Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325210 | A0A072TPV3 | 87.912 | 182 | 13 | 2 | 1 | 181 | 1 | 174 | 1.31e-110 | 322 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1325210 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1325210 | MtrunA17_Chr8g0358731 | 87.912 | 182 | 13 | 2 | 1 | 181 | 1 | 174 | 2.51e-114 | 322 |
| Msa1325210 | MtrunA17_Chr8g0337271 | 36.022 | 186 | 92 | 6 | 9 | 167 | 10 | 195 | 2.17e-28 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTATTCGCCAGTACATTTA+TGG | 0.169458 | 8_4:+37152198 | Msa1325210:CDS |
| CAAAGTGCTCTTAGAAGTTT+AGG | 0.233419 | 8_4:+37152019 | Msa1325210:CDS |
| TCGGATCTTGCATATTCATT+TGG | 0.326460 | 8_4:-37152542 | None:intergenic |
| ACGCCAAGATGGTCGTCAAT+TGG | 0.390051 | 8_4:+37152138 | Msa1325210:CDS |
| TAGTTACAAGATGACCTTCA+TGG | 0.400415 | 8_4:+37152270 | Msa1325210:CDS |
| TGCTGCACCTTGTCTGTCAT+AGG | 0.406280 | 8_4:-37152080 | None:intergenic |
| AGAATCACTTTGCTCAATGA+TGG | 0.410197 | 8_4:-37152489 | None:intergenic |
| AATGATGGCTTGCTTACCCT+TGG | 0.435794 | 8_4:-37152474 | None:intergenic |
| TGCAATAATGATTGTGAATA+CGG | 0.437294 | 8_4:+37151962 | Msa1325210:CDS |
| CTTGTAACTACGAGACATGA+TGG | 0.480223 | 8_4:-37152257 | None:intergenic |
| CATCTTGTAAATTTCCATGA+AGG | 0.485976 | 8_4:-37152284 | None:intergenic |
| GATCTTGCATATTCATTTGG+AGG | 0.493998 | 8_4:-37152539 | None:intergenic |
| TCTCCACTTTCACTAGCGGT+TGG | 0.507765 | 8_4:-37152316 | None:intergenic |
| TCTCCAATTGACGACCATCT+TGG | 0.509670 | 8_4:-37152141 | None:intergenic |
| ATCTAACATCATGAGTATGA+TGG | 0.517001 | 8_4:+37152045 | Msa1325210:CDS |
| GGTATACAGCAATGCATCCA+AGG | 0.519872 | 8_4:+37152457 | Msa1325210:intron |
| AGATGGTCGTCAATTGGAGA+TGG | 0.520285 | 8_4:+37152144 | Msa1325210:CDS |
| ACTAACCACAATCTACTCTT+CGG | 0.522583 | 8_4:-37152561 | None:intergenic |
| TGAAAATCTTCATTTCTATG+TGG | 0.529192 | 8_4:-37151998 | None:intergenic |
| GTAAATACAAACATGAATGA+AGG | 0.530220 | 8_4:+37151893 | None:intergenic |
| ATCTTGCATATTCATTTGGA+GGG | 0.532675 | 8_4:-37152538 | None:intergenic |
| ACAAAAGGCCATAAATGTAC+TGG | 0.537910 | 8_4:-37152206 | None:intergenic |
| ATTCCAACCGCTAGTGAAAG+TGG | 0.538453 | 8_4:+37152313 | Msa1325210:CDS |
| CTTAGCATAATACCTACAAA+AGG | 0.549427 | 8_4:-37152221 | None:intergenic |
| TGAATGAAGGAAACAAACAT+AGG | 0.551981 | 8_4:+37151906 | Msa1325210:CDS |
| TTGTAACTACGAGACATGAT+GGG | 0.552157 | 8_4:-37152256 | None:intergenic |
| GCATGGCAACGACGCCAAGA+TGG | 0.574695 | 8_4:+37152127 | Msa1325210:CDS |
| AAGAGATATCTTCCTTTGAG+AGG | 0.575332 | 8_4:+37152170 | Msa1325210:CDS |
| CTAGTGAAAGTGGAGAATCT+AGG | 0.575408 | 8_4:+37152323 | Msa1325210:CDS |
| TTCTCAGTCAAGGTCGAGCA+TGG | 0.575982 | 8_4:+37152110 | Msa1325210:CDS |
| ATGGAAATTTACAAGATGAA+CGG | 0.585442 | 8_4:+37152289 | Msa1325210:CDS |
| GAATGAAGGAAACAAACATA+GGG | 0.596750 | 8_4:+37151907 | Msa1325210:CDS |
| GATGGTCGTCAATTGGAGAT+GGG | 0.600387 | 8_4:+37152145 | Msa1325210:CDS |
| CTACGAGACATGATGGGGAA+TGG | 0.605683 | 8_4:-37152250 | None:intergenic |
| TTCTGAAGACAGTGATGACA+TGG | 0.616959 | 8_4:+37152508 | Msa1325210:CDS |
| GAATAGTGCTTTCCTCTCAA+AGG | 0.618448 | 8_4:-37152182 | None:intergenic |
| GCAAACTCGATTCTCAGTCA+AGG | 0.622555 | 8_4:+37152100 | Msa1325210:CDS |
| AGATTCTCCACTTTCACTAG+CGG | 0.625760 | 8_4:-37152320 | None:intergenic |
| CATGAGTATGATGGAAAGTG+TGG | 0.625793 | 8_4:+37152054 | Msa1325210:CDS |
| GTATACAGCAATGCATCCAA+GGG | 0.652464 | 8_4:+37152458 | Msa1325210:intron |
| TGTAACTACGAGACATGATG+GGG | 0.692794 | 8_4:-37152255 | None:intergenic |
| GTGGAAACCTATGACAGACA+AGG | 0.716652 | 8_4:+37152073 | Msa1325210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACACTCTTTATATCAAATTT+TGG | + | chr8_4:37152436-37152455 | Msa1325210:intron | 20.0% |
| ! | TGCAATAATGATTGTGAATA+CGG | + | chr8_4:37151962-37151981 | Msa1325210:CDS | 25.0% |
| ! | TGAAAATCTTCATTTCTATG+TGG | - | chr8_4:37152001-37152020 | None:intergenic | 25.0% |
| ! | ATGGAAATTTACAAGATGAA+CGG | + | chr8_4:37152289-37152308 | Msa1325210:CDS | 25.0% |
| !! | TGATATAAAGAGTGTGTTAT+AGG | - | chr8_4:37152431-37152450 | None:intergenic | 25.0% |
| TGAATGAAGGAAACAAACAT+AGG | + | chr8_4:37151906-37151925 | Msa1325210:CDS | 30.0% | |
| GAATGAAGGAAACAAACATA+GGG | + | chr8_4:37151907-37151926 | Msa1325210:CDS | 30.0% | |
| ATCTAACATCATGAGTATGA+TGG | + | chr8_4:37152045-37152064 | Msa1325210:CDS | 30.0% | |
| CTTAGCATAATACCTACAAA+AGG | - | chr8_4:37152224-37152243 | None:intergenic | 30.0% | |
| CATCTTGTAAATTTCCATGA+AGG | - | chr8_4:37152287-37152306 | None:intergenic | 30.0% | |
| ATCTTGCATATTCATTTGGA+GGG | - | chr8_4:37152541-37152560 | None:intergenic | 30.0% | |
| ! | CAAAGTGCTCTTAGAAGTTT+AGG | + | chr8_4:37152019-37152038 | Msa1325210:CDS | 35.0% |
| AAGAGATATCTTCCTTTGAG+AGG | + | chr8_4:37152170-37152189 | Msa1325210:CDS | 35.0% | |
| ACTATTCGCCAGTACATTTA+TGG | + | chr8_4:37152198-37152217 | Msa1325210:CDS | 35.0% | |
| ACAAAAGGCCATAAATGTAC+TGG | - | chr8_4:37152209-37152228 | None:intergenic | 35.0% | |
| ! | GTACATTTATGGCCTTTTGT+AGG | + | chr8_4:37152209-37152228 | Msa1325210:CDS | 35.0% |
| TTGTAACTACGAGACATGAT+GGG | - | chr8_4:37152259-37152278 | None:intergenic | 35.0% | |
| TAGTTACAAGATGACCTTCA+TGG | + | chr8_4:37152270-37152289 | Msa1325210:CDS | 35.0% | |
| AGAATCACTTTGCTCAATGA+TGG | - | chr8_4:37152492-37152511 | None:intergenic | 35.0% | |
| GATCTTGCATATTCATTTGG+AGG | - | chr8_4:37152542-37152561 | None:intergenic | 35.0% | |
| TCGGATCTTGCATATTCATT+TGG | - | chr8_4:37152545-37152564 | None:intergenic | 35.0% | |
| ! | CATGAGTATGATGGAAAGTG+TGG | + | chr8_4:37152054-37152073 | Msa1325210:CDS | 40.0% |
| GAATAGTGCTTTCCTCTCAA+AGG | - | chr8_4:37152185-37152204 | None:intergenic | 40.0% | |
| TGTAACTACGAGACATGATG+GGG | - | chr8_4:37152258-37152277 | None:intergenic | 40.0% | |
| CTTGTAACTACGAGACATGA+TGG | - | chr8_4:37152260-37152279 | None:intergenic | 40.0% | |
| AGATTCTCCACTTTCACTAG+CGG | - | chr8_4:37152323-37152342 | None:intergenic | 40.0% | |
| CTAGTGAAAGTGGAGAATCT+AGG | + | chr8_4:37152323-37152342 | Msa1325210:CDS | 40.0% | |
| GTATACAGCAATGCATCCAA+GGG | + | chr8_4:37152458-37152477 | Msa1325210:intron | 40.0% | |
| ! | TTCTGAAGACAGTGATGACA+TGG | + | chr8_4:37152508-37152527 | Msa1325210:CDS | 40.0% |
| GTGGAAACCTATGACAGACA+AGG | + | chr8_4:37152073-37152092 | Msa1325210:CDS | 45.0% | |
| GCAAACTCGATTCTCAGTCA+AGG | + | chr8_4:37152100-37152119 | Msa1325210:CDS | 45.0% | |
| TCTCCAATTGACGACCATCT+TGG | - | chr8_4:37152144-37152163 | None:intergenic | 45.0% | |
| AGATGGTCGTCAATTGGAGA+TGG | + | chr8_4:37152144-37152163 | Msa1325210:CDS | 45.0% | |
| GATGGTCGTCAATTGGAGAT+GGG | + | chr8_4:37152145-37152164 | Msa1325210:CDS | 45.0% | |
| ATTCCAACCGCTAGTGAAAG+TGG | + | chr8_4:37152313-37152332 | Msa1325210:CDS | 45.0% | |
| GGTATACAGCAATGCATCCA+AGG | + | chr8_4:37152457-37152476 | Msa1325210:intron | 45.0% | |
| !! | AATGATGGCTTGCTTACCCT+TGG | - | chr8_4:37152477-37152496 | None:intergenic | 45.0% |
| TGCTGCACCTTGTCTGTCAT+AGG | - | chr8_4:37152083-37152102 | None:intergenic | 50.0% | |
| TTCTCAGTCAAGGTCGAGCA+TGG | + | chr8_4:37152110-37152129 | Msa1325210:CDS | 50.0% | |
| ACGCCAAGATGGTCGTCAAT+TGG | + | chr8_4:37152138-37152157 | Msa1325210:CDS | 50.0% | |
| ! | CTACGAGACATGATGGGGAA+TGG | - | chr8_4:37152253-37152272 | None:intergenic | 50.0% |
| TCTCCACTTTCACTAGCGGT+TGG | - | chr8_4:37152319-37152338 | None:intergenic | 50.0% | |
| GCATGGCAACGACGCCAAGA+TGG | + | chr8_4:37152127-37152146 | Msa1325210:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 37151905 | 37152571 | 37151905 | ID=Msa1325210;Name=Msa1325210 |
| chr8_4 | mRNA | 37151905 | 37152571 | 37151905 | ID=Msa1325210-mRNA-1;Parent=Msa1325210;Name=Msa1325210-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|2|0|181 |
| chr8_4 | exon | 37151905 | 37152344 | 37151905 | ID=Msa1325210-mRNA-1:exon:11374;Parent=Msa1325210-mRNA-1 |
| chr8_4 | exon | 37152466 | 37152571 | 37152466 | ID=Msa1325210-mRNA-1:exon:11375;Parent=Msa1325210-mRNA-1 |
| chr8_4 | CDS | 37151905 | 37152344 | 37151905 | ID=Msa1325210-mRNA-1:cds;Parent=Msa1325210-mRNA-1 |
| chr8_4 | CDS | 37152466 | 37152571 | 37152466 | ID=Msa1325210-mRNA-1:cds;Parent=Msa1325210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
