AlfalfaGEDB | Msa1322120

M. sativa cultivar zhongmu-4 / Msa1322120

KEGG: -
GO: -
PFAM: PF01657.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1322120	sp\|O81906\|B120_ARATH	63.500	200	73	0	779	978	539	738	2.45e-70	254
Msa1322120	sp\|O81906\|B120_ARATH	34.667	375	196	14	526	874	376	727	5.61e-38	157
Msa1322120	sp\|O81906\|B120_ARATH	72.222	72	20	0	317	388	546	617	3.34e-24	113

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1322120	Q1S5M3	58.738	412	134	4	12	387	19	430	6.54e-160	495
Msa1322120	Q1S5M3	62.455	277	90	3	723	985	283	559	9.09e-96	327
Msa1322120	Q1S5M3	97.820	367	7	1	388	753	64	430	0.0	748

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1322120	MtrunA17_Chr8g0354351	85.096	416	37	1	1	391	10	425	0.0	737
Msa1322120	MtrunA17_Chr8g0354351	65.041	369	118	3	388	755	65	423	2.89e-170	512
Msa1322120	MtrunA17_Chr8g0354351	64.729	258	79	3	737	985	296	550	7.32e-100	328
Msa1322120	MtrunA17_Chr8g0354441	59.722	432	131	7	1	391	13	442	4.57e-169	510
Msa1322120	MtrunA17_Chr8g0354441	64.247	372	125	4	390	755	71	440	2.10e-161	490
Msa1322120	MtrunA17_Chr8g0354441	70.085	234	61	1	761	985	334	567	9.46e-99	325
Msa1322120	MtrunA17_Chr8g0354281	59.564	413	129	6	12	391	20	427	1.09e-161	490
Msa1322120	MtrunA17_Chr8g0354281	62.133	375	119	8	389	755	66	425	1.46e-152	466
Msa1322120	MtrunA17_Chr8g0354281	67.870	277	67	4	723	985	284	552	3.14e-108	350
Msa1322120	MtrunA17_Chr8g0354421	58.353	425	134	8	4	391	37	455	1.28e-160	488

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1322120	AT4G21390.2	62.679	209	77	1	779	987	539	746	1.07e-72	256
Msa1322120	AT4G21390.2	34.015	391	209	14	526	890	376	743	3.48e-39	157
Msa1322120	AT4G21390.2	72.222	72	20	0	317	388	546	617	3.03e-25	113
Msa1322120	AT4G21390.1	63.500	200	73	0	779	978	539	738	2.49e-71	254
Msa1322120	AT4G21390.1	34.667	375	196	14	526	874	376	727	5.70e-39	157
Msa1322120	AT4G21390.1	72.222	72	20	0	317	388	546	617	3.40e-25	113
Msa1322120	AT4G23130.3	52.083	240	109	3	752	990	332	566	1.33e-70	246
Msa1322120	AT4G23130.3	36.041	394	224	10	394	770	65	447	1.77e-53	197
Msa1322120	AT4G23130.3	33.810	420	226	13	10	386	20	430	5.94e-52	192
Msa1322120	AT4G23180.2	55.024	209	94	0	779	987	358	566	1.72e-70	246

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Find 194 sgRNAs with CRISPR-Local

Find 831 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCTTTCAGAAACTTC+CGG	0.497774	8_4:-28698314	Msa1322120:CDS
AAAGAACGTTGCAGGCTTTA+AGG	0.185267	8_4:+28694921	None:intergenic
AAAGGTTGTTAACATTGGAT+TGG	0.458643	8_4:+28707875	None:intergenic
AAATTAGGACAAGGTGGATT+TGG	0.300019	8_4:-28698487	Msa1322120:CDS
AACAACCCAAGAGTTTCACA+AGG	0.654960	8_4:+28698231	None:intergenic
AACAATGATGATGTTGTTTA+TGG	0.212550	8_4:-28707793	Msa1322120:CDS
AACACCGGTTCAAGGTTCAG+AGG	0.660334	8_4:-28695527	Msa1322120:CDS
AACATCATCATTGTTTCCTA+AGG	0.492540	8_4:+28707801	None:intergenic
AACGACATAAGCAACTAGAT+TGG	0.486866	8_4:-28695030	Msa1322120:CDS
AACTCCAAAGCTGAAAACAT+CGG	0.629248	8_4:+28694650	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGTTGTTAACATTGGAT+TGG	+	chr8_4:28694667-28694686	None:intergenic	30.0%
AACATCATCATTGTTTCCTA+AGG	+	chr8_4:28694741-28694760	None:intergenic	30.0%
TACTCGAACCAAAATTACTT+TGG	-	chr8_4:28694902-28694921	Msa1322120:CDS	30.0%
TATCAGTTAACTGAGAATAC+AGG	-	chr8_4:28695250-28695269	Msa1322120:intron	30.0%
ATTATGAATAAATACCGGTC+TGG	-	chr8_4:28695358-28695377	Msa1322120:intron	30.0%
AACAACTTCAACTGTATTTC+CGG	+	chr8_4:28695826-28695845	None:intergenic	30.0%
AATACAGTTGAAGTTGTTAG+TGG	-	chr8_4:28695828-28695847	Msa1322120:intron	30.0%
CCAAATGTGTGTTAAAGAAT+AGG	+	chr8_4:28695884-28695903	None:intergenic	30.0%
CCTATTCTTTAACACACATT+TGG	-	chr8_4:28695881-28695900	Msa1322120:intron	30.0%
TGACAACTGCTCTATAAATT+AGG	-	chr8_4:28696032-28696051	Msa1322120:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	28694554	28708007	28694554	ID=Msa1322120;Name=Msa1322120
chr8_4	mRNA	28694554	28708007	28694554	ID=Msa1322120-mRNA-1;Parent=Msa1322120;Name=Msa1322120-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0\|0.41\|0.30\|1\|0.66\|0.69\|13\|0\|990
chr8_4	exon	28707263	28708007	28707263	ID=Msa1322120-mRNA-1:exon:9601;Parent=Msa1322120-mRNA-1
chr8_4	exon	28706360	28706467	28706360	ID=Msa1322120-mRNA-1:exon:9600;Parent=Msa1322120-mRNA-1
chr8_4	exon	28706186	28706280	28706186	ID=Msa1322120-mRNA-1:exon:9599;Parent=Msa1322120-mRNA-1
chr8_4	exon	28705655	28705865	28705655	ID=Msa1322120-mRNA-1:exon:9598;Parent=Msa1322120-mRNA-1
chr8_4	exon	28699767	28700360	28699767	ID=Msa1322120-mRNA-1:exon:9597;Parent=Msa1322120-mRNA-1
chr8_4	exon	28698730	28698852	28698730	ID=Msa1322120-mRNA-1:exon:9596;Parent=Msa1322120-mRNA-1
chr8_4	exon	28698474	28698643	28698474	ID=Msa1322120-mRNA-1:exon:9595;Parent=Msa1322120-mRNA-1
chr8_4	exon	28698159	28698369	28698159	ID=Msa1322120-mRNA-1:exon:9594;Parent=Msa1322120-mRNA-1
chr8_4	exon	28696668	28696681	28696668	ID=Msa1322120-mRNA-1:exon:9593;Parent=Msa1322120-mRNA-1
chr8_4	exon	28696502	28696564	28696502	ID=Msa1322120-mRNA-1:exon:9592;Parent=Msa1322120-mRNA-1
chr8_4	exon	28695386	28695617	28695386	ID=Msa1322120-mRNA-1:exon:9591;Parent=Msa1322120-mRNA-1
chr8_4	exon	28694821	28695058	28694821	ID=Msa1322120-mRNA-1:exon:9590;Parent=Msa1322120-mRNA-1
chr8_4	exon	28694554	28694722	28694554	ID=Msa1322120-mRNA-1:exon:9589;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28707263	28708007	28707263	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28706360	28706467	28706360	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28706186	28706280	28706186	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28705655	28705865	28705655	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28699767	28700360	28699767	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28698730	28698852	28698730	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28698474	28698643	28698474	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28698159	28698369	28698159	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28696668	28696681	28696668	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28696502	28696564	28696502	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28695386	28695617	28695386	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28694821	28695058	28694821	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1
chr8_4	CDS	28694554	28694722	28694554	ID=Msa1322120-mRNA-1:cds;Parent=Msa1322120-mRNA-1

Gene Sequence

>Msa1322120

ATGGCCATGTTGTTTCTCCTCCTTGTCCTCATCACCTCAACTAGTGCACAAACACCAATTTACATGTACAATTTTTGCAACTCAACACAAAAATCTCTAAGCACTTCATACCAATCCAATGTTAACAACCTTTTCTTATGGTTCAACTCTGATTCTGCCACTGGAAAAGAATCTAACCACACCACCTTAGGAAACAATGATGATGTTGTTTATGGAAGCTATGATTGTCGTGGTGACATCGCGGATCAATTCTGTCGATTCTGTATCAATAGTGCTATCAACGAAATTGCTCAACGTTGTCCTAATGGTGTGTCAGCTGTAATATGGTATGATATATGCATCATTCGTTACTCGAACCAAAATTACTTTGGAAAAGTTGTCGTAACACCATCATGGAATATTACTGGATCCAAAAGCATAAAGGACTCAACAGAACTTGGGAAAGCAGTAGATTTGATGAGGAGTTTGATGGGAAATATGATAGAAGCAAACCGATCATGGGCAACGAGTGAGTTCAATTGGAGTGATACCGAGAAAAGATATGGTTTGGTTCAGTGTTCTGGAGACCTTAATAGTGATGGGTGCAGACAGTGTTTGGAGACTTTGTTAGACAAAATTCGAGTGTGTTGTGGGACTAAGGTAACATGGGCTGTTGTAGCTCCAAGTTGTGGGATTAGATTTGATGATCAAAAGTTCTATCAGTTAACTGAGAATACAGGATCTTCTTCTCCTGTGCCAAATCCAGCCAAGCATGAAGGAGCAAGTAAGAACAAAACCTTGGTCATCACCTTATCAAGTGTACTTGTGGCAGTAGCTTTAGTATGTTTTTGTGTCTTCATGAGATTAAGAAAAGGTGGATCGATATTCAAGATCATTCCTAAAGCAGTTCATAATCATGTTCAAAGAGATGATTCATTGGATGGAGACCTTCCAATAATCCCTTTAACAGGTACTTTACCAGACGGGACAGAAATTGCGGTAAAAAGGCTTGCAGAAGCATCTAATCAAGGTTTAGAGGAGTTCAAGAATGAAGTAATATTCATAGCTAAATTGCAACACAGGAACCTTGTGAAGCTATTAGGTTGCTGCATTGAAGAAAATGAAAAGATATTAGTATATGAGTACATGCCGAATTCAAGTCTTGACTTTCATCTTTTCAGCCAAGAATCTAACCTAACCTCCATAGGAAGCAACAAGAATGATCACAATGACACAGTTTACGGAAACTATGACTGTCGAGGCGACATACCTGCACACTTCTGTAATTTCTGTGTCAACAGTGCTGTCAAAGAAATTTCACAAACTTGTACAAATGGTACCTCAGCTATTATATTCTATGACATGTGCATATTACGTTACTCTAACAAGAATTTCTTTGGAAAAATCAACTTATCTCCTTCATTTAATTTAACTGGACCAAAAACAATAAAGGACTCCAGGGATTTCGCGAAAGCCGAGGGTTTGATGAAGGATTTGATAAGGAAGACGACGGATGCTAATAGAATGTGGGCGACAAGCGTGTTTGATTGGAGTGATACCGAGAAAAGATATGGTTTGGTTCAGTGTGCCGGAGATCTTGATAATAATGGATGTAAAGAGTGTTTGAAGGAGTTGATAGATAGGGTTGCTGAGTGTTGTGGAACATCAGTAATGTGGGGTATTGTTGCTCCAAGTTGTGGAGTTAGGTTTGATGATAAAATGTTTTTTCAGGTATCTTCTGAGCAACCAGGGTCCTCTTCTTTGCCAAATCCAGCCAAGAAGGAAGGTTCAAGTAATAAAAAAACCTTGACCATCAGCTTAGTAAGTGTACTAATGGCAGTAGCTCTATTAACTTGTGTCTACTACTCATGGAGAAGGAATAATAGGTTAAGTCAAGGTGAATCGACGTTGAGCACCACTCCTCTAGCTTTTCATGGTCATGTTCTAAGAGATGATTCATTGAATGGAGACCTTCCAATAATCCCTTTAATTGTACTTCAACAAAGCACTGATTACTTTTCAGAGTCAACTAAATTAGGACAAGGTGGATTTGGATCTGTTTACAAGGGTACATTACCAGATGGGACTGAAATTGCGGCCAAAAGGCTTTCAGAAACTTCCGGTCAAGGTTTAGAGGAGTTCAAGAATGAAGTGATTTTCATAGCAAAACTACAACATAGAAACCTTGTGAAACTCTTGGGTTGTTGCTTTGAACAAAATGAAAAGATACTAGTGTACGAGTACATGCCAAATTCAAGCCTTGATTTTCACCTTTTTAACTTGGAAACTATGGAAGATGCTGCAGATCGACCAACCATGTCCTCCATTGTTCTCATGCTAGGGAGCGACACGATGGGATTTTTTTTGAATTTTTCAAACATGGTACTATCGGGTGAATATAAGATCGTCAAAAGGCTTGCAGAAACACCGGTTCAAGGTTCAGAGGAGTTCAAGAATGAAGTAATCTTCATAGCTAAACTGCAACATAGAAATCTTGTGAAACTCTTAGGTTGCTGCATTGAAGACAATGAAAAGATACTAGTATATGAGTACATGCCAAATTCAAGCCCTGACTTTCACCTTTTCAATGAAGAACGACATAAGCAACTAGATTGGACATTATTACTAAGTATTATCAATGGAATTGCAAGAGAACTTTTATACCTTCACCAGGATTCTCGACTTACAGGTATCCGTAGAGACCTTAAAGCCTGCAACGTTCTTTTGGATGATGAAATGAATCCAAAAATATCAGATTTGGGATTGGCAAGGAAATTTGAAAAAGGTCAAAGTCATAGAAAAACAAAAAGAGTTATTGGCACCTACGGATACATGGCTCCAGAATATGCTATGGTTGGATTGTATTCAGTGAAATCCGATGTTTTCAGCTTTGGAGTTCTTTTATTAGAAGTTGTTTATGGAAAAAGGAATGGTGAATTTGTTCTTTCAGAACATAGGCAAAGTCTTCTATTATATATTTCATCAACAAACTGA

Protein sequence

>Msa1322120

MAMLFLLLVLITSTSAQTPIYMYNFCNSTQKSLSTSYQSNVNNLFLWFNSDSATGKESNHTTLGNNDDVVYGSYDCRGDIADQFCRFCINSAINEIAQRCPNGVSAVIWYDICIIRYSNQNYFGKVVVTPSWNITGSKSIKDSTELGKAVDLMRSLMGNMIEANRSWATSEFNWSDTEKRYGLVQCSGDLNSDGCRQCLETLLDKIRVCCGTKVTWAVVAPSCGIRFDDQKFYQLTENTGSSSPVPNPAKHEGASKNKTLVITLSSVLVAVALVCFCVFMRLRKGGSIFKIIPKAVHNHVQRDDSLDGDLPIIPLTGTLPDGTEIAVKRLAEASNQGLEEFKNEVIFIAKLQHRNLVKLLGCCIEENEKILVYEYMPNSSLDFHLFSQESNLTSIGSNKNDHNDTVYGNYDCRGDIPAHFCNFCVNSAVKEISQTCTNGTSAIIFYDMCILRYSNKNFFGKINLSPSFNLTGPKTIKDSRDFAKAEGLMKDLIRKTTDANRMWATSVFDWSDTEKRYGLVQCAGDLDNNGCKECLKELIDRVAECCGTSVMWGIVAPSCGVRFDDKMFFQVSSEQPGSSSLPNPAKKEGSSNKKTLTISLVSVLMAVALLTCVYYSWRRNNRLSQGESTLSTTPLAFHGHVLRDDSLNGDLPIIPLIVLQQSTDYFSESTKLGQGGFGSVYKGTLPDGTEIAAKRLSETSGQGLEEFKNEVIFIAKLQHRNLVKLLGCCFEQNEKILVYEYMPNSSLDFHLFNLETMEDAADRPTMSSIVLMLGSDTMGFFLNFSNMVLSGEYKIVKRLAETPVQGSEEFKNEVIFIAKLQHRNLVKLLGCCIEDNEKILVYEYMPNSSPDFHLFNEERHKQLDWTLLLSIINGIARELLYLHQDSRLTGIRRDLKACNVLLDDEMNPKISDLGLARKFEKGQSHRKTKRVIGTYGYMAPEYAMVGLYSVKSDVFSFGVLLLEVVYGKRNGEFVLSEHRQSLLLYISSTN*