Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1326100 | sp|F4JZT3|ARR24_ARATH | 56.780 | 118 | 49 | 1 | 10 | 127 | 16 | 131 | 8.15e-37 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1326100 | G7LG58 | 94.444 | 144 | 8 | 0 | 1 | 144 | 1 | 144 | 8.03e-95 | 279 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1326100 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1326100 | MtrunA17_Chr8g0364781 | 94.444 | 144 | 8 | 0 | 1 | 144 | 1 | 144 | 1.55e-98 | 279 |
| Msa1326100 | MtrunA17_Chr7g0262351 | 58.400 | 125 | 49 | 1 | 5 | 129 | 15 | 136 | 1.09e-42 | 138 |
| Msa1326100 | MtrunA17_Chr7g0262671 | 62.821 | 78 | 28 | 1 | 1 | 78 | 1 | 77 | 2.08e-27 | 97.8 |
| Msa1326100 | MtrunA17_Chr7g0262681 | 65.672 | 67 | 18 | 2 | 66 | 132 | 1 | 62 | 5.70e-20 | 78.2 |
| Msa1326100 | MtrunA17_Chr6g0472261 | 54.795 | 73 | 33 | 0 | 6 | 78 | 4 | 76 | 8.16e-20 | 77.8 |
| Msa1326100 | MtrunA17_Chr6g0451941 | 34.259 | 108 | 69 | 1 | 12 | 119 | 33 | 138 | 3.03e-14 | 65.5 |
| Msa1326100 | MtrunA17_Chr8g0371601 | 31.206 | 141 | 74 | 4 | 12 | 131 | 1029 | 1167 | 3.79e-13 | 65.5 |
| Msa1326100 | MtrunA17_Chr8g0392301 | 31.159 | 138 | 67 | 4 | 15 | 132 | 864 | 993 | 6.17e-13 | 65.1 |
| Msa1326100 | MtrunA17_Chr1g0194731 | 38.017 | 121 | 55 | 5 | 15 | 122 | 331 | 444 | 7.43e-12 | 61.6 |
| Msa1326100 | MtrunA17_Chr5g0447641 | 41.791 | 67 | 39 | 0 | 15 | 81 | 1077 | 1143 | 2.40e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1326100 | AT5G26594.1 | 56.780 | 118 | 49 | 1 | 10 | 127 | 16 | 131 | 8.29e-38 | 125 |
| Msa1326100 | AT3G04280.1 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 2.94e-31 | 109 |
| Msa1326100 | AT3G04280.2 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 2.94e-31 | 109 |
| Msa1326100 | AT3G04280.3 | 51.786 | 112 | 52 | 2 | 12 | 122 | 22 | 132 | 2.94e-31 | 109 |
| Msa1326100 | AT2G17820.1 | 27.950 | 161 | 78 | 4 | 15 | 139 | 1047 | 1205 | 5.29e-13 | 65.5 |
| Msa1326100 | AT2G01830.2 | 30.579 | 121 | 66 | 3 | 15 | 122 | 948 | 1063 | 1.18e-11 | 61.6 |
| Msa1326100 | AT2G01830.1 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 1.19e-11 | 61.6 |
| Msa1326100 | AT2G01830.4 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 1.19e-11 | 61.6 |
| Msa1326100 | AT2G01830.6 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 1.19e-11 | 61.6 |
| Msa1326100 | AT2G01830.3 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 1.19e-11 | 61.6 |
| Msa1326100 | AT2G01830.5 | 30.579 | 121 | 66 | 3 | 15 | 122 | 937 | 1052 | 1.19e-11 | 61.6 |
Find 28 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATAGTGATAAAATTTA+TGG | 0.245050 | 8_4:-40014237 | Msa1326100:CDS |
| TGGTGTATCCTCACACTCTA+TGG | 0.342570 | 8_4:-40014267 | Msa1326100:CDS |
| TGACCTAATCTTGATGGATA+AGG | 0.347722 | 8_4:-40014544 | Msa1326100:CDS |
| ACGTCATTTGATGAATTCTT+CGG | 0.351042 | 8_4:+40014646 | None:intergenic |
| CACAACAAGTGCTGTTAACT+TGG | 0.355344 | 8_4:+40014686 | None:intergenic |
| CGTCATTTGATGAATTCTTC+GGG | 0.366651 | 8_4:+40014647 | None:intergenic |
| AACAAAGCAACTAAGGTTGA+TGG | 0.449452 | 8_4:-40014309 | Msa1326100:CDS |
| GTGATAAAATTTATGGAAGC+AGG | 0.456780 | 8_4:-40014230 | Msa1326100:CDS |
| AATTCCATTCTTCATGCCGT+TGG | 0.460947 | 8_4:-40014164 | Msa1326100:CDS |
| TATCCTTATCCATCAAGATT+AGG | 0.462938 | 8_4:+40014541 | None:intergenic |
| TCTTGAGAAACCATTGGATA+AGG | 0.467024 | 8_4:-40014195 | Msa1326100:CDS |
| GGGATATCTAGCACTATTGT+TGG | 0.469032 | 8_4:-40014287 | Msa1326100:CDS |
| TCATTCCATCTTGTGAGGAA+TGG | 0.470437 | 8_4:+40014118 | None:intergenic |
| TATAGGCAACAAAGCAACTA+AGG | 0.474253 | 8_4:-40014316 | Msa1326100:intron |
| CAAATGACGTTGAGTAACTT+AGG | 0.479404 | 8_4:-40014633 | Msa1326100:CDS |
| ACCTCAGCTCCATTCATCTC+AGG | 0.491878 | 8_4:+40014516 | None:intergenic |
| AAGGATATGCCTGAGATGAA+TGG | 0.499108 | 8_4:-40014525 | Msa1326100:CDS |
| ACAAAGCAACTAAGGTTGAT+GGG | 0.523464 | 8_4:-40014308 | Msa1326100:CDS |
| CGAATATCTTGAGAAACCAT+TGG | 0.533361 | 8_4:-40014201 | Msa1326100:CDS |
| CTCCTCTTCCATAGAGTGTG+AGG | 0.555361 | 8_4:+40014259 | None:intergenic |
| AAATTCCATTCCTCACAAGA+TGG | 0.561625 | 8_4:-40014123 | Msa1326100:CDS |
| ATCCTCACACTCTATGGAAG+AGG | 0.570487 | 8_4:-40014261 | Msa1326100:CDS |
| TTTGTTCATTCCATCTTGTG+AGG | 0.573958 | 8_4:+40014113 | None:intergenic |
| CAAGTTAACAGCACTTGTTG+TGG | 0.584291 | 8_4:-40014685 | Msa1326100:CDS |
| CTCACACTCTATGGAAGAGG+AGG | 0.604784 | 8_4:-40014258 | Msa1326100:CDS |
| CAAAGCAACTAAGGTTGATG+GGG | 0.617733 | 8_4:-40014307 | Msa1326100:CDS |
| GCAGTGGAAATTCATCAGCA+TGG | 0.649906 | 8_4:-40014576 | Msa1326100:CDS |
| GCCTGAGATGAATGGAGCTG+AGG | 0.680215 | 8_4:-40014517 | Msa1326100:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATGTGTTTCTTATAT+AGG | - | chr8_4:40014499-40014518 | Msa1326100:intron | 15.0% |
| !!! | TTTTTCTTTTATTTTTCCAA+CGG | + | chr8_4:40014687-40014706 | None:intergenic | 15.0% |
| !! | GGAAATAGTGATAAAATTTA+TGG | - | chr8_4:40014595-40014614 | Msa1326100:CDS | 20.0% |
| !!! | ATTTTTTGACCTAATCTTGA+TGG | - | chr8_4:40014282-40014301 | Msa1326100:CDS | 25.0% |
| !!! | TTGGATGTAAAGTTTTGAGA+TGG | + | chr8_4:40014130-40014149 | None:intergenic | 30.0% |
| ACGTCATTTGATGAATTCTT+CGG | + | chr8_4:40014189-40014208 | None:intergenic | 30.0% | |
| AACAACACTTGTGTGAAAAA+CGG | - | chr8_4:40014226-40014245 | Msa1326100:CDS | 30.0% | |
| TATCCTTATCCATCAAGATT+AGG | + | chr8_4:40014294-40014313 | None:intergenic | 30.0% | |
| GAGTTCATTCTTGCTTATTT+AGG | - | chr8_4:40014397-40014416 | Msa1326100:intron | 30.0% | |
| GTGATAAAATTTATGGAAGC+AGG | - | chr8_4:40014602-40014621 | Msa1326100:CDS | 30.0% | |
| CGTCATTTGATGAATTCTTC+GGG | + | chr8_4:40014188-40014207 | None:intergenic | 35.0% | |
| CAAATGACGTTGAGTAACTT+AGG | - | chr8_4:40014199-40014218 | Msa1326100:CDS | 35.0% | |
| ! | TGACCTAATCTTGATGGATA+AGG | - | chr8_4:40014288-40014307 | Msa1326100:CDS | 35.0% |
| TATAGGCAACAAAGCAACTA+AGG | - | chr8_4:40014516-40014535 | Msa1326100:intron | 35.0% | |
| AACAAAGCAACTAAGGTTGA+TGG | - | chr8_4:40014523-40014542 | Msa1326100:CDS | 35.0% | |
| ! | ACAAAGCAACTAAGGTTGAT+GGG | - | chr8_4:40014524-40014543 | Msa1326100:CDS | 35.0% |
| CGAATATCTTGAGAAACCAT+TGG | - | chr8_4:40014631-40014650 | Msa1326100:CDS | 35.0% | |
| TCTTGAGAAACCATTGGATA+AGG | - | chr8_4:40014637-40014656 | Msa1326100:CDS | 35.0% | |
| ! | TTGAGTTTTGCCTTATCCAA+TGG | + | chr8_4:40014650-40014669 | None:intergenic | 35.0% |
| AAATTCCATTCCTCACAAGA+TGG | - | chr8_4:40014709-40014728 | Msa1326100:CDS | 35.0% | |
| ! | CACAACAAGTGCTGTTAACT+TGG | + | chr8_4:40014149-40014168 | None:intergenic | 40.0% |
| ! | CAAGTTAACAGCACTTGTTG+TGG | - | chr8_4:40014147-40014166 | Msa1326100:CDS | 40.0% |
| GAAAAACGGAAAAGAAGCAG+TGG | - | chr8_4:40014240-40014259 | Msa1326100:CDS | 40.0% | |
| AAGGATATGCCTGAGATGAA+TGG | - | chr8_4:40014307-40014326 | Msa1326100:CDS | 40.0% | |
| CATTCTTGCTTATTTAGGCG+TGG | - | chr8_4:40014402-40014421 | Msa1326100:intron | 40.0% | |
| !! | CAAAGCAACTAAGGTTGATG+GGG | - | chr8_4:40014525-40014544 | Msa1326100:CDS | 40.0% |
| GGGATATCTAGCACTATTGT+TGG | - | chr8_4:40014545-40014564 | Msa1326100:CDS | 40.0% | |
| AATTCCATTCTTCATGCCGT+TGG | - | chr8_4:40014668-40014687 | Msa1326100:CDS | 40.0% | |
| ! | TTTTCCAACGGCATGAAGAA+TGG | + | chr8_4:40014675-40014694 | None:intergenic | 40.0% |
| TCATTCCATCTTGTGAGGAA+TGG | + | chr8_4:40014717-40014736 | None:intergenic | 40.0% | |
| GCAGTGGAAATTCATCAGCA+TGG | - | chr8_4:40014256-40014275 | Msa1326100:CDS | 45.0% | |
| TGGTGTATCCTCACACTCTA+TGG | - | chr8_4:40014565-40014584 | Msa1326100:CDS | 45.0% | |
| ATCCTCACACTCTATGGAAG+AGG | - | chr8_4:40014571-40014590 | Msa1326100:CDS | 45.0% | |
| !! | ATATATAAATGTAAAATTTA+GGG | + | chr8_4:40014361-40014380 | None:intergenic | 5.0% |
| !! | TATATATAAATGTAAAATTT+AGG | + | chr8_4:40014362-40014381 | None:intergenic | 5.0% |
| ACCTCAGCTCCATTCATCTC+AGG | + | chr8_4:40014319-40014338 | None:intergenic | 50.0% | |
| CTCCTCTTCCATAGAGTGTG+AGG | + | chr8_4:40014576-40014595 | None:intergenic | 50.0% | |
| CTCACACTCTATGGAAGAGG+AGG | - | chr8_4:40014574-40014593 | Msa1326100:CDS | 50.0% | |
| GCCTGAGATGAATGGAGCTG+AGG | - | chr8_4:40014315-40014334 | Msa1326100:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 40014118 | 40014736 | 40014118 | ID=Msa1326100;Name=Msa1326100 |
| chr8_4 | mRNA | 40014118 | 40014736 | 40014118 | ID=Msa1326100-mRNA-1;Parent=Msa1326100;Name=Msa1326100-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|144 |
| chr8_4 | exon | 40014518 | 40014736 | 40014518 | ID=Msa1326100-mRNA-1:exon:11879;Parent=Msa1326100-mRNA-1 |
| chr8_4 | exon | 40014118 | 40014333 | 40014118 | ID=Msa1326100-mRNA-1:exon:11878;Parent=Msa1326100-mRNA-1 |
| chr8_4 | CDS | 40014518 | 40014736 | 40014518 | ID=Msa1326100-mRNA-1:cds;Parent=Msa1326100-mRNA-1 |
| chr8_4 | CDS | 40014118 | 40014333 | 40014118 | ID=Msa1326100-mRNA-1:cds;Parent=Msa1326100-mRNA-1 |
| Gene Sequence |
| Protein sequence |
