Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312720 | sp|O64556|Y2923_ARATH | 40.702 | 769 | 358 | 12 | 8 | 680 | 9 | 775 | 0.0 | 551 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312720 | A0A396GEI9 | 80.103 | 774 | 62 | 7 | 1 | 682 | 6 | 779 | 0.0 | 1191 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312720 | MtrunA17_Chr8g0341011 | 80.103 | 774 | 62 | 7 | 1 | 682 | 6 | 779 | 0.0 | 1191 |
| Msa1312720 | MtrunA17_Chr8g0340881 | 68.863 | 774 | 146 | 8 | 3 | 682 | 8 | 780 | 0.0 | 1039 |
| Msa1312720 | MtrunA17_Chr8g0340931 | 67.738 | 778 | 152 | 12 | 1 | 682 | 1 | 775 | 0.0 | 1027 |
| Msa1312720 | MtrunA17_Chr8g0341151 | 66.279 | 774 | 163 | 9 | 1 | 682 | 2 | 769 | 0.0 | 989 |
| Msa1312720 | MtrunA17_Chr8g0340871 | 66.795 | 780 | 158 | 12 | 1 | 682 | 8 | 784 | 0.0 | 987 |
| Msa1312720 | MtrunA17_Chr8g0340891 | 66.366 | 776 | 160 | 16 | 1 | 682 | 6 | 774 | 0.0 | 972 |
| Msa1312720 | MtrunA17_Chr8g0340911 | 64.396 | 778 | 173 | 15 | 1 | 682 | 6 | 775 | 0.0 | 938 |
| Msa1312720 | MtrunA17_Chr8g0347661 | 64.479 | 777 | 180 | 12 | 1 | 682 | 6 | 781 | 0.0 | 933 |
| Msa1312720 | MtrunA17_Chr8g0358831 | 58.365 | 783 | 172 | 16 | 1 | 682 | 14 | 743 | 0.0 | 812 |
| Msa1312720 | MtrunA17_Chr8g0341101 | 46.115 | 785 | 291 | 20 | 21 | 682 | 51 | 826 | 0.0 | 593 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1312720 | AT4G29990.1 | 43.646 | 779 | 333 | 10 | 8 | 682 | 6 | 782 | 0.0 | 602 |
| Msa1312720 | AT2G19230.2 | 40.702 | 769 | 358 | 12 | 8 | 680 | 9 | 775 | 0.0 | 551 |
| Msa1312720 | AT2G19230.1 | 40.649 | 770 | 358 | 12 | 8 | 680 | 9 | 776 | 0.0 | 551 |
| Msa1312720 | AT4G29990.2 | 43.115 | 719 | 303 | 10 | 8 | 622 | 6 | 722 | 0.0 | 543 |
| Msa1312720 | AT2G19210.1 | 40.682 | 762 | 348 | 13 | 18 | 677 | 20 | 779 | 0.0 | 543 |
| Msa1312720 | AT1G05700.1 | 41.357 | 781 | 348 | 18 | 4 | 682 | 6 | 778 | 1.87e-180 | 535 |
| Msa1312720 | AT2G19190.1 | 40.601 | 766 | 354 | 12 | 17 | 682 | 18 | 782 | 1.32e-177 | 529 |
| Msa1312720 | AT1G51880.1 | 41.062 | 772 | 346 | 17 | 1 | 671 | 1 | 764 | 6.13e-177 | 527 |
| Msa1312720 | AT3G46330.1 | 40.492 | 773 | 357 | 18 | 1 | 674 | 1 | 769 | 7.13e-177 | 527 |
| Msa1312720 | AT1G51880.2 | 41.062 | 772 | 346 | 17 | 1 | 671 | 19 | 782 | 1.11e-176 | 527 |
Find 130 sgRNAs with CRISPR-Local
Find 293 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTATTATTGGAGAAGG+AGG | 0.505118 | 8_4:+9950366 | Msa1312720:CDS |
| AAAATGAAGATCAAATTGCG+GGG | 0.674895 | 8_4:-9947061 | None:intergenic |
| AAAATGATATCTACAGCTTT+GGG | 0.378641 | 8_4:+9951737 | Msa1312720:CDS |
| AAAATTCAAATATGTTAAAC+TGG | 0.376064 | 8_4:+9951028 | Msa1312720:CDS |
| AAACAGGTTCACTATCACTG+AGG | 0.702118 | 8_4:+9949369 | Msa1312720:CDS |
| AAAGCATTAGTAAGAGCATC+AGG | 0.425331 | 8_4:+9951790 | Msa1312720:CDS |
| AAAGGGGCGCTAGCATTTAC+TGG | 0.357920 | 8_4:-9947569 | None:intergenic |
| AAAGTCAAGTTGCTGTTAAG+AGG | 0.427381 | 8_4:+9950424 | Msa1312720:CDS |
| AAATCTCTATGCATAATAGG+TGG | 0.593683 | 8_4:-9951197 | None:intergenic |
| AAATGAAGATCAAATTGCGG+GGG | 0.670887 | 8_4:-9947060 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATTTGAGCTATTCTGATA+CGG | + | chr8_4:9946825-9946844 | Msa1312720:CDS | 30.0% | |
| TGGCATCTGATATGTAATTT+AGG | - | chr8_4:9946858-9946877 | None:intergenic | 30.0% | |
| CAAACCAGTATCTATGAATT+TGG | - | chr8_4:9946878-9946897 | None:intergenic | 30.0% | |
| AAAATGAAGATCAAATTGCG+GGG | - | chr8_4:9947064-9947083 | None:intergenic | 30.0% | |
| CAAAATGAAGATCAAATTGC+GGG | - | chr8_4:9947065-9947084 | None:intergenic | 30.0% | |
| CCAAAATGAAGATCAAATTG+CGG | - | chr8_4:9947066-9947085 | None:intergenic | 30.0% | |
| GGTTGTATGTAATCTAATGA+TGG | - | chr8_4:9947159-9947178 | None:intergenic | 30.0% | |
| ATAAACATCATCTTTGTACC+TGG | - | chr8_4:9947426-9947445 | None:intergenic | 30.0% | |
| TCACAGTGAATGATAAGTTA+TGG | + | chr8_4:9947696-9947715 | Msa1312720:CDS | 30.0% | |
| GTGAATGATAAGTTATGGTT+TGG | + | chr8_4:9947701-9947720 | Msa1312720:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 9946594 | 9951846 | 9946594 | ID=Msa1312720;Name=Msa1312720 |
| chr8_4 | mRNA | 9946594 | 9951846 | 9946594 | ID=Msa1312720-mRNA-1;Parent=Msa1312720;Name=Msa1312720-mRNA-1;_AED=0.21;_eAED=0.23;_QI=0|0.27|0.08|0.91|1|1|12|0|689 |
| chr8_4 | exon | 9946594 | 9946675 | 9946594 | ID=Msa1312720-mRNA-1:exon:3736;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9946794 | 9947322 | 9946794 | ID=Msa1312720-mRNA-1:exon:3737;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9947427 | 9947905 | 9947427 | ID=Msa1312720-mRNA-1:exon:3738;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9948793 | 9948847 | 9948793 | ID=Msa1312720-mRNA-1:exon:3739;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9949116 | 9949187 | 9949116 | ID=Msa1312720-mRNA-1:exon:3740;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9949310 | 9949390 | 9949310 | ID=Msa1312720-mRNA-1:exon:3741;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9949861 | 9949920 | 9949861 | ID=Msa1312720-mRNA-1:exon:3742;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9950299 | 9950491 | 9950299 | ID=Msa1312720-mRNA-1:exon:3743;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9950620 | 9950746 | 9950620 | ID=Msa1312720-mRNA-1:exon:3744;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9951025 | 9951087 | 9951025 | ID=Msa1312720-mRNA-1:exon:3745;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9951168 | 9951357 | 9951168 | ID=Msa1312720-mRNA-1:exon:3746;Parent=Msa1312720-mRNA-1 |
| chr8_4 | exon | 9951708 | 9951846 | 9951708 | ID=Msa1312720-mRNA-1:exon:3747;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9946594 | 9946675 | 9946594 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9946794 | 9947322 | 9946794 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9947427 | 9947905 | 9947427 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9948793 | 9948847 | 9948793 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9949116 | 9949187 | 9949116 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9949310 | 9949390 | 9949310 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9949861 | 9949920 | 9949861 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9950299 | 9950491 | 9950299 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9950620 | 9950746 | 9950620 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9951025 | 9951087 | 9951025 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9951168 | 9951357 | 9951168 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| chr8_4 | CDS | 9951708 | 9951846 | 9951708 | ID=Msa1312720-mRNA-1:cds;Parent=Msa1312720-mRNA-1 |
| Gene Sequence |
>Msa1312720 ATGAGAATGTTACTGCATTTTCTTTTAGCATTGCTTGGTGTTCTTACTATCTTGGTTTTGATACAAGCTCAGGATCAATCAGGATTCATTAGCATAGATTGTGGTCTACCAGAACATTTGAGCTATTCTGATACGGACACAGACCTAAATTACATATCAGATGCCAAATTCATAGATACTGGTTTGAGTAAGAGGATACCGTCTACAAACAATGTTCGACGGTATCTAGAATATGTGAGAAGCTTTCCAAGTGGGGTGAGAAATTGTTACAAAATAAACGTAACAAGTGATACTAAATATTTAATCAGAGCTTCCTTCTATTATGGAAACTATGATGGTTTAAATGATCCCCCGCAATTTGATCTTCATTTTGGAGCTAATGTTTGGGATACAGTGAAGTTTCCCAATGCATCACGTATGCGATTCAGTGAGATCATATACACTCCATCATTAGATTACATACAACCATGTCTTGTTAACACAGGATATGGGACTCCGTTCATTTCAGCTATAGAATTAAGGACTTTGAACAATGAAACTTACGTCACATCTTCAGTATTATCACTCTTCAGTCGATGCAATTTAGGTTCCATCACAGACATAGAATACAGGTACAAAGATGATGTTTATGACCGCATGTGGCTTTCTTATGAGTTAATTGATTGGAGACGATTAAGCACCTCATTTAACAATGATGATCTAGTTCAGAATATTTATAAACCACCAACAATTGTGATGAGCACTGCTGCTACACCAGTAAATGCTAGCGCCCCTTTACAATTTCATTGGAATTCAGATAATGTAAATGATCAATACTACCTCTACATGCACTTTAATGAGGTTGAAGAGCTGGCCGTAAATGAAACTAGAGAGTTCAATATCACAGTGAATGATAAGTTATGGTTTGGACCTGTGACGCCTATATACCGGACACCAGATCTCATATTTAGTACAGAACCTTTGCGTAGAGCCGAAACATATCAAATTTCTCTTTCCAAAACAAAGAATTCAACGCTTCCACCCATCCTAAATGCCTTTGAGATTTATATGGCAAAAGACTTCTCACAATCAGAAACTCAACAGGATGATGTTAACCTTCGGATTCTCAGAGAAAAGGGCCCTGGTAATCCAAAGTCGATCTGGAGGGATTTGTCAAACAGTAGTTTAAATGGTCCTTTACCTGATTTTCTGATACAACTGCGATCACTAAAAGTCTTAAACGTGGGGAAGAACAACCTTACTGGATTAGTTCCAGGTGAACTCCTTGAGAGATCAAAAACAGGTTCACTATCACTGAGCAATGATTGTAATCGTCTTGATTTCTCTTGGATTTTGAATATTCAAAAGAAAGACACCAGGGCTTCAACAAAATCAAAACATCAAAGATTCAGTTATACTGAAATTGTCAATATTACGGATAACTTCAAAACTATTATTGGAGAAGGAGGATTTGGAAAAGTTTACTTTGGCATTCTACAAGATCAAAGTCAAGTTGCTGTTAAGAGGCTTTCACCATCATCAATGCAAGGTTATAAGGAATTTCAAGCAGAGGCACAACTTCTAACAGTTGTTCATCATAGAAATTTGGTCTCCCTTGTTGGATATTGTGATGAAGATGAAATCAAAGCATTAATTTATGAATACATGGCCAATGGAAATCTCCAACAACATTTATTAGTGGAAAATTCAAATATGTTAAACTGGAATGAGAGACTTAACATTGCAGTTGATGCAGCACACGGATTGGATTATCTGCATAATGGATGTAAACCACCTATTATGCATAGAGATTTGAAACCTTCAAACATATTACTAGATGAAAACATGCATGCCAAGATTGCTGATTTTGGTCTGAGTAGAGCTTTTGATAATGATATTGATTCTCATATATCCACACGCCCTGCTGGTACATTTGGTTATGTTGATCCAAAATTCCAGAGAACAGGAAACACAAATAAGAAAAATGATATCTACAGCTTTGGGATAGTTCTACTTGAATTGATCACCGGGAAGAAAGCATTAGTAAGAGCATCAGGAGAAAGTGTTGGAACATGTGTGGAAATTGAGTAA |
| Protein sequence |
>Msa1312720 MRMLLHFLLALLGVLTILVLIQAQDQSGFISIDCGLPEHLSYSDTDTDLNYISDAKFIDTGLSKRIPSTNNVRRYLEYVRSFPSGVRNCYKINVTSDTKYLIRASFYYGNYDGLNDPPQFDLHFGANVWDTVKFPNASRMRFSEIIYTPSLDYIQPCLVNTGYGTPFISAIELRTLNNETYVTSSVLSLFSRCNLGSITDIEYRYKDDVYDRMWLSYELIDWRRLSTSFNNDDLVQNIYKPPTIVMSTAATPVNASAPLQFHWNSDNVNDQYYLYMHFNEVEELAVNETREFNITVNDKLWFGPVTPIYRTPDLIFSTEPLRRAETYQISLSKTKNSTLPPILNAFEIYMAKDFSQSETQQDDVNLRILREKGPGNPKSIWRDLSNSSLNGPLPDFLIQLRSLKVLNVGKNNLTGLVPGELLERSKTGSLSLSNDCNRLDFSWILNIQKKDTRASTKSKHQRFSYTEIVNITDNFKTIIGEGGFGKVYFGILQDQSQVAVKRLSPSSMQGYKEFQAEAQLLTVVHHRNLVSLVGYCDEDEIKALIYEYMANGNLQQHLLVENSNMLNWNERLNIAVDAAHGLDYLHNGCKPPIMHRDLKPSNILLDENMHAKIADFGLSRAFDNDIDSHISTRPAGTFGYVDPKFQRTGNTNKKNDIYSFGIVLLELITGKKALVRASGESVGTCVEIE* |
