AlfalfaGEDB | Msa1329040

M. sativa cultivar zhongmu-4 / Msa1329040

KEGG: -
GO: -
PFAM: PF12819.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329040	XP_013457476.1	84.225	748	111	4	1	747	104	845	0.0	1244

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329040	sp\|Q9LK35\|THE1_ARATH	43.536	758	370	18	2	723	105	840	0.0	546

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329040	A0A072UPA5	84.225	748	111	4	1	747	104	845	0.0	1244

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329040	MtrunA17_Chr4g0053571	84.225	748	111	4	1	747	104	845	0.0	1244
Msa1329040	MtrunA17_Chr4g0053561	74.567	751	123	7	1	747	102	788	0.0	1102
Msa1329040	MtrunA17_Chr4g0053521	74.124	742	165	9	1	724	660	1392	0.0	1043
Msa1329040	MtrunA17_Chr4g0053521	68.642	405	120	4	1	403	104	503	1.66e-170	527
Msa1329040	MtrunA17_Chr4g0053591	85.455	495	69	2	256	747	2	496	0.0	846
Msa1329040	MtrunA17_Chr2g0318301	43.240	747	378	13	2	724	105	829	0.0	560
Msa1329040	MtrunA17_Chr6g0468691	45.621	708	328	18	1	682	103	779	0.0	551
Msa1329040	MtrunA17_Chr4g0030441	43.576	755	363	16	2	732	111	826	0.0	549
Msa1329040	MtrunA17_Chr1g0202711	44.460	704	340	17	1	686	120	790	0.0	543
Msa1329040	MtrunA17_Chr8g0352381	44.380	694	347	15	2	678	137	808	9.41e-177	529

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329040	AT5G54380.1	43.536	758	370	18	2	723	105	840	0.0	546
Msa1329040	AT2G23200.1	44.602	704	339	14	1	687	111	780	0.0	542
Msa1329040	AT5G61350.1	43.733	718	342	16	2	678	112	808	0.0	540
Msa1329040	AT4G39110.1	46.264	696	332	16	2	678	131	803	9.24e-180	536
Msa1329040	AT2G21480.1	45.533	694	340	14	2	678	130	802	2.14e-178	533
Msa1329040	AT3G46290.1	44.751	724	354	17	14	720	113	807	3.54e-178	531
Msa1329040	AT5G24010.1	44.714	700	342	15	1	683	106	777	7.49e-176	525
Msa1329040	AT5G59700.1	44.928	690	336	15	2	677	100	759	8.22e-172	514
Msa1329040	AT3G51550.1	41.243	708	355	17	20	683	130	820	1.51e-155	474
Msa1329040	AT5G28680.1	42.170	728	342	23	1	678	101	799	1.58e-153	468

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Find 151 sgRNAs with CRISPR-Local

Find 360 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGTGTGCCGGGTTC+AGG	0.323584	8_4:-47186980	Msa1329040:CDS
AAACACCATCTTAATGTTGG+TGG	0.563153	8_4:-47187916	Msa1329040:CDS
AAAGAAGGTTAGTCTTCCTG+TGG	0.643977	8_4:-47187332	Msa1329040:CDS
AAATCAAAATGATAAGGAGC+TGG	0.555901	8_4:+47187502	None:intergenic
AAATCTGATGTTTATTCTTT+TGG	0.151712	8_4:-47186521	Msa1329040:CDS
AAATGTCATAAATGTAGAGA+AGG	0.572795	8_4:+47187569	None:intergenic
AAATTCAATGTTTCTGTTCC+TGG	0.279462	8_4:-47188207	Msa1329040:CDS
AACAATGGTTGATGTATTGT+GGG	0.543568	8_4:-47186279	Msa1329040:CDS
AACGCTAAGAATGGTTCCTT+TGG	0.414299	8_4:-47187817	Msa1329040:CDS
AAGAATATTTAGTGAGACTG+TGG	0.585982	8_4:-47186336	Msa1329040:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGAAGAGAATGAAAAGAAA+TGG	+	chr8_4:47186054-47186073	None:intergenic	30.0%
ATTTGGGGATTAAATTAACC+GGG	+	chr8_4:47186276-47186295	None:intergenic	30.0%
GATTTGGGGATTAAATTAAC+CGG	+	chr8_4:47186277-47186296	None:intergenic	30.0%
CTAATGGTTTAAACATAAGC+TGG	-	chr8_4:47186620-47186639	Msa1329040:CDS	30.0%
AAATCAAAATGATAAGGAGC+TGG	+	chr8_4:47186802-47186821	None:intergenic	30.0%
TTGGAATCCAAATCATCAAA+GGG	+	chr8_4:47186946-47186965	None:intergenic	30.0%
ATTGGAATCCAAATCATCAA+AGG	+	chr8_4:47186947-47186966	None:intergenic	30.0%
TTTGGATTCCAATTCAAAGA+AGG	-	chr8_4:47186954-47186973	Msa1329040:CDS	30.0%
AAGACTAACCTTCTTTGAAT+TGG	+	chr8_4:47186965-47186984	None:intergenic	30.0%
TCCCATAGCAAATTAACTTA+TGG	-	chr8_4:47187129-47187148	Msa1329040:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	47186040	47188283	47186040	ID=Msa1329040;Name=Msa1329040
chr8_4	mRNA	47186040	47188283	47186040	ID=Msa1329040-mRNA-1;Parent=Msa1329040;Name=Msa1329040-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|747
chr8_4	exon	47186040	47188283	47186040	ID=Msa1329040-mRNA-1:exon:13525;Parent=Msa1329040-mRNA-1
chr8_4	CDS	47186040	47188283	47186040	ID=Msa1329040-mRNA-1:cds;Parent=Msa1329040-mRNA-1

Gene Sequence

>Msa1329040

ATGTTACGTTTCCATTTCTTTTCATTCTCTTCACCACCTAAACTTTCCACTGCAAAATTCAATGTTTCTGTTCCTGGTTTCCTTCTGTTACAAAATTTCGATGCACAAAACACCACCAACTCTCCTTTAGTAAAAGAGTATTACGTTAAAGTCAACATAACCAGATTCAAAATCACTTTCACACCTCAAAAATCATCATTTGTGTTTGTTAACGCAATAGAACTCTTTATGCTCCCGGTTAATTTAATCCCCAAATCAGTCGTGCATTTCAATTACGCTGAATCTACTGGTCGAGGCTTATCTCCATACAACGGCGGTTTACACTCACAGCGAGCTTTGGAAACCAAACACCATCTTAATGTTGGTGGTGATACTGTCGACAGAGGAACAGATACTTTATCGAGACAATGGTTACCAGATCATATTTATATAACTAATCCACAAAACGCTAAGAATGGTTCCTTTGGAGGTGAAATTAAACGCACCGCAGATGATGAATCTGATTCTGATAATCAAGATTCAAATCAATATGTTGGTCCAGACAAGGTTTATGCAACTGCCCAAGAGACTATTAACGGCTCTAATGGTTTAAACATAAGCTGGAGTGTTCCTGTCGAGAAGAATATTGATCATTTCCTTAGGCTTCATTTTTGTGACATTTTTAATGACAGACAAAATGGTCTTACAGTATTCCTTCTCTACATTTATGACATTTATGTTTTGAATGTAAATGATGGCAGAGGCGTGTCATCCCAATTGCCAGCTCCTTATCATTTTGATTTTGTTGTTCGTTCAGATAACTCTGGGTTATTGAAAGTTACAATAATGCCTAATAAGACGGATTTTGTGCCGTACGCGTTTCTAAACGGGCTAGAAGTAATGAAAGTGTTTGAATCATCAGGTCCCTTTGATGATTTGGATTCCAATTCAAAGAAGGTTAGTCTTCCTGTGGTCGTGGGTTCGGTCGTTGGAGGTTTAGTTTTGGTTTCTGTTGTGATCGTTTTGTTTCTTTGGATAAGAAAAATCAGGAAACAAAGGCATGTTGAGAGTTTCGATTTGTTGCCGATTCCAACTGCTGCAGGAGGGATTTCCCATAGCAAATTAACTTATGGAAAAACAACTCAAGGCTCACCTATACCTAACATAAACCTTGGCATGAAAATCTCCTTGCTTGACCTTCAAATCGCCACGGAGAATTTCGATACGAAGAGGATTATTGGAAAGGGTGGTTTTGGAAATGTTTATAAGGGAGTTCTCAAGAATGGAATGAATGTGGCAGTGAAAAGAAGTGTGCCGGGTTCAGGTCAAGGACATCATGAATTTCAAACTGAGATAATGGTTTTGTCCAAGATTCGTCACATACACCTTGTTTCCTTAATAGGGTATTGTGATGAAAGGTGTGAGATGATACTTGTTTATGAGTACATGGAAAATGGTACACTTAGAGAGAATTTGTACAATACAAATTTGCCTTGTTTGACTTGGAAGCAAAGGCTTGAGATTTGCATTGGTGCTGCTAGAGGTCTTCATTACCTTCACAAAGGAGTAGCTGGTGGAATAATCCACCGTGATGTGAAATCCACCAATATATTGCTTGACGAGAATCTCGTAGCTAAAGTTGCTGATTTTGGTCTTTCAAGGACAGGTCCTCTTGATCAACATTCTTATGTCAGCACATGTGTTAAAGGGACTTTTGGGTATCTTGATCCTGATTACTTCAGATCACAACAGCTTACAGAAAAATCTGATGTTTATTCTTTTGGGGTGGTTCTACTTGAGGTGTTGTGCGCAAGACCAGCCATCGAATTGTCTTGTCCAAGTGAGCAAATCAATTTGGCTGAGTGGGGATTGCTTTGCAAAGACAAAGGGATACTTGAAGAAATCATTGATCCTTCCATCAAGGCACAAATTAATCAAAACTCACTAAGAATATTTAGTGAGACTGTGGAAAAATGCTTACACGAGTATGGTTGTGATAGGCCAACAATGGTTGATGTATTGTGGGACTTGGAATATGCTTTGCAGCTTCAGAGAGAACCACATGAGGATAGTTCTAGCAGTGCTTTTGCATCACTTCAATTGCCTAATGTTCAGCGTTTACCTTCTCTGTCTGCACTGACTGAAGTGGATGGCACGTCTACTGGGAGGGTTGATGAATCTGAAAGTGCAGCGTTGATGATGCTAGACGGAATTTCATGCGAAGCAAATCAAGGTACAACAATCAACACGCAAGTGTCTCCATGA

Protein sequence

>Msa1329040

MLRFHFFSFSSPPKLSTAKFNVSVPGFLLLQNFDAQNTTNSPLVKEYYVKVNITRFKITFTPQKSSFVFVNAIELFMLPVNLIPKSVVHFNYAESTGRGLSPYNGGLHSQRALETKHHLNVGGDTVDRGTDTLSRQWLPDHIYITNPQNAKNGSFGGEIKRTADDESDSDNQDSNQYVGPDKVYATAQETINGSNGLNISWSVPVEKNIDHFLRLHFCDIFNDRQNGLTVFLLYIYDIYVLNVNDGRGVSSQLPAPYHFDFVVRSDNSGLLKVTIMPNKTDFVPYAFLNGLEVMKVFESSGPFDDLDSNSKKVSLPVVVGSVVGGLVLVSVVIVLFLWIRKIRKQRHVESFDLLPIPTAAGGISHSKLTYGKTTQGSPIPNINLGMKISLLDLQIATENFDTKRIIGKGGFGNVYKGVLKNGMNVAVKRSVPGSGQGHHEFQTEIMVLSKIRHIHLVSLIGYCDERCEMILVYEYMENGTLRENLYNTNLPCLTWKQRLEICIGAARGLHYLHKGVAGGIIHRDVKSTNILLDENLVAKVADFGLSRTGPLDQHSYVSTCVKGTFGYLDPDYFRSQQLTEKSDVYSFGVVLLEVLCARPAIELSCPSEQINLAEWGLLCKDKGILEEIIDPSIKAQINQNSLRIFSETVEKCLHEYGCDRPTMVDVLWDLEYALQLQREPHEDSSSSAFASLQLPNVQRLPSLSALTEVDGTSTGRVDESESAALMMLDGISCEANQGTTINTQVSP*