AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1319730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1319730 MtrunA17_Chr8g0350761 92.000 475 33 2 1 475 1 470 0.0 898
Msa1319730 MtrunA17_Chr4g0067981 31.295 278 152 7 20 287 33 281 5.17e-26 111
Msa1319730 MtrunA17_Chr3g0123131 30.620 258 153 8 20 272 15 251 6.65e-25 107
Msa1319730 MtrunA17_Chr7g0275401 27.305 282 186 5 21 300 8 272 1.88e-24 103
Msa1319730 MtrunA17_Chr8g0376881 30.709 254 156 9 20 272 27 261 3.64e-24 105
Msa1319730 MtrunA17_Chr1g0161431 29.961 257 159 5 20 272 13 252 2.90e-23 103
Msa1319730 MtrunA17_Chr3g0137101 27.734 256 165 6 21 272 11 250 4.18e-22 99.8
Msa1319730 MtrunA17_Chr2g0294911 27.881 269 172 5 20 287 31 278 3.15e-21 97.1
Msa1319730 MtrunA17_Chr4g0074401 27.626 257 168 5 20 275 19 258 7.58e-21 95.9
Msa1319730 MtrunA17_Chr4g0074411 26.538 260 162 4 21 275 21 256 1.43e-20 94.7
Msa1319730 MtrunA17_Chr1g0149841 28.352 261 153 6 21 272 8 243 2.99e-20 93.6
Msa1319730 MtrunA17_Chr7g0275891 24.830 294 174 7 21 292 8 276 6.26e-18 85.1
Msa1319730 MtrunA17_Chr1g0149851 27.046 281 168 6 21 283 8 269 3.98e-17 84.0
Msa1319730 MtrunA17_Chr4g0074421 26.066 211 115 6 69 269 3 182 8.69e-14 70.1
Msa1319730 MtrunA17_Chr2g0304841 30.252 119 82 1 21 139 21 138 6.30e-13 71.2
Msa1319730 MtrunA17_Chr6g0456631 34.426 122 76 3 20 139 11 130 4.25e-12 64.7
Msa1319730 MtrunA17_Chr3g0134481 30.328 122 84 1 21 142 11 131 3.28e-11 62.4
Msa1319730 MtrunA17_Chr8g0383961 32.258 124 80 2 22 142 18 140 5.81e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1319730 AT3G16857.1 27.106 273 186 6 20 285 35 301 1.61e-23 104
Msa1319730 AT3G16857.2 27.106 273 186 6 20 285 35 301 1.64e-23 104
Msa1319730 AT2G25180.1 27.586 261 151 6 21 272 16 247 1.52e-21 98.2
Msa1319730 AT4G31920.1 26.172 256 155 4 21 275 16 238 4.13e-20 93.6
Msa1319730 AT1G67710.1 28.462 260 157 8 20 272 9 246 5.76e-20 93.2
Msa1319730 AT5G58080.1 27.138 269 171 8 5 272 2 246 1.23e-18 89.4
Msa1319730 AT1G49190.1 24.908 273 176 9 20 285 32 282 5.58e-16 80.1
Msa1319730 AT3G16857.3 25.424 236 163 6 57 285 2 231 6.62e-16 80.9
Msa1319730 AT2G01760.1 35.537 121 76 2 20 139 9 128 5.73e-15 77.0
Msa1319730 AT5G49240.1 23.019 265 155 8 21 269 41 272 6.95e-13 69.7
Msa1319730 AT2G40970.1 33.333 135 73 3 219 351 103 222 5.22e-11 63.5
Msa1319730 AT4G00760.1 28.571 203 136 5 40 238 1 198 6.95e-11 63.9

Find 109 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTAGACCAATTGCATTT+TGG 0.197316 8_4:+23494771 None:intergenic
ATATCATGGGCTCTTGGTTT+TGG 0.200822 8_4:-23494986 Msa1319730:CDS
CTATGATCTCATAGGGAAAA+TGG 0.221957 8_4:-23495173 Msa1319730:CDS
GAGTGCCTCAAACTATTTAC+AGG 0.272286 8_4:-23494438 Msa1319730:intron
GTTGTCCGGAGTCACCTTTC+AGG 0.273076 8_4:-23493881 Msa1319730:CDS
ATTCTTCTTCTCACAGATAA+TGG 0.274227 8_4:+23495243 None:intergenic
TGATTTACATATGAAGTTCT+TGG 0.275930 8_4:-23494630 Msa1319730:CDS
AGTACTGAGTTATCTTTATC+AGG 0.287313 8_4:+23494683 None:intergenic
ATAGTTTGAGGCACTCTCTT+TGG 0.318103 8_4:+23494445 None:intergenic
TTCATGGATGGTTGGCATTT+GGG 0.333819 8_4:+23494812 None:intergenic
ATTCATGGATGGTTGGCATT+TGG 0.334762 8_4:+23494811 None:intergenic
ATGGAAATCTATGATCTCAT+AGG 0.353225 8_4:-23495181 Msa1319730:CDS
AGATCCTTCGGATCGGCATC+TGG 0.369128 8_4:+23493969 None:intergenic
ACCCTTCCACATTCATAATC+TGG 0.374739 8_4:+23494471 None:intergenic
TATATCATTGTGCGTGCGCT+CGG 0.375322 8_4:-23493512 Msa1319730:CDS
TGGCTTTCTAAAACACCATT+TGG 0.375414 8_4:+23494961 None:intergenic
CTTCTTTCACTTGGATATGA+AGG 0.378032 8_4:-23495800 Msa1319730:intron
AAAACCAAGAGCCCATGATA+TGG 0.384656 8_4:+23494988 None:intergenic
CAATGACTGTTGTTTCATAT+CGG 0.387086 8_4:+23494891 None:intergenic
GCATCTGTCAGATGTGATTA+TGG 0.389198 8_4:-23494846 Msa1319730:CDS
CCTTCCATATCATGGGCTCT+TGG 0.394328 8_4:-23494992 Msa1319730:CDS
CACGCACAATGATATATTAC+TGG 0.397475 8_4:+23493519 None:intergenic
TCCTCAGCTTCGCCGCTGAC+TGG 0.399441 8_4:+23495903 None:intergenic
AAACCATTCTCCTGTATTCA+TGG 0.400145 8_4:+23494796 None:intergenic
CTCCTGCATTCAAAGATGGT+TGG 0.406146 8_4:+23494747 None:intergenic
TGGTTGGCATTTGGGACCCT+TGG 0.407552 8_4:+23494820 None:intergenic
TTTCTGATCTACATGACCTT+TGG 0.412759 8_4:-23494931 Msa1319730:CDS
CAAAATGCAATTGGTCTAGA+AGG 0.418166 8_4:-23494770 Msa1319730:CDS
ATAACTCAGTACTCCTGAAC+AGG 0.440547 8_4:-23494673 Msa1319730:CDS
CAGTACTCCTGAACAGGAAA+AGG 0.443080 8_4:-23494667 Msa1319730:CDS
CTTGGAAAGCTCAAGTTGTC+CGG 0.445175 8_4:-23493895 Msa1319730:CDS
GACAGATGCCACACGAAGTC+AGG 0.457217 8_4:+23494860 None:intergenic
CTCCTGTATTCATGGATGGT+TGG 0.460192 8_4:+23494804 None:intergenic
ACGACACTCCTTCCATATCA+TGG 0.461900 8_4:-23495000 Msa1319730:CDS
TATGATCTCATAGGGAAAAT+GGG 0.462257 8_4:-23495172 Msa1319730:CDS
GTTGGTTGTGGATGCTAGTT+CGG 0.466592 8_4:-23495849 Msa1319730:CDS
AAATCAGCTGTCTCTGCCAT+TGG 0.467045 8_4:-23493856 Msa1319730:CDS
TTGGAAGCTGTTGAAATAGC+AGG 0.471879 8_4:-23494611 Msa1319730:CDS
TGAACAGGAAAAGGAAAAGC+TGG 0.472904 8_4:-23494658 Msa1319730:CDS
TGGTTTCGCCAAAATGCAAT+TGG 0.475231 8_4:-23494779 Msa1319730:CDS
AAGTGCAAATGGTAAAGAAC+AGG 0.480421 8_4:-23494711 Msa1319730:CDS
AGATCATAGATTTCCATGTC+TGG 0.480523 8_4:+23495187 None:intergenic
ATATCATTGTGCGTGCGCTC+GGG 0.484893 8_4:-23493511 Msa1319730:CDS
TGTAGATCAGAAATCTGAAC+TGG 0.485157 8_4:+23494941 None:intergenic
TCCAGTCAGCGGCGAAGCTG+AGG 0.492869 8_4:-23495904 Msa1319730:CDS
AAGACTCTCCGAAGTGCAAA+TGG 0.496589 8_4:-23494722 Msa1319730:CDS
TGCCAACCATCCATGAATAC+AGG 0.500767 8_4:-23494806 Msa1319730:CDS
ACAAACCTGTAAATAGTTTG+AGG 0.502869 8_4:+23494433 None:intergenic
CATCTGCTGTATTCAAAACC+TGG 0.512978 8_4:+23493751 None:intergenic
TCTGGTGGTAGTGGCGGCAT+AGG 0.521933 8_4:+23493645 None:intergenic
ATAGGAAGCACCATGCATAA+TGG 0.522991 8_4:+23493663 None:intergenic
GGCCAACCATCTTTGAATGC+AGG 0.525238 8_4:-23494749 Msa1319730:CDS
GATGGAGAAGTTTCTAATGC+AGG 0.527317 8_4:-23493732 Msa1319730:CDS
TTCCAGATTATGAATGTGGA+AGG 0.529710 8_4:-23494473 Msa1319730:CDS
GAACCTACGTGCTACTGCAA+TGG 0.529949 8_4:-23494075 Msa1319730:CDS
CATTCTCCTGTATTCATGGA+TGG 0.530842 8_4:+23494800 None:intergenic
GTTCTTTACCATTTGCACTT+CGG 0.531052 8_4:+23494714 None:intergenic
AACTCAGCTGTTTACTGATG+AGG 0.532043 8_4:-23493583 Msa1319730:CDS
CGATCCAGATGCCGATCCGA+AGG 0.532999 8_4:-23493973 Msa1319730:CDS
GGAGGTTCAGTTGCCAGACA+TGG 0.533668 8_4:-23495200 Msa1319730:CDS
ACAAAATTACTGAGATCCTT+CGG 0.535642 8_4:+23493957 None:intergenic
AGGTACCATGGTCCAGTAGC+CGG 0.537071 8_4:-23495881 Msa1319730:CDS
TGAGGTTAACCTTGTGCTTG+TGG 0.538862 8_4:-23495221 Msa1319730:CDS
TGGAAATCTATGATCTCATA+GGG 0.539851 8_4:-23495180 Msa1319730:CDS
GTTTATAAACCTACCAAGGC+TGG 0.543151 8_4:+23493806 None:intergenic
ACTAGAACTTACTGAAAGAC+GGG 0.545088 8_4:+23495134 None:intergenic
GCGCACACCACATCCAGCCT+TGG 0.545507 8_4:-23493819 Msa1319730:CDS
TCTTTCAATACTAGTTATGA+CGG 0.548526 8_4:-23495290 Msa1319730:intron
CGGACAACTTGAGCTTTCCA+AGG 0.549307 8_4:+23493896 None:intergenic
GCAGGACTTTGCTGTTCCAA+TGG 0.549979 8_4:+23493840 None:intergenic
AAACCTACCAAGGCTGGATG+TGG 0.553582 8_4:+23493812 None:intergenic
TACTAGAACTTACTGAAAGA+CGG 0.553968 8_4:+23495133 None:intergenic
CTGATGAGGATTTGAAAATG+TGG 0.559224 8_4:-23493569 Msa1319730:CDS
AATATTCCAGATTATGAATG+TGG 0.565218 8_4:-23494477 Msa1319730:CDS
CGACACTCCTTCCATATCAT+GGG 0.565781 8_4:-23494999 Msa1319730:CDS
AGGCTGGATGTGGTGTGCGC+AGG 0.568764 8_4:+23493822 None:intergenic
CATCCATGAATACAGGAGAA+TGG 0.569815 8_4:-23494799 Msa1319730:CDS
AGTCAGCGGCGAAGCTGAGG+AGG 0.585284 8_4:-23495901 Msa1319730:CDS
ACACCACATCCAGCCTTGGT+AGG 0.587197 8_4:-23493815 Msa1319730:CDS
TCCACACTGCGAATCGCCAA+AGG 0.589805 8_4:+23494915 None:intergenic
TCCAGATTATGAATGTGGAA+GGG 0.595446 8_4:-23494472 Msa1319730:CDS
CAGATACATCATCATTAACA+CGG 0.604526 8_4:+23493784 None:intergenic
GGACAACTTGAGCTTTCCAA+GGG 0.606529 8_4:+23493897 None:intergenic
TCTCGGACTAACAAAAGTCA+AGG 0.607987 8_4:-23494029 Msa1319730:CDS
AGATGTGATTATGGCAACCA+AGG 0.609212 8_4:-23494837 Msa1319730:CDS
ACCTTTGGCGATTCGCAGTG+TGG 0.611049 8_4:-23494916 Msa1319730:CDS
GTTGAAATAGCAGGAATCGA+TGG 0.612564 8_4:-23494602 Msa1319730:intron
ATGTGGTTATCAACGATTCG+AGG 0.613048 8_4:-23493552 Msa1319730:CDS
CACGGTTTATAAACCTACCA+AGG 0.615074 8_4:+23493802 None:intergenic
CCAAGAGCCCATGATATGGA+AGG 0.615756 8_4:+23494992 None:intergenic
TTACACCGGCTACTGGACCA+TGG 0.619788 8_4:+23495876 None:intergenic
ACAGATAATGGATAATGCAT+CGG 0.619908 8_4:+23495255 None:intergenic
AGTAGCCGGTGTAAAAGTGT+TGG 0.620685 8_4:-23495867 Msa1319730:CDS
GGTTAACCTTGTGCTTGTGG+AGG 0.621345 8_4:-23495218 Msa1319730:CDS
TGTATCTGAAAAGATCAGCC+AGG 0.622080 8_4:-23493769 Msa1319730:CDS
GATGTGATTATGGCAACCAA+GGG 0.624431 8_4:-23494836 Msa1319730:CDS
GAAGTGTCAAGAATATGCAC+AGG 0.635227 8_4:+23493933 None:intergenic
GCGAAGCTGAGGAGGTACCA+TGG 0.649372 8_4:-23495893 Msa1319730:CDS
CGGTGTAAAAGTGTTGGTTG+TGG 0.652064 8_4:-23495861 Msa1319730:CDS
AAGCAGTACCTGACTTCGTG+TGG 0.652087 8_4:-23494868 Msa1319730:CDS
TTCTGACGAGTTCCAGTCAG+CGG 0.660383 8_4:-23495915 Msa1319730:CDS
ATGTGAATGAATATGTGTCT+CGG 0.664181 8_4:-23494046 Msa1319730:CDS
ACTGAACCTCCACAAGCACA+AGG 0.665135 8_4:+23495212 None:intergenic
ATTACTGAGATCCTTCGGAT+CGG 0.667987 8_4:+23493962 None:intergenic
TGTAGACTCACTCGTGCTCG+CGG 0.670245 8_4:+23493488 None:intergenic
GTTCCATTGCAGTAGCACGT+AGG 0.677485 8_4:+23494072 None:intergenic
CATGTGCGAACCATTATGCA+TGG 0.704435 8_4:-23493673 Msa1319730:CDS
TATCTGTGAGAAGAAGAATG+AGG 0.710608 8_4:-23495239 Msa1319730:CDS
TCGCACATGTCAAAGCTCAG+CGG 0.744624 8_4:+23493687 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTTCAAAAATAAATAAAAA+TGG + chr8_4:23494075-23494094 None:intergenic 10.0%
!! ATCAATGTGATTATTCATTA+TGG - chr8_4:23493770-23493789 Msa1319730:CDS 20.0%
!!! TTTTCAATTTTTGCTTCTTA+AGG - chr8_4:23494027-23494046 Msa1319730:CDS 20.0%
!! AAACAATCAAATGTACTATT+AGG + chr8_4:23495284-23495303 None:intergenic 20.0%
!! GCATTTTTGATACAATTGTT+CGG + chr8_4:23493580-23493599 None:intergenic 25.0%
!! TGGAAATATCTGAAACAATA+AGG + chr8_4:23493717-23493736 None:intergenic 25.0%
!!! TTTATCATATTCATTGACTC+TGG - chr8_4:23493809-23493828 Msa1319730:CDS 25.0%
! GAAGCAAAAATTGAAAATCA+CGG + chr8_4:23494024-23494043 None:intergenic 25.0%
! TCTTTCAATACTAGTTATGA+CGG - chr8_4:23494109-23494128 Msa1319730:intron 25.0%
! TGATTTACATATGAAGTTCT+TGG - chr8_4:23494769-23494788 Msa1319730:CDS 25.0%
! AATATTCCAGATTATGAATG+TGG - chr8_4:23494922-23494941 Msa1319730:CDS 25.0%
!! TTTTTAATAACCGATATGTG+TGG - chr8_4:23495040-23495059 Msa1319730:intron 25.0%
!! TAAAGTCTGAAACTCAAAAT+AGG - chr8_4:23495089-23495108 Msa1319730:intron 25.0%
!!! AATTTCAGTTTTGTTTTAGC+AGG - chr8_4:23495191-23495210 Msa1319730:CDS 25.0%
!!! TCAAAACTTGTTTTTCTAGA+GGG + chr8_4:23495402-23495421 None:intergenic 25.0%
!!! ATCAAAACTTGTTTTTCTAG+AGG + chr8_4:23495403-23495422 None:intergenic 25.0%
TCAAAAATGCTTCTTTCACT+TGG - chr8_4:23493590-23493609 Msa1319730:CDS 30.0%
AAGGTAATTGACACTTATTC+TGG - chr8_4:23493618-23493637 Msa1319730:CDS 30.0%
CTTCTTAAGGCTAAACTATT+GGG - chr8_4:23494040-23494059 Msa1319730:CDS 30.0%
!! ACAGATAATGGATAATGCAT+CGG + chr8_4:23494147-23494166 None:intergenic 30.0%
ATTCTTCTTCTCACAGATAA+TGG + chr8_4:23494159-23494178 None:intergenic 30.0%
ATGGAAATCTATGATCTCAT+AGG - chr8_4:23494218-23494237 Msa1319730:intron 30.0%
TGGAAATCTATGATCTCATA+GGG - chr8_4:23494219-23494238 Msa1319730:intron 30.0%
TATGATCTCATAGGGAAAAT+GGG - chr8_4:23494227-23494246 Msa1319730:intron 30.0%
TACTAGAACTTACTGAAAGA+CGG + chr8_4:23494269-23494288 None:intergenic 30.0%
!! CAATGACTGTTGTTTCATAT+CGG + chr8_4:23494511-23494530 None:intergenic 30.0%
! AGTACTGAGTTATCTTTATC+AGG + chr8_4:23494719-23494738 None:intergenic 30.0%
ACAAACCTGTAAATAGTTTG+AGG + chr8_4:23494969-23494988 None:intergenic 30.0%
! TACAGGTTTGTTCTTTGTAA+TGG - chr8_4:23494978-23494997 Msa1319730:CDS 30.0%
ATGTGAATGAATATGTGTCT+CGG - chr8_4:23495353-23495372 Msa1319730:intron 30.0%
ACAAAATTACTGAGATCCTT+CGG + chr8_4:23495445-23495464 None:intergenic 30.0%
CAGATACATCATCATTAACA+CGG + chr8_4:23495618-23495637 None:intergenic 30.0%
!!! TCATAGTATGCTTTTTCATC+TGG + chr8_4:23495775-23495794 None:intergenic 30.0%
CTTCTTTCACTTGGATATGA+AGG - chr8_4:23493599-23493618 Msa1319730:CDS 35.0%
AGATACAACAGCTAGAAATG+TGG + chr8_4:23493737-23493756 None:intergenic 35.0%
TTGTATGATGATCTTAAGCG+AGG + chr8_4:23493878-23493897 None:intergenic 35.0%
GCTTCTTAAGGCTAAACTAT+TGG - chr8_4:23494039-23494058 Msa1319730:CDS 35.0%
TATCTGTGAGAAGAAGAATG+AGG - chr8_4:23494160-23494179 Msa1319730:intron 35.0%
AGATCATAGATTTCCATGTC+TGG + chr8_4:23494215-23494234 None:intergenic 35.0%
CTATGATCTCATAGGGAAAA+TGG - chr8_4:23494226-23494245 Msa1319730:intron 35.0%
ACTAGAACTTACTGAAAGAC+GGG + chr8_4:23494268-23494287 None:intergenic 35.0%
! GTAAGTTCTAGTACTAGCTT+TGG - chr8_4:23494276-23494295 Msa1319730:intron 35.0%
!! TGGCTTTCTAAAACACCATT+TGG + chr8_4:23494441-23494460 None:intergenic 35.0%
TGTAGATCAGAAATCTGAAC+TGG + chr8_4:23494461-23494480 None:intergenic 35.0%
TTTCTGATCTACATGACCTT+TGG - chr8_4:23494468-23494487 Msa1319730:CDS 35.0%
AAACCATTCTCCTGTATTCA+TGG + chr8_4:23494606-23494625 None:intergenic 35.0%
! CTTCTAGACCAATTGCATTT+TGG + chr8_4:23494631-23494650 None:intergenic 35.0%
! CAAAATGCAATTGGTCTAGA+AGG - chr8_4:23494629-23494648 Msa1319730:CDS 35.0%
! TTTTCTCCTGCATTCAAAGA+TGG + chr8_4:23494659-23494678 None:intergenic 35.0%
GTTCTTTACCATTTGCACTT+CGG + chr8_4:23494688-23494707 None:intergenic 35.0%
AAGTGCAAATGGTAAAGAAC+AGG - chr8_4:23494688-23494707 Msa1319730:CDS 35.0%
TTCCAGATTATGAATGTGGA+AGG - chr8_4:23494926-23494945 Msa1319730:CDS 35.0%
TCCAGATTATGAATGTGGAA+GGG - chr8_4:23494927-23494946 Msa1319730:CDS 35.0%
! TGGACATTTTCCACACATAT+CGG + chr8_4:23495053-23495072 None:intergenic 35.0%
AAACTCAAAATAGGCACACT+TGG - chr8_4:23495098-23495117 Msa1319730:intron 35.0%
AACTCAAAATAGGCACACTT+GGG - chr8_4:23495099-23495118 Msa1319730:intron 35.0%
!! AACTTTTTAGTAATCGCCAC+TGG + chr8_4:23495148-23495167 None:intergenic 35.0%
!! TCTGTTTCTGCATTATCATG+TGG - chr8_4:23495244-23495263 Msa1319730:CDS 35.0%
TGTACTATTAGGTCGTGATT+AGG + chr8_4:23495273-23495292 None:intergenic 35.0%
!!! TAGTATGCTTTTTCATCTGG+TGG + chr8_4:23495772-23495791 None:intergenic 35.0%
CTGATGAGGATTTGAAAATG+TGG - chr8_4:23495830-23495849 Msa1319730:CDS 35.0%
CACGCACAATGATATATTAC+TGG + chr8_4:23495883-23495902 None:intergenic 35.0%
! CACAACCAACACTTTTACAC+CGG + chr8_4:23493540-23493559 None:intergenic 40.0%
AAAACCAAGAGCCCATGATA+TGG + chr8_4:23494414-23494433 None:intergenic 40.0%
! ATATCATGGGCTCTTGGTTT+TGG - chr8_4:23494413-23494432 Msa1319730:intron 40.0%
GCATCTGTCAGATGTGATTA+TGG - chr8_4:23494553-23494572 Msa1319730:intron 40.0%
AGATGTGATTATGGCAACCA+AGG - chr8_4:23494562-23494581 Msa1319730:intron 40.0%
GATGTGATTATGGCAACCAA+GGG - chr8_4:23494563-23494582 Msa1319730:intron 40.0%
TTCATGGATGGTTGGCATTT+GGG + chr8_4:23494590-23494609 None:intergenic 40.0%
ATTCATGGATGGTTGGCATT+TGG + chr8_4:23494591-23494610 None:intergenic 40.0%
CATTCTCCTGTATTCATGGA+TGG + chr8_4:23494602-23494621 None:intergenic 40.0%
CATCCATGAATACAGGAGAA+TGG - chr8_4:23494600-23494619 Msa1319730:intron 40.0%
TGGTTTCGCCAAAATGCAAT+TGG - chr8_4:23494620-23494639 Msa1319730:CDS 40.0%
ATAACTCAGTACTCCTGAAC+AGG - chr8_4:23494726-23494745 Msa1319730:CDS 40.0%
! AGCTTTTCCTTTTCCTGTTC+AGG + chr8_4:23494742-23494761 None:intergenic 40.0%
TGAACAGGAAAAGGAAAAGC+TGG - chr8_4:23494741-23494760 Msa1319730:CDS 40.0%
TTGGAAGCTGTTGAAATAGC+AGG - chr8_4:23494788-23494807 Msa1319730:CDS 40.0%
GTTGAAATAGCAGGAATCGA+TGG - chr8_4:23494797-23494816 Msa1319730:CDS 40.0%
TTGTCTTTCTTGTGTGAAGC+TGG - chr8_4:23494890-23494909 Msa1319730:CDS 40.0%
ACCCTTCCACATTCATAATC+TGG + chr8_4:23494931-23494950 None:intergenic 40.0%
! ATAGTTTGAGGCACTCTCTT+TGG + chr8_4:23494957-23494976 None:intergenic 40.0%
GAGTGCCTCAAACTATTTAC+AGG - chr8_4:23494961-23494980 Msa1319730:CDS 40.0%
TTTACATCAAGCAGTACGCT+TGG + chr8_4:23495073-23495092 None:intergenic 40.0%
ACTCAAAATAGGCACACTTG+GGG - chr8_4:23495100-23495119 Msa1319730:intron 40.0%
! TTTTAGTAATCGCCACTGGT+GGG + chr8_4:23495144-23495163 None:intergenic 40.0%
!! TTTTTAGTAATCGCCACTGG+TGG + chr8_4:23495145-23495164 None:intergenic 40.0%
TCTCGGACTAACAAAAGTCA+AGG - chr8_4:23495370-23495389 Msa1319730:intron 40.0%
ATTACTGAGATCCTTCGGAT+CGG + chr8_4:23495440-23495459 None:intergenic 40.0%
! GAAGTGTCAAGAATATGCAC+AGG + chr8_4:23495469-23495488 None:intergenic 40.0%
!!! TTCTGCTACTTTTCTTCCCT+TGG - chr8_4:23495486-23495505 Msa1319730:intron 40.0%
GTTTATAAACCTACCAAGGC+TGG + chr8_4:23495596-23495615 None:intergenic 40.0%
CACGGTTTATAAACCTACCA+AGG + chr8_4:23495600-23495619 None:intergenic 40.0%
TGTATCTGAAAAGATCAGCC+AGG - chr8_4:23495630-23495649 Msa1319730:intron 40.0%
CATCTGCTGTATTCAAAACC+TGG + chr8_4:23495651-23495670 None:intergenic 40.0%
!!! CAGGTTTTGAATACAGCAGA+TGG - chr8_4:23495649-23495668 Msa1319730:intron 40.0%
GATGGAGAAGTTTCTAATGC+AGG - chr8_4:23495667-23495686 Msa1319730:intron 40.0%
!! ATAGGAAGCACCATGCATAA+TGG + chr8_4:23495739-23495758 None:intergenic 40.0%
AACTCAGCTGTTTACTGATG+AGG - chr8_4:23495816-23495835 Msa1319730:CDS 40.0%
ATGTGGTTATCAACGATTCG+AGG - chr8_4:23495847-23495866 Msa1319730:CDS 40.0%
!! AACACTTTTACACCGGCTAC+TGG + chr8_4:23493533-23493552 None:intergenic 45.0%
! AGTAGCCGGTGTAAAAGTGT+TGG - chr8_4:23493532-23493551 Msa1319730:CDS 45.0%
!! CGGTGTAAAAGTGTTGGTTG+TGG - chr8_4:23493538-23493557 Msa1319730:CDS 45.0%
GTTGGTTGTGGATGCTAGTT+CGG - chr8_4:23493550-23493569 Msa1319730:CDS 45.0%
TATGCTGCTGAACTACACAC+AGG - chr8_4:23493680-23493699 Msa1319730:CDS 45.0%
TGAACTACACACAGGTTTCG+TGG - chr8_4:23493688-23493707 Msa1319730:CDS 45.0%
TGAGGTTAACCTTGTGCTTG+TGG - chr8_4:23494178-23494197 Msa1319730:intron 45.0%
ACGACACTCCTTCCATATCA+TGG - chr8_4:23494399-23494418 Msa1319730:intron 45.0%
CGACACTCCTTCCATATCAT+GGG - chr8_4:23494400-23494419 Msa1319730:intron 45.0%
!!! CTCTTGGTTTTGGAGCCAAA+TGG - chr8_4:23494423-23494442 Msa1319730:intron 45.0%
TGCCAACCATCCATGAATAC+AGG - chr8_4:23494593-23494612 Msa1319730:intron 45.0%
CTCCTGTATTCATGGATGGT+TGG + chr8_4:23494598-23494617 None:intergenic 45.0%
CTCCTGCATTCAAAGATGGT+TGG + chr8_4:23494655-23494674 None:intergenic 45.0%
AAGACTCTCCGAAGTGCAAA+TGG - chr8_4:23494677-23494696 Msa1319730:CDS 45.0%
CAGTACTCCTGAACAGGAAA+AGG - chr8_4:23494732-23494751 Msa1319730:CDS 45.0%
GGACAACTTGAGCTTTCCAA+GGG + chr8_4:23495505-23495524 None:intergenic 45.0%
! CTTGGAAAGCTCAAGTTGTC+CGG - chr8_4:23495504-23495523 Msa1319730:intron 45.0%
! TTTTGCCTGAAAGGTGACTC+CGG + chr8_4:23495526-23495545 None:intergenic 45.0%
!! GACAGCTGATTTTGCCTGAA+AGG + chr8_4:23495535-23495554 None:intergenic 45.0%
AAATCAGCTGTCTCTGCCAT+TGG - chr8_4:23495543-23495562 Msa1319730:intron 45.0%
CATGTGCGAACCATTATGCA+TGG - chr8_4:23495726-23495745 Msa1319730:intron 45.0%
!!! TTTTCATCTGGTGGTAGTGG+CGG + chr8_4:23495763-23495782 None:intergenic 45.0%
!!! GCTTTTTCATCTGGTGGTAG+TGG + chr8_4:23495766-23495785 None:intergenic 45.0%
TATATCATTGTGCGTGCGCT+CGG - chr8_4:23495887-23495906 Msa1319730:CDS 45.0%
TTCTGACGAGTTCCAGTCAG+CGG - chr8_4:23493484-23493503 Msa1319730:CDS 50.0%
GGTTAACCTTGTGCTTGTGG+AGG - chr8_4:23494181-23494200 Msa1319730:intron 50.0%
ACTGAACCTCCACAAGCACA+AGG + chr8_4:23494190-23494209 None:intergenic 50.0%
ATCTCAGCGACAAGCATGTG+AGG + chr8_4:23494320-23494339 None:intergenic 50.0%
CACATGCTTGTCGCTGAGAT+CGG - chr8_4:23494320-23494339 Msa1319730:intron 50.0%
ACATGCTTGTCGCTGAGATC+GGG - chr8_4:23494321-23494340 Msa1319730:intron 50.0%
CCAAGAGCCCATGATATGGA+AGG + chr8_4:23494410-23494429 None:intergenic 50.0%
CCTTCCATATCATGGGCTCT+TGG - chr8_4:23494407-23494426 Msa1319730:intron 50.0%
AAGCAGTACCTGACTTCGTG+TGG - chr8_4:23494531-23494550 Msa1319730:intron 50.0%
GGCCAACCATCTTTGAATGC+AGG - chr8_4:23494650-23494669 Msa1319730:CDS 50.0%
! GAACCTACGTGCTACTGCAA+TGG - chr8_4:23495324-23495343 Msa1319730:intron 50.0%
GTTCCATTGCAGTAGCACGT+AGG + chr8_4:23495330-23495349 None:intergenic 50.0%
CGGACAACTTGAGCTTTCCA+AGG + chr8_4:23495506-23495525 None:intergenic 50.0%
!! GCAGGACTTTGCTGTTCCAA+TGG + chr8_4:23495562-23495581 None:intergenic 50.0%
AAACCTACCAAGGCTGGATG+TGG + chr8_4:23495590-23495609 None:intergenic 50.0%
TCGCACATGTCAAAGCTCAG+CGG + chr8_4:23495715-23495734 None:intergenic 50.0%
ATATCATTGTGCGTGCGCTC+GGG - chr8_4:23495888-23495907 Msa1319730:CDS 50.0%
AGGTACCATGGTCCAGTAGC+CGG - chr8_4:23493518-23493537 Msa1319730:CDS 55.0%
! TTACACCGGCTACTGGACCA+TGG + chr8_4:23493526-23493545 None:intergenic 55.0%
GGAGGTTCAGTTGCCAGACA+TGG - chr8_4:23494199-23494218 Msa1319730:intron 55.0%
ACCTTTGGCGATTCGCAGTG+TGG - chr8_4:23494483-23494502 Msa1319730:CDS 55.0%
TCCACACTGCGAATCGCCAA+AGG + chr8_4:23494487-23494506 None:intergenic 55.0%
GACAGATGCCACACGAAGTC+AGG + chr8_4:23494542-23494561 None:intergenic 55.0%
! TGGTTGGCATTTGGGACCCT+TGG + chr8_4:23494582-23494601 None:intergenic 55.0%
ATAGGCACACTTGGGGCAAC+TGG - chr8_4:23495107-23495126 Msa1319730:intron 55.0%
AGATCCTTCGGATCGGCATC+TGG + chr8_4:23495433-23495452 None:intergenic 55.0%
GTTGTCCGGAGTCACCTTTC+AGG - chr8_4:23495518-23495537 Msa1319730:intron 55.0%
ACACCACATCCAGCCTTGGT+AGG - chr8_4:23495584-23495603 Msa1319730:intron 55.0%
TGTAGACTCACTCGTGCTCG+CGG + chr8_4:23495914-23495933 None:intergenic 55.0%
!! GCGAAGCTGAGGAGGTACCA+TGG - chr8_4:23493506-23493525 Msa1319730:CDS 60.0%
CGATCCAGATGCCGATCCGA+AGG - chr8_4:23495426-23495445 Msa1319730:intron 60.0%
!! TCTGGTGGTAGTGGCGGCAT+AGG + chr8_4:23495757-23495776 None:intergenic 60.0%
TCCAGTCAGCGGCGAAGCTG+AGG - chr8_4:23493495-23493514 Msa1319730:CDS 65.0%
TCCTCAGCTTCGCCGCTGAC+TGG + chr8_4:23493499-23493518 None:intergenic 65.0%
AGTCAGCGGCGAAGCTGAGG+AGG - chr8_4:23493498-23493517 Msa1319730:CDS 65.0%
CTTGGGGCAACTGGTGCTCG+AGG - chr8_4:23495116-23495135 Msa1319730:intron 65.0%
AGGCTGGATGTGGTGTGCGC+AGG + chr8_4:23495580-23495599 None:intergenic 65.0%
GCGCACACCACATCCAGCCT+TGG - chr8_4:23495580-23495599 Msa1319730:intron 65.0%
GTGCTCGAGGCTCCCACCAG+TGG - chr8_4:23495129-23495148 Msa1319730:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 23493479 23495942 23493479 ID=Msa1319730;Name=Msa1319730
chr8_4 mRNA 23493479 23495942 23493479 ID=Msa1319730-mRNA-1;Parent=Msa1319730;Name=Msa1319730-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|5|0|481
chr8_4 exon 23495801 23495942 23495801 ID=Msa1319730-mRNA-1:exon:8169;Parent=Msa1319730-mRNA-1
chr8_4 exon 23495146 23495298 23495146 ID=Msa1319730-mRNA-1:exon:8168;Parent=Msa1319730-mRNA-1
chr8_4 exon 23494603 23495040 23494603 ID=Msa1319730-mRNA-1:exon:8167;Parent=Msa1319730-mRNA-1
chr8_4 exon 23494439 23494512 23494439 ID=Msa1319730-mRNA-1:exon:8166;Parent=Msa1319730-mRNA-1
chr8_4 exon 23493479 23494114 23493479 ID=Msa1319730-mRNA-1:exon:8165;Parent=Msa1319730-mRNA-1
chr8_4 CDS 23495801 23495942 23495801 ID=Msa1319730-mRNA-1:cds;Parent=Msa1319730-mRNA-1
chr8_4 CDS 23495146 23495298 23495146 ID=Msa1319730-mRNA-1:cds;Parent=Msa1319730-mRNA-1
chr8_4 CDS 23494603 23495040 23494603 ID=Msa1319730-mRNA-1:cds;Parent=Msa1319730-mRNA-1
chr8_4 CDS 23494439 23494512 23494439 ID=Msa1319730-mRNA-1:cds;Parent=Msa1319730-mRNA-1
chr8_4 CDS 23493479 23494114 23493479 ID=Msa1319730-mRNA-1:cds;Parent=Msa1319730-mRNA-1
Gene Sequence

>Msa1319730

ATGGATTCTGACGAGTTCCAGTCAGCGGCGAAGCTGAGGAGGTACCATGGTCCAGTAGCCGGTGTAAAAGTGTTGGTTGTGGATGCTAGTTCGGCATGCCGAACAATTGTATCAAAAATGCTTCTTTCACTTGGATATGAAGTTATGACGGCTTCACTAGCTTCCGATGCATTATCCATTATCTGTGAGAAGAAGAATGAGGTTAACCTTGTGCTTGTGGAGGTTCAGTTGCCAGACATGGAAATCTATGATCTCATAGGGAAAATGGGAGAAAGCTCTAATCTCCCGTCTTTCATTATGACTGCTTATGATGACGACACTCCTTCCATATCATGGGCTCTTGGTTTTGGAGCCAAATGGTGTTTTAGAAAGCCAGTTCAGATTTCTGATCTACATGACCTTTGGCGATTCGCAGTGTGGAACCGATATGAAACAACAGTCATTGAAGCAGTACCTGACTTCGTGTGGCATCTGTCAGATGTGATTATGGCAACCAAGGGTCCCAAATGCCAACCATCCATGAATACAGGAGAATGGTTTCGCCAAAATGCAATTGGTCTAGAAGGCCAACCATCTTTGAATGCAGGAGAAAAGACTCTCCGAAGTGCAAATGGTAAAGAACAGGAATTTCCTGATAAAGATAACTCAGTACTCCTGAACAGGAAAAGGAAAAGCTGGACTGATGATTTACATATGAAGTTCTTGGAAGCTGTTGAAATAGCAGGAATCGATGCTGGTTCGAAGACAATATTCCAGATTATGAATGTGGAAGGGTTTACCAAAGAGAGTGCCTCAAACTATTTACAGAGATATCGTCAATCTATGAACCTACGTGCTACTGCAATGGAACAGCATGTGAATGAATATGTGTCTCGGACTAACAAAAGTCAAGGAAAACTCCCTCTAGAAAAACAAGTTTTGATCAACGATCCAGATGCCGATCCGAAGGATCTCAGTAATTTTGTGCCTGTGCATATTCTTGACACTTCTGCTACTTTTCTTCCCTTGGAAAGCTCAAGTTGTCCGGAGTCACCTTTCAGGCAAAATCAGCTGTCTCTGCCATTGGAACAGCAAAGTCCTGCGCACACCACATCCAGCCTTGGTAGGTTTATAAACCGTGTTAATGATGATGTATCTGAAAAGATCAGCCAGGTTTTGAATACAGCAGATGGAGAAGTTTCTAATGCAGGACAACAGTTGAATTTTTCAACACCGCTGAGCTTTGACATGTGCGAACCATTATGCATGGTGCTTCCTATGCCGCCACTACCACCAGATGAAAAAGCATACTATGATAAGTTTTTTTATGCAAAAGAAACTCAGCTGTTTACTGATGAGGATTTGAAAATGTGGTTATCAACGATTCGAGGTATGAACATGCCAGTAATATATCATTGTGCGTGCGCTCGGGCCGCGAGCACGAGTGAGTCTACATTTTCTTCT
Protein sequence

>Msa1319730

MDSDEFQSAAKLRRYHGPVAGVKVLVVDASSACRTIVSKMLLSLGYEVMTASLASDALSIICEKKNEVNLVLVEVQLPDMEIYDLIGKMGESSNLPSFIMTAYDDDTPSISWALGFGAKWCFRKPVQISDLHDLWRFAVWNRYETTVIEAVPDFVWHLSDVIMATKGPKCQPSMNTGEWFRQNAIGLEGQPSLNAGEKTLRSANGKEQEFPDKDNSVLLNRKRKSWTDDLHMKFLEAVEIAGIDAGSKTIFQIMNVEGFTKESASNYLQRYRQSMNLRATAMEQHVNEYVSRTNKSQGKLPLEKQVLINDPDADPKDLSNFVPVHILDTSATFLPLESSSCPESPFRQNQLSLPLEQQSPAHTTSSLGRFINRVNDDVSEKISQVLNTADGEVSNAGQQLNFSTPLSFDMCEPLCMVLPMPPLPPDEKAYYDKFFYAKETQLFTDEDLKMWLSTIRGMNMPVIYHCACARAASTSESTFSS