AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1330040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330040 MtrunA17_Chr4g0063371 82.906 117 13 1 1 117 116 225 2.16e-57 177
Msa1330040 MtrunA17_Chr4g0063391 53.763 93 13 1 1 63 118 210 8.57e-27 99.0
Msa1330040 MtrunA17_Chr4g0063401 42.718 103 17 2 1 62 147 248 7.40e-18 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330040 AT3G19360.1 30.055 183 61 4 1 117 186 367 2.93e-19 81.6
Msa1330040 AT3G19360.2 30.055 183 61 4 1 117 186 367 2.93e-19 81.6

Find 35 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGATCCTGCACAATTT+AGG 0.163729 8_4:+49073543 Msa1330040:CDS
CCAAGTATACGTTTCTTCTT+AGG 0.214369 8_4:-49074541 None:intergenic
TGAAGATCCTGCACAATTTA+GGG 0.278155 8_4:+49073544 Msa1330040:CDS
AGTCAGATCAATCGTATTTA+CGG 0.310380 8_4:+49074574 Msa1330040:CDS
GATACAGGATATTGTTCCTT+TGG 0.336419 8_4:+49073605 Msa1330040:CDS
CACAATTTAGGGGCACTTAT+CGG 0.370314 8_4:+49073555 Msa1330040:CDS
AATTGCAGCTTCAGCTTCAA+TGG 0.390999 8_4:-49074449 None:intergenic
AAATCAGAGTTACAAGTTCC+TGG 0.400098 8_4:+49074421 Msa1330040:intron
GACTTGCAGGGGAGGTGGTA+GGG 0.419543 8_4:-49074492 None:intergenic
TGCAAGTCAACTTGTGGAAA+AGG 0.437938 8_4:+49074507 Msa1330040:CDS
CCTAAGAAGAAACGTATACT+TGG 0.441257 8_4:+49074541 Msa1330040:CDS
CACTTTGCTCATGGTGAAGA+AGG 0.463092 8_4:+49073638 Msa1330040:CDS
TGTCTCAAGTGGAAGGATAC+AGG 0.464303 8_4:+49073590 Msa1330040:CDS
TGTGCAAGAAGTATAGCAAT+GGG 0.488339 8_4:+49073483 Msa1330040:CDS
TGACTTGCAGGGGAGGTGGT+AGG 0.490726 8_4:-49074493 None:intergenic
CACAAGTTGACTTGCAGGGG+AGG 0.509364 8_4:-49074500 None:intergenic
AATTTACACTTATCTGCATA+GGG 0.538027 8_4:-49073515 None:intergenic
CTCCCCTGCAAGTCAACTTG+TGG 0.543090 8_4:+49074501 Msa1330040:CDS
AAGTTGACTTGCAGGGGAGG+TGG 0.548779 8_4:-49074497 None:intergenic
GAATTTACACTTATCTGCAT+AGG 0.559420 8_4:-49073516 None:intergenic
TTTCCACAAGTTGACTTGCA+GGG 0.560920 8_4:-49074504 None:intergenic
TAAGTGCCCCTAAATTGTGC+AGG 0.568865 8_4:-49073551 None:intergenic
CTGTGCAAGAAGTATAGCAA+TGG 0.572239 8_4:+49073482 Msa1330040:CDS
TCAGCTTCAATGGCTCCACC+AGG 0.575223 8_4:-49074439 None:intergenic
CTAAGAAGAAACGTATACTT+GGG 0.583825 8_4:+49074542 Msa1330040:CDS
AAGAACTGTCACTTTGCTCA+TGG 0.585017 8_4:+49073629 Msa1330040:CDS
TTCCACAAGTTGACTTGCAG+GGG 0.588906 8_4:-49074503 None:intergenic
AGACTAAGCTGTGTCTCAAG+TGG 0.589149 8_4:+49073579 Msa1330040:CDS
ATTGTGCAGGATCTTCATGA+AGG 0.599081 8_4:-49073538 None:intergenic
TAAGCTGTGTCTCAAGTGGA+AGG 0.609632 8_4:+49073583 Msa1330040:CDS
AAAGTGACAGTTCTTTCCAA+AGG 0.613225 8_4:-49073621 None:intergenic
GAAGATCCTGCACAATTTAG+GGG 0.650410 8_4:+49073545 Msa1330040:CDS
CAAGAAGTATAGCAATGGGG+AGG 0.680526 8_4:+49073487 Msa1330040:CDS
TCAGAGTTACAAGTTCCTGG+TGG 0.718686 8_4:+49074424 Msa1330040:intron
GTGCAAGAAGTATAGCAATG+GGG 0.763140 8_4:+49073484 Msa1330040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAAGTTTTACATT+TGG + chr8_4:49073951-49073970 Msa1330040:intron 10.0%
!!! GACAATTTTTTTTAAATATA+AGG - chr8_4:49074272-49074291 None:intergenic 10.0%
!!! AAAAAAAAAAGTTTTACATT+TGG + chr8_4:49073951-49073970 Msa1330040:intron 10.0%
!!! GACAATTTTTTTTAAATATA+AGG - chr8_4:49074272-49074291 None:intergenic 10.0%
!!! TTTTTTTAAATATAAGGTCT+AGG - chr8_4:49074266-49074285 None:intergenic 15.0%
!!! TTTTTTTAAATATAAGGTCT+AGG - chr8_4:49074266-49074285 None:intergenic 15.0%
!!! CAATAACTCATTTTGATTAT+TGG + chr8_4:49073664-49073683 Msa1330040:intron 20.0%
!!! TACTATAATTTCTAGTAGTT+TGG + chr8_4:49073979-49073998 Msa1330040:intron 20.0%
!!! TTATAACAATTGCAACTTTT+TGG + chr8_4:49074353-49074372 Msa1330040:intron 20.0%
!!! CAATAACTCATTTTGATTAT+TGG + chr8_4:49073664-49073683 Msa1330040:intron 20.0%
!!! TACTATAATTTCTAGTAGTT+TGG + chr8_4:49073979-49073998 Msa1330040:intron 20.0%
!!! TTATAACAATTGCAACTTTT+TGG + chr8_4:49074353-49074372 Msa1330040:intron 20.0%
! AATTTACACTTATCTGCATA+GGG - chr8_4:49073518-49073537 None:intergenic 25.0%
!!! CTCATTTTGATTATTGGTTT+TGG + chr8_4:49073670-49073689 Msa1330040:intron 25.0%
! AGTAATCATCCATTAGAATA+AGG - chr8_4:49073910-49073929 None:intergenic 25.0%
! AATGGATGATTACTATTGTA+TGG + chr8_4:49073916-49073935 Msa1330040:intron 25.0%
!! ACTTTAAGAAGAAACAAGTT+TGG - chr8_4:49074054-49074073 None:intergenic 25.0%
!! TTAAAGTTTCTGCTGATTAT+TGG + chr8_4:49074067-49074086 Msa1330040:intron 25.0%
! AATTTACACTTATCTGCATA+GGG - chr8_4:49073518-49073537 None:intergenic 25.0%
!!! CTCATTTTGATTATTGGTTT+TGG + chr8_4:49073670-49073689 Msa1330040:intron 25.0%
! AGTAATCATCCATTAGAATA+AGG - chr8_4:49073910-49073929 None:intergenic 25.0%
! AATGGATGATTACTATTGTA+TGG + chr8_4:49073916-49073935 Msa1330040:intron 25.0%
!! ACTTTAAGAAGAAACAAGTT+TGG - chr8_4:49074054-49074073 None:intergenic 25.0%
!! TTAAAGTTTCTGCTGATTAT+TGG + chr8_4:49074067-49074086 Msa1330040:intron 25.0%
GAATTTACACTTATCTGCAT+AGG - chr8_4:49073519-49073538 None:intergenic 30.0%
!!! TTTGAATGTGTGTCATTTTG+TGG + chr8_4:49073818-49073837 Msa1330040:intron 30.0%
TGATTCCATCCTTATTCTAA+TGG + chr8_4:49073898-49073917 Msa1330040:intron 30.0%
ATCATCCATTAGAATAAGGA+TGG - chr8_4:49073906-49073925 None:intergenic 30.0%
GATGAACATAATACAATAGC+TGG - chr8_4:49074027-49074046 None:intergenic 30.0%
CCTCAAAATTATTGATGAGT+CGG - chr8_4:49074108-49074127 None:intergenic 30.0%
AAGAGTCAGAACAATATACA+TGG - chr8_4:49074382-49074401 None:intergenic 30.0%
!!! AGGGTTTGTTGAATTTTCTA+TGG - chr8_4:49074476-49074495 None:intergenic 30.0%
CTAAGAAGAAACGTATACTT+GGG + chr8_4:49074542-49074561 Msa1330040:CDS 30.0%
GAATTTACACTTATCTGCAT+AGG - chr8_4:49073519-49073538 None:intergenic 30.0%
!!! TTTGAATGTGTGTCATTTTG+TGG + chr8_4:49073818-49073837 Msa1330040:intron 30.0%
TGATTCCATCCTTATTCTAA+TGG + chr8_4:49073898-49073917 Msa1330040:intron 30.0%
ATCATCCATTAGAATAAGGA+TGG - chr8_4:49073906-49073925 None:intergenic 30.0%
GATGAACATAATACAATAGC+TGG - chr8_4:49074027-49074046 None:intergenic 30.0%
CCTCAAAATTATTGATGAGT+CGG - chr8_4:49074108-49074127 None:intergenic 30.0%
AAGAGTCAGAACAATATACA+TGG - chr8_4:49074382-49074401 None:intergenic 30.0%
!!! AGGGTTTGTTGAATTTTCTA+TGG - chr8_4:49074476-49074495 None:intergenic 30.0%
CTAAGAAGAAACGTATACTT+GGG + chr8_4:49074542-49074561 Msa1330040:CDS 30.0%
! TGTGCAAGAAGTATAGCAAT+GGG + chr8_4:49073483-49073502 Msa1330040:CDS 35.0%
ATGAAGATCCTGCACAATTT+AGG + chr8_4:49073543-49073562 Msa1330040:CDS 35.0%
TGAAGATCCTGCACAATTTA+GGG + chr8_4:49073544-49073563 Msa1330040:CDS 35.0%
GATACAGGATATTGTTCCTT+TGG + chr8_4:49073605-49073624 Msa1330040:CDS 35.0%
AAAGTGACAGTTCTTTCCAA+AGG - chr8_4:49073624-49073643 None:intergenic 35.0%
!! TTGACAAAGCAAGTTCTGAA+TGG + chr8_4:49073871-49073890 Msa1330040:intron 35.0%
! CCGACTCATCAATAATTTTG+AGG + chr8_4:49074105-49074124 Msa1330040:intron 35.0%
CTTATTTGACCGTGGTATTA+AGG - chr8_4:49074176-49074195 None:intergenic 35.0%
CTTAATACCACGGTCAAATA+AGG + chr8_4:49074174-49074193 Msa1330040:intron 35.0%
TGTCTTCATCATCACAAATG+CGG - chr8_4:49074209-49074228 None:intergenic 35.0%
ATCATACACTTGCACACAAA+TGG - chr8_4:49074294-49074313 None:intergenic 35.0%
TGTTCTGACTCTTGAAGTAT+TGG + chr8_4:49074389-49074408 Msa1330040:intron 35.0%
AAATCAGAGTTACAAGTTCC+TGG + chr8_4:49074421-49074440 Msa1330040:intron 35.0%
CCAAGTATACGTTTCTTCTT+AGG - chr8_4:49074544-49074563 None:intergenic 35.0%
CCTAAGAAGAAACGTATACT+TGG + chr8_4:49074541-49074560 Msa1330040:CDS 35.0%
! TGTGCAAGAAGTATAGCAAT+GGG + chr8_4:49073483-49073502 Msa1330040:CDS 35.0%
ATGAAGATCCTGCACAATTT+AGG + chr8_4:49073543-49073562 Msa1330040:CDS 35.0%
TGAAGATCCTGCACAATTTA+GGG + chr8_4:49073544-49073563 Msa1330040:CDS 35.0%
GATACAGGATATTGTTCCTT+TGG + chr8_4:49073605-49073624 Msa1330040:CDS 35.0%
AAAGTGACAGTTCTTTCCAA+AGG - chr8_4:49073624-49073643 None:intergenic 35.0%
!! TTGACAAAGCAAGTTCTGAA+TGG + chr8_4:49073871-49073890 Msa1330040:intron 35.0%
! CCGACTCATCAATAATTTTG+AGG + chr8_4:49074105-49074124 Msa1330040:intron 35.0%
CTTATTTGACCGTGGTATTA+AGG - chr8_4:49074176-49074195 None:intergenic 35.0%
CTTAATACCACGGTCAAATA+AGG + chr8_4:49074174-49074193 Msa1330040:intron 35.0%
TGTCTTCATCATCACAAATG+CGG - chr8_4:49074209-49074228 None:intergenic 35.0%
ATCATACACTTGCACACAAA+TGG - chr8_4:49074294-49074313 None:intergenic 35.0%
TGTTCTGACTCTTGAAGTAT+TGG + chr8_4:49074389-49074408 Msa1330040:intron 35.0%
AAATCAGAGTTACAAGTTCC+TGG + chr8_4:49074421-49074440 Msa1330040:intron 35.0%
CCAAGTATACGTTTCTTCTT+AGG - chr8_4:49074544-49074563 None:intergenic 35.0%
CCTAAGAAGAAACGTATACT+TGG + chr8_4:49074541-49074560 Msa1330040:CDS 35.0%
! CTGTGCAAGAAGTATAGCAA+TGG + chr8_4:49073482-49073501 Msa1330040:CDS 40.0%
! GTGCAAGAAGTATAGCAATG+GGG + chr8_4:49073484-49073503 Msa1330040:CDS 40.0%
ATTGTGCAGGATCTTCATGA+AGG - chr8_4:49073541-49073560 None:intergenic 40.0%
GAAGATCCTGCACAATTTAG+GGG + chr8_4:49073545-49073564 Msa1330040:CDS 40.0%
!! CACAATTTAGGGGCACTTAT+CGG + chr8_4:49073555-49073574 Msa1330040:CDS 40.0%
!! AAGAACTGTCACTTTGCTCA+TGG + chr8_4:49073629-49073648 Msa1330040:CDS 40.0%
! GCATTAACCTTATTTGACCG+TGG - chr8_4:49074184-49074203 None:intergenic 40.0%
AATTGCAGCTTCAGCTTCAA+TGG - chr8_4:49074452-49074471 None:intergenic 40.0%
!! TTTCCACAAGTTGACTTGCA+GGG - chr8_4:49074507-49074526 None:intergenic 40.0%
!!! TTTTCCACAAGTTGACTTGC+AGG - chr8_4:49074508-49074527 None:intergenic 40.0%
TGCAAGTCAACTTGTGGAAA+AGG + chr8_4:49074507-49074526 Msa1330040:CDS 40.0%
! CTGTGCAAGAAGTATAGCAA+TGG + chr8_4:49073482-49073501 Msa1330040:CDS 40.0%
! GTGCAAGAAGTATAGCAATG+GGG + chr8_4:49073484-49073503 Msa1330040:CDS 40.0%
ATTGTGCAGGATCTTCATGA+AGG - chr8_4:49073541-49073560 None:intergenic 40.0%
GAAGATCCTGCACAATTTAG+GGG + chr8_4:49073545-49073564 Msa1330040:CDS 40.0%
!! CACAATTTAGGGGCACTTAT+CGG + chr8_4:49073555-49073574 Msa1330040:CDS 40.0%
!! AAGAACTGTCACTTTGCTCA+TGG + chr8_4:49073629-49073648 Msa1330040:CDS 40.0%
! GCATTAACCTTATTTGACCG+TGG - chr8_4:49074184-49074203 None:intergenic 40.0%
AATTGCAGCTTCAGCTTCAA+TGG - chr8_4:49074452-49074471 None:intergenic 40.0%
!! TTTCCACAAGTTGACTTGCA+GGG - chr8_4:49074507-49074526 None:intergenic 40.0%
!!! TTTTCCACAAGTTGACTTGC+AGG - chr8_4:49074508-49074527 None:intergenic 40.0%
TGCAAGTCAACTTGTGGAAA+AGG + chr8_4:49074507-49074526 Msa1330040:CDS 40.0%
! CAAGAAGTATAGCAATGGGG+AGG + chr8_4:49073487-49073506 Msa1330040:CDS 45.0%
TAAGTGCCCCTAAATTGTGC+AGG - chr8_4:49073554-49073573 None:intergenic 45.0%
AGACTAAGCTGTGTCTCAAG+TGG + chr8_4:49073579-49073598 Msa1330040:CDS 45.0%
TAAGCTGTGTCTCAAGTGGA+AGG + chr8_4:49073583-49073602 Msa1330040:CDS 45.0%
TGTCTCAAGTGGAAGGATAC+AGG + chr8_4:49073590-49073609 Msa1330040:CDS 45.0%
CACTTTGCTCATGGTGAAGA+AGG + chr8_4:49073638-49073657 Msa1330040:CDS 45.0%
TGGTATTAAGGATCGTGGCA+TGG - chr8_4:49074164-49074183 None:intergenic 45.0%
TGCCACGATCCTTAATACCA+CGG + chr8_4:49074164-49074183 Msa1330040:intron 45.0%
TCAGAGTTACAAGTTCCTGG+TGG + chr8_4:49074424-49074443 Msa1330040:intron 45.0%
!! TTCCACAAGTTGACTTGCAG+GGG - chr8_4:49074506-49074525 None:intergenic 45.0%
! CAAGAAGTATAGCAATGGGG+AGG + chr8_4:49073487-49073506 Msa1330040:CDS 45.0%
TAAGTGCCCCTAAATTGTGC+AGG - chr8_4:49073554-49073573 None:intergenic 45.0%
AGACTAAGCTGTGTCTCAAG+TGG + chr8_4:49073579-49073598 Msa1330040:CDS 45.0%
TAAGCTGTGTCTCAAGTGGA+AGG + chr8_4:49073583-49073602 Msa1330040:CDS 45.0%
TGTCTCAAGTGGAAGGATAC+AGG + chr8_4:49073590-49073609 Msa1330040:CDS 45.0%
CACTTTGCTCATGGTGAAGA+AGG + chr8_4:49073638-49073657 Msa1330040:CDS 45.0%
TGGTATTAAGGATCGTGGCA+TGG - chr8_4:49074164-49074183 None:intergenic 45.0%
TGCCACGATCCTTAATACCA+CGG + chr8_4:49074164-49074183 Msa1330040:intron 45.0%
TCAGAGTTACAAGTTCCTGG+TGG + chr8_4:49074424-49074443 Msa1330040:intron 45.0%
!! TTCCACAAGTTGACTTGCAG+GGG - chr8_4:49074506-49074525 None:intergenic 45.0%
GACCGTGGTATTAAGGATCG+TGG - chr8_4:49074169-49074188 None:intergenic 50.0%
GACCGTGGTATTAAGGATCG+TGG - chr8_4:49074169-49074188 None:intergenic 50.0%
! AAGGTCTAGGACCACGACTG+TGG - chr8_4:49074253-49074272 None:intergenic 55.0%
TCAGCTTCAATGGCTCCACC+AGG - chr8_4:49074442-49074461 None:intergenic 55.0%
!! AAGTTGACTTGCAGGGGAGG+TGG - chr8_4:49074500-49074519 None:intergenic 55.0%
!! CACAAGTTGACTTGCAGGGG+AGG - chr8_4:49074503-49074522 None:intergenic 55.0%
CTCCCCTGCAAGTCAACTTG+TGG + chr8_4:49074501-49074520 Msa1330040:CDS 55.0%
! AAGGTCTAGGACCACGACTG+TGG - chr8_4:49074253-49074272 None:intergenic 55.0%
TCAGCTTCAATGGCTCCACC+AGG - chr8_4:49074442-49074461 None:intergenic 55.0%
!! AAGTTGACTTGCAGGGGAGG+TGG - chr8_4:49074500-49074519 None:intergenic 55.0%
!! CACAAGTTGACTTGCAGGGG+AGG - chr8_4:49074503-49074522 None:intergenic 55.0%
CTCCCCTGCAAGTCAACTTG+TGG + chr8_4:49074501-49074520 Msa1330040:CDS 55.0%
GTACGTCTCAGCCACAGTCG+TGG + chr8_4:49074239-49074258 Msa1330040:intron 60.0%
GACTTGCAGGGGAGGTGGTA+GGG - chr8_4:49074495-49074514 None:intergenic 60.0%
! TGACTTGCAGGGGAGGTGGT+AGG - chr8_4:49074496-49074515 None:intergenic 60.0%
GTACGTCTCAGCCACAGTCG+TGG + chr8_4:49074239-49074258 Msa1330040:intron 60.0%
GACTTGCAGGGGAGGTGGTA+GGG - chr8_4:49074495-49074514 None:intergenic 60.0%
! TGACTTGCAGGGGAGGTGGT+AGG - chr8_4:49074496-49074515 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 49073476 49074594 49073476 ID=Msa1330040;Name=Msa1330040
chr8_4 mRNA 49073476 49074594 49073476 ID=Msa1330040-mRNA-1;Parent=Msa1330040;Name=Msa1330040-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|0|2|0|117
chr8_4 exon 49073476 49073659 49073476 ID=Msa1330040-mRNA-1:exon:14142;Parent=Msa1330040-mRNA-1
chr8_4 exon 49074428 49074594 49074428 ID=Msa1330040-mRNA-1:exon:14143;Parent=Msa1330040-mRNA-1
chr8_4 CDS 49073476 49073659 49073476 ID=Msa1330040-mRNA-1:cds;Parent=Msa1330040-mRNA-1
chr8_4 CDS 49074428 49074594 49074428 ID=Msa1330040-mRNA-1:cds;Parent=Msa1330040-mRNA-1
Gene Sequence

>Msa1330040

ATGAAACTGTGCAAGAAGTATAGCAATGGGGAGGAATGTCCCTATGCAGATAAGTGTAAATTCCTTCATGAAGATCCTGCACAATTTAGGGGCACTTATCGGAAGACTAAGCTGTGTCTCAAGTGGAAGGATACAGGATATTGTTCCTTTGGAAAGAACTGTCACTTTGCTCATGGTGAAGAAGAGTTACAAGTTCCTGGTGGAGCCATTGAAGCTGAAGCTGCAATTACCATAGAAAATTCAACAAACCCTACCACCTCCCCTGCAAGTCAACTTGTGGAAAAGGAAGAAGAGCAGCCTAAGAAGAAACGTATACTTGGGTTGAAATTCAGTCAGATCAATCGTATTTAC

Protein sequence

>Msa1330040

MKLCKKYSNGEECPYADKCKFLHEDPAQFRGTYRKTKLCLKWKDTGYCSFGKNCHFAHGEEELQVPGGAIEAEAAITIENSTNPTTSPASQLVEKEEEQPKKKRILGLKFSQINRIY