA17_Chr5g0399491	53.741	147	58	2	61	207	112	248	1.50e-41	142
Msa1303290	MtrunA17_Chr5g0400781	63.077	130	36	4	80	207	115	234	1.55e-40	139
Msa1303290	MtrunA17_Chr4g0055851	55.000	140	54	2	80	213	102	238	3.35e-40	139
Msa1303290	MtrunA17_Chr8g0335131	63.248	117	40	1	91	207	145	258	4.71e-39	136
Msa1303290	MtrunA17_Chr1g0175651	49.367	158	70	3	50	207	118	265	1.27e-37	132
Msa1303290	MtrunA17_Chr2g0299031	43.961	207	94	5	11	215	15	201	1.72e-37	130
Msa1303290	MtrunA17_Chr7g0258831	48.101	158	73	2	50	207	123	271	2.10e-37	132
Msa1303290	MtrunA17_Chr4g0054421	60.684	117	42	2	91	207	126	238	7.51e-37	130

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303290	AT3G60390.1	58.779	131	50	3	78	207	147	274	1.33e-40	141
Msa1303290	AT4G37790.1	40.323	248	96	8	1	207	1	237	3.09e-40	139
Msa1303290	AT4G16780.1	57.143	126	50	2	81	206	119	240	3.74e-38	134
Msa1303290	AT5G06710.1	52.593	135	50	2	78	207	176	301	1.21e-37	134
Msa1303290	AT5G47370.1	50.704	142	65	2	78	217	116	254	6.86e-36	128
Msa1303290	AT2G44910.1	58.120	117	46	1	91	207	162	275	1.36e-35	128
Msa1303290	AT2G22800.1	39.331	239	98	7	1	207	1	224	1.44e-35	127
Msa1303290	AT4G17460.1	43.275	171	78	3	47	217	106	257	3.53e-35	126
Msa1303290	AT1G70920.2	42.958	142	68	2	67	207	14	143	2.88e-28	105
Msa1303290	AT1G70920.1	42.958	142	68	2	67	207	44	173	3.49e-28	106

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Find 35 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAACTGAGGCTAACAA+AGG	0.656758	8_3:-66365479	Msa1303290:CDS
AAGAGTGTTATGGAGCTTGA+AGG	0.507942	8_3:+66365432	None:intergenic
AAGATTAAAGAAGGAATTGC+AGG	0.404223	8_3:-66364719	Msa1303290:CDS
ACAGGTTTATGTCTAGGTCT+TGG	0.420666	8_3:-66365727	Msa1303290:CDS
ACAGTACTATGAGTCACCAC+TGG	0.648715	8_3:+66364643	None:intergenic
ACGATCGTAGAAAGAAACTG+AGG	0.720750	8_3:-66365489	Msa1303290:CDS
ACGTTCATAGGTTCTCCTTT+TGG	0.352374	8_3:+66365619	None:intergenic
ACGTTTATCATCAAAACCAG+TGG	0.690620	8_3:-66364659	Msa1303290:CDS
AGACAAGACAAATTGAGGTT+TGG	0.409339	8_3:-66365093	Msa1303290:CDS
AGTGATGAAGAATGCAATAC+AGG	0.485322	8_3:-66365745	Msa1303290:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTAGTGAATAAACCTGTGA+TGG	-	chr8_3:66364659-66364678	Msa1303290:CDS	30.0%
CAAAGGTGAAAGATTCAATT+TGG	-	chr8_3:66364758-66364777	Msa1303290:CDS	30.0%
ATGAATGAATGAATGGATAC+CGG	+	chr8_3:66364930-66364949	None:intergenic	30.0%
GTTAAAGACAAGACAAATTG+AGG	-	chr8_3:66365235-66365254	Msa1303290:intron	30.0%
AAGATTAAAGAAGGAATTGC+AGG	-	chr8_3:66365614-66365633	Msa1303290:CDS	30.0%
TGATCTTCATTATGCTTCAA+CGG	+	chr8_3:66365738-66365757	None:intergenic	30.0%
ATTAGTGAATAAACCTGTGA+TGG	-	chr8_3:66364659-66364678	Msa1303290:CDS	30.0%
CAAAGGTGAAAGATTCAATT+TGG	-	chr8_3:66364758-66364777	Msa1303290:CDS	30.0%
ATGAATGAATGAATGGATAC+CGG	+	chr8_3:66364930-66364949	None:intergenic	30.0%
GTTAAAGACAAGACAAATTG+AGG	-	chr8_3:66365235-66365254	Msa1303290:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	66364582	66365773	66364582	ID=Msa1303290;Name=Msa1303290
chr8_3	mRNA	66364582	66365773	66364582	ID=Msa1303290-mRNA-1;Parent=Msa1303290;Name=Msa1303290-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|1\|0.33\|1\|1\|1\|3\|0\|220
chr8_3	exon	66365426	66365773	66365426	ID=Msa1303290-mRNA-1:exon:20157;Parent=Msa1303290-mRNA-1
chr8_3	exon	66365073	66365152	66365073	ID=Msa1303290-mRNA-1:exon:20156;Parent=Msa1303290-mRNA-1
chr8_3	exon	66364582	66364816	66364582	ID=Msa1303290-mRNA-1:exon:20155;Parent=Msa1303290-mRNA-1
chr8_3	CDS	66365426	66365773	66365426	ID=Msa1303290-mRNA-1:cds;Parent=Msa1303290-mRNA-1
chr8_3	CDS	66365073	66365152	66365073	ID=Msa1303290-mRNA-1:cds;Parent=Msa1303290-mRNA-1
chr8_3	CDS	66364582	66364816	66364582	ID=Msa1303290-mRNA-1:cds;Parent=Msa1303290-mRNA-1

Gene Sequence

>Msa1303290

ATGGAAAGTGATGAAGAATGCAATACAGGTTTATGTCTAGGTCTTGGAATAGGTGCAACAAAGGTGAAGAAGCAGAAATTAGTGAATAAACCTGTGATGGCACCTTGTTTTGACCTAGCTTTTGAGCTTTGTCCAAAAGGAGAACCTATGAACGTACACAACAACAACAACAACAGCAAAGGTGAAAGATTCAATTTGGAGAGACACCAATATTATCAAAATGTAACGTGTAGCACTGATTCTGATAACACCAACAACAACAACGATCGTAGAAAGAAACTGAGGCTAACAAAGGAGCAATCATCCATGCTTGAGAGCACCTTCAAGCTCCATAACACTCTTAATCCGGTTCAGAAAATAGCACTTGCTGACCAATTGAGGTTAAAGACAAGACAAATTGAGGTTTGGTTTCAGAACAGGAGAGCAAGGACTAAGCTGAAGCAGATAGAGGTGGATTATGAGTTGTTGAAAAAACATTGTCAAAATCTAAGTGATGAGAACAAAAGATTAAAGAAGGAATTGCAGGAACTCAAAGTTGTTGGACAATTTCCGTTATGCCCTCAACGTTTATCATCAAAACCAGTGGTGACTCATAGTACTGTGTGCTCTTCTTGTGAGCAGAAACCGTTGAAGCATAATGAAGATCACAATAATAATTTATAA

Protein sequence

>Msa1303290

MESDEECNTGLCLGLGIGATKVKKQKLVNKPVMAPCFDLAFELCPKGEPMNVHNNNNNSKGERFNLERHQYYQNVTCSTDSDNTNNNNDRRKKLRLTKEQSSMLESTFKLHNTLNPVQKIALADQLRLKTRQIEVWFQNRRARTKLKQIEVDYELLKKHCQNLSDENKRLKKELQELKVVGQFPLCPQRLSSKPVVTHSTVCSSCEQKPLKHNEDHNNNL*