AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1323240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323240 MtrunA17_Chr1g0207551 36.364 165 81 2 78 218 33 197 4.35e-26 105
Msa1323240 MtrunA17_Chr2g0329031 33.523 176 86 4 67 218 2 170 7.23e-22 93.2
Msa1323240 MtrunA17_Chr5g0448761 30.233 172 89 4 78 218 59 230 3.39e-18 82.8
Msa1323240 MtrunA17_Chr3g0080351 28.333 180 67 4 78 218 75 231 3.61e-17 79.7
Msa1323240 MtrunA17_Chr6g0451441 28.500 200 121 5 40 218 49 247 3.58e-14 70.9
Msa1323240 MtrunA17_Chr2g0287651 24.468 188 112 4 58 215 53 240 1.04e-13 69.7
Msa1323240 MtrunA17_Chr7g0258861 26.220 164 94 3 78 214 9 172 1.98e-12 65.1
Msa1323240 MtrunA17_Chr5g0440451 28.571 133 76 3 39 154 1 131 7.19e-12 64.3
Msa1323240 MtrunA17_Chr7g0233911 33.793 145 62 6 49 162 2 143 1.97e-11 61.2
Msa1323240 MtrunA17_Chr2g0324011 26.627 169 94 5 78 218 9 175 2.43e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323240 AT4G15090.1 35.023 217 104 6 39 218 1 217 8.49e-29 114
Msa1323240 AT4G15090.5 35.023 217 104 6 39 218 1 217 8.49e-29 114
Msa1323240 AT4G15090.3 35.023 217 104 6 39 218 1 217 8.49e-29 114
Msa1323240 AT4G15090.2 35.023 217 104 6 39 218 1 217 8.49e-29 114
Msa1323240 AT4G15090.4 35.023 217 104 6 39 218 1 217 8.49e-29 114
Msa1323240 AT4G38180.1 31.148 183 94 4 66 218 62 242 2.16e-21 92.4
Msa1323240 AT3G22170.2 27.273 242 101 7 30 218 7 226 1.13e-19 87.4
Msa1323240 AT3G22170.1 27.273 242 101 7 30 218 7 226 1.13e-19 87.4
Msa1323240 AT1G76320.2 31.875 160 79 4 83 218 1 154 1.16e-16 78.6
Msa1323240 AT1G76320.1 31.875 160 79 4 83 218 1 154 1.21e-16 78.6
Msa1323240 AT1G76320.4 31.875 160 79 4 83 218 1 154 1.21e-16 78.6
Msa1323240 AT1G76320.3 31.875 160 79 4 83 218 1 154 1.70e-16 78.2
Msa1323240 AT2G32250.5 37.500 112 45 3 82 170 41 150 6.23e-14 70.5
Msa1323240 AT2G32250.2 37.500 112 45 3 82 170 41 150 6.61e-14 70.5
Msa1323240 AT2G32250.4 37.500 112 45 3 82 170 41 150 6.61e-14 70.5
Msa1323240 AT2G32250.6 37.500 112 45 3 82 170 41 150 6.80e-14 70.5
Msa1323240 AT2G32250.1 37.500 112 45 3 82 170 41 150 6.80e-14 70.5
Msa1323240 AT2G32250.3 37.500 112 45 3 82 170 41 150 6.80e-14 70.5
Msa1323240 AT2G32250.8 36.937 111 45 3 83 170 1 109 5.13e-13 67.8
Msa1323240 AT2G32250.7 36.937 111 45 3 83 170 1 109 5.23e-13 67.8

Find 38 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGAAATATAGGAATTAA+TGG 0.248279 8_4:-31442452 Msa1323240:intron
CACTCTGCTATCATTCTTAA+AGG 0.277937 8_4:-31442720 Msa1323240:CDS
TTCATATCCTCCATATTGTT+TGG 0.293868 8_4:+31441904 None:intergenic
AAATCTGCGTCGTTGTTTAA+AGG 0.297168 8_4:-31442134 Msa1323240:CDS
GCCACTCTGGCCAAACAATA+TGG 0.314518 8_4:-31441914 Msa1323240:CDS
TCAAATTTCATTCCAATGTA+TGG 0.346422 8_4:+31442322 None:intergenic
GAGTAAAGTGTAATGAAAAT+TGG 0.367197 8_4:-31442822 None:intergenic
ATCAGAAATCATTTAGATAT+AGG 0.417224 8_4:-31441857 Msa1323240:CDS
CATTATTTCCTTTGCCATAG+AGG 0.426978 8_4:-31442007 Msa1323240:CDS
GAATTAATGGAAGTTGTTGA+TGG 0.448629 8_4:-31442439 Msa1323240:CDS
AATGATGACAATGTTATTGA+TGG 0.455364 8_4:-31442415 Msa1323240:CDS
AGGAAATAATGTGCATATGC+TGG 0.467902 8_4:+31442019 None:intergenic
AGCGTGTGTTGAAGGGAGTT+TGG 0.475218 8_4:+31442782 None:intergenic
TGCTATCATTCTTAAAGGTA+TGG 0.477048 8_4:-31442715 Msa1323240:intron
TGTGTGTGAGCGTGTGTTGA+AGG 0.481794 8_4:+31442774 None:intergenic
ACTTATGCTCGCACAAGATA+CGG 0.484780 8_4:-31442181 Msa1323240:CDS
AGAATGATTCATGTACGGTT+GGG 0.505397 8_4:-31442360 Msa1323240:CDS
GTGTGTGAGCGTGTGTTGAA+GGG 0.519552 8_4:+31442775 None:intergenic
TATTGATTCCTCTATGGCAA+AGG 0.524300 8_4:+31441999 None:intergenic
ACTACTGTAACATAAATCTT+CGG 0.535461 8_4:+31442744 None:intergenic
CTTATGCTCGCACAAGATAC+GGG 0.542745 8_4:-31442180 Msa1323240:CDS
AATTAATGGAAGTTGTTGAT+GGG 0.547188 8_4:-31442438 Msa1323240:CDS
ATTGAAACTATGCAAGATGT+TGG 0.548761 8_4:-31441959 Msa1323240:CDS
TCCATATTGTTTGGCCAGAG+TGG 0.548986 8_4:+31441913 None:intergenic
GAGAATGATTCATGTACGGT+TGG 0.560679 8_4:-31442361 Msa1323240:CDS
CTAATGAAAGAGCCATACAT+TGG 0.562140 8_4:-31442334 Msa1323240:CDS
AAGCAAAATTTATGCCACTC+TGG 0.565072 8_4:-31441927 Msa1323240:CDS
TCTGCGTCGTTGTTTAAAGG+TGG 0.568390 8_4:-31442131 Msa1323240:CDS
TTGTTTAAAGGTGGAATGTG+AGG 0.581351 8_4:-31442122 Msa1323240:CDS
TATGGAGGATATGAAAACAT+TGG 0.602190 8_4:-31441896 Msa1323240:CDS
TGTTGAGAATGATTCATGTA+CGG 0.602674 8_4:-31442365 Msa1323240:CDS
ATGATTCATGTACGGTTGGG+AGG 0.609038 8_4:-31442357 Msa1323240:CDS
GAAATCATTTAGATATAGGT+CGG 0.612130 8_4:-31441853 Msa1323240:CDS
GCACAAGATACGGGAAGACG+CGG 0.628660 8_4:-31442171 Msa1323240:CDS
ATGATGACAATGTTATTGAT+GGG 0.630031 8_4:-31442414 Msa1323240:CDS
ACTGTAACATAAATCTTCGG+TGG 0.635445 8_4:+31442747 None:intergenic
CACAAGATACGGGAAGACGC+GGG 0.645645 8_4:-31442170 Msa1323240:CDS
ACTCTGGCCAAACAATATGG+AGG 0.695583 8_4:-31441911 Msa1323240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGGTAAATATTACTATAAAA+TGG - chr8_4:31441962-31441981 Msa1323240:CDS 15.0%
!!! CTTATTTTTTTAGAAAAAGT+TGG + chr8_4:31442106-31442125 None:intergenic 15.0%
!!! GTTTTAATCTTTATTTTACT+CGG - chr8_4:31442125-31442144 Msa1323240:CDS 15.0%
!!! ACAGTTATAATTTGAAATAT+AGG - chr8_4:31442196-31442215 Msa1323240:intron 15.0%
!!! ATTTGAAATATAGGAATTAA+TGG - chr8_4:31442205-31442224 Msa1323240:intron 15.0%
!! TGGTAAATATTACTATAAAA+TGG - chr8_4:31441962-31441981 Msa1323240:CDS 15.0%
!!! CTTATTTTTTTAGAAAAAGT+TGG + chr8_4:31442106-31442125 None:intergenic 15.0%
!!! GTTTTAATCTTTATTTTACT+CGG - chr8_4:31442125-31442144 Msa1323240:CDS 15.0%
!!! ACAGTTATAATTTGAAATAT+AGG - chr8_4:31442196-31442215 Msa1323240:intron 15.0%
!!! ATTTGAAATATAGGAATTAA+TGG - chr8_4:31442205-31442224 Msa1323240:intron 15.0%
!! TAAAATGGAATAGAATTTGA+TGG - chr8_4:31441977-31441996 Msa1323240:CDS 20.0%
!! TAATATAAGCACTATATATG+TGG - chr8_4:31442019-31442038 Msa1323240:CDS 20.0%
!! CGTATTAAAAAAAATTGTGA+TGG - chr8_4:31442566-31442585 Msa1323240:intron 20.0%
!!! AAAAAAATTGTGATGGAAAA+TGG - chr8_4:31442573-31442592 Msa1323240:intron 20.0%
!! ATCAGAAATCATTTAGATAT+AGG - chr8_4:31442800-31442819 Msa1323240:CDS 20.0%
!! TAAAATGGAATAGAATTTGA+TGG - chr8_4:31441977-31441996 Msa1323240:CDS 20.0%
!! TAATATAAGCACTATATATG+TGG - chr8_4:31442019-31442038 Msa1323240:CDS 20.0%
!! CGTATTAAAAAAAATTGTGA+TGG - chr8_4:31442566-31442585 Msa1323240:intron 20.0%
!!! AAAAAAATTGTGATGGAAAA+TGG - chr8_4:31442573-31442592 Msa1323240:intron 20.0%
!! ATCAGAAATCATTTAGATAT+AGG - chr8_4:31442800-31442819 Msa1323240:CDS 20.0%
! ACTACTGTAACATAAATCTT+CGG + chr8_4:31441916-31441935 None:intergenic 25.0%
! AATTAATGGAAGTTGTTGAT+GGG - chr8_4:31442219-31442238 Msa1323240:intron 25.0%
! AATGATGACAATGTTATTGA+TGG - chr8_4:31442242-31442261 Msa1323240:intron 25.0%
! ATGATGACAATGTTATTGAT+GGG - chr8_4:31442243-31442262 Msa1323240:intron 25.0%
! TCAAATTTCATTCCAATGTA+TGG + chr8_4:31442338-31442357 None:intergenic 25.0%
! GAAATCATTTAGATATAGGT+CGG - chr8_4:31442804-31442823 Msa1323240:CDS 25.0%
! ACTACTGTAACATAAATCTT+CGG + chr8_4:31441916-31441935 None:intergenic 25.0%
! AATTAATGGAAGTTGTTGAT+GGG - chr8_4:31442219-31442238 Msa1323240:intron 25.0%
! AATGATGACAATGTTATTGA+TGG - chr8_4:31442242-31442261 Msa1323240:intron 25.0%
! ATGATGACAATGTTATTGAT+GGG - chr8_4:31442243-31442262 Msa1323240:intron 25.0%
! TCAAATTTCATTCCAATGTA+TGG + chr8_4:31442338-31442357 None:intergenic 25.0%
! GAAATCATTTAGATATAGGT+CGG - chr8_4:31442804-31442823 Msa1323240:CDS 25.0%
TGCTATCATTCTTAAAGGTA+TGG - chr8_4:31441942-31441961 Msa1323240:CDS 30.0%
GAATTAATGGAAGTTGTTGA+TGG - chr8_4:31442218-31442237 Msa1323240:intron 30.0%
TGTTGAGAATGATTCATGTA+CGG - chr8_4:31442292-31442311 Msa1323240:CDS 30.0%
TAAAATTAGTCGTCGTTCAA+AGG - chr8_4:31442422-31442441 Msa1323240:CDS 30.0%
!!! GAGTTTTATTGATTCCTCTA+TGG + chr8_4:31442667-31442686 None:intergenic 30.0%
ATTGAAACTATGCAAGATGT+TGG - chr8_4:31442698-31442717 Msa1323240:intron 30.0%
TTCATATCCTCCATATTGTT+TGG + chr8_4:31442756-31442775 None:intergenic 30.0%
TATGGAGGATATGAAAACAT+TGG - chr8_4:31442761-31442780 Msa1323240:CDS 30.0%
TGCTATCATTCTTAAAGGTA+TGG - chr8_4:31441942-31441961 Msa1323240:CDS 30.0%
GAATTAATGGAAGTTGTTGA+TGG - chr8_4:31442218-31442237 Msa1323240:intron 30.0%
TGTTGAGAATGATTCATGTA+CGG - chr8_4:31442292-31442311 Msa1323240:CDS 30.0%
TAAAATTAGTCGTCGTTCAA+AGG - chr8_4:31442422-31442441 Msa1323240:CDS 30.0%
!!! GAGTTTTATTGATTCCTCTA+TGG + chr8_4:31442667-31442686 None:intergenic 30.0%
ATTGAAACTATGCAAGATGT+TGG - chr8_4:31442698-31442717 Msa1323240:intron 30.0%
TTCATATCCTCCATATTGTT+TGG + chr8_4:31442756-31442775 None:intergenic 30.0%
TATGGAGGATATGAAAACAT+TGG - chr8_4:31442761-31442780 Msa1323240:CDS 30.0%
ACTGTAACATAAATCTTCGG+TGG + chr8_4:31441913-31441932 None:intergenic 35.0%
CACTCTGCTATCATTCTTAA+AGG - chr8_4:31441937-31441956 Msa1323240:CDS 35.0%
AGAATGATTCATGTACGGTT+GGG - chr8_4:31442297-31442316 Msa1323240:CDS 35.0%
CTAATGAAAGAGCCATACAT+TGG - chr8_4:31442323-31442342 Msa1323240:CDS 35.0%
AAATCTGCGTCGTTGTTTAA+AGG - chr8_4:31442523-31442542 Msa1323240:intron 35.0%
TTGTTTAAAGGTGGAATGTG+AGG - chr8_4:31442535-31442554 Msa1323240:intron 35.0%
CTGGAAAAAGCTCATGATTA+TGG + chr8_4:31442622-31442641 None:intergenic 35.0%
AGGAAATAATGTGCATATGC+TGG + chr8_4:31442641-31442660 None:intergenic 35.0%
CATTATTTCCTTTGCCATAG+AGG - chr8_4:31442650-31442669 Msa1323240:intron 35.0%
!! TATTGATTCCTCTATGGCAA+AGG + chr8_4:31442661-31442680 None:intergenic 35.0%
AAGCAAAATTTATGCCACTC+TGG - chr8_4:31442730-31442749 Msa1323240:CDS 35.0%
ACTGTAACATAAATCTTCGG+TGG + chr8_4:31441913-31441932 None:intergenic 35.0%
CACTCTGCTATCATTCTTAA+AGG - chr8_4:31441937-31441956 Msa1323240:CDS 35.0%
AGAATGATTCATGTACGGTT+GGG - chr8_4:31442297-31442316 Msa1323240:CDS 35.0%
CTAATGAAAGAGCCATACAT+TGG - chr8_4:31442323-31442342 Msa1323240:CDS 35.0%
AAATCTGCGTCGTTGTTTAA+AGG - chr8_4:31442523-31442542 Msa1323240:intron 35.0%
TTGTTTAAAGGTGGAATGTG+AGG - chr8_4:31442535-31442554 Msa1323240:intron 35.0%
CTGGAAAAAGCTCATGATTA+TGG + chr8_4:31442622-31442641 None:intergenic 35.0%
AGGAAATAATGTGCATATGC+TGG + chr8_4:31442641-31442660 None:intergenic 35.0%
CATTATTTCCTTTGCCATAG+AGG - chr8_4:31442650-31442669 Msa1323240:intron 35.0%
!! TATTGATTCCTCTATGGCAA+AGG + chr8_4:31442661-31442680 None:intergenic 35.0%
AAGCAAAATTTATGCCACTC+TGG - chr8_4:31442730-31442749 Msa1323240:CDS 35.0%
GAGAATGATTCATGTACGGT+TGG - chr8_4:31442296-31442315 Msa1323240:CDS 40.0%
ATTACGCTGTCTGTTGGATT+TGG - chr8_4:31442390-31442409 Msa1323240:CDS 40.0%
TTACGCTGTCTGTTGGATTT+GGG - chr8_4:31442391-31442410 Msa1323240:CDS 40.0%
ACTTATGCTCGCACAAGATA+CGG - chr8_4:31442476-31442495 Msa1323240:intron 40.0%
GAGAATGATTCATGTACGGT+TGG - chr8_4:31442296-31442315 Msa1323240:CDS 40.0%
ATTACGCTGTCTGTTGGATT+TGG - chr8_4:31442390-31442409 Msa1323240:CDS 40.0%
TTACGCTGTCTGTTGGATTT+GGG - chr8_4:31442391-31442410 Msa1323240:CDS 40.0%
ACTTATGCTCGCACAAGATA+CGG - chr8_4:31442476-31442495 Msa1323240:intron 40.0%
ATGATTCATGTACGGTTGGG+AGG - chr8_4:31442300-31442319 Msa1323240:CDS 45.0%
ACACTCATTACGCTGTCTGT+TGG - chr8_4:31442384-31442403 Msa1323240:CDS 45.0%
CTTATGCTCGCACAAGATAC+GGG - chr8_4:31442477-31442496 Msa1323240:intron 45.0%
TCTGCGTCGTTGTTTAAAGG+TGG - chr8_4:31442526-31442545 Msa1323240:intron 45.0%
TCCATATTGTTTGGCCAGAG+TGG + chr8_4:31442747-31442766 None:intergenic 45.0%
ACTCTGGCCAAACAATATGG+AGG - chr8_4:31442746-31442765 Msa1323240:CDS 45.0%
ATGATTCATGTACGGTTGGG+AGG - chr8_4:31442300-31442319 Msa1323240:CDS 45.0%
ACACTCATTACGCTGTCTGT+TGG - chr8_4:31442384-31442403 Msa1323240:CDS 45.0%
CTTATGCTCGCACAAGATAC+GGG - chr8_4:31442477-31442496 Msa1323240:intron 45.0%
TCTGCGTCGTTGTTTAAAGG+TGG - chr8_4:31442526-31442545 Msa1323240:intron 45.0%
TCCATATTGTTTGGCCAGAG+TGG + chr8_4:31442747-31442766 None:intergenic 45.0%
ACTCTGGCCAAACAATATGG+AGG - chr8_4:31442746-31442765 Msa1323240:CDS 45.0%
AGCGTGTGTTGAAGGGAGTT+TGG + chr8_4:31441878-31441897 None:intergenic 50.0%
GTGTGTGAGCGTGTGTTGAA+GGG + chr8_4:31441885-31441904 None:intergenic 50.0%
TGTGTGTGAGCGTGTGTTGA+AGG + chr8_4:31441886-31441905 None:intergenic 50.0%
! ACGACGCAGATTTTGTGCAG+TGG + chr8_4:31442516-31442535 None:intergenic 50.0%
GCCACTCTGGCCAAACAATA+TGG - chr8_4:31442743-31442762 Msa1323240:CDS 50.0%
AGCGTGTGTTGAAGGGAGTT+TGG + chr8_4:31441878-31441897 None:intergenic 50.0%
GTGTGTGAGCGTGTGTTGAA+GGG + chr8_4:31441885-31441904 None:intergenic 50.0%
TGTGTGTGAGCGTGTGTTGA+AGG + chr8_4:31441886-31441905 None:intergenic 50.0%
! ACGACGCAGATTTTGTGCAG+TGG + chr8_4:31442516-31442535 None:intergenic 50.0%
GCCACTCTGGCCAAACAATA+TGG - chr8_4:31442743-31442762 Msa1323240:CDS 50.0%
GCACAAGATACGGGAAGACG+CGG - chr8_4:31442486-31442505 Msa1323240:intron 55.0%
CACAAGATACGGGAAGACGC+GGG - chr8_4:31442487-31442506 Msa1323240:intron 55.0%
GCACAAGATACGGGAAGACG+CGG - chr8_4:31442486-31442505 Msa1323240:intron 55.0%
CACAAGATACGGGAAGACGC+GGG - chr8_4:31442487-31442506 Msa1323240:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 31441847 31442832 31441847 ID=Msa1323240;Name=Msa1323240
chr8_4 mRNA 31441847 31442832 31441847 ID=Msa1323240-mRNA-1;Parent=Msa1323240;Name=Msa1323240-mRNA-1;_AED=0.31;_eAED=0.32;_QI=0|0|0|0.66|0|0.33|3|0|219
chr8_4 exon 31442716 31442832 31442716 ID=Msa1323240-mRNA-1:exon:10240;Parent=Msa1323240-mRNA-1
chr8_4 exon 31442282 31442461 31442282 ID=Msa1323240-mRNA-1:exon:10239;Parent=Msa1323240-mRNA-1
chr8_4 exon 31441847 31442209 31441847 ID=Msa1323240-mRNA-1:exon:10238;Parent=Msa1323240-mRNA-1
chr8_4 CDS 31442716 31442832 31442716 ID=Msa1323240-mRNA-1:cds;Parent=Msa1323240-mRNA-1
chr8_4 CDS 31442282 31442461 31442282 ID=Msa1323240-mRNA-1:cds;Parent=Msa1323240-mRNA-1
chr8_4 CDS 31441847 31442209 31441847 ID=Msa1323240-mRNA-1:cds;Parent=Msa1323240-mRNA-1
Gene Sequence

>Msa1323240

ATGAAAATTGGTATTGTCAAATTCTGTGCCAAACTCCCTTCAACACACGCTCACACACAAAAGCCACCGAAGATTTATGTTACAGTAGTGCACTCTGCTATCATTCTTAAAGGTATGGAATTAATGGAAGTTGTTGATGGGAATGATGACAATGTTATTGATGGGAATGAAGAGCAATCTTTCACTGAGATTGTTGAGAATGATTCATGTACGGTTGGGAGGCTAATGAAAGAGCCATACATTGGAATGAAATTTGATTCACAAGAAAATGCTTATTCATTCTACACTCATTACGCTTTATGCACTTATGCTCGCACAAGATACGGGAAGACGCGGGAATCCACTGCACAAAATCTGCGTCGTTGTTTAAAGGTGGAATGTGAGGCTAGCTTGCGTATTAAAAAAAATTGTGATGGAAAATGGATTGTTTACAACTTCATTAAAGACCATAATCATGAGCTTTTTCCAGCATATGCACATTATTTCCTTTGCCATAGAGGAATCAATAAAACTCAAAAGCATTGTATTGAAACTATGCAAGATGTTGGAGTGAGAACAAGCAAAATTTATGCCACTCTGGCCAAACAATATGGAGGATATGAAAACATTGGTTGTTTGCAGAAAGATATCAGAAATCATTTAGATATAGGTCGGTGTTGA

Protein sequence

>Msa1323240

MKIGIVKFCAKLPSTHAHTQKPPKIYVTVVHSAIILKGMELMEVVDGNDDNVIDGNEEQSFTEIVENDSCTVGRLMKEPYIGMKFDSQENAYSFYTHYALCTYARTRYGKTRESTAQNLRRCLKVECEASLRIKKNCDGKWIVYNFIKDHNHELFPAYAHYFLCHRGINKTQKHCIETMQDVGVRTSKIYATLAKQYGGYENIGCLQKDIRNHLDIGRC*