AlfalfaGEDB | Msa1326740

M. sativa cultivar zhongmu-4 / Msa1326740

KEGG: K14498
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0097159 GO:0140096 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1326740	sp\|P43291\|SRK2A_ARATH	65.385	130	24	1	1	109	180	309	8.02e-56	180

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1326740	A0A1S2YF16	80.147	136	6	1	1	115	180	315	1.97e-69	221

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1326740	MtrunA17_Chr8g0385281	80.147	136	6	1	1	115	181	316	1.12e-72	219
Msa1326740	MtrunA17_Chr3g0131061	71.852	135	17	1	1	114	180	314	6.86e-66	202
Msa1326740	MtrunA17_Chr2g0277501	63.704	135	28	1	1	114	180	314	7.18e-57	179
Msa1326740	MtrunA17_Chr5g0399911	52.419	124	37	2	1	102	195	318	2.70e-36	126
Msa1326740	MtrunA17_Chr4g0055451	50.000	136	42	3	1	113	181	313	6.93e-36	125
Msa1326740	MtrunA17_Chr8g0381821	48.333	120	39	2	1	99	180	297	7.45e-32	114
Msa1326740	MtrunA17_Chr5g0426521	49.593	123	38	2	1	102	180	299	7.61e-32	114
Msa1326740	MtrunA17_Chr4g0055431	50.407	123	35	3	1	100	187	306	9.33e-32	114
Msa1326740	MtrunA17_Chr4g0045371	48.780	123	41	2	1	102	199	320	2.91e-31	113
Msa1326740	MtrunA17_Chr3g0123791	46.400	125	44	2	1	102	181	305	3.07e-31	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1326740	AT1G10940.2	65.385	130	24	1	1	109	188	317	7.51e-57	180
Msa1326740	AT1G10940.1	65.385	130	24	1	1	109	180	309	8.16e-57	180
Msa1326740	AT1G60940.1	64.444	135	27	1	1	114	180	314	1.21e-56	179
Msa1326740	AT1G60940.2	64.444	135	27	1	1	114	180	314	1.21e-56	179
Msa1326740	AT5G63650.1	67.424	132	22	1	2	112	181	312	4.49e-56	177
Msa1326740	AT5G08590.2	65.414	133	24	2	2	113	105	236	5.35e-54	170
Msa1326740	AT5G08590.1	65.414	133	24	2	2	113	181	312	2.78e-53	170
Msa1326740	AT2G23030.1	61.194	134	29	2	3	113	182	315	1.25e-46	153
Msa1326740	AT4G33950.2	47.619	126	40	3	1	103	197	319	2.58e-31	113
Msa1326740	AT4G33950.1	44.444	135	49	3	1	112	197	328	3.99e-31	113

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Find 31 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTTGGGTTGCCTCTC+TGG	0.343755	8_4:+41351750	None:intergenic
ACACTTTGAATAGAAAAGCT+TGG	0.460081	8_4:+41351737	None:intergenic
ACCCTTTATGTCATGCTAGT+TGG	0.433752	8_4:-41352430	Msa1326740:CDS
ACCGGCCTAGATACCGGAGG+AGG	0.562102	8_4:+41351677	None:intergenic
ACCTCCTCCGGTATCTAGGC+CGG	0.548787	8_4:-41351678	Msa1326740:CDS
AGAGGCAAGAGAACCTCCTC+CGG	0.578941	8_4:-41351690	Msa1326740:CDS
AGCATATCCCTTTGAGGATC+GGG	0.410059	8_4:-41352407	Msa1326740:CDS
AGGGTCATCCCGATCCTCAA+AGG	0.547475	8_4:+41352399	None:intergenic
AGTTGGAGCATATCCCTTTG+AGG	0.541202	8_4:-41352413	Msa1326740:CDS
CAACAAATATACGAGATAGG+AGG	0.614585	8_4:+41352198	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTGCAAAAGAAGATATGAAA+TGG	+	chr8_4:41351835-41351854	None:intergenic	30.0%
GAGATATGTGAACATAATCA+GGG	+	chr8_4:41351884-41351903	None:intergenic	30.0%
TGAGATATGTGAACATAATC+AGG	+	chr8_4:41351885-41351904	None:intergenic	30.0%
TAGCAACAAATATACGAGAT+AGG	+	chr8_4:41351923-41351942	None:intergenic	30.0%
TCATCAAAACATACCCTTAA+TGG	+	chr8_4:41351948-41351967	None:intergenic	30.0%
TAAGAACTTGTGAGATTGTT+TGG	+	chr8_4:41352163-41352182	None:intergenic	30.0%
AACAATCTCACAAGTTCTTA+CGG	-	chr8_4:41352163-41352182	Msa1326740:intron	30.0%
CGATAAACAATGGATCTATT+AGG	+	chr8_4:41352215-41352234	None:intergenic	30.0%
CACTTTGAATAGAAAAGCTT+GGG	+	chr8_4:41352380-41352399	None:intergenic	30.0%
ACACTTTGAATAGAAAAGCT+TGG	+	chr8_4:41352381-41352400	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	41351658	41352479	41351658	ID=Msa1326740;Name=Msa1326740
chr8_4	mRNA	41351658	41352479	41351658	ID=Msa1326740-mRNA-1;Parent=Msa1326740;Name=Msa1326740-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0\|0\|1\|0.5\|0.66\|3\|0\|115
chr8_4	exon	41352375	41352479	41352375	ID=Msa1326740-mRNA-1:exon:12161;Parent=Msa1326740-mRNA-1
chr8_4	exon	41352202	41352282	41352202	ID=Msa1326740-mRNA-1:exon:12160;Parent=Msa1326740-mRNA-1
chr8_4	exon	41351658	41351816	41351658	ID=Msa1326740-mRNA-1:exon:12159;Parent=Msa1326740-mRNA-1
chr8_4	CDS	41352375	41352479	41352375	ID=Msa1326740-mRNA-1:cds;Parent=Msa1326740-mRNA-1
chr8_4	CDS	41352202	41352282	41352202	ID=Msa1326740-mRNA-1:cds;Parent=Msa1326740-mRNA-1
chr8_4	CDS	41351658	41351816	41351658	ID=Msa1326740-mRNA-1:cds;Parent=Msa1326740-mRNA-1

Gene Sequence	>Msa1326740 TTGGCTGATGTGTGGTCCTGTGGAGTGACCCTTTATGTCATGCTAGTTGGAGCATATCCCTTTGAGGATCGGGATGACCCTAGAAATTTTATGAAAACAATCCAGCTCATAATGGCTCTTCAATACAAAATCCCTGATTATGTTCACATATCTCAAGATTGCAAAAGCCTCCTATCTCGTATATTTAATCTTCCAAGGGAGCTAATTGAATCAGCTCAAGCTGCATATTACCAGAGAGGCAACCCAAGCTTTTCTATTCAAAGTGTGGACGAGATAATGAAAATTGTGGGAGAGGCAAGAGAACCTCCTCCGGTATCTAGGCCGGTCAAAGGTTTTGGCTGGGAA
Protein sequence	>Msa1326740 LADVWSCGVTLYVMLVGAYPFEDRDDPRNFMKTIQLIMALQYKIPDYVHISQDCKSLLSRIFNLPRELIESAQAAYYQRGNPSFSIQSVDEIMKIVGEAREPPPVSRPVKGFGWE