AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1330460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330460 MtrunA17_Chr4g0063401 94.355 248 13 1 1 247 1 248 1.51e-177 491
Msa1330460 MtrunA17_Chr4g0063361 64.567 254 69 5 1 246 1 241 2.52e-116 336
Msa1330460 MtrunA17_Chr4g0063391 56.400 250 67 7 1 248 1 210 2.98e-86 259
Msa1330460 MtrunA17_Chr4g0063371 46.828 331 69 9 1 325 1 230 3.61e-74 228
Msa1330460 MtrunA17_Chr3g0098251 57.143 203 55 5 44 246 7 177 8.35e-73 224
Msa1330460 MtrunA17_Chr8g0360671 54.140 157 42 4 99 255 41 167 1.77e-46 155
Msa1330460 MtrunA17_Chr8g0366501 33.775 302 141 10 71 326 86 374 3.66e-40 144
Msa1330460 MtrunA17_Chr4g0063411 89.024 82 9 0 245 326 9 90 2.20e-33 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330460 AT3G19360.1 44.753 324 131 10 42 326 59 373 5.54e-76 238
Msa1330460 AT3G19360.2 44.753 324 131 10 42 326 59 373 5.54e-76 238
Msa1330460 AT1G32360.1 32.215 298 160 8 71 326 85 382 1.12e-35 133
Msa1330460 AT2G35430.1 30.392 204 108 8 138 326 66 250 8.14e-17 79.3
Msa1330460 AT2G35430.1 35.849 106 46 4 77 168 71 168 4.45e-11 62.8

Find 101 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTTCTCCAAAGTCTTT+TGG 0.221074 8_4:+49634123 Msa1330460:CDS
GCAATAAGCATTGGAAATAT+TGG 0.228785 8_4:+49634105 Msa1330460:CDS
ATGAGGATCCTGCTAGGTTT+AGG 0.244285 8_4:+49634052 Msa1330460:CDS
TGGGGATGGTTCTAACAATT+TGG 0.253072 8_4:+49634143 Msa1330460:CDS
CTAACAATTTGGAAGGCATT+AGG 0.254194 8_4:+49634154 Msa1330460:CDS
AAGAAGATCAATGGTATTTA+TGG 0.269407 8_4:+49636362 Msa1330460:CDS
CACTTACTTAGGTTGAAATT+AGG 0.275359 8_4:+49636338 Msa1330460:CDS
CAAGGCAGCCACCACCAAAT+TGG 0.283685 8_4:+49633935 Msa1330460:CDS
TGAGGATCCTGCTAGGTTTA+GGG 0.310738 8_4:+49634053 Msa1330460:CDS
CTAGGTTTAGGGATGATTCT+TGG 0.317545 8_4:+49634064 Msa1330460:CDS
GCAACACCTGTTGAGGCTTT+TGG 0.324209 8_4:-49636245 None:intergenic
TTCCAATAAGCGCTCCTTGC+TGG 0.328577 8_4:-49634189 None:intergenic
AGCCAGCAAGGAGCGCTTAT+TGG 0.331978 8_4:+49634187 Msa1330460:CDS
TCAATGGTATTTATGGTGAT+TGG 0.333200 8_4:+49636369 Msa1330460:CDS
CACTTCAATGATATCGTTGT+TGG 0.339985 8_4:-49633608 None:intergenic
ATACAGGGAGCTGTCCCTTT+GGG 0.341839 8_4:+49634235 Msa1330460:CDS
ACCTGAACTTAGTGCAGATT+CGG 0.365276 8_4:-49633789 None:intergenic
TCAAAGTGATCGTTATTGAT+TGG 0.371371 8_4:-49633643 None:intergenic
TAACAATTTGGAAGGCATTA+GGG 0.381170 8_4:+49634155 Msa1330460:CDS
CAAAGTGATCGTTATTGATT+GGG 0.381558 8_4:-49633642 None:intergenic
TAAGGCAGCCACCGCGGAAT+TGG 0.383871 8_4:+49633866 Msa1330460:CDS
ATGCTGCTTGTTCCTCGGTT+TGG 0.390724 8_4:-49633754 None:intergenic
AACGGTGAGGAATGTCCTTA+TGG 0.402090 8_4:+49634009 Msa1330460:CDS
AGGTTTGGTACTTGTAGAAA+TGG 0.405516 8_4:+49633808 Msa1330460:CDS
CCCGAATCTGCACTAAGTTC+AGG 0.412306 8_4:+49633788 Msa1330460:CDS
ATCTGCACTAAGTTCAGGTT+TGG 0.426557 8_4:+49633793 Msa1330460:CDS
CCTGAACTTAGTGCAGATTC+GGG 0.429469 8_4:-49633788 None:intergenic
CATACAGGGAGCTGTCCCTT+TGG 0.435906 8_4:+49634234 Msa1330460:CDS
GATTGATATGCCCTCCTTCA+AGG 0.436742 8_4:+49633716 Msa1330460:CDS
CTGCTTGTTCCTCGGTTTGG+TGG 0.444819 8_4:-49633751 None:intergenic
GCAGCCACCGCGGAATTGGC+AGG 0.444919 8_4:+49633870 Msa1330460:CDS
AAGGCAGCCACCACCAAATT+GGG 0.448398 8_4:+49633936 Msa1330460:CDS
CTGTGTCAATGGCAGCATAC+AGG 0.450029 8_4:+49634219 Msa1330460:CDS
GAACCATCCCCAAAAGACTT+TGG 0.450065 8_4:-49634132 None:intergenic
CTTGTTCCTCGGTTTGGTGG+AGG 0.456539 8_4:-49633748 None:intergenic
CACTTTGCTCATGGAGAAGC+AGG 0.457533 8_4:+49634267 Msa1330460:CDS
ATAAGAAGAAGCACTTACTT+AGG 0.466516 8_4:+49636327 Msa1330460:CDS
AGCAATTCTCGCCCTCTTGA+AGG 0.471550 8_4:-49633674 None:intergenic
AATATCGTCTGGAAAATTCA+TGG 0.472650 8_4:-49633548 None:intergenic
CTACAGGTTGGAATATCGTC+TGG 0.476700 8_4:-49633559 None:intergenic
AAAATTGCAATTACGCTCAC+GGG 0.487194 8_4:+49633833 Msa1330460:CDS
ACTTCACCAAAAGCCTCAAC+AGG 0.492958 8_4:+49636239 Msa1330460:CDS
TCGTTATTGATTGGGATTTG+AGG 0.495130 8_4:-49633634 None:intergenic
GGAGGCTGAATCATCCTTGA+AGG 0.501461 8_4:-49633730 None:intergenic
GAGACCTGTCACTTTGCTCA+TGG 0.502581 8_4:+49634258 Msa1330460:CDS
CTCACGGGGCTGATGAGATA+AGG 0.504892 8_4:+49633848 Msa1330460:CDS
TTGATCCTCGCAACGACGAA+CGG 0.512205 8_4:+49633899 Msa1330460:CDS
CATGACAAAGGAAGAGGAGC+AGG 0.513127 8_4:+49636304 Msa1330460:CDS
TGATCTTCATCCCAATTTGG+TGG 0.514081 8_4:-49633946 None:intergenic
GAGTTACAAGCTCCTGGTGG+AGG 0.514447 8_4:+49636161 Msa1330460:intron
AAGCTACACTTATCACCATA+AGG 0.515098 8_4:-49634024 None:intergenic
GAAGGTAGGAGTAGAATCAT+TGG 0.517961 8_4:-49636267 None:intergenic
CTTTGTCATGAGTAGACTTG+TGG 0.519083 8_4:-49636291 None:intergenic
TTCTCCATGAGCAAAGTGAC+AGG 0.523166 8_4:-49634262 None:intergenic
TTCTTCATGAGGATCCTGCT+AGG 0.523992 8_4:+49634046 Msa1330460:CDS
GGAAGACAAAGCTGTGTCAA+TGG 0.532349 8_4:+49634208 Msa1330460:CDS
ATCATTGGCAACACCTGTTG+AGG 0.534873 8_4:-49636252 None:intergenic
GAATCATCCCTAAACCTAGC+AGG 0.537317 8_4:-49634060 None:intergenic
TCTTCATCCCAATTTGGTGG+TGG 0.537541 8_4:-49633943 None:intergenic
GATGGTTCTAACAATTTGGA+AGG 0.539743 8_4:+49634147 Msa1330460:CDS
GAGTGTTCTGCAATAAGCAT+TGG 0.542316 8_4:+49634096 Msa1330460:CDS
AAGTGACAGGTCTCCCCAAA+GGG 0.549845 8_4:-49634249 None:intergenic
TGTGTCAATGGCAGCATACA+GGG 0.552109 8_4:+49634220 Msa1330460:CDS
CTGCGGTGGTGACATCCTAC+AGG 0.555913 8_4:-49633575 None:intergenic
GAAAATTGCAATTACGCTCA+CGG 0.559384 8_4:+49633832 Msa1330460:CDS
AAATCAGAGTTACAAGCTCC+TGG 0.561857 8_4:+49636155 Msa1330460:intron
AAATTAGGCAAGAAGATCAA+TGG 0.564267 8_4:+49636353 Msa1330460:CDS
AGTAGACTTGTGGTGAAGGT+AGG 0.573300 8_4:-49636281 None:intergenic
CAACGACGAACGGCTTCAGA+TGG 0.574258 8_4:+49633909 Msa1330460:CDS
TCAGCTTCAATGCCTCCACC+AGG 0.581762 8_4:-49636173 None:intergenic
GGCTGAATCATCCTTGAAGG+AGG 0.587591 8_4:-49633727 None:intergenic
TCTACTCATGACAAAGGAAG+AGG 0.589668 8_4:+49636298 Msa1330460:CDS
CTCGCCCTCTTGAAGGAATG+TGG 0.590307 8_4:-49633667 None:intergenic
TTGAGCCACATTCCTTCAAG+AGG 0.596203 8_4:+49633662 Msa1330460:CDS
TACAGGGAGCTGTCCCTTTG+GGG 0.596640 8_4:+49634236 Msa1330460:CDS
CATGAGTAGACTTGTGGTGA+AGG 0.601665 8_4:-49636285 None:intergenic
GGAGGGCATATCAATCCACT+AGG 0.602999 8_4:-49633709 None:intergenic
GGGCTGTGAACAAGCCAGCA+AGG 0.614710 8_4:+49634175 Msa1330460:CDS
TGAGCCACATTCCTTCAAGA+GGG 0.615845 8_4:+49633663 Msa1330460:CDS
TAAGTGTAGCTTTCTTCATG+AGG 0.621800 8_4:+49634035 Msa1330460:CDS
TGAAGCCGTTCGTCGTTGCG+AGG 0.623112 8_4:-49633904 None:intergenic
AAAGTGACAGGTCTCCCCAA+AGG 0.626382 8_4:-49634250 None:intergenic
CAGACGATATTCCAACCTGT+AGG 0.630277 8_4:+49633560 Msa1330460:CDS
AGCTCCTGCCAATTCCGCGG+TGG 0.631980 8_4:-49633874 None:intergenic
CACAAGTCTACTCATGACAA+AGG 0.632619 8_4:+49636292 Msa1330460:CDS
GAGGGCATATCAATCCACTA+GGG 0.647490 8_4:-49633708 None:intergenic
AACGGCTTCAGATGGAGACA+AGG 0.650858 8_4:+49633917 Msa1330460:CDS
GCTGAATCATCCTTGAAGGA+GGG 0.652254 8_4:-49633726 None:intergenic
ACTAGCTCCTGCCAATTCCG+CGG 0.663260 8_4:-49633877 None:intergenic
GGTGGTGACATCCTACAGGT+TGG 0.681473 8_4:-49633571 None:intergenic
ATGAGATAAGGCAGCCACCG+CGG 0.683865 8_4:+49633860 Msa1330460:CDS
TCAGAGTTACAAGCTCCTGG+TGG 0.696701 8_4:+49636158 Msa1330460:intron
GGGCATATCAATCCACTAGG+GGG 0.701239 8_4:-49633706 None:intergenic
ACAACGATATCATTGAAGTG+AGG 0.706867 8_4:+49633611 Msa1330460:CDS
ATTCAGCCTCCACCAAACCG+AGG 0.710090 8_4:+49633742 Msa1330460:CDS
AAATTGCAATTACGCTCACG+GGG 0.722741 8_4:+49633834 Msa1330460:CDS
CTGTGCATGAAGTATTACAA+CGG 0.723396 8_4:+49633991 Msa1330460:CDS
CATGAAGTATTACAACGGTG+AGG 0.726258 8_4:+49633996 Msa1330460:CDS
AGGGCATATCAATCCACTAG+GGG 0.727577 8_4:-49633707 None:intergenic
TTGGATGAAGCAAACTGCGG+TGG 0.740311 8_4:-49633589 None:intergenic
TTGTTGGATGAAGCAAACTG+CGG 0.811729 8_4:-49633592 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTTTTTTGCATATT+TGG + chr8_4:49636034-49636053 Msa1330460:intron 10.0%
!! GTGATTGAATATTTAAATTT+AGG - chr8_4:49635080-49635099 None:intergenic 15.0%
!!! TTCTAATTCAAAAGTAATTT+TGG - chr8_4:49635308-49635327 None:intergenic 15.0%
!! CAAAACCATTAATCAAAATA+GGG - chr8_4:49634328-49634347 None:intergenic 20.0%
!! TCAAAACCATTAATCAAAAT+AGG - chr8_4:49634329-49634348 None:intergenic 20.0%
!! AAATAAAAATTCCTGACAAT+GGG - chr8_4:49635037-49635056 None:intergenic 20.0%
!! AATCATCCATAATAATATGA+AGG - chr8_4:49635102-49635121 None:intergenic 20.0%
!!! TTATATGTCATGCTTATTTT+TGG + chr8_4:49635507-49635526 Msa1330460:intron 20.0%
!! AATGTTTGACTGTAAAATTA+AGG - chr8_4:49635896-49635915 None:intergenic 20.0%
!! ACAATTACAATAGATAAGAA+AGG + chr8_4:49636061-49636080 Msa1330460:intron 20.0%
! ATTAATCAAAATAGGGAGAA+TGG - chr8_4:49634321-49634340 None:intergenic 25.0%
!!! ATTCTCCCTATTTTGATTAA+TGG + chr8_4:49634320-49634339 Msa1330460:intron 25.0%
!!! TTCTTTCAGTTTTGAAGTAA+TGG + chr8_4:49634586-49634605 Msa1330460:intron 25.0%
! AAAAATCTGGTATAGAAACA+TGG - chr8_4:49634739-49634758 None:intergenic 25.0%
!!! ATGTTTCTATACCAGATTTT+TGG + chr8_4:49634738-49634757 Msa1330460:intron 25.0%
! AATAAAAATTCCTGACAATG+GGG - chr8_4:49635036-49635055 None:intergenic 25.0%
! CAAATAAAAATTCCTGACAA+TGG - chr8_4:49635038-49635057 None:intergenic 25.0%
! CAATCACCTTCATATTATTA+TGG + chr8_4:49635093-49635112 Msa1330460:intron 25.0%
!!! AAGTAATTTTGGTACATACT+TGG - chr8_4:49635297-49635316 None:intergenic 25.0%
! TTATAACAATTGAAGCTGTT+TGG + chr8_4:49636087-49636106 Msa1330460:intron 25.0%
! AAGAAGATCAATGGTATTTA+TGG + chr8_4:49636362-49636381 Msa1330460:CDS 25.0%
! CAAAGTGATCGTTATTGATT+GGG - chr8_4:49633645-49633664 None:intergenic 30.0%
TCAAAGTGATCGTTATTGAT+TGG - chr8_4:49633646-49633665 None:intergenic 30.0%
! TTTTGATCTTCATCCCAATT+TGG - chr8_4:49633952-49633971 None:intergenic 30.0%
!! GCAATAAGCATTGGAAATAT+TGG + chr8_4:49634105-49634124 Msa1330460:CDS 30.0%
!! TAACAATTTGGAAGGCATTA+GGG + chr8_4:49634155-49634174 Msa1330460:CDS 30.0%
TTCTATGATGATTGTGTAGA+AGG + chr8_4:49634393-49634412 Msa1330460:intron 30.0%
TGTTGTGTATGATATTTCCA+TGG + chr8_4:49634474-49634493 Msa1330460:intron 30.0%
ATATCATACACAACAACGAT+AGG - chr8_4:49634567-49634586 None:intergenic 30.0%
TCTTAGTGTTGTCAAATTCA+TGG + chr8_4:49634641-49634660 Msa1330460:intron 30.0%
! TATCCTAAAAAGCATATCAC+TGG + chr8_4:49634906-49634925 Msa1330460:intron 30.0%
TTGTTAAATAGCGACTATAG+CGG - chr8_4:49634970-49634989 None:intergenic 30.0%
! TATGGATGATTGCTATTGTA+TGG + chr8_4:49635111-49635130 Msa1330460:intron 30.0%
AATCAACAAATGTAAAGCTG+TGG - chr8_4:49635159-49635178 None:intergenic 30.0%
! CTTTACATTTGTTGATTGAC+TGG + chr8_4:49635162-49635181 Msa1330460:intron 30.0%
!! GATACACTAGGATAGTTTTA+CGG + chr8_4:49635347-49635366 Msa1330460:intron 30.0%
ATGATACCAAGTACGAATAT+CGG - chr8_4:49635604-49635623 None:intergenic 30.0%
AAAGATAAAAGCAGTCACTT+CGG - chr8_4:49635668-49635687 None:intergenic 30.0%
TGTCCGACTCATAAAAAAAT+TGG + chr8_4:49635822-49635841 Msa1330460:intron 30.0%
!!! CCTCCAATTTTTTTATGAGT+CGG - chr8_4:49635828-49635847 None:intergenic 30.0%
AATATAAAACCTCGCAAATG+AGG - chr8_4:49635857-49635876 None:intergenic 30.0%
! TCAAACATTTTTAATGCAGC+TGG + chr8_4:49635907-49635926 Msa1330460:intron 30.0%
!! ATAAGAAGAAGCACTTACTT+AGG + chr8_4:49636327-49636346 Msa1330460:CDS 30.0%
!! CACTTACTTAGGTTGAAATT+AGG + chr8_4:49636338-49636357 Msa1330460:CDS 30.0%
AAATTAGGCAAGAAGATCAA+TGG + chr8_4:49636353-49636372 Msa1330460:CDS 30.0%
TCAATGGTATTTATGGTGAT+TGG + chr8_4:49636369-49636388 Msa1330460:CDS 30.0%
CACTTCAATGATATCGTTGT+TGG - chr8_4:49633611-49633630 None:intergenic 35.0%
ACAACGATATCATTGAAGTG+AGG + chr8_4:49633611-49633630 Msa1330460:CDS 35.0%
! TCGTTATTGATTGGGATTTG+AGG - chr8_4:49633637-49633656 None:intergenic 35.0%
! AAAAAATGCTGCTTGTTCCT+CGG - chr8_4:49633762-49633781 None:intergenic 35.0%
! AGGTTTGGTACTTGTAGAAA+TGG + chr8_4:49633808-49633827 Msa1330460:CDS 35.0%
GAAAATTGCAATTACGCTCA+CGG + chr8_4:49633832-49633851 Msa1330460:CDS 35.0%
AAAATTGCAATTACGCTCAC+GGG + chr8_4:49633833-49633852 Msa1330460:CDS 35.0%
! CTGTGCATGAAGTATTACAA+CGG + chr8_4:49633991-49634010 Msa1330460:CDS 35.0%
AAGCTACACTTATCACCATA+AGG - chr8_4:49634027-49634046 None:intergenic 35.0%
TAAGTGTAGCTTTCTTCATG+AGG + chr8_4:49634035-49634054 Msa1330460:CDS 35.0%
!! ATTGGTTCTCCAAAGTCTTT+TGG + chr8_4:49634123-49634142 Msa1330460:CDS 35.0%
!! TTGGTTCTCCAAAGTCTTTT+GGG + chr8_4:49634124-49634143 Msa1330460:CDS 35.0%
!! GATGGTTCTAACAATTTGGA+AGG + chr8_4:49634147-49634166 Msa1330460:CDS 35.0%
!! CTAACAATTTGGAAGGCATT+AGG + chr8_4:49634154-49634173 Msa1330460:CDS 35.0%
ATCATACACAACAACGAAAG+TGG - chr8_4:49634467-49634486 None:intergenic 35.0%
GTGTATGATATTTCCATGGT+TGG + chr8_4:49634478-49634497 Msa1330460:intron 35.0%
AGTTTATGTCAAACCAACCA+TGG - chr8_4:49634494-49634513 None:intergenic 35.0%
! GGAAAACTTGTTTGTACGAT+CGG + chr8_4:49634607-49634626 Msa1330460:intron 35.0%
TATGGTAGCTGGAACATAAT+CGG - chr8_4:49634667-49634686 None:intergenic 35.0%
! TTATGATAATGCTGCCCTAT+TGG + chr8_4:49634786-49634805 Msa1330460:intron 35.0%
ATACACAGTGTTGTCAATTG+TGG + chr8_4:49634813-49634832 Msa1330460:intron 35.0%
AGCTATTACACTGTTGCATA+GGG - chr8_4:49634869-49634888 None:intergenic 35.0%
!! TGTCCAGTGATATGCTTTTT+AGG - chr8_4:49634912-49634931 None:intergenic 35.0%
TGTTAAATAGCGACTATAGC+GGG - chr8_4:49634969-49634988 None:intergenic 35.0%
! GACTGGAACTTTTAGATGTT+TGG + chr8_4:49635179-49635198 Msa1330460:intron 35.0%
TTGTATCCGATATTCGTACT+TGG + chr8_4:49635595-49635614 Msa1330460:intron 35.0%
TAACCTTATCCATGCAACAT+AGG + chr8_4:49635625-49635644 Msa1330460:intron 35.0%
GAGTCGGACATTAAATTTAC+AGG - chr8_4:49635812-49635831 None:intergenic 35.0%
CCGACTCATAAAAAAATTGG+AGG + chr8_4:49635825-49635844 Msa1330460:intron 35.0%
TGTCAAAAACCTTAACGTCT+TGG + chr8_4:49635954-49635973 Msa1330460:intron 35.0%
! GGTTTCCATGTATTTTGTTC+TGG + chr8_4:49636108-49636127 Msa1330460:intron 35.0%
ATCAGCCAGAACAAAATACA+TGG - chr8_4:49636116-49636135 None:intergenic 35.0%
! AGCTTTTGTTGAGCTTACTA+TGG - chr8_4:49636210-49636229 None:intergenic 35.0%
TTGTTGGATGAAGCAAACTG+CGG - chr8_4:49633595-49633614 None:intergenic 40.0%
ACCTGAACTTAGTGCAGATT+CGG - chr8_4:49633792-49633811 None:intergenic 40.0%
! ATCTGCACTAAGTTCAGGTT+TGG + chr8_4:49633793-49633812 Msa1330460:CDS 40.0%
AAATTGCAATTACGCTCACG+GGG + chr8_4:49633834-49633853 Msa1330460:CDS 40.0%
TGATCTTCATCCCAATTTGG+TGG - chr8_4:49633949-49633968 None:intergenic 40.0%
! CATGAAGTATTACAACGGTG+AGG + chr8_4:49633996-49634015 Msa1330460:CDS 40.0%
CTAGGTTTAGGGATGATTCT+TGG + chr8_4:49634064-49634083 Msa1330460:CDS 40.0%
GAGTGTTCTGCAATAAGCAT+TGG + chr8_4:49634096-49634115 Msa1330460:CDS 40.0%
! TGGTTCTCCAAAGTCTTTTG+GGG + chr8_4:49634125-49634144 Msa1330460:CDS 40.0%
!! TGGGGATGGTTCTAACAATT+TGG + chr8_4:49634143-49634162 Msa1330460:CDS 40.0%
CATACACAACAACGATAGGA+AGG - chr8_4:49634563-49634582 None:intergenic 40.0%
GATTATGTTCCAGCTACCAT+AGG + chr8_4:49634666-49634685 Msa1330460:intron 40.0%
ATAATAGCCATTGCAGCCTA+TGG - chr8_4:49634685-49634704 None:intergenic 40.0%
GCTGCAATGGCTATTATATC+TGG + chr8_4:49634688-49634707 Msa1330460:intron 40.0%
GCATTATCATAAGTGGAAGG+TGG - chr8_4:49634778-49634797 None:intergenic 40.0%
GCAGCATTATCATAAGTGGA+AGG - chr8_4:49634781-49634800 None:intergenic 40.0%
TAGGGCAGCATTATCATAAG+TGG - chr8_4:49634785-49634804 None:intergenic 40.0%
CACTGTGTATACCTCCAATA+GGG - chr8_4:49634803-49634822 None:intergenic 40.0%
ACACTGTGTATACCTCCAAT+AGG - chr8_4:49634804-49634823 None:intergenic 40.0%
CAGCTATTACACTGTTGCAT+AGG - chr8_4:49634870-49634889 None:intergenic 40.0%
GTTAAATAGCGACTATAGCG+GGG - chr8_4:49634968-49634987 None:intergenic 40.0%
CTGCATAGTCGATCAAAAGA+AGG - chr8_4:49635224-49635243 None:intergenic 40.0%
! TTTTGATCGACTATGCAGCA+TGG + chr8_4:49635226-49635245 Msa1330460:intron 40.0%
TCACACTCTTACTGTACTTG+TGG + chr8_4:49635264-49635283 Msa1330460:intron 40.0%
ATACTCCACTTGGATACACT+AGG + chr8_4:49635335-49635354 Msa1330460:intron 40.0%
ACTATCCTAGTGTATCCAAG+TGG - chr8_4:49635343-49635362 None:intergenic 40.0%
ACACCTATGTTGCATGGATA+AGG - chr8_4:49635631-49635650 None:intergenic 40.0%
TATCCATGCAACATAGGTGT+CGG + chr8_4:49635631-49635650 Msa1330460:intron 40.0%
! TCCAATCCTGTTTCGTCTTT+TGG + chr8_4:49635770-49635789 Msa1330460:intron 40.0%
ACCAAAAGACGAAACAGGAT+TGG - chr8_4:49635774-49635793 None:intergenic 40.0%
CAGAAACCAAAAGACGAAAC+AGG - chr8_4:49635779-49635798 None:intergenic 40.0%
!!! CGTCTTTTGGTTTCTGCTAA+TGG + chr8_4:49635783-49635802 Msa1330460:intron 40.0%
AAGGTATGCGACAAAAACTG+TGG - chr8_4:49635980-49635999 None:intergenic 40.0%
! TGTTCTGGCTGATGAAGTAT+TGG + chr8_4:49636123-49636142 Msa1330460:intron 40.0%
AAATCAGAGTTACAAGCTCC+TGG + chr8_4:49636155-49636174 Msa1330460:intron 40.0%
GAAGGTAGGAGTAGAATCAT+TGG - chr8_4:49636270-49636289 None:intergenic 40.0%
CTTTGTCATGAGTAGACTTG+TGG - chr8_4:49636294-49636313 None:intergenic 40.0%
CACAAGTCTACTCATGACAA+AGG + chr8_4:49636292-49636311 Msa1330460:CDS 40.0%
TCTACTCATGACAAAGGAAG+AGG + chr8_4:49636298-49636317 Msa1330460:CDS 40.0%
! CTACAGGTTGGAATATCGTC+TGG - chr8_4:49633562-49633581 None:intergenic 45.0%
CAGACGATATTCCAACCTGT+AGG + chr8_4:49633560-49633579 Msa1330460:CDS 45.0%
TTGAGCCACATTCCTTCAAG+AGG + chr8_4:49633662-49633681 Msa1330460:CDS 45.0%
TGAGCCACATTCCTTCAAGA+GGG + chr8_4:49633663-49633682 Msa1330460:CDS 45.0%
AGGGCATATCAATCCACTAG+GGG - chr8_4:49633710-49633729 None:intergenic 45.0%
! GAGGGCATATCAATCCACTA+GGG - chr8_4:49633711-49633730 None:intergenic 45.0%
GATTGATATGCCCTCCTTCA+AGG + chr8_4:49633716-49633735 Msa1330460:CDS 45.0%
GCTGAATCATCCTTGAAGGA+GGG - chr8_4:49633729-49633748 None:intergenic 45.0%
CCTGAACTTAGTGCAGATTC+GGG - chr8_4:49633791-49633810 None:intergenic 45.0%
! TCTTCATCCCAATTTGGTGG+TGG - chr8_4:49633946-49633965 None:intergenic 45.0%
AACGGTGAGGAATGTCCTTA+TGG + chr8_4:49634009-49634028 Msa1330460:CDS 45.0%
TTCTTCATGAGGATCCTGCT+AGG + chr8_4:49634046-49634065 Msa1330460:CDS 45.0%
ATGAGGATCCTGCTAGGTTT+AGG + chr8_4:49634052-49634071 Msa1330460:CDS 45.0%
TGAGGATCCTGCTAGGTTTA+GGG + chr8_4:49634053-49634072 Msa1330460:CDS 45.0%
GAATCATCCCTAAACCTAGC+AGG - chr8_4:49634063-49634082 None:intergenic 45.0%
!! TCTCCAAAGTCTTTTGGGGA+TGG + chr8_4:49634129-49634148 Msa1330460:CDS 45.0%
GAACCATCCCCAAAAGACTT+TGG - chr8_4:49634135-49634154 None:intergenic 45.0%
GGAAGACAAAGCTGTGTCAA+TGG + chr8_4:49634208-49634227 Msa1330460:CDS 45.0%
TGTGTCAATGGCAGCATACA+GGG + chr8_4:49634220-49634239 Msa1330460:CDS 45.0%
TTCTCCATGAGCAAAGTGAC+AGG - chr8_4:49634265-49634284 None:intergenic 45.0%
TTCTGCGAAGACAAGGCAAA+GGG - chr8_4:49634536-49634555 None:intergenic 45.0%
TTTCTGCGAAGACAAGGCAA+AGG - chr8_4:49634537-49634556 None:intergenic 45.0%
!! ATCTGGCTTGTTTCCACCAT+AGG + chr8_4:49634705-49634724 Msa1330460:intron 45.0%
AAACATGGCATTGCAGCCTA+TGG - chr8_4:49634724-49634743 None:intergenic 45.0%
ACAGCAGCTAGCCAAAAATC+TGG - chr8_4:49634752-49634771 None:intergenic 45.0%
! TGATAATGCTGCCCTATTGG+AGG + chr8_4:49634789-49634808 Msa1330460:intron 45.0%
! TTCCTGACAATGGGGTTGAT+GGG - chr8_4:49635028-49635047 None:intergenic 45.0%
ATTCCTGACAATGGGGTTGA+TGG - chr8_4:49635029-49635048 None:intergenic 45.0%
GAAACGCTAGATACTCCACT+TGG + chr8_4:49635325-49635344 Msa1330460:intron 45.0%
TTACCGACACCTATGTTGCA+TGG - chr8_4:49635637-49635656 None:intergenic 45.0%
! AAAAGCAGTCACTTCGGTGT+GGG - chr8_4:49635662-49635681 None:intergenic 45.0%
TAAAAGCAGTCACTTCGGTG+TGG - chr8_4:49635663-49635682 None:intergenic 45.0%
AGGTACATTCCTCATTTGCG+AGG + chr8_4:49635845-49635864 Msa1330460:intron 45.0%
AAACTGTGGCCAAGACGTTA+AGG - chr8_4:49635966-49635985 None:intergenic 45.0%
! GTTGAGGCTTTTGGTGAAGT+TGG - chr8_4:49636239-49636258 None:intergenic 45.0%
ACTTCACCAAAAGCCTCAAC+AGG + chr8_4:49636239-49636258 Msa1330460:CDS 45.0%
ATCATTGGCAACACCTGTTG+AGG - chr8_4:49636255-49636274 None:intergenic 45.0%
AGTAGACTTGTGGTGAAGGT+AGG - chr8_4:49636284-49636303 None:intergenic 45.0%
CATGAGTAGACTTGTGGTGA+AGG - chr8_4:49636288-49636307 None:intergenic 45.0%
!!! GATTTTATTTTTTTAAATAA+AGG - chr8_4:49635999-49636018 None:intergenic 5.0%
TTGGATGAAGCAAACTGCGG+TGG - chr8_4:49633592-49633611 None:intergenic 50.0%
AGCAATTCTCGCCCTCTTGA+AGG - chr8_4:49633677-49633696 None:intergenic 50.0%
GCTGATAACAATCCCCCTAG+TGG + chr8_4:49633694-49633713 Msa1330460:CDS 50.0%
GGGCATATCAATCCACTAGG+GGG - chr8_4:49633709-49633728 None:intergenic 50.0%
! GGAGGGCATATCAATCCACT+AGG - chr8_4:49633712-49633731 None:intergenic 50.0%
GGCTGAATCATCCTTGAAGG+AGG - chr8_4:49633730-49633749 None:intergenic 50.0%
GGAGGCTGAATCATCCTTGA+AGG - chr8_4:49633733-49633752 None:intergenic 50.0%
! ATGCTGCTTGTTCCTCGGTT+TGG - chr8_4:49633757-49633776 None:intergenic 50.0%
CCCGAATCTGCACTAAGTTC+AGG + chr8_4:49633788-49633807 Msa1330460:CDS 50.0%
TTGATCCTCGCAACGACGAA+CGG + chr8_4:49633899-49633918 Msa1330460:CDS 50.0%
AACGGCTTCAGATGGAGACA+AGG + chr8_4:49633917-49633936 Msa1330460:CDS 50.0%
AAGGCAGCCACCACCAAATT+GGG + chr8_4:49633936-49633955 Msa1330460:CDS 50.0%
TTCCAATAAGCGCTCCTTGC+TGG - chr8_4:49634192-49634211 None:intergenic 50.0%
CTGTGTCAATGGCAGCATAC+AGG + chr8_4:49634219-49634238 Msa1330460:CDS 50.0%
ATACAGGGAGCTGTCCCTTT+GGG + chr8_4:49634235-49634254 Msa1330460:CDS 50.0%
AAGTGACAGGTCTCCCCAAA+GGG - chr8_4:49634252-49634271 None:intergenic 50.0%
AAAGTGACAGGTCTCCCCAA+AGG - chr8_4:49634253-49634272 None:intergenic 50.0%
!! GAGACCTGTCACTTTGCTCA+TGG + chr8_4:49634258-49634277 Msa1330460:CDS 50.0%
CACTTTGCTCATGGAGAAGC+AGG + chr8_4:49634267-49634286 Msa1330460:CDS 50.0%
CAAAGGGGCAACAAACAGGA+GGG - chr8_4:49634520-49634539 None:intergenic 50.0%
AAGGCAAAGGGGCAACAAAC+AGG - chr8_4:49634524-49634543 None:intergenic 50.0%
TCTGCGAAGACAAGGCAAAG+GGG - chr8_4:49634535-49634554 None:intergenic 50.0%
AGGCACTTTCTGCGAAGACA+AGG - chr8_4:49634543-49634562 None:intergenic 50.0%
! ATAGCGGGGCTACTGCATAT+AGG - chr8_4:49634954-49634973 None:intergenic 50.0%
TTCCCATCAACCCCATTGTC+AGG + chr8_4:49635023-49635042 Msa1330460:intron 50.0%
TCAGAGTTACAAGCTCCTGG+TGG + chr8_4:49636158-49636177 Msa1330460:intron 50.0%
! GCAACACCTGTTGAGGCTTT+TGG - chr8_4:49636248-49636267 None:intergenic 50.0%
CATGACAAAGGAAGAGGAGC+AGG + chr8_4:49636304-49636323 Msa1330460:CDS 50.0%
GGTGGTGACATCCTACAGGT+TGG - chr8_4:49633574-49633593 None:intergenic 55.0%
CTCGCCCTCTTGAAGGAATG+TGG - chr8_4:49633670-49633689 None:intergenic 55.0%
ATTCAGCCTCCACCAAACCG+AGG + chr8_4:49633742-49633761 Msa1330460:CDS 55.0%
!! CTTGTTCCTCGGTTTGGTGG+AGG - chr8_4:49633751-49633770 None:intergenic 55.0%
!! CTGCTTGTTCCTCGGTTTGG+TGG - chr8_4:49633754-49633773 None:intergenic 55.0%
CTCACGGGGCTGATGAGATA+AGG + chr8_4:49633848-49633867 Msa1330460:CDS 55.0%
ATGAGATAAGGCAGCCACCG+CGG + chr8_4:49633860-49633879 Msa1330460:CDS 55.0%
ACTAGCTCCTGCCAATTCCG+CGG - chr8_4:49633880-49633899 None:intergenic 55.0%
CAACGACGAACGGCTTCAGA+TGG + chr8_4:49633909-49633928 Msa1330460:CDS 55.0%
CAAGGCAGCCACCACCAAAT+TGG + chr8_4:49633935-49633954 Msa1330460:CDS 55.0%
!! AGCCAGCAAGGAGCGCTTAT+TGG + chr8_4:49634187-49634206 Msa1330460:CDS 55.0%
CATACAGGGAGCTGTCCCTT+TGG + chr8_4:49634234-49634253 Msa1330460:CDS 55.0%
TACAGGGAGCTGTCCCTTTG+GGG + chr8_4:49634236-49634255 Msa1330460:CDS 55.0%
GCAAAGGGGCAACAAACAGG+AGG - chr8_4:49634521-49634540 None:intergenic 55.0%
CCATTGCAGCCTATGGTAGC+TGG - chr8_4:49634678-49634697 None:intergenic 55.0%
CCAGCTACCATAGGCTGCAA+TGG + chr8_4:49634675-49634694 Msa1330460:intron 55.0%
CATGGCATTGCAGCCTATGG+TGG - chr8_4:49634721-49634740 None:intergenic 55.0%
GAGTTACAAGCTCCTGGTGG+AGG + chr8_4:49636161-49636180 Msa1330460:intron 55.0%
TCAGCTTCAATGCCTCCACC+AGG - chr8_4:49636176-49636195 None:intergenic 55.0%
CTGCGGTGGTGACATCCTAC+AGG - chr8_4:49633578-49633597 None:intergenic 60.0%
TAAGGCAGCCACCGCGGAAT+TGG + chr8_4:49633866-49633885 Msa1330460:CDS 60.0%
TGAAGCCGTTCGTCGTTGCG+AGG - chr8_4:49633907-49633926 None:intergenic 60.0%
GGGCTGTGAACAAGCCAGCA+AGG + chr8_4:49634175-49634194 Msa1330460:CDS 60.0%
AGCTCCTGCCAATTCCGCGG+TGG - chr8_4:49633877-49633896 None:intergenic 65.0%
GCAGCCACCGCGGAATTGGC+AGG + chr8_4:49633870-49633889 Msa1330460:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 49633550 49636403 49633550 ID=Msa1330460;Name=Msa1330460
chr8_4 mRNA 49633550 49636403 49633550 ID=Msa1330460-mRNA-1;Parent=Msa1330460;Name=Msa1330460-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|326
chr8_4 exon 49633550 49634288 49633550 ID=Msa1330460-mRNA-1:exon:14388;Parent=Msa1330460-mRNA-1
chr8_4 exon 49636162 49636403 49636162 ID=Msa1330460-mRNA-1:exon:14389;Parent=Msa1330460-mRNA-1
chr8_4 CDS 49633550 49634288 49633550 ID=Msa1330460-mRNA-1:cds;Parent=Msa1330460-mRNA-1
chr8_4 CDS 49636162 49636403 49636162 ID=Msa1330460-mRNA-1:cds;Parent=Msa1330460-mRNA-1
Gene Sequence

>Msa1330460

ATGAATTTTCCAGACGATATTCCAACCTGTAGGATGTCACCACCGCAGTTTGCTTCATCCAACAACGATATCATTGAAGTGAGGCCTCAAATCCCAATCAATAACGATCACTTTGAGCCACATTCCTTCAAGAGGGCGAGAATTGCTGATAACAATCCCCCTAGTGGATTGATATGCCCTCCTTCAAGGATGATTCAGCCTCCACCAAACCGAGGAACAAGCAGCATTTTTTTCAAGACCCGAATCTGCACTAAGTTCAGGTTTGGTACTTGTAGAAATGGCGAAAATTGCAATTACGCTCACGGGGCTGATGAGATAAGGCAGCCACCGCGGAATTGGCAGGAGCTAGTTGATCCTCGCAACGACGAACGGCTTCAGATGGAGACAAGGCAGCCACCACCAAATTGGGATGAAGATCAAAAAATCATTCACAAGATGAAGCTGTGCATGAAGTATTACAACGGTGAGGAATGTCCTTATGGTGATAAGTGTAGCTTTCTTCATGAGGATCCTGCTAGGTTTAGGGATGATTCTTGGAAAACAAGAGAGTGTTCTGCAATAAGCATTGGAAATATTGGTTCTCCAAAGTCTTTTGGGGATGGTTCTAACAATTTGGAAGGCATTAGGGCTGTGAACAAGCCAGCAAGGAGCGCTTATTGGAAGACAAAGCTGTGTCAATGGCAGCATACAGGGAGCTGTCCCTTTGGGGAGACCTGTCACTTTGCTCATGGAGAAGCAGAGTTACAAGCTCCTGGTGGAGGCATTGAAGCTGAAGCTGCAGTTGCCATAGTAAGCTCAACAAAAGCTATCATTCCAACTTCACCAAAAGCCTCAACAGGTGTTGCCAATGATTCTACTCCTACCTTCACCACAAGTCTACTCATGACAAAGGAAGAGGAGCAGGATAAGAAGAAGCACTTACTTAGGTTGAAATTAGGCAAGAAGATCAATGGTATTTATGGTGATTGGATTGATGATTGA
Protein sequence

>Msa1330460

MNFPDDIPTCRMSPPQFASSNNDIIEVRPQIPINNDHFEPHSFKRARIADNNPPSGLICPPSRMIQPPPNRGTSSIFFKTRICTKFRFGTCRNGENCNYAHGADEIRQPPRNWQELVDPRNDERLQMETRQPPPNWDEDQKIIHKMKLCMKYYNGEECPYGDKCSFLHEDPARFRDDSWKTRECSAISIGNIGSPKSFGDGSNNLEGIRAVNKPARSAYWKTKLCQWQHTGSCPFGETCHFAHGEAELQAPGGGIEAEAAVAIVSSTKAIIPTSPKASTGVANDSTPTFTTSLLMTKEEEQDKKKHLLRLKLGKKINGIYGDWIDD*