AlfalfaGEDB | Msa1328400

M. sativa cultivar zhongmu-4 / Msa1328400

KEGG: -
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046982 GO:0046983 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF05142.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328400	sp\|Q9SD40\|SRS1_ARATH	44.275	393	141	11	1	338	1	370	1.92e-79	249

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328400	A0A072TSM8	95.858	338	7	2	1	338	1	331	0.0	663

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TFs/TRs:

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Gene ID	Type	Classification
Msa1328400	TF	SRS

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328400	MtrunA17_Chr8g0372461	95.858	338	7	2	1	338	1	331	0.0	663
Msa1328400	MtrunA17_Chr5g0404781	52.342	363	108	13	1	338	1	323	2.90e-91	275
Msa1328400	MtrunA17_Chr3g0142171	43.064	346	126	16	1	333	1	288	2.47e-63	203
Msa1328400	MtrunA17_Chr1g0155791	51.915	235	75	7	126	338	120	338	4.86e-58	191
Msa1328400	MtrunA17_Chr5g0441921	50.323	155	54	4	128	259	124	278	8.16e-41	145
Msa1328400	MtrunA17_Chr4g0035591	47.887	142	65	2	124	265	16	148	2.84e-40	140
Msa1328400	MtrunA17_Chr3g0082511	39.869	153	69	1	128	257	134	286	1.49e-33	126
Msa1328400	MtrunA17_Chr8g0353111	39.884	173	73	3	115	257	111	282	4.25e-33	125
Msa1328400	MtrunA17_Chr0c01g0489181	56.410	39	17	0	124	162	63	101	6.08e-14	67.4
Msa1328400	MtrunA17_Chr2g0281741	61.538	39	15	0	124	162	146	184	4.30e-13	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328400	AT3G51060.1	44.275	393	141	11	1	338	1	370	1.95e-80	249
Msa1328400	AT5G66350.1	46.113	373	124	15	1	338	1	331	4.49e-74	232
Msa1328400	AT5G66350.3	45.856	362	121	14	1	327	1	322	7.69e-72	226
Msa1328400	AT5G66350.2	45.983	361	120	14	1	326	1	321	1.19e-71	226
Msa1328400	AT1G19790.2	42.781	374	147	16	1	337	1	344	3.34e-69	220
Msa1328400	AT1G19790.1	42.781	374	147	16	1	337	1	344	3.34e-69	220
Msa1328400	AT1G75520.1	41.755	376	151	15	1	338	1	346	1.07e-62	203
Msa1328400	AT4G36260.1	39.889	361	150	13	1	336	1	319	2.15e-54	181
Msa1328400	AT2G21400.2	47.701	174	83	3	125	294	7	176	6.29e-49	162
Msa1328400	AT2G21400.4	47.701	174	83	3	125	294	6	175	7.56e-49	162

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Find 114 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGAGATTATTATATGA+AGG	0.508828	8_4:+46226575	Msa1328400:three_prime_UTR
AAACAAAATTGAGAAGAAAG+AGG	0.504247	8_4:+46226405	Msa1328400:three_prime_UTR
AAACTCATGTTAAAAGCACT+TGG	0.502171	8_4:+46225068	Msa1328400:CDS
AAAGGAATTCTCTATGATTT+TGG	0.200894	8_4:+46226135	Msa1328400:CDS
AACAAACGTTGAAGGAAGGA+AGG	0.563270	8_4:+46224149	Msa1328400:five_prime_UTR
AACAACAGTGGTATCAGAGT+CGG	0.622301	8_4:-46226277	None:intergenic
AACGCCAACACCGCCTTCAC+CGG	0.468847	8_4:-46226232	None:intergenic
AACGTTGAAGGAAGGAAGGA+AGG	0.609399	8_4:+46224153	Msa1328400:five_prime_UTR
AACTACTGTTATTAGGGTTA+AGG	0.348943	8_4:+46224559	Msa1328400:five_prime_UTR
AACTCATGTTAAAAGCACTT+GGG	0.578823	8_4:+46225069	Msa1328400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACACAAAAACAAACGTTGA+AGG	+	chr8_4:46224141-46224160	Msa1328400:five_prime_UTR	30.0%
AAGTATATGTCTCCTTGTTA+TGG	-	chr8_4:46224337-46224356	None:intergenic	30.0%
CAGTTGAGAGAGAATTAATT+GGG	-	chr8_4:46224448-46224467	None:intergenic	30.0%
ACAGTTGAGAGAGAATTAAT+TGG	-	chr8_4:46224449-46224468	None:intergenic	30.0%
AACTACTGTTATTAGGGTTA+AGG	+	chr8_4:46224559-46224578	Msa1328400:five_prime_UTR	30.0%
GTTCATATAATAGTACCTGA+AGG	-	chr8_4:46225211-46225230	None:intergenic	30.0%
CTTAACCATAACACTGAAAA+GGG	-	chr8_4:46225343-46225362	None:intergenic	30.0%
TCTTAACCATAACACTGAAA+AGG	-	chr8_4:46225344-46225363	None:intergenic	30.0%
AGGAAAAACAGTTTCTTTCT+TGG	-	chr8_4:46225642-46225661	None:intergenic	30.0%
TGCATTCTGTTGTATTGTTA+AGG	+	chr8_4:46225911-46225930	Msa1328400:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	46224039	46226750	46224039	ID=Msa1328400;Name=Msa1328400
chr8_4	mRNA	46224039	46226750	46224039	ID=Msa1328400-mRNA-1;Parent=Msa1328400;Name=Msa1328400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=563\|1\|1\|1\|1\|1\|2\|346\|338
chr8_4	exon	46224039	46225214	46224039	ID=Msa1328400-mRNA-1:exon:13077;Parent=Msa1328400-mRNA-1
chr8_4	exon	46226001	46226750	46226001	ID=Msa1328400-mRNA-1:exon:13078;Parent=Msa1328400-mRNA-1
chr8_4	five_prime_UTR	46224039	46224601	46224039	ID=Msa1328400-mRNA-1:five_prime_utr;Parent=Msa1328400-mRNA-1
chr8_4	CDS	46224602	46225214	46224602	ID=Msa1328400-mRNA-1:cds;Parent=Msa1328400-mRNA-1
chr8_4	CDS	46226001	46226404	46226001	ID=Msa1328400-mRNA-1:cds;Parent=Msa1328400-mRNA-1
chr8_4	three_prime_UTR	46226405	46226750	46226405	ID=Msa1328400-mRNA-1:three_prime_utr;Parent=Msa1328400-mRNA-1

Gene Sequence

>Msa1328400

ATGGCTGGTTTGTTCTCATTAGGAGGTGGTGGAAGAGGAAACCAAGGAGAAGAATCACAACAACAAGGTCATATTCCACCACAAGAGACACTATTTTGGTACAACAAAAACGATGATGTTTCATCTTACAACAGAGGTAATTTAGAATTATGGAACCAACACCAACAACAACAGCACCAACAACACCACCAGCAACAACAACAACAAGATGATATGCATGCTGCACGGCCGTTCTTCCCGCGAGATCTCTACGGTCCAGGAGTTGGTTTAGGTGTTGGACCAAGCAGGGTTTCATCAGATGATCATGAACATCAATCGTCGTCGAGATCGGCAGCGTTTACTATGGCGATGAGGTCTTCTTCTTCAGCTGAAGGGATAAGTTGTCAAGATTGTGGTAACCAAGCTAAGAAAGATTGTCCTCACATGAGATGTAGAACTTGTTGTAAGAGTCGTGGCTTTCAGTGTCAAACTCATGTTAAAAGCACTTGGGTTCCTGCTTCTAGACGCCGTGAGCGTCAACAACAGTTATCTTCTTCTCCTTTACAAAGAGATATTTCAAAACGCCCTAGAGATGGTTCAAATGCTCTTGTTTCAACTCGTAATTTTCCTTCAGGGTTAGAGGAAGCAAATTTTCCTGCTGTGGTAAGCTCACCAGCGGAGTTCAGGTGTGTAAGGGTTAGTTCAATTGATGATGCAGATGATAGGTATGCATATCAAACGGCTGTTAACATTGGAGGACATTTGTTCAAAGGAATTCTCTATGATTTTGGTCCAGAAAGTAGTACCAACAATAACAGCAACAATAGTAATTATAATAATAGTAATTACATGATTGGGGAGACTTCCGGTGAAGGCGGTGTTGGCGTTGCTCAACCGTTGAACCTAATCACCGACTCTGATACCACTGTTGTTGCCTCCTCGGGCGCACTTGTTGATCCTTCTTCATTGTATTCGGCTCCGATTAATGCCTTCATGACGGCTAGTGGTACGCAATTTTTCCCTCGCCCAAGATCTTGA

Protein sequence

>Msa1328400

MAGLFSLGGGGRGNQGEESQQQGHIPPQETLFWYNKNDDVSSYNRGNLELWNQHQQQQHQQHHQQQQQQDDMHAARPFFPRDLYGPGVGLGVGPSRVSSDDHEHQSSSRSAAFTMAMRSSSSAEGISCQDCGNQAKKDCPHMRCRTCCKSRGFQCQTHVKSTWVPASRRRERQQQLSSSPLQRDISKRPRDGSNALVSTRNFPSGLEEANFPAVVSSPAEFRCVRVSSIDDADDRYAYQTAVNIGGHLFKGILYDFGPESSTNNNSNNSNYNNSNYMIGETSGEGGVGVAQPLNLITDSDTTVVASSGALVDPSSLYSAPINAFMTASGTQFFPRPRS*