Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1329780 | XP_013457865.1 | 98.997 | 299 | 3 | 0 | 1 | 299 | 1 | 299 | 0.0 | 620 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1329780 | sp|Q9STS6|LBD27_ARATH | 38.889 | 306 | 139 | 8 | 1 | 262 | 27 | 328 | 2.47e-54 | 182 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1329780 | A0A072URD2 | 98.997 | 299 | 3 | 0 | 1 | 299 | 1 | 299 | 0.0 | 620 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1329780 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1329780 | MtrunA17_Chr4g0059901 | 98.868 | 265 | 3 | 0 | 1 | 265 | 1 | 265 | 0.0 | 553 |
| Msa1329780 | MtrunA17_Chr8g0342351 | 58.333 | 108 | 45 | 0 | 7 | 114 | 9 | 116 | 1.39e-35 | 129 |
| Msa1329780 | MtrunA17_Chr6g0452671 | 51.786 | 112 | 54 | 0 | 4 | 115 | 5 | 116 | 2.60e-35 | 126 |
| Msa1329780 | MtrunA17_Chr6g0452661 | 50.000 | 114 | 56 | 1 | 9 | 121 | 8 | 121 | 1.23e-32 | 120 |
| Msa1329780 | MtrunA17_Chr6g0452691 | 49.138 | 116 | 58 | 1 | 7 | 121 | 6 | 121 | 7.21e-32 | 117 |
| Msa1329780 | MtrunA17_Chr2g0330201 | 51.376 | 109 | 53 | 0 | 6 | 114 | 15 | 123 | 3.71e-31 | 118 |
| Msa1329780 | MtrunA17_Chr5g0439111 | 51.304 | 115 | 55 | 1 | 9 | 122 | 8 | 122 | 1.64e-30 | 114 |
| Msa1329780 | MtrunA17_Chr7g0227511 | 37.580 | 157 | 86 | 4 | 11 | 162 | 12 | 161 | 5.71e-30 | 111 |
| Msa1329780 | MtrunA17_Chr3g0117121 | 50.538 | 93 | 46 | 0 | 11 | 103 | 11 | 103 | 1.35e-28 | 108 |
| Msa1329780 | MtrunA17_Chr4g0058651 | 48.958 | 96 | 49 | 0 | 11 | 106 | 12 | 107 | 1.88e-28 | 108 |
| Msa1329780 | MtrunA17_Chr4g0055441 | 47.222 | 108 | 56 | 1 | 10 | 116 | 2 | 109 | 2.07e-28 | 107 |
| Msa1329780 | MtrunA17_Chr4g0043421 | 48.000 | 100 | 52 | 0 | 4 | 103 | 9 | 108 | 3.36e-28 | 107 |
| Msa1329780 | MtrunA17_Chr5g0437571 | 43.220 | 118 | 66 | 1 | 6 | 123 | 2 | 118 | 1.04e-27 | 105 |
| Msa1329780 | MtrunA17_Chr6g0449651 | 44.545 | 110 | 61 | 0 | 1 | 110 | 58 | 167 | 1.54e-27 | 105 |
| Msa1329780 | MtrunA17_Chr8g0353981 | 38.037 | 163 | 98 | 1 | 11 | 173 | 8 | 167 | 2.51e-27 | 106 |
| Msa1329780 | MtrunA17_Chr6g0449641 | 47.000 | 100 | 53 | 0 | 11 | 110 | 10 | 109 | 3.02e-27 | 103 |
| Msa1329780 | MtrunA17_Chr3g0106101 | 43.478 | 115 | 64 | 1 | 5 | 119 | 2 | 115 | 4.78e-27 | 103 |
| Msa1329780 | MtrunA17_Chr2g0311601 | 46.000 | 100 | 54 | 0 | 4 | 103 | 7 | 106 | 8.06e-27 | 102 |
| Msa1329780 | MtrunA17_Chr3g0114061 | 42.857 | 119 | 67 | 1 | 3 | 121 | 1 | 118 | 1.35e-26 | 102 |
| Msa1329780 | MtrunA17_Chr5g0402611 | 43.363 | 113 | 64 | 0 | 11 | 123 | 9 | 121 | 7.98e-26 | 104 |
| Msa1329780 | MtrunA17_Chr3g0115431 | 45.000 | 100 | 55 | 0 | 11 | 110 | 10 | 109 | 1.07e-24 | 99.0 |
| Msa1329780 | MtrunA17_Chr5g0438091 | 41.346 | 104 | 61 | 0 | 7 | 110 | 36 | 139 | 7.71e-24 | 96.3 |
| Msa1329780 | MtrunA17_Chr4g0040831 | 39.474 | 114 | 62 | 1 | 6 | 119 | 11 | 117 | 8.44e-24 | 96.3 |
| Msa1329780 | MtrunA17_Chr1g0200201 | 37.121 | 132 | 71 | 1 | 11 | 130 | 73 | 204 | 3.42e-23 | 95.1 |
| Msa1329780 | MtrunA17_Chr7g0260971 | 41.509 | 106 | 61 | 1 | 6 | 110 | 10 | 115 | 6.99e-23 | 94.4 |
| Msa1329780 | MtrunA17_Chr3g0097221 | 45.000 | 100 | 55 | 0 | 11 | 110 | 8 | 107 | 1.39e-22 | 95.1 |
| Msa1329780 | MtrunA17_Chr4g0030001 | 43.617 | 94 | 52 | 1 | 11 | 103 | 25 | 118 | 2.25e-22 | 92.0 |
| Msa1329780 | MtrunA17_Chr5g0437401 | 38.318 | 107 | 66 | 0 | 7 | 113 | 8 | 114 | 6.27e-22 | 90.5 |
| Msa1329780 | MtrunA17_Chr5g0444351 | 40.909 | 110 | 64 | 1 | 11 | 120 | 6 | 114 | 9.20e-22 | 89.4 |
| Msa1329780 | MtrunA17_Chr5g0432451 | 43.269 | 104 | 59 | 0 | 7 | 110 | 40 | 143 | 4.56e-21 | 89.7 |
| Msa1329780 | MtrunA17_Chr7g0225111 | 41.000 | 100 | 59 | 0 | 11 | 110 | 6 | 105 | 5.09e-21 | 86.3 |
| Msa1329780 | MtrunA17_Chr3g0113891 | 40.206 | 97 | 58 | 0 | 7 | 103 | 11 | 107 | 5.22e-21 | 88.2 |
| Msa1329780 | MtrunA17_Chr3g0129071 | 38.462 | 104 | 64 | 0 | 7 | 110 | 44 | 147 | 7.62e-21 | 89.0 |
| Msa1329780 | MtrunA17_Chr8g0374591 | 38.462 | 104 | 64 | 0 | 7 | 110 | 5 | 108 | 2.08e-19 | 82.4 |
| Msa1329780 | MtrunA17_Chr6g0460951 | 41.584 | 101 | 58 | 1 | 11 | 110 | 41 | 141 | 2.73e-19 | 84.7 |
| Msa1329780 | MtrunA17_Chr8g0351721 | 40.385 | 104 | 57 | 2 | 5 | 103 | 9 | 112 | 4.98e-19 | 83.6 |
| Msa1329780 | MtrunA17_Chr5g0435641 | 38.384 | 99 | 60 | 1 | 6 | 103 | 3 | 101 | 6.34e-19 | 82.8 |
| Msa1329780 | MtrunA17_Chr8g0351701 | 41.489 | 94 | 54 | 1 | 11 | 103 | 24 | 117 | 1.51e-18 | 82.8 |
| Msa1329780 | MtrunA17_Chr1g0184281 | 41.000 | 100 | 58 | 1 | 11 | 109 | 16 | 115 | 4.71e-18 | 80.9 |
| Msa1329780 | MtrunA17_Chr8g0374601 | 38.235 | 102 | 63 | 0 | 9 | 110 | 25 | 126 | 1.25e-17 | 81.3 |
| Msa1329780 | MtrunA17_Chr1g0184271 | 39.000 | 100 | 60 | 1 | 11 | 109 | 13 | 112 | 1.58e-17 | 80.1 |
| Msa1329780 | MtrunA17_Chr7g0261031 | 37.143 | 105 | 65 | 1 | 6 | 109 | 4 | 108 | 3.34e-17 | 79.0 |
| Msa1329780 | MtrunA17_Chr7g0245871 | 39.362 | 94 | 56 | 1 | 11 | 103 | 28 | 121 | 3.51e-17 | 79.3 |
| Msa1329780 | MtrunA17_Chr6g0460941 | 41.379 | 87 | 50 | 1 | 11 | 96 | 8 | 94 | 2.52e-16 | 73.2 |
| Msa1329780 | MtrunA17_Chr8g0374581 | 38.835 | 103 | 62 | 1 | 9 | 110 | 7 | 109 | 1.38e-15 | 74.3 |
| Msa1329780 | MtrunA17_Chr2g0326041 | 52.083 | 48 | 23 | 0 | 11 | 58 | 34 | 81 | 1.36e-13 | 65.5 |
| Msa1329780 | MtrunA17_Chr4g0046201 | 33.333 | 87 | 58 | 0 | 10 | 96 | 6 | 92 | 4.87e-12 | 65.1 |
| Msa1329780 | MtrunA17_Chr6g0449311 | 33.083 | 133 | 86 | 2 | 38 | 167 | 1 | 133 | 8.34e-12 | 62.0 |
| Msa1329780 | MtrunA17_Chr7g0245861 | 38.202 | 89 | 52 | 2 | 11 | 96 | 13 | 101 | 3.10e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1329780 | AT3G47870.1 | 38.889 | 306 | 139 | 8 | 1 | 262 | 27 | 328 | 2.51e-55 | 182 |
| Msa1329780 | AT3G13850.1 | 46.452 | 155 | 68 | 2 | 9 | 161 | 35 | 176 | 1.07e-35 | 129 |
| Msa1329780 | AT1G72980.1 | 47.656 | 128 | 62 | 1 | 2 | 124 | 5 | 132 | 8.77e-34 | 123 |
| Msa1329780 | AT5G63090.4 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.70e-28 | 108 |
| Msa1329780 | AT5G63090.3 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.70e-28 | 108 |
| Msa1329780 | AT5G63090.2 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.70e-28 | 108 |
| Msa1329780 | AT5G63090.1 | 48.000 | 100 | 52 | 0 | 11 | 110 | 12 | 111 | 1.70e-28 | 108 |
| Msa1329780 | AT1G65620.1 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.11e-27 | 106 |
| Msa1329780 | AT1G65620.4 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.11e-27 | 106 |
| Msa1329780 | AT1G65620.3 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.11e-27 | 106 |
| Msa1329780 | AT1G65620.5 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.11e-27 | 106 |
| Msa1329780 | AT1G65620.2 | 55.422 | 83 | 37 | 0 | 7 | 89 | 6 | 88 | 1.11e-27 | 106 |
| Msa1329780 | AT3G27650.1 | 48.454 | 97 | 50 | 0 | 7 | 103 | 36 | 132 | 1.18e-27 | 105 |
| Msa1329780 | AT2G30130.1 | 41.667 | 120 | 69 | 1 | 3 | 122 | 1 | 119 | 2.09e-26 | 103 |
| Msa1329780 | AT5G66870.1 | 44.231 | 104 | 58 | 0 | 7 | 110 | 4 | 107 | 2.49e-25 | 103 |
| Msa1329780 | AT1G31320.1 | 47.312 | 93 | 49 | 0 | 4 | 96 | 7 | 99 | 2.52e-25 | 99.8 |
| Msa1329780 | AT2G28500.1 | 40.179 | 112 | 65 | 1 | 11 | 122 | 56 | 165 | 1.44e-22 | 94.0 |
| Msa1329780 | AT2G30340.2 | 40.909 | 110 | 63 | 1 | 7 | 116 | 50 | 157 | 1.90e-22 | 94.7 |
| Msa1329780 | AT2G30340.1 | 40.909 | 110 | 63 | 1 | 7 | 116 | 49 | 156 | 2.09e-22 | 94.4 |
| Msa1329780 | AT1G16530.1 | 41.284 | 109 | 61 | 2 | 5 | 112 | 9 | 115 | 2.85e-22 | 91.7 |
| Msa1329780 | AT1G07900.1 | 44.086 | 93 | 52 | 0 | 11 | 103 | 34 | 126 | 2.85e-22 | 92.0 |
| Msa1329780 | AT2G23660.2 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 7.27e-22 | 94.0 |
| Msa1329780 | AT2G23660.3 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 7.27e-22 | 94.0 |
| Msa1329780 | AT2G23660.1 | 42.000 | 100 | 58 | 0 | 11 | 110 | 6 | 105 | 7.27e-22 | 94.0 |
| Msa1329780 | AT3G11090.1 | 35.833 | 120 | 77 | 0 | 7 | 126 | 8 | 127 | 1.14e-21 | 89.7 |
| Msa1329780 | AT3G03760.1 | 41.584 | 101 | 58 | 1 | 11 | 110 | 52 | 152 | 1.01e-20 | 90.1 |
| Msa1329780 | AT1G06280.1 | 47.619 | 84 | 44 | 0 | 9 | 92 | 23 | 106 | 2.33e-20 | 87.4 |
| Msa1329780 | AT3G50510.1 | 42.000 | 100 | 58 | 0 | 11 | 110 | 13 | 112 | 2.43e-20 | 87.0 |
| Msa1329780 | AT3G50510.2 | 42.000 | 100 | 58 | 0 | 11 | 110 | 13 | 112 | 2.43e-20 | 87.0 |
| Msa1329780 | AT2G40470.2 | 39.655 | 116 | 70 | 0 | 7 | 122 | 17 | 132 | 5.58e-20 | 86.3 |
| Msa1329780 | AT2G40470.1 | 40.541 | 111 | 66 | 0 | 7 | 117 | 42 | 152 | 1.57e-19 | 85.5 |
| Msa1329780 | AT4G00210.1 | 39.623 | 106 | 63 | 1 | 6 | 110 | 7 | 112 | 5.60e-19 | 84.0 |
| Msa1329780 | AT5G06080.1 | 32.317 | 164 | 106 | 4 | 10 | 170 | 7 | 168 | 7.04e-19 | 82.8 |
| Msa1329780 | AT5G15060.1 | 45.570 | 79 | 41 | 1 | 5 | 81 | 12 | 90 | 7.35e-19 | 81.6 |
| Msa1329780 | AT3G58190.1 | 38.843 | 121 | 66 | 2 | 11 | 130 | 12 | 125 | 1.22e-18 | 83.2 |
| Msa1329780 | AT2G42440.1 | 35.537 | 121 | 70 | 2 | 6 | 125 | 3 | 116 | 1.56e-18 | 83.2 |
| Msa1329780 | AT3G26660.1 | 34.234 | 111 | 73 | 0 | 11 | 121 | 6 | 116 | 1.77e-18 | 80.1 |
| Msa1329780 | AT2G42430.1 | 36.792 | 106 | 66 | 1 | 6 | 110 | 11 | 116 | 3.61e-18 | 82.4 |
| Msa1329780 | AT4G00220.1 | 41.304 | 92 | 53 | 1 | 13 | 103 | 20 | 111 | 5.38e-18 | 81.6 |
| Msa1329780 | AT3G26620.1 | 33.036 | 112 | 75 | 0 | 11 | 122 | 6 | 117 | 5.38e-18 | 78.6 |
| Msa1329780 | AT2G45420.1 | 40.217 | 92 | 54 | 1 | 13 | 103 | 40 | 131 | 9.06e-18 | 81.6 |
| Msa1329780 | AT5G35900.1 | 28.906 | 128 | 86 | 1 | 11 | 133 | 6 | 133 | 2.35e-17 | 79.3 |
| Msa1329780 | AT2G31310.1 | 30.894 | 123 | 76 | 2 | 5 | 126 | 2 | 116 | 2.38e-16 | 76.3 |
| Msa1329780 | AT2G19820.1 | 33.673 | 98 | 59 | 2 | 11 | 107 | 13 | 105 | 7.90e-15 | 70.1 |
| Msa1329780 | AT2G45410.1 | 39.394 | 99 | 59 | 1 | 13 | 110 | 19 | 117 | 9.85e-15 | 71.6 |
Find 56 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGTCCACAAATTATT+TGG | 0.102603 | 8_4:-48638737 | Msa1329780:CDS |
| CAAATAATTTGTGGACATTT+TGG | 0.120261 | 8_4:+48638738 | None:intergenic |
| ATGCTACAAATCCTAATTTA+TGG | 0.149478 | 8_4:-48638277 | Msa1329780:CDS |
| CAGGTGTGCACTTTCTTCTT+TGG | 0.204290 | 8_4:+48638807 | None:intergenic |
| TTATCATTCAAGCTAATTAT+AGG | 0.238366 | 8_4:-48638646 | Msa1329780:CDS |
| GTGACTTCACAGTTAGAATT+AGG | 0.247178 | 8_4:-48638476 | Msa1329780:CDS |
| ATCAACAACTTGAAATTTGC+AGG | 0.281961 | 8_4:-48638535 | Msa1329780:CDS |
| GGTTGATCTGCAGGGAAATA+AGG | 0.290053 | 8_4:+48638770 | None:intergenic |
| GCAGGCTACAATATCAAATA+TGG | 0.297786 | 8_4:-48638586 | Msa1329780:CDS |
| GCAATCATACATTTATTCTA+AGG | 0.300515 | 8_4:-48638096 | Msa1329780:CDS |
| CTGCTATTATTATTTGTGTA+AGG | 0.327541 | 8_4:+48638245 | None:intergenic |
| TATCATTCAAGCTAATTATA+GGG | 0.334002 | 8_4:-48638645 | Msa1329780:CDS |
| CTTGTGTTGTGCCACCCTTA+AGG | 0.355961 | 8_4:+48638849 | None:intergenic |
| GGGCACTGAAGCCATAAATT+AGG | 0.357230 | 8_4:+48638266 | None:intergenic |
| TTGTGTTGTGCCACCCTTAA+GGG | 0.363348 | 8_4:+48638850 | None:intergenic |
| CAGGGAAATAAGGAGCTAAA+AGG | 0.379418 | 8_4:+48638780 | None:intergenic |
| TTGGAATTTGCATGCTGCAC+AGG | 0.387931 | 8_4:+48638826 | None:intergenic |
| TATCTTCAACAACTCCTTGT+TGG | 0.395214 | 8_4:+48638162 | None:intergenic |
| TGCTAACTCCAAATAATTTG+TGG | 0.405495 | 8_4:+48638729 | None:intergenic |
| AATAACAATAATGACCCTTA+AGG | 0.406316 | 8_4:-48638864 | None:intergenic |
| CATCATGAGGTAACTGTTGT+TGG | 0.408383 | 8_4:+48638501 | None:intergenic |
| ATAACAATAATGACCCTTAA+GGG | 0.428036 | 8_4:-48638863 | None:intergenic |
| TTGTTGTAACTCTGTGGTTG+TGG | 0.444565 | 8_4:+48638407 | None:intergenic |
| AAAGAGTGCGAGCGCATTGA+AGG | 0.447294 | 8_4:+48638439 | None:intergenic |
| GATGTTGTGAGGAAATTTGC+AGG | 0.467829 | 8_4:-48638604 | Msa1329780:CDS |
| ACAATATCAAATATGGATGA+TGG | 0.492612 | 8_4:-48638579 | Msa1329780:CDS |
| CTAAGGAGGCTTATGAATCA+AGG | 0.503681 | 8_4:-48638079 | Msa1329780:intron |
| TGCTATTATTATTTGTGTAA+GGG | 0.504837 | 8_4:+48638246 | None:intergenic |
| TCTGAGGTAACCAACTTATG+AGG | 0.517348 | 8_4:+48638206 | None:intergenic |
| ATAATCCACACTATTGCTGT+TGG | 0.518450 | 8_4:+48638334 | None:intergenic |
| AGCAATGCAACCTCATAAGT+TGG | 0.519249 | 8_4:-48638216 | Msa1329780:CDS |
| TAATAACTCCTCCCTCTACA+TGG | 0.525658 | 8_4:-48638312 | Msa1329780:CDS |
| TTGAATGATAATAGAATCCA+TGG | 0.528060 | 8_4:+48638658 | None:intergenic |
| CTCCCTCTACATGGATTCAA+AGG | 0.529912 | 8_4:-48638303 | Msa1329780:CDS |
| ATCATACATTTATTCTAAGG+AGG | 0.554320 | 8_4:-48638093 | Msa1329780:CDS |
| CTGATCAGTTGACACTGGTA+AGG | 0.557706 | 8_4:+48637757 | None:intergenic |
| TCCTCACAACATCCATGCAC+AGG | 0.573785 | 8_4:+48638614 | None:intergenic |
| CACACTATTGCTGTTGGAGT+AGG | 0.575688 | 8_4:+48638340 | None:intergenic |
| CCTTGTTGGACAGATGATAG+TGG | 0.578651 | 8_4:+48638176 | None:intergenic |
| AGGGATAAGTATCCTGTGCA+TGG | 0.583412 | 8_4:-48638626 | Msa1329780:CDS |
| TTCACAGTTAGAATTAGGAA+TGG | 0.586596 | 8_4:-48638471 | Msa1329780:CDS |
| ATCCTTTGAATCCATGTAGA+GGG | 0.590065 | 8_4:+48638301 | None:intergenic |
| TGTGAAAATACTAAAGTACC+TGG | 0.599565 | 8_4:-48638702 | Msa1329780:CDS |
| TTATTGTTGTTGTAACTCTG+TGG | 0.607863 | 8_4:+48638401 | None:intergenic |
| TAGTCAGAAGAAAACAGCCA+TGG | 0.610751 | 8_4:-48638675 | Msa1329780:CDS |
| TAAGGAGCTAAAAGGCACTC+AGG | 0.614015 | 8_4:+48638788 | None:intergenic |
| CTACTCCAACAGCAATAGTG+TGG | 0.620940 | 8_4:-48638339 | Msa1329780:CDS |
| CATCCTTTGAATCCATGTAG+AGG | 0.632560 | 8_4:+48638300 | None:intergenic |
| CCACTATCATCTGTCCAACA+AGG | 0.649871 | 8_4:-48638176 | Msa1329780:CDS |
| TAGTACTGATCAGTTGACAC+TGG | 0.662425 | 8_4:+48637752 | None:intergenic |
| CTTTGAATCCATGTAGAGGG+AGG | 0.666923 | 8_4:+48638304 | None:intergenic |
| TGATAGTGGCTGTGACTCTG+AGG | 0.678405 | 8_4:+48638190 | None:intergenic |
| TGTGAAGTCACATCATCATG+AGG | 0.679884 | 8_4:+48638488 | None:intergenic |
| ACAATAATGACCCTTAAGGG+TGG | 0.682430 | 8_4:-48638860 | None:intergenic |
| TCCTGTGCATGGATGTTGTG+AGG | 0.692042 | 8_4:-48638615 | Msa1329780:CDS |
| TGTTGCTGAGATACAGACAA+AGG | 0.703585 | 8_4:+48638365 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCATTCAAGCTAATTAT+AGG | - | chr8_4:48637969-48637988 | Msa1329780:intron | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | - | chr8_4:48637970-48637989 | Msa1329780:intron | 20.0% |
| !! | TGCTATTATTATTTGTGTAA+GGG | + | chr8_4:48638372-48638391 | None:intergenic | 20.0% |
| !! | AATGTTGAAATTAGATATAG+TGG | - | chr8_4:48638664-48638683 | Msa1329780:CDS | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | - | chr8_4:48638665-48638684 | Msa1329780:CDS | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | - | chr8_4:48637969-48637988 | Msa1329780:intron | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | - | chr8_4:48637970-48637989 | Msa1329780:intron | 20.0% |
| !! | TGCTATTATTATTTGTGTAA+GGG | + | chr8_4:48638372-48638391 | None:intergenic | 20.0% |
| !! | AATGTTGAAATTAGATATAG+TGG | - | chr8_4:48638664-48638683 | Msa1329780:CDS | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | - | chr8_4:48638665-48638684 | Msa1329780:CDS | 20.0% |
| !!! | TGGACATTTTGGAAAATTTT+GGG | + | chr8_4:48637869-48637888 | None:intergenic | 25.0% |
| !!! | CAAATAATTTGTGGACATTT+TGG | + | chr8_4:48637880-48637899 | None:intergenic | 25.0% |
| ! | CAAAATGTCCACAAATTATT+TGG | - | chr8_4:48637878-48637897 | Msa1329780:intron | 25.0% |
| ! | TTGAATGATAATAGAATCCA+TGG | + | chr8_4:48637960-48637979 | None:intergenic | 25.0% |
| ! | ACAATATCAAATATGGATGA+TGG | - | chr8_4:48638036-48638055 | Msa1329780:intron | 25.0% |
| ! | ATGCTACAAATCCTAATTTA+TGG | - | chr8_4:48638338-48638357 | Msa1329780:CDS | 25.0% |
| ! | CTGCTATTATTATTTGTGTA+AGG | + | chr8_4:48638373-48638392 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | - | chr8_4:48638497-48638516 | Msa1329780:CDS | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | - | chr8_4:48638519-48638538 | Msa1329780:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | - | chr8_4:48638522-48638541 | Msa1329780:CDS | 25.0% |
| !! | GTATTGTTTGATTTACTTGA+AGG | + | chr8_4:48638601-48638620 | None:intergenic | 25.0% |
| !!! | TGGACATTTTGGAAAATTTT+GGG | + | chr8_4:48637869-48637888 | None:intergenic | 25.0% |
| !!! | CAAATAATTTGTGGACATTT+TGG | + | chr8_4:48637880-48637899 | None:intergenic | 25.0% |
| ! | CAAAATGTCCACAAATTATT+TGG | - | chr8_4:48637878-48637897 | Msa1329780:intron | 25.0% |
| ! | TTGAATGATAATAGAATCCA+TGG | + | chr8_4:48637960-48637979 | None:intergenic | 25.0% |
| ! | ACAATATCAAATATGGATGA+TGG | - | chr8_4:48638036-48638055 | Msa1329780:intron | 25.0% |
| ! | ATGCTACAAATCCTAATTTA+TGG | - | chr8_4:48638338-48638357 | Msa1329780:CDS | 25.0% |
| ! | CTGCTATTATTATTTGTGTA+AGG | + | chr8_4:48638373-48638392 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | - | chr8_4:48638497-48638516 | Msa1329780:CDS | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | - | chr8_4:48638519-48638538 | Msa1329780:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | - | chr8_4:48638522-48638541 | Msa1329780:CDS | 25.0% |
| !! | GTATTGTTTGATTTACTTGA+AGG | + | chr8_4:48638601-48638620 | None:intergenic | 25.0% |
| !! | GTGGACATTTTGGAAAATTT+TGG | + | chr8_4:48637870-48637889 | None:intergenic | 30.0% |
| TGCTAACTCCAAATAATTTG+TGG | + | chr8_4:48637889-48637908 | None:intergenic | 30.0% | |
| ! | TGTGAAAATACTAAAGTACC+TGG | - | chr8_4:48637913-48637932 | Msa1329780:intron | 30.0% |
| ATCAACAACTTGAAATTTGC+AGG | - | chr8_4:48638080-48638099 | Msa1329780:CDS | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | - | chr8_4:48638144-48638163 | Msa1329780:CDS | 30.0% | |
| ! | TTATTGTTGTTGTAACTCTG+TGG | + | chr8_4:48638217-48638236 | None:intergenic | 30.0% |
| !! | GTGGACATTTTGGAAAATTT+TGG | + | chr8_4:48637870-48637889 | None:intergenic | 30.0% |
| TGCTAACTCCAAATAATTTG+TGG | + | chr8_4:48637889-48637908 | None:intergenic | 30.0% | |
| ! | TGTGAAAATACTAAAGTACC+TGG | - | chr8_4:48637913-48637932 | Msa1329780:intron | 30.0% |
| ATCAACAACTTGAAATTTGC+AGG | - | chr8_4:48638080-48638099 | Msa1329780:CDS | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | - | chr8_4:48638144-48638163 | Msa1329780:CDS | 30.0% | |
| ! | TTATTGTTGTTGTAACTCTG+TGG | + | chr8_4:48638217-48638236 | None:intergenic | 30.0% |
| !!! | AAATTTTGGGTTGATCTGCA+GGG | + | chr8_4:48637856-48637875 | None:intergenic | 35.0% |
| !!! | AAAATTTTGGGTTGATCTGC+AGG | + | chr8_4:48637857-48637876 | None:intergenic | 35.0% |
| GCAGGCTACAATATCAAATA+TGG | - | chr8_4:48638029-48638048 | Msa1329780:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | - | chr8_4:48638139-48638158 | Msa1329780:CDS | 35.0% | |
| ATAATCCACACTATTGCTGT+TGG | + | chr8_4:48638284-48638303 | None:intergenic | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | + | chr8_4:48638317-48638336 | None:intergenic | 35.0% | |
| TATCTTCAACAACTCCTTGT+TGG | + | chr8_4:48638456-48638475 | None:intergenic | 35.0% | |
| !!! | AAATTTTGGGTTGATCTGCA+GGG | + | chr8_4:48637856-48637875 | None:intergenic | 35.0% |
| !!! | AAAATTTTGGGTTGATCTGC+AGG | + | chr8_4:48637857-48637876 | None:intergenic | 35.0% |
| GCAGGCTACAATATCAAATA+TGG | - | chr8_4:48638029-48638048 | Msa1329780:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | - | chr8_4:48638139-48638158 | Msa1329780:CDS | 35.0% | |
| ATAATCCACACTATTGCTGT+TGG | + | chr8_4:48638284-48638303 | None:intergenic | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | + | chr8_4:48638317-48638336 | None:intergenic | 35.0% | |
| TATCTTCAACAACTCCTTGT+TGG | + | chr8_4:48638456-48638475 | None:intergenic | 35.0% | |
| CAGGGAAATAAGGAGCTAAA+AGG | + | chr8_4:48637838-48637857 | None:intergenic | 40.0% | |
| ! | TTTTCTTCTGACTAGGTTCC+AGG | + | chr8_4:48637934-48637953 | None:intergenic | 40.0% |
| !! | ATGGCTGTTTTCTTCTGACT+AGG | + | chr8_4:48637941-48637960 | None:intergenic | 40.0% |
| TAGTCAGAAGAAAACAGCCA+TGG | - | chr8_4:48637940-48637959 | Msa1329780:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | - | chr8_4:48638011-48638030 | Msa1329780:intron | 40.0% | |
| CATCATGAGGTAACTGTTGT+TGG | + | chr8_4:48638117-48638136 | None:intergenic | 40.0% | |
| TGTGAAGTCACATCATCATG+AGG | + | chr8_4:48638130-48638149 | None:intergenic | 40.0% | |
| TTGTTGTAACTCTGTGGTTG+TGG | + | chr8_4:48638211-48638230 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | + | chr8_4:48638253-48638272 | None:intergenic | 40.0% | |
| TAATAACTCCTCCCTCTACA+TGG | - | chr8_4:48638303-48638322 | Msa1329780:CDS | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | + | chr8_4:48638318-48638337 | None:intergenic | 40.0% | |
| AGCAATGCAACCTCATAAGT+TGG | - | chr8_4:48638399-48638418 | Msa1329780:CDS | 40.0% | |
| ! | TCTGAGGTAACCAACTTATG+AGG | + | chr8_4:48638412-48638431 | None:intergenic | 40.0% |
| CTAAGGAGGCTTATGAATCA+AGG | - | chr8_4:48638536-48638555 | Msa1329780:CDS | 40.0% | |
| CAGGGAAATAAGGAGCTAAA+AGG | + | chr8_4:48637838-48637857 | None:intergenic | 40.0% | |
| ! | TTTTCTTCTGACTAGGTTCC+AGG | + | chr8_4:48637934-48637953 | None:intergenic | 40.0% |
| !! | ATGGCTGTTTTCTTCTGACT+AGG | + | chr8_4:48637941-48637960 | None:intergenic | 40.0% |
| TAGTCAGAAGAAAACAGCCA+TGG | - | chr8_4:48637940-48637959 | Msa1329780:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | - | chr8_4:48638011-48638030 | Msa1329780:intron | 40.0% | |
| CATCATGAGGTAACTGTTGT+TGG | + | chr8_4:48638117-48638136 | None:intergenic | 40.0% | |
| TGTGAAGTCACATCATCATG+AGG | + | chr8_4:48638130-48638149 | None:intergenic | 40.0% | |
| TTGTTGTAACTCTGTGGTTG+TGG | + | chr8_4:48638211-48638230 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | + | chr8_4:48638253-48638272 | None:intergenic | 40.0% | |
| TAATAACTCCTCCCTCTACA+TGG | - | chr8_4:48638303-48638322 | Msa1329780:CDS | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | + | chr8_4:48638318-48638337 | None:intergenic | 40.0% | |
| AGCAATGCAACCTCATAAGT+TGG | - | chr8_4:48638399-48638418 | Msa1329780:CDS | 40.0% | |
| ! | TCTGAGGTAACCAACTTATG+AGG | + | chr8_4:48638412-48638431 | None:intergenic | 40.0% |
| CTAAGGAGGCTTATGAATCA+AGG | - | chr8_4:48638536-48638555 | Msa1329780:CDS | 40.0% | |
| TTGTGTTGTGCCACCCTTAA+GGG | + | chr8_4:48637768-48637787 | None:intergenic | 45.0% | |
| TTGGAATTTGCATGCTGCAC+AGG | + | chr8_4:48637792-48637811 | None:intergenic | 45.0% | |
| CAGGTGTGCACTTTCTTCTT+TGG | + | chr8_4:48637811-48637830 | None:intergenic | 45.0% | |
| !! | TAAGGAGCTAAAAGGCACTC+AGG | + | chr8_4:48637830-48637849 | None:intergenic | 45.0% |
| GGTTGATCTGCAGGGAAATA+AGG | + | chr8_4:48637848-48637867 | None:intergenic | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | - | chr8_4:48637989-48638008 | Msa1329780:intron | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | + | chr8_4:48638278-48638297 | None:intergenic | 45.0% |
| CTACTCCAACAGCAATAGTG+TGG | - | chr8_4:48638276-48638295 | Msa1329780:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | + | chr8_4:48638314-48638333 | None:intergenic | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | - | chr8_4:48638312-48638331 | Msa1329780:CDS | 45.0% | |
| !! | GGGCACTGAAGCCATAAATT+AGG | + | chr8_4:48638352-48638371 | None:intergenic | 45.0% |
| CCTTGTTGGACAGATGATAG+TGG | + | chr8_4:48638442-48638461 | None:intergenic | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | - | chr8_4:48638439-48638458 | Msa1329780:CDS | 45.0% | |
| GAACTGCACGCAGAAATGTA+CGG | + | chr8_4:48638757-48638776 | None:intergenic | 45.0% | |
| GTACATTTCTGCGTGCAGTT+CGG | - | chr8_4:48638756-48638775 | Msa1329780:CDS | 45.0% | |
| TTGTGTTGTGCCACCCTTAA+GGG | + | chr8_4:48637768-48637787 | None:intergenic | 45.0% | |
| TTGGAATTTGCATGCTGCAC+AGG | + | chr8_4:48637792-48637811 | None:intergenic | 45.0% | |
| CAGGTGTGCACTTTCTTCTT+TGG | + | chr8_4:48637811-48637830 | None:intergenic | 45.0% | |
| !! | TAAGGAGCTAAAAGGCACTC+AGG | + | chr8_4:48637830-48637849 | None:intergenic | 45.0% |
| GGTTGATCTGCAGGGAAATA+AGG | + | chr8_4:48637848-48637867 | None:intergenic | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | - | chr8_4:48637989-48638008 | Msa1329780:intron | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | + | chr8_4:48638278-48638297 | None:intergenic | 45.0% |
| CTACTCCAACAGCAATAGTG+TGG | - | chr8_4:48638276-48638295 | Msa1329780:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | + | chr8_4:48638314-48638333 | None:intergenic | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | - | chr8_4:48638312-48638331 | Msa1329780:CDS | 45.0% | |
| !! | GGGCACTGAAGCCATAAATT+AGG | + | chr8_4:48638352-48638371 | None:intergenic | 45.0% |
| CCTTGTTGGACAGATGATAG+TGG | + | chr8_4:48638442-48638461 | None:intergenic | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | - | chr8_4:48638439-48638458 | Msa1329780:CDS | 45.0% | |
| GAACTGCACGCAGAAATGTA+CGG | + | chr8_4:48638757-48638776 | None:intergenic | 45.0% | |
| GTACATTTCTGCGTGCAGTT+CGG | - | chr8_4:48638756-48638775 | Msa1329780:CDS | 45.0% | |
| CTTGTGTTGTGCCACCCTTA+AGG | + | chr8_4:48637769-48637788 | None:intergenic | 50.0% | |
| TCCTGTGCATGGATGTTGTG+AGG | - | chr8_4:48638000-48638019 | Msa1329780:intron | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | + | chr8_4:48638004-48638023 | None:intergenic | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | + | chr8_4:48638179-48638198 | None:intergenic | 50.0% |
| !! | TGATAGTGGCTGTGACTCTG+AGG | + | chr8_4:48638428-48638447 | None:intergenic | 50.0% |
| CTTGTGTTGTGCCACCCTTA+AGG | + | chr8_4:48637769-48637788 | None:intergenic | 50.0% | |
| TCCTGTGCATGGATGTTGTG+AGG | - | chr8_4:48638000-48638019 | Msa1329780:intron | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | + | chr8_4:48638004-48638023 | None:intergenic | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | + | chr8_4:48638179-48638198 | None:intergenic | 50.0% |
| !! | TGATAGTGGCTGTGACTCTG+AGG | + | chr8_4:48638428-48638447 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 48637761 | 48638876 | 48637761 | ID=Msa1329780;Name=Msa1329780 |
| chr8_4 | mRNA | 48637761 | 48638876 | 48637761 | ID=Msa1329780-mRNA-1;Parent=Msa1329780;Name=Msa1329780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|299 |
| chr8_4 | exon | 48638080 | 48638876 | 48638080 | ID=Msa1329780-mRNA-1:exon:13989;Parent=Msa1329780-mRNA-1 |
| chr8_4 | exon | 48637761 | 48637863 | 48637761 | ID=Msa1329780-mRNA-1:exon:13988;Parent=Msa1329780-mRNA-1 |
| chr8_4 | CDS | 48638080 | 48638876 | 48638080 | ID=Msa1329780-mRNA-1:cds;Parent=Msa1329780-mRNA-1 |
| chr8_4 | CDS | 48637761 | 48637863 | 48637761 | ID=Msa1329780-mRNA-1:cds;Parent=Msa1329780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
