Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312620 | XP_003627072.3 | 83.028 | 872 | 143 | 2 | 1 | 868 | 6 | 876 | 0.0 | 1510 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312620 | sp|C0LGD6|Y1570_ARATH | 48.541 | 857 | 415 | 17 | 5 | 844 | 4 | 851 | 0.0 | 771 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312620 | A0A396GKI3 | 83.028 | 872 | 143 | 2 | 1 | 868 | 1 | 871 | 0.0 | 1508 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312620 | MtrunA17_Chr8g0340931 | 83.028 | 872 | 143 | 2 | 1 | 868 | 1 | 871 | 0.0 | 1508 |
| Msa1312620 | MtrunA17_Chr8g0340911 | 77.460 | 874 | 183 | 9 | 1 | 868 | 6 | 871 | 0.0 | 1376 |
| Msa1312620 | MtrunA17_Chr8g0340881 | 76.914 | 875 | 191 | 8 | 1 | 868 | 6 | 876 | 0.0 | 1375 |
| Msa1312620 | MtrunA17_Chr8g0340891 | 76.640 | 869 | 196 | 6 | 1 | 868 | 6 | 868 | 0.0 | 1356 |
| Msa1312620 | MtrunA17_Chr8g0340871 | 76.818 | 880 | 188 | 10 | 1 | 868 | 6 | 881 | 0.0 | 1342 |
| Msa1312620 | MtrunA17_Chr8g0341151 | 75.457 | 876 | 195 | 8 | 1 | 868 | 2 | 865 | 0.0 | 1329 |
| Msa1312620 | MtrunA17_Chr8g0341011 | 74.592 | 858 | 203 | 9 | 20 | 868 | 24 | 875 | 0.0 | 1307 |
| Msa1312620 | MtrunA17_Chr8g0347661 | 73.349 | 878 | 218 | 10 | 1 | 868 | 6 | 877 | 0.0 | 1266 |
| Msa1312620 | MtrunA17_Chr8g0358831 | 65.182 | 853 | 229 | 11 | 1 | 840 | 14 | 811 | 0.0 | 1076 |
| Msa1312620 | MtrunA17_Chr8g0341101 | 55.231 | 889 | 339 | 16 | 22 | 868 | 51 | 922 | 0.0 | 894 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312620 | AT1G05700.1 | 48.541 | 857 | 415 | 17 | 5 | 844 | 4 | 851 | 0.0 | 771 |
| Msa1312620 | AT4G29990.1 | 47.262 | 840 | 420 | 11 | 15 | 836 | 12 | 846 | 0.0 | 768 |
| Msa1312620 | AT1G05700.2 | 47.248 | 872 | 407 | 16 | 18 | 844 | 19 | 882 | 0.0 | 758 |
| Msa1312620 | AT2G19210.1 | 45.276 | 868 | 442 | 17 | 8 | 852 | 9 | 866 | 0.0 | 751 |
| Msa1312620 | AT2G19230.2 | 46.102 | 885 | 435 | 18 | 1 | 860 | 1 | 868 | 0.0 | 748 |
| Msa1312620 | AT2G19230.1 | 46.050 | 886 | 435 | 18 | 1 | 860 | 1 | 869 | 0.0 | 746 |
| Msa1312620 | AT1G51880.1 | 44.912 | 855 | 442 | 18 | 5 | 843 | 4 | 845 | 0.0 | 722 |
| Msa1312620 | AT1G51880.2 | 44.912 | 855 | 442 | 18 | 5 | 843 | 22 | 863 | 0.0 | 722 |
| Msa1312620 | AT1G51790.3 | 45.309 | 874 | 437 | 18 | 12 | 858 | 11 | 870 | 0.0 | 722 |
| Msa1312620 | AT1G51790.1 | 45.309 | 874 | 437 | 18 | 12 | 858 | 11 | 870 | 0.0 | 722 |
Find 174 sgRNAs with CRISPR-Local
Find 353 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTACTATTGGAGAAGG+AGG | 0.594587 | 8_4:+9685824 | Msa1312620:CDS |
| AAAATCCAAAAGAAATCAAA+AGG | 0.477655 | 8_4:-9685362 | None:intergenic |
| AAAATGATATCTACAGCTTT+GGG | 0.378641 | 8_4:+9687998 | Msa1312620:CDS |
| AAAATTCAAATAACTTAAGT+TGG | 0.418230 | 8_4:+9686632 | Msa1312620:CDS |
| AAACAGGTTCATTATCACTG+AGG | 0.742559 | 8_4:+9685040 | Msa1312620:CDS |
| AAACATCAAGAATGCTTATG+GGG | 0.623307 | 8_4:+9684349 | Msa1312620:CDS |
| AAACCATTCACATACTTCAA+TGG | 0.393496 | 8_4:+9688076 | Msa1312620:CDS |
| AAACTCAAGTCGCTGTTAAG+AGG | 0.413756 | 8_4:+9685882 | Msa1312620:CDS |
| AAAGGGGCACTAGCATTTAC+TGG | 0.388965 | 8_4:-9683619 | None:intergenic |
| AAAGTGTTGGAGATTTCCAT+TGG | 0.522599 | 8_4:-9686177 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TACACCTGTATCTATGAATT+TGG | - | chr8_4:9682935-9682954 | None:intergenic | 30.0% | |
| AAATGAAGATCAAATTCTGG+TGG | - | chr8_4:9683123-9683142 | None:intergenic | 30.0% | |
| CCAAAATGAAGATCAAATTC+TGG | - | chr8_4:9683126-9683145 | None:intergenic | 30.0% | |
| GTAAATTATCTCCTTGATTG+TGG | - | chr8_4:9683196-9683215 | None:intergenic | 30.0% | |
| TCATTTCAGTTGTAGAATTG+AGG | + | chr8_4:9683274-9683293 | Msa1312620:CDS | 30.0% | |
| GGTGATAATACTGATGATTT+AGG | - | chr8_4:9683333-9683352 | None:intergenic | 30.0% | |
| TTCGTCTCATTCAATTGAAA+AGG | - | chr8_4:9683520-9683539 | None:intergenic | 30.0% | |
| TGAATTCTGTGTCTTAGAAA+GGG | - | chr8_4:9683845-9683864 | None:intergenic | 30.0% | |
| TTGAATTCTGTGTCTTAGAA+AGG | - | chr8_4:9683846-9683865 | None:intergenic | 30.0% | |
| GAGGATACTATATTTAAGGA+CGG | - | chr8_4:9683989-9684008 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 9682682 | 9688631 | 9682682 | ID=Msa1312620;Name=Msa1312620 |
| chr8_4 | mRNA | 9682682 | 9688631 | 9682682 | ID=Msa1312620-mRNA-1;Parent=Msa1312620;Name=Msa1312620-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0.83|0.76|1|1|1|13|257|868 |
| chr8_4 | exon | 9682682 | 9682766 | 9682682 | ID=Msa1312620-mRNA-1:exon:3641;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9682851 | 9683397 | 9682851 | ID=Msa1312620-mRNA-1:exon:3642;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9683483 | 9683943 | 9683483 | ID=Msa1312620-mRNA-1:exon:3643;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9684336 | 9684477 | 9684336 | ID=Msa1312620-mRNA-1:exon:3644;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9684563 | 9684634 | 9684563 | ID=Msa1312620-mRNA-1:exon:3645;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9684817 | 9684888 | 9684817 | ID=Msa1312620-mRNA-1:exon:3646;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9684981 | 9685061 | 9684981 | ID=Msa1312620-mRNA-1:exon:3647;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9685266 | 9685405 | 9685266 | ID=Msa1312620-mRNA-1:exon:3648;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9685726 | 9685949 | 9685726 | ID=Msa1312620-mRNA-1:exon:3649;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9686080 | 9686203 | 9686080 | ID=Msa1312620-mRNA-1:exon:3650;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9686629 | 9686691 | 9686629 | ID=Msa1312620-mRNA-1:exon:3651;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9686764 | 9686953 | 9686764 | ID=Msa1312620-mRNA-1:exon:3652;Parent=Msa1312620-mRNA-1 |
| chr8_4 | exon | 9687969 | 9688631 | 9687969 | ID=Msa1312620-mRNA-1:exon:3653;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9682682 | 9682766 | 9682682 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9682851 | 9683397 | 9682851 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9683483 | 9683943 | 9683483 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9684336 | 9684477 | 9684336 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9684563 | 9684634 | 9684563 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9684817 | 9684888 | 9684817 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9684981 | 9685061 | 9684981 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9685266 | 9685405 | 9685266 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9685726 | 9685949 | 9685726 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9686080 | 9686203 | 9686080 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9686629 | 9686691 | 9686629 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9686764 | 9686953 | 9686764 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | CDS | 9687969 | 9688374 | 9687969 | ID=Msa1312620-mRNA-1:cds;Parent=Msa1312620-mRNA-1 |
| chr8_4 | three_prime_UTR | 9688375 | 9688631 | 9688375 | ID=Msa1312620-mRNA-1:three_prime_utr;Parent=Msa1312620-mRNA-1 |
| Gene Sequence |
>Msa1312620 ATGGGAATGTTAATGCATTTTCTTTTGGTATTGTTTGGTGTTCTTGCTACTATTTTGGTTTTGATACAAGCTCAGGATCAATCAGGATTCATTAGCATAGATTGTGGCCTACCTGAACATTCAAGCTACTCTGATAGAATCACAGACCTAAGTTACATTTCAGACGCCAAATTCATAGATACAGGTGTAACTAAGAGGATACCTCCTACAAATATTATTATCAAACAGCAACTAGAATATGTGAGGAGCTTTCCTAGTGGAGTGAGAAATTGTTATAACATAAGTGTAACAAGTGGTACTAAATATTTAATCAGAGCTACTTTCTATTATGGTAACTATGATGGTCTAAATGAGCCACCAGAATTTGATCTTCATTTTGGAGCTAATGTATGGGATACAGTGAAATTGTCCTCCAATGCATCACGCGCCACAATCAAGGAGATAATTTACACTCCATTATTAGATTACATACAACCATGTCTTGTTAGCACAGGCAAAGGGACTCCATTCATTTCAGTTGTAGAATTGAGGACTTTGAACAATGAAACTTATGTCACAAACTCACCTAAATCATCAGTATTATCACCCCTCAGACGATCTGATGTAGGTTCCATCGCAGACAAACAATACAGGTACAAAGATGATGTTTACGATCGCATCTGGTTACCTTTTCAATTGAATGAGACGAAACGATTTAGCACCAATAAGGATCTATTAATTCAGAGTAGTTATAAACTACCAGCAATTGTGATGAGCACTGCTGTTACACCAGTAAATGCTAGTGCCCCTTTACAATTTCATTGGAATTCAGATAATATAAATGATCAATACTATCTTTACATGCACTTTTGTGAGGTTGAAGAGATGGCAGCAAATGATACTAGAGCGTTCAATATCTCAGTGAATGACAAGTTTCAGTATGGACCTGTGACACCATATACTACCATATTTAATAGAAAACCTATGACTGGAGCCAAAAGGTATCAAGTTTCCCTTTCTAAGACACAGAATTCAACCCTTCCACCTATTATCAATGCCTTTGAGGTTTATAAACTAAAAGACTTCTCAAAATCAGAAACTCAACAAGATGATGTTGATACTATGACAAACATCAAGAATGCTTATGGGGTTGCGAGAAATTGGCAAGGAGATCCATGTGGTCCTGTGAAGTATATGTGGGAAGGCCTAAACTGCAGTTTTGATGGTGGAAATAACCCCCCAAGAATCACATCTTTGAATTTGTCTTCAAGTGGATTGACAGGGGAGATACCATCTTCCATATCAAAGCTCGCTATGTTGCAATACTTGGATTTATCAAACAATAGCTTGAACGGTCCTTTACCTGATTTTCTGATGCAGCTGAGGTCGCTAAAAACCTTAAATGTAGGGAAGAACAAACTTACAGGGTCAGTTCCAAGTGGACTCCATGAGAGATCAAAAACAGGTTCATTATCACTGAGTGTCGATGATAATCCTGATCTTTGTATGATGGAATCCTGCAAAAAGAAGAACATTCTTGTACCACTTGTTGCATCGTTTTCAGCATTATTTGTAATCCTTTTGATTTCTTTTGGATTTTGGATATTCAGAAGACAAAAAGTTGTACTTACGTCTCCAAATTATAAGGAAAGGGGTTCAATGAAATCAAGACACCAAATATTCAGTTATTCTGAAATTCTCAACATTACTGATAACTTCAAAACTACTATTGGAGAAGGAGGATTTGGAAAAGTTTACTTCGGCATTCTACACGATCAAACTCAAGTCGCTGTTAAGAGGTTTTCACCATCATCAATGCAAGGTTATAAGGAATTTCAATCAGAGGCACAACTTCTAATGATTGTTCATCATAGAAATTTGGTTCCCCTCCTTGGATATTGTGATGAAGGTCAAATCAGAGCATTGATTTATGAATACATGGCCAATGGAAATCTCCAACACTTTTTAGTGGAAAATTCAAATAACTTAAGTTGGAATGAGAGGCTTAACATTGCAGTTGACACAGCACATGGATTAGATTATCTGCATAATGGATGTAAACCGCCTATTATGCATAGAGATTTGAAGCCTTCAAACATATTGCTAGATGAGAACTTACATGCAAAGATTGCCGATTTTGGTCTAAGTAGAGCTTTTGGTAATGATGATGATTCACATATATCTACACGCCCTGCTGGTACATTTGGTTACGCTGATCCAGTATATCAGAGAACAGGAAAAACAAATAAGAAAAATGATATCTACAGCTTTGGGATAATTCTATTTGAGTTGATCACTGGCCAAAAAGCACTAATAAAAGCAGCTGAAGAAACCATTCACATACTTCAATGGGTTATTCCCATAGTTGAAGGAGGAGATATTCAAAATGTTGTTGATTCAAGGTTGCGGGGTGAATTTAACATCAATTCAGCATGGAAAACAGTAGAGATTGCCATGTCATGCATTTCACAAACTGTATCTGACAGGCCAGACATAAGCCATATATTGGCAGGGTTAAAGGAATGTCTATGTTTGGAGATGGTTCAGAGAAATAATGGAAGCATGAGAGCTCCAGATGAATTCGAATCTGTAGCTACTGTATCAGAATCCACTATTTTAGCTAGATAA |
| Protein sequence |
>Msa1312620 MGMLMHFLLVLFGVLATILVLIQAQDQSGFISIDCGLPEHSSYSDRITDLSYISDAKFIDTGVTKRIPPTNIIIKQQLEYVRSFPSGVRNCYNISVTSGTKYLIRATFYYGNYDGLNEPPEFDLHFGANVWDTVKLSSNASRATIKEIIYTPLLDYIQPCLVSTGKGTPFISVVELRTLNNETYVTNSPKSSVLSPLRRSDVGSIADKQYRYKDDVYDRIWLPFQLNETKRFSTNKDLLIQSSYKLPAIVMSTAVTPVNASAPLQFHWNSDNINDQYYLYMHFCEVEEMAANDTRAFNISVNDKFQYGPVTPYTTIFNRKPMTGAKRYQVSLSKTQNSTLPPIINAFEVYKLKDFSKSETQQDDVDTMTNIKNAYGVARNWQGDPCGPVKYMWEGLNCSFDGGNNPPRITSLNLSSSGLTGEIPSSISKLAMLQYLDLSNNSLNGPLPDFLMQLRSLKTLNVGKNKLTGSVPSGLHERSKTGSLSLSVDDNPDLCMMESCKKKNILVPLVASFSALFVILLISFGFWIFRRQKVVLTSPNYKERGSMKSRHQIFSYSEILNITDNFKTTIGEGGFGKVYFGILHDQTQVAVKRFSPSSMQGYKEFQSEAQLLMIVHHRNLVPLLGYCDEGQIRALIYEYMANGNLQHFLVENSNNLSWNERLNIAVDTAHGLDYLHNGCKPPIMHRDLKPSNILLDENLHAKIADFGLSRAFGNDDDSHISTRPAGTFGYADPVYQRTGKTNKKNDIYSFGIILFELITGQKALIKAAEETIHILQWVIPIVEGGDIQNVVDSRLRGEFNINSAWKTVEIAMSCISQTVSDRPDISHILAGLKECLCLEMVQRNNGSMRAPDEFESVATVSESTILAR* |
