AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1303860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1303860 MtrunA17_Chr4g0070271 70.000 130 38 1 1 129 67 196 1.37e-55 172
Msa1303860 MtrunA17_Chr4g0070241 92.188 64 5 0 1 64 1 64 4.70e-37 121
Msa1303860 MtrunA17_Chr2g0301131 38.122 181 53 6 1 129 72 245 3.39e-28 103
Msa1303860 MtrunA17_Chr5g0399851 36.434 129 58 3 25 129 11 139 9.42e-20 79.3
Msa1303860 MtrunA17_Chr5g0399881 38.562 153 64 6 7 129 5 157 8.00e-19 77.4
Msa1303860 MtrunA17_Chr5g0399861 35.526 152 69 4 7 129 5 156 1.05e-17 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1303860 AT1G19180.2 35.256 156 66 4 2 122 17 172 5.16e-17 73.6
Msa1303860 AT1G19180.1 35.256 156 66 4 2 122 83 238 8.89e-17 74.3
Msa1303860 AT1G19180.3 35.256 156 66 4 2 122 83 238 8.89e-17 74.3

Find 23 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTCACTTCTTCGATTCA+TGG 0.237608 8_3:-67168591 Msa1303860:CDS
AATGTCAACTGTGCAGTTTC+CGG 0.323565 8_3:+67168885 None:intergenic
TTTAGCACCTAAAACAAGTA+TGG 0.324800 8_3:+67167745 None:intergenic
TCGGAATCGCTTCTTTGATA+TGG 0.333476 8_3:+67167799 None:intergenic
CAACCACCTCCTACACTAAC+TGG 0.374106 8_3:-67168747 Msa1303860:intron
TAATGGAGAAGTTATTGTAT+TGG 0.400656 8_3:-67168856 Msa1303860:CDS
GCTTCTTTGATATGGTGATT+TGG 0.408539 8_3:+67167807 None:intergenic
TAACTCAATGGTTAACAAAC+CGG 0.425259 8_3:-67168904 Msa1303860:CDS
GGTGGTTGAAGACTCATATC+AGG 0.439597 8_3:+67168762 None:intergenic
AGCTCCTCAACTTTGTCAGC+AGG 0.455494 8_3:+67168825 None:intergenic
TTACAGAACTAATAACTCAA+TGG 0.476118 8_3:-67168916 Msa1303860:intron
TTCTTCGATTCATGGAGAAA+AGG 0.478429 8_3:-67168583 Msa1303860:CDS
CAATTACCAGTTAGTGTAGG+AGG 0.522487 8_3:+67168741 None:intergenic
CTGTGGATTCTTTGTCTCCA+AGG 0.531713 8_3:-67169060 Msa1303860:intron
TCATGGAGAAAAGGAAAGCC+AGG 0.536399 8_3:-67168574 Msa1303860:intron
CATTTGCAAGCACTCAAACT+CGG 0.560353 8_3:-67168797 Msa1303860:CDS
AAGTATGGAGTGGATTCAGT+AGG 0.584677 8_3:+67167760 None:intergenic
CACCTAAAACAAGTATGGAG+TGG 0.594681 8_3:+67167750 None:intergenic
CTCATATCAGGAATAACTGA+AGG 0.598557 8_3:+67168774 None:intergenic
GTTTGTGGGAAATAAGTCCA+TGG 0.603000 8_3:+67169105 None:intergenic
TTACCAGTTAGTGTAGGAGG+TGG 0.608915 8_3:+67168744 None:intergenic
GAGCAATTACCAGTTAGTGT+AGG 0.610011 8_3:+67168738 None:intergenic
CTTGGAGACAAAGAATCCAC+AGG 0.648213 8_3:+67169061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTTTGAAACAAATAA+AGG - chr8_3:67168153-67168172 Msa1303860:intron 10.0%
!!! TAATATTTTGAAACAAATAA+AGG - chr8_3:67168153-67168172 Msa1303860:intron 10.0%
!!! AAGGAAATATTTTTTGTATT+AGG - chr8_3:67168172-67168191 Msa1303860:intron 15.0%
!!! AGGAAATATTTTTTGTATTA+GGG - chr8_3:67168173-67168192 Msa1303860:intron 15.0%
!! AAGAAATAAAATTTATGATC+AGG + chr8_3:67168218-67168237 None:intergenic 15.0%
!!! TTATTTCTTTAAAGAAGATA+TGG - chr8_3:67168229-67168248 Msa1303860:intron 15.0%
!!! TAAATTCAAGTTTTTATTCA+AGG - chr8_3:67168323-67168342 Msa1303860:intron 15.0%
!!! ATTATGAATATTTTGATTAC+CGG + chr8_3:67168554-67168573 None:intergenic 15.0%
!!! AAGGAAATATTTTTTGTATT+AGG - chr8_3:67168172-67168191 Msa1303860:intron 15.0%
!!! AGGAAATATTTTTTGTATTA+GGG - chr8_3:67168173-67168192 Msa1303860:intron 15.0%
!! AAGAAATAAAATTTATGATC+AGG + chr8_3:67168218-67168237 None:intergenic 15.0%
!!! TTATTTCTTTAAAGAAGATA+TGG - chr8_3:67168229-67168248 Msa1303860:intron 15.0%
!!! TAAATTCAAGTTTTTATTCA+AGG - chr8_3:67168323-67168342 Msa1303860:intron 15.0%
!!! ATTATGAATATTTTGATTAC+CGG + chr8_3:67168554-67168573 None:intergenic 15.0%
!! TAACAGTAACACTATATATT+AGG + chr8_3:67168999-67169018 None:intergenic 20.0%
!! TAACAGTAACACTATATATT+AGG + chr8_3:67168999-67169018 None:intergenic 20.0%
! TTACAGAACTAATAACTCAA+TGG - chr8_3:67167940-67167959 Msa1303860:intron 25.0%
!!! CAGTTGACATTGTTTTATAA+TGG - chr8_3:67167983-67168002 Msa1303860:intron 25.0%
! TAATGGAGAAGTTATTGTAT+TGG - chr8_3:67168000-67168019 Msa1303860:intron 25.0%
!!! TTGAATTTAATTTGTCTGAG+AGG + chr8_3:67168312-67168331 None:intergenic 25.0%
! TTGTTTCAACATTTCAATCA+AGG + chr8_3:67168438-67168457 None:intergenic 25.0%
!!! GTAAAATGTGATTTTTGAGA+TGG + chr8_3:67168787-67168806 None:intergenic 25.0%
! GTAACACTATATATTAGGTT+AGG + chr8_3:67168994-67169013 None:intergenic 25.0%
! TTACAGAACTAATAACTCAA+TGG - chr8_3:67167940-67167959 Msa1303860:intron 25.0%
!!! CAGTTGACATTGTTTTATAA+TGG - chr8_3:67167983-67168002 Msa1303860:intron 25.0%
! TAATGGAGAAGTTATTGTAT+TGG - chr8_3:67168000-67168019 Msa1303860:intron 25.0%
!!! TTGAATTTAATTTGTCTGAG+AGG + chr8_3:67168312-67168331 None:intergenic 25.0%
! TTGTTTCAACATTTCAATCA+AGG + chr8_3:67168438-67168457 None:intergenic 25.0%
!!! GTAAAATGTGATTTTTGAGA+TGG + chr8_3:67168787-67168806 None:intergenic 25.0%
! GTAACACTATATATTAGGTT+AGG + chr8_3:67168994-67169013 None:intergenic 25.0%
TAACTCAATGGTTAACAAAC+CGG - chr8_3:67167952-67167971 Msa1303860:intron 30.0%
GTTGTCGTAACTTTGTAATT+TGG - chr8_3:67168681-67168700 Msa1303860:intron 30.0%
!! AGTAGTTGATGATATGCATA+AGG + chr8_3:67168754-67168773 None:intergenic 30.0%
!! TCAATTTCTAGTAACACAAG+TGG + chr8_3:67168856-67168875 None:intergenic 30.0%
TAACTCAATGGTTAACAAAC+CGG - chr8_3:67167952-67167971 Msa1303860:intron 30.0%
GTTGTCGTAACTTTGTAATT+TGG - chr8_3:67168681-67168700 Msa1303860:intron 30.0%
!! AGTAGTTGATGATATGCATA+AGG + chr8_3:67168754-67168773 None:intergenic 30.0%
!! TCAATTTCTAGTAACACAAG+TGG + chr8_3:67168856-67168875 None:intergenic 30.0%
!!! TTTTTTGGTGTCACGTTTGT+GGG + chr8_3:67167768-67167787 None:intergenic 35.0%
! AGAAGCACAAAAACATACCT+TGG + chr8_3:67167816-67167835 None:intergenic 35.0%
!!! TTTTTTGCACACAAGCAATG+GGG + chr8_3:67167877-67167896 None:intergenic 35.0%
!!! CTTTTTTGCACACAAGCAAT+GGG + chr8_3:67167878-67167897 None:intergenic 35.0%
!!! ACTTTTTTGCACACAAGCAA+TGG + chr8_3:67167879-67167898 None:intergenic 35.0%
CTCATATCAGGAATAACTGA+AGG + chr8_3:67168085-67168104 None:intergenic 35.0%
TTCTTCGATTCATGGAGAAA+AGG - chr8_3:67168273-67168292 Msa1303860:intron 35.0%
!!! CAACTTTCGTGTTGTTTTCA+CGG + chr8_3:67168665-67168684 None:intergenic 35.0%
TAGAATTGGATGGAATCAGT+TGG + chr8_3:67168813-67168832 None:intergenic 35.0%
! AACTGATTCCATCCAATTCT+AGG - chr8_3:67168812-67168831 Msa1303860:CDS 35.0%
ACTGCAAAGTAAGTAACAAG+AGG + chr8_3:67168948-67168967 None:intergenic 35.0%
ATTTCCTGAGTGATGTTGTT+TGG - chr8_3:67168968-67168987 Msa1303860:intron 35.0%
GCTTCTTTGATATGGTGATT+TGG + chr8_3:67169052-67169071 None:intergenic 35.0%
!!! GTGGATTCAGTAGGTTTTTT+TGG + chr8_3:67169090-67169109 None:intergenic 35.0%
!!! TTTTTTGGTGTCACGTTTGT+GGG + chr8_3:67167768-67167787 None:intergenic 35.0%
! AGAAGCACAAAAACATACCT+TGG + chr8_3:67167816-67167835 None:intergenic 35.0%
!!! TTTTTTGCACACAAGCAATG+GGG + chr8_3:67167877-67167896 None:intergenic 35.0%
!!! CTTTTTTGCACACAAGCAAT+GGG + chr8_3:67167878-67167897 None:intergenic 35.0%
!!! ACTTTTTTGCACACAAGCAA+TGG + chr8_3:67167879-67167898 None:intergenic 35.0%
CTCATATCAGGAATAACTGA+AGG + chr8_3:67168085-67168104 None:intergenic 35.0%
TTCTTCGATTCATGGAGAAA+AGG - chr8_3:67168273-67168292 Msa1303860:intron 35.0%
!!! CAACTTTCGTGTTGTTTTCA+CGG + chr8_3:67168665-67168684 None:intergenic 35.0%
TAGAATTGGATGGAATCAGT+TGG + chr8_3:67168813-67168832 None:intergenic 35.0%
! AACTGATTCCATCCAATTCT+AGG - chr8_3:67168812-67168831 Msa1303860:CDS 35.0%
ACTGCAAAGTAAGTAACAAG+AGG + chr8_3:67168948-67168967 None:intergenic 35.0%
ATTTCCTGAGTGATGTTGTT+TGG - chr8_3:67168968-67168987 Msa1303860:intron 35.0%
GCTTCTTTGATATGGTGATT+TGG + chr8_3:67169052-67169071 None:intergenic 35.0%
!!! GTGGATTCAGTAGGTTTTTT+TGG + chr8_3:67169090-67169109 None:intergenic 35.0%
!!! GTTTTTTGGTGTCACGTTTG+TGG + chr8_3:67167769-67167788 None:intergenic 40.0%
ACCAAAAAACGTTACACCTG+TGG - chr8_3:67167779-67167798 Msa1303860:CDS 40.0%
!!! TCCACAGGTGTAACGTTTTT+TGG + chr8_3:67167783-67167802 None:intergenic 40.0%
GCAATGGGGCAAAACAAAAA+TGG + chr8_3:67167863-67167882 None:intergenic 40.0%
AATGTCAACTGTGCAGTTTC+CGG + chr8_3:67167974-67167993 None:intergenic 40.0%
! TTTTCCTGCTGACAAAGTTG+AGG - chr8_3:67168027-67168046 Msa1303860:intron 40.0%
CATTTGCAAGCACTCAAACT+CGG - chr8_3:67168059-67168078 Msa1303860:intron 40.0%
CAATTACCAGTTAGTGTAGG+AGG + chr8_3:67168118-67168137 None:intergenic 40.0%
GAGCAATTACCAGTTAGTGT+AGG + chr8_3:67168121-67168140 None:intergenic 40.0%
AGCTTCACTTCTTCGATTCA+TGG - chr8_3:67168265-67168284 Msa1303860:intron 40.0%
ATAGACAGAGACATGGAGAA+GGG + chr8_3:67168415-67168434 None:intergenic 40.0%
ATCAAGGATAGACAGAGACA+TGG + chr8_3:67168422-67168441 None:intergenic 40.0%
CAAGCAAAATCAAGGACTTC+CGG - chr8_3:67168532-67168551 Msa1303860:intron 40.0%
CACTATTTATGTTCCTCTCG+TGG + chr8_3:67168600-67168619 None:intergenic 40.0%
TTGTGCTCATGCTCTTACAA+GGG + chr8_3:67168626-67168645 None:intergenic 40.0%
!!! TTCGTGTTGTTTTCACGGTA+CGG + chr8_3:67168660-67168679 None:intergenic 40.0%
TCTAGTAACACAAGTGGCTT+TGG + chr8_3:67168850-67168869 None:intergenic 40.0%
CTCTAGTCCATTGTGCAAAT+TGG - chr8_3:67168918-67168937 Msa1303860:intron 40.0%
AGGCAAACCAATTTGCACAA+TGG + chr8_3:67168928-67168947 None:intergenic 40.0%
TCGGAATCGCTTCTTTGATA+TGG + chr8_3:67169060-67169079 None:intergenic 40.0%
!!! AGGTTTTTTTGGAGCAGAGT+CGG + chr8_3:67169079-67169098 None:intergenic 40.0%
! AAGTATGGAGTGGATTCAGT+AGG + chr8_3:67169099-67169118 None:intergenic 40.0%
!!! GTTTTTTGGTGTCACGTTTG+TGG + chr8_3:67167769-67167788 None:intergenic 40.0%
ACCAAAAAACGTTACACCTG+TGG - chr8_3:67167779-67167798 Msa1303860:CDS 40.0%
!!! TCCACAGGTGTAACGTTTTT+TGG + chr8_3:67167783-67167802 None:intergenic 40.0%
GCAATGGGGCAAAACAAAAA+TGG + chr8_3:67167863-67167882 None:intergenic 40.0%
AATGTCAACTGTGCAGTTTC+CGG + chr8_3:67167974-67167993 None:intergenic 40.0%
! TTTTCCTGCTGACAAAGTTG+AGG - chr8_3:67168027-67168046 Msa1303860:intron 40.0%
CATTTGCAAGCACTCAAACT+CGG - chr8_3:67168059-67168078 Msa1303860:intron 40.0%
CAATTACCAGTTAGTGTAGG+AGG + chr8_3:67168118-67168137 None:intergenic 40.0%
GAGCAATTACCAGTTAGTGT+AGG + chr8_3:67168121-67168140 None:intergenic 40.0%
AGCTTCACTTCTTCGATTCA+TGG - chr8_3:67168265-67168284 Msa1303860:intron 40.0%
ATAGACAGAGACATGGAGAA+GGG + chr8_3:67168415-67168434 None:intergenic 40.0%
ATCAAGGATAGACAGAGACA+TGG + chr8_3:67168422-67168441 None:intergenic 40.0%
CAAGCAAAATCAAGGACTTC+CGG - chr8_3:67168532-67168551 Msa1303860:intron 40.0%
CACTATTTATGTTCCTCTCG+TGG + chr8_3:67168600-67168619 None:intergenic 40.0%
TTGTGCTCATGCTCTTACAA+GGG + chr8_3:67168626-67168645 None:intergenic 40.0%
!!! TTCGTGTTGTTTTCACGGTA+CGG + chr8_3:67168660-67168679 None:intergenic 40.0%
TCTAGTAACACAAGTGGCTT+TGG + chr8_3:67168850-67168869 None:intergenic 40.0%
CTCTAGTCCATTGTGCAAAT+TGG - chr8_3:67168918-67168937 Msa1303860:intron 40.0%
AGGCAAACCAATTTGCACAA+TGG + chr8_3:67168928-67168947 None:intergenic 40.0%
TCGGAATCGCTTCTTTGATA+TGG + chr8_3:67169060-67169079 None:intergenic 40.0%
!!! AGGTTTTTTTGGAGCAGAGT+CGG + chr8_3:67169079-67169098 None:intergenic 40.0%
! AAGTATGGAGTGGATTCAGT+AGG + chr8_3:67169099-67169118 None:intergenic 40.0%
CTTGGAGACAAAGAATCCAC+AGG + chr8_3:67167798-67167817 None:intergenic 45.0%
CTGTGGATTCTTTGTCTCCA+AGG - chr8_3:67167796-67167815 Msa1303860:CDS 45.0%
GGTGGTTGAAGACTCATATC+AGG + chr8_3:67168097-67168116 None:intergenic 45.0%
TTACCAGTTAGTGTAGGAGG+TGG + chr8_3:67168115-67168134 None:intergenic 45.0%
TCATGGAGAAAAGGAAAGCC+AGG - chr8_3:67168282-67168301 Msa1303860:intron 45.0%
! ATTTGTCTGAGAGGCATACC+TGG + chr8_3:67168303-67168322 None:intergenic 45.0%
GATAGACAGAGACATGGAGA+AGG + chr8_3:67168416-67168435 None:intergenic 45.0%
! CTTGATTTTGCTTGCGTCGA+AGG + chr8_3:67168526-67168545 None:intergenic 45.0%
CTTCGACGCAAGCAAAATCA+AGG - chr8_3:67168524-67168543 Msa1303860:intron 45.0%
TCATAGTCAAGAACCACGAG+AGG - chr8_3:67168584-67168603 Msa1303860:CDS 45.0%
GTTGTGCTCATGCTCTTACA+AGG + chr8_3:67168627-67168646 None:intergenic 45.0%
TCAGCAAGCCTAGAATTGGA+TGG + chr8_3:67168823-67168842 None:intergenic 45.0%
CTCTTCAGCAAGCCTAGAAT+TGG + chr8_3:67168827-67168846 None:intergenic 45.0%
TAGGCCAAACAACATCACTC+AGG + chr8_3:67168975-67168994 None:intergenic 45.0%
CTTGGAGACAAAGAATCCAC+AGG + chr8_3:67167798-67167817 None:intergenic 45.0%
CTGTGGATTCTTTGTCTCCA+AGG - chr8_3:67167796-67167815 Msa1303860:CDS 45.0%
GGTGGTTGAAGACTCATATC+AGG + chr8_3:67168097-67168116 None:intergenic 45.0%
TTACCAGTTAGTGTAGGAGG+TGG + chr8_3:67168115-67168134 None:intergenic 45.0%
TCATGGAGAAAAGGAAAGCC+AGG - chr8_3:67168282-67168301 Msa1303860:intron 45.0%
! ATTTGTCTGAGAGGCATACC+TGG + chr8_3:67168303-67168322 None:intergenic 45.0%
GATAGACAGAGACATGGAGA+AGG + chr8_3:67168416-67168435 None:intergenic 45.0%
! CTTGATTTTGCTTGCGTCGA+AGG + chr8_3:67168526-67168545 None:intergenic 45.0%
CTTCGACGCAAGCAAAATCA+AGG - chr8_3:67168524-67168543 Msa1303860:intron 45.0%
TCATAGTCAAGAACCACGAG+AGG - chr8_3:67168584-67168603 Msa1303860:CDS 45.0%
GTTGTGCTCATGCTCTTACA+AGG + chr8_3:67168627-67168646 None:intergenic 45.0%
TCAGCAAGCCTAGAATTGGA+TGG + chr8_3:67168823-67168842 None:intergenic 45.0%
CTCTTCAGCAAGCCTAGAAT+TGG + chr8_3:67168827-67168846 None:intergenic 45.0%
TAGGCCAAACAACATCACTC+AGG + chr8_3:67168975-67168994 None:intergenic 45.0%
AGCTCCTCAACTTTGTCAGC+AGG + chr8_3:67168034-67168053 None:intergenic 50.0%
CAACCACCTCCTACACTAAC+TGG - chr8_3:67168109-67168128 Msa1303860:intron 50.0%
AGCTCCTCAACTTTGTCAGC+AGG + chr8_3:67168034-67168053 None:intergenic 50.0%
CAACCACCTCCTACACTAAC+TGG - chr8_3:67168109-67168128 Msa1303860:intron 50.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 67167753 67169125 67167753 ID=Msa1303860;Name=Msa1303860
chr8_3 mRNA 67167753 67169125 67167753 ID=Msa1303860-mRNA-1;Parent=Msa1303860;Name=Msa1303860-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0|0|0|0.75|1|1|4|0|130
chr8_3 exon 67169061 67169125 67169061 ID=Msa1303860-mRNA-1:exon:20536;Parent=Msa1303860-mRNA-1
chr8_3 exon 67168748 67168932 67168748 ID=Msa1303860-mRNA-1:exon:20535;Parent=Msa1303860-mRNA-1
chr8_3 exon 67168575 67168632 67168575 ID=Msa1303860-mRNA-1:exon:20534;Parent=Msa1303860-mRNA-1
chr8_3 exon 67167753 67167837 67167753 ID=Msa1303860-mRNA-1:exon:20533;Parent=Msa1303860-mRNA-1
chr8_3 CDS 67169061 67169125 67169061 ID=Msa1303860-mRNA-1:cds;Parent=Msa1303860-mRNA-1
chr8_3 CDS 67168748 67168932 67168748 ID=Msa1303860-mRNA-1:cds;Parent=Msa1303860-mRNA-1
chr8_3 CDS 67168575 67168632 67168575 ID=Msa1303860-mRNA-1:cds;Parent=Msa1303860-mRNA-1
chr8_3 CDS 67167753 67167837 67167753 ID=Msa1303860-mRNA-1:cds;Parent=Msa1303860-mRNA-1
Gene Sequence

>Msa1303860

ATGGACTTATTTCCCACAAACGTGACACCAAAAAACGTTACACCTGTGGATTCTTTGTCTCCAAGAACTAATAACTCAATGGTTAACAAACCGGAAACTGCACAGTTGACATTGTTTTATAATGGAGAAGTTATTGTATTGGATGATTTTCCTGCTGACAAAGTTGAGGAGCTTAAGTCATTTGCAAGCACTCAAACTCGGCCTTCAGTTATTCCTGATATGAGTCTTCAACCACCTCCTACACTAACTGATATGGTAATTGCTAGAAAAGCTTCACTTCTTCGATTCATGGAGAAAAGGAAAGCCAGAGTTTCTGCCAAATCACCATATCAAAGAAGCGATTCCGACTCTGCTCCAAAAAAACCTACTGAATCCACTCCATACTTGTTTTAG

Protein sequence

>Msa1303860

MDLFPTNVTPKNVTPVDSLSPRTNNSMVNKPETAQLTLFYNGEVIVLDDFPADKVEELKSFASTQTRPSVIPDMSLQPPPTLTDMVIARKASLLRFMEKRKARVSAKSPYQRSDSDSAPKKPTESTPYLF*