AlfalfaGEDB | Msa1324650

M. sativa cultivar zhongmu-4 / Msa1324650

KEGG: -
GO: GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1324650	sp\|O81832\|Y4729_ARATH	33.387	620	313	19	4	532	13	623	1.98e-75	256

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1324650	A0A072VMI6	68.522	575	93	10	11	532	47	586	0.0	752

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1324650	MtrunA17_Chr8g0357801	56.818	572	142	16	1	476	17	579	0.0	561
Msa1324650	MtrunA17_Chr8g0357931	73.193	332	77	4	1	327	17	341	1.42e-172	501
Msa1324650	MtrunA17_Chr8g0357931	75.294	85	21	0	448	532	466	550	1.35e-29	123
Msa1324650	MtrunA17_Chr8g0357931	63.158	76	25	1	329	401	419	494	1.70e-20	95.1
Msa1324650	MtrunA17_Chr8g0358151	71.848	341	85	4	1	339	17	348	1.61e-172	492
Msa1324650	MtrunA17_Chr8g0357961	71.176	340	80	5	1	327	17	351	4.47e-166	491
Msa1324650	MtrunA17_Chr8g0357961	91.176	204	18	0	329	532	440	643	1.66e-124	384
Msa1324650	MtrunA17_Chr8g0357991	70.588	340	83	5	1	327	17	352	2.13e-165	489
Msa1324650	MtrunA17_Chr8g0357991	91.176	204	18	0	329	532	441	644	3.68e-124	382
Msa1324650	MtrunA17_Chr8g0358071	69.851	335	84	6	1	327	18	343	2.20e-163	483

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1324650	AT1G67520.1	65.909	176	60	0	357	532	404	579	1.05e-77	255
Msa1324650	AT1G67520.1	35.146	239	129	8	16	232	25	259	8.42e-27	114
Msa1324650	AT1G67520.5	65.909	176	60	0	357	532	242	417	3.11e-77	254
Msa1324650	AT1G67520.8	65.909	176	60	0	357	532	271	446	6.39e-77	254
Msa1324650	AT1G67520.7	65.909	176	60	0	357	532	271	446	6.39e-77	254
Msa1324650	AT1G67520.2	65.909	176	60	0	357	532	479	654	1.04e-76	254
Msa1324650	AT1G67520.2	38.500	200	105	7	13	198	22	217	1.25e-26	114
Msa1324650	AT4G27290.1	33.387	620	313	19	4	532	13	623	2.01e-76	256
Msa1324650	AT1G67520.4	65.909	176	60	0	357	532	436	611	1.29e-75	254
Msa1324650	AT1G67520.4	31.790	324	181	14	13	302	22	339	4.51e-28	119

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Find 104 sgRNAs with CRISPR-Local

Find 502 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATCCGGACAAGGAATTG+TGG	0.569989	8_4:+34938094	Msa1324650:CDS
AACGTTGCATTGTCCATGTT+TGG	0.363909	8_4:-34935451	None:intergenic
AACTTGCAGGGAACCTGGTG+AGG	0.606111	8_4:+34935405	Msa1324650:CDS
AAGAAAAGTAAAAGAAAGGA+AGG	0.457337	8_4:+34937473	Msa1324650:CDS
AAGATAGGAAGTTTATACGT+TGG	0.574754	8_4:+34935254	Msa1324650:CDS
AAGCGTTTCAACATAATAGA+AGG	0.410503	8_4:+34938471	Msa1324650:CDS
AAGTAAAAGAAAGGAAGGTA+AGG	0.405875	8_4:+34937478	Msa1324650:CDS
AAGTTAGGACAGGGAGGCTA+TGG	0.474937	8_4:+34937623	Msa1324650:CDS
AATATTTGAGAACATTCCAA+CGG	0.640176	8_4:+34935162	Msa1324650:CDS
AATTCTGTTGTTATTTACTA+CGG	0.478425	8_4:-34938643	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTCAACAAATTCACACAAA+TGG	+	chr8_4:34934905-34934924	Msa1324650:CDS	30.0%
ATCGAAAAACAGGACATAAT+TGG	+	chr8_4:34935002-34935021	Msa1324650:CDS	30.0%
AGCGGAAAACTTAAAAGTAA+TGG	+	chr8_4:34935139-34935158	Msa1324650:CDS	30.0%
AAGATAGGAAGTTTATACGT+TGG	+	chr8_4:34935254-34935273	Msa1324650:CDS	30.0%
TGGAGAAATTGTAATTGCAA+TGG	+	chr8_4:34935523-34935542	Msa1324650:intron	30.0%
CTGGATGTATATTCTATTCA+TGG	+	chr8_4:34935572-34935591	Msa1324650:intron	30.0%
GATTGACATCAACTATCTAA+TGG	-	chr8_4:34935765-34935784	None:intergenic	30.0%
TGAAGTTGAAGTATAGGATT+AGG	+	chr8_4:34937166-34937185	Msa1324650:intron	30.0%
GAAGTTGAAGTATAGGATTA+GGG	+	chr8_4:34937167-34937186	Msa1324650:intron	30.0%
TAGTCAATGCAGGTATAAAA+AGG	+	chr8_4:34937270-34937289	Msa1324650:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	34934551	34938653	34934551	ID=Msa1324650;Name=Msa1324650
chr8_4	mRNA	34934551	34938653	34934551	ID=Msa1324650-mRNA-1;Parent=Msa1324650;Name=Msa1324650-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0\|0.66\|0.5\|1\|1\|0.75\|4\|0\|532
chr8_4	exon	34934551	34935532	34934551	ID=Msa1324650-mRNA-1:exon:11089;Parent=Msa1324650-mRNA-1
chr8_4	exon	34937471	34937658	34937471	ID=Msa1324650-mRNA-1:exon:11090;Parent=Msa1324650-mRNA-1
chr8_4	exon	34938047	34938257	34938047	ID=Msa1324650-mRNA-1:exon:11091;Parent=Msa1324650-mRNA-1
chr8_4	exon	34938439	34938653	34938439	ID=Msa1324650-mRNA-1:exon:11092;Parent=Msa1324650-mRNA-1
chr8_4	CDS	34934551	34935532	34934551	ID=Msa1324650-mRNA-1:cds;Parent=Msa1324650-mRNA-1
chr8_4	CDS	34937471	34937658	34937471	ID=Msa1324650-mRNA-1:cds;Parent=Msa1324650-mRNA-1
chr8_4	CDS	34938047	34938257	34938047	ID=Msa1324650-mRNA-1:cds;Parent=Msa1324650-mRNA-1
chr8_4	CDS	34938439	34938653	34938439	ID=Msa1324650-mRNA-1:cds;Parent=Msa1324650-mRNA-1

Gene Sequence

>Msa1324650

TTGTGGTGGAGTACTACTTCTAGTATTTGTGTTAAAGCAACAAATGATAGTTTGAAGCCAGGTGATACAGTGAATTCCAATTCCTCGCTATGTTCTAAACAAGGCAAGTTTTGTCTTTCATTCTGGCCTAATGGCTACTTGATTATAAAATCTTTTAATGGCACAGATTCTGCGGAAGTGTGGATCTATAATAGAAATCAACCCGTAGACGTAAAATCAGCAGTTTTATCACTAGACTACACTGGAGTGCTCAAAATAGGATCTCAAAATAGGAAATTACCAATTATCATTTATTCTTCACCTCAACCCTCCAACAACACTGTCGCCACTATGTTAGACACGGGTAACTTTGTGCTTCAACAAATTCACACAAATGGGACAAAGAGTATATTATGGCAGAGTTTTGATTACCCCAGTGAAACTTTGATTCCAACGATGAAGTTGGGAGTTAATCGAAAAACAGGACATAATTGGTCATTGATTTCATGGTTGGCTCCTTCGCTACCAAATTCAGGTGGATTTAGTCTTGAATGGGAACCAAAAGAAGGTGAATTGAACATCAAGAAACGCGGCGAAGTGTATTGGAAAAGCGGAAAACTTAAAAGTAATGGAATATTTGAGAACATTCCAACGGAAGTTCAACGTATATATCAATACATTATTGTCTCTAACAAGGATGAAGATTCTTTTGCTTTCAAAGTTAAAGATAGGAAGTTTATACGTTGGTACATTTCTCTGAAGGGGAGGTTAGGTAGTACTGATGCTGGAAACAATGATAGTGGAGACATTGCTAATGCAGATAGGTGTTATGGATATAATAATGATGGAGGGTGCCAAAAATGGGAGGATATACCAACTTGCAGGGAACCTGGTGAGGTGTTTCAGAAAAGGGGGGGAAGACCAAACATGGACAATGCAACGTTTGAAGAGGATGATGTGACTTATAGTTACAGTGATTGCAAGATGAGATGTTGGAGAAATTATAAGAAAAGTAAAAGAAAGGAAGGTAAGGGCAACGATTTGGTGGAATCCTATGATATCAAAGATCTTGAAGATGACTTCAAGGGACATGATATAAAAGTTCTTAACTATACATCAATTTTGGAAGCAACTATGGACTTTTCACCTGTAAACAAGTTAGGACAGGGAGGCTATGGACCTGTTTATAAGGGAATCTTGGCAACAGGGCAAAAAGTTGCTGTAAAAAGACTTTCAAAAACATCCGGACAAGGAATTGTGGAATTCAAAAATGAATTGGTACTTATATGTGAACTTCAACACAGAAATCTTGTACAACTACTTGGTTGTTGCATTCATGAAGAAGAAAGGATTCTAATTTACGAATATATGCCAAACAAAAGCTTGGATTTCTACCTATTTGATTGTACGAAAAAGAATCTGCTAGATTGGAAGAAGCGTTTCAACATAATAGAAGGAATTGCTCAAGGATTGCTCTATCTTCATAAGTACTCAAGACTCAAAATCATTCACAGAGACTTGAAAGCTAGCAACATACTTCTTGATGAGAATATGAACCCAAAAATTTCTGATTTTGGTATGGCAAGAATGTTTACACAGCAGGAGTCCGTAGTAAAT

Protein sequence

>Msa1324650

LWWSTTSSICVKATNDSLKPGDTVNSNSSLCSKQGKFCLSFWPNGYLIIKSFNGTDSAEVWIYNRNQPVDVKSAVLSLDYTGVLKIGSQNRKLPIIIYSSPQPSNNTVATMLDTGNFVLQQIHTNGTKSILWQSFDYPSETLIPTMKLGVNRKTGHNWSLISWLAPSLPNSGGFSLEWEPKEGELNIKKRGEVYWKSGKLKSNGIFENIPTEVQRIYQYIIVSNKDEDSFAFKVKDRKFIRWYISLKGRLGSTDAGNNDSGDIANADRCYGYNNDGGCQKWEDIPTCREPGEVFQKRGGRPNMDNATFEEDDVTYSYSDCKMRCWRNYKKSKRKEGKGNDLVESYDIKDLEDDFKGHDIKVLNYTSILEATMDFSPVNKLGQGGYGPVYKGILATGQKVAVKRLSKTSGQGIVEFKNELVLICELQHRNLVQLLGCCIHEEERILIYEYMPNKSLDFYLFDCTKKNLLDWKKRFNIIEGIAQGLLYLHKYSRLKIIHRDLKASNILLDENMNPKISDFGMARMFTQQESVVN