AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1307050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307050 MtrunA17_Chr6g0452891 74.453 137 14 2 38 174 1 116 3.89e-64 196
Msa1307050 MtrunA17_Chr6g0452881 73.203 153 34 1 30 175 561 713 1.04e-63 209
Msa1307050 MtrunA17_Chr4g0037071 80.488 82 16 0 93 174 1 82 1.63e-43 144
Msa1307050 MtrunA17_Chr1g0179281 34.848 66 43 0 98 163 829 894 2.38e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307050 AT4G04885.1 63.000 100 37 0 76 175 591 690 6.54e-36 132
Msa1307050 AT1G66500.1 37.654 162 68 7 37 166 124 284 6.72e-23 94.4
Msa1307050 AT5G43620.1 37.342 158 68 7 37 166 123 277 4.18e-21 89.4

Find 75 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGAACCTTGAGAATATT+TGG 0.171658 8_4:+2234481 None:intergenic
GTGACTGCTGTGCTCATCTT+TGG 0.248435 8_4:+2234387 None:intergenic
GGTTGTTGGCTTGACGTATT+AGG 0.261888 8_4:+2234702 None:intergenic
AAGAGTTGTTTCCGATTCTT+TGG 0.310522 8_4:+2234790 None:intergenic
ATGCCTGTTGTTATTCCATT+AGG 0.318753 8_4:+2234073 None:intergenic
TCTTTGACTTCCTTCTTCAT+AGG 0.322887 8_4:+2233839 None:intergenic
ATGCATTTAGCATGAATAAT+GGG 0.333668 8_4:+2234031 None:intergenic
ATCGCATATATAGGTGTCTT+TGG 0.354576 8_4:-2235414 Msa1307050:CDS
TATGTAATTTGTGATTTATA+TGG 0.358933 8_4:-2234454 Msa1307050:CDS
CCGATTCTTTGGTATGGAAT+AGG 0.359303 8_4:+2234801 None:intergenic
TATGCATTTAGCATGAATAA+TGG 0.373205 8_4:+2234030 None:intergenic
TATATAGGTGTCTTTGGAAC+CGG 0.377723 8_4:-2235408 Msa1307050:CDS
GTCTTTGGAACCGGGCTATT+AGG 0.379584 8_4:-2235399 Msa1307050:CDS
GGAATAGGAAGTGGCATAAC+AGG 0.392358 8_4:+2234816 None:intergenic
GGCATAACAGGTCGTGCATT+TGG 0.398786 8_4:+2234828 None:intergenic
GTATATCTTAATGCGCCTAA+TGG 0.402277 8_4:-2234088 Msa1307050:three_prime_UTR
TAACAGGTCGTGCATTTGGC+AGG 0.413669 8_4:+2234832 None:intergenic
TGCCCAAGGTGTTATCTCAT+TGG 0.416501 8_4:-2234643 Msa1307050:CDS
AAATTAGAATATCCAACAAT+TGG 0.418071 8_4:+2234681 None:intergenic
AATATCCAACAATTGGTTGT+TGG 0.433148 8_4:+2234688 None:intergenic
TTTGCAGTAATCGCATATAT+AGG 0.434152 8_4:-2235423 None:intergenic
TTCCGACCTTTACCTCCAGC+TGG 0.442785 8_4:-2234768 Msa1307050:CDS
GATTCGGATGAGGTAGAATC+TGG 0.448129 8_4:+2234730 None:intergenic
AGTGAGACGATGTGGCTAAA+AGG 0.450066 8_4:-2234292 Msa1307050:three_prime_UTR
ATTCTACCTCATCCGAATCT+TGG 0.451748 8_4:-2234726 Msa1307050:CDS
TTGTTTCCGATTCTTTGGTA+TGG 0.469020 8_4:+2234795 None:intergenic
AGTAGAGCATGTGGGCCAGC+TGG 0.471650 8_4:+2234753 None:intergenic
TCAAGCCAACAACCAATTGT+TGG 0.475162 8_4:-2234693 Msa1307050:CDS
TCACTGTAAAACACGTCAAA+AGG 0.480580 8_4:+2234142 None:intergenic
CTTTGGTATGGAATAGGAAG+TGG 0.486169 8_4:+2234807 None:intergenic
GCTAAAAGGTGCAGAGACAT+TGG 0.487532 8_4:-2234278 Msa1307050:three_prime_UTR
CCTATTCCATACCAAAGAAT+CGG 0.490580 8_4:-2234801 Msa1307050:CDS
GACGTATTAGGACCAAGATT+CGG 0.494335 8_4:+2234714 None:intergenic
GCGCCTAATGGAATAACAAC+AGG 0.497466 8_4:-2234076 Msa1307050:three_prime_UTR
TGTGGGCCAGCTGGAGGTAA+AGG 0.498674 8_4:+2234762 None:intergenic
ATTGATTTAGTTACCTTCGC+AGG 0.505170 8_4:+2234609 None:intergenic
CATCTTTGGATTTGAATCGA+TGG 0.508546 8_4:+2234401 None:intergenic
TTGGGAGTTAAGTCAACTCC+AGG 0.511970 8_4:-2234259 Msa1307050:three_prime_UTR
ACAGTGATGAAACGGAGGAT+TGG 0.518817 8_4:-2234126 Msa1307050:three_prime_UTR
CCACAAGTTGTGCATTGTCT+AGG 0.520332 8_4:+2234424 None:intergenic
CTAAAAGGTGCAGAGACATT+GGG 0.523678 8_4:-2234277 Msa1307050:three_prime_UTR
AGAAAATAAAGATGAAGAGT+TGG 0.524053 8_4:-2234212 Msa1307050:three_prime_UTR
CCTAGACAATGCACAACTTG+TGG 0.528311 8_4:-2234424 Msa1307050:CDS
AGCACAGCAGTCACATGGAT+TGG 0.529243 8_4:-2234378 Msa1307050:CDS
AGCCAATGAGATAACACCTT+GGG 0.530219 8_4:+2234641 None:intergenic
TAAAATACATGTGCATTATG+TGG 0.538199 8_4:-2234169 Msa1307050:three_prime_UTR
AACAGGTCGTGCATTTGGCA+GGG 0.540179 8_4:+2234833 None:intergenic
AAAGAAAGCGCCTAATAGCC+CGG 0.541200 8_4:+2235389 None:intergenic
TTGATTTAGTTACCTTCGCA+GGG 0.565255 8_4:+2234610 None:intergenic
ATGCAGGGCACCTATGAAGA+AGG 0.565948 8_4:-2233849 Msa1307050:intron
ACAGGTCGTGCATTTGGCAG+GGG 0.566169 8_4:+2234834 None:intergenic
ATTAGTTCACTCATTGCCCA+AGG 0.573674 8_4:-2234657 Msa1307050:CDS
GAATATTTGGATCAAACTCA+AGG 0.580223 8_4:+2234494 None:intergenic
ATATAGGTGTCTTTGGAACC+GGG 0.582674 8_4:-2235407 Msa1307050:CDS
AGATGAGCACAGCAGTCACA+TGG 0.587427 8_4:-2234383 Msa1307050:CDS
GTTTGATCCAAATATTCTCA+AGG 0.588305 8_4:-2234488 Msa1307050:CDS
TAATGGAATAACAACAGGCA+TGG 0.604751 8_4:-2234071 Msa1307050:three_prime_UTR
AGAGCATGTGGGCCAGCTGG+AGG 0.611285 8_4:+2234756 None:intergenic
CAGGTCGTGCATTTGGCAGG+GGG 0.612021 8_4:+2234835 None:intergenic
GGCTAATCAAGCCCCTGCGA+AGG 0.612023 8_4:-2234622 Msa1307050:intron
GGCCAGCTGGAGGTAAAGGT+CGG 0.612329 8_4:+2234766 None:intergenic
AGAATCTGGAGTAGAGCATG+TGG 0.640782 8_4:+2234744 None:intergenic
TTAGGACCAAGATTCGGATG+AGG 0.644974 8_4:+2234720 None:intergenic
GTTGGCAGTTCCGTTCGATG+AGG 0.647423 8_4:-2234194 Msa1307050:three_prime_UTR
ACGGAGGATTGGATATGCAG+AGG 0.650743 8_4:-2234115 Msa1307050:three_prime_UTR
TAGCCAATGAGATAACACCT+TGG 0.659744 8_4:+2234640 None:intergenic
TGCATTTAGCATGAATAATG+GGG 0.666894 8_4:+2234032 None:intergenic
TATACCGCAAAATCTTCACA+CGG 0.678850 8_4:+2233995 None:intergenic
ATACCGCAAAATCTTCACAC+GGG 0.682049 8_4:+2233996 None:intergenic
TGATTTAGTTACCTTCGCAG+GGG 0.687811 8_4:+2234611 None:intergenic
GAATCTGGAGTAGAGCATGT+GGG 0.691664 8_4:+2234745 None:intergenic
ACTCACAAACTACTTGCGAG+AGG 0.695457 8_4:+2234312 None:intergenic
TACCGCAAAATCTTCACACG+GGG 0.732999 8_4:+2233997 None:intergenic
AGTTTGTGAGTGAGACGATG+TGG 0.738502 8_4:-2234300 Msa1307050:three_prime_UTR
GAAGTCAAAGAAAATGAACG+CGG 0.751197 8_4:-2233827 Msa1307050:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATCATATTAACAACT+TGG + chr8_4:2233942-2233961 None:intergenic 15.0%
!!! ATTAATTCATTTGCAATTTT+TGG - chr8_4:2234363-2234382 Msa1307050:CDS 15.0%
!!! TATGTAATTTGTGATTTATA+TGG - chr8_4:2234787-2234806 Msa1307050:CDS 15.0%
!! AACAAAAGAATAAATATTTC+CGG + chr8_4:2235361-2235380 None:intergenic 15.0%
!! TTAAAATCATATTAACAACT+TGG + chr8_4:2233942-2233961 None:intergenic 15.0%
!!! ATTAATTCATTTGCAATTTT+TGG - chr8_4:2234363-2234382 Msa1307050:CDS 15.0%
!!! TATGTAATTTGTGATTTATA+TGG - chr8_4:2234787-2234806 Msa1307050:CDS 15.0%
!! AACAAAAGAATAAATATTTC+CGG + chr8_4:2235361-2235380 None:intergenic 15.0%
!! ATGTTTCTTGAACAAAAAAT+TGG + chr8_4:2233879-2233898 None:intergenic 20.0%
!!! AATATGATTTTAACTATGAG+TGG - chr8_4:2233949-2233968 Msa1307050:intron 20.0%
!!! ATATGATTTTAACTATGAGT+GGG - chr8_4:2233950-2233969 Msa1307050:intron 20.0%
!!! TTTTTTGAAGTAATTTCATG+TGG + chr8_4:2234092-2234111 None:intergenic 20.0%
!! AAATTAGAATATCCAACAAT+TGG + chr8_4:2234563-2234582 None:intergenic 20.0%
!! ACAGAATACAATAACAAAAT+TGG + chr8_4:2234710-2234729 None:intergenic 20.0%
!! ATGTTTCTTGAACAAAAAAT+TGG + chr8_4:2233879-2233898 None:intergenic 20.0%
!!! AATATGATTTTAACTATGAG+TGG - chr8_4:2233949-2233968 Msa1307050:intron 20.0%
!!! ATATGATTTTAACTATGAGT+GGG - chr8_4:2233950-2233969 Msa1307050:intron 20.0%
!!! TTTTTTGAAGTAATTTCATG+TGG + chr8_4:2234092-2234111 None:intergenic 20.0%
!! AAATTAGAATATCCAACAAT+TGG + chr8_4:2234563-2234582 None:intergenic 20.0%
!! ACAGAATACAATAACAAAAT+TGG + chr8_4:2234710-2234729 None:intergenic 20.0%
! ATCATATTAACAACTTGGAA+AGG + chr8_4:2233937-2233956 None:intergenic 25.0%
! TCATTTCGCAAAACATTAAA+AGG - chr8_4:2234027-2234046 Msa1307050:three_prime_UTR 25.0%
! CAAAACATTAAAAGGAAAGA+AGG - chr8_4:2234035-2234054 Msa1307050:three_prime_UTR 25.0%
! ATAACTTATCCAATCATACT+CGG + chr8_4:2234313-2234332 None:intergenic 25.0%
! TGTTATTGTATTCTGTTTGT+AGG - chr8_4:2234714-2234733 Msa1307050:CDS 25.0%
!! TTCTGTTTGTAGGATTTTAT+TGG - chr8_4:2234724-2234743 Msa1307050:CDS 25.0%
!!! CTCTTCATCTTTATTTTCTT+CGG + chr8_4:2235028-2235047 None:intergenic 25.0%
! AGAAAATAAAGATGAAGAGT+TGG - chr8_4:2235029-2235048 Msa1307050:intron 25.0%
! TAAAATACATGTGCATTATG+TGG - chr8_4:2235072-2235091 Msa1307050:intron 25.0%
! ATGCATTTAGCATGAATAAT+GGG + chr8_4:2235213-2235232 None:intergenic 25.0%
! TATGCATTTAGCATGAATAA+TGG + chr8_4:2235214-2235233 None:intergenic 25.0%
!! TTGTTTTCATCATTATATGC+AGG - chr8_4:2235376-2235395 Msa1307050:CDS 25.0%
!! TGTTTTCATCATTATATGCA+GGG - chr8_4:2235377-2235396 Msa1307050:CDS 25.0%
! ATCATATTAACAACTTGGAA+AGG + chr8_4:2233937-2233956 None:intergenic 25.0%
! TCATTTCGCAAAACATTAAA+AGG - chr8_4:2234027-2234046 Msa1307050:three_prime_UTR 25.0%
! CAAAACATTAAAAGGAAAGA+AGG - chr8_4:2234035-2234054 Msa1307050:three_prime_UTR 25.0%
! ATAACTTATCCAATCATACT+CGG + chr8_4:2234313-2234332 None:intergenic 25.0%
! TGTTATTGTATTCTGTTTGT+AGG - chr8_4:2234714-2234733 Msa1307050:CDS 25.0%
!! TTCTGTTTGTAGGATTTTAT+TGG - chr8_4:2234724-2234743 Msa1307050:CDS 25.0%
!!! CTCTTCATCTTTATTTTCTT+CGG + chr8_4:2235028-2235047 None:intergenic 25.0%
! AGAAAATAAAGATGAAGAGT+TGG - chr8_4:2235029-2235048 Msa1307050:intron 25.0%
! TAAAATACATGTGCATTATG+TGG - chr8_4:2235072-2235091 Msa1307050:intron 25.0%
! ATGCATTTAGCATGAATAAT+GGG + chr8_4:2235213-2235232 None:intergenic 25.0%
! TATGCATTTAGCATGAATAA+TGG + chr8_4:2235214-2235233 None:intergenic 25.0%
!! TTGTTTTCATCATTATATGC+AGG - chr8_4:2235376-2235395 Msa1307050:CDS 25.0%
!! TGTTTTCATCATTATATGCA+GGG - chr8_4:2235377-2235396 Msa1307050:CDS 25.0%
!! TTGTTGATGATGCCAATTAA+TGG - chr8_4:2233978-2233997 Msa1307050:intron 30.0%
!! TGTTGATGATGCCAATTAAT+GGG - chr8_4:2233979-2233998 Msa1307050:intron 30.0%
AAATGAAGACTTAGGATACT+TGG + chr8_4:2234013-2234032 None:intergenic 30.0%
ATCTATTCTGATGTTTCATG+TGG + chr8_4:2234160-2234179 None:intergenic 30.0%
TATAATCCATGGGAAAATTC+AGG + chr8_4:2234207-2234226 None:intergenic 30.0%
! TGATTCGGATTATAATCCAT+GGG + chr8_4:2234217-2234236 None:intergenic 30.0%
AAGTGAAGATGAAACTGATT+CGG + chr8_4:2234232-2234251 None:intergenic 30.0%
AATATCCAACAATTGGTTGT+TGG + chr8_4:2234556-2234575 None:intergenic 30.0%
GAATATTTGGATCAAACTCA+AGG + chr8_4:2234750-2234769 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG - chr8_4:2234753-2234772 Msa1307050:CDS 30.0%
!! GCTTACAGTTTTTAGACATA+CGG + chr8_4:2234903-2234922 None:intergenic 30.0%
!!! TTCATCTTTATTTTCTTCGG+TGG + chr8_4:2235025-2235044 None:intergenic 30.0%
!!! GTATTTTAATCCTCATCGAA+CGG + chr8_4:2235060-2235079 None:intergenic 30.0%
TGCATTTAGCATGAATAATG+GGG + chr8_4:2235212-2235231 None:intergenic 30.0%
! AAGATTTTGCGGTATATGAA+GGG - chr8_4:2235253-2235272 Msa1307050:intron 30.0%
CATGATGTTATTATTGCAAG+TGG - chr8_4:2235309-2235328 Msa1307050:intron 30.0%
!! TTGTTGATGATGCCAATTAA+TGG - chr8_4:2233978-2233997 Msa1307050:intron 30.0%
!! TGTTGATGATGCCAATTAAT+GGG - chr8_4:2233979-2233998 Msa1307050:intron 30.0%
AAATGAAGACTTAGGATACT+TGG + chr8_4:2234013-2234032 None:intergenic 30.0%
ATCTATTCTGATGTTTCATG+TGG + chr8_4:2234160-2234179 None:intergenic 30.0%
TATAATCCATGGGAAAATTC+AGG + chr8_4:2234207-2234226 None:intergenic 30.0%
! TGATTCGGATTATAATCCAT+GGG + chr8_4:2234217-2234236 None:intergenic 30.0%
AAGTGAAGATGAAACTGATT+CGG + chr8_4:2234232-2234251 None:intergenic 30.0%
AATATCCAACAATTGGTTGT+TGG + chr8_4:2234556-2234575 None:intergenic 30.0%
GAATATTTGGATCAAACTCA+AGG + chr8_4:2234750-2234769 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG - chr8_4:2234753-2234772 Msa1307050:CDS 30.0%
!! GCTTACAGTTTTTAGACATA+CGG + chr8_4:2234903-2234922 None:intergenic 30.0%
!!! TTCATCTTTATTTTCTTCGG+TGG + chr8_4:2235025-2235044 None:intergenic 30.0%
!!! GTATTTTAATCCTCATCGAA+CGG + chr8_4:2235060-2235079 None:intergenic 30.0%
TGCATTTAGCATGAATAATG+GGG + chr8_4:2235212-2235231 None:intergenic 30.0%
! AAGATTTTGCGGTATATGAA+GGG - chr8_4:2235253-2235272 Msa1307050:intron 30.0%
CATGATGTTATTATTGCAAG+TGG - chr8_4:2235309-2235328 Msa1307050:intron 30.0%
ATCGCATATATAGGTGTCTT+TGG - chr8_4:2233827-2233846 Msa1307050:three_prime_UTR 35.0%
!! TATATAGGTGTCTTTGGAAC+CGG - chr8_4:2233833-2233852 Msa1307050:three_prime_UTR 35.0%
CTTCAGTCACATAACTTACT+TGG + chr8_4:2233907-2233926 None:intergenic 35.0%
!! GTTGATGATGCCAATTAATG+GGG - chr8_4:2233980-2233999 Msa1307050:intron 35.0%
TGGAAATGAACCCCATTAAT+TGG + chr8_4:2233993-2234012 None:intergenic 35.0%
! GTTTTGCGAAATGAAGACTT+AGG + chr8_4:2234021-2234040 None:intergenic 35.0%
AAGGAAAGAAGGAAATCTAG+AGG - chr8_4:2234046-2234065 Msa1307050:three_prime_UTR 35.0%
! TGATTACCTGAATTTTCCCA+TGG - chr8_4:2234198-2234217 Msa1307050:three_prime_UTR 35.0%
!! CTGATTCGGATTATAATCCA+TGG + chr8_4:2234218-2234237 None:intergenic 35.0%
TATTTGACAGTCGAGATTAG+AGG - chr8_4:2234282-2234301 Msa1307050:three_prime_UTR 35.0%
TGGTGATCTTTGCAATAGTT+TGG - chr8_4:2234383-2234402 Msa1307050:CDS 35.0%
CCTATTCCATACCAAAGAAT+CGG - chr8_4:2234440-2234459 Msa1307050:CDS 35.0%
TTGTTTCCGATTCTTTGGTA+TGG + chr8_4:2234449-2234468 None:intergenic 35.0%
! AAGAGTTGTTTCCGATTCTT+TGG + chr8_4:2234454-2234473 None:intergenic 35.0%
TTGATTTAGTTACCTTCGCA+GGG + chr8_4:2234634-2234653 None:intergenic 35.0%
! ATTGATTTAGTTACCTTCGC+AGG + chr8_4:2234635-2234654 None:intergenic 35.0%
ACGCAATCAAGTAAAATGGA+AGG + chr8_4:2234686-2234705 None:intergenic 35.0%
TGGTACGCAATCAAGTAAAA+TGG + chr8_4:2234690-2234709 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG + chr8_4:2234763-2234782 None:intergenic 35.0%
CATCTTTGGATTTGAATCGA+TGG + chr8_4:2234843-2234862 None:intergenic 35.0%
!! TTTATTTTCTTCGGTGGTCT+CGG + chr8_4:2235019-2235038 None:intergenic 35.0%
TCACTGTAAAACACGTCAAA+AGG + chr8_4:2235102-2235121 None:intergenic 35.0%
! CGTGTTTTACAGTGATGAAA+CGG - chr8_4:2235107-2235126 Msa1307050:intron 35.0%
GTATATCTTAATGCGCCTAA+TGG - chr8_4:2235153-2235172 Msa1307050:intron 35.0%
! ATGCCTGTTGTTATTCCATT+AGG + chr8_4:2235171-2235190 None:intergenic 35.0%
TAATGGAATAACAACAGGCA+TGG - chr8_4:2235170-2235189 Msa1307050:intron 35.0%
TATACCGCAAAATCTTCACA+CGG + chr8_4:2235249-2235268 None:intergenic 35.0%
! GAAGATTTTGCGGTATATGA+AGG - chr8_4:2235252-2235271 Msa1307050:intron 35.0%
! AGATTTTGCGGTATATGAAG+GGG - chr8_4:2235254-2235273 Msa1307050:intron 35.0%
! TCTTTGACTTCCTTCTTCAT+AGG + chr8_4:2235405-2235424 None:intergenic 35.0%
GAAGTCAAAGAAAATGAACG+CGG - chr8_4:2235414-2235433 Msa1307050:CDS 35.0%
ATCGCATATATAGGTGTCTT+TGG - chr8_4:2233827-2233846 Msa1307050:three_prime_UTR 35.0%
!! TATATAGGTGTCTTTGGAAC+CGG - chr8_4:2233833-2233852 Msa1307050:three_prime_UTR 35.0%
CTTCAGTCACATAACTTACT+TGG + chr8_4:2233907-2233926 None:intergenic 35.0%
!! GTTGATGATGCCAATTAATG+GGG - chr8_4:2233980-2233999 Msa1307050:intron 35.0%
TGGAAATGAACCCCATTAAT+TGG + chr8_4:2233993-2234012 None:intergenic 35.0%
! GTTTTGCGAAATGAAGACTT+AGG + chr8_4:2234021-2234040 None:intergenic 35.0%
AAGGAAAGAAGGAAATCTAG+AGG - chr8_4:2234046-2234065 Msa1307050:three_prime_UTR 35.0%
! TGATTACCTGAATTTTCCCA+TGG - chr8_4:2234198-2234217 Msa1307050:three_prime_UTR 35.0%
!! CTGATTCGGATTATAATCCA+TGG + chr8_4:2234218-2234237 None:intergenic 35.0%
TATTTGACAGTCGAGATTAG+AGG - chr8_4:2234282-2234301 Msa1307050:three_prime_UTR 35.0%
TGGTGATCTTTGCAATAGTT+TGG - chr8_4:2234383-2234402 Msa1307050:CDS 35.0%
CCTATTCCATACCAAAGAAT+CGG - chr8_4:2234440-2234459 Msa1307050:CDS 35.0%
TTGTTTCCGATTCTTTGGTA+TGG + chr8_4:2234449-2234468 None:intergenic 35.0%
! AAGAGTTGTTTCCGATTCTT+TGG + chr8_4:2234454-2234473 None:intergenic 35.0%
TTGATTTAGTTACCTTCGCA+GGG + chr8_4:2234634-2234653 None:intergenic 35.0%
! ATTGATTTAGTTACCTTCGC+AGG + chr8_4:2234635-2234654 None:intergenic 35.0%
ACGCAATCAAGTAAAATGGA+AGG + chr8_4:2234686-2234705 None:intergenic 35.0%
TGGTACGCAATCAAGTAAAA+TGG + chr8_4:2234690-2234709 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG + chr8_4:2234763-2234782 None:intergenic 35.0%
CATCTTTGGATTTGAATCGA+TGG + chr8_4:2234843-2234862 None:intergenic 35.0%
!! TTTATTTTCTTCGGTGGTCT+CGG + chr8_4:2235019-2235038 None:intergenic 35.0%
TCACTGTAAAACACGTCAAA+AGG + chr8_4:2235102-2235121 None:intergenic 35.0%
! CGTGTTTTACAGTGATGAAA+CGG - chr8_4:2235107-2235126 Msa1307050:intron 35.0%
GTATATCTTAATGCGCCTAA+TGG - chr8_4:2235153-2235172 Msa1307050:intron 35.0%
! ATGCCTGTTGTTATTCCATT+AGG + chr8_4:2235171-2235190 None:intergenic 35.0%
TAATGGAATAACAACAGGCA+TGG - chr8_4:2235170-2235189 Msa1307050:intron 35.0%
TATACCGCAAAATCTTCACA+CGG + chr8_4:2235249-2235268 None:intergenic 35.0%
! GAAGATTTTGCGGTATATGA+AGG - chr8_4:2235252-2235271 Msa1307050:intron 35.0%
! AGATTTTGCGGTATATGAAG+GGG - chr8_4:2235254-2235273 Msa1307050:intron 35.0%
! TCTTTGACTTCCTTCTTCAT+AGG + chr8_4:2235405-2235424 None:intergenic 35.0%
!! ATATAGGTGTCTTTGGAACC+GGG - chr8_4:2233834-2233853 Msa1307050:three_prime_UTR 40.0%
CTTTGGTATGGAATAGGAAG+TGG + chr8_4:2234437-2234456 None:intergenic 40.0%
CCGATTCTTTGGTATGGAAT+AGG + chr8_4:2234443-2234462 None:intergenic 40.0%
ATTCTACCTCATCCGAATCT+TGG - chr8_4:2234515-2234534 Msa1307050:intron 40.0%
GACGTATTAGGACCAAGATT+CGG + chr8_4:2234530-2234549 None:intergenic 40.0%
TCAAGCCAACAACCAATTGT+TGG - chr8_4:2234548-2234567 Msa1307050:intron 40.0%
ATTAGTTCACTCATTGCCCA+AGG - chr8_4:2234584-2234603 Msa1307050:intron 40.0%
AGCCAATGAGATAACACCTT+GGG + chr8_4:2234603-2234622 None:intergenic 40.0%
TAGCCAATGAGATAACACCT+TGG + chr8_4:2234604-2234623 None:intergenic 40.0%
TGATTTAGTTACCTTCGCAG+GGG + chr8_4:2234633-2234652 None:intergenic 40.0%
! CTAAAAGGTGCAGAGACATT+GGG - chr8_4:2234964-2234983 Msa1307050:intron 40.0%
! GTTTTACAGTGATGAAACGG+AGG - chr8_4:2235110-2235129 Msa1307050:intron 40.0%
ATACCGCAAAATCTTCACAC+GGG + chr8_4:2235248-2235267 None:intergenic 40.0%
!! AAGTGGACTTTTGCATATGC+TGG - chr8_4:2235326-2235345 Msa1307050:intron 40.0%
!! ATATAGGTGTCTTTGGAACC+GGG - chr8_4:2233834-2233853 Msa1307050:three_prime_UTR 40.0%
CTTTGGTATGGAATAGGAAG+TGG + chr8_4:2234437-2234456 None:intergenic 40.0%
CCGATTCTTTGGTATGGAAT+AGG + chr8_4:2234443-2234462 None:intergenic 40.0%
ATTCTACCTCATCCGAATCT+TGG - chr8_4:2234515-2234534 Msa1307050:intron 40.0%
GACGTATTAGGACCAAGATT+CGG + chr8_4:2234530-2234549 None:intergenic 40.0%
TCAAGCCAACAACCAATTGT+TGG - chr8_4:2234548-2234567 Msa1307050:intron 40.0%
ATTAGTTCACTCATTGCCCA+AGG - chr8_4:2234584-2234603 Msa1307050:intron 40.0%
AGCCAATGAGATAACACCTT+GGG + chr8_4:2234603-2234622 None:intergenic 40.0%
TAGCCAATGAGATAACACCT+TGG + chr8_4:2234604-2234623 None:intergenic 40.0%
TGATTTAGTTACCTTCGCAG+GGG + chr8_4:2234633-2234652 None:intergenic 40.0%
! CTAAAAGGTGCAGAGACATT+GGG - chr8_4:2234964-2234983 Msa1307050:intron 40.0%
! GTTTTACAGTGATGAAACGG+AGG - chr8_4:2235110-2235129 Msa1307050:intron 40.0%
ATACCGCAAAATCTTCACAC+GGG + chr8_4:2235248-2235267 None:intergenic 40.0%
!! AAGTGGACTTTTGCATATGC+TGG - chr8_4:2235326-2235345 Msa1307050:intron 40.0%
!! AAAGAAAGCGCCTAATAGCC+CGG + chr8_4:2233855-2233874 None:intergenic 45.0%
!! GAGGAGTATCCGAGTATGAT+TGG - chr8_4:2234301-2234320 Msa1307050:three_prime_UTR 45.0%
GGAATAGGAAGTGGCATAAC+AGG + chr8_4:2234428-2234447 None:intergenic 45.0%
! GAATCTGGAGTAGAGCATGT+GGG + chr8_4:2234499-2234518 None:intergenic 45.0%
! AGAATCTGGAGTAGAGCATG+TGG + chr8_4:2234500-2234519 None:intergenic 45.0%
GATTCGGATGAGGTAGAATC+TGG + chr8_4:2234514-2234533 None:intergenic 45.0%
TTAGGACCAAGATTCGGATG+AGG + chr8_4:2234524-2234543 None:intergenic 45.0%
!! GGTTGTTGGCTTGACGTATT+AGG + chr8_4:2234542-2234561 None:intergenic 45.0%
! TGCCCAAGGTGTTATCTCAT+TGG - chr8_4:2234598-2234617 Msa1307050:intron 45.0%
CCACAAGTTGTGCATTGTCT+AGG + chr8_4:2234820-2234839 None:intergenic 45.0%
CCTAGACAATGCACAACTTG+TGG - chr8_4:2234817-2234836 Msa1307050:CDS 45.0%
ACTCACAAACTACTTGCGAG+AGG + chr8_4:2234932-2234951 None:intergenic 45.0%
AGTTTGTGAGTGAGACGATG+TGG - chr8_4:2234941-2234960 Msa1307050:intron 45.0%
AGTGAGACGATGTGGCTAAA+AGG - chr8_4:2234949-2234968 Msa1307050:intron 45.0%
! GCTAAAAGGTGCAGAGACAT+TGG - chr8_4:2234963-2234982 Msa1307050:intron 45.0%
TTGGGAGTTAAGTCAACTCC+AGG - chr8_4:2234982-2235001 Msa1307050:intron 45.0%
ACAGTGATGAAACGGAGGAT+TGG - chr8_4:2235115-2235134 Msa1307050:intron 45.0%
GCGCCTAATGGAATAACAAC+AGG - chr8_4:2235165-2235184 Msa1307050:intron 45.0%
! TACCCCGTGTGAAGATTTTG+CGG - chr8_4:2235242-2235261 Msa1307050:intron 45.0%
TACCGCAAAATCTTCACACG+GGG + chr8_4:2235247-2235266 None:intergenic 45.0%
CATATGCTGGTGTTTCAAGC+CGG - chr8_4:2235339-2235358 Msa1307050:intron 45.0%
!! AAAGAAAGCGCCTAATAGCC+CGG + chr8_4:2233855-2233874 None:intergenic 45.0%
!! GAGGAGTATCCGAGTATGAT+TGG - chr8_4:2234301-2234320 Msa1307050:three_prime_UTR 45.0%
GGAATAGGAAGTGGCATAAC+AGG + chr8_4:2234428-2234447 None:intergenic 45.0%
! GAATCTGGAGTAGAGCATGT+GGG + chr8_4:2234499-2234518 None:intergenic 45.0%
! AGAATCTGGAGTAGAGCATG+TGG + chr8_4:2234500-2234519 None:intergenic 45.0%
GATTCGGATGAGGTAGAATC+TGG + chr8_4:2234514-2234533 None:intergenic 45.0%
TTAGGACCAAGATTCGGATG+AGG + chr8_4:2234524-2234543 None:intergenic 45.0%
!! GGTTGTTGGCTTGACGTATT+AGG + chr8_4:2234542-2234561 None:intergenic 45.0%
! TGCCCAAGGTGTTATCTCAT+TGG - chr8_4:2234598-2234617 Msa1307050:intron 45.0%
CCACAAGTTGTGCATTGTCT+AGG + chr8_4:2234820-2234839 None:intergenic 45.0%
CCTAGACAATGCACAACTTG+TGG - chr8_4:2234817-2234836 Msa1307050:CDS 45.0%
ACTCACAAACTACTTGCGAG+AGG + chr8_4:2234932-2234951 None:intergenic 45.0%
AGTTTGTGAGTGAGACGATG+TGG - chr8_4:2234941-2234960 Msa1307050:intron 45.0%
AGTGAGACGATGTGGCTAAA+AGG - chr8_4:2234949-2234968 Msa1307050:intron 45.0%
! GCTAAAAGGTGCAGAGACAT+TGG - chr8_4:2234963-2234982 Msa1307050:intron 45.0%
TTGGGAGTTAAGTCAACTCC+AGG - chr8_4:2234982-2235001 Msa1307050:intron 45.0%
ACAGTGATGAAACGGAGGAT+TGG - chr8_4:2235115-2235134 Msa1307050:intron 45.0%
GCGCCTAATGGAATAACAAC+AGG - chr8_4:2235165-2235184 Msa1307050:intron 45.0%
! TACCCCGTGTGAAGATTTTG+CGG - chr8_4:2235242-2235261 Msa1307050:intron 45.0%
TACCGCAAAATCTTCACACG+GGG + chr8_4:2235247-2235266 None:intergenic 45.0%
CATATGCTGGTGTTTCAAGC+CGG - chr8_4:2235339-2235358 Msa1307050:intron 45.0%
!! GTCTTTGGAACCGGGCTATT+AGG - chr8_4:2233842-2233861 Msa1307050:three_prime_UTR 50.0%
AACAGGTCGTGCATTTGGCA+GGG + chr8_4:2234411-2234430 None:intergenic 50.0%
TAACAGGTCGTGCATTTGGC+AGG + chr8_4:2234412-2234431 None:intergenic 50.0%
GGCATAACAGGTCGTGCATT+TGG + chr8_4:2234416-2234435 None:intergenic 50.0%
GTGACTGCTGTGCTCATCTT+TGG + chr8_4:2234857-2234876 None:intergenic 50.0%
AGATGAGCACAGCAGTCACA+TGG - chr8_4:2234858-2234877 Msa1307050:intron 50.0%
AGCACAGCAGTCACATGGAT+TGG - chr8_4:2234863-2234882 Msa1307050:intron 50.0%
GTCTCGGTTGGCAAAAAACC+TGG + chr8_4:2235003-2235022 None:intergenic 50.0%
! TTTTCTTCGGTGGTCTCGGT+TGG + chr8_4:2235015-2235034 None:intergenic 50.0%
ACGGAGGATTGGATATGCAG+AGG - chr8_4:2235126-2235145 Msa1307050:intron 50.0%
! ATGCAGGGCACCTATGAAGA+AGG - chr8_4:2235392-2235411 Msa1307050:CDS 50.0%
!! GTCTTTGGAACCGGGCTATT+AGG - chr8_4:2233842-2233861 Msa1307050:three_prime_UTR 50.0%
AACAGGTCGTGCATTTGGCA+GGG + chr8_4:2234411-2234430 None:intergenic 50.0%
TAACAGGTCGTGCATTTGGC+AGG + chr8_4:2234412-2234431 None:intergenic 50.0%
GGCATAACAGGTCGTGCATT+TGG + chr8_4:2234416-2234435 None:intergenic 50.0%
GTGACTGCTGTGCTCATCTT+TGG + chr8_4:2234857-2234876 None:intergenic 50.0%
AGATGAGCACAGCAGTCACA+TGG - chr8_4:2234858-2234877 Msa1307050:intron 50.0%
AGCACAGCAGTCACATGGAT+TGG - chr8_4:2234863-2234882 Msa1307050:intron 50.0%
GTCTCGGTTGGCAAAAAACC+TGG + chr8_4:2235003-2235022 None:intergenic 50.0%
! TTTTCTTCGGTGGTCTCGGT+TGG + chr8_4:2235015-2235034 None:intergenic 50.0%
ACGGAGGATTGGATATGCAG+AGG - chr8_4:2235126-2235145 Msa1307050:intron 50.0%
! ATGCAGGGCACCTATGAAGA+AGG - chr8_4:2235392-2235411 Msa1307050:CDS 50.0%
ACAGGTCGTGCATTTGGCAG+GGG + chr8_4:2234410-2234429 None:intergenic 55.0%
TTCCGACCTTTACCTCCAGC+TGG - chr8_4:2234473-2234492 Msa1307050:CDS 55.0%
GTTGGCAGTTCCGTTCGATG+AGG - chr8_4:2235047-2235066 Msa1307050:intron 55.0%
ACAGGTCGTGCATTTGGCAG+GGG + chr8_4:2234410-2234429 None:intergenic 55.0%
TTCCGACCTTTACCTCCAGC+TGG - chr8_4:2234473-2234492 Msa1307050:CDS 55.0%
GTTGGCAGTTCCGTTCGATG+AGG - chr8_4:2235047-2235066 Msa1307050:intron 55.0%
CAGGTCGTGCATTTGGCAGG+GGG + chr8_4:2234409-2234428 None:intergenic 60.0%
GGCCAGCTGGAGGTAAAGGT+CGG + chr8_4:2234478-2234497 None:intergenic 60.0%
TGTGGGCCAGCTGGAGGTAA+AGG + chr8_4:2234482-2234501 None:intergenic 60.0%
AGTAGAGCATGTGGGCCAGC+TGG + chr8_4:2234491-2234510 None:intergenic 60.0%
GGCTAATCAAGCCCCTGCGA+AGG - chr8_4:2234619-2234638 Msa1307050:intron 60.0%
CAGGTCGTGCATTTGGCAGG+GGG + chr8_4:2234409-2234428 None:intergenic 60.0%
GGCCAGCTGGAGGTAAAGGT+CGG + chr8_4:2234478-2234497 None:intergenic 60.0%
TGTGGGCCAGCTGGAGGTAA+AGG + chr8_4:2234482-2234501 None:intergenic 60.0%
AGTAGAGCATGTGGGCCAGC+TGG + chr8_4:2234491-2234510 None:intergenic 60.0%
GGCTAATCAAGCCCCTGCGA+AGG - chr8_4:2234619-2234638 Msa1307050:intron 60.0%
AGAGCATGTGGGCCAGCTGG+AGG + chr8_4:2234488-2234507 None:intergenic 65.0%
AGAGCATGTGGGCCAGCTGG+AGG + chr8_4:2234488-2234507 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 2233827 2235436 2233827 ID=Msa1307050;Name=Msa1307050
chr8_4 mRNA 2233827 2235436 2233827 ID=Msa1307050-mRNA-1;Parent=Msa1307050;Name=Msa1307050-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.66|0.5|0.75|0.66|0.25|4|372|175
chr8_4 exon 2233827 2233865 2233827 ID=Msa1307050-mRNA-1:exon:734;Parent=Msa1307050-mRNA-1
chr8_4 exon 2233988 2234527 2233988 ID=Msa1307050-mRNA-1:exon:733;Parent=Msa1307050-mRNA-1
chr8_4 exon 2234623 2234862 2234623 ID=Msa1307050-mRNA-1:exon:732;Parent=Msa1307050-mRNA-1
chr8_4 exon 2235356 2235436 2235356 ID=Msa1307050-mRNA-1:exon:731;Parent=Msa1307050-mRNA-1
chr8_4 CDS 2235356 2235436 2235356 ID=Msa1307050-mRNA-1:cds;Parent=Msa1307050-mRNA-1
chr8_4 CDS 2234623 2234862 2234623 ID=Msa1307050-mRNA-1:cds;Parent=Msa1307050-mRNA-1
chr8_4 CDS 2234321 2234527 2234321 ID=Msa1307050-mRNA-1:cds;Parent=Msa1307050-mRNA-1
chr8_4 three_prime_UTR 2233988 2234320 2233988 ID=Msa1307050-mRNA-1:three_prime_utr;Parent=Msa1307050-mRNA-1
chr8_4 three_prime_UTR 2233827 2233865 2233827 ID=Msa1307050-mRNA-1:three_prime_utr;Parent=Msa1307050-mRNA-1
Gene Sequence

>Msa1307050

ATCGCATATATAGGTGTCTTTGGAACCGGGCTATTAGGCGCTTTCTTTTCCAATTTTTTGTTCAAGAAACATCATTTCCAATTTGGCCCCCTGCCAAATGCACGACCTGTTATGCCACTTCCTATTCCATACCAAAGAATCGGAAACAACTCTTTCCGACCTTTACCTCCAGCTGGCCCACATGCTCTACTCCAGATTCTACCTCATCCGAATCTTGGTCCTAATACGTCAAGCCAACAACCAATTGTTGGATATTCTAATTTGATTAGTTCACTCATTGCCCAAGGTGTTATCTCATTGGCTAATCAAGCCCCTGCGAAGGATTTTATTGGCCTTGAGTTTGATCCAAATATTCTCAAGGTTCGTCATGAATATGTAATTTGTGATTTATATGGTGATTTTCCTAGACAATGCACAACTTGTGGCCATCGATTCAAATCCAAAGATGAGCACAGCAGTCACATGGATTGGCATGTGACAAAGAACCGTATGTCTAAAAACTGTAAGCAAAAGTCCTCTCGCAAGTAG
Protein sequence

>Msa1307050

IAYIGVFGTGLLGAFFSNFLFKKHHFQFGPLPNARPVMPLPIPYQRIGNNSFRPLPPAGPHALLQILPHPNLGPNTSSQQPIVGYSNLISSLIAQGVISLANQAPAKDFIGLEFDPNILKVRHEYVICDLYGDFPRQCTTCGHRFKSKDEHSSHMDWHVTKNRMSKNCKQKSSRK*