AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1323430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323430 MtrunA17_Chr8g0356071 92.169 166 10 1 1 166 1 163 8.27e-109 307
Msa1323430 MtrunA17_Chr8g0357771 62.712 177 63 1 1 174 1 177 1.54e-75 224
Msa1323430 MtrunA17_Chr1g0169761 57.714 175 71 1 1 172 1 175 2.71e-68 205
Msa1323430 MtrunA17_Chr4g0074101 53.646 192 69 1 1 172 1 192 8.10e-68 205
Msa1323430 MtrunA17_Chr8g0376821 58.683 167 66 1 1 164 1 167 2.83e-65 197
Msa1323430 MtrunA17_Chr1g0180591 54.286 175 77 1 1 172 1 175 1.30e-62 191
Msa1323430 MtrunA17_Chr1g0175601 53.977 176 77 2 1 172 1 176 1.88e-59 183
Msa1323430 MtrunA17_Chr1g0175611 52.976 168 76 1 1 165 1 168 1.38e-58 180
Msa1323430 MtrunA17_Chr4g0052061 38.554 166 83 5 1 161 1 152 3.80e-31 110
Msa1323430 MtrunA17_Chr1g0206991 44.737 114 56 2 2 108 9 122 1.21e-26 99.8
Msa1323430 MtrunA17_Chr3g0099411 39.456 147 68 3 2 141 9 141 1.35e-26 100
Msa1323430 MtrunA17_Chr3g0099421 51.546 97 42 2 2 93 34 130 2.98e-26 100
Msa1323430 MtrunA17_Chr1g0206721 45.614 114 55 2 2 108 9 122 3.10e-26 100
Msa1323430 MtrunA17_Chr3g0119901 45.378 119 57 3 1 114 8 123 4.52e-26 99.0
Msa1323430 MtrunA17_Chr5g0419601 53.846 78 36 0 1 78 8 85 4.59e-25 97.1
Msa1323430 MtrunA17_Chr5g0422941 36.196 163 93 4 2 159 9 165 5.21e-25 96.7
Msa1323430 MtrunA17_Chr3g0085241 43.802 121 63 2 1 116 40 160 9.60e-25 96.3
Msa1323430 MtrunA17_Chr1g0206701 43.590 117 59 2 2 111 9 125 1.04e-24 95.9
Msa1323430 MtrunA17_Chr2g0292791 43.590 117 59 2 2 111 9 125 1.62e-24 95.5
Msa1323430 MtrunA17_Chr1g0188671 57.143 77 33 0 2 78 47 123 3.83e-24 95.5
Msa1323430 MtrunA17_Chr1g0208581 38.095 147 65 2 2 122 9 155 4.27e-24 94.4
Msa1323430 MtrunA17_Chr8g0355091 45.545 101 50 1 2 97 9 109 6.54e-24 94.0
Msa1323430 MtrunA17_Chr3g0089141 42.149 121 65 2 2 117 9 129 7.18e-24 93.6
Msa1323430 MtrunA17_Chr1g0149391 37.423 163 87 3 2 149 9 171 7.67e-24 93.6
Msa1323430 MtrunA17_Chr1g0187361 55.844 77 34 0 2 78 9 85 1.33e-23 93.2
Msa1323430 MtrunA17_Chr2g0333981 35.762 151 88 4 1 150 11 153 1.36e-23 91.7
Msa1323430 MtrunA17_Chr1g0208561 45.045 111 55 3 2 107 9 118 1.38e-23 92.8
Msa1323430 MtrunA17_Chr4g0052081 51.316 76 37 0 1 76 1 76 1.45e-23 91.7
Msa1323430 MtrunA17_Chr2g0325971 37.415 147 66 1 2 122 9 155 1.82e-23 92.4
Msa1323430 MtrunA17_Chr2g0300441 35.220 159 88 2 2 145 9 167 2.03e-23 92.4
Msa1323430 MtrunA17_Chr5g0422421 53.247 77 36 0 2 78 9 85 9.07e-23 90.9
Msa1323430 MtrunA17_Chr4g0018951 37.879 132 66 3 17 135 27 155 1.09e-22 89.0
Msa1323430 MtrunA17_Chr3g0089151 51.948 77 37 0 2 78 9 85 2.17e-22 87.0
Msa1323430 MtrunA17_Chr8g0379021 37.226 137 78 4 2 136 12 142 2.62e-22 88.2
Msa1323430 MtrunA17_Chr2g0317451 53.247 77 36 0 2 78 9 85 2.69e-22 89.7
Msa1323430 MtrunA17_Chr2g0317481 53.247 77 36 0 2 78 9 85 2.83e-22 89.7
Msa1323430 MtrunA17_Chr2g0300421 41.441 111 59 2 2 107 9 118 2.79e-21 85.5
Msa1323430 MtrunA17_Chr1g0206691 41.593 113 57 2 18 125 8 116 9.17e-21 85.1
Msa1323430 MtrunA17_Chr5g0404951 53.125 64 30 0 1 64 1 64 1.50e-19 82.4
Msa1323430 MtrunA17_Chr4g0020221 52.174 69 33 0 1 69 1 69 3.38e-19 82.0
Msa1323430 MtrunA17_Chr1g0187351 60.000 55 22 0 18 72 8 62 1.03e-18 75.9
Msa1323430 MtrunA17_Chr5g0427401 48.611 72 37 0 1 72 1 72 3.06e-18 78.6
Msa1323430 MtrunA17_Chr7g0238991 55.556 63 28 0 16 78 35 97 5.51e-18 78.6
Msa1323430 MtrunA17_Chr3g0097101 31.875 160 105 2 1 159 16 172 1.60e-17 77.0
Msa1323430 MtrunA17_Chr4g0071601 47.436 78 41 0 1 78 11 88 1.61e-17 75.9
Msa1323430 MtrunA17_Chr8g0380021 34.862 109 71 0 1 109 17 125 2.61e-17 76.6
Msa1323430 MtrunA17_Chr2g0279821 40.230 87 51 1 1 87 1 86 5.15e-17 75.9
Msa1323430 MtrunA17_Chr5g0427921 55.000 60 27 0 1 60 1 60 5.42e-17 71.2
Msa1323430 MtrunA17_Chr5g0411691 47.826 69 36 0 1 69 1 69 7.10e-17 75.5
Msa1323430 MtrunA17_Chr4g0049901 38.462 117 63 2 1 108 27 143 8.21e-17 75.1
Msa1323430 MtrunA17_Chr8g0351041 36.893 103 62 2 1 101 1 102 9.07e-17 73.6
Msa1323430 MtrunA17_Chr1g0173161 46.250 80 40 1 1 80 1 77 1.13e-16 71.2
Msa1323430 MtrunA17_Chr4g0049541 44.928 69 38 0 1 69 1 69 1.44e-16 74.3
Msa1323430 MtrunA17_Chr8g0351051 46.377 69 37 0 1 69 1 69 1.63e-16 74.7
Msa1323430 MtrunA17_Chr3g0077501 44.928 69 38 0 1 69 1 69 2.22e-16 73.6
Msa1323430 MtrunA17_Chr2g0284911 33.028 109 73 0 1 109 16 124 2.97e-16 73.9
Msa1323430 MtrunA17_Chr3g0111931 50.820 61 30 0 18 78 6 66 3.87e-16 70.5
Msa1323430 MtrunA17_Chr3g0111841 58.333 48 20 0 18 65 12 59 4.78e-16 68.9
Msa1323430 MtrunA17_Chr8g0351031 36.893 103 62 2 1 101 1 102 4.86e-16 72.8
Msa1323430 MtrunA17_Chr3g0142191 48.611 72 35 1 1 72 1 70 7.38e-16 72.4
Msa1323430 MtrunA17_Chr7g0246431 33.663 101 67 0 1 101 1 101 1.26e-15 71.6
Msa1323430 MtrunA17_Chr5g0412271 42.857 84 47 1 1 84 1 83 1.32e-15 71.6
Msa1323430 MtrunA17_Chr5g0412461 42.857 84 47 1 1 84 1 83 1.72e-15 71.2
Msa1323430 MtrunA17_Chr1g0164791 46.479 71 36 1 1 69 1 71 3.66e-15 70.5
Msa1323430 MtrunA17_Chr4g0044071 55.172 58 26 0 1 58 1 58 6.01e-15 67.4
Msa1323430 MtrunA17_Chr4g0057151 43.478 69 39 0 1 69 1 69 6.07e-15 69.7
Msa1323430 MtrunA17_Chr2g0308251 40.698 86 46 2 1 85 1 82 1.03e-14 65.9
Msa1323430 MtrunA17_Chr4g0018421 44.444 72 39 1 1 71 1 72 1.26e-14 68.6
Msa1323430 MtrunA17_Chr5g0420091 50.877 57 28 0 1 57 1 57 1.38e-14 68.9
Msa1323430 MtrunA17_Chr7g0220111 50.877 57 28 0 1 57 1 57 1.69e-14 68.9
Msa1323430 MtrunA17_Chr7g0246381 47.059 68 31 1 1 63 1 68 2.12e-14 64.7
Msa1323430 MtrunA17_Chr4g0061671 48.276 58 30 0 1 58 1 58 2.41e-14 67.4
Msa1323430 MtrunA17_Chr1g0159661 35.345 116 58 3 1 114 1 101 2.70e-14 67.4
Msa1323430 MtrunA17_Chr1g0204111 43.662 71 40 0 1 71 17 87 2.72e-14 68.6
Msa1323430 MtrunA17_Chr4g0061691 36.170 94 47 2 1 81 1 94 3.46e-14 68.2
Msa1323430 MtrunA17_Chr5g0425191 58.491 53 22 0 1 53 1 53 4.29e-14 65.1
Msa1323430 MtrunA17_Chr8g0377561 50.877 57 28 0 1 57 1 57 4.49e-14 64.7
Msa1323430 MtrunA17_Chr3g0122251 52.632 57 27 0 1 57 1 57 5.31e-14 67.8
Msa1323430 MtrunA17_Chr8g0366231 41.667 72 39 1 1 72 1 69 5.36e-14 67.4
Msa1323430 MtrunA17_Chr6g0455511 50.877 57 28 0 1 57 1 57 8.18e-14 67.4
Msa1323430 MtrunA17_Chr7g0220121 38.356 73 44 1 1 72 1 73 1.10e-13 67.0
Msa1323430 MtrunA17_Chr3g0125241 41.538 65 38 0 1 65 26 90 1.36e-13 65.9
Msa1323430 MtrunA17_Chr3g0134451 42.254 71 40 1 1 70 1 71 1.52e-13 67.0
Msa1323430 MtrunA17_Chr5g0420081 45.614 57 31 0 1 57 1 57 1.68e-13 66.2
Msa1323430 MtrunA17_Chr8g0345001 36.893 103 54 2 20 122 36 127 2.04e-13 65.1
Msa1323430 MtrunA17_Chr6g0474371 40.278 72 42 1 1 71 1 72 6.14e-13 63.9
Msa1323430 MtrunA17_Chr3g0093131 42.466 73 33 2 19 82 36 108 9.52e-13 63.5
Msa1323430 MtrunA17_Chr3g0111831 52.083 48 23 0 18 65 20 67 1.40e-12 60.5
Msa1323430 MtrunA17_Chr1g0166611 60.870 46 18 0 13 58 17 62 1.63e-12 61.6
Msa1323430 MtrunA17_Chr5g0417661 50.000 54 27 0 1 54 56 109 1.92e-12 61.2
Msa1323430 MtrunA17_Chr4g0025531 31.250 96 54 2 19 108 26 115 1.08e-11 61.2
Msa1323430 MtrunA17_Chr4g0060841 30.075 133 66 2 1 106 1 133 1.48e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323430 AT2G34440.1 38.596 171 92 2 1 158 1 171 1.35e-35 122
Msa1323430 AT3G66656.1 48.819 127 59 2 1 121 1 127 1.19e-31 112
Msa1323430 AT2G24840.1 43.548 124 59 3 1 117 62 181 3.75e-28 105
Msa1323430 AT5G60440.1 43.662 142 69 4 2 136 7 144 2.41e-27 104
Msa1323430 AT4G36590.1 38.554 166 77 5 2 157 7 157 9.56e-25 96.7
Msa1323430 AT1G01530.1 41.007 139 75 2 1 136 6 140 3.90e-24 95.1
Msa1323430 AT1G65360.1 38.621 145 70 4 1 136 6 140 2.67e-22 89.7
Msa1323430 AT1G72350.1 40.496 121 65 3 2 122 43 156 2.36e-20 84.7
Msa1323430 AT1G47760.1 31.056 161 101 3 1 154 1 158 8.40e-20 82.4
Msa1323430 AT3G61120.1 44.578 83 44 1 1 81 1 83 2.27e-19 82.4
Msa1323430 AT5G65070.4 39.496 119 68 4 1 116 1 118 2.73e-19 80.1
Msa1323430 AT4G24540.1 40.230 87 52 0 1 87 1 87 5.99e-19 80.9
Msa1323430 AT5G65070.1 37.857 140 80 5 1 134 1 139 7.00e-19 80.1
Msa1323430 AT5G13790.2 37.705 122 54 1 1 122 1 100 1.38e-18 80.9
Msa1323430 AT5G13790.1 40.206 97 58 0 1 97 1 97 2.44e-18 80.1
Msa1323430 AT3G57390.3 50.725 69 34 0 1 69 1 69 5.49e-18 78.6
Msa1323430 AT1G28460.1 46.377 69 37 0 2 70 8 76 6.43e-18 77.4
Msa1323430 AT1G29962.1 45.833 72 39 0 2 73 8 79 6.84e-18 77.4
Msa1323430 AT3G57390.1 50.725 69 34 0 1 69 1 69 9.20e-18 78.6
Msa1323430 AT3G57230.2 46.377 69 37 0 1 69 1 69 1.21e-17 75.5
Msa1323430 AT4G22950.2 47.826 69 36 0 1 69 1 69 1.83e-17 75.9
Msa1323430 AT3G57230.1 46.377 69 37 0 1 69 1 69 2.49e-17 77.0
Msa1323430 AT3G57230.4 46.377 69 37 0 1 69 1 69 2.49e-17 77.0
Msa1323430 AT4G09960.6 30.065 153 93 4 1 147 2 146 2.74e-17 76.6
Msa1323430 AT2G22540.3 39.423 104 61 2 1 103 1 103 2.76e-17 76.6
Msa1323430 AT2G22540.1 39.423 104 61 2 1 103 1 103 2.76e-17 76.6
Msa1323430 AT4G09960.1 30.065 153 93 4 1 147 1 145 2.78e-17 76.6
Msa1323430 AT5G65080.3 35.915 142 85 3 1 136 8 149 3.86e-17 75.5
Msa1323430 AT4G09960.2 38.462 91 51 1 1 91 1 86 4.07e-17 75.9
Msa1323430 AT4G09960.5 30.065 153 93 4 1 147 11 155 4.63e-17 76.3
Msa1323430 AT2G45650.1 46.377 69 37 0 1 69 1 69 4.72e-17 76.3
Msa1323430 AT5G65070.2 40.426 94 54 1 1 94 1 92 5.38e-17 75.9
Msa1323430 AT4G09960.3 30.065 153 93 4 1 147 27 171 6.13e-17 76.3
Msa1323430 AT5G65070.5 40.426 94 54 1 1 94 1 92 6.37e-17 75.1
Msa1323430 AT2G22630.1 49.275 69 35 0 1 69 1 69 6.75e-17 75.5
Msa1323430 AT2G22630.2 49.275 69 35 0 1 69 1 69 6.75e-17 75.5
Msa1323430 AT2G45660.2 34.286 105 69 0 1 105 1 105 9.38e-17 73.6
Msa1323430 AT3G04100.1 43.421 76 40 1 2 74 14 89 1.03e-16 74.7
Msa1323430 AT4G09960.4 38.462 91 51 1 1 91 96 181 1.19e-16 76.3
Msa1323430 AT5G10140.2 54.386 57 26 0 1 57 1 57 1.61e-16 73.2
Msa1323430 AT4G22950.3 47.826 69 36 0 1 69 1 69 1.62e-16 74.3
Msa1323430 AT4G22950.1 47.826 69 36 0 1 69 1 69 1.62e-16 74.3
Msa1323430 AT1G17310.1 58.621 58 22 1 17 74 63 118 1.78e-16 74.3
Msa1323430 AT1G17310.2 58.621 58 22 1 17 74 74 129 2.26e-16 74.3
Msa1323430 AT5G10140.3 53.448 58 27 0 1 58 1 58 2.93e-16 73.2
Msa1323430 AT4G37940.1 46.377 69 37 0 1 69 1 69 3.05e-16 73.9
Msa1323430 AT5G10140.4 54.386 57 26 0 1 57 1 57 3.07e-16 72.8
Msa1323430 AT2G45660.1 34.286 105 69 0 1 105 1 105 3.16e-16 73.6
Msa1323430 AT5G65070.3 45.070 71 37 1 1 69 1 71 3.31e-16 73.9
Msa1323430 AT3G54340.1 30.108 186 101 5 1 164 1 179 3.60e-16 73.6
Msa1323430 AT5G10140.1 54.386 57 26 0 1 57 1 57 3.61e-16 73.2
Msa1323430 AT5G51870.2 46.377 69 37 0 1 69 1 69 4.08e-16 72.4
Msa1323430 AT4G37940.4 46.377 69 37 0 1 69 1 69 4.23e-16 73.2
Msa1323430 AT5G51870.1 46.377 69 37 0 1 69 1 69 4.30e-16 73.2
Msa1323430 AT5G51870.5 46.377 69 37 0 1 69 24 92 6.70e-16 72.4
Msa1323430 AT5G51870.4 46.377 69 37 0 1 69 1 69 7.95e-16 72.4
Msa1323430 AT4G37940.3 43.210 81 42 1 1 77 1 81 8.49e-16 71.6
Msa1323430 AT5G51870.3 46.377 69 37 0 1 69 11 79 1.28e-15 72.0
Msa1323430 AT2G22540.2 56.364 55 24 0 1 55 1 55 1.55e-15 72.0
Msa1323430 AT2G42830.1 38.298 94 53 2 1 94 16 104 1.71e-15 72.0
Msa1323430 AT2G42830.2 38.298 94 53 2 1 94 16 104 1.75e-15 72.0
Msa1323430 AT5G51860.2 46.377 69 37 0 1 69 1 69 1.92e-15 71.2
Msa1323430 AT3G58780.1 38.298 94 53 2 1 94 16 104 2.03e-15 72.0
Msa1323430 AT5G65060.3 56.364 55 24 0 1 55 1 55 2.16e-15 70.9
Msa1323430 AT3G58780.5 38.298 94 53 2 1 94 22 110 2.21e-15 72.0
Msa1323430 AT5G51860.1 46.377 69 37 0 1 69 1 69 2.28e-15 71.2
Msa1323430 AT4G11880.2 53.448 58 27 0 1 58 1 58 2.31e-15 70.5
Msa1323430 AT4G11880.3 53.448 58 27 0 1 58 1 58 2.31e-15 70.5
Msa1323430 AT5G65050.2 54.545 55 25 0 1 55 1 55 2.74e-15 70.1
Msa1323430 AT5G20240.2 49.206 63 32 0 1 63 1 63 2.77e-15 69.7
Msa1323430 AT3G58780.2 40.698 86 38 1 1 73 16 101 3.45e-15 71.2
Msa1323430 AT1G22130.1 49.296 71 35 1 1 70 1 71 3.53e-15 72.4
Msa1323430 AT5G65060.1 56.364 55 24 0 1 55 1 55 3.62e-15 70.5
Msa1323430 AT3G58780.4 38.298 94 53 2 1 94 16 104 3.93e-15 71.6
Msa1323430 AT5G65060.2 56.364 55 24 0 1 55 1 55 3.94e-15 70.1
Msa1323430 AT5G23260.2 59.649 57 23 0 1 57 1 57 5.29e-15 70.9
Msa1323430 AT5G23260.1 59.649 57 23 0 1 57 17 73 5.44e-15 70.9
Msa1323430 AT2G14210.1 53.448 58 27 0 1 58 1 58 5.46e-15 70.5
Msa1323430 AT5G23260.4 59.649 57 23 0 1 57 17 73 5.75e-15 70.9
Msa1323430 AT1G71692.1 50.000 62 31 0 1 62 1 62 5.98e-15 70.1
Msa1323430 AT5G20240.1 49.206 63 32 0 1 63 1 63 6.19e-15 70.1
Msa1323430 AT5G23260.3 59.649 57 23 0 1 57 1 57 6.20e-15 70.5
Msa1323430 AT5G62165.17 40.000 75 45 0 1 75 1 75 6.60e-15 68.9
Msa1323430 AT5G62165.12 40.000 75 45 0 1 75 1 75 6.60e-15 68.9
Msa1323430 AT5G62165.11 40.000 75 45 0 1 75 1 75 6.60e-15 68.9
Msa1323430 AT4G11880.4 53.448 58 27 0 1 58 1 58 6.63e-15 70.1
Msa1323430 AT4G11880.1 53.448 58 27 0 1 58 1 58 6.63e-15 70.1
Msa1323430 AT2G14210.2 53.448 58 27 0 1 58 1 58 7.23e-15 70.1
Msa1323430 AT5G62165.14 40.000 75 45 0 1 75 1 75 9.12e-15 67.0
Msa1323430 AT4G18960.1 31.538 130 84 2 2 131 18 142 9.57e-15 70.1
Msa1323430 AT5G62165.6 50.877 57 28 0 1 57 1 57 9.84e-15 68.2
Msa1323430 AT5G62165.9 50.877 57 28 0 1 57 1 57 9.84e-15 68.2
Msa1323430 AT5G62165.7 50.877 57 28 0 1 57 1 57 9.84e-15 68.2
Msa1323430 AT5G62165.8 50.877 57 28 0 1 57 1 57 9.84e-15 68.2
Msa1323430 AT5G62165.13 50.877 57 28 0 1 57 1 57 9.84e-15 68.2
Msa1323430 AT1G77980.1 47.887 71 36 1 1 70 1 71 1.16e-14 70.9
Msa1323430 AT2G03710.3 54.386 57 26 0 1 57 1 57 1.21e-14 68.9
Msa1323430 AT5G65050.3 54.545 55 25 0 1 55 1 55 1.33e-14 68.9
Msa1323430 AT5G65050.5 54.545 55 25 0 1 55 1 55 1.41e-14 68.6
Msa1323430 AT5G65050.1 54.545 55 25 0 1 55 1 55 1.45e-14 68.6
Msa1323430 AT5G62165.10 40.000 75 45 0 1 75 1 75 1.60e-14 68.6
Msa1323430 AT5G62165.4 40.000 75 45 0 1 75 1 75 1.60e-14 68.6
Msa1323430 AT5G62165.3 42.029 69 40 0 1 69 1 69 1.73e-14 68.9
Msa1323430 AT5G62165.1 42.029 69 40 0 1 69 1 69 1.73e-14 68.9
Msa1323430 AT5G62165.2 42.029 69 40 0 1 69 1 69 1.73e-14 68.9
Msa1323430 AT5G65080.2 50.909 55 27 0 1 55 8 62 2.53e-14 68.2
Msa1323430 AT3G58780.3 50.847 59 29 0 1 59 16 74 3.02e-14 68.9
Msa1323430 AT3G30260.1 49.123 57 29 0 1 57 1 57 3.07e-14 68.9
Msa1323430 AT1G69120.2 43.056 72 38 1 1 72 1 69 3.99e-14 68.2
Msa1323430 AT2G03710.2 54.386 57 26 0 1 57 1 57 4.16e-14 68.6
Msa1323430 AT2G03710.1 54.386 57 26 0 1 57 1 57 4.43e-14 68.6
Msa1323430 AT1G46408.1 48.148 81 35 4 1 74 1 81 7.14e-14 67.8
Msa1323430 AT5G15800.1 50.877 57 28 0 1 57 1 57 7.68e-14 67.8
Msa1323430 AT4G37435.1 30.058 173 93 6 1 147 1 171 8.06e-14 66.6
Msa1323430 AT1G69120.1 43.056 72 38 1 1 72 1 69 8.29e-14 67.8
Msa1323430 AT3G02310.2 50.877 57 28 0 1 57 1 57 8.54e-14 66.6
Msa1323430 AT1G28450.1 40.278 72 43 0 2 73 8 79 9.17e-14 66.2
Msa1323430 AT5G15800.2 50.877 57 28 0 1 57 1 57 1.21e-13 67.4
Msa1323430 AT5G60910.1 40.278 72 40 1 1 72 1 69 1.33e-13 67.0
Msa1323430 AT1G24260.1 50.877 57 28 0 1 57 1 57 1.50e-13 67.0
Msa1323430 AT1G24260.3 50.877 57 28 0 1 57 1 57 1.50e-13 66.6
Msa1323430 AT1G24260.2 50.877 57 28 0 1 57 1 57 1.56e-13 67.0
Msa1323430 AT3G02310.1 50.877 57 28 0 1 57 1 57 1.64e-13 66.6
Msa1323430 AT1G26310.1 46.552 58 31 0 1 58 1 58 1.94e-13 66.6
Msa1323430 AT1G18750.4 40.789 76 43 1 1 74 1 76 1.04e-12 65.5
Msa1323430 AT1G18750.3 40.789 76 43 1 1 74 1 76 1.05e-12 65.5
Msa1323430 AT1G18750.1 40.789 76 43 1 1 74 1 76 1.20e-12 65.5
Msa1323430 AT5G49490.1 44.444 72 36 1 3 70 46 117 1.77e-12 64.3
Msa1323430 AT1G69540.1 40.000 75 44 1 1 74 1 75 1.89e-12 64.7
Msa1323430 AT5G38620.1 43.056 72 37 1 3 70 6 77 2.06e-12 64.3
Msa1323430 AT1G69540.2 40.000 75 44 1 1 74 1 75 2.36e-12 64.3
Msa1323430 AT5G49420.1 42.857 77 39 2 3 74 8 84 2.77e-12 63.9
Msa1323430 AT5G04640.1 40.260 77 41 2 3 74 5 81 3.09e-12 63.9
Msa1323430 AT1G77950.3 38.028 71 43 1 1 70 1 71 8.10e-12 62.0
Msa1323430 AT1G77950.2 38.028 71 43 1 1 70 1 71 8.10e-12 62.0
Msa1323430 AT1G77950.1 38.028 71 43 1 1 70 1 71 8.10e-12 62.0
Msa1323430 AT1G77950.4 38.028 71 43 1 1 70 1 71 8.10e-12 62.0
Msa1323430 AT2G26320.1 44.828 58 32 0 1 58 17 74 2.03e-11 58.5
Msa1323430 AT1G77080.11 66.667 39 13 0 1 39 1 39 2.64e-11 59.7
Msa1323430 AT1G77080.5 66.667 39 13 0 1 39 1 39 3.03e-11 59.3
Msa1323430 AT2G03060.3 50.943 53 26 0 1 53 1 53 3.14e-11 61.2
Msa1323430 AT2G03060.7 50.943 53 26 0 1 53 1 53 3.14e-11 61.2
Msa1323430 AT2G03060.1 50.943 53 26 0 1 53 1 53 3.14e-11 61.2
Msa1323430 AT2G03060.2 50.943 53 26 0 1 53 1 53 3.38e-11 61.2
Msa1323430 AT1G77080.12 66.667 39 13 0 1 39 1 39 4.89e-11 58.2
Msa1323430 AT1G77080.4 66.667 39 13 0 1 39 1 39 6.51e-11 58.9
Msa1323430 AT1G77080.2 66.667 39 13 0 1 39 1 39 9.74e-11 58.5

Find 47 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATACAACAATAGCAATTT+TGG 0.202437 8_4:+31762770 None:intergenic
GGGAATAAACCTTACTCTTT+TGG 0.260036 8_4:-31762741 Msa1323430:CDS
CCATCACCTTCTCATGTTGT+TGG 0.285658 8_4:+31762619 None:intergenic
TGTTAGAGGAAGGGGTTTCT+AGG 0.312060 8_4:+31762655 None:intergenic
AATGTGACTTGTCTCGCCTC+TGG 0.338519 8_4:+31762852 None:intergenic
ATTAATGGAGCTCAAGGATA+TGG 0.358058 8_4:-31762496 Msa1323430:CDS
GGAATGGAAAGATTCATTAA+TGG 0.365090 8_4:-31762511 Msa1323430:CDS
ATTCCTTAGGCTGGCTGAGT+AGG 0.404026 8_4:+31762529 None:intergenic
ACATTCTCAAAGCGCCGAAT+GGG 0.416565 8_4:-31762834 Msa1323430:CDS
TTCTAGGACATCGTTTAGAT+CGG 0.426926 8_4:+31762671 None:intergenic
GTTCAGATCATCTATGTTAG+AGG 0.427003 8_4:+31762641 None:intergenic
GCTATTGTTGTATTCTCTCC+CGG 0.429351 8_4:-31762762 Msa1323430:CDS
ATCATCTATGTTAGAGGAAG+GGG 0.438239 8_4:+31762647 None:intergenic
TGATATAGCTGAGGCTGAAA+AGG 0.444985 8_4:-31762595 Msa1323430:CDS
AATCTTTCCATTCCTTAGGC+TGG 0.454254 8_4:+31762520 None:intergenic
AAAAGAGTAAGGTTTATTCC+CGG 0.457419 8_4:+31762743 None:intergenic
TCTAGGACATCGTTTAGATC+GGG 0.468128 8_4:+31762672 None:intergenic
ATGTGACTTGTCTCGCCTCT+GGG 0.475748 8_4:+31762853 None:intergenic
TTCCTTAGGCTGGCTGAGTA+GGG 0.493210 8_4:+31762530 None:intergenic
CACATTCTCAAAGCGCCGAA+TGG 0.503482 8_4:-31762835 Msa1323430:CDS
CCAGGGTGGCCAAAAGAGTA+AGG 0.504633 8_4:+31762732 None:intergenic
ACAATTAACTTATTATGGGT+CGG 0.510146 8_4:-31762905 None:intergenic
TATGCTAAAAGAATACAAAT+CGG 0.512468 8_4:-31762553 Msa1323430:CDS
ACGAGCTTGCCATCTTGTGT+GGG 0.513990 8_4:-31762794 Msa1323430:CDS
AATGAATCTTTCCATTCCTT+AGG 0.516915 8_4:+31762516 None:intergenic
AACGAGCTTGCCATCTTGTG+TGG 0.526962 8_4:-31762795 Msa1323430:CDS
AGATTCATTAATGGAGCTCA+AGG 0.527990 8_4:-31762502 Msa1323430:CDS
CTATTGTTGTATTCTCTCCC+GGG 0.529578 8_4:-31762761 Msa1323430:CDS
TGAGGCTGAAAAGGAAGATA+AGG 0.530489 8_4:-31762586 Msa1323430:CDS
TTCTATGATGCTCCTTGCAA+AGG 0.533227 8_4:-31762439 Msa1323430:CDS
CAACCACGGGCTCCTTTGCA+AGG 0.540458 8_4:+31762427 None:intergenic
AACCAAGCCACAGAAAAGAA+AGG 0.541403 8_4:-31762396 Msa1323430:CDS
GACGCAATAGCATCAACACC+AGG 0.542737 8_4:+31762714 None:intergenic
AAAGAGTAAGGTTTATTCCC+GGG 0.547640 8_4:+31762744 None:intergenic
AGATCATCTATGTTAGAGGA+AGG 0.556275 8_4:+31762645 None:intergenic
GATCATCTATGTTAGAGGAA+GGG 0.557711 8_4:+31762646 None:intergenic
CTTGCAAAGGAGCCCGTGGT+TGG 0.559070 8_4:-31762426 Msa1323430:CDS
TACTCAGCCAGCCTAAGGAA+TGG 0.565385 8_4:-31762527 Msa1323430:CDS
TCTGAACCAACAACATGAGA+AGG 0.616384 8_4:-31762625 Msa1323430:CDS
AAAGGAAGATAAGGCCAATG+AGG 0.641735 8_4:-31762577 Msa1323430:CDS
GCGCAAAATTGAGATTGCGA+TGG 0.644560 8_4:-31762883 Msa1323430:CDS
CATTCTCAAAGCGCCGAATG+GGG 0.649352 8_4:-31762833 Msa1323430:CDS
GCTCCTTGCAAAGGAGCCCG+TGG 0.674787 8_4:-31762430 Msa1323430:CDS
CCAACAACATGAGAAGGTGA+TGG 0.695579 8_4:-31762619 Msa1323430:CDS
GGTGATGGCTGATATAGCTG+AGG 0.696275 8_4:-31762604 Msa1323430:CDS
ACGCAATAGCATCAACACCA+GGG 0.722765 8_4:+31762715 None:intergenic
CAATAGCATCAACACCAGGG+TGG 0.755219 8_4:+31762718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGCTAAAAGAATACAAAT+CGG - chr8_4:31762724-31762743 Msa1323430:CDS 20.0%
!! GAATACAACAATAGCAATTT+TGG + chr8_4:31762510-31762529 None:intergenic 25.0%
!! ATATTTTCCTTTCTTTTCTG+TGG + chr8_4:31762891-31762910 None:intergenic 25.0%
!!! CTTTTTTGAATAACCCCATT+CGG + chr8_4:31762460-31762479 None:intergenic 30.0%
AAAAGAGTAAGGTTTATTCC+CGG + chr8_4:31762537-31762556 None:intergenic 30.0%
! TTCTTTTAGCATATCCTCAT+TGG + chr8_4:31762717-31762736 None:intergenic 30.0%
AATGAATCTTTCCATTCCTT+AGG + chr8_4:31762764-31762783 None:intergenic 30.0%
GGAATGGAAAGATTCATTAA+TGG - chr8_4:31762766-31762785 Msa1323430:CDS 30.0%
!! TTGGTTTTTAATTCCAACCA+CGG + chr8_4:31762867-31762886 None:intergenic 30.0%
AAAGAGTAAGGTTTATTCCC+GGG + chr8_4:31762536-31762555 None:intergenic 35.0%
! GGGAATAAACCTTACTCTTT+TGG - chr8_4:31762536-31762555 Msa1323430:CDS 35.0%
!! TTCTAGGACATCGTTTAGAT+CGG + chr8_4:31762609-31762628 None:intergenic 35.0%
ATCATCTATGTTAGAGGAAG+GGG + chr8_4:31762633-31762652 None:intergenic 35.0%
GATCATCTATGTTAGAGGAA+GGG + chr8_4:31762634-31762653 None:intergenic 35.0%
AGATCATCTATGTTAGAGGA+AGG + chr8_4:31762635-31762654 None:intergenic 35.0%
GTTCAGATCATCTATGTTAG+AGG + chr8_4:31762639-31762658 None:intergenic 35.0%
AGATTCATTAATGGAGCTCA+AGG - chr8_4:31762775-31762794 Msa1323430:CDS 35.0%
ATTAATGGAGCTCAAGGATA+TGG - chr8_4:31762781-31762800 Msa1323430:CDS 35.0%
!! TGGTTTTTAATTCCAACCAC+GGG + chr8_4:31762866-31762885 None:intergenic 35.0%
GCTATTGTTGTATTCTCTCC+CGG - chr8_4:31762515-31762534 Msa1323430:CDS 40.0%
CTATTGTTGTATTCTCTCCC+GGG - chr8_4:31762516-31762535 Msa1323430:CDS 40.0%
! TCTAGGACATCGTTTAGATC+GGG + chr8_4:31762608-31762627 None:intergenic 40.0%
TCTGAACCAACAACATGAGA+AGG - chr8_4:31762652-31762671 Msa1323430:CDS 40.0%
TGATATAGCTGAGGCTGAAA+AGG - chr8_4:31762682-31762701 Msa1323430:CDS 40.0%
TGAGGCTGAAAAGGAAGATA+AGG - chr8_4:31762691-31762710 Msa1323430:CDS 40.0%
AAAGGAAGATAAGGCCAATG+AGG - chr8_4:31762700-31762719 Msa1323430:CDS 40.0%
AATCTTTCCATTCCTTAGGC+TGG + chr8_4:31762760-31762779 None:intergenic 40.0%
TTCTATGATGCTCCTTGCAA+AGG - chr8_4:31762838-31762857 Msa1323430:CDS 40.0%
AACCAAGCCACAGAAAAGAA+AGG - chr8_4:31762881-31762900 Msa1323430:CDS 40.0%
! TTCCTTTCTTTTCTGTGGCT+TGG + chr8_4:31762886-31762905 None:intergenic 40.0%
GCGCAAAATTGAGATTGCGA+TGG - chr8_4:31762394-31762413 Msa1323430:CDS 45.0%
!! ACATTCTCAAAGCGCCGAAT+GGG - chr8_4:31762443-31762462 Msa1323430:CDS 45.0%
!! AATTTTGGCCCCACACAAGA+TGG + chr8_4:31762495-31762514 None:intergenic 45.0%
ACGCAATAGCATCAACACCA+GGG + chr8_4:31762565-31762584 None:intergenic 45.0%
TGTTAGAGGAAGGGGTTTCT+AGG + chr8_4:31762625-31762644 None:intergenic 45.0%
CCATCACCTTCTCATGTTGT+TGG + chr8_4:31762661-31762680 None:intergenic 45.0%
CCAACAACATGAGAAGGTGA+TGG - chr8_4:31762658-31762677 Msa1323430:CDS 45.0%
TGCGATGGTGAAAAACCCAG+AGG - chr8_4:31762409-31762428 Msa1323430:CDS 50.0%
! ATGTGACTTGTCTCGCCTCT+GGG + chr8_4:31762427-31762446 None:intergenic 50.0%
! AATGTGACTTGTCTCGCCTC+TGG + chr8_4:31762428-31762447 None:intergenic 50.0%
!! CACATTCTCAAAGCGCCGAA+TGG - chr8_4:31762442-31762461 Msa1323430:CDS 50.0%
!! CATTCTCAAAGCGCCGAATG+GGG - chr8_4:31762444-31762463 Msa1323430:CDS 50.0%
AACGAGCTTGCCATCTTGTG+TGG - chr8_4:31762482-31762501 Msa1323430:CDS 50.0%
ACGAGCTTGCCATCTTGTGT+GGG - chr8_4:31762483-31762502 Msa1323430:CDS 50.0%
CAATAGCATCAACACCAGGG+TGG + chr8_4:31762562-31762581 None:intergenic 50.0%
GACGCAATAGCATCAACACC+AGG + chr8_4:31762566-31762585 None:intergenic 50.0%
! GGTGATGGCTGATATAGCTG+AGG - chr8_4:31762673-31762692 Msa1323430:CDS 50.0%
! TTCCTTAGGCTGGCTGAGTA+GGG + chr8_4:31762750-31762769 None:intergenic 50.0%
! ATTCCTTAGGCTGGCTGAGT+AGG + chr8_4:31762751-31762770 None:intergenic 50.0%
TACTCAGCCAGCCTAAGGAA+TGG - chr8_4:31762750-31762769 Msa1323430:CDS 50.0%
CGAGCTTGCCATCTTGTGTG+GGG - chr8_4:31762484-31762503 Msa1323430:CDS 55.0%
CCAGGGTGGCCAAAAGAGTA+AGG + chr8_4:31762548-31762567 None:intergenic 55.0%
! CCTTACTCTTTTGGCCACCC+TGG - chr8_4:31762545-31762564 Msa1323430:CDS 55.0%
CAACCACGGGCTCCTTTGCA+AGG + chr8_4:31762853-31762872 None:intergenic 60.0%
CTTGCAAAGGAGCCCGTGGT+TGG - chr8_4:31762851-31762870 Msa1323430:CDS 60.0%
GGCCCTACTCAGCCAGCCTA+AGG - chr8_4:31762745-31762764 Msa1323430:CDS 65.0%
GCTCCTTGCAAAGGAGCCCG+TGG - chr8_4:31762847-31762866 Msa1323430:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 31762386 31762913 31762386 ID=Msa1323430;Name=Msa1323430
chr8_4 mRNA 31762386 31762913 31762386 ID=Msa1323430-mRNA-1;Parent=Msa1323430;Name=Msa1323430-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|175
chr8_4 exon 31762386 31762913 31762386 ID=Msa1323430-mRNA-1:exon:10336;Parent=Msa1323430-mRNA-1
chr8_4 CDS 31762386 31762913 31762386 ID=Msa1323430-mRNA-1:cds;Parent=Msa1323430-mRNA-1
Gene Sequence

>Msa1323430

ATGGGTCGGCGCAAAATTGAGATTGCGATGGTGAAAAACCCAGAGGCGAGACAAGTCACATTCTCAAAGCGCCGAATGGGGTTATTCAAAAAAGCAAACGAGCTTGCCATCTTGTGTGGGGCCAAAATTGCTATTGTTGTATTCTCTCCCGGGAATAAACCTTACTCTTTTGGCCACCCTGGTGTTGATGCTATTGCGTCTAAATTTCTTCAACAAGAGCCCGATCTAAACGATGTCCTAGAAACCCCTTCCTCTAACATAGATGATCTGAACCAACAACATGAGAAGGTGATGGCTGATATAGCTGAGGCTGAAAAGGAAGATAAGGCCAATGAGGATATGCTAAAAGAATACAAATCGGCCCTACTCAGCCAGCCTAAGGAATGGAAAGATTCATTAATGGAGCTCAAGGATATGGTAAAATCACGTCTTCATGATTTAGATATATCAGATTCTATGATGCTCCTTGCAAAGGAGCCCGTGGTTGGAATTAAAAACCAAGCCACAGAAAAGAAAGGAAAATATTAA
Protein sequence

>Msa1323430

MGRRKIEIAMVKNPEARQVTFSKRRMGLFKKANELAILCGAKIAIVVFSPGNKPYSFGHPGVDAIASKFLQQEPDLNDVLETPSSNIDDLNQQHEKVMADIAEAEKEDKANEDMLKEYKSALLSQPKEWKDSLMELKDMVKSRLHDLDISDSMMLLAKEPVVGIKNQATEKKGKY*