AlfalfaGEDB | Msa1312690

M. sativa cultivar zhongmu-4 / Msa1312690

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1312690	sp\|C0LGD9\|Y1756_ARATH	38.350	206	64	3	46	193	658	858	9.34e-35	133

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1312690	A0A2K3LUJ0	65.625	192	34	1	43	202	53	244	1.54e-78	244

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1312690	MtrunA17_Chr8g0341161	52.404	208	48	2	46	202	11	218	2.45e-67	205
Msa1312690	MtrunA17_Chr8g0340891	53.182	220	41	3	43	202	651	868	4.90e-63	209
Msa1312690	MtrunA17_Chr8g0340871	51.142	219	48	3	43	202	663	881	1.36e-54	186
Msa1312690	MtrunA17_Chr8g0341151	47.945	219	55	4	43	202	647	865	2.86e-53	182
Msa1312690	MtrunA17_Chr8g0341101	49.091	220	51	5	43	202	704	922	3.75e-52	179
Msa1312690	MtrunA17_Chr8g0340971	96.104	77	3	0	43	119	176	252	1.48e-50	164
Msa1312690	MtrunA17_Chr8g0340951	91.139	79	7	0	43	121	129	207	5.36e-50	161
Msa1312690	MtrunA17_Chr5g0423891	43.128	211	59	4	44	193	251	461	1.46e-47	162
Msa1312690	MtrunA17_Chr8g0340881	93.671	79	5	0	43	121	658	736	2.12e-47	166
Msa1312690	MtrunA17_Chr8g0341051	88.235	85	10	0	118	202	155	239	2.27e-47	155

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1312690	AT1G51805.2	36.530	219	77	4	45	202	643	860	1.81e-36	135
Msa1312690	AT1G51850.1	35.616	219	79	4	45	202	648	865	1.92e-36	135
Msa1312690	AT2G28970.1	35.185	216	78	4	48	202	572	786	2.06e-36	134
Msa1312690	AT1G51850.2	35.616	219	79	4	45	202	668	885	2.72e-36	134
Msa1312690	AT1G49100.1	35.321	218	77	4	47	202	673	888	2.97e-36	134
Msa1312690	AT4G29990.1	35.321	218	80	4	44	202	661	876	6.53e-36	133
Msa1312690	AT2G28970.2	35.185	216	78	4	48	202	537	751	7.08e-36	133
Msa1312690	AT1G07560.1	38.350	206	64	3	46	193	658	858	9.50e-36	133
Msa1312690	AT1G51805.1	36.530	219	77	4	45	202	667	884	1.70e-35	132
Msa1312690	AT2G28960.2	35.349	215	77	4	49	202	667	880	4.96e-35	130

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Find 31 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCAAATATCTTAAAC+TGG	0.399485	8_4:+9863094	Msa1312690:CDS
AAATCTCTATGCATAATAGG+TGG	0.620528	8_4:-9863414	None:intergenic
AAGTCAGCATCAATTCTGCA+TGG	0.626428	8_4:+9864083	Msa1312690:CDS
AATGCAAATAAATAGCACCA+AGG	0.694358	8_4:-9862576	None:intergenic
AGGAGATATTCAAAACATAA+TGG	0.449242	8_4:+9864042	Msa1312690:intron
ATAATGGATACGAGGTTGCA+GGG	0.612959	8_4:+9864058	Msa1312690:CDS
ATCAATATTTGGCTAATGTC+TGG	0.425119	8_4:-9864157	None:intergenic
ATTCTCATCTAGTAGTATGT+TGG	0.509240	8_4:-9863445	None:intergenic
ATTGCAGTTGATGCAGCACA+TGG	0.660665	8_4:+9863132	Msa1312690:CDS
ATTGCTAACAATGAAAATCC+TGG	0.530974	8_4:+9862644	Msa1312690:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACGATGGAGTTTAACATTA+AGG	-	chr8_4:9862549-9862568	None:intergenic	30.0%
AATGCAAATAAATAGCACCA+AGG	-	chr8_4:9862579-9862598	None:intergenic	30.0%
TTAGCAATTTCTCTTGAAAC+AGG	-	chr8_4:9862632-9862651	None:intergenic	30.0%
ATTGCTAACAATGAAAATCC+TGG	+	chr8_4:9862644-9862663	Msa1312690:CDS	30.0%
AACACCAAGTGCAAATATAT+GGG	-	chr8_4:9863356-9863375	None:intergenic	30.0%
AAATCTCTATGCATAATAGG+TGG	-	chr8_4:9863417-9863436	None:intergenic	30.0%
ATTCTCATCTAGTAGTATGT+TGG	-	chr8_4:9863448-9863467	None:intergenic	30.0%
TGGTTGTACAAAGATTAAAG+TGG	+	chr8_4:9863744-9863763	Msa1312690:intron	30.0%
CCTAATAGATTTCAAAGAAC+AGG	+	chr8_4:9863885-9863904	Msa1312690:intron	30.0%
ATCAATATTTGGCTAATGTC+TGG	-	chr8_4:9864160-9864179	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	9862539	9864297	9862539	ID=Msa1312690;Name=Msa1312690
chr8_4	mRNA	9862539	9864297	9862539	ID=Msa1312690-mRNA-1;Parent=Msa1312690;Name=Msa1312690-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0\|0\|0\|1\|1\|1\|4\|0\|202
chr8_4	exon	9862539	9862665	9862539	ID=Msa1312690-mRNA-1:exon:3718;Parent=Msa1312690-mRNA-1
chr8_4	exon	9863091	9863153	9863091	ID=Msa1312690-mRNA-1:exon:3719;Parent=Msa1312690-mRNA-1
chr8_4	exon	9863385	9863549	9863385	ID=Msa1312690-mRNA-1:exon:3720;Parent=Msa1312690-mRNA-1
chr8_4	exon	9864044	9864297	9864044	ID=Msa1312690-mRNA-1:exon:3721;Parent=Msa1312690-mRNA-1
chr8_4	CDS	9862539	9862665	9862539	ID=Msa1312690-mRNA-1:cds;Parent=Msa1312690-mRNA-1
chr8_4	CDS	9863091	9863153	9863091	ID=Msa1312690-mRNA-1:cds;Parent=Msa1312690-mRNA-1
chr8_4	CDS	9863385	9863549	9863385	ID=Msa1312690-mRNA-1:cds;Parent=Msa1312690-mRNA-1
chr8_4	CDS	9864044	9864297	9864044	ID=Msa1312690-mRNA-1:cds;Parent=Msa1312690-mRNA-1

Gene Sequence

>Msa1312690

ATGGTAACCTTAATGTTAAACTCCATCGTATTGCTTGCCTTGGTGCTATTTATTTGCATTAGTCAAGCCAATTCAGAACAAGTAGACACACCTGTTTCAAGAGAAATTGCTAACAATGAAAATCCTGTGGAAAATTCAAATATCTTAAACTGGAATGAGAGACTTAACATTGCAGTTGATGCAGCACATGGATTGGATTATCTGCATAATGGATGTAAACCACCTATTATGCATAGAGATTTGAAGCCTTCCAACATACTACTAGATGAGAATTTTCATGCAAAGATTGCTGATTTTGGTTTAAGTAGAGCTTTCGGTAACGACGATGATTCGCATATATCAACACGCCCTGCTGGAGATATTCAAAACATAATGGATACGAGGTTGCAGGGTGAAGTCAGCATCAATTCTGCATGGAAAGTTGTTGAGATAGCCATGTCATGTGTTTCACAAAATGCAGTTGACAGGCCAGACATTAGCCAAATATTGATAGAGCTAAATGAATGTCTATCTTTGGAGATGGTTCAGAGAAATAATGGTAGAGAAAGAGCTGTAGTTGAAATTACGTCTTTAAATGTTATATCACAAACTGCTCCTTTAGCTAGGTAA

Protein sequence

>Msa1312690

MVTLMLNSIVLLALVLFICISQANSEQVDTPVSREIANNENPVENSNILNWNERLNIAVDAAHGLDYLHNGCKPPIMHRDLKPSNILLDENFHAKIADFGLSRAFGNDDDSHISTRPAGDIQNIMDTRLQGEVSINSAWKVVEIAMSCVSQNAVDRPDISQILIELNECLSLEMVQRNNGRERAVVEITSLNVISQTAPLAR*