AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1328400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1328400 MtrunA17_Chr8g0372461 95.858 338 7 2 1 338 1 331 0.0 663
Msa1328400 MtrunA17_Chr5g0404781 52.342 363 108 13 1 338 1 323 2.90e-91 275
Msa1328400 MtrunA17_Chr3g0142171 43.064 346 126 16 1 333 1 288 2.47e-63 203
Msa1328400 MtrunA17_Chr1g0155791 51.915 235 75 7 126 338 120 338 4.86e-58 191
Msa1328400 MtrunA17_Chr5g0441921 50.323 155 54 4 128 259 124 278 8.16e-41 145
Msa1328400 MtrunA17_Chr4g0035591 47.887 142 65 2 124 265 16 148 2.84e-40 140
Msa1328400 MtrunA17_Chr3g0082511 39.869 153 69 1 128 257 134 286 1.49e-33 126
Msa1328400 MtrunA17_Chr8g0353111 39.884 173 73 3 115 257 111 282 4.25e-33 125
Msa1328400 MtrunA17_Chr0c01g0489181 56.410 39 17 0 124 162 63 101 6.08e-14 67.4
Msa1328400 MtrunA17_Chr2g0281741 61.538 39 15 0 124 162 146 184 4.30e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1328400 AT3G51060.1 44.275 393 141 11 1 338 1 370 1.95e-80 249
Msa1328400 AT5G66350.1 46.113 373 124 15 1 338 1 331 4.49e-74 232
Msa1328400 AT5G66350.3 45.856 362 121 14 1 327 1 322 7.69e-72 226
Msa1328400 AT5G66350.2 45.983 361 120 14 1 326 1 321 1.19e-71 226
Msa1328400 AT1G19790.2 42.781 374 147 16 1 337 1 344 3.34e-69 220
Msa1328400 AT1G19790.1 42.781 374 147 16 1 337 1 344 3.34e-69 220
Msa1328400 AT1G75520.1 41.755 376 151 15 1 338 1 346 1.07e-62 203
Msa1328400 AT4G36260.1 39.889 361 150 13 1 336 1 319 2.15e-54 181
Msa1328400 AT2G21400.2 47.701 174 83 3 125 294 7 176 6.29e-49 162
Msa1328400 AT2G21400.4 47.701 174 83 3 125 294 6 175 7.56e-49 162
Msa1328400 AT2G21400.3 47.647 170 74 3 125 294 7 161 3.44e-48 160
Msa1328400 AT2G21400.1 47.647 170 74 3 125 294 6 160 3.68e-48 160
Msa1328400 AT2G18120.1 53.548 155 57 3 117 262 61 209 2.68e-47 159
Msa1328400 AT2G18120.2 52.288 153 60 2 117 262 61 207 1.41e-46 157
Msa1328400 AT5G12330.1 47.205 161 55 4 128 259 112 271 2.13e-38 139
Msa1328400 AT5G12330.2 47.205 161 55 4 128 259 112 271 2.13e-38 139
Msa1328400 AT5G12330.4 47.205 161 55 4 128 259 199 358 3.94e-38 140
Msa1328400 AT5G33210.1 42.405 158 70 5 94 236 14 165 4.31e-29 110
Msa1328400 AT5G33210.2 61.176 85 23 2 126 200 15 99 3.90e-27 103
Msa1328400 AT3G54430.1 42.553 141 59 6 128 257 41 170 9.30e-26 102
Msa1328400 AT5G12330.3 82.609 46 8 0 128 173 112 157 3.58e-21 90.9

Find 114 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTGTTGGTACTACTTTC+TGG 0.121760 8_4:-46226159 None:intergenic
TTAAAGAAACTACTGTTATT+AGG 0.143287 8_4:+46224552 Msa1328400:five_prime_UTR
AATGATTGAAACACAAATTT+TGG 0.146618 8_4:-46226483 None:intergenic
AAAGGAATTCTCTATGATTT+TGG 0.200894 8_4:+46226135 Msa1328400:CDS
AGAAGAAAGAGGAGTTATTA+TGG 0.207274 8_4:+46226416 Msa1328400:three_prime_UTR
ACAGTTGAGAGAGAATTAAT+TGG 0.214572 8_4:-46224446 None:intergenic
TTTCTATGTGTACTCTATAA+TGG 0.229112 8_4:+46226693 Msa1328400:three_prime_UTR
ACAGAGGTAATTTAGAATTA+TGG 0.238498 8_4:+46224732 Msa1328400:CDS
CACCACAAGAGACACTATTT+TGG 0.271662 8_4:+46224678 Msa1328400:CDS
TAAAGAAACTACTGTTATTA+GGG 0.274863 8_4:+46224553 Msa1328400:five_prime_UTR
TGATCCTTCTTCATTGTATT+CGG 0.283339 8_4:+46226320 Msa1328400:CDS
AATAATAGTAATTACATGAT+TGG 0.330195 8_4:+46226201 Msa1328400:CDS
GAAGAAAGAGGAGTTATTAT+GGG 0.330694 8_4:+46226417 Msa1328400:three_prime_UTR
ATAATAGTAATTACATGATT+GGG 0.347728 8_4:+46226202 Msa1328400:CDS
AACTACTGTTATTAGGGTTA+AGG 0.348943 8_4:+46224559 Msa1328400:five_prime_UTR
AAGTATATGTCTCCTTGTTA+TGG 0.356150 8_4:-46224334 None:intergenic
TACCACTGTTGTTGCCTCCT+CGG 0.375579 8_4:+46226287 Msa1328400:CDS
AAGCTATAGCTGCTGCTAGC+TGG 0.376069 8_4:+46224180 Msa1328400:five_prime_UTR
ATGGCTGGTTTGTTCTCATT+AGG 0.380675 8_4:+46224602 Msa1328400:CDS
TAAGCTCACCAGCGGAGTTC+AGG 0.381652 8_4:+46226031 Msa1328400:CDS
TACTCTATAATGGACTTCTA+TGG 0.385956 8_4:+46226703 Msa1328400:three_prime_UTR
CAGCGGAGTTCAGGTGTGTA+AGG 0.390315 8_4:+46226040 Msa1328400:CDS
GTTGTTGTGATTCTTCTCCT+TGG 0.392328 8_4:-46224643 None:intergenic
TACATGATTGGGGAGACTTC+CGG 0.393571 8_4:+46226213 Msa1328400:CDS
GTTGTTGCTGGTGGTGTTGT+TGG 0.401531 8_4:-46224778 None:intergenic
GTTGTTGGTGCTGTTGTTGT+TGG 0.405595 8_4:-46224763 None:intergenic
CAGTTGAGAGAGAATTAATT+GGG 0.406433 8_4:-46224445 None:intergenic
TACGGTCCAGGAGTTGGTTT+AGG 0.417465 8_4:+46224851 Msa1328400:CDS
ACCACTGTTGTTGCCTCCTC+GGG 0.418082 8_4:+46226288 Msa1328400:CDS
ACTATTGTTGCTGTTATTGT+TGG 0.418687 8_4:-46226172 None:intergenic
AGCTATAGCTGCTGCTAGCT+GGG 0.421738 8_4:+46224181 Msa1328400:five_prime_UTR
CCAGGAGTTGGTTTAGGTGT+TGG 0.426741 8_4:+46224857 Msa1328400:CDS
GTTTAGGTGTTGGACCAAGC+AGG 0.429835 8_4:+46224867 Msa1328400:CDS
GGTGCTGTTGTTGTTGGTGT+TGG 0.440333 8_4:-46224757 None:intergenic
TGGTATCAGAGTCGGTGATT+AGG 0.442544 8_4:-46226269 None:intergenic
TATGTCTCCTTGTTATGGTT+GGG 0.444674 8_4:-46224329 None:intergenic
GCCGTCATGAAGGCATTAAT+CGG 0.448399 8_4:-46226345 None:intergenic
ATAGAAAGAAACAATAGCTT+TGG 0.451472 8_4:-46226677 None:intergenic
GAGGACAATCTTTCTTAGCT+TGG 0.464592 8_4:-46225000 None:intergenic
TTCTTCTTCAAAGAAATGGC+TGG 0.464677 8_4:+46224587 Msa1328400:exon
TTGTTCTCATTAGGAGGTGG+TGG 0.466253 8_4:+46224611 Msa1328400:CDS
AACGCCAACACCGCCTTCAC+CGG 0.468847 8_4:-46226232 None:intergenic
TTTGAAATATCTCTTTGTAA+AGG 0.469835 8_4:-46225139 None:intergenic
TTGAAGGAAGGAAGGAAGGA+AGG 0.475608 8_4:+46224157 Msa1328400:five_prime_UTR
ACTTCCGGTGAAGGCGGTGT+TGG 0.482952 8_4:+46226228 Msa1328400:CDS
CAATTGATGATGCAGATGAT+AGG 0.486383 8_4:+46226070 Msa1328400:CDS
GAGATCGGCAGCGTTTACTA+TGG 0.486558 8_4:+46224925 Msa1328400:CDS
TCATTAGGAGGTGGTGGAAG+AGG 0.489984 8_4:+46224617 Msa1328400:CDS
ATATGTCTCCTTGTTATGGT+TGG 0.492221 8_4:-46224330 None:intergenic
AAACTCATGTTAAAAGCACT+TGG 0.502171 8_4:+46225068 Msa1328400:CDS
ACATCAATCGTCGTCGAGAT+CGG 0.503948 8_4:+46224910 Msa1328400:CDS
AAACAAAATTGAGAAGAAAG+AGG 0.504247 8_4:+46226405 Msa1328400:three_prime_UTR
GGTTTGTTCTCATTAGGAGG+TGG 0.504419 8_4:+46224608 Msa1328400:CDS
GCTGGTGAGCTTACCACAGC+AGG 0.505962 8_4:-46226021 None:intergenic
CGCTCACGGCGTCTAGAAGC+AGG 0.506968 8_4:-46225094 None:intergenic
AGGAGTTATTATGGGGATCA+TGG 0.508047 8_4:+46226425 Msa1328400:three_prime_UTR
AAAATGAGATTATTATATGA+AGG 0.508828 8_4:+46226575 Msa1328400:three_prime_UTR
AGGTCTTCTTCTTCAGCTGA+AGG 0.512980 8_4:+46224953 Msa1328400:CDS
GATCTCTACGGTCCAGGAGT+TGG 0.516227 8_4:+46224845 Msa1328400:CDS
GCTGGTTTGTTCTCATTAGG+AGG 0.517479 8_4:+46224605 Msa1328400:CDS
ATTGGAGGACATTTGTTCAA+AGG 0.517913 8_4:+46226117 Msa1328400:CDS
AGAGCATTTGAACCATCTCT+AGG 0.524317 8_4:-46225166 None:intergenic
ATTGGGGAGACTTCCGGTGA+AGG 0.525108 8_4:+46226219 Msa1328400:CDS
TTTAGGTGTTGGACCAAGCA+GGG 0.533902 8_4:+46224868 Msa1328400:CDS
TCAAATTTACCTCTACTACT+TGG 0.536171 8_4:-46226529 None:intergenic
GTAAAAGAGAAGTATATATG+TGG 0.544134 8_4:-46226461 None:intergenic
GGGATAAGTTGTCAAGATTG+TGG 0.548229 8_4:+46224974 Msa1328400:CDS
ATAACTGTTGTTGACGCTCA+CGG 0.552876 8_4:-46225108 None:intergenic
CCAACACCTAAACCAACTCC+TGG 0.554565 8_4:-46224857 None:intergenic
CAAAATAGTGTCTCTTGTGG+TGG 0.555466 8_4:-46224677 None:intergenic
TAGAGATCTCGCGGGAAGAA+CGG 0.556385 8_4:-46224830 None:intergenic
TATCAAACGGCTGTTAACAT+TGG 0.556930 8_4:+46226099 Msa1328400:CDS
TGACCATGACATTGTTGTTG+TGG 0.557428 8_4:+46226506 Msa1328400:three_prime_UTR
TGATAGGTATGCATATCAAA+CGG 0.559733 8_4:+46226086 Msa1328400:CDS
AACAAACGTTGAAGGAAGGA+AGG 0.563270 8_4:+46224149 Msa1328400:five_prime_UTR
CAAACGGCTGTTAACATTGG+AGG 0.566332 8_4:+46226102 Msa1328400:CDS
CGTACCACTAGCCGTCATGA+AGG 0.569674 8_4:-46226355 None:intergenic
ATTTCAAAACGCCCTAGAGA+TGG 0.570524 8_4:+46225154 Msa1328400:CDS
TTCTTCCCGCGAGATCTCTA+CGG 0.572634 8_4:+46224833 Msa1328400:CDS
TCATCTGATGAAACCCTGCT+TGG 0.577114 8_4:-46224881 None:intergenic
AACTCATGTTAAAAGCACTT+GGG 0.578823 8_4:+46225069 Msa1328400:CDS
TAAAAGAGAAGTATATATGT+GGG 0.583599 8_4:-46226460 None:intergenic
CTTACACACCTGAACTCCGC+TGG 0.583863 8_4:-46226039 None:intergenic
TAATAGTAATTACATGATTG+GGG 0.584644 8_4:+46226203 Msa1328400:CDS
GGTGGTGGAAGAGGAAACCA+AGG 0.593423 8_4:+46224626 Msa1328400:CDS
GGTCTTCTTCTTCAGCTGAA+GGG 0.597588 8_4:+46224954 Msa1328400:CDS
AAGAAAGAGGAGTTATTATG+GGG 0.598652 8_4:+46226418 Msa1328400:three_prime_UTR
GAGCATTTGAACCATCTCTA+GGG 0.600057 8_4:-46225165 None:intergenic
AGCGGAGTTCAGGTGTGTAA+GGG 0.600996 8_4:+46226041 Msa1328400:CDS
TTGTTGTGGCCAAGTAGTAG+AGG 0.601864 8_4:+46226520 Msa1328400:three_prime_UTR
AGGATCAACAAGTGCGCCCG+AGG 0.604938 8_4:-46226304 None:intergenic
GGAGCCGAATACAATGAAGA+AGG 0.606433 8_4:-46226324 None:intergenic
AACGTTGAAGGAAGGAAGGA+AGG 0.609399 8_4:+46224153 Msa1328400:five_prime_UTR
GAGTCGGTGATTAGGTTCAA+CGG 0.611827 8_4:-46226261 None:intergenic
AATGCCTTCATGACGGCTAG+TGG 0.616126 8_4:+46226351 Msa1328400:CDS
ATCAACAAGTGCGCCCGAGG+AGG 0.617141 8_4:-46226301 None:intergenic
GGGGAGACTTCCGGTGAAGG+CGG 0.620239 8_4:+46226222 Msa1328400:CDS
AACAACAGTGGTATCAGAGT+CGG 0.622301 8_4:-46226277 None:intergenic
AGAACTTGTTGTAAGAGTCG+TGG 0.626794 8_4:+46225034 Msa1328400:CDS
TCCGATTAATGCCTTCATGA+CGG 0.631102 8_4:+46226344 Msa1328400:CDS
GGACAGAAAACAGAAACAGA+CGG 0.636265 8_4:-46224256 None:intergenic
TGCTGTGGTAAGCTCACCAG+CGG 0.638941 8_4:+46226023 Msa1328400:CDS
GATGTTTCATCTTACAACAG+AGG 0.641152 8_4:+46224716 Msa1328400:CDS
CCTGGACCGTAGAGATCTCG+CGG 0.642572 8_4:-46224839 None:intergenic
TGGCCACAACAACAATGTCA+TGG 0.643734 8_4:-46226509 None:intergenic
TACCAAAATAGTGTCTCTTG+TGG 0.654055 8_4:-46224680 None:intergenic
AAGATGATATGCATGCTGCA+CGG 0.670001 8_4:+46224807 Msa1328400:CDS
GCCCGAGGAGGCAACAACAG+TGG 0.675386 8_4:-46226289 None:intergenic
GACGCACACAAAAGAAAACA+CGG 0.689183 8_4:+46224396 Msa1328400:five_prime_UTR
CAGCGTTTACTATGGCGATG+AGG 0.700377 8_4:+46224933 Msa1328400:CDS
CAAGTTCTACATCTCATGTG+AGG 0.701177 8_4:-46225019 None:intergenic
CAGACGGCTAAACAGTGTCG+TGG 0.701923 8_4:-46224240 None:intergenic
CTGGACCGTAGAGATCTCGC+GGG 0.715374 8_4:-46224838 None:intergenic
GGAGAAGAATCACAACAACA+AGG 0.732085 8_4:+46224647 Msa1328400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAATAATAATAATAA+AGG - chr8_4:46224056-46224075 None:intergenic 0.0%
!! TTAATAATAATAATAAAGGA+AGG - chr8_4:46224052-46224071 None:intergenic 10.0%
!!! AAGAATAAATATAGTATTTT+TGG - chr8_4:46226637-46226656 None:intergenic 10.0%
!! AAATAATAAAAAAAAGCTGA+TGG - chr8_4:46225548-46225567 None:intergenic 15.0%
!! AATAATAGTAATTACATGAT+TGG + chr8_4:46226201-46226220 Msa1328400:CDS 15.0%
!! ATAATAGTAATTACATGATT+GGG + chr8_4:46226202-46226221 Msa1328400:CDS 15.0%
!! AAAATGAGATTATTATATGA+AGG + chr8_4:46226575-46226594 Msa1328400:three_prime_UTR 15.0%
!! TATACAGAAAAACATAAGTT+AGG + chr8_4:46224113-46224132 Msa1328400:five_prime_UTR 20.0%
!! AATTGAAGTTAAGAAAAAGA+AGG - chr8_4:46224280-46224299 None:intergenic 20.0%
!!! ATTTGTGAAAAATGGTTTTT+GGG - chr8_4:46224425-46224444 None:intergenic 20.0%
!!! CTAAATAGGTTTTTTGTTTT+TGG - chr8_4:46224502-46224521 None:intergenic 20.0%
!! CAAAAACAAAAAACCTATTT+AGG + chr8_4:46224500-46224519 Msa1328400:five_prime_UTR 20.0%
!! TTAAAGAAACTACTGTTATT+AGG + chr8_4:46224552-46224571 Msa1328400:five_prime_UTR 20.0%
!! TAAAGAAACTACTGTTATTA+GGG + chr8_4:46224553-46224572 Msa1328400:five_prime_UTR 20.0%
!!! TTTTTTCTTCTTCAAAGAAA+TGG + chr8_4:46224583-46224602 Msa1328400:exon 20.0%
!!! TTTGAAATATCTCTTTGTAA+AGG - chr8_4:46225142-46225161 None:intergenic 20.0%
!!! TTGCTTTTGTTTTTTAGTTA+AGG + chr8_4:46225247-46225266 Msa1328400:intron 20.0%
!!! TGTTTTTCACAAAAGAAAAA+GGG - chr8_4:46225596-46225615 None:intergenic 20.0%
!!! ATGTTTTTCACAAAAGAAAA+AGG - chr8_4:46225597-46225616 None:intergenic 20.0%
!! ATTAAAAAATAAAGGGTTAG+AGG + chr8_4:46225987-46226006 Msa1328400:intron 20.0%
!! TAATAGTAATTACATGATTG+GGG + chr8_4:46226203-46226222 Msa1328400:CDS 20.0%
!!! AATTTTGTTTTCAAGATCTT+GGG - chr8_4:46226395-46226414 None:intergenic 20.0%
!!! TAAAAGAGAAGTATATATGT+GGG - chr8_4:46226463-46226482 None:intergenic 20.0%
!!! AATGATTGAAACACAAATTT+TGG - chr8_4:46226486-46226505 None:intergenic 20.0%
!! GATTTGTGAAAAATGGTTTT+TGG - chr8_4:46224426-46224445 None:intergenic 25.0%
! TAATTGGGATTTGTGAAAAA+TGG - chr8_4:46224433-46224452 None:intergenic 25.0%
!!! ACCTATTTAGGTTTTTGATA+TGG + chr8_4:46224512-46224531 Msa1328400:five_prime_UTR 25.0%
! TCCATATCAAAAACCTAAAT+AGG - chr8_4:46224516-46224535 None:intergenic 25.0%
!! ACAGAGGTAATTTAGAATTA+TGG + chr8_4:46224732-46224751 Msa1328400:CDS 25.0%
!!! TTTGTTTTTTAGTTAAGGAC+TGG + chr8_4:46225252-46225271 Msa1328400:intron 25.0%
!! AAAACAAAGAGAGAATCTTT+TGG + chr8_4:46225391-46225410 Msa1328400:intron 25.0%
! AAAACTTTGTCATCAGTATT+TGG - chr8_4:46225444-46225463 None:intergenic 25.0%
!! AAATACTGATGACAAAGTTT+TGG + chr8_4:46225443-46225462 Msa1328400:intron 25.0%
!! AAAGGAATTCTCTATGATTT+TGG + chr8_4:46226135-46226154 Msa1328400:CDS 25.0%
!!! CAATTTTGTTTTCAAGATCT+TGG - chr8_4:46226396-46226415 None:intergenic 25.0%
! AAACAAAATTGAGAAGAAAG+AGG + chr8_4:46226405-46226424 Msa1328400:three_prime_UTR 25.0%
!! GTAAAAGAGAAGTATATATG+TGG - chr8_4:46226464-46226483 None:intergenic 25.0%
! ATAGAAAGAAACAATAGCTT+TGG - chr8_4:46226680-46226699 None:intergenic 25.0%
! TTTCTATGTGTACTCTATAA+TGG + chr8_4:46226693-46226712 Msa1328400:three_prime_UTR 25.0%
TACACAAAAACAAACGTTGA+AGG + chr8_4:46224141-46224160 Msa1328400:five_prime_UTR 30.0%
AAGTATATGTCTCCTTGTTA+TGG - chr8_4:46224337-46224356 None:intergenic 30.0%
! CTGTGTTTTGTGTAATTTTC+TGG + chr8_4:46224372-46224391 Msa1328400:five_prime_UTR 30.0%
CAGTTGAGAGAGAATTAATT+GGG - chr8_4:46224448-46224467 None:intergenic 30.0%
ACAGTTGAGAGAGAATTAAT+TGG - chr8_4:46224449-46224468 None:intergenic 30.0%
AACTACTGTTATTAGGGTTA+AGG + chr8_4:46224559-46224578 Msa1328400:five_prime_UTR 30.0%
!! AAACTCATGTTAAAAGCACT+TGG + chr8_4:46225068-46225087 Msa1328400:CDS 30.0%
!! AACTCATGTTAAAAGCACTT+GGG + chr8_4:46225069-46225088 Msa1328400:CDS 30.0%
GTTCATATAATAGTACCTGA+AGG - chr8_4:46225211-46225230 None:intergenic 30.0%
CTTAACCATAACACTGAAAA+GGG - chr8_4:46225343-46225362 None:intergenic 30.0%
TCTTAACCATAACACTGAAA+AGG - chr8_4:46225344-46225363 None:intergenic 30.0%
! AAGCTGATGGATATGAAATA+TGG - chr8_4:46225535-46225554 None:intergenic 30.0%
AGGAAAAACAGTTTCTTTCT+TGG - chr8_4:46225642-46225661 None:intergenic 30.0%
TGCATTCTGTTGTATTGTTA+AGG + chr8_4:46225911-46225930 Msa1328400:intron 30.0%
TGATAGGTATGCATATCAAA+CGG + chr8_4:46226086-46226105 Msa1328400:CDS 30.0%
TTATTGTTGGTACTACTTTC+TGG - chr8_4:46226162-46226181 None:intergenic 30.0%
! ACTATTGTTGCTGTTATTGT+TGG - chr8_4:46226175-46226194 None:intergenic 30.0%
TGATCCTTCTTCATTGTATT+CGG + chr8_4:46226320-46226339 Msa1328400:CDS 30.0%
AGAAGAAAGAGGAGTTATTA+TGG + chr8_4:46226416-46226435 Msa1328400:three_prime_UTR 30.0%
GAAGAAAGAGGAGTTATTAT+GGG + chr8_4:46226417-46226436 Msa1328400:three_prime_UTR 30.0%
AAGAAAGAGGAGTTATTATG+GGG + chr8_4:46226418-46226437 Msa1328400:three_prime_UTR 30.0%
TCAAATTTACCTCTACTACT+TGG - chr8_4:46226532-46226551 None:intergenic 30.0%
!! TACTCTATAATGGACTTCTA+TGG + chr8_4:46226703-46226722 Msa1328400:three_prime_UTR 30.0%
CAAAAACAAACGTTGAAGGA+AGG + chr8_4:46224145-46224164 Msa1328400:five_prime_UTR 35.0%
! TATGTCTCCTTGTTATGGTT+GGG - chr8_4:46224332-46224351 None:intergenic 35.0%
! ATATGTCTCCTTGTTATGGT+TGG - chr8_4:46224333-46224352 None:intergenic 35.0%
TTCTTCTTCAAAGAAATGGC+TGG + chr8_4:46224587-46224606 Msa1328400:exon 35.0%
TACCAAAATAGTGTCTCTTG+TGG - chr8_4:46224683-46224702 None:intergenic 35.0%
GATGTTTCATCTTACAACAG+AGG + chr8_4:46224716-46224735 Msa1328400:CDS 35.0%
! TCAACTCGTAATTTTCCTTC+AGG + chr8_4:46225193-46225212 Msa1328400:CDS 35.0%
TACTGTGACATAAGGTGTAA+GGG + chr8_4:46225280-46225299 Msa1328400:intron 35.0%
! ATTGACCCTTTTCAGTGTTA+TGG + chr8_4:46225335-46225354 Msa1328400:intron 35.0%
!!! GAGAGAATCTTTTGGTTTAG+AGG + chr8_4:46225399-46225418 Msa1328400:intron 35.0%
ATAAACACACGCAATCGATA+CGG - chr8_4:46225492-46225511 None:intergenic 35.0%
! CTGATGGATATGAAATATGG+TGG - chr8_4:46225532-46225551 None:intergenic 35.0%
AACAGTTTCTTTCTTGGTCA+GGG - chr8_4:46225636-46225655 None:intergenic 35.0%
AAACAGTTTCTTTCTTGGTC+AGG - chr8_4:46225637-46225656 None:intergenic 35.0%
!! CAATTGATGATGCAGATGAT+AGG + chr8_4:46226070-46226089 Msa1328400:CDS 35.0%
TATCAAACGGCTGTTAACAT+TGG + chr8_4:46226099-46226118 Msa1328400:CDS 35.0%
ATTGGAGGACATTTGTTCAA+AGG + chr8_4:46226117-46226136 Msa1328400:CDS 35.0%
AACAAACGTTGAAGGAAGGA+AGG + chr8_4:46224149-46224168 Msa1328400:five_prime_UTR 40.0%
GGACAGAAAACAGAAACAGA+CGG - chr8_4:46224259-46224278 None:intergenic 40.0%
! CTCTTTTCCCAACCATAACA+AGG + chr8_4:46224322-46224341 Msa1328400:five_prime_UTR 40.0%
GACGCACACAAAAGAAAACA+CGG + chr8_4:46224396-46224415 Msa1328400:five_prime_UTR 40.0%
!! ATGGCTGGTTTGTTCTCATT+AGG + chr8_4:46224602-46224621 Msa1328400:CDS 40.0%
GTTGTTGTGATTCTTCTCCT+TGG - chr8_4:46224646-46224665 None:intergenic 40.0%
GGAGAAGAATCACAACAACA+AGG + chr8_4:46224647-46224666 Msa1328400:CDS 40.0%
CAAAATAGTGTCTCTTGTGG+TGG - chr8_4:46224680-46224699 None:intergenic 40.0%
! CACCACAAGAGACACTATTT+TGG + chr8_4:46224678-46224697 Msa1328400:CDS 40.0%
!! CATCTTGTTGTTGTTGTTGC+TGG - chr8_4:46224793-46224812 None:intergenic 40.0%
AAGATGATATGCATGCTGCA+CGG + chr8_4:46224807-46224826 Msa1328400:CDS 40.0%
GGGATAAGTTGTCAAGATTG+TGG + chr8_4:46224974-46224993 Msa1328400:CDS 40.0%
GAGGACAATCTTTCTTAGCT+TGG - chr8_4:46225003-46225022 None:intergenic 40.0%
CAAGTTCTACATCTCATGTG+AGG - chr8_4:46225022-46225041 None:intergenic 40.0%
!! AGAACTTGTTGTAAGAGTCG+TGG + chr8_4:46225034-46225053 Msa1328400:CDS 40.0%
ATAACTGTTGTTGACGCTCA+CGG - chr8_4:46225111-46225130 None:intergenic 40.0%
ATTTCAAAACGCCCTAGAGA+TGG + chr8_4:46225154-46225173 Msa1328400:CDS 40.0%
GAGCATTTGAACCATCTCTA+GGG - chr8_4:46225168-46225187 None:intergenic 40.0%
! AGAGCATTTGAACCATCTCT+AGG - chr8_4:46225169-46225188 None:intergenic 40.0%
TGGACTTGTACTGTGACATA+AGG + chr8_4:46225272-46225291 Msa1328400:intron 40.0%
GTACTGTGACATAAGGTGTA+AGG + chr8_4:46225279-46225298 Msa1328400:intron 40.0%
ACACTGAAAAGGGTCAATCA+CGG - chr8_4:46225333-46225352 None:intergenic 40.0%
GTTATGGTTAAGAGAGTGAG+TGG + chr8_4:46225351-46225370 Msa1328400:intron 40.0%
GAGAGTGAGTGGTGAAATAA+AGG + chr8_4:46225362-46225381 Msa1328400:intron 40.0%
GATTGAGGTGAGAATATGTG+TGG - chr8_4:46225700-46225719 None:intergenic 40.0%
AACAACAGTGGTATCAGAGT+CGG - chr8_4:46226280-46226299 None:intergenic 40.0%
TCCGATTAATGCCTTCATGA+CGG + chr8_4:46226344-46226363 Msa1328400:CDS 40.0%
AGGAGTTATTATGGGGATCA+TGG + chr8_4:46226425-46226444 Msa1328400:three_prime_UTR 40.0%
! TGACCATGACATTGTTGTTG+TGG + chr8_4:46226506-46226525 Msa1328400:three_prime_UTR 40.0%
AACGTTGAAGGAAGGAAGGA+AGG + chr8_4:46224153-46224172 Msa1328400:five_prime_UTR 45.0%
TTGAAGGAAGGAAGGAAGGA+AGG + chr8_4:46224157-46224176 Msa1328400:five_prime_UTR 45.0%
!! GCTGGTTTGTTCTCATTAGG+AGG + chr8_4:46224605-46224624 Msa1328400:CDS 45.0%
! GGTTTGTTCTCATTAGGAGG+TGG + chr8_4:46224608-46224627 Msa1328400:CDS 45.0%
TTGTTCTCATTAGGAGGTGG+TGG + chr8_4:46224611-46224630 Msa1328400:CDS 45.0%
!! GTTGTTGGTGCTGTTGTTGT+TGG - chr8_4:46224766-46224785 None:intergenic 45.0%
!! CTTGTTGTTGTTGTTGCTGG+TGG - chr8_4:46224790-46224809 None:intergenic 45.0%
!! TTTAGGTGTTGGACCAAGCA+GGG + chr8_4:46224868-46224887 Msa1328400:CDS 45.0%
TCATCTGATGAAACCCTGCT+TGG - chr8_4:46224884-46224903 None:intergenic 45.0%
ACATCAATCGTCGTCGAGAT+CGG + chr8_4:46224910-46224929 Msa1328400:CDS 45.0%
AGGTCTTCTTCTTCAGCTGA+AGG + chr8_4:46224953-46224972 Msa1328400:CDS 45.0%
GGTCTTCTTCTTCAGCTGAA+GGG + chr8_4:46224954-46224973 Msa1328400:CDS 45.0%
GGAATGTGCTAAGAAAGAGG+AGG - chr8_4:46225662-46225681 None:intergenic 45.0%
GAGGGAATGTGCTAAGAAAG+AGG - chr8_4:46225665-46225684 None:intergenic 45.0%
GTGTGGATGTGAAAGAGAGA+GGG - chr8_4:46225683-46225702 None:intergenic 45.0%
TGTGTGGATGTGAAAGAGAG+AGG - chr8_4:46225684-46225703 None:intergenic 45.0%
! GGAAGCAAATTTTCCTGCTG+TGG + chr8_4:46226008-46226027 Msa1328400:CDS 45.0%
CAAACGGCTGTTAACATTGG+AGG + chr8_4:46226102-46226121 Msa1328400:CDS 45.0%
TACATGATTGGGGAGACTTC+CGG + chr8_4:46226213-46226232 Msa1328400:CDS 45.0%
GAGTCGGTGATTAGGTTCAA+CGG - chr8_4:46226264-46226283 None:intergenic 45.0%
TGGTATCAGAGTCGGTGATT+AGG - chr8_4:46226272-46226291 None:intergenic 45.0%
GGAGCCGAATACAATGAAGA+AGG - chr8_4:46226327-46226346 None:intergenic 45.0%
!! GCCGTCATGAAGGCATTAAT+CGG - chr8_4:46226348-46226367 None:intergenic 45.0%
!! GTTTTCAAGATCTTGGGCGA+GGG - chr8_4:46226389-46226408 None:intergenic 45.0%
!! TGTTTTCAAGATCTTGGGCG+AGG - chr8_4:46226390-46226409 None:intergenic 45.0%
TGGCCACAACAACAATGTCA+TGG - chr8_4:46226512-46226531 None:intergenic 45.0%
TTGTTGTGGCCAAGTAGTAG+AGG + chr8_4:46226520-46226539 Msa1328400:three_prime_UTR 45.0%
!!! TTTGTTTTATTAAAAAATAA+AGG + chr8_4:46225979-46225998 Msa1328400:intron 5.0%
!!! TTGTTTTATTAAAAAATAAA+GGG + chr8_4:46225980-46225999 Msa1328400:intron 5.0%
AAGCTATAGCTGCTGCTAGC+TGG + chr8_4:46224180-46224199 Msa1328400:five_prime_UTR 50.0%
AGCTATAGCTGCTGCTAGCT+GGG + chr8_4:46224181-46224200 Msa1328400:five_prime_UTR 50.0%
!! TCATTAGGAGGTGGTGGAAG+AGG + chr8_4:46224617-46224636 Msa1328400:CDS 50.0%
!! GGTGCTGTTGTTGTTGGTGT+TGG - chr8_4:46224760-46224779 None:intergenic 50.0%
!! GTTGTTGCTGGTGGTGTTGT+TGG - chr8_4:46224781-46224800 None:intergenic 50.0%
TAGAGATCTCGCGGGAAGAA+CGG - chr8_4:46224833-46224852 None:intergenic 50.0%
TTCTTCCCGCGAGATCTCTA+CGG + chr8_4:46224833-46224852 Msa1328400:CDS 50.0%
! TACGGTCCAGGAGTTGGTTT+AGG + chr8_4:46224851-46224870 Msa1328400:CDS 50.0%
CCAACACCTAAACCAACTCC+TGG - chr8_4:46224860-46224879 None:intergenic 50.0%
! CCAGGAGTTGGTTTAGGTGT+TGG + chr8_4:46224857-46224876 Msa1328400:CDS 50.0%
!! GTTTAGGTGTTGGACCAAGC+AGG + chr8_4:46224867-46224886 Msa1328400:CDS 50.0%
GAGATCGGCAGCGTTTACTA+TGG + chr8_4:46224925-46224944 Msa1328400:CDS 50.0%
CAGCGTTTACTATGGCGATG+AGG + chr8_4:46224933-46224952 Msa1328400:CDS 50.0%
ACACACGCAATCGATACGGT+CGG - chr8_4:46225488-46225507 None:intergenic 50.0%
AGCGGAGTTCAGGTGTGTAA+GGG + chr8_4:46226041-46226060 Msa1328400:CDS 50.0%
TACCACTGTTGTTGCCTCCT+CGG + chr8_4:46226287-46226306 Msa1328400:CDS 50.0%
AATGCCTTCATGACGGCTAG+TGG + chr8_4:46226351-46226370 Msa1328400:CDS 50.0%
CAGACGGCTAAACAGTGTCG+TGG - chr8_4:46224243-46224262 None:intergenic 55.0%
!! GGTGGTGGAAGAGGAAACCA+AGG + chr8_4:46224626-46224645 Msa1328400:CDS 55.0%
GATCTCTACGGTCCAGGAGT+TGG + chr8_4:46224845-46224864 Msa1328400:CDS 55.0%
TGCTGTGGTAAGCTCACCAG+CGG + chr8_4:46226023-46226042 Msa1328400:CDS 55.0%
TAAGCTCACCAGCGGAGTTC+AGG + chr8_4:46226031-46226050 Msa1328400:CDS 55.0%
CTTACACACCTGAACTCCGC+TGG - chr8_4:46226042-46226061 None:intergenic 55.0%
CAGCGGAGTTCAGGTGTGTA+AGG + chr8_4:46226040-46226059 Msa1328400:CDS 55.0%
ATTGGGGAGACTTCCGGTGA+AGG + chr8_4:46226219-46226238 Msa1328400:CDS 55.0%
ACCACTGTTGTTGCCTCCTC+GGG + chr8_4:46226288-46226307 Msa1328400:CDS 55.0%
CGTACCACTAGCCGTCATGA+AGG - chr8_4:46226358-46226377 None:intergenic 55.0%
CTGGACCGTAGAGATCTCGC+GGG - chr8_4:46224841-46224860 None:intergenic 60.0%
CCTGGACCGTAGAGATCTCG+CGG - chr8_4:46224842-46224861 None:intergenic 60.0%
GCTGGTGAGCTTACCACAGC+AGG - chr8_4:46226024-46226043 None:intergenic 60.0%
ACTTCCGGTGAAGGCGGTGT+TGG + chr8_4:46226228-46226247 Msa1328400:CDS 60.0%
AACGCCAACACCGCCTTCAC+CGG - chr8_4:46226235-46226254 None:intergenic 60.0%
ATCAACAAGTGCGCCCGAGG+AGG - chr8_4:46226304-46226323 None:intergenic 60.0%
AGGATCAACAAGTGCGCCCG+AGG - chr8_4:46226307-46226326 None:intergenic 60.0%
CCGCGAGATCTCTACGGTCC+AGG + chr8_4:46224839-46224858 Msa1328400:CDS 65.0%
CGCTCACGGCGTCTAGAAGC+AGG - chr8_4:46225097-46225116 None:intergenic 65.0%
GGGGAGACTTCCGGTGAAGG+CGG + chr8_4:46226222-46226241 Msa1328400:CDS 65.0%
GCCCGAGGAGGCAACAACAG+TGG - chr8_4:46226292-46226311 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 46224039 46226750 46224039 ID=Msa1328400;Name=Msa1328400
chr8_4 mRNA 46224039 46226750 46224039 ID=Msa1328400-mRNA-1;Parent=Msa1328400;Name=Msa1328400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=563|1|1|1|1|1|2|346|338
chr8_4 exon 46224039 46225214 46224039 ID=Msa1328400-mRNA-1:exon:13077;Parent=Msa1328400-mRNA-1
chr8_4 exon 46226001 46226750 46226001 ID=Msa1328400-mRNA-1:exon:13078;Parent=Msa1328400-mRNA-1
chr8_4 five_prime_UTR 46224039 46224601 46224039 ID=Msa1328400-mRNA-1:five_prime_utr;Parent=Msa1328400-mRNA-1
chr8_4 CDS 46224602 46225214 46224602 ID=Msa1328400-mRNA-1:cds;Parent=Msa1328400-mRNA-1
chr8_4 CDS 46226001 46226404 46226001 ID=Msa1328400-mRNA-1:cds;Parent=Msa1328400-mRNA-1
chr8_4 three_prime_UTR 46226405 46226750 46226405 ID=Msa1328400-mRNA-1:three_prime_utr;Parent=Msa1328400-mRNA-1
Gene Sequence

>Msa1328400

ATGGCTGGTTTGTTCTCATTAGGAGGTGGTGGAAGAGGAAACCAAGGAGAAGAATCACAACAACAAGGTCATATTCCACCACAAGAGACACTATTTTGGTACAACAAAAACGATGATGTTTCATCTTACAACAGAGGTAATTTAGAATTATGGAACCAACACCAACAACAACAGCACCAACAACACCACCAGCAACAACAACAACAAGATGATATGCATGCTGCACGGCCGTTCTTCCCGCGAGATCTCTACGGTCCAGGAGTTGGTTTAGGTGTTGGACCAAGCAGGGTTTCATCAGATGATCATGAACATCAATCGTCGTCGAGATCGGCAGCGTTTACTATGGCGATGAGGTCTTCTTCTTCAGCTGAAGGGATAAGTTGTCAAGATTGTGGTAACCAAGCTAAGAAAGATTGTCCTCACATGAGATGTAGAACTTGTTGTAAGAGTCGTGGCTTTCAGTGTCAAACTCATGTTAAAAGCACTTGGGTTCCTGCTTCTAGACGCCGTGAGCGTCAACAACAGTTATCTTCTTCTCCTTTACAAAGAGATATTTCAAAACGCCCTAGAGATGGTTCAAATGCTCTTGTTTCAACTCGTAATTTTCCTTCAGGGTTAGAGGAAGCAAATTTTCCTGCTGTGGTAAGCTCACCAGCGGAGTTCAGGTGTGTAAGGGTTAGTTCAATTGATGATGCAGATGATAGGTATGCATATCAAACGGCTGTTAACATTGGAGGACATTTGTTCAAAGGAATTCTCTATGATTTTGGTCCAGAAAGTAGTACCAACAATAACAGCAACAATAGTAATTATAATAATAGTAATTACATGATTGGGGAGACTTCCGGTGAAGGCGGTGTTGGCGTTGCTCAACCGTTGAACCTAATCACCGACTCTGATACCACTGTTGTTGCCTCCTCGGGCGCACTTGTTGATCCTTCTTCATTGTATTCGGCTCCGATTAATGCCTTCATGACGGCTAGTGGTACGCAATTTTTCCCTCGCCCAAGATCTTGA
Protein sequence

>Msa1328400

MAGLFSLGGGGRGNQGEESQQQGHIPPQETLFWYNKNDDVSSYNRGNLELWNQHQQQQHQQHHQQQQQQDDMHAARPFFPRDLYGPGVGLGVGPSRVSSDDHEHQSSSRSAAFTMAMRSSSSAEGISCQDCGNQAKKDCPHMRCRTCCKSRGFQCQTHVKSTWVPASRRRERQQQLSSSPLQRDISKRPRDGSNALVSTRNFPSGLEEANFPAVVSSPAEFRCVRVSSIDDADDRYAYQTAVNIGGHLFKGILYDFGPESSTNNNSNNSNYNNSNYMIGETSGEGGVGVAQPLNLITDSDTTVVASSGALVDPSSLYSAPINAFMTASGTQFFPRPRS*