AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1321270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1321270 MtrunA17_Chr1g0172971 77.273 132 15 1 1 132 1 117 2.08e-59 207
Msa1321270 MtrunA17_Chr3g0088371 87.234 94 12 0 527 620 21 114 7.42e-48 166
Msa1321270 MtrunA17_Chr3g0082121 23.454 469 247 12 57 431 49 499 2.67e-20 95.5
Msa1321270 MtrunA17_Chr7g0258861 27.239 268 144 6 46 281 2 250 5.13e-20 91.3
Msa1321270 MtrunA17_Chr2g0324011 25.256 293 154 8 281 554 325 571 2.11e-19 92.4
Msa1321270 MtrunA17_Chr6g0475591 84.444 45 7 0 204 248 1 45 7.04e-19 81.3
Msa1321270 MtrunA17_Chr1g0202341 22.056 603 326 23 50 551 50 609 4.70e-17 85.1
Msa1321270 MtrunA17_Chr8g0363841 26.132 287 158 9 282 554 334 580 1.18e-13 74.3
Msa1321270 MtrunA17_Chr1g0164591 27.742 155 92 3 147 281 1 155 1.46e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 72 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATCATGATCTTCAAAACT+TGG 0.305304 8_4:-26650234 None:intergenic
TTACTTCTCGAAATGATCTA+AGG 0.311482 8_4:-26648357 None:intergenic
TCTGATGTTGTCCTGTTGTT+AGG 0.316425 8_4:-26648166 None:intergenic
CAACTAGATTATTCTCTATT+CGG 0.323831 8_4:+26648673 Msa1321270:CDS
GCCGATATTTATTGTTTGTT+CGG 0.341758 8_4:-26648723 None:intergenic
GCATTTATTCACAGGCAGTA+TGG 0.343353 8_4:+26650042 Msa1321270:CDS
GAGGGATAAGCATGCTGAAT+TGG 0.381188 8_4:+26649927 Msa1321270:CDS
TTGATATCGTTGTGCTTGAT+TGG 0.382195 8_4:-26650502 None:intergenic
AACAATAAATATCGGCCATT+AGG 0.388497 8_4:+26648730 Msa1321270:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 8_4:+26648722 Msa1321270:CDS
TTCCTTCAAATACTATTATG+AGG 0.392551 8_4:+26650068 Msa1321270:CDS
GAGAACGTCTTATGAAGTTA+TGG 0.394356 8_4:+26648432 Msa1321270:CDS
GCATGCTGAATTGGACTTCA+AGG 0.414790 8_4:+26649936 Msa1321270:CDS
GAAAGGTTTGGTTGTAAAGC+TGG 0.427668 8_4:+26648235 Msa1321270:CDS
CTAGACGGTGTTCTTGAAGC+TGG 0.427944 8_4:+26650594 Msa1321270:CDS
GAAGTTATGGGTCAAGGAGT+TGG 0.428685 8_4:+26648445 Msa1321270:CDS
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 8_4:+26648370 Msa1321270:CDS
GAAACTTCATCACTGATTCA+TGG 0.432651 8_4:+26647866 Msa1321270:CDS
GAGGAGTTGCTTCTTTCTAA+AGG 0.437852 8_4:+26650627 Msa1321270:CDS
TCCATGATTCTTTATTGCTA+GGG 0.444266 8_4:-26647955 None:intergenic
TTCCATGATTCTTTATTGCT+AGG 0.445442 8_4:-26647956 None:intergenic
ATGTGTATAAGATATATAGA+TGG 0.452819 8_4:+26648287 Msa1321270:CDS
GACCGCATCAGCTGACTGTT+AGG 0.462687 8_4:-26649988 None:intergenic
GCTCTTATAAATTCTAAAGC+TGG 0.462891 8_4:+26648400 Msa1321270:CDS
AGAACGTCTTATGAAGTTAT+GGG 0.466325 8_4:+26648433 Msa1321270:CDS
GTTTGCTTAATCCTAACAAC+AGG 0.467124 8_4:+26648155 Msa1321270:CDS
CGCTTTAAAACATATTGTCC+AGG 0.471916 8_4:-26650411 None:intergenic
ACAAAATAAGAAATCTGATA+AGG 0.474392 8_4:+26648084 Msa1321270:CDS
ATCATGATCGATTTGATGAA+AGG 0.476999 8_4:+26650247 Msa1321270:CDS
ACATTATAGTTCAGAGAATG+AGG 0.479730 8_4:+26649908 Msa1321270:CDS
CTTGCTTTGCATTTATTCAC+AGG 0.480587 8_4:+26650034 Msa1321270:CDS
TTTAAGTCTGAAAACCGAAA+AGG 0.483010 8_4:-26648484 None:intergenic
CTTACGAAAATCGTATGCAT+TGG 0.484399 8_4:+26649755 Msa1321270:CDS
CTCCTCATAATAGTATTTGA+AGG 0.485089 8_4:-26650070 None:intergenic
CCCCTAGCAATAAAGAATCA+TGG 0.497001 8_4:+26647954 Msa1321270:CDS
TATGAGGAGGACACGACAAA+AGG 0.501557 8_4:+26650084 Msa1321270:CDS
TAGACGGTGTTCTTGAAGCT+GGG 0.505924 8_4:+26650595 Msa1321270:CDS
AGCTGGAATATATTTGAAGC+TGG 0.507410 8_4:+26648252 Msa1321270:CDS
CTAAGGTAACACGAGTGCTC+TGG 0.508788 8_4:-26648340 None:intergenic
TAACAAACAAGGTTTCAAGA+AGG 0.511682 8_4:+26648129 Msa1321270:CDS
TAAAGAATCGAACAAGTGAA+TGG 0.519618 8_4:-26649876 None:intergenic
TTACAACCAAACCTTTCCTC+CGG 0.520136 8_4:-26648229 None:intergenic
CATAATAGTATTTGAAGGAA+AGG 0.525625 8_4:-26650065 None:intergenic
TGCATCTAAAACATCAACAT+TGG 0.528166 8_4:-26650190 None:intergenic
ATGAGGAGGACACGACAAAA+GGG 0.528774 8_4:+26650085 Msa1321270:CDS
TTCACAAACTCAATAACCAA+TGG 0.533066 8_4:-26648193 None:intergenic
CCATGATTCTTTATTGCTAG+GGG 0.537011 8_4:-26647954 None:intergenic
AATCGAACAAGTGAATGGTC+TGG 0.539718 8_4:-26649871 None:intergenic
AACAAACAAGGTTTCAAGAA+GGG 0.554561 8_4:+26648130 Msa1321270:CDS
GAGTGCTCTGGTTTATGTAA+TGG 0.565687 8_4:-26648328 None:intergenic
TAAGATATATAGATGGGATG+TGG 0.566406 8_4:+26648294 Msa1321270:CDS
GATCATGATCTTCAAAACTT+GGG 0.567815 8_4:-26650233 None:intergenic
CACGACAAAAGGGTCTGCGT+TGG 0.568588 8_4:+26650095 Msa1321270:CDS
GTCTTCAGTGCCAACATGGA+TGG 0.576478 8_4:-26650478 None:intergenic
GTTATGGGTCAAGGAGTTGG+AGG 0.578191 8_4:+26648448 Msa1321270:CDS
TCTTATGAAGTTATGGGTCA+AGG 0.580120 8_4:+26648439 Msa1321270:CDS
TCACTGATTCATGGTGAGAG+TGG 0.583529 8_4:+26647875 Msa1321270:CDS
TGAATAAATGCAAAGCAAGC+TGG 0.591761 8_4:-26650030 None:intergenic
ATCCTAACAGTCAGCTGATG+CGG 0.594920 8_4:+26649986 Msa1321270:CDS
TGTGTATAAGATATATAGAT+GGG 0.601002 8_4:+26648288 Msa1321270:CDS
GGGTCAAGGAGTTGGAGGCA+TGG 0.604978 8_4:+26648453 Msa1321270:CDS
CTTCAAATACTATTATGAGG+AGG 0.607664 8_4:+26650071 Msa1321270:CDS
TATGATGATGCTGATAATGA+CGG 0.609333 8_4:+26650162 Msa1321270:CDS
ATATCAACTTCATCTGAGAG+TGG 0.614980 8_4:+26647830 Msa1321270:CDS
AGCCACCTAAAATCTGAAGT+AGG 0.617789 8_4:-26649724 None:intergenic
GACAACATCAGACAATCCAT+TGG 0.621343 8_4:+26648177 Msa1321270:CDS
ATGATGATGCTGATAATGAC+GGG 0.625677 8_4:+26650163 Msa1321270:CDS
CTAAAATCGACCATCCATGT+TGG 0.637846 8_4:+26650468 Msa1321270:CDS
TGGTGTCTTCAGTGCCAACA+TGG 0.646482 8_4:-26650482 None:intergenic
TGTTATGTTTGTAACAAACA+AGG 0.647419 8_4:+26648118 Msa1321270:CDS
CATTATAGTTCAGAGAATGA+GGG 0.650774 8_4:+26649909 Msa1321270:CDS
TGGTGTGTACTTGTTTACAA+CGG 0.684217 8_4:-26650010 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTGTAAATTTTTTTTTC+TGG + chr8_4:26648819-26648838 Msa1321270:intron 15.0%
!!! ATGATGAAAAATTGTTTTAA+TGG + chr8_4:26649697-26649716 Msa1321270:CDS 15.0%
!!! AAAGAGATTGAAGATTTTTA+TGG + chr8_4:26648001-26648020 Msa1321270:CDS 20.0%
!!! ATAACACTGAATTTTCTATT+AGG + chr8_4:26648035-26648054 Msa1321270:CDS 20.0%
!! ACAAAATAAGAAATCTGATA+AGG + chr8_4:26648084-26648103 Msa1321270:CDS 20.0%
!! ATGTGTATAAGATATATAGA+TGG + chr8_4:26648287-26648306 Msa1321270:CDS 20.0%
!! TGTGTATAAGATATATAGAT+GGG + chr8_4:26648288-26648307 Msa1321270:CDS 20.0%
!!! TTTTGATGAGTTAACTAATT+TGG + chr8_4:26649627-26649646 Msa1321270:CDS 20.0%
!!! AATCAGATTTTGACTATAAT+TGG + chr8_4:26649668-26649687 Msa1321270:CDS 20.0%
!!! ATCAGATTTTGACTATAATT+GGG + chr8_4:26649669-26649688 Msa1321270:CDS 20.0%
!!! TGAAAAATTGTTTTAATGGA+AGG + chr8_4:26649701-26649720 Msa1321270:CDS 20.0%
! TGTTATGTTTGTAACAAACA+AGG + chr8_4:26648118-26648137 Msa1321270:CDS 25.0%
! TGGATATCATAGTAAAAAGA+TGG - chr8_4:26648601-26648620 None:intergenic 25.0%
!!! AAGACATAGCTAGTATTTTT+TGG + chr8_4:26648635-26648654 Msa1321270:CDS 25.0%
!!! AGACATAGCTAGTATTTTTT+GGG + chr8_4:26648636-26648655 Msa1321270:CDS 25.0%
! CAACTAGATTATTCTCTATT+CGG + chr8_4:26648673-26648692 Msa1321270:CDS 25.0%
! ACCGAACAAACAATAAATAT+CGG + chr8_4:26648722-26648741 Msa1321270:CDS 25.0%
!! TTACTTTCATTAAGTTGTTC+TGG + chr8_4:26648795-26648814 Msa1321270:intron 25.0%
! TAGTTCTTACTTGTTTGTTT+AGG + chr8_4:26648913-26648932 Msa1321270:intron 25.0%
! AGTTCTTACTTGTTTGTTTA+GGG + chr8_4:26648914-26648933 Msa1321270:intron 25.0%
! CAAACTTAAATGATGTTGAT+TGG + chr8_4:26649013-26649032 Msa1321270:intron 25.0%
!!! ATTTATATTTTGAACTGCCT+AGG + chr8_4:26649037-26649056 Msa1321270:intron 25.0%
! GAATACTTACTAGAATACTT+AGG + chr8_4:26649122-26649141 Msa1321270:intron 25.0%
!! TGTTGTATTTTGTTCTGATA+TGG + chr8_4:26649318-26649337 Msa1321270:intron 25.0%
! ATGATTGTCAAAACCAATAA+AGG - chr8_4:26649363-26649382 None:intergenic 25.0%
! CATAATAGTATTTGAAGGAA+AGG - chr8_4:26650068-26650087 None:intergenic 25.0%
! TTCCTTCAAATACTATTATG+AGG + chr8_4:26650068-26650087 Msa1321270:CDS 25.0%
!! TCAAAGTTTTCACAATTGAA+AGG + chr8_4:26650127-26650146 Msa1321270:CDS 25.0%
!! CAAAGTTTTCACAATTGAAA+GGG + chr8_4:26650128-26650147 Msa1321270:CDS 25.0%
TCCATGATTCTTTATTGCTA+GGG - chr8_4:26647958-26647977 None:intergenic 30.0%
TTCCATGATTCTTTATTGCT+AGG - chr8_4:26647959-26647978 None:intergenic 30.0%
TAACAAACAAGGTTTCAAGA+AGG + chr8_4:26648129-26648148 Msa1321270:CDS 30.0%
AACAAACAAGGTTTCAAGAA+GGG + chr8_4:26648130-26648149 Msa1321270:CDS 30.0%
TTCACAAACTCAATAACCAA+TGG - chr8_4:26648196-26648215 None:intergenic 30.0%
TGTGAAAGTGAAAGAAAAAC+CGG + chr8_4:26648210-26648229 Msa1321270:CDS 30.0%
TAAGATATATAGATGGGATG+TGG + chr8_4:26648294-26648313 Msa1321270:CDS 30.0%
TTACTTCTCGAAATGATCTA+AGG - chr8_4:26648360-26648379 None:intergenic 30.0%
!! GCTCTTATAAATTCTAAAGC+TGG + chr8_4:26648400-26648419 Msa1321270:CDS 30.0%
AGAACGTCTTATGAAGTTAT+GGG + chr8_4:26648433-26648452 Msa1321270:CDS 30.0%
TTTAAGTCTGAAAACCGAAA+AGG - chr8_4:26648487-26648506 None:intergenic 30.0%
GCCGATATTTATTGTTTGTT+CGG - chr8_4:26648726-26648745 None:intergenic 30.0%
AACAATAAATATCGGCCATT+AGG + chr8_4:26648730-26648749 Msa1321270:CDS 30.0%
GAAAACAAACACTAACCTAA+TGG - chr8_4:26648748-26648767 None:intergenic 30.0%
!!! GCATGTTGTTTTTTTTGTTC+TGG + chr8_4:26648841-26648860 Msa1321270:intron 30.0%
TAAGAACTATAGTCAATCCT+AGG - chr8_4:26648902-26648921 None:intergenic 30.0%
TTGTCTAAGTAAAAATGCCT+AGG - chr8_4:26649057-26649076 None:intergenic 30.0%
AGACTAGCAATGACTAATTA+AGG + chr8_4:26649205-26649224 Msa1321270:intron 30.0%
GACTAGCAATGACTAATTAA+GGG + chr8_4:26649206-26649225 Msa1321270:intron 30.0%
TAAATTTCAGCTGCCTTTAT+TGG + chr8_4:26649347-26649366 Msa1321270:intron 30.0%
! CATCGTAAGAGTCTTTTATT+TGG + chr8_4:26649380-26649399 Msa1321270:intron 30.0%
AATCGTTTGTGGTTTCTTAT+TGG - chr8_4:26649477-26649496 None:intergenic 30.0%
TGGTCTATATAAATCGTTTG+TGG - chr8_4:26649488-26649507 None:intergenic 30.0%
AAAACACCTGGAAAACTATT+CGG - chr8_4:26649536-26649555 None:intergenic 30.0%
GAGAATGCAAAGAAAAATCT+TGG + chr8_4:26649583-26649602 Msa1321270:intron 30.0%
TAAAGAATCGAACAAGTGAA+TGG - chr8_4:26649879-26649898 None:intergenic 30.0%
ACATTATAGTTCAGAGAATG+AGG + chr8_4:26649908-26649927 Msa1321270:CDS 30.0%
CATTATAGTTCAGAGAATGA+GGG + chr8_4:26649909-26649928 Msa1321270:CDS 30.0%
CTCCTCATAATAGTATTTGA+AGG - chr8_4:26650073-26650092 None:intergenic 30.0%
CTTCAAATACTATTATGAGG+AGG + chr8_4:26650071-26650090 Msa1321270:CDS 30.0%
! TATGATGATGCTGATAATGA+CGG + chr8_4:26650162-26650181 Msa1321270:CDS 30.0%
! TGCATCTAAAACATCAACAT+TGG - chr8_4:26650193-26650212 None:intergenic 30.0%
GATCATGATCTTCAAAACTT+GGG - chr8_4:26650236-26650255 None:intergenic 30.0%
!! ATCATGATCGATTTGATGAA+AGG + chr8_4:26650247-26650266 Msa1321270:CDS 30.0%
!!! TTGACATGTTTTGAAGATCT+TGG + chr8_4:26650344-26650363 Msa1321270:intron 30.0%
!!! CATTTTTTGAAGACAATACC+TGG + chr8_4:26650393-26650412 Msa1321270:CDS 30.0%
!!! GACAATATGTTTTAAAGCGT+TGG + chr8_4:26650415-26650434 Msa1321270:CDS 30.0%
ATATCAACTTCATCTGAGAG+TGG + chr8_4:26647830-26647849 Msa1321270:CDS 35.0%
!! GAAACTTCATCACTGATTCA+TGG + chr8_4:26647866-26647885 Msa1321270:CDS 35.0%
CCATGATTCTTTATTGCTAG+GGG - chr8_4:26647957-26647976 None:intergenic 35.0%
GTTTGCTTAATCCTAACAAC+AGG + chr8_4:26648155-26648174 Msa1321270:CDS 35.0%
! AGCTGGAATATATTTGAAGC+TGG + chr8_4:26648252-26648271 Msa1321270:CDS 35.0%
CGAGAAGTAAATGAAGTTCA+AGG + chr8_4:26648370-26648389 Msa1321270:CDS 35.0%
GAGAACGTCTTATGAAGTTA+TGG + chr8_4:26648432-26648451 Msa1321270:CDS 35.0%
TCTTATGAAGTTATGGGTCA+AGG + chr8_4:26648439-26648458 Msa1321270:CDS 35.0%
GACAATTCGTCAAAAAGAGA+TGG + chr8_4:26648519-26648538 Msa1321270:CDS 35.0%
AATTCGTCAAAAAGAGATGG+TGG + chr8_4:26648522-26648541 Msa1321270:CDS 35.0%
!!! GCTAGTATTTTTTGGGCAAA+TGG + chr8_4:26648643-26648662 Msa1321270:CDS 35.0%
CTGGCATGTGTATTATAAGT+AGG + chr8_4:26648860-26648879 Msa1321270:intron 35.0%
AAAACCCCATGAAACCAAAT+GGG - chr8_4:26648954-26648973 None:intergenic 35.0%
AAAAACCCCATGAAACCAAA+TGG - chr8_4:26648955-26648974 None:intergenic 35.0%
!! CATCTTTCAGGTTTGATATC+TGG - chr8_4:26648985-26649004 None:intergenic 35.0%
CAGATATCAAACCTGAAAGA+TGG + chr8_4:26648983-26649002 Msa1321270:intron 35.0%
TTTGTCTCAAACCATCTTTC+AGG - chr8_4:26648997-26649016 None:intergenic 35.0%
! ATTGAGTTTACGCATTGATG+AGG + chr8_4:26649156-26649175 Msa1321270:intron 35.0%
AGCAATGACTAATTAAGGGT+TGG + chr8_4:26649210-26649229 Msa1321270:intron 35.0%
TGACTAATTAAGGGTTGGAT+TGG + chr8_4:26649215-26649234 Msa1321270:intron 35.0%
!!! AGACAACAGCTACTTTTGAT+TGG + chr8_4:26649423-26649442 Msa1321270:intron 35.0%
AACACAATAACGCATTAGAC+TGG - chr8_4:26649508-26649527 None:intergenic 35.0%
! TGAATTCCGAATAGTTTTCC+AGG + chr8_4:26649527-26649546 Msa1321270:intron 35.0%
! AGTTTTCCAGGTGTTTTTCA+TGG + chr8_4:26649539-26649558 Msa1321270:intron 35.0%
!!! GTTTTTCATGGCTTTGTTCA+TGG + chr8_4:26649551-26649570 Msa1321270:intron 35.0%
! GCATACGATTTTCGTAAGTT+TGG - chr8_4:26649753-26649772 None:intergenic 35.0%
CTTACGAAAATCGTATGCAT+TGG + chr8_4:26649755-26649774 Msa1321270:CDS 35.0%
!!! TATGCATTGGTTTTCAGCTT+GGG + chr8_4:26649768-26649787 Msa1321270:CDS 35.0%
! TCTTTCAATGCTTTTCTTCG+TGG + chr8_4:26649838-26649857 Msa1321270:CDS 35.0%
TGGTGTGTACTTGTTTACAA+CGG - chr8_4:26650013-26650032 None:intergenic 35.0%
TGAATAAATGCAAAGCAAGC+TGG - chr8_4:26650033-26650052 None:intergenic 35.0%
CTTGCTTTGCATTTATTCAC+AGG + chr8_4:26650034-26650053 Msa1321270:CDS 35.0%
! ATGATGATGCTGATAATGAC+GGG + chr8_4:26650163-26650182 Msa1321270:CDS 35.0%
CGATCATGATCTTCAAAACT+TGG - chr8_4:26650237-26650256 None:intergenic 35.0%
ATGTCGTATGTTTGAGAACA+GGG + chr8_4:26650311-26650330 Msa1321270:intron 35.0%
!! CGCTTTAAAACATATTGTCC+AGG - chr8_4:26650414-26650433 None:intergenic 35.0%
!!! GATTTTAGCTTATCGACAGA+CGG - chr8_4:26650456-26650475 None:intergenic 35.0%
TTGATATCGTTGTGCTTGAT+TGG - chr8_4:26650505-26650524 None:intergenic 35.0%
!! GCATCTGAAGTTTTTCTAGA+CGG + chr8_4:26650579-26650598 Msa1321270:CDS 35.0%
CCCCTAGCAATAAAGAATCA+TGG + chr8_4:26647954-26647973 Msa1321270:CDS 40.0%
TCTGATGTTGTCCTGTTGTT+AGG - chr8_4:26648169-26648188 None:intergenic 40.0%
GACAACATCAGACAATCCAT+TGG + chr8_4:26648177-26648196 Msa1321270:CDS 40.0%
GAAAGTGAAAGAAAAACCGG+AGG + chr8_4:26648213-26648232 Msa1321270:CDS 40.0%
TGAAAGAAAAACCGGAGGAA+AGG + chr8_4:26648218-26648237 Msa1321270:CDS 40.0%
TTACAACCAAACCTTTCCTC+CGG - chr8_4:26648232-26648251 None:intergenic 40.0%
! GAAAGGTTTGGTTGTAAAGC+TGG + chr8_4:26648235-26648254 Msa1321270:CDS 40.0%
! GAGTGCTCTGGTTTATGTAA+TGG - chr8_4:26648331-26648350 None:intergenic 40.0%
! GCATGGAGAATCTTCCTTTT+CGG + chr8_4:26648470-26648489 Msa1321270:CDS 40.0%
! GAGTACATTTTGAACTGCCT+AGG + chr8_4:26648882-26648901 Msa1321270:intron 40.0%
! ATTTTCTCGCTTCTCCCATT+TGG + chr8_4:26648937-26648956 Msa1321270:intron 40.0%
! CTTCTCCCATTTGGTTTCAT+GGG + chr8_4:26648946-26648965 Msa1321270:intron 40.0%
! TTCTCCCATTTGGTTTCATG+GGG + chr8_4:26648947-26648966 Msa1321270:intron 40.0%
CGATCACACAAGACAATTTC+AGG - chr8_4:26649081-26649100 None:intergenic 40.0%
ACAAAGCCATGAAAAACACC+TGG - chr8_4:26649548-26649567 None:intergenic 40.0%
! GAAGGCCTACTTCAGATTTT+AGG + chr8_4:26649719-26649738 Msa1321270:CDS 40.0%
! AGCCACCTAAAATCTGAAGT+AGG - chr8_4:26649727-26649746 None:intergenic 40.0%
!!! GTATGCATTGGTTTTCAGCT+TGG + chr8_4:26649767-26649786 Msa1321270:CDS 40.0%
! GTCAAGTCACATTTTACAGC+TGG + chr8_4:26649790-26649809 Msa1321270:CDS 40.0%
AATCGAACAAGTGAATGGTC+TGG - chr8_4:26649874-26649893 None:intergenic 40.0%
GCATTTATTCACAGGCAGTA+TGG + chr8_4:26650042-26650061 Msa1321270:CDS 40.0%
CATGTCGTATGTTTGAGAAC+AGG + chr8_4:26650310-26650329 Msa1321270:intron 40.0%
TGTCGTATGTTTGAGAACAG+GGG + chr8_4:26650312-26650331 Msa1321270:intron 40.0%
TTGAAGATCTTGGAGTTCCT+AGG + chr8_4:26650354-26650373 Msa1321270:intron 40.0%
CTAAAATCGACCATCCATGT+TGG + chr8_4:26650468-26650487 Msa1321270:CDS 40.0%
! TACACGTTTTTGTGCAGATC+CGG + chr8_4:26650554-26650573 Msa1321270:CDS 40.0%
AGAAAAACTTCAGATGCCTC+CGG - chr8_4:26650576-26650595 None:intergenic 40.0%
GAGGAGTTGCTTCTTTCTAA+AGG + chr8_4:26650627-26650646 Msa1321270:CDS 40.0%
!! TCACTGATTCATGGTGAGAG+TGG + chr8_4:26647875-26647894 Msa1321270:CDS 45.0%
GAAAAACCGGAGGAAAGGTT+TGG + chr8_4:26648223-26648242 Msa1321270:CDS 45.0%
GAAGTTATGGGTCAAGGAGT+TGG + chr8_4:26648445-26648464 Msa1321270:CDS 45.0%
! CGTCAAAAAGAGATGGTGGT+TGG + chr8_4:26648526-26648545 Msa1321270:CDS 45.0%
TGTCTTCTGCAGCATCAACT+TGG - chr8_4:26648621-26648640 None:intergenic 45.0%
! GCTTCTCCCATTTGGTTTCA+TGG + chr8_4:26648945-26648964 Msa1321270:intron 45.0%
!! ATGGCTTTGTTCATGGCACA+TGG + chr8_4:26649558-26649577 Msa1321270:intron 45.0%
! GGCCTACTTCAGATTTTAGG+TGG + chr8_4:26649722-26649741 Msa1321270:CDS 45.0%
GAGGGATAAGCATGCTGAAT+TGG + chr8_4:26649927-26649946 Msa1321270:CDS 45.0%
GCATGCTGAATTGGACTTCA+AGG + chr8_4:26649936-26649955 Msa1321270:CDS 45.0%
ATCCTAACAGTCAGCTGATG+CGG + chr8_4:26649986-26650005 Msa1321270:CDS 45.0%
TATGAGGAGGACACGACAAA+AGG + chr8_4:26650084-26650103 Msa1321270:CDS 45.0%
ATGAGGAGGACACGACAAAA+GGG + chr8_4:26650085-26650104 Msa1321270:CDS 45.0%
AAGATCTTGGAGTTCCTAGG+TGG + chr8_4:26650357-26650376 Msa1321270:intron 45.0%
GATATTGCACATGACCACCT+AGG - chr8_4:26650374-26650393 None:intergenic 45.0%
TAGACGGTGTTCTTGAAGCT+GGG + chr8_4:26650595-26650614 Msa1321270:CDS 45.0%
TGAAGCTGGGAAAAAAGCAG+AGG + chr8_4:26650608-26650627 Msa1321270:CDS 45.0%
CTAAGGTAACACGAGTGCTC+TGG - chr8_4:26648343-26648362 None:intergenic 50.0%
GTTATGGGTCAAGGAGTTGG+AGG + chr8_4:26648448-26648467 Msa1321270:CDS 50.0%
GTCTTCAGTGCCAACATGGA+TGG - chr8_4:26650481-26650500 None:intergenic 50.0%
TGGTGTCTTCAGTGCCAACA+TGG - chr8_4:26650485-26650504 None:intergenic 50.0%
! ACGTTTTTGTGCAGATCCGG+AGG + chr8_4:26650557-26650576 Msa1321270:CDS 50.0%
CTAGACGGTGTTCTTGAAGC+TGG + chr8_4:26650594-26650613 Msa1321270:CDS 50.0%
GACCGCATCAGCTGACTGTT+AGG - chr8_4:26649991-26650010 None:intergenic 55.0%
CACGACAAAAGGGTCTGCGT+TGG + chr8_4:26650095-26650114 Msa1321270:CDS 55.0%
GGGTCAAGGAGTTGGAGGCA+TGG + chr8_4:26648453-26648472 Msa1321270:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 26647821 26650662 26647821 ID=Msa1321270;Name=Msa1321270
chr8_4 mRNA 26647821 26650662 26647821 ID=Msa1321270-mRNA-1;Parent=Msa1321270;Name=Msa1321270-mRNA-1;_AED=0.14;_eAED=0.21;_QI=0|0|0|1|1|1|4|0|620
chr8_4 exon 26647821 26648547 26647821 ID=Msa1321270-mRNA-1:exon:9081;Parent=Msa1321270-mRNA-1
chr8_4 exon 26648635 26648751 26648635 ID=Msa1321270-mRNA-1:exon:9082;Parent=Msa1321270-mRNA-1
chr8_4 exon 26649584 26650315 26649584 ID=Msa1321270-mRNA-1:exon:9083;Parent=Msa1321270-mRNA-1
chr8_4 exon 26650376 26650662 26650376 ID=Msa1321270-mRNA-1:exon:9084;Parent=Msa1321270-mRNA-1
chr8_4 CDS 26647821 26648547 26647821 ID=Msa1321270-mRNA-1:cds;Parent=Msa1321270-mRNA-1
chr8_4 CDS 26648635 26648751 26648635 ID=Msa1321270-mRNA-1:cds;Parent=Msa1321270-mRNA-1
chr8_4 CDS 26649584 26650315 26649584 ID=Msa1321270-mRNA-1:cds;Parent=Msa1321270-mRNA-1
chr8_4 CDS 26650376 26650662 26650376 ID=Msa1321270-mRNA-1:cds;Parent=Msa1321270-mRNA-1
Gene Sequence

>Msa1321270

ATGGATTTGATATCAACTTCATCTGAGAGTGGACAACTGAGTGTTGAAACTTCATCACTGATTCATGGTGAGAGTGGACAATTGAGTGTTGAAAGTTCTTCAATGATTCATGCTGACAGTTCTCAATGTTACTCCCCTAGCAATAAAGAATCATGGAAGTCTTTAGTCTTTCAATCTATTAAAGAGATTGAAGATTTTTATGGTAATTATGCATATAACACTGAATTTTCTATTAGGACTATGTTGAAGTCAAAGAATAATTCACAAAATAAGAAATCTGATAAGGTACACTATGTGTGTTATGTTTGTAACAAACAAGGTTTCAAGAAGGGAAGTTTGCTTAATCCTAACAACAGGACAACATCAGACAATCCATTGGTTATTGAGTTTGTGAAAGTGAAAGAAAAACCGGAGGAAAGGTTTGGTTGTAAAGCTGGAATATATTTGAAGCTGGATGAAGTTTTGAATGTGTATAAGATATATAGATGGGATGTGGCTCACTGTCATCCATTACATAAACCAGAGCACTCGTGTTACCTTAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTAGCTCTTATAAATTCTAAAGCTGGAATGTCGATGAGAACGTCTTATGAAGTTATGGGTCAAGGAGTTGGAGGCATGGAGAATCTTCCTTTTCGGTTTTCAGACTTAAAGAACTATCTAATGACAATTCGTCAAAAAGAGATGGTGGTTGAAGACATAGCTAGTATTTTTTGGGCAAATGGAATTATGCAACTAGATTATTCTCTATTCGGCGATGTCATCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGAGAATGCAAAGAAAAATCTTGGCTCTCGTGCAAACAGTGCATTTTTTGATGAGTTAACTAATTTGGTTTCAAATGTAGATGATGAATCAGATTTTGACTATAATTGGGATCAGATGATGAAAAATTGTTTTAATGGAAGGCCTACTTCAGATTTTAGGTGGCTTGTCCAAACTTACGAAAATCGTATGCATTGGTTTTCAGCTTGGGTCAAGTCACATTTTACAGCTGGTCTGAAAATGACACAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTCTGCTGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATAGTTCAGAGAATGAGGGATAAGCATGCTGAATTGGACTTCAAGGCTGCAAACACTAGAACAAAAAATAATTATCCTAACAGTCAGCTGATGCGGTCCGTTGTAAACAAGTACACACCAGCTTGCTTTGCATTTATTCACAGGCAGTATGGCCTTTCCTTCAAATACTATTATGAGGAGGACACGACAAAAGGGTCTGCGTTGGAGAAGTTTTTCAAAGTTTTCACAATTGAAAGGGTTGATGACAATTATGATGATGCTGATAATGACGGGTCTTCCAATGTTGATGTTTTAGATGCAGAACTTCAAGAAAATGTTTTCCCAAGTTTTGAAGATCATGATCGATTTGATGAAAGGCTTGTCACAGTTGACATTAGAAGCAAAAGTTTTAGTTGTTCATGTCGTGGTCATGTGCAATATCATTTTTTGAAGACAATACCTGGACAATATGTTTTAAAGCGTTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTAAAATCGACCATCCATGTTGGCACTGAAGACACCAATCAAGCACAACGATATCAACAAATTTGTGATGTTACAGTTCAGCTTTCTACACGTTTTTGTGCAGATCCGGAGGCATCTGAAGTTTTTCTAGACGGTGTTCTTGAAGCTGGGAAAAAAGCAGAGGAGTTGCTTCTTTCTAAAGGTATACGTACATAA
Protein sequence

>Msa1321270

MDLISTSSESGQLSVETSSLIHGESGQLSVESSSMIHADSSQCYSPSNKESWKSLVFQSIKEIEDFYGNYAYNTEFSIRTMLKSKNNSQNKKSDKVHYVCYVCNKQGFKKGSLLNPNNRTTSDNPLVIEFVKVKEKPEERFGCKAGIYLKLDEVLNVYKIYRWDVAHCHPLHKPEHSCYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGMENLPFRFSDLKNYLMTIRQKEMVVEDIASIFWANGIMQLDYSLFGDVISFDTTYRTNNKYRPLENAKKNLGSRANSAFFDELTNLVSNVDDESDFDYNWDQMMKNCFNGRPTSDFRWLVQTYENRMHWFSAWVKSHFTAGLKMTQLSESFNAFLRGFLLPDHSLVRFFSHFNIIVQRMRDKHAELDFKAANTRTKNNYPNSQLMRSVVNKYTPACFAFIHRQYGLSFKYYYEEDTTKGSALEKFFKVFTIERVDDNYDDADNDGSSNVDVLDAELQENVFPSFEDHDRFDERLVTVDIRSKSFSCSCRGHVQYHFLKTIPGQYVLKRWTRDVRPSVDKLKSTIHVGTEDTNQAQRYQQICDVTVQLSTRFCADPEASEVFLDGVLEAGKKAEELLLSKGIRT*