AlfalfaGEDB | Msa1308020

M. sativa cultivar zhongmu-4 / Msa1308020

KEGG: -
GO: GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308020	sp\|Q9LY00\|WRK70_ARATH	38.674	181	88	6	6	173	10	180	3.38e-31	121

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308020	G7LAR2	81.333	300	31	6	5	302	3	279	2.44e-160	457

TFs/TRs:

Gene ID	Type	Classification
Msa1308020	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308020	MtrunA17_Chr8g0334541	81.333	300	31	6	5	302	3	279	4.69e-164	457
Msa1308020	MtrunA17_Chr8g0350621	50.189	265	98	9	1	253	1	243	4.69e-71	220
Msa1308020	MtrunA17_Chr7g0244981	41.156	294	136	7	4	287	11	277	4.11e-65	206
Msa1308020	MtrunA17_Chr7g0244971	44.710	293	137	9	4	287	12	288	1.01e-64	205
Msa1308020	MtrunA17_Chr2g0315371	41.096	219	105	5	9	205	18	234	1.55e-46	158
Msa1308020	MtrunA17_Chr2g0315381	39.024	246	113	7	2	214	10	251	1.21e-40	143
Msa1308020	MtrunA17_Chr8g0350631	49.432	176	49	7	134	300	5	149	1.96e-38	133
Msa1308020	MtrunA17_Chr2g0315361	41.597	238	107	10	6	213	14	249	1.49e-37	135
Msa1308020	MtrunA17_Chr5g0428351	34.498	229	112	4	8	236	92	282	7.63e-34	125
Msa1308020	MtrunA17_Chr3g0128671	44.379	169	80	6	13	172	23	186	1.38e-31	119
Msa1308020	MtrunA17_Chr7g0275531	37.288	177	95	5	4	172	9	177	1.49e-26	106
Msa1308020	MtrunA17_Chr3g0126391	30.288	208	113	3	1	182	1	202	9.45e-24	99.0
Msa1308020	MtrunA17_Chr8g0350641	44.526	137	63	5	6	133	7	139	1.08e-23	94.4
Msa1308020	MtrunA17_Chr8g0387981	31.795	195	105	4	8	181	13	200	4.90e-23	97.4
Msa1308020	MtrunA17_Chr8g0347251	46.667	90	41	1	94	176	110	199	4.08e-20	89.0
Msa1308020	MtrunA17_Chr7g0224801	54.237	59	24	1	113	171	93	148	1.34e-14	70.5
Msa1308020	MtrunA17_Chr8g0383351	41.333	75	41	1	113	187	160	231	1.39e-13	68.9
Msa1308020	MtrunA17_Chr7g0238921	56.897	58	22	1	113	170	124	178	4.98e-13	68.6
Msa1308020	MtrunA17_Chr3g0129361	47.458	59	28	1	113	171	144	199	9.48e-13	66.2
Msa1308020	MtrunA17_Chr2g0315401	50.794	63	21	2	141	198	19	76	1.63e-12	63.9
Msa1308020	MtrunA17_Chr5g0418781	50.000	58	26	1	113	170	124	178	1.66e-12	66.6
Msa1308020	MtrunA17_Chr8g0348311	43.590	78	40	2	96	171	136	211	2.50e-12	66.6
Msa1308020	MtrunA17_Chr4g0051881	51.724	58	25	1	113	170	178	232	2.98e-12	66.2
Msa1308020	MtrunA17_Chr5g0443131	50.000	58	26	1	113	170	157	211	3.51e-12	65.9
Msa1308020	MtrunA17_Chr8g0335811	50.000	58	26	1	113	170	154	208	3.84e-12	64.7
Msa1308020	MtrunA17_Chr7g0248721	51.562	64	25	3	113	174	141	200	4.16e-12	64.3
Msa1308020	MtrunA17_Chr5g0431811	37.500	96	52	3	111	202	670	761	8.06e-12	65.9
Msa1308020	MtrunA17_Chr1g0194481	47.458	59	28	1	113	171	158	213	2.11e-11	63.5
Msa1308020	MtrunA17_Chr8g0386241	36.047	86	50	2	85	170	77	157	2.75e-11	61.6
Msa1308020	MtrunA17_Chr2g0294231	47.458	59	28	1	113	171	203	258	3.14e-11	63.5
Msa1308020	MtrunA17_Chr4g0068541	44.828	58	29	1	113	170	208	262	5.79e-11	62.8
Msa1308020	MtrunA17_Chr7g0270191	47.458	59	28	1	113	171	145	200	5.98e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308020	AT3G56400.1	38.674	181	88	6	6	173	10	180	3.44e-32	121
Msa1308020	AT2G46400.1	34.536	194	111	5	7	200	8	185	4.98e-26	104
Msa1308020	AT1G66550.1	32.524	206	109	7	6	203	8	191	6.22e-26	103
Msa1308020	AT1G66600.1	33.846	195	108	4	6	199	8	182	1.43e-25	102
Msa1308020	AT1G66560.1	35.533	197	100	6	7	199	9	182	2.46e-25	102
Msa1308020	AT1G66550.2	32.057	209	101	6	6	203	8	186	5.07e-25	101
Msa1308020	AT4G11070.1	30.964	197	112	2	3	175	7	203	9.66e-24	99.0
Msa1308020	AT5G22570.1	32.512	203	115	6	5	197	8	198	1.98e-23	97.8
Msa1308020	AT2G40750.1	29.060	234	130	6	6	207	14	243	4.10e-22	95.1
Msa1308020	AT5G01900.1	36.416	173	98	5	4	172	5	169	1.04e-21	92.4
Msa1308020	AT4G11070.2	32.432	185	93	3	3	175	7	171	5.19e-20	88.2
Msa1308020	AT1G80590.1	36.800	125	70	3	82	201	49	169	3.57e-19	85.1
Msa1308020	AT2G40740.1	44.792	96	40	2	90	172	137	232	4.58e-19	85.9
Msa1308020	AT5G24110.1	32.000	175	101	4	4	174	14	174	4.70e-19	85.9
Msa1308020	AT2G40740.3	44.792	96	40	2	90	172	146	241	6.05e-19	85.5
Msa1308020	AT4G23810.1	46.591	88	40	1	94	174	132	219	2.47e-18	84.3
Msa1308020	AT1G64000.1	45.946	74	32	3	113	184	114	181	2.18e-13	68.2
Msa1308020	AT2G46130.1	51.724	58	25	1	113	170	30	84	2.43e-13	65.9
Msa1308020	AT1G69310.4	50.000	58	26	1	113	170	147	201	8.16e-13	67.8
Msa1308020	AT1G69310.2	50.000	58	26	1	113	170	147	201	8.16e-13	67.8
Msa1308020	AT1G69310.1	50.000	58	26	1	113	170	147	201	8.16e-13	67.8
Msa1308020	AT5G45260.1	48.485	66	32	1	107	170	1200	1265	1.13e-12	68.9
Msa1308020	AT5G49520.1	50.847	59	26	1	113	171	221	276	1.38e-12	68.2
Msa1308020	AT5G41570.1	46.875	64	29	2	113	174	98	158	3.00e-12	64.7
Msa1308020	AT2G44745.1	50.000	58	26	1	113	170	145	199	3.02e-12	65.5
Msa1308020	AT5G46350.1	40.909	88	44	3	113	195	183	267	3.41e-12	66.6
Msa1308020	AT4G39410.1	46.552	58	28	1	113	170	223	277	3.50e-12	66.2
Msa1308020	AT4G18170.1	41.667	96	49	3	77	170	136	226	4.73e-12	65.9
Msa1308020	AT1G29860.1	39.326	89	46	2	113	196	136	221	4.85e-12	65.5
Msa1308020	AT5G13080.1	49.153	59	27	1	113	171	67	122	9.88e-12	62.4
Msa1308020	AT4G01250.1	32.258	124	81	2	47	170	63	183	1.04e-11	64.7
Msa1308020	AT3G62340.1	37.755	98	46	3	113	198	118	212	5.20e-11	62.4

Find 75 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATATACACACCTTGAGTATT+TGG	0.198242	8_4:-4163220	None:intergenic
GAAATAGATTTATCTAAAAT+TGG	0.218405	8_4:-4163725	None:intergenic
AAAACGAGTAACCTCATTAT+TGG	0.242421	8_4:+4163951	Msa1308020:three_prime_UTR
TGATGTCGACGATGATAAAA+AGG	0.250505	8_4:-4163615	None:intergenic
TCTGCACTTGTTTGGTTGCT+TGG	0.276455	8_4:-4163427	None:intergenic
ACAAGATGTTGGGCAAATAA+TGG	0.285380	8_4:-4162620	None:intergenic
AAGTGAGAGAGTTGAAAATA+TGG	0.295847	8_4:+4162490	Msa1308020:exon
CTCATGTACAATATACAAAA+TGG	0.325930	8_4:-4163917	None:intergenic
TTGAATTCTCTGCACTTGTT+TGG	0.343489	8_4:-4163435	None:intergenic
CAAGATGTTGGGCAAATAAT+GGG	0.348277	8_4:-4162619	None:intergenic
CAAGGAGAACTCACCTTCCT+TGG	0.368416	8_4:+4163679	Msa1308020:CDS
TTTGATGATGTTTGTTCAAA+AGG	0.368943	8_4:-4162679	None:intergenic
GATTTATCTAAAATTGGTTC+AGG	0.379960	8_4:-4163719	None:intergenic
ACAAGTGTGGTGGCCGTAAT+AGG	0.383562	8_4:-4163483	None:intergenic
ACCTTGAGTATTTGGAATTG+AGG	0.393271	8_4:-4163212	None:intergenic
TATAATTGAAAGCCATGAAA+AGG	0.416194	8_4:-4163875	None:intergenic
AAACGAGTAACCTCATTATT+GGG	0.421058	8_4:+4163952	Msa1308020:three_prime_UTR
CAACAGATGATGGGCATGAA+TGG	0.421200	8_4:+4163166	Msa1308020:CDS
CAATCTTGTTCGGTTGGAGT+TGG	0.425942	8_4:-4163590	None:intergenic
TTGTATAAGACCACCTATTA+CGG	0.432341	8_4:+4163470	Msa1308020:CDS
AACACACATACAAGAGCATT+AGG	0.440774	8_4:+4162365	Msa1308020:five_prime_UTR
GGGCATGAATGGAGAAAGTA+TGG	0.453906	8_4:+4163177	Msa1308020:CDS
AACGTTGCTTACAAGATGTT+GGG	0.454457	8_4:-4162630	None:intergenic
GCATTAGGAAGATGGAAGGA+AGG	0.455060	8_4:+4162380	Msa1308020:five_prime_UTR
GTTGTTAAAGCTAAGCAATA+TGG	0.457680	8_4:-4163561	None:intergenic
TCAGAAAGACCAACAGATGA+TGG	0.460980	8_4:+4163156	Msa1308020:CDS
CAACGTTGCTTACAAGATGT+TGG	0.464878	8_4:-4162631	None:intergenic
AATGATGATATATGAGGTAA+TGG	0.466440	8_4:+4164003	Msa1308020:three_prime_UTR
CACTTGAATCTGATGATCAC+AGG	0.481025	8_4:+4163786	Msa1308020:CDS
TAATATAATGATGATATATG+AGG	0.494030	8_4:+4163997	Msa1308020:three_prime_UTR
TTTGAACAAACATCATCAAA+GGG	0.495098	8_4:+4162682	Msa1308020:CDS
TTTAACTATTAAATCTGAAG+AGG	0.496351	8_4:+4162718	Msa1308020:CDS
CATTCATGCCCATCATCTGT+TGG	0.504398	8_4:-4163165	None:intergenic
TGATAGAGATGAGGCAATGA+AGG	0.505201	8_4:+4162514	Msa1308020:CDS
AAGGGACAATCTTGTTCGGT+TGG	0.505479	8_4:-4163596	None:intergenic
AGTATTATCATGAGGAGAAG+TGG	0.509303	8_4:-4163531	None:intergenic
ATAATTGAAAGCCATGAAAA+GGG	0.510799	8_4:-4163874	None:intergenic
TATACAAAATGGACATGTAA+AGG	0.515172	8_4:-4163906	None:intergenic
GATATGATGATAATATTGTA+TGG	0.516217	8_4:+4163809	Msa1308020:CDS
CATTAGGAAGATGGAAGGAA+GGG	0.523179	8_4:+4162381	Msa1308020:five_prime_UTR
ATACAAGAGCATTAGGAAGA+TGG	0.532214	8_4:+4162372	Msa1308020:five_prime_UTR
CTAGAGGATCTCACAAAAGA+AGG	0.538260	8_4:+4162780	Msa1308020:CDS
GATGTTTGTTCAAAAGGAAG+AGG	0.548943	8_4:-4162673	None:intergenic
GTGAGATCCTCTAGCACCAC+GGG	0.549156	8_4:-4162771	None:intergenic
ATATATGCAGGGACTACTAC+AGG	0.552760	8_4:+4163378	Msa1308020:intron
TAATAGGTGGTCTTATACAA+GGG	0.559513	8_4:-4163467	None:intergenic
TAGATAAATCTATTTCTACA+AGG	0.566016	8_4:+4163732	Msa1308020:CDS
TCCTCAATTCCAAATACTCA+AGG	0.566315	8_4:+4163211	Msa1308020:CDS
GTATTATCATGAGGAGAAGT+GGG	0.567061	8_4:-4163530	None:intergenic
CAGAAAGACCAACAGATGAT+GGG	0.573763	8_4:+4163157	Msa1308020:CDS
ATGTTTGTTCAAAAGGAAGA+GGG	0.576811	8_4:-4162672	None:intergenic
GTAATAGGTGGTCTTATACA+AGG	0.578341	8_4:-4163468	None:intergenic
AGTTCCACCCGTGGTGCTAG+AGG	0.580528	8_4:+4162764	Msa1308020:CDS
AGAGAGCAGGTATTCATCCA+AGG	0.581141	8_4:-4163696	None:intergenic
GAAAGCCATGAAAAGGGTGA+AGG	0.584427	8_4:-4163868	None:intergenic
CATGCATTGTCCCAATAATG+AGG	0.585488	8_4:-4163962	None:intergenic
AAGAGCATTAGGAAGATGGA+AGG	0.586002	8_4:+4162376	Msa1308020:five_prime_UTR
GACAATGCATGTGATGACTT+TGG	0.586647	8_4:+4163974	Msa1308020:three_prime_UTR
GCAATATTCCACTGAAAGTG+AGG	0.588997	8_4:-4163657	None:intergenic
ACTTTCAGTGGAATATTGCA+AGG	0.591517	8_4:+4163661	Msa1308020:CDS
AGCAGGTATTCATCCAAGGA+AGG	0.605278	8_4:-4163692	None:intergenic
GTTAAAGCTAAGCAATATGG+AGG	0.618068	8_4:-4163558	None:intergenic
TATTGTCTTTGTTACAAGTG+TGG	0.619139	8_4:-4163496	None:intergenic
TGTCTTTGTTACAAGTGTGG+TGG	0.626053	8_4:-4163493	None:intergenic
GAGGAAGTAGTATTATCATG+AGG	0.627255	8_4:-4163539	None:intergenic
AAATTGGTTCAGGAGAGAGC+AGG	0.628355	8_4:-4163709	None:intergenic
GCAATGAAGGAGCTTGTGAG+AGG	0.633274	8_4:+4162527	Msa1308020:CDS
CAATGAAGGAGCTTGTGAGA+GGG	0.635141	8_4:+4162528	Msa1308020:CDS
TGTGAGATCCTCTAGCACCA+CGG	0.642464	8_4:-4162772	None:intergenic
AGATCCTCTAGCACCACGGG+TGG	0.645633	8_4:-4162768	None:intergenic
AGTGTGGTGGCCGTAATAGG+TGG	0.645916	8_4:-4163480	None:intergenic
AAATATGGATGATAGAGATG+AGG	0.650081	8_4:+4162505	Msa1308020:exon
ACTTGAATCTGATGATCACA+GGG	0.651483	8_4:+4163787	Msa1308020:CDS
TAGGTGGTCTTATACAAGGG+AGG	0.682870	8_4:-4163464	None:intergenic
TACAAGACTAGTTCCACCCG+TGG	0.693459	8_4:+4162755	Msa1308020:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATATATATTAATAATATAT+TGG	-	chr8_4:4162849-4162868	None:intergenic	0.0%
!!	ATTATTAATATATATATATA+TGG	+	chr8_4:4162853-4162872	Msa1308020:intron	0.0%
!!	TATATATATTAATAATATAT+TGG	-	chr8_4:4162849-4162868	None:intergenic	0.0%
!!	ATTATTAATATATATATATA+TGG	+	chr8_4:4162853-4162872	Msa1308020:intron	0.0%
!!	CATATATTAATTCAAATCAT+TGG	-	chr8_4:4163365-4163384	None:intergenic	15.0%
!!!	GAAATAGATTTATCTAAAAT+TGG	-	chr8_4:4163728-4163747	None:intergenic	15.0%
!!	TAATATAATGATGATATATG+AGG	+	chr8_4:4163997-4164016	Msa1308020:three_prime_UTR	15.0%
!!	CATATATTAATTCAAATCAT+TGG	-	chr8_4:4163365-4163384	None:intergenic	15.0%
!!!	GAAATAGATTTATCTAAAAT+TGG	-	chr8_4:4163728-4163747	None:intergenic	15.0%
!!	TAATATAATGATGATATATG+AGG	+	chr8_4:4163997-4164016	Msa1308020:three_prime_UTR	15.0%
!!	TTTAACTATTAAATCTGAAG+AGG	+	chr8_4:4162718-4162737	Msa1308020:CDS	20.0%
!!	ACAAACTGTAAAAAGTTATT+AGG	-	chr8_4:4163324-4163343	None:intergenic	20.0%
!!	CAAATCATTGGATATATATT+AGG	-	chr8_4:4163353-4163372	None:intergenic	20.0%
!!	TGATTTGAATTAATATATGC+AGG	+	chr8_4:4163366-4163385	Msa1308020:intron	20.0%
!!	GATTTGAATTAATATATGCA+GGG	+	chr8_4:4163367-4163386	Msa1308020:intron	20.0%
!!!	TAGATAAATCTATTTCTACA+AGG	+	chr8_4:4163732-4163751	Msa1308020:CDS	20.0%
!!	GATATGATGATAATATTGTA+TGG	+	chr8_4:4163809-4163828	Msa1308020:CDS	20.0%
!!	TTTAACTATTAAATCTGAAG+AGG	+	chr8_4:4162718-4162737	Msa1308020:CDS	20.0%
!!	ACAAACTGTAAAAAGTTATT+AGG	-	chr8_4:4163324-4163343	None:intergenic	20.0%
!!	CAAATCATTGGATATATATT+AGG	-	chr8_4:4163353-4163372	None:intergenic	20.0%
!!	TGATTTGAATTAATATATGC+AGG	+	chr8_4:4163366-4163385	Msa1308020:intron	20.0%
!!	GATTTGAATTAATATATGCA+GGG	+	chr8_4:4163367-4163386	Msa1308020:intron	20.0%
!!!	TAGATAAATCTATTTCTACA+AGG	+	chr8_4:4163732-4163751	Msa1308020:CDS	20.0%
!!	GATATGATGATAATATTGTA+TGG	+	chr8_4:4163809-4163828	Msa1308020:CDS	20.0%
!!!	TTTGATGATGTTTGTTCAAA+AGG	-	chr8_4:4162682-4162701	None:intergenic	25.0%
!!	TTTTGAACAAACATCATCAA+AGG	+	chr8_4:4162681-4162700	Msa1308020:CDS	25.0%
!	TTTGAACAAACATCATCAAA+GGG	+	chr8_4:4162682-4162701	Msa1308020:CDS	25.0%
!!!	AAGTAGTTTGAGTTTTAACA+AGG	-	chr8_4:4162960-4162979	None:intergenic	25.0%
!!	GAACATTTTCTATAGAATCA+TGG	+	chr8_4:4163038-4163057	Msa1308020:intron	25.0%
!!	TAGAATCATGGTTAAGTTTT+TGG	+	chr8_4:4163050-4163069	Msa1308020:intron	25.0%
!!!	TAACTTTTTACAGTTTGTTC+AGG	+	chr8_4:4163326-4163345	Msa1308020:intron	25.0%
!!!	AACTTTTTACAGTTTGTTCA+GGG	+	chr8_4:4163327-4163346	Msa1308020:intron	25.0%
!!	GATTTATCTAAAATTGGTTC+AGG	-	chr8_4:4163722-4163741	None:intergenic	25.0%
!	ATAATTGAAAGCCATGAAAA+GGG	-	chr8_4:4163877-4163896	None:intergenic	25.0%
!	TATAATTGAAAGCCATGAAA+AGG	-	chr8_4:4163878-4163897	None:intergenic	25.0%
!	TATACAAAATGGACATGTAA+AGG	-	chr8_4:4163909-4163928	None:intergenic	25.0%
!	CTCATGTACAATATACAAAA+TGG	-	chr8_4:4163920-4163939	None:intergenic	25.0%
!	AATGATGATATATGAGGTAA+TGG	+	chr8_4:4164003-4164022	Msa1308020:three_prime_UTR	25.0%
!!!	TTTGATGATGTTTGTTCAAA+AGG	-	chr8_4:4162682-4162701	None:intergenic	25.0%
!!	TTTTGAACAAACATCATCAA+AGG	+	chr8_4:4162681-4162700	Msa1308020:CDS	25.0%
!	TTTGAACAAACATCATCAAA+GGG	+	chr8_4:4162682-4162701	Msa1308020:CDS	25.0%
!!!	AAGTAGTTTGAGTTTTAACA+AGG	-	chr8_4:4162960-4162979	None:intergenic	25.0%
!!	GAACATTTTCTATAGAATCA+TGG	+	chr8_4:4163038-4163057	Msa1308020:intron	25.0%
!!	TAGAATCATGGTTAAGTTTT+TGG	+	chr8_4:4163050-4163069	Msa1308020:intron	25.0%
!!!	TAACTTTTTACAGTTTGTTC+AGG	+	chr8_4:4163326-4163345	Msa1308020:intron	25.0%
!!!	AACTTTTTACAGTTTGTTCA+GGG	+	chr8_4:4163327-4163346	Msa1308020:intron	25.0%
!!	GATTTATCTAAAATTGGTTC+AGG	-	chr8_4:4163722-4163741	None:intergenic	25.0%
!	ATAATTGAAAGCCATGAAAA+GGG	-	chr8_4:4163877-4163896	None:intergenic	25.0%
!	TATAATTGAAAGCCATGAAA+AGG	-	chr8_4:4163878-4163897	None:intergenic	25.0%
!	TATACAAAATGGACATGTAA+AGG	-	chr8_4:4163909-4163928	None:intergenic	25.0%
!	CTCATGTACAATATACAAAA+TGG	-	chr8_4:4163920-4163939	None:intergenic	25.0%
!	AATGATGATATATGAGGTAA+TGG	+	chr8_4:4164003-4164022	Msa1308020:three_prime_UTR	25.0%
	AAGTGAGAGAGTTGAAAATA+TGG	+	chr8_4:4162490-4162509	Msa1308020:exon	30.0%
	AAATATGGATGATAGAGATG+AGG	+	chr8_4:4162505-4162524	Msa1308020:exon	30.0%
	ATGTTTGTTCAAAAGGAAGA+GGG	-	chr8_4:4162675-4162694	None:intergenic	30.0%
	AACATGAAATTCTCATTGTG+TGG	+	chr8_4:4162925-4162944	Msa1308020:intron	30.0%
	ATATACACACCTTGAGTATT+TGG	-	chr8_4:4163223-4163242	None:intergenic	30.0%
!!	TAATAGGTGGTCTTATACAA+GGG	-	chr8_4:4163470-4163489	None:intergenic	30.0%
	TTGTATAAGACCACCTATTA+CGG	+	chr8_4:4163470-4163489	Msa1308020:CDS	30.0%
	TATTGTCTTTGTTACAAGTG+TGG	-	chr8_4:4163499-4163518	None:intergenic	30.0%
	GTTGTTAAAGCTAAGCAATA+TGG	-	chr8_4:4163564-4163583	None:intergenic	30.0%
	TAAAAAGGGACAATCTTGTT+CGG	-	chr8_4:4163603-4163622	None:intergenic	30.0%
!!	TTTTAATTCCTCACTTTCAG+TGG	+	chr8_4:4163649-4163668	Msa1308020:CDS	30.0%
	AAAACGAGTAACCTCATTAT+TGG	+	chr8_4:4163951-4163970	Msa1308020:three_prime_UTR	30.0%
	AAACGAGTAACCTCATTATT+GGG	+	chr8_4:4163952-4163971	Msa1308020:three_prime_UTR	30.0%
	AAGTGAGAGAGTTGAAAATA+TGG	+	chr8_4:4162490-4162509	Msa1308020:exon	30.0%
	AAATATGGATGATAGAGATG+AGG	+	chr8_4:4162505-4162524	Msa1308020:exon	30.0%
	ATGTTTGTTCAAAAGGAAGA+GGG	-	chr8_4:4162675-4162694	None:intergenic	30.0%
	AACATGAAATTCTCATTGTG+TGG	+	chr8_4:4162925-4162944	Msa1308020:intron	30.0%
	ATATACACACCTTGAGTATT+TGG	-	chr8_4:4163223-4163242	None:intergenic	30.0%
!!	TAATAGGTGGTCTTATACAA+GGG	-	chr8_4:4163470-4163489	None:intergenic	30.0%
	TTGTATAAGACCACCTATTA+CGG	+	chr8_4:4163470-4163489	Msa1308020:CDS	30.0%
	TATTGTCTTTGTTACAAGTG+TGG	-	chr8_4:4163499-4163518	None:intergenic	30.0%
	GTTGTTAAAGCTAAGCAATA+TGG	-	chr8_4:4163564-4163583	None:intergenic	30.0%
	TAAAAAGGGACAATCTTGTT+CGG	-	chr8_4:4163603-4163622	None:intergenic	30.0%
!!	TTTTAATTCCTCACTTTCAG+TGG	+	chr8_4:4163649-4163668	Msa1308020:CDS	30.0%
	AAAACGAGTAACCTCATTAT+TGG	+	chr8_4:4163951-4163970	Msa1308020:three_prime_UTR	30.0%
	AAACGAGTAACCTCATTATT+GGG	+	chr8_4:4163952-4163971	Msa1308020:three_prime_UTR	30.0%
!!	AACACACATACAAGAGCATT+AGG	+	chr8_4:4162365-4162384	Msa1308020:five_prime_UTR	35.0%
!!	ATACAAGAGCATTAGGAAGA+TGG	+	chr8_4:4162372-4162391	Msa1308020:five_prime_UTR	35.0%
	CAAGATGTTGGGCAAATAAT+GGG	-	chr8_4:4162622-4162641	None:intergenic	35.0%
	ACAAGATGTTGGGCAAATAA+TGG	-	chr8_4:4162623-4162642	None:intergenic	35.0%
	AACGTTGCTTACAAGATGTT+GGG	-	chr8_4:4162633-4162652	None:intergenic	35.0%
	GATGTTTGTTCAAAAGGAAG+AGG	-	chr8_4:4162676-4162695	None:intergenic	35.0%
!!	TCATGGTTAAGTTTTTGGAG+TGG	+	chr8_4:4163055-4163074	Msa1308020:intron	35.0%
	AAAAACTACAGAAACGTGTG+AGG	+	chr8_4:4163128-4163147	Msa1308020:CDS	35.0%
	TCCTCAATTCCAAATACTCA+AGG	+	chr8_4:4163211-4163230	Msa1308020:CDS	35.0%
!	ACCTTGAGTATTTGGAATTG+AGG	-	chr8_4:4163215-4163234	None:intergenic	35.0%
	TATTAGGAAACAGATCGATG+AGG	-	chr8_4:4163308-4163327	None:intergenic	35.0%
	TTGAATTCTCTGCACTTGTT+TGG	-	chr8_4:4163438-4163457	None:intergenic	35.0%
!!	GTAATAGGTGGTCTTATACA+AGG	-	chr8_4:4163471-4163490	None:intergenic	35.0%
	GTATTATCATGAGGAGAAGT+GGG	-	chr8_4:4163533-4163552	None:intergenic	35.0%
	AGTATTATCATGAGGAGAAG+TGG	-	chr8_4:4163534-4163553	None:intergenic	35.0%
	GAGGAAGTAGTATTATCATG+AGG	-	chr8_4:4163542-4163561	None:intergenic	35.0%
	GTTAAAGCTAAGCAATATGG+AGG	-	chr8_4:4163561-4163580	None:intergenic	35.0%
	GATGTCGACGATGATAAAAA+GGG	-	chr8_4:4163617-4163636	None:intergenic	35.0%
	TGATGTCGACGATGATAAAA+AGG	-	chr8_4:4163618-4163637	None:intergenic	35.0%
	ACTTTCAGTGGAATATTGCA+AGG	+	chr8_4:4163661-4163680	Msa1308020:CDS	35.0%
	ACTTGAATCTGATGATCACA+GGG	+	chr8_4:4163787-4163806	Msa1308020:CDS	35.0%
!	TGAGGTAATGGTAACAGAAA+AGG	+	chr8_4:4164015-4164034	Msa1308020:three_prime_UTR	35.0%
!!	AACACACATACAAGAGCATT+AGG	+	chr8_4:4162365-4162384	Msa1308020:five_prime_UTR	35.0%
!!	ATACAAGAGCATTAGGAAGA+TGG	+	chr8_4:4162372-4162391	Msa1308020:five_prime_UTR	35.0%
	CAAGATGTTGGGCAAATAAT+GGG	-	chr8_4:4162622-4162641	None:intergenic	35.0%
	ACAAGATGTTGGGCAAATAA+TGG	-	chr8_4:4162623-4162642	None:intergenic	35.0%
	AACGTTGCTTACAAGATGTT+GGG	-	chr8_4:4162633-4162652	None:intergenic	35.0%
	GATGTTTGTTCAAAAGGAAG+AGG	-	chr8_4:4162676-4162695	None:intergenic	35.0%
!!	TCATGGTTAAGTTTTTGGAG+TGG	+	chr8_4:4163055-4163074	Msa1308020:intron	35.0%
	AAAAACTACAGAAACGTGTG+AGG	+	chr8_4:4163128-4163147	Msa1308020:CDS	35.0%
	TCCTCAATTCCAAATACTCA+AGG	+	chr8_4:4163211-4163230	Msa1308020:CDS	35.0%
!	ACCTTGAGTATTTGGAATTG+AGG	-	chr8_4:4163215-4163234	None:intergenic	35.0%
	TATTAGGAAACAGATCGATG+AGG	-	chr8_4:4163308-4163327	None:intergenic	35.0%
	TTGAATTCTCTGCACTTGTT+TGG	-	chr8_4:4163438-4163457	None:intergenic	35.0%
!!	GTAATAGGTGGTCTTATACA+AGG	-	chr8_4:4163471-4163490	None:intergenic	35.0%
	GTATTATCATGAGGAGAAGT+GGG	-	chr8_4:4163533-4163552	None:intergenic	35.0%
	AGTATTATCATGAGGAGAAG+TGG	-	chr8_4:4163534-4163553	None:intergenic	35.0%
	GAGGAAGTAGTATTATCATG+AGG	-	chr8_4:4163542-4163561	None:intergenic	35.0%
	GTTAAAGCTAAGCAATATGG+AGG	-	chr8_4:4163561-4163580	None:intergenic	35.0%
	GATGTCGACGATGATAAAAA+GGG	-	chr8_4:4163617-4163636	None:intergenic	35.0%
	TGATGTCGACGATGATAAAA+AGG	-	chr8_4:4163618-4163637	None:intergenic	35.0%
	ACTTTCAGTGGAATATTGCA+AGG	+	chr8_4:4163661-4163680	Msa1308020:CDS	35.0%
	ACTTGAATCTGATGATCACA+GGG	+	chr8_4:4163787-4163806	Msa1308020:CDS	35.0%
!!	AAGAGCATTAGGAAGATGGA+AGG	+	chr8_4:4162376-4162395	Msa1308020:five_prime_UTR	40.0%
	CATTAGGAAGATGGAAGGAA+GGG	+	chr8_4:4162381-4162400	Msa1308020:five_prime_UTR	40.0%
	TGATAGAGATGAGGCAATGA+AGG	+	chr8_4:4162514-4162533	Msa1308020:CDS	40.0%
	CAACGTTGCTTACAAGATGT+TGG	-	chr8_4:4162634-4162653	None:intergenic	40.0%
	CTAGAGGATCTCACAAAAGA+AGG	+	chr8_4:4162780-4162799	Msa1308020:CDS	40.0%
!	AGTCATGTTGTTTGCTAGCT+AGG	+	chr8_4:4163010-4163029	Msa1308020:intron	40.0%
	TCAGAAAGACCAACAGATGA+TGG	+	chr8_4:4163156-4163175	Msa1308020:CDS	40.0%
	CAGAAAGACCAACAGATGAT+GGG	+	chr8_4:4163157-4163176	Msa1308020:CDS	40.0%
	ATATATGCAGGGACTACTAC+AGG	+	chr8_4:4163378-4163397	Msa1308020:intron	40.0%
	TGTCTTTGTTACAAGTGTGG+TGG	-	chr8_4:4163496-4163515	None:intergenic	40.0%
	GCAATATTCCACTGAAAGTG+AGG	-	chr8_4:4163660-4163679	None:intergenic	40.0%
	CACTTGAATCTGATGATCAC+AGG	+	chr8_4:4163786-4163805	Msa1308020:CDS	40.0%
!	GATTTCCTTCACCCTTTTCA+TGG	+	chr8_4:4163863-4163882	Msa1308020:CDS	40.0%
	CATGCATTGTCCCAATAATG+AGG	-	chr8_4:4163965-4163984	None:intergenic	40.0%
	GACAATGCATGTGATGACTT+TGG	+	chr8_4:4163974-4163993	Msa1308020:three_prime_UTR	40.0%
!!	AAGAGCATTAGGAAGATGGA+AGG	+	chr8_4:4162376-4162395	Msa1308020:five_prime_UTR	40.0%
	CATTAGGAAGATGGAAGGAA+GGG	+	chr8_4:4162381-4162400	Msa1308020:five_prime_UTR	40.0%
	TGATAGAGATGAGGCAATGA+AGG	+	chr8_4:4162514-4162533	Msa1308020:CDS	40.0%
	CAACGTTGCTTACAAGATGT+TGG	-	chr8_4:4162634-4162653	None:intergenic	40.0%
	CTAGAGGATCTCACAAAAGA+AGG	+	chr8_4:4162780-4162799	Msa1308020:CDS	40.0%
!	AGTCATGTTGTTTGCTAGCT+AGG	+	chr8_4:4163010-4163029	Msa1308020:intron	40.0%
	TCAGAAAGACCAACAGATGA+TGG	+	chr8_4:4163156-4163175	Msa1308020:CDS	40.0%
	CAGAAAGACCAACAGATGAT+GGG	+	chr8_4:4163157-4163176	Msa1308020:CDS	40.0%
	ATATATGCAGGGACTACTAC+AGG	+	chr8_4:4163378-4163397	Msa1308020:intron	40.0%
	TGTCTTTGTTACAAGTGTGG+TGG	-	chr8_4:4163496-4163515	None:intergenic	40.0%
	GCAATATTCCACTGAAAGTG+AGG	-	chr8_4:4163660-4163679	None:intergenic	40.0%
	CACTTGAATCTGATGATCAC+AGG	+	chr8_4:4163786-4163805	Msa1308020:CDS	40.0%
!	GATTTCCTTCACCCTTTTCA+TGG	+	chr8_4:4163863-4163882	Msa1308020:CDS	40.0%
	CATGCATTGTCCCAATAATG+AGG	-	chr8_4:4163965-4163984	None:intergenic	40.0%
	GACAATGCATGTGATGACTT+TGG	+	chr8_4:4163974-4163993	Msa1308020:three_prime_UTR	40.0%
	GCATTAGGAAGATGGAAGGA+AGG	+	chr8_4:4162380-4162399	Msa1308020:five_prime_UTR	45.0%
	CAATGAAGGAGCTTGTGAGA+GGG	+	chr8_4:4162528-4162547	Msa1308020:CDS	45.0%
	CATTCATGCCCATCATCTGT+TGG	-	chr8_4:4163168-4163187	None:intergenic	45.0%
!!	CAACAGATGATGGGCATGAA+TGG	+	chr8_4:4163166-4163185	Msa1308020:CDS	45.0%
	GGGCATGAATGGAGAAAGTA+TGG	+	chr8_4:4163177-4163196	Msa1308020:CDS	45.0%
	TAGGAAACAGATCGATGAGG+AGG	-	chr8_4:4163305-4163324	None:intergenic	45.0%
	TCTGCACTTGTTTGGTTGCT+TGG	-	chr8_4:4163430-4163449	None:intergenic	45.0%
!!	TAGGTGGTCTTATACAAGGG+AGG	-	chr8_4:4163467-4163486	None:intergenic	45.0%
	CAATCTTGTTCGGTTGGAGT+TGG	-	chr8_4:4163593-4163612	None:intergenic	45.0%
	AAGGGACAATCTTGTTCGGT+TGG	-	chr8_4:4163599-4163618	None:intergenic	45.0%
	AGCAGGTATTCATCCAAGGA+AGG	-	chr8_4:4163695-4163714	None:intergenic	45.0%
	AGAGAGCAGGTATTCATCCA+AGG	-	chr8_4:4163699-4163718	None:intergenic	45.0%
!	AAATTGGTTCAGGAGAGAGC+AGG	-	chr8_4:4163712-4163731	None:intergenic	45.0%
	GAAAGCCATGAAAAGGGTGA+AGG	-	chr8_4:4163871-4163890	None:intergenic	45.0%
	GCATTAGGAAGATGGAAGGA+AGG	+	chr8_4:4162380-4162399	Msa1308020:five_prime_UTR	45.0%
	CAATGAAGGAGCTTGTGAGA+GGG	+	chr8_4:4162528-4162547	Msa1308020:CDS	45.0%
	CATTCATGCCCATCATCTGT+TGG	-	chr8_4:4163168-4163187	None:intergenic	45.0%
!!	CAACAGATGATGGGCATGAA+TGG	+	chr8_4:4163166-4163185	Msa1308020:CDS	45.0%
	GGGCATGAATGGAGAAAGTA+TGG	+	chr8_4:4163177-4163196	Msa1308020:CDS	45.0%
	TAGGAAACAGATCGATGAGG+AGG	-	chr8_4:4163305-4163324	None:intergenic	45.0%
	TCTGCACTTGTTTGGTTGCT+TGG	-	chr8_4:4163430-4163449	None:intergenic	45.0%
!!	TAGGTGGTCTTATACAAGGG+AGG	-	chr8_4:4163467-4163486	None:intergenic	45.0%
	CAATCTTGTTCGGTTGGAGT+TGG	-	chr8_4:4163593-4163612	None:intergenic	45.0%
	AAGGGACAATCTTGTTCGGT+TGG	-	chr8_4:4163599-4163618	None:intergenic	45.0%
	AGCAGGTATTCATCCAAGGA+AGG	-	chr8_4:4163695-4163714	None:intergenic	45.0%
	AGAGAGCAGGTATTCATCCA+AGG	-	chr8_4:4163699-4163718	None:intergenic	45.0%
!	AAATTGGTTCAGGAGAGAGC+AGG	-	chr8_4:4163712-4163731	None:intergenic	45.0%
	GAAAGCCATGAAAAGGGTGA+AGG	-	chr8_4:4163871-4163890	None:intergenic	45.0%
	GCAATGAAGGAGCTTGTGAG+AGG	+	chr8_4:4162527-4162546	Msa1308020:CDS	50.0%
!	TACAAGACTAGTTCCACCCG+TGG	+	chr8_4:4162755-4162774	Msa1308020:CDS	50.0%
	TGTGAGATCCTCTAGCACCA+CGG	-	chr8_4:4162775-4162794	None:intergenic	50.0%
	ACAAGTGTGGTGGCCGTAAT+AGG	-	chr8_4:4163486-4163505	None:intergenic	50.0%
	CAAGGAGAACTCACCTTCCT+TGG	+	chr8_4:4163679-4163698	Msa1308020:CDS	50.0%
	GCAATGAAGGAGCTTGTGAG+AGG	+	chr8_4:4162527-4162546	Msa1308020:CDS	50.0%
!	TACAAGACTAGTTCCACCCG+TGG	+	chr8_4:4162755-4162774	Msa1308020:CDS	50.0%
	TGTGAGATCCTCTAGCACCA+CGG	-	chr8_4:4162775-4162794	None:intergenic	50.0%
	ACAAGTGTGGTGGCCGTAAT+AGG	-	chr8_4:4163486-4163505	None:intergenic	50.0%
	CAAGGAGAACTCACCTTCCT+TGG	+	chr8_4:4163679-4163698	Msa1308020:CDS	50.0%
	GTGAGATCCTCTAGCACCAC+GGG	-	chr8_4:4162774-4162793	None:intergenic	55.0%
	AGTGTGGTGGCCGTAATAGG+TGG	-	chr8_4:4163483-4163502	None:intergenic	55.0%
	GTGAGATCCTCTAGCACCAC+GGG	-	chr8_4:4162774-4162793	None:intergenic	55.0%
	AGTGTGGTGGCCGTAATAGG+TGG	-	chr8_4:4163483-4163502	None:intergenic	55.0%
!	AGTTCCACCCGTGGTGCTAG+AGG	+	chr8_4:4162764-4162783	Msa1308020:CDS	60.0%
	AGATCCTCTAGCACCACGGG+TGG	-	chr8_4:4162771-4162790	None:intergenic	60.0%
!	AGTTCCACCCGTGGTGCTAG+AGG	+	chr8_4:4162764-4162783	Msa1308020:CDS	60.0%
	AGATCCTCTAGCACCACGGG+TGG	-	chr8_4:4162771-4162790	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	4162347	4164036	4162347	ID=Msa1308020;Name=Msa1308020
chr8_4	mRNA	4162347	4164036	4162347	ID=Msa1308020-mRNA-1;Parent=Msa1308020;Name=Msa1308020-mRNA-1;_AED=0.24;_eAED=0.24;_QI=162\|1\|1\|1\|1\|1\|3\|138\|302
chr8_4	exon	4162347	4162801	4162347	ID=Msa1308020-mRNA-1:exon:1197;Parent=Msa1308020-mRNA-1
chr8_4	exon	4163128	4163232	4163128	ID=Msa1308020-mRNA-1:exon:1198;Parent=Msa1308020-mRNA-1
chr8_4	exon	4163388	4164036	4163388	ID=Msa1308020-mRNA-1:exon:1199;Parent=Msa1308020-mRNA-1
chr8_4	five_prime_UTR	4162347	4162508	4162347	ID=Msa1308020-mRNA-1:five_prime_utr;Parent=Msa1308020-mRNA-1
chr8_4	CDS	4162509	4162801	4162509	ID=Msa1308020-mRNA-1:cds;Parent=Msa1308020-mRNA-1
chr8_4	CDS	4163128	4163232	4163128	ID=Msa1308020-mRNA-1:cds;Parent=Msa1308020-mRNA-1
chr8_4	CDS	4163388	4163898	4163388	ID=Msa1308020-mRNA-1:cds;Parent=Msa1308020-mRNA-1
chr8_4	three_prime_UTR	4163899	4164036	4163899	ID=Msa1308020-mRNA-1:three_prime_utr;Parent=Msa1308020-mRNA-1

Gene Sequence

>Msa1308020

ATGGATGATAGAGATGAGGCAATGAAGGAGCTTGTGAGAGGGCGTGAGTTAGCTAAGCAGCTTGAACAACATCTCAGTAACAACAACAACATTAATGATGAATTAGCAAGCCCATTATTTGCCCAACATCTTGTAAGCAACGTTGTCAACTCATTTAGTAACACCCTCTTCCTTTTGAACAAACATCATCAAAGGGAACAGAACTTTTCTTTAACTATTAAATCTGAAGAGGAATCTCAAGATAGTTACAAGACTAGTTCCACCCGTGGTGCTAGAGGATCTCACAAAAGAAGAAAAACTACAGAAACGTGTGAGGAAGTGTCAGAAAGACCAACAGATGATGGGCATGAATGGAGAAAGTATGGTCAAAAAACAATCCTCAATTCCAAATACTCAAGGGACTACTACAGGTGTACTCACAAGTTTAATCAAAGATGCCAAGCAACCAAACAAGTGCAGAGAATTCAAGAGAAGCCTCCCTTGTATAAGACCACCTATTACGGCCACCACACTTGTAACAAAGACAATATCATATTTGAACCCACTTCTCCTCATGATAATACTACTTCCTCCATATTGCTTAGCTTTAACAACACATTTCCAACTCCAACCGAACAAGATTGTCCCTTTTTATCATCGTCGACATCATCATCTTCGTCTTTTAATTCCTCACTTTCAGTGGAATATTGCAAGGAGAACTCACCTTCCTTGGATGAATACCTGCTCTCTCCTGAACCAATTTTAGATAAATCTATTTCTACAAGGCATGTTCGTAGTACTCTATCATCATCAACTACACTTGAATCTGATGATCACAGGGATATGATGATAATATTGTATGGTCTCTTGTATGATTCTGTTGAACTTGATACTGATTTCCTTCACCCTTTTCATGGCTTTCAATTATAG

Protein sequence

>Msa1308020

MDDRDEAMKELVRGRELAKQLEQHLSNNNNINDELASPLFAQHLVSNVVNSFSNTLFLLNKHHQREQNFSLTIKSEEESQDSYKTSSTRGARGSHKRRKTTETCEEVSERPTDDGHEWRKYGQKTILNSKYSRDYYRCTHKFNQRCQATKQVQRIQEKPPLYKTTYYGHHTCNKDNIIFEPTSPHDNTTSSILLSFNNTFPTPTEQDCPFLSSSTSSSSSFNSSLSVEYCKENSPSLDEYLLSPEPILDKSISTRHVRSTLSSSTTLESDDHRDMMIILYGLLYDSVELDTDFLHPFHGFQL*