AlfalfaGEDB | Msa1306290

M. sativa cultivar zhongmu-4 / Msa1306290

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306290	QSD99710.1	95.556	180	7	1	1	180	1	179	1.13e-114	337

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306290	sp\|Q9S7G7\|MB3R1_ARATH	64.667	150	53	0	31	180	22	171	9.75e-67	220

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306290	A0A896WCC0	95.556	180	7	1	1	180	1	179	5.39e-115	337

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TFs/TRs:

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Gene ID	Type	Classification
Msa1306290	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306290	MtrunA17_Chr3g0139671	66.667	147	49	0	34	180	23	169	6.58e-67	219
Msa1306290	MtrunA17_Chr1g0157731	66.667	147	49	0	34	180	29	175	1.29e-66	219
Msa1306290	MtrunA17_Chr7g0238631	61.783	157	59	1	24	180	42	197	2.25e-64	207
Msa1306290	MtrunA17_Chr5g0397841	57.042	142	58	1	42	180	53	194	2.63e-51	171
Msa1306290	MtrunA17_Chr3g0117511	59.091	88	36	0	93	180	180	267	1.24e-29	112
Msa1306290	MtrunA17_Chr3g0117511	41.053	95	55	1	44	138	183	276	6.42e-16	74.7
Msa1306290	MtrunA17_Chr5g0429661	58.621	87	36	0	94	180	120	206	2.22e-29	110
Msa1306290	MtrunA17_Chr5g0429661	39.583	96	55	2	44	138	122	215	2.84e-15	72.4
Msa1306290	MtrunA17_Chr4g0058991	58.621	87	36	0	94	180	207	293	9.13e-29	110
Msa1306290	MtrunA17_Chr4g0058991	38.542	96	58	1	43	138	208	302	1.21e-14	70.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1306290	AT4G32730.1	64.667	150	53	0	31	180	22	171	9.91e-68	220
Msa1306290	AT4G32730.5	64.667	150	53	0	31	180	22	171	4.96e-67	221
Msa1306290	AT4G32730.4	64.667	150	53	0	31	180	22	171	4.96e-67	221
Msa1306290	AT4G32730.3	64.667	150	53	0	31	180	22	171	4.96e-67	221
Msa1306290	AT4G32730.2	64.667	150	53	0	31	180	22	171	4.96e-67	221
Msa1306290	AT5G02320.1	56.452	186	74	3	1	180	27	211	1.19e-66	213
Msa1306290	AT5G02320.2	56.452	186	74	3	1	180	27	211	1.19e-66	213
Msa1306290	AT5G02320.3	65.541	148	50	1	33	180	5	151	3.26e-66	210
Msa1306290	AT4G00540.2	54.737	190	76	3	1	180	1	190	2.96e-64	203
Msa1306290	AT4G00540.1	54.255	188	78	2	1	180	1	188	1.42e-63	202

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Find 41 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATGACAAAGATTTGAAT+CGG	0.447727	8_4:-1127692	Msa1306290:CDS
AACCGTTGTTGAATAGAGGC+AGG	0.565230	8_4:+1127618	None:intergenic
AACTCCATGGACCAAAGAAG+AGG	0.612896	8_4:-1125614	Msa1306290:CDS
AAGTCCTTACCAGGTCGCAT+AGG	0.617150	8_4:-1125799	Msa1306290:CDS
ACAAAATATATGGGAATAGT+TGG	0.337373	8_4:-1125570	Msa1306290:CDS
ACAAGAAGATCAACTAAAGG+AGG	0.608718	8_4:-1126739	Msa1306290:CDS
ACATGACAAAGATTTGAATC+GGG	0.560128	8_4:-1127691	Msa1306290:CDS
AGCGAGCTTGTTAAGATAAA+TGG	0.141546	8_4:-1125847	Msa1306290:CDS
AGGAGGCTGGACAGAGAAGG+AGG	0.589108	8_4:-1126722	Msa1306290:intron
AGGTCTTTGAGTTCCAAGAA+CGG	0.645576	8_4:+1127638	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATGACAAAGATTTGAATC+GGG	-	chr8_4:1125539-1125558	Msa1306290:CDS	30.0%
TGACTGAATAGAAATAGAAC+AGG	+	chr8_4:1125646-1125665	None:intergenic	30.0%
TCTTTATATTACAGCTGATG+CGG	-	chr8_4:1125791-1125810	Msa1306290:CDS	30.0%
TATTGGCAAGAAATTCTCTT+TGG	+	chr8_4:1126591-1126610	None:intergenic	30.0%
GGAAAAAGTTGGAAAAAGAT+AGG	-	chr8_4:1126703-1126722	Msa1306290:intron	30.0%
GCTATTGAAATAGTCAGATT+TGG	-	chr8_4:1126797-1126816	Msa1306290:intron	30.0%
TAGGTAAACAATGTAGAGAA+AGG	-	chr8_4:1127450-1127469	Msa1306290:intron	30.0%
CAGATATAAAGAAAACTCCA+TGG	-	chr8_4:1127603-1127622	Msa1306290:intron	30.0%
ACATGACAAAGATTTGAATC+GGG	-	chr8_4:1125539-1125558	Msa1306290:CDS	30.0%
TGACTGAATAGAAATAGAAC+AGG	+	chr8_4:1125646-1125665	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	1125534	1127718	1125534	ID=Msa1306290;Name=Msa1306290
chr8_4	mRNA	1125534	1127718	1125534	ID=Msa1306290-mRNA-1;Parent=Msa1306290;Name=Msa1306290-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0\|0\|0\|0.5\|1\|1\|6\|0\|181
chr8_4	exon	1127621	1127718	1127621	ID=Msa1306290-mRNA-1:exon:234;Parent=Msa1306290-mRNA-1
chr8_4	exon	1126723	1126777	1126723	ID=Msa1306290-mRNA-1:exon:233;Parent=Msa1306290-mRNA-1
chr8_4	exon	1126528	1126585	1126528	ID=Msa1306290-mRNA-1:exon:232;Parent=Msa1306290-mRNA-1
chr8_4	exon	1125995	1126089	1125995	ID=Msa1306290-mRNA-1:exon:231;Parent=Msa1306290-mRNA-1
chr8_4	exon	1125781	1125884	1125781	ID=Msa1306290-mRNA-1:exon:230;Parent=Msa1306290-mRNA-1
chr8_4	exon	1125534	1125669	1125534	ID=Msa1306290-mRNA-1:exon:229;Parent=Msa1306290-mRNA-1
chr8_4	CDS	1127621	1127718	1127621	ID=Msa1306290-mRNA-1:cds;Parent=Msa1306290-mRNA-1
chr8_4	CDS	1126723	1126777	1126723	ID=Msa1306290-mRNA-1:cds;Parent=Msa1306290-mRNA-1
chr8_4	CDS	1126528	1126585	1126528	ID=Msa1306290-mRNA-1:cds;Parent=Msa1306290-mRNA-1
chr8_4	CDS	1125995	1126089	1125995	ID=Msa1306290-mRNA-1:cds;Parent=Msa1306290-mRNA-1
chr8_4	CDS	1125781	1125884	1125781	ID=Msa1306290-mRNA-1:cds;Parent=Msa1306290-mRNA-1
chr8_4	CDS	1125534	1125669	1125534	ID=Msa1306290-mRNA-1:cds;Parent=Msa1306290-mRNA-1

Gene Sequence

>Msa1306290

ATGGAACATGACAAAGATTTGAATCGGGCATCAAGTTCTTCAGAACAAGAAAGCAATTCCGTTCTTGGAACTCAAAGACCTGCCTCTATTCAACAACGAAGGGTGACTGGTCCTACAAGAAGATCAACTAAAGGAGGCTGGACAGAGAAGGAGGATAAACTTTTGATTGCTGCTGTGAAAAAGTATAATGGAAAAAGTTGGAAAAAGATAGCTGCTTGTGTTACTGGCAGAACAGATGTTCAATGTCTGCATCGCTGGCAAAAGGTTCTGAATCCTGATCTTATCAAAGGACCTTGGTCAGAACAGGAAGATAATCAACTAAGCGAGCTTGTTAAGATAAATGGGAAAAAAAATTGGTCTCAGGTAGCAAAGTCCTTACCAGGTCGCATAGGTAAACAATGTAGAGAAAGGTGGTATAACCATTTAAATCCAGATATAAAGAAAACTCCATGGACCAAAGAAGAGGAGTCGACTCTAATCAAGACTCACAAAATATATGGGAATAGTTGGGCTGAAATAGCCAAATTTCTGCATGGAAGGTATTGA

Protein sequence

>Msa1306290

MEHDKDLNRASSSSEQESNSVLGTQRPASIQQRRVTGPTRRSTKGGWTEKEDKLLIAAVKKYNGKSWKKIAACVTGRTDVQCLHRWQKVLNPDLIKGPWSEQEDNQLSELVKINGKKNWSQVAKSLPGRIGKQCRERWYNHLNPDIKKTPWTKEEESTLIKTHKIYGNSWAEIAKFLHGRY*