Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1327840 | XP_024629447.1 | 93.056 | 144 | 0 | 1 | 44 | 177 | 1 | 144 | 8.33e-87 | 278 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1327840 | A0A396GJD5 | 93.056 | 144 | 0 | 1 | 44 | 177 | 1 | 144 | 3.98e-87 | 278 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1327840 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1327840 | MtrunA17_Chr8g0363841 | 93.056 | 144 | 0 | 1 | 44 | 177 | 1 | 144 | 7.66e-91 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGTTACCAAGTTGATAT+TGG | 0.311387 | 8_4:-45305993 | Msa1327840:CDS |
| CTGCAGCTATCGTGGGTCAC+TGG | 0.323331 | 8_4:+45306627 | None:intergenic |
| CAAGAGCATGCTTTCAAAAC+AGG | 0.323625 | 8_4:-45306208 | Msa1327840:CDS |
| AACTTTCCACACTCCTTCCT+TGG | 0.367826 | 8_4:+45306009 | None:intergenic |
| AGAAGACCGCATAAAGTCTT+TGG | 0.373881 | 8_4:-45305897 | None:intergenic |
| GAGCCTAGAGATATTTATCT+TGG | 0.393295 | 8_4:-45306271 | Msa1327840:CDS |
| CTAGGCTCATCAGTTTCAAT+TGG | 0.394540 | 8_4:+45306286 | None:intergenic |
| TTATAAGCCACTTCACCATC+AGG | 0.415225 | 8_4:+45306082 | None:intergenic |
| TGCAGCTATCGTGGGTCACT+GGG | 0.455907 | 8_4:+45306628 | None:intergenic |
| AACAAATATCTTTGCTGAAG+TGG | 0.482406 | 8_4:+45305950 | None:intergenic |
| AAGAACAAATTGCTTAGCTA+TGG | 0.488702 | 8_4:-45306047 | Msa1327840:CDS |
| CGGCAATGGAGGAAGAAAGA+GGG | 0.508249 | 8_4:+45306598 | None:intergenic |
| TGACCAAGATAAATATCTCT+AGG | 0.529714 | 8_4:+45306268 | None:intergenic |
| GGGAACTTCTGCAGCTATCG+TGG | 0.529768 | 8_4:+45306619 | None:intergenic |
| AGATTTAATGTGACCAAGGA+AGG | 0.533919 | 8_4:-45306022 | Msa1327840:CDS |
| AATGTATAACTTACTGTATG+AGG | 0.537016 | 8_4:+45306532 | None:intergenic |
| ACGGCAATGGAGGAAGAAAG+AGG | 0.588216 | 8_4:+45306597 | None:intergenic |
| TCGACGAACTACGACGGCAA+TGG | 0.588386 | 8_4:+45306584 | None:intergenic |
| ACGAACTACGACGGCAATGG+AGG | 0.588501 | 8_4:+45306587 | None:intergenic |
| GCTATCGTGGGTCACTGGGT+TGG | 0.593020 | 8_4:+45306632 | None:intergenic |
| GGAACTTCTGCAGCTATCGT+GGG | 0.600988 | 8_4:+45306620 | None:intergenic |
| CAATGAACCTGATGGTGAAG+TGG | 0.607386 | 8_4:-45306089 | Msa1327840:CDS |
| GTATAACTTACTGTATGAGG+AGG | 0.608910 | 8_4:+45306535 | None:intergenic |
| ATTGTGATCCAATATCAACT+TGG | 0.629329 | 8_4:+45305985 | None:intergenic |
| ATGTGACCAAGGAAGGAGTG+TGG | 0.636883 | 8_4:-45306015 | Msa1327840:CDS |
| GGCAATGGAGGAAGAAAGAG+GGG | 0.637595 | 8_4:+45306599 | None:intergenic |
| TATAACTTACTGTATGAGGA+GGG | 0.642871 | 8_4:+45306536 | None:intergenic |
| GGTCAGATTTAATGTGACCA+AGG | 0.650278 | 8_4:-45306026 | Msa1327840:CDS |
| CGACGATCGACGAACTACGA+CGG | 0.672070 | 8_4:+45306578 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAAAAGATAATATTAAAA+AGG | + | chr8_4:45306039-45306058 | None:intergenic | 10.0% |
| !!! | CTTTTTAATATTATCTTTTT+TGG | - | chr8_4:45306037-45306056 | Msa1327840:CDS | 10.0% |
| !! | CAAAAAAGATAATATTAAAA+AGG | + | chr8_4:45306039-45306058 | None:intergenic | 10.0% |
| !!! | CTTTTTAATATTATCTTTTT+TGG | - | chr8_4:45306037-45306056 | Msa1327840:CDS | 10.0% |
| !! | GAATTCATCATATTTATTTA+TGG | + | chr8_4:45306064-45306083 | None:intergenic | 15.0% |
| !!! | ATTTTTATGTAGAGAAAATA+TGG | - | chr8_4:45306217-45306236 | Msa1327840:CDS | 15.0% |
| !! | GAATTCATCATATTTATTTA+TGG | + | chr8_4:45306064-45306083 | None:intergenic | 15.0% |
| !!! | ATTTTTATGTAGAGAAAATA+TGG | - | chr8_4:45306217-45306236 | Msa1327840:CDS | 15.0% |
| !!! | GTTTTGAATTTAATTTCACA+TGG | + | chr8_4:45306086-45306105 | None:intergenic | 20.0% |
| !!! | ATTGTTTTTAAATCCTTGTT+TGG | + | chr8_4:45306438-45306457 | None:intergenic | 20.0% |
| !!! | GTTTTGAATTTAATTTCACA+TGG | + | chr8_4:45306086-45306105 | None:intergenic | 20.0% |
| !!! | ATTGTTTTTAAATCCTTGTT+TGG | + | chr8_4:45306438-45306457 | None:intergenic | 20.0% |
| ! | AATGTATAACTTACTGTATG+AGG | + | chr8_4:45306014-45306033 | None:intergenic | 25.0% |
| ! | AATTGATAAATTAGGGGAAA+GGG | + | chr8_4:45306123-45306142 | None:intergenic | 25.0% |
| ! | AGCAGTGAATTGATAAATTA+GGG | + | chr8_4:45306130-45306149 | None:intergenic | 25.0% |
| ! | TTTAAAAACAATGAACCTGA+TGG | - | chr8_4:45306446-45306465 | Msa1327840:intron | 25.0% |
| ! | AATGTATAACTTACTGTATG+AGG | + | chr8_4:45306014-45306033 | None:intergenic | 25.0% |
| ! | AATTGATAAATTAGGGGAAA+GGG | + | chr8_4:45306123-45306142 | None:intergenic | 25.0% |
| ! | AGCAGTGAATTGATAAATTA+GGG | + | chr8_4:45306130-45306149 | None:intergenic | 25.0% |
| ! | TTTAAAAACAATGAACCTGA+TGG | - | chr8_4:45306446-45306465 | Msa1327840:intron | 25.0% |
| TATAACTTACTGTATGAGGA+GGG | + | chr8_4:45306010-45306029 | None:intergenic | 30.0% | |
| GAATTGATAAATTAGGGGAA+AGG | + | chr8_4:45306124-45306143 | None:intergenic | 30.0% | |
| GCAGTGAATTGATAAATTAG+GGG | + | chr8_4:45306129-45306148 | None:intergenic | 30.0% | |
| GAGCAGTGAATTGATAAATT+AGG | + | chr8_4:45306131-45306150 | None:intergenic | 30.0% | |
| TGACCAAGATAAATATCTCT+AGG | + | chr8_4:45306278-45306297 | None:intergenic | 30.0% | |
| !! | AACAGGTTTTAGTGTAAGAA+AGG | - | chr8_4:45306352-45306371 | Msa1327840:intron | 30.0% |
| !! | ACAGGTTTTAGTGTAAGAAA+GGG | - | chr8_4:45306353-45306372 | Msa1327840:intron | 30.0% |
| GAAAAAGAAAACACGTTTGA+AGG | - | chr8_4:45306400-45306419 | Msa1327840:intron | 30.0% | |
| ! | GACTTTTATTGTTCCAAACA+AGG | - | chr8_4:45306422-45306441 | Msa1327840:intron | 30.0% |
| AAGAACAAATTGCTTAGCTA+TGG | - | chr8_4:45306496-45306515 | Msa1327840:intron | 30.0% | |
| !! | GAAAGTTACCAAGTTGATAT+TGG | - | chr8_4:45306550-45306569 | Msa1327840:CDS | 30.0% |
| ATTGTGATCCAATATCAACT+TGG | + | chr8_4:45306561-45306580 | None:intergenic | 30.0% | |
| AACAAATATCTTTGCTGAAG+TGG | + | chr8_4:45306596-45306615 | None:intergenic | 30.0% | |
| TATAACTTACTGTATGAGGA+GGG | + | chr8_4:45306010-45306029 | None:intergenic | 30.0% | |
| GAATTGATAAATTAGGGGAA+AGG | + | chr8_4:45306124-45306143 | None:intergenic | 30.0% | |
| GCAGTGAATTGATAAATTAG+GGG | + | chr8_4:45306129-45306148 | None:intergenic | 30.0% | |
| GAGCAGTGAATTGATAAATT+AGG | + | chr8_4:45306131-45306150 | None:intergenic | 30.0% | |
| TGACCAAGATAAATATCTCT+AGG | + | chr8_4:45306278-45306297 | None:intergenic | 30.0% | |
| !! | AACAGGTTTTAGTGTAAGAA+AGG | - | chr8_4:45306352-45306371 | Msa1327840:intron | 30.0% |
| !! | ACAGGTTTTAGTGTAAGAAA+GGG | - | chr8_4:45306353-45306372 | Msa1327840:intron | 30.0% |
| GAAAAAGAAAACACGTTTGA+AGG | - | chr8_4:45306400-45306419 | Msa1327840:intron | 30.0% | |
| ! | GACTTTTATTGTTCCAAACA+AGG | - | chr8_4:45306422-45306441 | Msa1327840:intron | 30.0% |
| AAGAACAAATTGCTTAGCTA+TGG | - | chr8_4:45306496-45306515 | Msa1327840:intron | 30.0% | |
| !! | GAAAGTTACCAAGTTGATAT+TGG | - | chr8_4:45306550-45306569 | Msa1327840:CDS | 30.0% |
| ATTGTGATCCAATATCAACT+TGG | + | chr8_4:45306561-45306580 | None:intergenic | 30.0% | |
| AACAAATATCTTTGCTGAAG+TGG | + | chr8_4:45306596-45306615 | None:intergenic | 30.0% | |
| GTATAACTTACTGTATGAGG+AGG | + | chr8_4:45306011-45306030 | None:intergenic | 35.0% | |
| ACCAGTAGTAGATTTCAGTT+AGG | - | chr8_4:45306164-45306183 | Msa1327840:CDS | 35.0% | |
| CCAGTAGTAGATTTCAGTTA+GGG | - | chr8_4:45306165-45306184 | Msa1327840:CDS | 35.0% | |
| GAGCCTAGAGATATTTATCT+TGG | - | chr8_4:45306272-45306291 | Msa1327840:CDS | 35.0% | |
| !! | CAGGTTTTAGTGTAAGAAAG+GGG | - | chr8_4:45306354-45306373 | Msa1327840:intron | 35.0% |
| ! | TTTTAGTGTAAGAAAGGGGA+AGG | - | chr8_4:45306358-45306377 | Msa1327840:intron | 35.0% |
| AGATTTAATGTGACCAAGGA+AGG | - | chr8_4:45306521-45306540 | Msa1327840:intron | 35.0% | |
| GTATAACTTACTGTATGAGG+AGG | + | chr8_4:45306011-45306030 | None:intergenic | 35.0% | |
| ACCAGTAGTAGATTTCAGTT+AGG | - | chr8_4:45306164-45306183 | Msa1327840:CDS | 35.0% | |
| CCAGTAGTAGATTTCAGTTA+GGG | - | chr8_4:45306165-45306184 | Msa1327840:CDS | 35.0% | |
| GAGCCTAGAGATATTTATCT+TGG | - | chr8_4:45306272-45306291 | Msa1327840:CDS | 35.0% | |
| !! | CAGGTTTTAGTGTAAGAAAG+GGG | - | chr8_4:45306354-45306373 | Msa1327840:intron | 35.0% |
| ! | TTTTAGTGTAAGAAAGGGGA+AGG | - | chr8_4:45306358-45306377 | Msa1327840:intron | 35.0% |
| AGATTTAATGTGACCAAGGA+AGG | - | chr8_4:45306521-45306540 | Msa1327840:intron | 35.0% | |
| CCCTAACTGAAATCTACTAC+TGG | + | chr8_4:45306168-45306187 | None:intergenic | 40.0% | |
| AGTAGATTTCAGTTAGGGCA+TGG | - | chr8_4:45306170-45306189 | Msa1327840:CDS | 40.0% | |
| GTAGATTTCAGTTAGGGCAT+GGG | - | chr8_4:45306171-45306190 | Msa1327840:CDS | 40.0% | |
| CTAGGCTCATCAGTTTCAAT+TGG | + | chr8_4:45306260-45306279 | None:intergenic | 40.0% | |
| !!! | CTGTTTTGAAAGCATGCTCT+TGG | + | chr8_4:45306337-45306356 | None:intergenic | 40.0% |
| ! | CAAGAGCATGCTTTCAAAAC+AGG | - | chr8_4:45306335-45306354 | Msa1327840:intron | 40.0% |
| TTATAAGCCACTTCACCATC+AGG | + | chr8_4:45306464-45306483 | None:intergenic | 40.0% | |
| GGTCAGATTTAATGTGACCA+AGG | - | chr8_4:45306517-45306536 | Msa1327840:intron | 40.0% | |
| CCCTAACTGAAATCTACTAC+TGG | + | chr8_4:45306168-45306187 | None:intergenic | 40.0% | |
| AGTAGATTTCAGTTAGGGCA+TGG | - | chr8_4:45306170-45306189 | Msa1327840:CDS | 40.0% | |
| GTAGATTTCAGTTAGGGCAT+GGG | - | chr8_4:45306171-45306190 | Msa1327840:CDS | 40.0% | |
| CTAGGCTCATCAGTTTCAAT+TGG | + | chr8_4:45306260-45306279 | None:intergenic | 40.0% | |
| !!! | CTGTTTTGAAAGCATGCTCT+TGG | + | chr8_4:45306337-45306356 | None:intergenic | 40.0% |
| ! | CAAGAGCATGCTTTCAAAAC+AGG | - | chr8_4:45306335-45306354 | Msa1327840:intron | 40.0% |
| TTATAAGCCACTTCACCATC+AGG | + | chr8_4:45306464-45306483 | None:intergenic | 40.0% | |
| GGTCAGATTTAATGTGACCA+AGG | - | chr8_4:45306517-45306536 | Msa1327840:intron | 40.0% | |
| ! | CAATGAACCTGATGGTGAAG+TGG | - | chr8_4:45306454-45306473 | Msa1327840:intron | 45.0% |
| AACTTTCCACACTCCTTCCT+TGG | + | chr8_4:45306537-45306556 | None:intergenic | 45.0% | |
| ! | CAATGAACCTGATGGTGAAG+TGG | - | chr8_4:45306454-45306473 | Msa1327840:intron | 45.0% |
| AACTTTCCACACTCCTTCCT+TGG | + | chr8_4:45306537-45306556 | None:intergenic | 45.0% | |
| !! | AAAAAAGATAATATTAAAAA+GGG | + | chr8_4:45306038-45306057 | None:intergenic | 5.0% |
| !! | AAAAAAGATAATATTAAAAA+GGG | + | chr8_4:45306038-45306057 | None:intergenic | 5.0% |
| GGAACTTCTGCAGCTATCGT+GGG | + | chr8_4:45305926-45305945 | None:intergenic | 50.0% | |
| GGCAATGGAGGAAGAAAGAG+GGG | + | chr8_4:45305947-45305966 | None:intergenic | 50.0% | |
| CGGCAATGGAGGAAGAAAGA+GGG | + | chr8_4:45305948-45305967 | None:intergenic | 50.0% | |
| ACGGCAATGGAGGAAGAAAG+AGG | + | chr8_4:45305949-45305968 | None:intergenic | 50.0% | |
| ATGTGACCAAGGAAGGAGTG+TGG | - | chr8_4:45306528-45306547 | Msa1327840:intron | 50.0% | |
| GGAACTTCTGCAGCTATCGT+GGG | + | chr8_4:45305926-45305945 | None:intergenic | 50.0% | |
| GGCAATGGAGGAAGAAAGAG+GGG | + | chr8_4:45305947-45305966 | None:intergenic | 50.0% | |
| CGGCAATGGAGGAAGAAAGA+GGG | + | chr8_4:45305948-45305967 | None:intergenic | 50.0% | |
| ACGGCAATGGAGGAAGAAAG+AGG | + | chr8_4:45305949-45305968 | None:intergenic | 50.0% | |
| ATGTGACCAAGGAAGGAGTG+TGG | - | chr8_4:45306528-45306547 | Msa1327840:intron | 50.0% | |
| TGCAGCTATCGTGGGTCACT+GGG | + | chr8_4:45305918-45305937 | None:intergenic | 55.0% | |
| GGGAACTTCTGCAGCTATCG+TGG | + | chr8_4:45305927-45305946 | None:intergenic | 55.0% | |
| ACGAACTACGACGGCAATGG+AGG | + | chr8_4:45305959-45305978 | None:intergenic | 55.0% | |
| TCGACGAACTACGACGGCAA+TGG | + | chr8_4:45305962-45305981 | None:intergenic | 55.0% | |
| CGACGATCGACGAACTACGA+CGG | + | chr8_4:45305968-45305987 | None:intergenic | 55.0% | |
| TGCAGCTATCGTGGGTCACT+GGG | + | chr8_4:45305918-45305937 | None:intergenic | 55.0% | |
| GGGAACTTCTGCAGCTATCG+TGG | + | chr8_4:45305927-45305946 | None:intergenic | 55.0% | |
| ACGAACTACGACGGCAATGG+AGG | + | chr8_4:45305959-45305978 | None:intergenic | 55.0% | |
| TCGACGAACTACGACGGCAA+TGG | + | chr8_4:45305962-45305981 | None:intergenic | 55.0% | |
| CGACGATCGACGAACTACGA+CGG | + | chr8_4:45305968-45305987 | None:intergenic | 55.0% | |
| GCTATCGTGGGTCACTGGGT+TGG | + | chr8_4:45305914-45305933 | None:intergenic | 60.0% | |
| CTGCAGCTATCGTGGGTCAC+TGG | + | chr8_4:45305919-45305938 | None:intergenic | 60.0% | |
| GCTATCGTGGGTCACTGGGT+TGG | + | chr8_4:45305914-45305933 | None:intergenic | 60.0% | |
| CTGCAGCTATCGTGGGTCAC+TGG | + | chr8_4:45305919-45305938 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 45305898 | 45306667 | 45305898 | ID=Msa1327840;Name=Msa1327840 |
| chr8_4 | mRNA | 45305898 | 45306667 | 45305898 | ID=Msa1327840-mRNA-1;Parent=Msa1327840;Name=Msa1327840-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0.5|0|1|0.5|0.33|3|0|177 |
| chr8_4 | exon | 45305898 | 45306121 | 45305898 | ID=Msa1327840-mRNA-1:exon:12717;Parent=Msa1327840-mRNA-1 |
| chr8_4 | exon | 45306152 | 45306336 | 45306152 | ID=Msa1327840-mRNA-1:exon:12716;Parent=Msa1327840-mRNA-1 |
| chr8_4 | exon | 45306546 | 45306667 | 45306546 | ID=Msa1327840-mRNA-1:exon:12715;Parent=Msa1327840-mRNA-1 |
| chr8_4 | CDS | 45306546 | 45306667 | 45306546 | ID=Msa1327840-mRNA-1:cds;Parent=Msa1327840-mRNA-1 |
| chr8_4 | CDS | 45306152 | 45306336 | 45306152 | ID=Msa1327840-mRNA-1:cds;Parent=Msa1327840-mRNA-1 |
| chr8_4 | CDS | 45305898 | 45306121 | 45305898 | ID=Msa1327840-mRNA-1:cds;Parent=Msa1327840-mRNA-1 |
| Gene Sequence |
| Protein sequence |
