Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1322110 | sp|Q8GYA4|CRK10_ARATH | 43.210 | 648 | 333 | 12 | 28 | 652 | 34 | 669 | 1.22e-176 | 521 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1322110 | MtrunA17_Chr8g0354271 | 88.244 | 655 | 74 | 1 | 1 | 652 | 1 | 655 | 0.0 | 1153 |
| Msa1322110 | MtrunA17_Chr3g0081001 | 61.319 | 667 | 223 | 9 | 11 | 652 | 6 | 662 | 0.0 | 811 |
| Msa1322110 | MtrunA17_Chr3g0081011 | 61.801 | 644 | 225 | 8 | 25 | 652 | 20 | 658 | 0.0 | 810 |
| Msa1322110 | MtrunA17_Chr8g0354281 | 57.571 | 634 | 260 | 5 | 26 | 652 | 22 | 653 | 0.0 | 738 |
| Msa1322110 | MtrunA17_Chr8g0354421 | 55.007 | 669 | 280 | 7 | 1 | 652 | 18 | 682 | 0.0 | 736 |
| Msa1322110 | MtrunA17_Chr8g0354351 | 55.015 | 658 | 265 | 9 | 12 | 652 | 9 | 652 | 0.0 | 717 |
| Msa1322110 | MtrunA17_Chr8g0354441 | 53.949 | 671 | 288 | 6 | 1 | 652 | 1 | 669 | 0.0 | 713 |
| Msa1322110 | MtrunA17_Chr8g0354431 | 53.636 | 660 | 288 | 7 | 9 | 652 | 4 | 661 | 0.0 | 694 |
| Msa1322110 | MtrunA17_Chr3g0082941 | 62.475 | 501 | 155 | 7 | 167 | 652 | 1 | 483 | 0.0 | 617 |
| Msa1322110 | MtrunA17_Chr3g0081031 | 51.496 | 635 | 270 | 12 | 41 | 652 | 52 | 671 | 0.0 | 610 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1322110 | AT4G23180.1 | 43.210 | 648 | 333 | 12 | 28 | 652 | 34 | 669 | 1.24e-177 | 521 |
| Msa1322110 | AT4G05200.1 | 41.642 | 682 | 343 | 12 | 10 | 652 | 10 | 675 | 2.08e-170 | 502 |
| Msa1322110 | AT4G05200.2 | 41.642 | 682 | 343 | 12 | 10 | 652 | 28 | 693 | 3.70e-170 | 502 |
| Msa1322110 | AT4G23160.1 | 43.178 | 667 | 313 | 14 | 28 | 652 | 40 | 682 | 5.13e-167 | 494 |
| Msa1322110 | AT4G21410.3 | 42.313 | 683 | 342 | 18 | 11 | 652 | 8 | 679 | 1.21e-160 | 478 |
| Msa1322110 | AT4G23230.1 | 41.626 | 615 | 319 | 11 | 33 | 622 | 26 | 625 | 1.57e-158 | 470 |
| Msa1322110 | AT4G23140.1 | 42.900 | 669 | 320 | 17 | 28 | 652 | 24 | 674 | 1.60e-157 | 469 |
| Msa1322110 | AT4G23310.2 | 40.469 | 682 | 330 | 14 | 24 | 652 | 132 | 790 | 2.94e-155 | 467 |
| Msa1322110 | AT4G23310.2 | 29.675 | 246 | 155 | 6 | 28 | 263 | 23 | 260 | 3.03e-18 | 89.7 |
| Msa1322110 | AT4G38830.1 | 41.707 | 621 | 336 | 11 | 23 | 620 | 16 | 633 | 3.05e-155 | 463 |
Find 118 sgRNAs with CRISPR-Local
Find 212 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATCTAAATTAGGGGA+AGG | 0.594119 | 8_4:-28690921 | Msa1322110:CDS |
| AAAACTGATGTATTCAGCTT+TGG | 0.435845 | 8_4:-28689501 | Msa1322110:CDS |
| AAAAGAAATTCAGAAAAGTA+AGG | 0.510417 | 8_4:-28691866 | Msa1322110:CDS |
| AAAAGAGAAAGGAACTAAAT+TGG | 0.268939 | 8_4:-28689872 | Msa1322110:CDS |
| AAAAGGCTCTCAAGAACTTC+TGG | 0.330395 | 8_4:-28690757 | Msa1322110:CDS |
| AAATTAGGGGAAGGTGGTTT+TGG | 0.264935 | 8_4:-28690912 | Msa1322110:CDS |
| AACCCTAATTGGACTCATGT+TGG | 0.528762 | 8_4:-28691910 | Msa1322110:CDS |
| AACGTTGAATTGTGATCAAT+GGG | 0.429643 | 8_4:+28690961 | None:intergenic |
| AACGTTGAATTGTGATCAAT+GGG | 0.429643 | 8_4:+28691169 | None:intergenic |
| AACTACCCATATAATACAAT+TGG | 0.399945 | 8_4:+28691800 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAGAATTGCTAAGAACCTT+TGG | + | chr8_4:28688873-28688892 | None:intergenic | 30.0% | |
| ATAGTTATAACCTTTGCTTG+TGG | + | chr8_4:28688943-28688962 | None:intergenic | 30.0% | |
| GGTTATAACTATATCAGCAT+AGG | - | chr8_4:28688951-28688970 | Msa1322110:three_prime_UTR | 30.0% | |
| GAGCAACCAATTGTATTATA+TGG | - | chr8_4:28689274-28689293 | Msa1322110:CDS | 30.0% | |
| AGTAGAGATCTTACAAATGA+AGG | - | chr8_4:28689347-28689366 | Msa1322110:intron | 30.0% | |
| GTAGAGATCTTACAAATGAA+GGG | - | chr8_4:28689348-28689367 | Msa1322110:intron | 30.0% | |
| CTATTTACTACCAGTGAAAA+TGG | + | chr8_4:28689592-28689611 | None:intergenic | 30.0% | |
| AAGTCAAGAAACTTGATCAT+TGG | - | chr8_4:28689668-28689687 | Msa1322110:CDS | 30.0% | |
| AACGTTGAATTGTGATCAAT+GGG | + | chr8_4:28689914-28689933 | None:intergenic | 30.0% | |
| AACGTTGAATTGTGATCAAT+GGG | + | chr8_4:28689914-28689933 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 28688744 | 28692358 | 28688744 | ID=Msa1322110;Name=Msa1322110 |
| chr8_4 | mRNA | 28688744 | 28692358 | 28688744 | ID=Msa1322110-mRNA-1;Parent=Msa1322110;Name=Msa1322110-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0.66|0.71|1|0.83|0.71|7|282|652 |
| chr8_4 | exon | 28688744 | 28689337 | 28688744 | ID=Msa1322110-mRNA-1:exon:9608;Parent=Msa1322110-mRNA-1 |
| chr8_4 | exon | 28689419 | 28689569 | 28689419 | ID=Msa1322110-mRNA-1:exon:9607;Parent=Msa1322110-mRNA-1 |
| chr8_4 | exon | 28689663 | 28689900 | 28689663 | ID=Msa1322110-mRNA-1:exon:9606;Parent=Msa1322110-mRNA-1 |
| chr8_4 | exon | 28690602 | 28690812 | 28690602 | ID=Msa1322110-mRNA-1:exon:9605;Parent=Msa1322110-mRNA-1 |
| chr8_4 | exon | 28690899 | 28691035 | 28690899 | ID=Msa1322110-mRNA-1:exon:9604;Parent=Msa1322110-mRNA-1 |
| chr8_4 | exon | 28691127 | 28691243 | 28691127 | ID=Msa1322110-mRNA-1:exon:9603;Parent=Msa1322110-mRNA-1 |
| chr8_4 | exon | 28691566 | 28692358 | 28691566 | ID=Msa1322110-mRNA-1:exon:9602;Parent=Msa1322110-mRNA-1 |
| chr8_4 | CDS | 28691566 | 28692358 | 28691566 | ID=Msa1322110-mRNA-1:cds;Parent=Msa1322110-mRNA-1 |
| chr8_4 | CDS | 28691127 | 28691243 | 28691127 | ID=Msa1322110-mRNA-1:cds;Parent=Msa1322110-mRNA-1 |
| chr8_4 | CDS | 28690899 | 28691035 | 28690899 | ID=Msa1322110-mRNA-1:cds;Parent=Msa1322110-mRNA-1 |
| chr8_4 | CDS | 28690602 | 28690812 | 28690602 | ID=Msa1322110-mRNA-1:cds;Parent=Msa1322110-mRNA-1 |
| chr8_4 | CDS | 28689663 | 28689900 | 28689663 | ID=Msa1322110-mRNA-1:cds;Parent=Msa1322110-mRNA-1 |
| chr8_4 | CDS | 28689419 | 28689569 | 28689419 | ID=Msa1322110-mRNA-1:cds;Parent=Msa1322110-mRNA-1 |
| chr8_4 | CDS | 28689026 | 28689337 | 28689026 | ID=Msa1322110-mRNA-1:cds;Parent=Msa1322110-mRNA-1 |
| chr8_4 | three_prime_UTR | 28688744 | 28689025 | 28688744 | ID=Msa1322110-mRNA-1:three_prime_utr;Parent=Msa1322110-mRNA-1 |
| Gene Sequence |
>Msa1322110 ATGGAAGGAAGAAAACTAAAATTGACATCAATATTTTTTGCTTTCATACTATGTATAAGCTTCAAACAACTCTTTACAAAAGCTCAATCGCCCACTTTTGTGGGGGATGACTGTAATTACTCGACACCAAAGGTTCTTAGCAATTCTTACAAAACAAATCTCAAAAGCATCCTTTCATATCTATCTGCTGAAGCAGCCACAAGCAAAGGTTATAACTATATCAGCATAGGCGACAACGCAACAAAAGGAGATGCAGTGTATGGTCTCTACGATTGTCGTGGCGATGTAAGCTTTTGTGAATTCTGTGTCGCTTCTGCTACTAGAGAAGTTCTTGAGCGCTGCCCCAATAGAGTCGCCGCTACTATATTCTACAGCTACTGCATTTTGAGGTACTCAAATGAGAATTTCTTTGGGAAAGTCACAACAAACCCTAATTGGACTCATGTTGGATCCAAAACTGTCTCTAATGCAAAAGAAATTCAGAAAAGTAAGGATTTAATGAAATTTTTGATCGAAAAAGCAACTACTAGGAGCAACCAATTGTATTATATGGGTAGTTTCAATTTGAGTTCGAGTGAGGGTAGGTATGGACTGGTTCAATGCAGTAGAGATCTTACAAATGAAGGGTGCCGACAGTGTTTGGAGTTTATGTTAGCAAAGGTTGCCACATGTTGTGGACATAAGTTGGGGTGGCAGGTTTCATCTGCTAGTTGTCTAATTAGGTATGATGATTATATGTTCTATCAGAACCATGAGCAATCACCTTCGGTCCTTGTTCCTGATCCACAAACAGATTGGCTGGCACCTGCATATGAGGAAACATTGAATGCTGATCTTCCTACAATCCCATTGATCACAATTCAACGTTGTACTAATAACTGCTTCTGGTGCAAAAATAGAGTTAAAAAAGATTGGCTGGCACCTGCATATGAGGAAACATTGAATGCTGATCTTCCTACAATCCCATTGATCACAATTCAACGTTGTACTAATAATTTTTCAAAAACATCTAAATTAGGGGAAGGTGGTTTTGGATCTGTGTATAAGGGAATTCTTCCGGATGGAAGACAAATAGCAGTAAAAAGGCTCTCAAGAACTTCTGGTCAAGGCTCGGAGGAGTTCAAGAATGAAGTAATGTTTATAGCAAAATTGCAACATCGCAATCTTGTAAGACTTTTAGCATGCTGCTTGGAAGGAAATGAAAAGCTACTTGTGTATGAGTTTATGCCGAATGCAAGTCTCGACTTTCACCTGTTTGATAATGAAAAGAGAAAGGAACTAAATTGGAAACTAAGTTTGAGCATTATCAATGGAATAGCTAAAGGTCTTTTATACCTACATGAGGACTCTAGATTAAGAGTAATTCATAGAGATCTGAAAGCTAGCAATGTTCTATTAGATCATGAAATGAATCCCAAAATATCAGATTTTGGATTGGCAAGGGCATTTGACATAGGCCAGAACCAGGCAAATACAAGAAGAATAATGGGCACCTATGGATACATGGCACCAGAATACGCAATGGAAGGAGTATTTTCTGTGAAAACTGATGTATTCAGCTTTGGAGTATTAGTTTTGGAAATCATTAGCGGGAAAAAGAATACTGGATTCTATCTATCAGAACATGGTCAGAGTCTTCTTTTATATACATGGAAAAAATGGTGTGAAGGAACGTGTTTAGAAATAATGGATTCGGTACTAGAAAAATCCTGTATAGATGAACACGAAGTTGTGAGGTGCATAAACATTGGTTTATTGTGTGTTCAAGAAGATGCTGCAGATAGACCAACCATGTCAACTGTTGTTGTAATGTTGGCTAGTGACACAATGACCCTTCCAAAACCCAAACAACCTGCATTTTCAATCGGAAGAATGACCTCTAATGATTCATCTACCTCGAAAAGTTTCAAGGATCCTTCTATCAATGATGTAACAGTCTCAAATATTTTACCAAGGTAA |
| Protein sequence |
>Msa1322110 MEGRKLKLTSIFFAFILCISFKQLFTKAQSPTFVGDDCNYSTPKVLSNSYKTNLKSILSYLSAEAATSKGYNYISIGDNATKGDAVYGLYDCRGDVSFCEFCVASATREVLERCPNRVAATIFYSYCILRYSNENFFGKVTTNPNWTHVGSKTVSNAKEIQKSKDLMKFLIEKATTRSNQLYYMGSFNLSSSEGRYGLVQCSRDLTNEGCRQCLEFMLAKVATCCGHKLGWQVSSASCLIRYDDYMFYQNHEQSPSVLVPDPQTDWLAPAYEETLNADLPTIPLITIQRCTNNCFWCKNRVKKDWLAPAYEETLNADLPTIPLITIQRCTNNFSKTSKLGEGGFGSVYKGILPDGRQIAVKRLSRTSGQGSEEFKNEVMFIAKLQHRNLVRLLACCLEGNEKLLVYEFMPNASLDFHLFDNEKRKELNWKLSLSIINGIAKGLLYLHEDSRLRVIHRDLKASNVLLDHEMNPKISDFGLARAFDIGQNQANTRRIMGTYGYMAPEYAMEGVFSVKTDVFSFGVLVLEIISGKKNTGFYLSEHGQSLLLYTWKKWCEGTCLEIMDSVLEKSCIDEHEVVRCINIGLLCVQEDAADRPTMSTVVVMLASDTMTLPKPKQPAFSIGRMTSNDSSTSKSFKDPSINDVTVSNILPR* |
