AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1322080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1322080 MtrunA17_Chr2g0296781 75.730 651 154 3 140 788 36 684 0.0 1023
Msa1322080 MtrunA17_Chr6g0477261 77.178 574 128 2 217 788 1 573 0.0 913
Msa1322080 MtrunA17_Chr1g0196541 64.985 654 220 3 140 791 36 682 0.0 866
Msa1322080 MtrunA17_Chr5g0441581 44.279 673 334 10 110 754 21 680 0.0 555
Msa1322080 MtrunA17_Chr2g0291581 37.225 591 332 10 94 660 47 622 4.86e-123 385
Msa1322080 MtrunA17_Chr1g0207551 39.235 497 291 4 168 661 103 591 1.38e-118 377
Msa1322080 MtrunA17_Chr5g0448761 38.269 520 301 8 155 664 121 630 2.55e-118 375
Msa1322080 MtrunA17_Chr1g0202341 37.238 572 322 11 115 660 50 610 9.17e-118 371
Msa1322080 MtrunA17_Chr2g0329031 36.994 519 303 8 156 668 70 570 9.23e-113 360
Msa1322080 MtrunA17_Chr3g0080351 37.597 516 288 7 149 661 141 625 1.60e-109 354
Msa1322080 MtrunA17_Chr2g0291591 33.586 527 327 5 85 591 33 556 3.48e-99 319
Msa1322080 MtrunA17_Chr5g0448511 36.919 409 248 4 259 664 1 402 3.12e-90 295
Msa1322080 MtrunA17_Chr5g0440451 31.872 593 351 13 115 674 42 614 2.92e-87 296
Msa1322080 MtrunA17_Chr3g0118611 38.776 392 229 4 271 659 118 501 4.22e-86 290
Msa1322080 MtrunA17_Chr2g0287651 29.455 550 374 6 168 713 147 686 1.45e-81 279
Msa1322080 MtrunA17_Chr8g0363841 32.200 500 321 10 168 659 88 577 8.02e-77 262
Msa1322080 MtrunA17_Chr2g0324011 28.715 599 379 12 113 680 7 588 1.87e-76 259
Msa1322080 MtrunA17_Chr2g0287061 30.838 561 327 12 115 657 182 699 2.72e-75 261
Msa1322080 MtrunA17_Chr6g0476441 68.947 190 58 1 599 788 1 189 3.98e-74 243
Msa1322080 MtrunA17_Chr1g0198761 28.487 674 408 20 123 766 85 714 3.07e-71 248
Msa1322080 MtrunA17_Chr2g0276841 29.402 619 373 17 138 725 112 697 4.96e-71 247
Msa1322080 MtrunA17_Chr1g0203051 28.600 500 334 8 149 640 132 616 6.45e-68 237
Msa1322080 MtrunA17_Chr1g0203721 28.261 552 370 9 120 659 86 623 1.08e-64 232
Msa1322080 MtrunA17_Chr6g0458391 27.711 581 366 13 105 659 213 765 1.72e-64 230
Msa1322080 MtrunA17_Chr3g0082121 30.713 547 350 16 120 659 66 590 1.65e-60 218
Msa1322080 MtrunA17_Chr2g0294841 28.266 467 318 6 194 655 150 604 2.24e-57 209
Msa1322080 MtrunA17_Chr5g0431001 32.907 313 197 5 194 498 168 475 4.73e-51 186
Msa1322080 MtrunA17_Chr1g0164591 33.442 308 193 6 183 487 10 308 3.51e-48 173
Msa1322080 MtrunA17_Chr2g0290391 25.532 611 384 18 92 657 27 611 1.31e-47 180
Msa1322080 MtrunA17_Chr7g0258861 31.707 287 175 3 109 374 3 289 1.71e-40 151
Msa1322080 MtrunA17_Chr2g0308841 24.675 231 159 3 431 656 4 224 3.29e-19 88.6
Msa1322080 MtrunA17_Chr6g0476431 60.784 51 20 0 140 190 36 86 1.67e-15 72.4
Msa1322080 MtrunA17_Chr1g0172041 32.727 110 73 1 571 680 1 109 3.71e-13 66.6
Msa1322080 MtrunA17_Chr6g0451441 30.328 122 82 2 142 263 129 247 3.84e-13 72.8
Msa1322080 MtrunA17_Chr3g0077731 57.143 77 32 1 712 788 1 76 1.06e-12 65.5
Msa1322080 MtrunA17_Chr1g0149131 28.448 116 68 2 376 482 1 110 2.71e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1322080 AT4G15090.1 36.145 581 348 10 168 736 122 691 3.22e-120 382
Msa1322080 AT4G15090.5 36.145 581 348 10 168 736 122 691 3.22e-120 382
Msa1322080 AT4G15090.3 36.145 581 348 10 168 736 122 691 3.22e-120 382
Msa1322080 AT4G15090.2 36.145 581 348 10 168 736 122 691 3.22e-120 382
Msa1322080 AT4G15090.4 36.145 581 348 10 168 736 122 691 3.22e-120 382
Msa1322080 AT4G38180.1 35.827 508 316 4 155 659 134 634 8.45e-113 362
Msa1322080 AT1G52520.1 34.338 597 344 14 112 679 81 658 5.18e-110 352
Msa1322080 AT3G22170.2 37.524 517 290 7 149 662 135 621 1.43e-108 352
Msa1322080 AT3G22170.1 37.524 517 290 7 149 662 135 621 1.43e-108 352
Msa1322080 AT1G76320.3 36.293 518 308 7 156 668 53 553 5.57e-104 335
Msa1322080 AT1G76320.1 36.293 518 308 7 156 668 53 553 1.18e-103 336
Msa1322080 AT1G76320.4 36.293 518 308 7 156 668 53 553 1.18e-103 336
Msa1322080 AT1G76320.2 36.293 518 308 7 156 668 53 553 1.48e-103 335
Msa1322080 AT1G80010.1 33.943 601 344 18 103 663 78 665 1.86e-97 319
Msa1322080 AT4G19990.1 33.966 527 293 9 143 664 31 507 2.32e-89 296
Msa1322080 AT4G19990.3 33.966 527 293 9 143 664 88 564 4.33e-89 297
Msa1322080 AT4G19990.4 33.966 527 293 9 143 664 88 564 4.33e-89 297
Msa1322080 AT4G19990.5 33.966 527 293 9 143 664 88 564 6.81e-89 297
Msa1322080 AT4G19990.6 33.966 527 293 9 143 664 88 564 8.79e-89 297
Msa1322080 AT4G19990.2 33.966 527 293 9 143 664 88 564 8.79e-89 297
Msa1322080 AT4G19990.7 33.966 527 293 9 143 664 102 578 8.87e-89 297
Msa1322080 AT2G27110.2 32.149 591 366 10 91 668 20 588 1.02e-87 296
Msa1322080 AT2G27110.1 32.149 591 366 10 91 668 20 588 1.02e-87 296
Msa1322080 AT2G27110.3 34.529 446 273 5 226 668 14 443 2.81e-83 281
Msa1322080 AT2G32250.8 37.696 382 228 3 278 656 135 509 1.02e-81 278
Msa1322080 AT2G32250.5 37.696 382 228 3 278 656 176 550 1.19e-81 278
Msa1322080 AT2G32250.7 37.696 382 228 3 278 656 135 509 1.24e-81 278
Msa1322080 AT2G32250.6 37.696 382 228 3 278 656 176 550 1.48e-81 278
Msa1322080 AT2G32250.1 37.696 382 228 3 278 656 176 550 1.48e-81 278
Msa1322080 AT2G32250.3 37.696 382 228 3 278 656 176 550 1.48e-81 278
Msa1322080 AT2G32250.2 37.696 382 228 3 278 656 176 550 1.58e-81 278
Msa1322080 AT2G32250.4 37.696 382 228 3 278 656 176 550 1.58e-81 278
Msa1322080 AT4G38170.1 34.937 395 243 5 273 663 19 403 3.14e-77 260
Msa1322080 AT3G06250.2 29.706 579 344 13 106 668 180 711 1.48e-76 264
Msa1322080 AT3G06250.1 29.706 579 344 13 106 668 180 711 1.48e-76 264
Msa1322080 AT3G06250.3 29.706 579 344 13 106 668 180 711 1.48e-76 264
Msa1322080 AT5G18960.2 31.127 559 327 16 115 657 210 726 1.79e-74 258
Msa1322080 AT5G18960.1 31.127 559 327 16 115 657 210 726 1.79e-74 258
Msa1322080 AT1G10240.2 28.991 545 331 15 138 656 90 604 8.76e-65 229
Msa1322080 AT1G10240.1 28.991 545 331 15 138 656 90 604 8.76e-65 229
Msa1322080 AT5G28530.3 24.922 642 411 19 116 711 55 671 1.32e-46 177
Msa1322080 AT5G28530.2 24.922 642 411 19 116 711 55 671 1.32e-46 177
Msa1322080 AT5G28530.1 24.923 646 410 20 116 711 55 675 1.03e-45 175
Msa1322080 AT5G28530.5 26.649 379 245 12 344 711 252 608 9.45e-28 119
Msa1322080 AT5G28530.4 26.632 383 244 13 344 711 252 612 6.98e-27 117

Find 106 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTGTTTATTTGAGTTTA+AGG 0.126982 8_4:-28659196 Msa1322080:CDS
ATTGCTTGTATAAGTCATTT+TGG 0.199496 8_4:-28659435 Msa1322080:CDS
TCCATCCTGGTCAGTAATTA+TGG 0.258712 8_4:+28659902 None:intergenic
TTTGAGCATAAGTGATTTCA+TGG 0.262311 8_4:-28659736 Msa1322080:CDS
TGTATGTGAGAGACACATTT+TGG 0.270477 8_4:-28659623 Msa1322080:CDS
CACCATCGATGGCGAGCTTT+CGG 0.279320 8_4:+28659849 None:intergenic
GGAAATCAAATTTCAATTGA+TGG 0.294248 8_4:+28661011 None:intergenic
TGGAACAATTACTATTTCTA+AGG 0.295449 8_4:-28660504 Msa1322080:CDS
TAAAATTGAAAAGGAAAATA+TGG 0.301034 8_4:-28659484 Msa1322080:CDS
TATACAAGCAATAATTCTAT+TGG 0.306437 8_4:+28659446 None:intergenic
TAGTATCCTCCTTCGACAAT+AGG 0.312589 8_4:+28659985 None:intergenic
AGGGCTGCTTATGAGTATTT+TGG 0.315150 8_4:-28660116 Msa1322080:CDS
TTGAACTGGATGCTAAAATA+AGG 0.316532 8_4:+28660990 None:intergenic
TGAAGAAGCAAATCCGTAAA+AGG 0.325895 8_4:+28658990 None:intergenic
TGCTGTTTATTTGAGTTTAA+GGG 0.334665 8_4:-28659195 Msa1322080:CDS
CTAAAATATCTTGATTTGCT+TGG 0.342688 8_4:+28660449 None:intergenic
ATATAGACCCTAGTATAAAA+AGG 0.351811 8_4:-28660415 Msa1322080:CDS
ACGCTGCAACTCTTCATTTC+CGG 0.353530 8_4:+28659032 None:intergenic
GAACAAAATCAGAACATATA+AGG 0.353968 8_4:+28661137 None:intergenic
TTTCTTGGTTGTTCACTACT+TGG 0.357896 8_4:-28659956 Msa1322080:CDS
CTTACTATTGTCTCTTGAAC+TGG 0.373416 8_4:+28660976 None:intergenic
TCATGCTTTCTTTGAGAGAA+TGG 0.375982 8_4:-28659331 Msa1322080:CDS
ACCATCGATGGCGAGCTTTC+GGG 0.381382 8_4:+28659850 None:intergenic
TTGTGTTACAGAAAGTAAAA+AGG 0.383201 8_4:-28659289 Msa1322080:CDS
CATGGTCAGTCAGTTCTGTT+AGG 0.383640 8_4:-28660014 Msa1322080:CDS
CTTGTGGCATATAATGAAAA+AGG 0.386165 8_4:-28659826 Msa1322080:CDS
TAGAATAGAGAACTTAAAAC+TGG 0.390920 8_4:-28658929 Msa1322080:CDS
AGTATCCTCCTTCGACAATA+GGG 0.392582 8_4:+28659986 None:intergenic
GGATATGATAACCTTACATT+CGG 0.406034 8_4:-28660320 Msa1322080:CDS
AATCAGAACATATAAGGTTA+TGG 0.408232 8_4:+28661143 None:intergenic
CCTTTACTTCGAGTCGCTAC+AGG 0.409218 8_4:+28658826 None:intergenic
AAGGGCGTCCTCCTTCAAAA+AGG 0.415773 8_4:-28658807 Msa1322080:CDS
TTAAATTTCATGCCAACCTT+AGG 0.424975 8_4:+28660750 None:intergenic
GTTTGATGAGCGTCATCGTT+GGG 0.431278 8_4:-28659652 Msa1322080:CDS
AAGTGAGGCGGCTTCGACTT+GGG 0.432744 8_4:-28660268 Msa1322080:CDS
ATATTTATTTGTTAAGTAAG+TGG 0.436838 8_4:+28660073 None:intergenic
TTAATATCCTTCCTCCATCT+TGG 0.438245 8_4:+28659096 None:intergenic
CTTGTTATATATTGCCAAGA+TGG 0.439590 8_4:-28659110 Msa1322080:CDS
AAGAAGCTTGCTGGAAAGAA+AGG 0.445180 8_4:-28658754 Msa1322080:CDS
CATTCTTCATTGCTCCATCC+TGG 0.449843 8_4:+28659889 None:intergenic
CATGATAATGAATGGTTGAA+AGG 0.452805 8_4:-28659678 Msa1322080:CDS
GATATGATAACCTTACATTC+GGG 0.456870 8_4:-28660319 Msa1322080:CDS
ATTTGAATCTCAATTTCAAA+AGG 0.459389 8_4:-28659412 Msa1322080:CDS
CAAATTTCAATTGATGGTTG+AGG 0.460215 8_4:+28661017 None:intergenic
ACTCCAACAAAAGGAGCAAA+AGG 0.476605 8_4:+28660044 None:intergenic
CAAAGAGTTTCAAGTAGAAG+TGG 0.481571 8_4:-28659370 Msa1322080:CDS
TTTGGGCAGATGCGAGATGT+AGG 0.485083 8_4:-28660136 Msa1322080:CDS
AATTAAAGACATAATGTTCA+AGG 0.486667 8_4:-28659256 Msa1322080:CDS
TACTTACATTAATCCCAGCT+TGG 0.488151 8_4:+28660375 None:intergenic
CCTGTAGCGACTCGAAGTAA+AGG 0.488591 8_4:-28658826 Msa1322080:CDS
TAGCATGAAAACACACTGTA+AGG 0.490277 8_4:-28660555 Msa1322080:CDS
CTGTAGCGACTCGAAGTAAA+GGG 0.498287 8_4:-28658825 Msa1322080:CDS
TCTATGGTTGTTGAAGCAAA+TGG 0.501556 8_4:-28660341 Msa1322080:CDS
ATTGAAAGATAAAATTGAAA+AGG 0.501824 8_4:-28659493 Msa1322080:CDS
TCCCGAAAGCTCGCCATCGA+TGG 0.503126 8_4:-28659851 Msa1322080:CDS
TTTGTTGGAGTAAATCATCA+TGG 0.506547 8_4:-28660032 Msa1322080:CDS
AGGCGGCTTCGACTTGGGAC+AGG 0.507987 8_4:-28660263 Msa1322080:CDS
TTGGGCAGATGCGAGATGTA+GGG 0.512318 8_4:-28660135 Msa1322080:CDS
TGATGATTTACTCCAACAAA+AGG 0.515106 8_4:+28660035 None:intergenic
TGTTTGATGAGCGTCATCGT+TGG 0.518510 8_4:-28659653 Msa1322080:CDS
AAAGTGAGGCGGCTTCGACT+TGG 0.518819 8_4:-28660269 Msa1322080:CDS
GACGACAACGAGTTTGAAAA+CGG 0.527121 8_4:+28661079 None:intergenic
CGGCGGTGAATGTTGGTAAC+CGG 0.527849 8_4:+28661099 None:intergenic
TTGGAGGTTAATGACCAAGC+TGG 0.528235 8_4:-28660389 Msa1322080:CDS
ATGATAATGAATGGTTGAAA+GGG 0.531139 8_4:-28659677 Msa1322080:CDS
AAATGATTTATCAAAAGTAA+TGG 0.531224 8_4:-28658953 Msa1322080:CDS
AACATATAAGGTTATGGAGA+AGG 0.534401 8_4:+28661149 None:intergenic
TGGAGGTTAATGACCAAGCT+GGG 0.539674 8_4:-28660388 Msa1322080:CDS
CTCGCCATCGATGGTGCTTG+TGG 0.540244 8_4:-28659842 Msa1322080:CDS
TACTCACATAGCTTCTTGCA+CGG 0.540398 8_4:+28660606 None:intergenic
ATGAACTTCATGATAATGAA+TGG 0.543870 8_4:-28659686 Msa1322080:CDS
AAATGAAGATGATGAACCTA+AGG 0.544931 8_4:-28660766 Msa1322080:CDS
TTGAAAACGGCGGTGAATGT+TGG 0.547831 8_4:+28661092 None:intergenic
AAGTGATTTCATGGAGAAGT+GGG 0.549527 8_4:-28659727 Msa1322080:CDS
GAAGATGATGAACCTAAGGT+TGG 0.554642 8_4:-28660762 Msa1322080:CDS
AAATCCGTAAAAGGCATCGC+AGG 0.562477 8_4:+28658999 None:intergenic
TAAGTGATTTCATGGAGAAG+TGG 0.562788 8_4:-28659728 Msa1322080:CDS
GTTCAACAACACAAACTCAC+CGG 0.564921 8_4:-28661118 Msa1322080:CDS
ATGGCGAGCTTTCGGGAAGA+CGG 0.568527 8_4:+28659857 None:intergenic
AATGTATGTGTGTCTTCAGA+TGG 0.571681 8_4:-28660524 Msa1322080:CDS
TGGTCAGTAATTATGGCTTG+TGG 0.579259 8_4:+28659909 None:intergenic
TGCTTTCTTTGAGAGAATGG+AGG 0.581817 8_4:-28659328 Msa1322080:CDS
GTTATATATTGCCAAGATGG+AGG 0.585753 8_4:-28659107 Msa1322080:CDS
AGGTTGCGCCCTATTGTCGA+AGG 0.587751 8_4:-28659994 Msa1322080:CDS
TGTTAAGTAAGTGGTGTCGA+AGG 0.588670 8_4:+28660082 None:intergenic
GAAACTACATTGACAAAGTG+AGG 0.593331 8_4:-28660283 Msa1322080:CDS
TATATTGCCAAGATGGAGGA+AGG 0.599926 8_4:-28659103 Msa1322080:CDS
CACTTATCAAAGTCAAGAAG+AGG 0.608354 8_4:-28658707 Msa1322080:CDS
TCTTGGCAATATATAACAAG+AGG 0.613918 8_4:+28659113 None:intergenic
CATGAAATTTAATTCTACAG+AGG 0.616241 8_4:-28660739 Msa1322080:CDS
ACTTGGTTAGAATGTATGCG+TGG 0.623148 8_4:-28659939 Msa1322080:CDS
ACAAGCCATAATTACTGACC+AGG 0.626330 8_4:-28659907 Msa1322080:CDS
AGCAATGAAGAATGCAGTTG+AGG 0.631682 8_4:-28659880 Msa1322080:CDS
AATGTGTCTCTCACATACAC+AGG 0.631924 8_4:+28659627 None:intergenic
GCCATAATTACTGACCAGGA+TGG 0.636449 8_4:-28659903 Msa1322080:CDS
CTTGGTTAGAATGTATGCGT+GGG 0.645599 8_4:-28659938 Msa1322080:CDS
CTTGGCAATATATAACAAGA+GGG 0.647143 8_4:+28659114 None:intergenic
TCACATAAATGAAAATTGTG+AGG 0.656952 8_4:-28660802 Msa1322080:CDS
TATGCCACAAGCACCATCGA+TGG 0.658002 8_4:+28659838 None:intergenic
TTGCGCCCTATTGTCGAAGG+AGG 0.658239 8_4:-28659991 Msa1322080:CDS
ATATATGGAAGAGACAATCA+CGG 0.693219 8_4:-28661209 None:intergenic
CTGTGAGCTTAAGAACACAA+AGG 0.694006 8_4:+28659154 None:intergenic
GTAGCAATGCTGATAAACAG+AGG 0.712390 8_4:+28661045 None:intergenic
ATATGATAACCTTACATTCG+GGG 0.714729 8_4:-28660318 Msa1322080:CDS
ACTACATTGACAAAGTGAGG+CGG 0.739143 8_4:-28660280 Msa1322080:CDS
GACAACGAGTTTGAAAACGG+CGG 0.794970 8_4:+28661082 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAGTATTTTACTCTA+GGG - chr8_4:28659234-28659253 Msa1322080:CDS 15.0%
!!! TTAATGTAAGTAGAAATTTT+CGG - chr8_4:28659504-28659523 Msa1322080:CDS 15.0%
!!! TCTAACAAAATAATTTTGAA+TGG + chr8_4:28659642-28659661 None:intergenic 15.0%
!!! TAGTGAATTTTATTATGTAA+TGG - chr8_4:28659676-28659695 Msa1322080:CDS 15.0%
!!! ATATTTATTTGTTAAGTAAG+TGG + chr8_4:28659798-28659817 None:intergenic 15.0%
!! ATTGAAAGATAAAATTGAAA+AGG - chr8_4:28660375-28660394 Msa1322080:CDS 15.0%
!! TAAAATTGAAAAGGAAAATA+TGG - chr8_4:28660384-28660403 Msa1322080:CDS 15.0%
!! AAATGATTTATCAAAAGTAA+TGG - chr8_4:28660915-28660934 Msa1322080:intron 15.0%
!! ATCAACTGAATTAAAAGTAA+AGG + chr8_4:28658962-28658981 None:intergenic 20.0%
!!! TAAAAAATTTGTTGCATTAG+TGG - chr8_4:28659000-28659019 Msa1322080:CDS 20.0%
!!! GAAAAAAAGTATTTTACTCT+AGG - chr8_4:28659233-28659252 Msa1322080:CDS 20.0%
!!! ATTTGGCTTTAAAAGATTTT+TGG + chr8_4:28659291-28659310 None:intergenic 20.0%
!!! ATTTTATTATGTAATGGATG+TGG - chr8_4:28659682-28659701 Msa1322080:CDS 20.0%
!! TATACAAGCAATAATTCTAT+TGG + chr8_4:28660425-28660444 None:intergenic 20.0%
!! ATTTGAATCTCAATTTCAAA+AGG - chr8_4:28660456-28660475 Msa1322080:CDS 20.0%
!! AATTAAAGACATAATGTTCA+AGG - chr8_4:28660612-28660631 Msa1322080:CDS 20.0%
!!! TTGATAAATCATTTTCAGTA+TGG + chr8_4:28660909-28660928 None:intergenic 20.0%
! AATCAGAACATATAAGGTTA+TGG + chr8_4:28658728-28658747 None:intergenic 25.0%
! GAACAAAATCAGAACATATA+AGG + chr8_4:28658734-28658753 None:intergenic 25.0%
! GGAAATCAAATTTCAATTGA+TGG + chr8_4:28658860-28658879 None:intergenic 25.0%
! TCACATAAATGAAAATTGTG+AGG - chr8_4:28659066-28659085 Msa1322080:CDS 25.0%
! CATGAAATTTAATTCTACAG+AGG - chr8_4:28659129-28659148 Msa1322080:CDS 25.0%
! AAGAATTATGCAAGATGTTT+AGG - chr8_4:28659169-28659188 Msa1322080:CDS 25.0%
!!! TGGAACAATTACTATTTCTA+AGG - chr8_4:28659364-28659383 Msa1322080:CDS 25.0%
!!! CTAAAATATCTTGATTTGCT+TGG + chr8_4:28659422-28659441 None:intergenic 25.0%
! ATATAGACCCTAGTATAAAA+AGG - chr8_4:28659453-28659472 Msa1322080:CDS 25.0%
!!! TCGTTTACGAAATGTTTTTT+GGG - chr8_4:28659715-28659734 Msa1322080:CDS 25.0%
!!! AGGATACTAAAACTTTTTCT+TGG - chr8_4:28659897-28659916 Msa1322080:CDS 25.0%
!! ATGAACTTCATGATAATGAA+TGG - chr8_4:28660182-28660201 Msa1322080:CDS 25.0%
!! ATGATAATGAATGGTTGAAA+GGG - chr8_4:28660191-28660210 Msa1322080:CDS 25.0%
!! ATTGCTTGTATAAGTCATTT+TGG - chr8_4:28660433-28660452 Msa1322080:CDS 25.0%
!! AAACTCTTTGAATTTTGCAT+TGG + chr8_4:28660488-28660507 None:intergenic 25.0%
! TTGTGTTACAGAAAGTAAAA+AGG - chr8_4:28660579-28660598 Msa1322080:CDS 25.0%
!! ATGCTGTTTATTTGAGTTTA+AGG - chr8_4:28660672-28660691 Msa1322080:intron 25.0%
!! TGCTGTTTATTTGAGTTTAA+GGG - chr8_4:28660673-28660692 Msa1322080:intron 25.0%
!!! AATGTTTTTGATCAATTAGC+CGG - chr8_4:28660817-28660836 Msa1322080:CDS 25.0%
! TAGAATAGAGAACTTAAAAC+TGG - chr8_4:28660939-28660958 Msa1322080:intron 25.0%
AACATATAAGGTTATGGAGA+AGG + chr8_4:28658722-28658741 None:intergenic 30.0%
! CAAATTTCAATTGATGGTTG+AGG + chr8_4:28658854-28658873 None:intergenic 30.0%
TTGAACTGGATGCTAAAATA+AGG + chr8_4:28658881-28658900 None:intergenic 30.0%
GAATTAAAAGTAAAGGCAAC+AGG + chr8_4:28658955-28658974 None:intergenic 30.0%
AAATGAAGATGATGAACCTA+AGG - chr8_4:28659102-28659121 Msa1322080:CDS 30.0%
TTAAATTTCATGCCAACCTT+AGG + chr8_4:28659121-28659140 None:intergenic 30.0%
!! TATGCAAGATGTTTAGGTTT+TGG - chr8_4:28659175-28659194 Msa1322080:CDS 30.0%
TAGCACAATCAGTACAAAAA+AGG - chr8_4:28659201-28659220 Msa1322080:CDS 30.0%
AGCACAATCAGTACAAAAAA+GGG - chr8_4:28659202-28659221 Msa1322080:CDS 30.0%
! CCAATCTCCTTTTTATACTA+GGG + chr8_4:28659463-28659482 None:intergenic 30.0%
! TCCAATCTCCTTTTTATACT+AGG + chr8_4:28659464-28659483 None:intergenic 30.0%
TAGTATAAAAAGGAGATTGG+AGG - chr8_4:28659463-28659482 Msa1322080:CDS 30.0%
! AAGTAGAAATTTTCGGTCTA+TGG - chr8_4:28659511-28659530 Msa1322080:CDS 30.0%
GGATATGATAACCTTACATT+CGG - chr8_4:28659548-28659567 Msa1322080:CDS 30.0%
GATATGATAACCTTACATTC+GGG - chr8_4:28659549-28659568 Msa1322080:CDS 30.0%
ATATGATAACCTTACATTCG+GGG - chr8_4:28659550-28659569 Msa1322080:CDS 30.0%
!!! GTCGTTTACGAAATGTTTTT+TGG - chr8_4:28659714-28659733 Msa1322080:CDS 30.0%
TGATGATTTACTCCAACAAA+AGG + chr8_4:28659836-28659855 None:intergenic 30.0%
TTTGTTGGAGTAAATCATCA+TGG - chr8_4:28659836-28659855 Msa1322080:CDS 30.0%
CTTGTGGCATATAATGAAAA+AGG - chr8_4:28660042-28660061 Msa1322080:CDS 30.0%
! TAGAGTCTACTCAATTTTTC+TGG + chr8_4:28660070-28660089 None:intergenic 30.0%
! CAAAATACTTTTGCATGATG+CGG - chr8_4:28660099-28660118 Msa1322080:CDS 30.0%
TTTGAGCATAAGTGATTTCA+TGG - chr8_4:28660132-28660151 Msa1322080:CDS 30.0%
! CATGATAATGAATGGTTGAA+AGG - chr8_4:28660190-28660209 Msa1322080:CDS 30.0%
!!! AGTATGAACGCATTTTTTGA+TGG - chr8_4:28660298-28660317 Msa1322080:CDS 30.0%
CTTGGCAATATATAACAAGA+GGG + chr8_4:28660757-28660776 None:intergenic 30.0%
TCTTGGCAATATATAACAAG+AGG + chr8_4:28660758-28660777 None:intergenic 30.0%
! CTTGTTATATATTGCCAAGA+TGG - chr8_4:28660758-28660777 Msa1322080:CDS 30.0%
CTTACTATTGTCTCTTGAAC+TGG + chr8_4:28658895-28658914 None:intergenic 35.0%
AGTACAAAAAAGGGAGATGA+TGG - chr8_4:28659211-28659230 Msa1322080:CDS 35.0%
! TGTGTTTTCATGCTAGGATT+TGG + chr8_4:28659308-28659327 None:intergenic 35.0%
! TTACAGTGTGTTTTCATGCT+AGG + chr8_4:28659314-28659333 None:intergenic 35.0%
TAGCATGAAAACACACTGTA+AGG - chr8_4:28659313-28659332 Msa1322080:CDS 35.0%
AATGTATGTGTGTCTTCAGA+TGG - chr8_4:28659344-28659363 Msa1322080:CDS 35.0%
CCCTAGTATAAAAAGGAGAT+TGG - chr8_4:28659460-28659479 Msa1322080:CDS 35.0%
TACTTACATTAATCCCAGCT+TGG + chr8_4:28659496-28659515 None:intergenic 35.0%
TCTATGGTTGTTGAAGCAAA+TGG - chr8_4:28659527-28659546 Msa1322080:CDS 35.0%
GAAACTACATTGACAAAGTG+AGG - chr8_4:28659585-28659604 Msa1322080:CDS 35.0%
TTTCTTGGTTGTTCACTACT+TGG - chr8_4:28659912-28659931 Msa1322080:CDS 35.0%
TAAGTGATTTCATGGAGAAG+TGG - chr8_4:28660140-28660159 Msa1322080:CDS 35.0%
AAGTGATTTCATGGAGAAGT+GGG - chr8_4:28660141-28660160 Msa1322080:CDS 35.0%
! TGTATGTGAGAGACACATTT+TGG - chr8_4:28660245-28660264 Msa1322080:CDS 35.0%
! GTATGTGAGAGACACATTTT+GGG - chr8_4:28660246-28660265 Msa1322080:CDS 35.0%
! CAAAGAGTTTCAAGTAGAAG+TGG - chr8_4:28660498-28660517 Msa1322080:CDS 35.0%
! TCATGCTTTCTTTGAGAGAA+TGG - chr8_4:28660537-28660556 Msa1322080:CDS 35.0%
GTTATATATTGCCAAGATGG+AGG - chr8_4:28660761-28660780 Msa1322080:CDS 35.0%
TTAATATCCTTCCTCCATCT+TGG + chr8_4:28660775-28660794 None:intergenic 35.0%
TGAAGAAGCAAATCCGTAAA+AGG + chr8_4:28660881-28660900 None:intergenic 35.0%
!! AGTTTTTCAGAGATCACTGA+AGG - chr8_4:28660979-28660998 Msa1322080:intron 35.0%
! TTAGAAGCCTTCCTTTTTGA+AGG + chr8_4:28661075-28661094 None:intergenic 35.0%
ATTGTGAAAAAGAAGCTTGC+TGG - chr8_4:28661105-28661124 Msa1322080:CDS 35.0%
CACTTATCAAAGTCAAGAAG+AGG - chr8_4:28661161-28661180 Msa1322080:CDS 35.0%
GTTCAACAACACAAACTCAC+CGG - chr8_4:28658750-28658769 Msa1322080:CDS 40.0%
GACGACAACGAGTTTGAAAA+CGG + chr8_4:28658792-28658811 None:intergenic 40.0%
GTAGCAATGCTGATAAACAG+AGG + chr8_4:28658826-28658845 None:intergenic 40.0%
GAAGATGATGAACCTAAGGT+TGG - chr8_4:28659106-28659125 Msa1322080:CDS 40.0%
TACTCACATAGCTTCTTGCA+CGG + chr8_4:28659265-28659284 None:intergenic 40.0%
! CATCTTTTTCCCCGAATGTA+AGG + chr8_4:28659562-28659581 None:intergenic 40.0%
ACTACATTGACAAAGTGAGG+CGG - chr8_4:28659588-28659607 Msa1322080:CDS 40.0%
! AGGGCTGCTTATGAGTATTT+TGG - chr8_4:28659752-28659771 Msa1322080:CDS 40.0%
! TGTTAAGTAAGTGGTGTCGA+AGG + chr8_4:28659789-28659808 None:intergenic 40.0%
! ATGCCTTTTGCTCCTTTTGT+TGG - chr8_4:28659821-28659840 Msa1322080:CDS 40.0%
ACTCCAACAAAAGGAGCAAA+AGG + chr8_4:28659827-28659846 None:intergenic 40.0%
AGTATCCTCCTTCGACAATA+GGG + chr8_4:28659885-28659904 None:intergenic 40.0%
TAGTATCCTCCTTCGACAAT+AGG + chr8_4:28659886-28659905 None:intergenic 40.0%
ACTTGGTTAGAATGTATGCG+TGG - chr8_4:28659929-28659948 Msa1322080:CDS 40.0%
CTTGGTTAGAATGTATGCGT+GGG - chr8_4:28659930-28659949 Msa1322080:CDS 40.0%
TGGTCAGTAATTATGGCTTG+TGG + chr8_4:28659962-28659981 None:intergenic 40.0%
ACAAGCCATAATTACTGACC+AGG - chr8_4:28659961-28659980 Msa1322080:CDS 40.0%
TCCATCCTGGTCAGTAATTA+TGG + chr8_4:28659969-28659988 None:intergenic 40.0%
AGCAATGAAGAATGCAGTTG+AGG - chr8_4:28659988-28660007 Msa1322080:CDS 40.0%
AATGTGTCTCTCACATACAC+AGG + chr8_4:28660244-28660263 None:intergenic 40.0%
! TGCTTTCTTTGAGAGAATGG+AGG - chr8_4:28660540-28660559 Msa1322080:CDS 40.0%
CTGTGAGCTTAAGAACACAA+AGG + chr8_4:28660717-28660736 None:intergenic 40.0%
TATATTGCCAAGATGGAGGA+AGG - chr8_4:28660765-28660784 Msa1322080:CDS 40.0%
!! AAGAAGCTTGCTGGAAAGAA+AGG - chr8_4:28661114-28661133 Msa1322080:CDS 40.0%
!!! CACGGCACACGTTTTGATTT+TGG - chr8_4:28658677-28658696 Msa1322080:CDS 45.0%
TTGAAAACGGCGGTGAATGT+TGG + chr8_4:28658779-28658798 None:intergenic 45.0%
GACAACGAGTTTGAAAACGG+CGG + chr8_4:28658789-28658808 None:intergenic 45.0%
TTGGAGGTTAATGACCAAGC+TGG - chr8_4:28659479-28659498 Msa1322080:CDS 45.0%
TGGAGGTTAATGACCAAGCT+GGG - chr8_4:28659480-28659499 Msa1322080:CDS 45.0%
CATGGTCAGTCAGTTCTGTT+AGG - chr8_4:28659854-28659873 Msa1322080:CDS 45.0%
GCCATAATTACTGACCAGGA+TGG - chr8_4:28659965-28659984 Msa1322080:CDS 45.0%
CATTCTTCATTGCTCCATCC+TGG + chr8_4:28659982-28660001 None:intergenic 45.0%
TGTTTGATGAGCGTCATCGT+TGG - chr8_4:28660215-28660234 Msa1322080:CDS 45.0%
GTTTGATGAGCGTCATCGTT+GGG - chr8_4:28660216-28660235 Msa1322080:CDS 45.0%
ACGCTGCAACTCTTCATTTC+CGG + chr8_4:28660839-28660858 None:intergenic 45.0%
! AAAGCCTGCGATGCCTTTTA+CGG - chr8_4:28660865-28660884 Msa1322080:intron 45.0%
!! AAATCCGTAAAAGGCATCGC+AGG + chr8_4:28660872-28660891 None:intergenic 45.0%
CTGTAGCGACTCGAAGTAAA+GGG - chr8_4:28661043-28661062 Msa1322080:CDS 45.0%
!! GAAGCCTTCCTTTTTGAAGG+AGG + chr8_4:28661072-28661091 None:intergenic 45.0%
TTTGGGCAGATGCGAGATGT+AGG - chr8_4:28659732-28659751 Msa1322080:CDS 50.0%
TTGGGCAGATGCGAGATGTA+GGG - chr8_4:28659733-28659752 Msa1322080:CDS 50.0%
TATGCCACAAGCACCATCGA+TGG + chr8_4:28660033-28660052 None:intergenic 50.0%
! GTGAGAGACACATTTTGGGC+TGG - chr8_4:28660250-28660269 Msa1322080:CDS 50.0%
CCTTTACTTCGAGTCGCTAC+AGG + chr8_4:28661045-28661064 None:intergenic 50.0%
CCTGTAGCGACTCGAAGTAA+AGG - chr8_4:28661042-28661061 Msa1322080:CDS 50.0%
!! AAGGGCGTCCTCCTTCAAAA+AGG - chr8_4:28661061-28661080 Msa1322080:CDS 50.0%
GCGTCCTCCTTCAAAAAGGA+AGG - chr8_4:28661065-28661084 Msa1322080:CDS 50.0%
!! CGGCGGTGAATGTTGGTAAC+CGG + chr8_4:28658772-28658791 None:intergenic 55.0%
AAAGTGAGGCGGCTTCGACT+TGG - chr8_4:28659599-28659618 Msa1322080:CDS 55.0%
AAGTGAGGCGGCTTCGACTT+GGG - chr8_4:28659600-28659619 Msa1322080:CDS 55.0%
AGGTTGCGCCCTATTGTCGA+AGG - chr8_4:28659874-28659893 Msa1322080:CDS 55.0%
TTGCGCCCTATTGTCGAAGG+AGG - chr8_4:28659877-28659896 Msa1322080:CDS 55.0%
ATGGCGAGCTTTCGGGAAGA+CGG + chr8_4:28660014-28660033 None:intergenic 55.0%
ACCATCGATGGCGAGCTTTC+GGG + chr8_4:28660021-28660040 None:intergenic 55.0%
CACCATCGATGGCGAGCTTT+CGG + chr8_4:28660022-28660041 None:intergenic 55.0%
TCCCGAAAGCTCGCCATCGA+TGG - chr8_4:28660017-28660036 Msa1322080:CDS 60.0%
CTCGCCATCGATGGTGCTTG+TGG - chr8_4:28660026-28660045 Msa1322080:CDS 60.0%
!! AGGCGGCTTCGACTTGGGAC+AGG - chr8_4:28659605-28659624 Msa1322080:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 28658663 28661227 28658663 ID=Msa1322080;Name=Msa1322080
chr8_4 mRNA 28658663 28661227 28658663 ID=Msa1322080-mRNA-1;Parent=Msa1322080;Name=Msa1322080-mRNA-1;_AED=0.27;_eAED=0.37;_QI=0|0|0|0.66|0.5|0.33|3|0|802
chr8_4 exon 28660981 28661227 28660981 ID=Msa1322080-mRNA-1:exon:9579;Parent=Msa1322080-mRNA-1
chr8_4 exon 28660737 28660870 28660737 ID=Msa1322080-mRNA-1:exon:9578;Parent=Msa1322080-mRNA-1
chr8_4 exon 28658663 28660690 28658663 ID=Msa1322080-mRNA-1:exon:9577;Parent=Msa1322080-mRNA-1
chr8_4 CDS 28660981 28661227 28660981 ID=Msa1322080-mRNA-1:cds;Parent=Msa1322080-mRNA-1
chr8_4 CDS 28660737 28660870 28660737 ID=Msa1322080-mRNA-1:cds;Parent=Msa1322080-mRNA-1
chr8_4 CDS 28658663 28660690 28658663 ID=Msa1322080-mRNA-1:cds;Parent=Msa1322080-mRNA-1
Gene Sequence

>Msa1322080

ATGGAAGAGACAATCACGGCACACGTTTTGATTTTGGACAATCACAGTACAATCTTCCTTCTCCATAACCTTATATGTTCTGATTTTGTTCAACAACACAAACTCACCGGTTACCAACATTCACCGCCGTTTTCAAACTCGTTGTCGTCATTCTCTTATGCCTCTGTTTATCAGCATTGCTACTCTAACCTCAACCATCAATTGAAATTTGATTTCCTTATTTTAGCATCCAGTTCAAGAGACAATATGGAGAAAACTTTTAGATCTTTTGAAGAAGATGAAGAAGAGAGTGATCACATAAATGAAAATTGTGAGGATAATTTAACAGAAAATGAAGATGATGAACCTAAGGTTGGCATGAAATTTAATTCTACAGAGGAATTAGCACAATCAGTACAAAAAAGGGAGATGATGGAAAAAAAGTATTTTACTCTAGGGTGTAACCGTGCAAGAAGCTATGTGAGTAAGTCCAAAAATCTTTTAAAGCCAAATCCTAGCATGAAAACACACTGTAAGGCTAGAATAAATGTATGTGTGTCTTCAGATGGAACAATTACTATTTCTAAGGTTGCTCTTGAACATAATCATGACTTAATAAGTCCAAGCAAATCAAGATATTTTAGATGCAACAAGAATATAGACCCTAGTATAAAAAGGAGATTGGAGGTTAATGACCAAGCTGGGATTAATGTAAGTAGAAATTTTCGGTCTATGGTTGTTGAAGCAAATGGATATGATAACCTTACATTCGGGGAAAAAGATGTTAGAAACTACATTGACAAAGTGAGGCGGCTTCGACTTGGGACAGGAGACGCTGAAGCCATTCAAAATTATTTTGTTAGAATGCAAAGACAAAATAGTGAATTTTATTATGTAATGGATGTGGATGACAAAAGTCGTTTACGAAATGTTTTTTGGGCAGATGCGAGATGTAGGGCTGCTTATGAGTATTTTGGTGAAGTAATAACCTTCGACACCACTTACTTAACAAATAAATATGACATGCCTTTTGCTCCTTTTGTTGGAGTAAATCATCATGGTCAGTCAGTTCTGTTAGGTTGCGCCCTATTGTCGAAGGAGGATACTAAAACTTTTTCTTGGTTGTTCACTACTTGGTTAGAATGTATGCGTGGGCGTGCTCCACAAGCCATAATTACTGACCAGGATGGAGCAATGAAGAATGCAGTTGAGGCCGTCTTCCCGAAAGCTCGCCATCGATGGTGCTTGTGGCATATAATGAAAAAGGTTCCAGAAAAATTGAGTAGACTCTATGAGTCTATCAAAATACTTTTGCATGATGCGGTGTATGATTCTTTGAGCATAAGTGATTTCATGGAGAAGTGGGAAAAAATGATTGAATGTTATGAACTTCATGATAATGAATGGTTGAAAGGGTTGTTTGATGAGCGTCATCGTTGGGTTCCTGTGTATGTGAGAGACACATTTTGGGCTGGAATGTCAACAACACAACAAAGTGAAAGTATGAACGCATTTTTTGATGGATATGTGCACTCAAAGACATCATTGAAGCAATTTGTTGAACAATATGATAATGCATTGAAAGATAAAATTGAAAAGGAAAATATGGCTGATTTTAGTTCATCCAATAGAATTATTGCTTGTATAAGTCATTTTGGATTTGAATCTCAATTTCAAAAGGCATATACCAATGCAAAATTCAAAGAGTTTCAAGTAGAAGTGGCTTCTATGATGTACTGTCATGCTTTCTTTGAGAGAATGGAGGCTTTGAACTTAACATATTGTGTTACAGAAAGTAAAAAGGTATTTGATACAATTAAAGACATAATGTTCAAGGTATTCTTCAATGAGAAAGACTTTGAAATACGATGTACATGCTGTTTATTTGAGTTTAAGGGTATTTTATGTAGACATATCCTTTGTGTTCTTAAGCTCACAGCTAAAACAGAGTCAGTGCCCTCTTGTTATATATTGCCAAGATGGAGGAAGGATATTAAGCGAAGATATACACTTATTAAAAATGTTTTTGATCAATTAGCCGGAAATGAAGAGTTGCAGCGTGTAGCTAAAGCCTGCGATGCCTTTTACGGATTTGCTTCTTCATGTATCCATACTGAAAATGATTTATCAAAAGTAATGGATAGAATAGAGAACTTAAAACTGGAGATGACATGTCAAGAAAGTTTTTCAGAGATCACTGAAGGAGATAACTTAGCTCAAAATCAGATGACTAAAATTCTTGATCCTGTAGCGACTCGAAGTAAAGGGCGTCCTCCTTCAAAAAGGAAGGCTTCTAAAGTTGATCAAATTGTGAAAAAGAAGCTTGCTGGAAAGAAAGGACAAAAAAGCAACAAAAAAAGTAACACTTATCAAAGTCAAGAAGAGGTAATCTTGAAATTTTCAAGTTTATTTTATCTCCTTTACACGTAA
Protein sequence

>Msa1322080

MEETITAHVLILDNHSTIFLLHNLICSDFVQQHKLTGYQHSPPFSNSLSSFSYASVYQHCYSNLNHQLKFDFLILASSSRDNMEKTFRSFEEDEEESDHINENCEDNLTENEDDEPKVGMKFNSTEELAQSVQKREMMEKKYFTLGCNRARSYVSKSKNLLKPNPSMKTHCKARINVCVSSDGTITISKVALEHNHDLISPSKSRYFRCNKNIDPSIKRRLEVNDQAGINVSRNFRSMVVEANGYDNLTFGEKDVRNYIDKVRRLRLGTGDAEAIQNYFVRMQRQNSEFYYVMDVDDKSRLRNVFWADARCRAAYEYFGEVITFDTTYLTNKYDMPFAPFVGVNHHGQSVLLGCALLSKEDTKTFSWLFTTWLECMRGRAPQAIITDQDGAMKNAVEAVFPKARHRWCLWHIMKKVPEKLSRLYESIKILLHDAVYDSLSISDFMEKWEKMIECYELHDNEWLKGLFDERHRWVPVYVRDTFWAGMSTTQQSESMNAFFDGYVHSKTSLKQFVEQYDNALKDKIEKENMADFSSSNRIIACISHFGFESQFQKAYTNAKFKEFQVEVASMMYCHAFFERMEALNLTYCVTESKKVFDTIKDIMFKVFFNEKDFEIRCTCCLFEFKGILCRHILCVLKLTAKTESVPSCYILPRWRKDIKRRYTLIKNVFDQLAGNEELQRVAKACDAFYGFASSCIHTENDLSKVMDRIENLKLEMTCQESFSEITEGDNLAQNQMTKILDPVATRSKGRPPSKRKASKVDQIVKKKLAGKKGQKSNKKSNTYQSQEEVILKFSSLFYLLYT*