AlfalfaGEDB | Msa1315910

M. sativa cultivar zhongmu-4 / Msa1315910

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071944 GO:0080090 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315910	sp\|F4JN35\|NTL9_ARATH	58.491	159	57	4	10	162	9	164	2.93e-54	190

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315910	G7ZZI3	87.407	405	45	2	1	404	1	400	0.0	713

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TFs/TRs:

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Gene ID	Type	Classification
Msa1315910	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315910	MtrunA17_Chr8g0345671	87.407	405	45	2	1	404	1	400	0.0	713
Msa1315910	MtrunA17_Chr8g0345691	63.387	437	107	7	1	402	1	419	0.0	515
Msa1315910	MtrunA17_Chr8g0345731	65.132	152	48	3	8	154	22	173	2.11e-65	206
Msa1315910	MtrunA17_Chr8g0345711	62.987	154	54	2	1	151	1	154	1.35e-64	204
Msa1315910	MtrunA17_Chr8g0345721	56.000	175	48	1	1	146	26	200	8.43e-61	196
Msa1315910	MtrunA17_Chr8g0345741	64.493	138	49	0	21	158	2	139	4.11e-60	192
Msa1315910	MtrunA17_Chr3g0128111	55.030	169	63	6	13	175	45	206	1.73e-52	175
Msa1315910	MtrunA17_Chr5g0428991	57.233	159	59	4	8	160	23	178	9.36e-52	183
Msa1315910	MtrunA17_Chr3g0128151	55.556	162	63	5	8	163	20	178	5.07e-51	180
Msa1315910	MtrunA17_Chr8g0345701	56.291	151	48	5	10	146	7	153	1.39e-47	160

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1315910	AT4G35580.3	58.491	159	57	4	10	162	9	164	2.57e-55	191
Msa1315910	AT4G35580.2	58.491	159	57	4	10	162	9	164	2.81e-55	191
Msa1315910	AT4G35580.1	58.491	159	57	4	10	162	9	164	2.98e-55	190
Msa1315910	AT1G33060.1	57.143	154	57	4	10	157	24	174	1.91e-51	182
Msa1315910	AT1G33060.2	57.143	154	57	4	10	157	24	174	2.14e-51	182
Msa1315910	AT4G01550.1	43.805	226	108	7	12	235	6	214	3.93e-51	178
Msa1315910	AT3G49530.2	53.086	162	68	4	10	165	13	172	7.68e-51	176
Msa1315910	AT3G49530.1	53.086	162	68	4	10	165	13	172	3.58e-50	176
Msa1315910	AT5G24590.2	53.205	156	65	4	10	159	13	166	7.65e-49	172
Msa1315910	AT3G17730.1	49.102	167	76	4	10	170	6	169	1.80e-45	157

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Find 64 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACTTGTGTACTATAA+TGG	0.357000	8_4:+15493843	Msa1315910:CDS
AACCTGGTTATCATCAAAGG+TGG	0.663139	8_4:-15493916	None:intergenic
AAGCATCTTTATCGAACTCA+GGG	0.557608	8_4:+15494372	Msa1315910:CDS
AATCTTACGGTCAAGACCAG+TGG	0.626820	8_4:-15493784	None:intergenic
ACAAACCTGGTTATCATCAA+AGG	0.564326	8_4:-15493919	None:intergenic
ACAAATACGCAAATAGTAAA+AGG	0.269504	8_4:+15493727	Msa1315910:CDS
ACCCAGTTGCTATTAACACC+AGG	0.638889	8_4:-15493873	None:intergenic
AGAGGTACTGAAAATGTTAT+TGG	0.269464	8_4:+15493813	Msa1315910:CDS
AGCTATAACATTGTTGACAG+TGG	0.605859	8_4:-15493528	None:intergenic
ATAGAATGATATGTGATGAA+GGG	0.590150	8_4:+15494086	Msa1315910:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGACTAAAGAAAAACCATT+CGG	-	chr8_4:15493700-15493719	None:intergenic	30.0%
TACCTCTAACCTTAATCTTA+CGG	-	chr8_4:15493800-15493819	None:intergenic	30.0%
TTGTTGTAACAAAACAAACC+TGG	-	chr8_4:15493935-15493954	None:intergenic	30.0%
GATAGAATGATATGTGATGA+AGG	+	chr8_4:15494085-15494104	Msa1315910:CDS	30.0%
TAGAATGATATGTGATGAAG+GGG	+	chr8_4:15494087-15494106	Msa1315910:CDS	30.0%
GAAAATCATGAAACAGTTGA+AGG	+	chr8_4:15494139-15494158	Msa1315910:CDS	30.0%
AAGAATGAAATCCTTACATC+AGG	-	chr8_4:15494169-15494188	None:intergenic	30.0%
ACTTGTTTGTCAATTGTTTC+AGG	+	chr8_4:15494232-15494251	Msa1315910:intron	30.0%
TCGTTGAGAATGATTTACTA+TGG	+	chr8_4:15494566-15494585	Msa1315910:CDS	30.0%
AGGACTAAAGAAAAACCATT+CGG	-	chr8_4:15493700-15493719	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	15493419	15495010	15493419	ID=Msa1315910;Name=Msa1315910
chr8_4	mRNA	15493419	15495010	15493419	ID=Msa1315910-mRNA-1;Parent=Msa1315910;Name=Msa1315910-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0\|0.5\|0\|1\|1\|1\|5\|0\|404
chr8_4	exon	15493419	15493590	15493419	ID=Msa1315910-mRNA-1:exon:5983;Parent=Msa1315910-mRNA-1
chr8_4	exon	15493670	15493935	15493670	ID=Msa1315910-mRNA-1:exon:5984;Parent=Msa1315910-mRNA-1
chr8_4	exon	15494025	15494171	15494025	ID=Msa1315910-mRNA-1:exon:5985;Parent=Msa1315910-mRNA-1
chr8_4	exon	15494254	15494625	15494254	ID=Msa1315910-mRNA-1:exon:5986;Parent=Msa1315910-mRNA-1
chr8_4	exon	15494753	15495010	15494753	ID=Msa1315910-mRNA-1:exon:5987;Parent=Msa1315910-mRNA-1
chr8_4	CDS	15493419	15493590	15493419	ID=Msa1315910-mRNA-1:cds;Parent=Msa1315910-mRNA-1
chr8_4	CDS	15493670	15493935	15493670	ID=Msa1315910-mRNA-1:cds;Parent=Msa1315910-mRNA-1
chr8_4	CDS	15494025	15494171	15494025	ID=Msa1315910-mRNA-1:cds;Parent=Msa1315910-mRNA-1
chr8_4	CDS	15494254	15494625	15494254	ID=Msa1315910-mRNA-1:cds;Parent=Msa1315910-mRNA-1
chr8_4	CDS	15494753	15495010	15494753	ID=Msa1315910-mRNA-1:cds;Parent=Msa1315910-mRNA-1

Gene Sequence

>Msa1315910

ATGACAAACTCATTTCCTCATAACTATGTTCCTGTTGGATTCAGATTCCGACCAACAGATGAAGAGCTTGTAAACCACTATCTCAAAAACAAGTTATTTGGTAATGATTCCACTGTCAACAATGTTATAGCTGAAGTTGATGTTTGCAAGTTCGAACCTTGGGACTTACCAGCTTGTTCAATCATAAAATCCGATGATCCCGAATGGTTTTTCTTTAGTCCTCGTGATTACAAATACGCAAATAGTAAAAGGTTTAACAGAAGGACACAGTGTGGATACTGGAAAGCCACTGGTCTTGACCGTAAGATTAAGGTTAGAGGTACTGAAAATGTTATTGGGACAAAGAAAACACTTGTGTACTATAATGGTTGTGCTCCTGGTGTTAATAGCAACTGGGTGATTCATGAATATCATTCTACCACCTTTGATGATAACCAGAGGACTTTTGTTTTGTGTCGCTTGATGAAGAAAGCCGAGAAAAAAACTGAAGAGGAAACTGATAGAATGATATGTGATGAAGGGGAGCCAAGTAGACACATGAGTTCCGATCACGAAAATCATGAAACAGTTGAAGGAATTCCTGATGTAATAAGTGGTACCTCGCCAGAAAAGAATATGGAACCAATGTTTCAGGAACCTCATCAAGCCGAAAAGTACTTTCCTTTGTCAACATACCAGTCTTCAATCTCTGAAAATGAGATGGAAGCATCTTTATCGAACTCAGGGTTTTATGATTCCGAAAGTGGACACATCGATATGCAGAGTCCGTTCGAAACTATTGATGAGGAAAATCAGTTCATCAATTCAATGTTAAATGACGGATATTTTGTTAAGGATAATGATATGTATAGCGAACAAAGAAGACACACTTTTGCCAGCTTTCCTGTCCAGTCCGAGTCGTTGAGAATGATTTACTATGGAAGCAGTGACACAAATGCTGAATTAGTCTCTATGCCATGTGGCAATGTTCTGGATACTTCAACGGCGCTTCTTGAACATCCTAGTTCAGGCGAATACTATGCATCATTTCGAGATGATTTCGGGGGATTTGAGGCATCATCTTGTGATTCAACTGCAGATAAACCTCTAGAGATGATCAATTGCAATGAAATTTCTAGTGCACCTTCCACTCTACGAAGAAACAATAACCAGTATCATCCAAGACCAACCACCACCATGTCAAAAAGATCTGCTTCAAGACGATCTCAAACATAG

Protein sequence

>Msa1315910

MTNSFPHNYVPVGFRFRPTDEELVNHYLKNKLFGNDSTVNNVIAEVDVCKFEPWDLPACSIIKSDDPEWFFFSPRDYKYANSKRFNRRTQCGYWKATGLDRKIKVRGTENVIGTKKTLVYYNGCAPGVNSNWVIHEYHSTTFDDNQRTFVLCRLMKKAEKKTEEETDRMICDEGEPSRHMSSDHENHETVEGIPDVISGTSPEKNMEPMFQEPHQAEKYFPLSTYQSSISENEMEASLSNSGFYDSESGHIDMQSPFETIDEENQFINSMLNDGYFVKDNDMYSEQRRHTFASFPVQSESLRMIYYGSSDTNAELVSMPCGNVLDTSTALLEHPSSGEYYASFRDDFGGFEASSCDSTADKPLEMINCNEISSAPSTLRRNNNQYHPRPTTTMSKRSASRRSQT*