Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325190 | KEH19246.1 | 84.615 | 182 | 20 | 1 | 1 | 182 | 1 | 174 | 1.09e-107 | 315 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325190 | A0A072TPV3 | 84.615 | 182 | 20 | 1 | 1 | 182 | 1 | 174 | 5.19e-108 | 315 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1325190 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325190 | MtrunA17_Chr8g0358731 | 84.615 | 182 | 20 | 1 | 1 | 182 | 1 | 174 | 9.99e-112 | 315 |
| Msa1325190 | MtrunA17_Chr8g0337271 | 36.416 | 173 | 90 | 5 | 1 | 153 | 1 | 173 | 7.13e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTATTCGCCAGTACATTTA+TGG | 0.169458 | 8_4:+37124715 | Msa1325190:CDS |
| TGAAAATCTTCATTTCTATA+TGG | 0.278484 | 8_4:-37124515 | None:intergenic |
| ACGCCAAGATGGTCGTCAAT+TGG | 0.390051 | 8_4:+37124655 | Msa1325190:CDS |
| TAGTGACAAGATGGCCTTCA+TGG | 0.398708 | 8_4:+37124787 | Msa1325190:CDS |
| TGCTGCACCTTGTCTGTCAT+AGG | 0.406280 | 8_4:-37124597 | None:intergenic |
| TGCAATAATGATTGTGAATA+CGG | 0.437294 | 8_4:+37124479 | Msa1325190:CDS |
| CTTGTCACTACGAGACATGA+TGG | 0.480223 | 8_4:-37124774 | None:intergenic |
| AATGATGACTTGCTTACCCT+TGG | 0.482188 | 8_4:-37124991 | None:intergenic |
| CATCTTGTAAATTTCCATGA+AGG | 0.485976 | 8_4:-37124801 | None:intergenic |
| GAGATCTTCCATGTCATCAC+TGG | 0.506949 | 8_4:-37125033 | None:intergenic |
| TCTCCAATTGACGACCATCT+TGG | 0.509670 | 8_4:-37124658 | None:intergenic |
| TCTCCACTTTCACTAGCGGT+TGG | 0.512325 | 8_4:-37124833 | None:intergenic |
| GGTATATAGTAATGCATCCA+AGG | 0.513039 | 8_4:+37124974 | Msa1325190:intron |
| AGATGGTCGTCAATTGGAGA+TGG | 0.520285 | 8_4:+37124661 | Msa1325190:CDS |
| CAAAGGAAACAAACATGCTA+GGG | 0.530048 | 8_4:+37124424 | Msa1325190:CDS |
| ACAAAAGGCCATAAATGTAC+TGG | 0.537910 | 8_4:-37124723 | None:intergenic |
| ATTCCAACCGCTAGTGAAAG+TGG | 0.538453 | 8_4:+37124830 | Msa1325190:CDS |
| ACAAAGGAAACAAACATGCT+AGG | 0.546583 | 8_4:+37124423 | Msa1325190:CDS |
| CTTAGCATAATACCTACAAA+AGG | 0.549427 | 8_4:-37124738 | None:intergenic |
| TTGTCACTACGAGACATGAT+GGG | 0.553748 | 8_4:-37124773 | None:intergenic |
| CATGTCTCGTAGTGACAAGA+TGG | 0.562759 | 8_4:+37124778 | Msa1325190:CDS |
| GCATGGCAACGACGCCAAGA+TGG | 0.563921 | 8_4:+37124644 | Msa1325190:CDS |
| TTCTCAGTCAAGGTCAAGCA+TGG | 0.575982 | 8_4:+37124627 | Msa1325190:CDS |
| ATGGAAATTTACAAGATGAA+CGG | 0.585442 | 8_4:+37124806 | Msa1325190:CDS |
| TTCTGAAGCCAGTGATGACA+TGG | 0.592729 | 8_4:+37125025 | Msa1325190:CDS |
| GATGGTCGTCAATTGGAGAT+GGG | 0.600387 | 8_4:+37124662 | Msa1325190:CDS |
| GGATTCTCCACTTTCACTAG+CGG | 0.605182 | 8_4:-37124837 | None:intergenic |
| CTACGAGACATGATGGGGAA+TGG | 0.605683 | 8_4:-37124767 | None:intergenic |
| GCAAACTCGATTCTCAGTCA+AGG | 0.622555 | 8_4:+37124617 | Msa1325190:CDS |
| GAATAGTGCTTTCATCTCAA+AGG | 0.627573 | 8_4:-37124699 | None:intergenic |
| CTAGTGAAAGTGGAGAATCC+AGG | 0.632875 | 8_4:+37124840 | Msa1325190:CDS |
| GTAAATCCAAACATGAACAA+AGG | 0.634906 | 8_4:+37124407 | None:intergenic |
| GTATATAGTAATGCATCCAA+GGG | 0.647018 | 8_4:+37124975 | Msa1325190:intron |
| TGTCACTACGAGACATGATG+GGG | 0.670159 | 8_4:-37124772 | None:intergenic |
| CATGAGTATGATCGAAAGTG+CGG | 0.672466 | 8_4:+37124571 | Msa1325190:CDS |
| GCGGAAACCTATGACAGACA+AGG | 0.718948 | 8_4:+37124590 | Msa1325190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAATAGAAAAAATAATATG+TGG | - | chr8_4:37124925-37124944 | None:intergenic | 15.0% |
| !! | TGAAAATCTTCATTTCTATA+TGG | - | chr8_4:37124518-37124537 | None:intergenic | 20.0% |
| !!! | ACACTCTTTATATCAAATTT+TGG | + | chr8_4:37124953-37124972 | Msa1325190:intron | 20.0% |
| ! | TGCAATAATGATTGTGAATA+CGG | + | chr8_4:37124479-37124498 | Msa1325190:CDS | 25.0% |
| ! | ATGGAAATTTACAAGATGAA+CGG | + | chr8_4:37124806-37124825 | Msa1325190:CDS | 25.0% |
| CTTAGCATAATACCTACAAA+AGG | - | chr8_4:37124741-37124760 | None:intergenic | 30.0% | |
| CATCTTGTAAATTTCCATGA+AGG | - | chr8_4:37124804-37124823 | None:intergenic | 30.0% | |
| AGTACAATATAGAGATTACC+TGG | - | chr8_4:37124861-37124880 | None:intergenic | 30.0% | |
| GTATATAGTAATGCATCCAA+GGG | + | chr8_4:37124975-37124994 | Msa1325190:intron | 30.0% | |
| ! | ATCTTGCATTTTCATTTGGA+GGG | - | chr8_4:37125058-37125077 | None:intergenic | 30.0% |
| ! | TCAGATCTTGCATTTTCATT+TGG | - | chr8_4:37125062-37125081 | None:intergenic | 30.0% |
| ACAAAGGAAACAAACATGCT+AGG | + | chr8_4:37124423-37124442 | Msa1325190:CDS | 35.0% | |
| CAAAGGAAACAAACATGCTA+GGG | + | chr8_4:37124424-37124443 | Msa1325190:CDS | 35.0% | |
| GAATAGTGCTTTCATCTCAA+AGG | - | chr8_4:37124702-37124721 | None:intergenic | 35.0% | |
| ACTATTCGCCAGTACATTTA+TGG | + | chr8_4:37124715-37124734 | Msa1325190:CDS | 35.0% | |
| ACAAAAGGCCATAAATGTAC+TGG | - | chr8_4:37124726-37124745 | None:intergenic | 35.0% | |
| ! | GTACATTTATGGCCTTTTGT+AGG | + | chr8_4:37124726-37124745 | Msa1325190:CDS | 35.0% |
| GGTATATAGTAATGCATCCA+AGG | + | chr8_4:37124974-37124993 | Msa1325190:intron | 35.0% | |
| ! | GATCTTGCATTTTCATTTGG+AGG | - | chr8_4:37125059-37125078 | None:intergenic | 35.0% |
| CATGAGTATGATCGAAAGTG+CGG | + | chr8_4:37124571-37124590 | Msa1325190:CDS | 40.0% | |
| TTGTCACTACGAGACATGAT+GGG | - | chr8_4:37124776-37124795 | None:intergenic | 40.0% | |
| !! | AATGATGACTTGCTTACCCT+TGG | - | chr8_4:37124994-37125013 | None:intergenic | 40.0% |
| GCAAACTCGATTCTCAGTCA+AGG | + | chr8_4:37124617-37124636 | Msa1325190:CDS | 45.0% | |
| TTCTCAGTCAAGGTCAAGCA+TGG | + | chr8_4:37124627-37124646 | Msa1325190:CDS | 45.0% | |
| TCTCCAATTGACGACCATCT+TGG | - | chr8_4:37124661-37124680 | None:intergenic | 45.0% | |
| AGATGGTCGTCAATTGGAGA+TGG | + | chr8_4:37124661-37124680 | Msa1325190:CDS | 45.0% | |
| GATGGTCGTCAATTGGAGAT+GGG | + | chr8_4:37124662-37124681 | Msa1325190:CDS | 45.0% | |
| TGTCACTACGAGACATGATG+GGG | - | chr8_4:37124775-37124794 | None:intergenic | 45.0% | |
| CTTGTCACTACGAGACATGA+TGG | - | chr8_4:37124777-37124796 | None:intergenic | 45.0% | |
| CATGTCTCGTAGTGACAAGA+TGG | + | chr8_4:37124778-37124797 | Msa1325190:CDS | 45.0% | |
| TAGTGACAAGATGGCCTTCA+TGG | + | chr8_4:37124787-37124806 | Msa1325190:CDS | 45.0% | |
| ATTCCAACCGCTAGTGAAAG+TGG | + | chr8_4:37124830-37124849 | Msa1325190:CDS | 45.0% | |
| GGATTCTCCACTTTCACTAG+CGG | - | chr8_4:37124840-37124859 | None:intergenic | 45.0% | |
| CTAGTGAAAGTGGAGAATCC+AGG | + | chr8_4:37124840-37124859 | Msa1325190:CDS | 45.0% | |
| !! | TTCTGAAGCCAGTGATGACA+TGG | + | chr8_4:37125025-37125044 | Msa1325190:CDS | 45.0% |
| GAGATCTTCCATGTCATCAC+TGG | - | chr8_4:37125036-37125055 | None:intergenic | 45.0% | |
| GCGGAAACCTATGACAGACA+AGG | + | chr8_4:37124590-37124609 | Msa1325190:CDS | 50.0% | |
| TGCTGCACCTTGTCTGTCAT+AGG | - | chr8_4:37124600-37124619 | None:intergenic | 50.0% | |
| ACGCCAAGATGGTCGTCAAT+TGG | + | chr8_4:37124655-37124674 | Msa1325190:CDS | 50.0% | |
| ! | CTACGAGACATGATGGGGAA+TGG | - | chr8_4:37124770-37124789 | None:intergenic | 50.0% |
| TCTCCACTTTCACTAGCGGT+TGG | - | chr8_4:37124836-37124855 | None:intergenic | 50.0% | |
| GCATGGCAACGACGCCAAGA+TGG | + | chr8_4:37124644-37124663 | Msa1325190:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 37124419 | 37125088 | 37124419 | ID=Msa1325190;Name=Msa1325190 |
| chr8_4 | mRNA | 37124419 | 37125088 | 37124419 | ID=Msa1325190-mRNA-1;Parent=Msa1325190;Name=Msa1325190-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0|1|1|1|2|0|182 |
| chr8_4 | exon | 37124419 | 37124861 | 37124419 | ID=Msa1325190-mRNA-1:exon:11376;Parent=Msa1325190-mRNA-1 |
| chr8_4 | exon | 37124983 | 37125088 | 37124983 | ID=Msa1325190-mRNA-1:exon:11377;Parent=Msa1325190-mRNA-1 |
| chr8_4 | CDS | 37124419 | 37124861 | 37124419 | ID=Msa1325190-mRNA-1:cds;Parent=Msa1325190-mRNA-1 |
| chr8_4 | CDS | 37124983 | 37125088 | 37124983 | ID=Msa1325190-mRNA-1:cds;Parent=Msa1325190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
