AlfalfaGEDB | Msa1309000

M. sativa cultivar zhongmu-4 / Msa1309000

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309000	KAE9607265.1	60.274	73	29	0	12	84	59	131	7.76e-19	95.5
Msa1309000	KAE9607265.1	72.662	139	38	0	78	216	289	427	2.72e-65	218

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309000	sp\|Q9SZL8\|FRS5_ARATH	36.111	144	88	2	73	215	331	471	5.48e-24	102

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309000	A0A6A4PZP9	60.274	73	29	0	12	84	59	131	3.71e-19	95.5
Msa1309000	A0A6A4PZP9	72.662	139	38	0	78	216	289	427	1.30e-65	218

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TFs/TRs:

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Gene ID	Type	Classification
Msa1309000	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309000	MtrunA17_Chr1g0164591	52.500	120	57	0	78	197	173	292	1.13e-39	138
Msa1309000	MtrunA17_Chr3g0082121	52.137	117	56	0	78	194	293	409	1.90e-37	137
Msa1309000	MtrunA17_Chr6g0458391	44.538	119	66	0	78	196	465	583	5.59e-35	130
Msa1309000	MtrunA17_Chr6g0458391	50.704	71	32	2	12	80	236	305	5.45e-14	70.5
Msa1309000	MtrunA17_Chr1g0203721	45.082	122	67	0	76	197	321	442	8.59e-33	124
Msa1309000	MtrunA17_Chr1g0203721	49.296	71	33	2	12	80	74	143	6.41e-15	73.2
Msa1309000	MtrunA17_Chr1g0149131	50.505	99	49	0	99	197	1	99	3.90e-32	113
Msa1309000	MtrunA17_Chr2g0324011	43.333	120	68	0	78	197	267	386	1.35e-31	120
Msa1309000	MtrunA17_Chr1g0203051	40.833	120	66	1	78	197	337	451	3.03e-27	108
Msa1309000	MtrunA17_Chr5g0448761	37.226	137	82	2	73	208	322	455	2.04e-26	106

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1309000	AT4G38180.1	36.111	144	88	2	73	215	331	471	5.57e-25	102
Msa1309000	AT3G06250.2	36.667	120	73	2	78	197	409	525	1.55e-23	98.6
Msa1309000	AT3G06250.1	36.667	120	73	2	78	197	409	525	1.55e-23	98.6
Msa1309000	AT3G06250.3	36.667	120	73	2	78	197	409	525	1.55e-23	98.6
Msa1309000	AT5G18960.2	33.333	132	82	2	67	198	425	550	1.15e-22	95.9
Msa1309000	AT5G18960.1	33.333	132	82	2	67	198	425	550	1.15e-22	95.9
Msa1309000	AT3G22170.2	32.117	137	89	2	73	208	316	449	6.71e-22	93.6
Msa1309000	AT3G22170.1	32.117	137	89	2	73	208	316	449	6.71e-22	93.6
Msa1309000	AT1G80010.1	33.333	144	91	3	70	212	350	489	8.41e-20	87.8
Msa1309000	AT2G27110.3	33.571	140	89	2	73	211	133	269	3.40e-19	85.9

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Find 22 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAATTAGATCGCTGAA+AGG	0.605255	8_4:-5398711	None:intergenic
AAACAAAAGTAAATTATACA+AGG	0.614847	8_4:+5398156	Msa1309000:CDS
AACAGATCAAGATCCTGCAA+TGG	0.602312	8_4:+5398598	Msa1309000:CDS
AAGAGAATAAAAGATGAAGA+AGG	0.531444	8_4:+5398223	Msa1309000:CDS
AATATTTACGCAGGCTTTGT+TGG	0.382142	8_4:+5398109	Msa1309000:CDS
AATCTTGCATCCATTTATTT+TGG	0.162980	8_4:-5398826	None:intergenic
AATGGCAAAGGCAATTCCAG+AGG	0.688167	8_4:+5398616	Msa1309000:CDS
ACTCGCAAAGATATCAGAAC+TGG	0.524113	8_4:+5398253	Msa1309000:CDS
AGAACTGGTTGTTCAGCATA+TGG	0.459557	8_4:+5398268	Msa1309000:CDS
AGACAGCTGAATCATTTGAG+TGG	0.678572	8_4:+5398522	Msa1309000:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATCGAATGGAAAATATG+TGG	+	chr8_4:5398301-5398320	Msa1309000:intron	30.0%
AGAATCGAATGGAAAATATG+TGG	+	chr8_4:5398301-5398320	Msa1309000:intron	30.0%
CATAAACGATGATATGACTC+GGG	-	chr8_4:5398647-5398666	None:intergenic	35.0%
GCATAAACGATGATATGACT+CGG	-	chr8_4:5398648-5398667	None:intergenic	35.0%
CATATCATCGTTTATGCTTG+TGG	+	chr8_4:5398651-5398670	Msa1309000:CDS	35.0%
GAGTTGCAAAGTTGCATTTA+TGG	+	chr8_4:5398740-5398759	Msa1309000:CDS	35.0%
CATAAACGATGATATGACTC+GGG	-	chr8_4:5398647-5398666	None:intergenic	35.0%
GCATAAACGATGATATGACT+CGG	-	chr8_4:5398648-5398667	None:intergenic	35.0%
CATATCATCGTTTATGCTTG+TGG	+	chr8_4:5398651-5398670	Msa1309000:CDS	35.0%
GAGTTGCAAAGTTGCATTTA+TGG	+	chr8_4:5398740-5398759	Msa1309000:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	5398058	5398925	5398058	ID=Msa1309000;Name=Msa1309000
chr8_4	mRNA	5398058	5398925	5398058	ID=Msa1309000-mRNA-1;Parent=Msa1309000;Name=Msa1309000-mRNA-1;_AED=0.35;_eAED=0.37;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|216
chr8_4	exon	5398058	5398289	5398058	ID=Msa1309000-mRNA-1:exon:1739;Parent=Msa1309000-mRNA-1
chr8_4	exon	5398507	5398925	5398507	ID=Msa1309000-mRNA-1:exon:1740;Parent=Msa1309000-mRNA-1
chr8_4	CDS	5398058	5398289	5398058	ID=Msa1309000-mRNA-1:cds;Parent=Msa1309000-mRNA-1
chr8_4	CDS	5398507	5398925	5398507	ID=Msa1309000-mRNA-1:cds;Parent=Msa1309000-mRNA-1

Gene Sequence

>Msa1309000

ATGTTGGTATGGAATTTGATACTGAAGATCATATATGCATATAGGTTTTATAATATTTACGCAGGCTTTGTTGGTTTTAGCATTAGAAAAGATTGGAGAAACAAAAGTAAATTATACAAGGAGATTGTGATATCTCGAAAATTTTCTTGTTTTGAAGAAGGCTTCAAGAGAATAAAAGATGAAGAAGGAAAAGTGACTCGCAAAGATATCAGAACTGGTTGTTCAGCATATGCATTATTATATGATGAGACAGCTGAATCATTTGAGTGGTTGTTCAAGACTTTTCTTAAAGCTATGTGTGGTAAAAAGCCGATGACAATTTTAACAGATCAAGATCCTGCAATGGCAAAGGCAATTCCAGAGGTTTTTCCCGAGTCATATCATCGTTTATGCTTGTGGCATTTATTTCAAAATGCACTAAAGAATGTTAATCATGCCTTTCAGCGATCTAATTATTTTGCTAGCGAGTTGCAAAGTTGCATTTATGGTTTCGAGTATGAGGATGATTTTCTGAATGCTTGGGAGTCATTACTTAACAAGCATAATCTTCGCCAAAATAAATGGATGCAAGATTTCTTTAAAAAAAGGAAAAATGGGCTTCGGTATATGGGCGACACACTTTTTCAGCCAGTGCTACAACAACACAATTAA

Protein sequence

>Msa1309000

MLVWNLILKIIYAYRFYNIYAGFVGFSIRKDWRNKSKLYKEIVISRKFSCFEEGFKRIKDEEGKVTRKDIRTGCSAYALLYDETAESFEWLFKTFLKAMCGKKPMTILTDQDPAMAKAIPEVFPESYHRLCLWHLFQNALKNVNHAFQRSNYFASELQSCIYGFEYEDDFLNAWESLLNKHNLRQNKWMQDFFKKRKNGLRYMGDTLFQPVLQQHN*