Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1320760 | sp|Q9T020|Y4391_ARATH | 68.062 | 836 | 245 | 6 | 26 | 848 | 52 | 878 | 0.0 | 1178 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1320760 | MtrunA17_Chr8g0352381 | 97.642 | 848 | 20 | 0 | 1 | 848 | 34 | 881 | 0.0 | 1719 |
| Msa1320760 | MtrunA17_Chr4g0061081 | 53.935 | 775 | 333 | 6 | 25 | 776 | 31 | 804 | 0.0 | 835 |
| Msa1320760 | MtrunA17_Chr2g0318301 | 55.701 | 763 | 316 | 12 | 26 | 775 | 27 | 780 | 0.0 | 815 |
| Msa1320760 | MtrunA17_Chr4g0030441 | 49.940 | 833 | 384 | 14 | 23 | 845 | 25 | 834 | 0.0 | 772 |
| Msa1320760 | MtrunA17_Chr2g0327671 | 50.127 | 786 | 360 | 14 | 17 | 784 | 19 | 790 | 0.0 | 744 |
| Msa1320760 | MtrunA17_Chr1g0202711 | 46.200 | 842 | 390 | 24 | 23 | 848 | 38 | 832 | 0.0 | 677 |
| Msa1320760 | MtrunA17_Chr2g0292501 | 44.994 | 809 | 393 | 17 | 4 | 777 | 10 | 801 | 0.0 | 656 |
| Msa1320760 | MtrunA17_Chr6g0468691 | 47.631 | 781 | 360 | 24 | 22 | 776 | 19 | 776 | 0.0 | 645 |
| Msa1320760 | MtrunA17_Chr1g0190581 | 47.522 | 787 | 361 | 20 | 30 | 775 | 27 | 802 | 0.0 | 644 |
| Msa1320760 | MtrunA17_Chr7g0274261 | 45.409 | 806 | 390 | 21 | 8 | 775 | 14 | 807 | 0.0 | 615 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1320760 | AT4G39110.1 | 68.062 | 836 | 245 | 6 | 26 | 848 | 52 | 878 | 0.0 | 1178 |
| Msa1320760 | AT2G21480.1 | 65.827 | 834 | 271 | 5 | 21 | 848 | 46 | 871 | 0.0 | 1145 |
| Msa1320760 | AT5G61350.1 | 58.949 | 799 | 289 | 13 | 22 | 785 | 24 | 818 | 0.0 | 938 |
| Msa1320760 | AT5G54380.1 | 52.676 | 822 | 353 | 13 | 6 | 805 | 7 | 814 | 0.0 | 798 |
| Msa1320760 | AT5G59700.1 | 52.026 | 765 | 341 | 18 | 26 | 784 | 25 | 769 | 0.0 | 732 |
| Msa1320760 | AT3G46290.1 | 51.436 | 766 | 347 | 16 | 26 | 784 | 25 | 772 | 0.0 | 731 |
| Msa1320760 | AT5G24010.1 | 46.092 | 870 | 395 | 24 | 3 | 848 | 5 | 824 | 0.0 | 669 |
| Msa1320760 | AT1G30570.1 | 44.621 | 818 | 415 | 15 | 31 | 819 | 32 | 840 | 0.0 | 664 |
| Msa1320760 | AT3G51550.1 | 42.369 | 878 | 447 | 22 | 22 | 848 | 26 | 895 | 0.0 | 624 |
| Msa1320760 | AT5G28680.1 | 44.571 | 792 | 389 | 21 | 23 | 776 | 21 | 800 | 0.0 | 612 |
Find 180 sgRNAs with CRISPR-Local
Find 214 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTTGAGGATGAGAATG+AGG | 0.524684 | 8_4:-25554225 | Msa1320760:CDS |
| AAAAGTTGCAATTTCTAATT+TGG | 0.122086 | 8_4:-25555584 | Msa1320760:CDS |
| AAAGACGCGCCTATGGGACA+AGG | 0.623518 | 8_4:-25554632 | Msa1320760:CDS |
| AACAAGTTAACTTAGCCGAT+TGG | 0.493959 | 8_4:-25554451 | Msa1320760:CDS |
| AACACAGTGAATAGTTTGGA+TGG | 0.610652 | 8_4:-25555397 | Msa1320760:CDS |
| AACCTTTGTTTCCAAGATAG+TGG | 0.395472 | 8_4:+25554797 | None:intergenic |
| AACGCAGCCGAATTCTTCAA+AGG | 0.621658 | 8_4:+25556075 | None:intergenic |
| AAGATAAGAGAAGAATGACC+AGG | 0.592806 | 8_4:+25556553 | None:intergenic |
| AAGCAACACAGTGAATAGTT+TGG | 0.427000 | 8_4:-25555401 | Msa1320760:CDS |
| AAGTCTGATGTTTACTCGTT+TGG | 0.347506 | 8_4:-25554533 | Msa1320760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGCTAAGTATTCATTCCATT+TGG | - | chr8_4:25554306-25554325 | Msa1320760:CDS | 30.0% | |
| TTGGATTTATCAGCTATAAC+TGG | - | chr8_4:25555039-25555058 | Msa1320760:CDS | 30.0% | |
| TGCAATTATGAATGGTATTG+AGG | - | chr8_4:25555170-25555189 | Msa1320760:CDS | 30.0% | |
| ATGAGTAGCAAGAATTCAAT+AGG | - | chr8_4:25555429-25555448 | Msa1320760:CDS | 30.0% | |
| TGAGTAGCAAGAATTCAATA+GGG | - | chr8_4:25555430-25555449 | Msa1320760:CDS | 30.0% | |
| TCCAAAAACATTATTGGTGT+TGG | - | chr8_4:25555537-25555556 | Msa1320760:CDS | 30.0% | |
| TATTTAGGTGTGATTGATGA+AGG | - | chr8_4:25555576-25555595 | Msa1320760:CDS | 30.0% | |
| ATTTAGGTGTGATTGATGAA+GGG | - | chr8_4:25555577-25555596 | Msa1320760:CDS | 30.0% | |
| TAAGCTTAGACATAGACATT+TGG | - | chr8_4:25555680-25555699 | Msa1320760:CDS | 30.0% | |
| GCAAATTTCTTCATAGATTC+GGG | + | chr8_4:25556248-25556267 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 25554040 | 25556586 | 25554040 | ID=Msa1320760;Name=Msa1320760 |
| chr8_4 | mRNA | 25554040 | 25556586 | 25554040 | ID=Msa1320760-mRNA-1;Parent=Msa1320760;Name=Msa1320760-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|848 |
| chr8_4 | exon | 25554040 | 25556586 | 25554040 | ID=Msa1320760-mRNA-1:exon:8756;Parent=Msa1320760-mRNA-1 |
| chr8_4 | CDS | 25554040 | 25556586 | 25554040 | ID=Msa1320760-mRNA-1:cds;Parent=Msa1320760-mRNA-1 |
| Gene Sequence |
>Msa1320760 ATGGCTATCCTCCTGGTCATTCTTCTCTTATCTTTTTCTTCACCTTCATTAGCTCTTCCTTCTTCGTCTCCAAGTTTCGTTCCGAAAGACAGTTTTCTTATTGATTGTGGTTCTGAAAATGTAGTTACTTTACCTGATGGAAGACAATTCAAATCAGATCCACAAGCAAATTCATTTTTACAAGCTAAGGATGATTACAAAGTTTCAGCAGATGATGCTAATGTTCCTTCACCTATATATAAAAATGCTAGAATCTTCATTCAAGAAGCTAAGTATTCATTCCATTTGGTTCAGCCTGGTTTTCATTGGATTCGCCTTCATTTCTATCCGATCAAAAACAGTGTTTTCGATCTTCAAAAGGCGACGTTTTCTGTCAACACAAATACTTTTGTTCTTCTTCATAGCTTCAATGTGAATAACACTGACAAAGCAATCCTCAAAGAGTATCTCATCAATGCAACAGAACCACAGTTGATGTTGTCCTTCATTCCTTTGAAGAATTCGGCTGCGTTTATCAATGCAATCGAGGTTGTTTCGGCGCCAGATAACCTAATCTTTGACACCGGCTCGGGACTTTTCCCAGTCGCCCCGGTTAACGGATTAACAGCCTATGCTTTCCAGCCTGTTTATAGGTTGAACAATGGAGGACCTTTAATTACATCAGCAAATGATACACTTGGAAGAACTTGGGAATCTGATGAACCTTATCTTACAAATAAGAATCTTGGAAAGAGTGTTACTGTTGCTACTAATGTTGTTAAGTTTCCTCAAGACCGTCCGGATATTTCGCCTTTGATTGCACCACAAACTGTTTATTCTTCTGCCACTGAGATGGGTAATGCTGATGTGAATCTACCAAATTTCAATGTATCATGGAAATTTGATGTTGACACTTCTTTTAGTTACCTTGTTAGGTTGCATTTTTGTGACATTGTGAGTAAAGGACTTAATCAACTTTACTTCAATGTTTATGTTAATGGAAAAGTTGCAATTTCTAATTTGGATTTATCAGCTATAACTGGTGCATTGGCAACACCATATTATAAAGATTTTGTTGTGAATGCAACATTGATGACTCAAGGATTGAGTGTTCAAGTTGGTCCTGCAGATGCTAATGGTGGAAATGGTAATGCAATTATGAATGGTATTGAGGTTTTGAAGATAAGCAACACAGTGAATAGTTTGGATGGTGAGTTTGGTGTTGATGGAAGGAGAGTTGGTGGTTCGAACCGTGGAACGGTTGCAGCGGTCGGTTTTGCTATGATGTTTGGAGCATTTGTTGGTCTTGGTGCTATGGTGATCAAGTGGCATAAGAGGCCTCAAGATTGGCAAAAGAGGAATAGTTTCTCTTCATGGTTGCTTCCTTTACACGCCGGTGACACAAGTTTCATGAGTAGCAAGAATTCAATAGGGAAAAGCAACATTTTCTCATCATCAATGGGATTAGGAAGGATTTTCTCCTTTTCAGAAATACAAGAAGCAACAAAGAATTTTGACTCCAAAAACATTATTGGTGTTGGTGGTTTTGGTAATGTTTATTTAGGTGTGATTGATGAAGGGGTGCAAGTTGCTGTCAAGAGAGGAAATCCACAATCAGAACAAGGTATCAATGAATTCCAGACTGAAATCCAAATGTTGTCTAAGCTTAGACATAGACATTTGGTTTCAATGATTGGATATTGTGATGAAAATGAAGAAATGATTCTTGTTTATGAGTACATGCCTAATGGACATCTTAGAGACCATCTTTATGGAAAGAACATGCCACCACTATCTTGGAAACAAAGGTTGGAGATTTGTATTGGATCGGCAAGAGGTCTTCATTACCTTCACACGGGTACGGCACAAGGAATCATTCATCGCGACGTTAAGACAACAAACATCTTGCTTGATGAGAATTTCACGGCTAAGGTATCCGATTTTGGACTTTCAAAAGACGCGCCTATGGGACAAGGTCACGTTAGTACTGCGGTGAAAGGTAGTTTTGGTTATCTTGACCCTGAGTACTTCAGGAGACAACAATTGACCGAAAAGTCTGATGTTTACTCGTTTGGAGTAGTCCTGCTCGAGGCATTGTGCGCCAGGCCGGCTATTAACCCTCAGTTACCTAGAGAACAAGTTAACTTAGCCGATTGGGCTATGCAATGGAAGAGAAAGGGTTTGCTTGATAAAATCATTGACCCTCTTCTTGTCGGTTCGATTAATCCCGAATCTATGAAGAAATTTGCTGAGGCTGCTGAGAAATGTTTGGCTGATCATGGTGTTGATAGGCCTTCAATGGGAGATGTTCTTTGGAATTTGGAGTATGCATTGCAGCTTCAAGAAGCTTTTACACAAGGAAAAGTTGAGGATGAGAATGAGGCTAAATCTGCTGCTGTTGTTGTTCCTACTTCACCTGCTCCTCCTACTACACCCCCCTCTGTTACTGATTGTCCCCCCACAACTCCTCAACAAGAACTAAATAATAGTCCTGCTGAAGTTCAAGCTATTGATGGCCATTCTGGAACTGCAATGTTTGCACAGTTTAGTAATCTTAATGGAAGGTGA |
| Protein sequence |
>Msa1320760 MAILLVILLLSFSSPSLALPSSSPSFVPKDSFLIDCGSENVVTLPDGRQFKSDPQANSFLQAKDDYKVSADDANVPSPIYKNARIFIQEAKYSFHLVQPGFHWIRLHFYPIKNSVFDLQKATFSVNTNTFVLLHSFNVNNTDKAILKEYLINATEPQLMLSFIPLKNSAAFINAIEVVSAPDNLIFDTGSGLFPVAPVNGLTAYAFQPVYRLNNGGPLITSANDTLGRTWESDEPYLTNKNLGKSVTVATNVVKFPQDRPDISPLIAPQTVYSSATEMGNADVNLPNFNVSWKFDVDTSFSYLVRLHFCDIVSKGLNQLYFNVYVNGKVAISNLDLSAITGALATPYYKDFVVNATLMTQGLSVQVGPADANGGNGNAIMNGIEVLKISNTVNSLDGEFGVDGRRVGGSNRGTVAAVGFAMMFGAFVGLGAMVIKWHKRPQDWQKRNSFSSWLLPLHAGDTSFMSSKNSIGKSNIFSSSMGLGRIFSFSEIQEATKNFDSKNIIGVGGFGNVYLGVIDEGVQVAVKRGNPQSEQGINEFQTEIQMLSKLRHRHLVSMIGYCDENEEMILVYEYMPNGHLRDHLYGKNMPPLSWKQRLEICIGSARGLHYLHTGTAQGIIHRDVKTTNILLDENFTAKVSDFGLSKDAPMGQGHVSTAVKGSFGYLDPEYFRRQQLTEKSDVYSFGVVLLEALCARPAINPQLPREQVNLADWAMQWKRKGLLDKIIDPLLVGSINPESMKKFAEAAEKCLADHGVDRPSMGDVLWNLEYALQLQEAFTQGKVEDENEAKSAAVVVPTSPAPPTTPPSVTDCPPTTPQQELNNSPAEVQAIDGHSGTAMFAQFSNLNGR* |
