Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325200 | A0A072TPV3 | 91.837 | 98 | 8 | 0 | 1 | 98 | 1 | 98 | 1.72e-59 | 189 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1325200 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325200 | MtrunA17_Chr8g0358731 | 91.837 | 98 | 8 | 0 | 1 | 98 | 1 | 98 | 3.32e-63 | 189 |
| Msa1325200 | MtrunA17_Chr8g0337271 | 39.796 | 98 | 55 | 1 | 1 | 94 | 1 | 98 | 5.44e-22 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325200 | AT1G36000.1 | 34.615 | 78 | 51 | 0 | 2 | 79 | 1 | 78 | 1.70e-11 | 56.6 |
Find 19 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGTGCTCTTAGAAGTTT+AGG | 0.233419 | 8_4:+37127866 | Msa1325200:CDS |
| TGCTGCACTTTGTCTGTCAT+AGG | 0.406280 | 8_4:-37127927 | None:intergenic |
| ATGCCAAGATGGTCGTCAAT+TGG | 0.432997 | 8_4:+37127985 | Msa1325200:CDS |
| TGCAATAATGATTGTGAATA+CGG | 0.437294 | 8_4:+37127809 | Msa1325200:CDS |
| CTTGGCATTGTTGCCATGCT+CGG | 0.452495 | 8_4:-37127970 | None:intergenic |
| TCTCCAATTGACGACCATCT+TGG | 0.501049 | 8_4:-37127988 | None:intergenic |
| ATCTAACATCATGAGTATGA+TGG | 0.517001 | 8_4:+37127892 | Msa1325200:CDS |
| AGATGGTCGTCAATTGGAGA+TGG | 0.520285 | 8_4:+37127991 | Msa1325200:CDS |
| TGAAAATCTTCATTTCTATG+TGG | 0.529192 | 8_4:-37127845 | None:intergenic |
| TGGAAGGAAACAAACATGCT+AGG | 0.531725 | 8_4:+37127753 | Msa1325200:CDS |
| TTCTCAGTCAAGGCCGAGCA+TGG | 0.544627 | 8_4:+37127957 | Msa1325200:CDS |
| GGAAGGAAACAAACATGCTA+GGG | 0.550449 | 8_4:+37127754 | Msa1325200:CDS |
| GCATGGCAACAATGCCAAGA+TGG | 0.568439 | 8_4:+37127974 | Msa1325200:CDS |
| AAGAGATATCTTCCTTTGAG+AGG | 0.575332 | 8_4:+37128017 | Msa1325200:CDS |
| GTAAATCCAAACATGATGGA+AGG | 0.580485 | 8_4:+37127737 | None:intergenic |
| GAATAGTCATTTCCTCTCAA+AGG | 0.595250 | 8_4:-37128029 | None:intergenic |
| GATGGTCGTCAATTGGAGAT+GGG | 0.600387 | 8_4:+37127992 | Msa1325200:CDS |
| GCAAACTCGATTCTCAGTCA+AGG | 0.608229 | 8_4:+37127947 | Msa1325200:CDS |
| CATGAGTATGATGGAAAGTG+TGG | 0.625793 | 8_4:+37127901 | Msa1325200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGCAATAATGATTGTGAATA+CGG | + | chr8_4:37127809-37127828 | Msa1325200:CDS | 25.0% |
| ! | TGAAAATCTTCATTTCTATG+TGG | - | chr8_4:37127848-37127867 | None:intergenic | 25.0% |
| ATCTAACATCATGAGTATGA+TGG | + | chr8_4:37127892-37127911 | Msa1325200:CDS | 30.0% | |
| ! | CAAAGTGCTCTTAGAAGTTT+AGG | + | chr8_4:37127866-37127885 | Msa1325200:CDS | 35.0% |
| AAGAGATATCTTCCTTTGAG+AGG | + | chr8_4:37128017-37128036 | Msa1325200:CDS | 35.0% | |
| TGGAAGGAAACAAACATGCT+AGG | + | chr8_4:37127753-37127772 | Msa1325200:CDS | 40.0% | |
| GGAAGGAAACAAACATGCTA+GGG | + | chr8_4:37127754-37127773 | Msa1325200:CDS | 40.0% | |
| ! | CATGAGTATGATGGAAAGTG+TGG | + | chr8_4:37127901-37127920 | Msa1325200:CDS | 40.0% |
| TGCTGCACTTTGTCTGTCAT+AGG | - | chr8_4:37127930-37127949 | None:intergenic | 45.0% | |
| GCAAACTCGATTCTCAGTCA+AGG | + | chr8_4:37127947-37127966 | Msa1325200:CDS | 45.0% | |
| ATGCCAAGATGGTCGTCAAT+TGG | + | chr8_4:37127985-37128004 | Msa1325200:CDS | 45.0% | |
| TCTCCAATTGACGACCATCT+TGG | - | chr8_4:37127991-37128010 | None:intergenic | 45.0% | |
| AGATGGTCGTCAATTGGAGA+TGG | + | chr8_4:37127991-37128010 | Msa1325200:CDS | 45.0% | |
| GATGGTCGTCAATTGGAGAT+GGG | + | chr8_4:37127992-37128011 | Msa1325200:CDS | 45.0% | |
| CTTGGCATTGTTGCCATGCT+CGG | - | chr8_4:37127973-37127992 | None:intergenic | 50.0% | |
| GCATGGCAACAATGCCAAGA+TGG | + | chr8_4:37127974-37127993 | Msa1325200:CDS | 50.0% | |
| TTCTCAGTCAAGGCCGAGCA+TGG | + | chr8_4:37127957-37127976 | Msa1325200:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 37127749 | 37128045 | 37127749 | ID=Msa1325200;Name=Msa1325200 |
| chr8_4 | mRNA | 37127749 | 37128045 | 37127749 | ID=Msa1325200-mRNA-1;Parent=Msa1325200;Name=Msa1325200-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|98 |
| chr8_4 | exon | 37127749 | 37128045 | 37127749 | ID=Msa1325200-mRNA-1:exon:11378;Parent=Msa1325200-mRNA-1 |
| chr8_4 | CDS | 37127749 | 37128045 | 37127749 | ID=Msa1325200-mRNA-1:cds;Parent=Msa1325200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
