Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1306240 | A0A4P1QVC2 | 60.000 | 65 | 26 | 0 | 1 | 65 | 1 | 65 | 7.83e-16 | 76.6 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1306240 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1306240 | MtrunA17_Chr8g0351751 | 60.294 | 68 | 27 | 0 | 1 | 68 | 1 | 68 | 9.59e-18 | 73.9 |
| Msa1306240 | MtrunA17_Chr3g0107331 | 48.611 | 72 | 36 | 1 | 4 | 74 | 3 | 74 | 9.03e-17 | 69.7 |
| Msa1306240 | MtrunA17_Chr3g0107381 | 51.471 | 68 | 33 | 0 | 1 | 68 | 1 | 68 | 9.67e-17 | 70.9 |
| Msa1306240 | MtrunA17_Chr4g0013041 | 58.824 | 68 | 26 | 1 | 1 | 68 | 1 | 66 | 1.06e-16 | 70.5 |
| Msa1306240 | MtrunA17_Chr3g0107351 | 52.459 | 61 | 29 | 0 | 5 | 65 | 4 | 64 | 3.83e-15 | 65.5 |
| Msa1306240 | MtrunA17_Chr3g0107341 | 51.562 | 64 | 31 | 0 | 5 | 68 | 4 | 67 | 6.42e-15 | 65.1 |
| Msa1306240 | MtrunA17_Chr4g0014031 | 60.294 | 68 | 27 | 0 | 1 | 68 | 1 | 68 | 1.15e-14 | 65.5 |
| Msa1306240 | MtrunA17_Chr3g0107361 | 46.479 | 71 | 37 | 1 | 5 | 74 | 4 | 74 | 1.83e-14 | 63.9 |
| Msa1306240 | MtrunA17_Chr4g0014321 | 52.941 | 68 | 32 | 0 | 1 | 68 | 1 | 68 | 1.90e-13 | 62.4 |
| Msa1306240 | MtrunA17_Chr2g0283941 | 50.000 | 62 | 30 | 1 | 1 | 62 | 1 | 61 | 8.36e-13 | 60.8 |
| Msa1306240 | MtrunA17_Chr2g0296831 | 57.353 | 68 | 29 | 0 | 1 | 68 | 1 | 68 | 2.43e-12 | 57.4 |
| Msa1306240 | MtrunA17_Chr5g0432691 | 45.588 | 68 | 37 | 0 | 1 | 68 | 1 | 68 | 1.39e-11 | 56.2 |
| Msa1306240 | MtrunA17_Chr3g0110131 | 55.882 | 68 | 30 | 0 | 1 | 68 | 1 | 68 | 3.06e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1306240 | AT3G05860.2 | 39.706 | 68 | 41 | 0 | 1 | 68 | 1 | 68 | 3.99e-11 | 56.2 |
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAGCTTCATTTCTATAA+AGG | 0.239694 | 8_4:+1035457 | None:intergenic |
| CCAGAGTACTCTTAAAATTC+AGG | 0.294533 | 8_4:-1035376 | Msa1306240:CDS |
| ACAAGCTTCATTTCTATAAA+GGG | 0.333929 | 8_4:+1035458 | None:intergenic |
| GAAAAGCAACATACAAGAAA+AGG | 0.412608 | 8_4:-1035518 | Msa1306240:CDS |
| TCATTTCTATAAAGGGTGCT+TGG | 0.483092 | 8_4:+1035465 | None:intergenic |
| CCTGGTGATGGACAAATCTC+AGG | 0.489413 | 8_4:+1035405 | None:intergenic |
| AGAGTACTCTGGACTTCCCC+TGG | 0.519913 | 8_4:+1035387 | None:intergenic |
| TCTCAGGTTGTTGTTCATAA+CGG | 0.520016 | 8_4:+1035421 | None:intergenic |
| CTCTGGACTTCCCCTGGTGA+TGG | 0.527410 | 8_4:+1035393 | None:intergenic |
| CCTGAGATTTGTCCATCACC+AGG | 0.575929 | 8_4:-1035405 | Msa1306240:CDS |
| GAAGAATGATTTATTCGATG+AGG | 0.627361 | 8_4:-1035496 | Msa1306240:CDS |
| CTGAGATTTGTCCATCACCA+GGG | 0.642186 | 8_4:-1035404 | Msa1306240:CDS |
| TGAGATTTGTCCATCACCAG+GGG | 0.698659 | 8_4:-1035403 | Msa1306240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAGCTTCATTTCTATAAA+GGG | + | chr8_4:1035477-1035496 | None:intergenic | 25.0% |
| GAAAAGCAACATACAAGAAA+AGG | - | chr8_4:1035414-1035433 | Msa1306240:CDS | 30.0% | |
| GAAGAATGATTTATTCGATG+AGG | - | chr8_4:1035436-1035455 | Msa1306240:CDS | 30.0% | |
| CACAAGCTTCATTTCTATAA+AGG | + | chr8_4:1035478-1035497 | None:intergenic | 30.0% | |
| !! | TCATTTCTATAAAGGGTGCT+TGG | + | chr8_4:1035470-1035489 | None:intergenic | 35.0% |
| ! | TCTCAGGTTGTTGTTCATAA+CGG | + | chr8_4:1035514-1035533 | None:intergenic | 35.0% |
| !!! | CCTGAATTTTAAGAGTACTC+TGG | + | chr8_4:1035559-1035578 | None:intergenic | 35.0% |
| ! | CCAGAGTACTCTTAAAATTC+AGG | - | chr8_4:1035556-1035575 | Msa1306240:CDS | 35.0% |
| CTGAGATTTGTCCATCACCA+GGG | - | chr8_4:1035528-1035547 | Msa1306240:CDS | 45.0% | |
| TGAGATTTGTCCATCACCAG+GGG | - | chr8_4:1035529-1035548 | Msa1306240:CDS | 45.0% | |
| !! | CCTGGTGATGGACAAATCTC+AGG | + | chr8_4:1035530-1035549 | None:intergenic | 50.0% |
| CCTGAGATTTGTCCATCACC+AGG | - | chr8_4:1035527-1035546 | Msa1306240:CDS | 50.0% | |
| ! | AGAGTACTCTGGACTTCCCC+TGG | + | chr8_4:1035548-1035567 | None:intergenic | 55.0% |
| CTCTGGACTTCCCCTGGTGA+TGG | + | chr8_4:1035542-1035561 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 1035365 | 1035589 | 1035365 | ID=Msa1306240;Name=Msa1306240 |
| chr8_4 | mRNA | 1035365 | 1035589 | 1035365 | ID=Msa1306240-mRNA-1;Parent=Msa1306240;Name=Msa1306240-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|74 |
| chr8_4 | exon | 1035365 | 1035589 | 1035365 | ID=Msa1306240-mRNA-1:exon:196;Parent=Msa1306240-mRNA-1 |
| chr8_4 | CDS | 1035365 | 1035589 | 1035365 | ID=Msa1306240-mRNA-1:cds;Parent=Msa1306240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
