AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1334730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334730 MtrunA17_Chr4g0060841 90.730 356 7 1 1 330 1 356 0.0 655
Msa1334730 MtrunA17_Chr3g0134451 35.211 213 106 8 1 191 1 203 2.56e-26 106
Msa1334730 MtrunA17_Chr5g0417661 70.149 67 20 0 1 67 56 122 2.80e-25 98.6
Msa1334730 MtrunA17_Chr5g0417671 81.132 53 10 0 59 111 26 78 4.06e-23 91.7
Msa1334730 MtrunA17_Chr5g0425191 62.295 61 23 0 1 61 1 61 2.33e-21 87.4
Msa1334730 MtrunA17_Chr4g0044071 63.934 61 22 0 1 61 1 61 1.23e-20 85.9
Msa1334730 MtrunA17_Chr5g0427401 39.456 147 79 4 1 143 1 141 2.68e-18 82.4
Msa1334730 MtrunA17_Chr1g0159661 39.423 104 63 0 1 104 1 104 4.57e-17 77.8
Msa1334730 MtrunA17_Chr3g0125241 62.264 53 20 0 1 53 26 78 4.32e-16 75.9
Msa1334730 MtrunA17_Chr1g0173161 58.491 53 22 0 1 53 1 53 7.86e-16 71.6
Msa1334730 MtrunA17_Chr5g0412461 35.811 148 75 5 1 138 1 138 3.32e-15 73.9
Msa1334730 MtrunA17_Chr5g0412271 38.028 142 80 5 1 138 1 138 3.34e-15 73.9
Msa1334730 MtrunA17_Chr7g0246431 31.176 170 85 5 1 154 1 154 4.13e-15 73.2
Msa1334730 MtrunA17_Chr4g0020221 46.479 71 36 1 1 71 1 69 5.92e-15 73.6
Msa1334730 MtrunA17_Chr1g0164791 32.099 162 93 5 1 147 1 160 6.59e-15 73.2
Msa1334730 MtrunA17_Chr4g0057151 34.783 161 89 6 1 153 1 153 6.98e-15 72.4
Msa1334730 MtrunA17_Chr3g0142191 56.604 53 23 0 1 53 1 53 7.21e-15 72.8
Msa1334730 MtrunA17_Chr2g0308251 54.717 53 24 0 1 53 1 53 7.31e-15 69.3
Msa1334730 MtrunA17_Chr8g0351041 30.719 153 101 2 1 150 1 151 7.41e-15 71.2
Msa1334730 MtrunA17_Chr2g0279821 46.479 71 36 1 1 71 1 69 7.85e-15 73.2
Msa1334730 MtrunA17_Chr2g0284911 45.070 71 37 1 1 71 16 84 7.95e-15 73.2
Msa1334730 MtrunA17_Chr5g0427921 50.943 53 26 0 1 53 1 53 8.22e-15 68.2
Msa1334730 MtrunA17_Chr5g0420081 45.570 79 42 1 1 79 1 78 8.39e-15 72.8
Msa1334730 MtrunA17_Chr8g0366231 43.038 79 44 1 1 79 1 78 1.28e-14 72.4
Msa1334730 MtrunA17_Chr3g0097101 54.717 53 24 0 1 53 16 68 1.82e-14 72.0
Msa1334730 MtrunA17_Chr4g0061691 43.038 79 44 1 1 79 1 78 2.41e-14 71.6
Msa1334730 MtrunA17_Chr3g0077501 54.717 53 24 0 1 53 1 53 2.42e-14 71.2
Msa1334730 MtrunA17_Chr4g0049901 48.571 70 34 1 1 70 27 94 3.00e-14 71.2
Msa1334730 MtrunA17_Chr8g0380021 33.562 146 89 5 1 142 17 158 3.55e-14 71.2
Msa1334730 MtrunA17_Chr7g0246381 54.717 53 24 0 1 53 1 53 5.35e-14 66.2
Msa1334730 MtrunA17_Chr4g0049541 45.946 74 38 1 1 74 1 72 6.60e-14 70.1
Msa1334730 MtrunA17_Chr5g0411691 43.662 71 38 1 1 71 1 69 7.82e-14 70.1
Msa1334730 MtrunA17_Chr1g0204111 46.575 73 37 1 1 73 17 87 1.01e-13 70.1
Msa1334730 MtrunA17_Chr8g0351031 30.128 156 104 2 1 153 1 154 1.64e-13 68.9
Msa1334730 MtrunA17_Chr8g0377561 50.943 53 26 0 1 53 1 53 1.68e-13 65.9
Msa1334730 MtrunA17_Chr1g0175611 38.961 77 45 1 1 77 1 75 2.91e-13 67.0
Msa1334730 MtrunA17_Chr7g0220121 30.612 147 91 4 1 142 1 141 3.31e-13 68.6
Msa1334730 MtrunA17_Chr4g0061671 50.943 53 26 0 1 53 1 53 3.69e-13 67.0
Msa1334730 MtrunA17_Chr8g0351051 52.830 53 25 0 1 53 1 53 4.19e-13 68.2
Msa1334730 MtrunA17_Chr7g0220111 50.943 53 26 0 1 53 1 53 5.66e-13 67.8
Msa1334730 MtrunA17_Chr5g0420091 52.830 53 25 0 1 53 1 53 7.18e-13 67.0
Msa1334730 MtrunA17_Chr1g0208561 42.667 75 41 1 2 76 9 81 7.76e-13 67.0
Msa1334730 MtrunA17_Chr5g0404951 33.544 158 72 6 1 154 1 129 8.33e-13 67.0
Msa1334730 MtrunA17_Chr8g0379021 51.923 52 25 0 2 53 12 63 8.55e-13 65.9
Msa1334730 MtrunA17_Chr3g0089141 42.667 75 41 1 2 76 9 81 8.71e-13 67.0
Msa1334730 MtrunA17_Chr1g0166611 37.363 91 51 2 16 104 20 106 9.90e-13 64.7
Msa1334730 MtrunA17_Chr6g0455511 50.943 53 26 0 1 53 1 53 1.80e-12 66.2
Msa1334730 MtrunA17_Chr3g0122251 50.943 53 26 0 1 53 1 53 2.20e-12 66.2
Msa1334730 MtrunA17_Chr4g0052081 53.704 54 25 0 1 54 1 54 2.63e-12 64.7
Msa1334730 MtrunA17_Chr6g0474371 43.243 74 39 2 1 73 1 72 2.77e-12 64.7
Msa1334730 MtrunA17_Chr1g0208581 30.625 160 87 5 2 141 9 164 3.22e-12 65.5
Msa1334730 MtrunA17_Chr2g0333981 50.943 53 26 0 1 53 11 63 3.32e-12 64.3
Msa1334730 MtrunA17_Chr4g0018421 41.333 75 43 1 1 75 1 74 3.71e-12 64.7
Msa1334730 MtrunA17_Chr4g0074101 40.260 77 44 1 1 77 1 75 8.60e-12 63.5
Msa1334730 MtrunA17_Chr1g0175601 36.709 79 48 1 1 79 1 77 1.13e-11 62.8
Msa1334730 MtrunA17_Chr8g0357771 28.485 165 83 4 1 161 1 134 1.31e-11 62.8
Msa1334730 MtrunA17_Chr3g0099421 39.326 89 49 3 2 89 34 118 1.58e-11 63.9
Msa1334730 MtrunA17_Chr4g0018951 31.538 130 74 3 14 133 24 148 1.73e-11 62.0
Msa1334730 MtrunA17_Chr8g0355091 38.202 89 50 3 2 89 9 93 2.15e-11 63.2
Msa1334730 MtrunA17_Chr8g0376821 46.296 54 29 0 1 54 1 54 2.26e-11 62.0
Msa1334730 MtrunA17_Chr1g0180591 37.662 77 46 1 1 77 1 75 2.62e-11 61.6
Msa1334730 MtrunA17_Chr5g0419601 39.744 78 45 1 1 78 8 83 2.70e-11 62.8
Msa1334730 MtrunA17_Chr1g0206701 38.202 89 50 3 2 89 9 93 2.76e-11 62.8
Msa1334730 MtrunA17_Chr2g0317451 38.043 92 54 2 2 93 9 97 2.92e-11 62.8
Msa1334730 MtrunA17_Chr2g0317481 38.043 92 54 2 2 93 9 97 3.24e-11 62.4
Msa1334730 MtrunA17_Chr3g0099411 38.202 89 50 3 2 89 9 93 4.21e-11 62.0
Msa1334730 MtrunA17_Chr2g0292791 38.202 89 50 3 2 89 9 93 4.69e-11 62.0
Msa1334730 MtrunA17_Chr1g0169761 46.296 54 29 0 1 54 1 54 5.42e-11 60.8
Msa1334730 MtrunA17_Chr4g0052061 48.148 54 28 0 1 54 1 54 5.99e-11 60.1
Msa1334730 MtrunA17_Chr1g0206721 38.202 89 50 3 2 89 9 93 6.17e-11 61.6
Msa1334730 MtrunA17_Chr2g0325971 40.000 75 43 1 2 76 9 81 6.89e-11 61.6
Msa1334730 MtrunA17_Chr5g0422941 42.857 77 41 2 2 77 9 83 7.46e-11 61.6
Msa1334730 MtrunA17_Chr1g0188671 42.857 77 41 2 2 77 47 121 8.47e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334730 AT1G18750.4 48.173 301 111 7 1 270 1 287 1.17e-82 255
Msa1334730 AT1G18750.1 48.173 301 111 7 1 270 1 287 1.98e-82 255
Msa1334730 AT2G03060.3 45.614 285 105 6 1 250 1 270 4.25e-71 226
Msa1334730 AT2G03060.7 45.614 285 105 6 1 250 1 270 4.25e-71 226
Msa1334730 AT2G03060.1 45.614 285 105 6 1 250 1 270 4.25e-71 226
Msa1334730 AT2G03060.2 45.614 285 105 6 1 250 1 270 5.20e-71 226
Msa1334730 AT1G18750.3 42.216 334 115 11 1 270 1 320 6.12e-68 219
Msa1334730 AT1G69540.1 39.088 307 131 8 1 266 1 292 1.17e-57 190
Msa1334730 AT1G69540.2 35.526 380 173 13 1 329 1 359 1.21e-57 191
Msa1334730 AT1G18750.2 40.784 255 106 7 47 270 1 241 1.80e-51 174
Msa1334730 AT2G03060.6 34.804 204 83 6 82 250 24 212 3.45e-25 103
Msa1334730 AT2G03060.5 34.804 204 83 6 82 250 24 212 3.45e-25 103
Msa1334730 AT2G03060.4 34.804 204 83 6 82 250 24 212 3.45e-25 103
Msa1334730 AT1G77980.1 42.222 135 62 4 1 123 1 131 3.34e-24 101
Msa1334730 AT1G22130.1 55.696 79 34 1 1 79 1 78 3.05e-23 98.6
Msa1334730 AT1G77950.3 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1334730 AT1G77950.2 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1334730 AT1G77950.1 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1334730 AT1G77950.4 43.220 118 52 4 1 108 1 113 6.18e-22 93.6
Msa1334730 AT1G18750.6 34.337 166 90 6 110 270 46 197 6.13e-20 88.6
Msa1334730 AT1G18750.5 34.337 166 90 6 110 270 34 185 6.74e-20 88.6
Msa1334730 AT2G26320.1 47.561 82 43 0 1 82 17 98 3.36e-19 82.0
Msa1334730 AT5G13790.1 38.384 99 57 2 1 97 1 97 6.22e-16 77.0
Msa1334730 AT2G03710.1 37.762 143 74 5 1 133 1 138 7.40e-16 76.6
Msa1334730 AT2G03710.2 37.762 143 74 5 1 133 1 138 8.12e-16 76.6
Msa1334730 AT4G24540.1 47.222 72 36 1 1 72 1 70 9.44e-16 75.5
Msa1334730 AT5G23260.3 47.887 71 37 0 1 71 1 71 1.59e-15 75.5
Msa1334730 AT5G23260.2 47.887 71 37 0 1 71 1 71 1.60e-15 75.5
Msa1334730 AT5G23260.4 47.887 71 37 0 1 71 17 87 2.20e-15 75.5
Msa1334730 AT5G23260.1 47.887 71 37 0 1 71 17 87 2.27e-15 75.5
Msa1334730 AT3G57390.3 42.353 85 47 1 1 85 1 83 2.81e-15 74.3
Msa1334730 AT2G03710.3 37.762 143 74 5 1 133 1 138 2.85e-15 73.6
Msa1334730 AT3G57390.1 42.353 85 47 1 1 85 1 83 3.65e-15 74.7
Msa1334730 AT4G09960.2 30.000 150 91 4 1 143 1 143 4.25e-15 73.6
Msa1334730 AT5G13790.2 43.056 72 39 1 1 72 1 70 4.81e-15 74.7
Msa1334730 AT3G61120.1 29.388 245 138 9 1 235 1 220 5.74e-15 73.9
Msa1334730 AT3G57230.2 44.444 72 38 1 1 72 1 70 6.71e-15 71.2
Msa1334730 AT4G09960.1 29.268 164 97 5 1 157 1 152 7.18e-15 73.2
Msa1334730 AT4G09960.5 29.268 164 97 5 1 157 11 162 7.67e-15 73.6
Msa1334730 AT4G09960.6 29.268 164 97 5 1 157 2 153 8.62e-15 73.2
Msa1334730 AT2G45660.2 31.056 161 97 5 1 154 1 154 1.41e-14 70.9
Msa1334730 AT1G26310.1 43.038 79 44 1 1 79 1 78 1.78e-14 72.8
Msa1334730 AT2G45660.1 31.250 160 96 5 1 153 1 153 1.81e-14 72.0
Msa1334730 AT3G57230.1 44.444 72 38 1 1 72 1 70 2.07e-14 72.4
Msa1334730 AT3G57230.4 44.444 72 38 1 1 72 1 70 2.07e-14 72.4
Msa1334730 AT2G14210.1 43.590 78 43 1 1 78 1 77 2.13e-14 72.0
Msa1334730 AT2G22540.3 45.714 70 36 1 1 70 1 68 2.19e-14 72.0
Msa1334730 AT2G22540.1 45.714 70 36 1 1 70 1 68 2.19e-14 72.0
Msa1334730 AT2G14210.2 43.590 78 43 1 1 78 1 77 2.31e-14 72.0
Msa1334730 AT4G09960.3 29.268 164 97 5 1 157 27 178 2.48e-14 72.4
Msa1334730 AT4G11880.2 29.897 194 110 9 1 187 1 175 2.53e-14 70.9
Msa1334730 AT4G11880.3 29.897 194 110 9 1 187 1 175 2.53e-14 70.9
Msa1334730 AT4G37940.3 45.070 71 37 1 1 71 1 69 2.61e-14 70.5
Msa1334730 AT5G51870.2 45.333 75 39 1 1 75 1 73 3.46e-14 70.1
Msa1334730 AT4G37940.4 45.070 71 37 1 1 71 1 69 3.48e-14 71.2
Msa1334730 AT4G37435.1 45.070 71 38 1 1 71 1 70 3.57e-14 70.5
Msa1334730 AT4G37940.1 45.070 71 37 1 1 71 1 69 4.23e-14 71.2
Msa1334730 AT1G69120.2 43.038 79 44 1 1 79 1 78 4.54e-14 71.2
Msa1334730 AT2G22540.2 52.830 53 25 0 1 53 1 53 5.38e-14 70.9
Msa1334730 AT1G69120.1 43.038 79 44 1 1 79 1 78 6.27e-14 71.2
Msa1334730 AT5G51870.1 45.333 75 39 1 1 75 1 73 7.81e-14 70.1
Msa1334730 AT5G51870.5 34.932 146 72 6 1 132 24 160 8.91e-14 69.7
Msa1334730 AT3G58780.2 52.830 53 25 0 1 53 16 68 9.33e-14 70.5
Msa1334730 AT3G58780.1 52.830 53 25 0 1 53 16 68 1.14e-13 70.1
Msa1334730 AT2G42830.2 52.830 53 25 0 1 53 16 68 1.15e-13 70.1
Msa1334730 AT2G42830.1 52.830 53 25 0 1 53 16 68 1.17e-13 70.1
Msa1334730 AT1G71692.1 42.500 80 45 1 1 80 1 79 1.19e-13 69.3
Msa1334730 AT3G58780.5 52.830 53 25 0 1 53 22 74 1.25e-13 70.1
Msa1334730 AT3G54340.1 29.008 131 90 2 1 131 1 128 1.34e-13 69.7
Msa1334730 AT5G51870.4 45.333 75 39 1 1 75 1 73 1.36e-13 69.3
Msa1334730 AT5G10140.4 32.000 175 82 9 1 163 1 150 1.52e-13 68.6
Msa1334730 AT4G09960.4 29.878 164 96 6 1 157 96 247 1.55e-13 70.9
Msa1334730 AT5G51870.3 45.333 75 39 1 1 75 11 83 1.59e-13 69.3
Msa1334730 AT3G58780.3 52.830 53 25 0 1 53 16 68 1.87e-13 70.1
Msa1334730 AT4G18960.1 53.846 52 24 0 2 53 18 69 1.98e-13 69.7
Msa1334730 AT2G22630.1 43.750 80 41 2 1 78 1 78 2.65e-13 68.9
Msa1334730 AT2G22630.2 43.750 80 41 2 1 78 1 78 2.65e-13 68.9
Msa1334730 AT3G58780.4 52.830 53 25 0 1 53 16 68 2.68e-13 69.7
Msa1334730 AT5G20240.2 50.943 53 26 0 1 53 1 53 2.98e-13 67.0
Msa1334730 AT5G60910.1 34.351 131 77 4 1 123 1 130 3.04e-13 68.9
Msa1334730 AT5G10140.1 30.573 157 78 6 1 145 1 138 3.46e-13 67.8
Msa1334730 AT5G10140.2 52.830 53 25 0 1 53 1 53 3.98e-13 67.0
Msa1334730 AT5G62165.14 40.789 76 43 1 1 76 1 74 3.98e-13 65.5
Msa1334730 AT5G20240.1 50.943 53 26 0 1 53 1 53 4.20e-13 67.8
Msa1334730 AT5G10140.3 52.830 53 25 0 1 53 1 53 5.10e-13 67.0
Msa1334730 AT3G30260.1 40.845 71 41 1 1 71 1 70 5.17e-13 68.6
Msa1334730 AT2G45650.1 52.830 53 25 0 1 53 1 53 8.03e-13 67.8
Msa1334730 AT5G62165.3 30.247 162 99 4 1 155 1 155 1.17e-12 66.6
Msa1334730 AT5G62165.1 30.247 162 99 4 1 155 1 155 1.17e-12 66.6
Msa1334730 AT5G62165.2 30.247 162 99 4 1 155 1 155 1.17e-12 66.6
Msa1334730 AT4G22950.3 30.435 161 99 5 1 155 1 154 1.67e-12 66.2
Msa1334730 AT4G22950.1 30.435 161 99 5 1 155 1 154 1.67e-12 66.2
Msa1334730 AT4G11880.4 30.921 152 91 5 1 145 1 145 1.99e-12 66.2
Msa1334730 AT4G11880.1 30.921 152 91 5 1 145 1 145 1.99e-12 66.2
Msa1334730 AT5G62165.17 30.247 162 99 4 1 155 1 155 2.08e-12 64.7
Msa1334730 AT5G62165.12 30.247 162 99 4 1 155 1 155 2.08e-12 64.7
Msa1334730 AT5G62165.11 30.247 162 99 4 1 155 1 155 2.08e-12 64.7
Msa1334730 AT1G24260.3 50.943 53 26 0 1 53 1 53 2.13e-12 66.2
Msa1334730 AT1G24260.2 50.943 53 26 0 1 53 1 53 2.69e-12 66.2
Msa1334730 AT1G24260.1 50.943 53 26 0 1 53 1 53 2.71e-12 66.2
Msa1334730 AT5G62165.6 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1334730 AT5G62165.9 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1334730 AT5G62165.7 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1334730 AT5G62165.8 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1334730 AT5G62165.13 40.789 76 43 1 1 76 1 74 2.77e-12 64.3
Msa1334730 AT5G15800.1 50.943 53 26 0 1 53 1 53 2.96e-12 66.2
Msa1334730 AT5G15800.2 50.943 53 26 0 1 53 1 53 3.83e-12 65.9
Msa1334730 AT5G62165.10 40.789 76 43 1 1 76 1 74 4.02e-12 64.7
Msa1334730 AT5G62165.4 40.789 76 43 1 1 76 1 74 4.02e-12 64.7
Msa1334730 AT3G02310.2 50.943 53 26 0 1 53 1 53 5.06e-12 64.7
Msa1334730 AT4G22950.2 30.435 161 99 5 1 155 1 154 5.07e-12 63.9
Msa1334730 AT5G51860.2 42.466 73 40 1 1 73 1 71 5.81e-12 64.3
Msa1334730 AT3G02310.1 50.943 53 26 0 1 53 1 53 5.92e-12 65.5
Msa1334730 AT5G51860.1 42.466 73 40 1 1 73 1 71 6.01e-12 64.7
Msa1334730 AT5G65050.2 50.943 53 26 0 1 53 1 53 8.21e-12 63.5
Msa1334730 AT2G34440.1 26.415 159 101 3 1 153 1 149 1.21e-11 62.8
Msa1334730 AT5G65050.3 43.662 71 39 1 1 71 1 70 1.70e-11 63.2
Msa1334730 AT5G65050.1 43.662 71 39 1 1 71 1 70 1.83e-11 62.8
Msa1334730 AT5G65060.2 30.519 154 89 3 1 154 1 136 1.85e-11 62.8
Msa1334730 AT5G65050.5 43.662 71 39 1 1 71 1 70 2.02e-11 62.8
Msa1334730 AT5G65070.4 47.170 53 28 0 1 53 1 53 2.03e-11 61.6
Msa1334730 AT5G65070.1 47.170 53 28 0 1 53 1 53 3.59e-11 62.0
Msa1334730 AT3G66656.1 46.296 54 29 0 1 54 1 54 3.59e-11 61.6
Msa1334730 AT1G01530.1 48.148 54 28 0 1 54 6 59 4.11e-11 62.8
Msa1334730 AT4G36590.1 43.662 71 38 1 2 72 7 75 5.68e-11 62.4
Msa1334730 AT5G65070.5 47.170 53 28 0 1 53 1 53 5.88e-11 61.6
Msa1334730 AT5G65070.3 47.170 53 28 0 1 53 1 53 5.98e-11 62.0
Msa1334730 AT5G65070.2 47.170 53 28 0 1 53 1 53 6.62e-11 62.0

Find 77 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCCACATGTCACTATTT+TGG 0.102186 8_4:+55231201 Msa1334730:CDS
TAAATTGAAGATAAAGAAAT+TGG 0.209657 8_4:+55228766 Msa1334730:CDS
GTTCTAATTGAGCAGGAAAA+TGG 0.237423 8_4:+55229434 Msa1334730:intron
CCAAGCTATTCTGGTTATAC+TGG 0.263985 8_4:+55231154 Msa1334730:CDS
TGCACATTTAAGTAAGGATT+TGG 0.267580 8_4:-55231262 None:intergenic
CCAAAATCACTATTTGAAAA+TGG 0.281681 8_4:+55231403 Msa1334730:CDS
TTGAAAATGGACACCAGTTC+TGG 0.282537 8_4:+55231416 Msa1334730:CDS
AACCAAAGTTTATGTCATTC+AGG 0.285801 8_4:+55230862 Msa1334730:CDS
CCCTTTCTTGTGGGCTAAGT+TGG 0.290811 8_4:-55229335 None:intergenic
TTGTAGACCAGACATCATTA+AGG 0.306825 8_4:-55230778 None:intergenic
ATTTCACTCCCAAGCTATTC+TGG 0.316741 8_4:+55231145 Msa1334730:CDS
GGTACCTTGAATGAGTTAAA+TGG 0.322272 8_4:+55231235 Msa1334730:CDS
GCCCACAAGAAAGGGCTAAA+AGG 0.341342 8_4:+55229343 Msa1334730:CDS
CGCATTCAAGTGACATTAGT+TGG 0.358372 8_4:-55230641 None:intergenic
TGTAAAGATACAAGATTTCC+TGG 0.370430 8_4:+55229621 Msa1334730:CDS
CCTTTCTTGTGGGCTAAGTT+GGG 0.379085 8_4:-55229334 None:intergenic
AGGAAAGCCTACGTTATTAC+AGG 0.394098 8_4:+55228907 Msa1334730:CDS
CACTGTTGCACATTTAAGTA+AGG 0.404816 8_4:-55231268 None:intergenic
AAGGGCAAAGTCATCCCTTC+TGG 0.405073 8_4:-55230759 None:intergenic
TCTCAGTTATTGGAGTAATC+TGG 0.414099 8_4:+55230393 Msa1334730:intron
ACTTTGCCCTTAATGATGTC+TGG 0.425266 8_4:+55230771 Msa1334730:CDS
ATCATGATCCAATTTCTTGA+AGG 0.431577 8_4:-55229599 None:intergenic
CCCAACTTAGCCCACAAGAA+AGG 0.433847 8_4:+55229334 Msa1334730:CDS
CTTGGAACATCTTAGACAAA+TGG 0.437086 8_4:+55230429 Msa1334730:CDS
GTTCACTAGGTAACATCAAC+TGG 0.439721 8_4:-55230842 None:intergenic
TGTAGACCAGACATCATTAA+GGG 0.442310 8_4:-55230777 None:intergenic
TTGATTTGCTATTGATTGGT+TGG 0.442413 8_4:-55231342 None:intergenic
CACCTGAATGACATAAACTT+TGG 0.443645 8_4:-55230864 None:intergenic
CAATCGATTGTCTATACAAA+AGG 0.445203 8_4:+55230474 Msa1334730:CDS
TTATTTGATTTGCTATTGAT+TGG 0.458324 8_4:-55231346 None:intergenic
TTCACTTCCTCAAGATCTTG+TGG 0.459942 8_4:-55231313 None:intergenic
GATCATAATTGTTATTAACT+TGG 0.470376 8_4:-55231378 None:intergenic
AAGAATCTCAACCTTTGTCT+TGG 0.471192 8_4:+55230799 Msa1334730:CDS
TACAAGATTTCCTGGGTTCA+AGG 0.476006 8_4:+55229629 Msa1334730:CDS
ATTTCCATTTAACTCATTCA+AGG 0.477610 8_4:-55231239 None:intergenic
TTATACTGGGAACTGCAAAC+TGG 0.478109 8_4:+55231168 Msa1334730:CDS
TGGGAACTGCAAACTGGAGG+TGG 0.480619 8_4:+55231174 Msa1334730:CDS
CAGTATAACCAGAATAGCTT+GGG 0.488834 8_4:-55231153 None:intergenic
TCCTCAAGATCTTGTGGAGG+AGG 0.493605 8_4:-55231307 None:intergenic
CCAACTTAGCCCACAAGAAA+GGG 0.507371 8_4:+55229335 Msa1334730:CDS
CCAGTATAACCAGAATAGCT+TGG 0.510057 8_4:-55231154 None:intergenic
CATTTGTAGAATTCCAGAAC+TGG 0.522572 8_4:-55231429 None:intergenic
GTCACTTGAATGCGTTAACC+AGG 0.524046 8_4:+55230650 Msa1334730:CDS
CAGTTCTGGAATTCTACAAA+TGG 0.526268 8_4:+55231430 Msa1334730:CDS
TCTGGAGAAGATCAGTAACT+TGG 0.527978 8_4:+55230411 Msa1334730:CDS
ACTTATTCGAAGCGGAAAAG+TGG 0.527998 8_4:+55228812 Msa1334730:CDS
AAAACTACATGCTTACCCTA+TGG 0.529353 8_4:-55231489 None:intergenic
ATTGCTATGTATAACGAGAA+TGG 0.533675 8_4:+55231463 Msa1334730:CDS
CAAGCTATTCTGGTTATACT+GGG 0.535943 8_4:+55231155 Msa1334730:CDS
GACAAATGGAAGACTCACTC+AGG 0.540615 8_4:+55230443 Msa1334730:CDS
CCTTTGTCTTGGCTTCTAAG+TGG 0.542337 8_4:+55230810 Msa1334730:CDS
CCCTCCTCCACAAGATCTTG+AGG 0.562025 8_4:+55231306 Msa1334730:CDS
ATAACGAGAATGGTTACCAT+AGG 0.564017 8_4:+55231473 Msa1334730:CDS
ATTGATTCATAGTTACCAGA+AGG 0.565773 8_4:+55230744 Msa1334730:intron
CTGTTGGAGAAAACATGAGA+AGG 0.576749 8_4:-55228886 None:intergenic
AATTCTACAAATGGCTCTTG+TGG 0.581991 8_4:+55231439 Msa1334730:CDS
CTTATTCGAAGCGGAAAAGT+GGG 0.585417 8_4:+55228813 Msa1334730:CDS
CAATCACTTACCTTGAACCC+AGG 0.589742 8_4:-55229639 None:intergenic
CGTTCTCCCTGTAATAACGT+AGG 0.597125 8_4:-55228914 None:intergenic
GTAAAGATACAAGATTTCCT+GGG 0.597719 8_4:+55229622 Msa1334730:CDS
TAACGAGAATGGTTACCATA+GGG 0.597927 8_4:+55231474 Msa1334730:CDS
CCTCAAGATCTTGTGGAGGA+GGG 0.600496 8_4:-55231306 None:intergenic
GACATAAACTTTGGTTCACT+AGG 0.613858 8_4:-55230855 None:intergenic
CAATAGCAAATCAAATAACG+TGG 0.614557 8_4:+55231351 Msa1334730:CDS
ACTTCCTCAAGATCTTGTGG+AGG 0.620137 8_4:-55231310 None:intergenic
GGGAACTGCAAACTGGAGGT+GGG 0.623032 8_4:+55231175 Msa1334730:CDS
AATAACGTAGGCTTTCCTGT+TGG 0.623309 8_4:-55228902 None:intergenic
TTGATTCATAGTTACCAGAA+GGG 0.636080 8_4:+55230745 Msa1334730:intron
ATAGCTTGGGAGTGAAATGT+CGG 0.637360 8_4:-55231140 None:intergenic
TACTGGGAACTGCAAACTGG+AGG 0.648889 8_4:+55231171 Msa1334730:CDS
TGACCCAAAATAGTGACATG+TGG 0.657881 8_4:-55231205 None:intergenic
GCTTCGAATAAGTCACATGT+CGG 0.667125 8_4:-55228802 None:intergenic
ACAAATGGAAGACTCACTCA+GGG 0.670210 8_4:+55230444 Msa1334730:CDS
GGAAAGCCTACGTTATTACA+GGG 0.679926 8_4:+55228908 Msa1334730:CDS
GACATGTGACTTATTCGAAG+CGG 0.683369 8_4:+55228804 Msa1334730:CDS
CCACTTAGAAGCCAAGACAA+AGG 0.694579 8_4:-55230810 None:intergenic
CGTTATTACAGGGAGAACGA+AGG 0.718082 8_4:+55228918 Msa1334730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTCAAATATTTTAAAAAAA+TGG + chr8_4:55229120-55229139 Msa1334730:intron 10.0%
!!! AGTATATCTATTTTTTTTTA+AGG - chr8_4:55229183-55229202 None:intergenic 10.0%
!! ATAATAATAGATAGAAAAAA+GGG - chr8_4:55230694-55230713 None:intergenic 10.0%
!! TATAATAATAGATAGAAAAA+AGG - chr8_4:55230695-55230714 None:intergenic 10.0%
!! TAAATTGAAGATAAAGAAAT+TGG + chr8_4:55228766-55228785 Msa1334730:CDS 15.0%
!! AAAGTAATATATGAATTACA+AGG - chr8_4:55229489-55229508 None:intergenic 15.0%
!! TTTATTCACAAATTATCAAA+TGG + chr8_4:55229759-55229778 Msa1334730:intron 15.0%
!! TTATTCACAAATTATCAAAT+GGG + chr8_4:55229760-55229779 Msa1334730:intron 15.0%
!!! TATTGATATTTTGATTTCAT+CGG + chr8_4:55230598-55230617 Msa1334730:intron 15.0%
!!! ATTTTTGAAATAATCTTATC+CGG + chr8_4:55230715-55230734 Msa1334730:intron 15.0%
!!! ACTTATCATTTTAATCTCAA+AGG + chr8_4:55229092-55229111 Msa1334730:intron 20.0%
!!! ATTTTATTTTTCCAGCAATA+TGG + chr8_4:55229290-55229309 Msa1334730:intron 20.0%
!!! TTTGGTCTTGTTTAAAATTT+AGG + chr8_4:55229564-55229583 Msa1334730:intron 20.0%
!! AATCTTCAAAATCATTTACA+AGG - chr8_4:55229689-55229708 None:intergenic 20.0%
!! TGCAATATGAAAATTAAAAC+AGG - chr8_4:55229716-55229735 None:intergenic 20.0%
!! CGAAAAATTTAAATTGAACA+AGG - chr8_4:55230090-55230109 None:intergenic 20.0%
!! TGATAGTATTTGACATAATT+TGG - chr8_4:55230329-55230348 None:intergenic 20.0%
!! TGTCAAATACTATCATAATT+TGG + chr8_4:55230334-55230353 Msa1334730:intron 20.0%
!! GTCAAATACTATCATAATTT+GGG + chr8_4:55230335-55230354 Msa1334730:intron 20.0%
!! ATATGTAATATCTCAGTTAT+TGG + chr8_4:55230383-55230402 Msa1334730:intron 20.0%
!! TCTATACAAAAGGTAATATT+TGG + chr8_4:55230484-55230503 Msa1334730:intron 20.0%
!! AAAATACACTATTATTTACC+TGG - chr8_4:55230671-55230690 None:intergenic 20.0%
!!! TTTTTGAAATAATCTTATCC+GGG + chr8_4:55230716-55230735 Msa1334730:intron 20.0%
!!! TTATTTGATTTGCTATTGAT+TGG - chr8_4:55231349-55231368 None:intergenic 20.0%
!!! GATCATAATTGTTATTAACT+TGG - chr8_4:55231381-55231400 None:intergenic 20.0%
! CGAAGGTAATGTAATAATAA+CGG + chr8_4:55228935-55228954 Msa1334730:intron 25.0%
!! TTTCTCATTTTATCACAAGA+AGG - chr8_4:55229010-55229029 None:intergenic 25.0%
! CTTGTGATAAAATGAGAAAA+TGG + chr8_4:55229011-55229030 Msa1334730:intron 25.0%
!!! ATCATTTTAATCTCAAAGGA+CGG + chr8_4:55229096-55229115 Msa1334730:intron 25.0%
!!! ACTTTTACACACTTTAAAGA+TGG - chr8_4:55229221-55229240 None:intergenic 25.0%
! TCAGTCATTGCTTAAAAATT+TGG + chr8_4:55229546-55229565 Msa1334730:intron 25.0%
! GAAAAAAACCTTCAAGAAAT+TGG + chr8_4:55229591-55229610 Msa1334730:CDS 25.0%
! ATGAATAATAGTTCAACAAG+AGG - chr8_4:55229984-55230003 None:intergenic 25.0%
! TACACACTCATAATTAATAC+AGG - chr8_4:55230055-55230074 None:intergenic 25.0%
!!! CGACAAACACATTTTTTTTT+GGG - chr8_4:55230262-55230281 None:intergenic 25.0%
!!! TCGACAAACACATTTTTTTT+TGG - chr8_4:55230263-55230282 None:intergenic 25.0%
!! AGTTTGTACCAAAAAAGTAT+AGG - chr8_4:55230288-55230307 None:intergenic 25.0%
!! TAATCACAACGATTTTCTAA+GGG - chr8_4:55230559-55230578 None:intergenic 25.0%
!! TTAATCACAACGATTTTCTA+AGG - chr8_4:55230560-55230579 None:intergenic 25.0%
!! AAAGAGCTTTATAACAAAAG+TGG + chr8_4:55230913-55230932 Msa1334730:intron 25.0%
! ATTTCCATTTAACTCATTCA+AGG - chr8_4:55231242-55231261 None:intergenic 25.0%
!! CCATTTTCAAATAGTGATTT+TGG - chr8_4:55231406-55231425 None:intergenic 25.0%
! CCAAAATCACTATTTGAAAA+TGG + chr8_4:55231403-55231422 Msa1334730:CDS 25.0%
TTAAAGTGTGTAAAAGTCGA+AGG + chr8_4:55229224-55229243 Msa1334730:intron 30.0%
!! TTATTTTTCCAGCAATATGG+AGG + chr8_4:55229293-55229312 Msa1334730:intron 30.0%
! TTTTCTTGTTCTAATTGAGC+AGG + chr8_4:55229427-55229446 Msa1334730:intron 30.0%
ATCATGATCCAATTTCTTGA+AGG - chr8_4:55229602-55229621 None:intergenic 30.0%
TGTAAAGATACAAGATTTCC+TGG + chr8_4:55229621-55229640 Msa1334730:CDS 30.0%
GTAAAGATACAAGATTTCCT+GGG + chr8_4:55229622-55229641 Msa1334730:CDS 30.0%
!! ATTGCTTTAGAATCTTAAGC+TGG + chr8_4:55229789-55229808 Msa1334730:intron 30.0%
AGAATCTTAAGCTGGTAATT+AGG + chr8_4:55229797-55229816 Msa1334730:intron 30.0%
CAATTGAACTCATAACACTT+TGG - chr8_4:55229826-55229845 None:intergenic 30.0%
ATACAAAATTGGTAAGGTAG+AGG - chr8_4:55230011-55230030 None:intergenic 30.0%
AAGCACATACAAAATTGGTA+AGG - chr8_4:55230017-55230036 None:intergenic 30.0%
TTGCAAAGCACATACAAAAT+TGG - chr8_4:55230022-55230041 None:intergenic 30.0%
!!! TTTTTTGGGTGGTGTTTATT+TGG - chr8_4:55230248-55230267 None:intergenic 30.0%
!!! CAAACACATTTTTTTTTGGG+TGG - chr8_4:55230259-55230278 None:intergenic 30.0%
!!! TGTCGAAACCTATACTTTTT+TGG + chr8_4:55230277-55230296 Msa1334730:intron 30.0%
CAATCGATTGTCTATACAAA+AGG + chr8_4:55230474-55230493 Msa1334730:CDS 30.0%
! AGGTAATATTTGGTTTCATG+CGG + chr8_4:55230494-55230513 Msa1334730:intron 30.0%
! AATCACAACGATTTTCTAAG+GGG - chr8_4:55230558-55230577 None:intergenic 30.0%
ACTATGAATCAATATCATCC+CGG - chr8_4:55230737-55230756 None:intergenic 30.0%
! ATTGATTCATAGTTACCAGA+AGG + chr8_4:55230744-55230763 Msa1334730:intron 30.0%
TTGATTCATAGTTACCAGAA+GGG + chr8_4:55230745-55230764 Msa1334730:intron 30.0%
AACCAAAGTTTATGTCATTC+AGG + chr8_4:55230862-55230881 Msa1334730:CDS 30.0%
TGCACATTTAAGTAAGGATT+TGG - chr8_4:55231265-55231284 None:intergenic 30.0%
!! TTGATTTGCTATTGATTGGT+TGG - chr8_4:55231345-55231364 None:intergenic 30.0%
CAATAGCAAATCAAATAACG+TGG + chr8_4:55231351-55231370 Msa1334730:CDS 30.0%
ATTGCTATGTATAACGAGAA+TGG + chr8_4:55231463-55231482 Msa1334730:CDS 30.0%
GTTCTAATTGAGCAGGAAAA+TGG + chr8_4:55229434-55229453 Msa1334730:intron 35.0%
! AGATTCTCACTTGATGACTT+AGG - chr8_4:55229861-55229880 None:intergenic 35.0%
AAGTGAGAATCTTACAAGCT+CGG + chr8_4:55229869-55229888 Msa1334730:intron 35.0%
!! GTTTATTTGGTGTGAGCTAA+AGG - chr8_4:55230235-55230254 None:intergenic 35.0%
TCTCAGTTATTGGAGTAATC+TGG + chr8_4:55230393-55230412 Msa1334730:intron 35.0%
CTTGGAACATCTTAGACAAA+TGG + chr8_4:55230429-55230448 Msa1334730:CDS 35.0%
!!! GATATTTTGATTTCATCGGC+AGG + chr8_4:55230602-55230621 Msa1334730:intron 35.0%
TGTAGACCAGACATCATTAA+GGG - chr8_4:55230780-55230799 None:intergenic 35.0%
TTGTAGACCAGACATCATTA+AGG - chr8_4:55230781-55230800 None:intergenic 35.0%
AAGAATCTCAACCTTTGTCT+TGG + chr8_4:55230799-55230818 Msa1334730:CDS 35.0%
! GACATAAACTTTGGTTCACT+AGG - chr8_4:55230858-55230877 None:intergenic 35.0%
CACCTGAATGACATAAACTT+TGG - chr8_4:55230867-55230886 None:intergenic 35.0%
CAGTATAACCAGAATAGCTT+GGG - chr8_4:55231156-55231175 None:intergenic 35.0%
! CAAGCTATTCTGGTTATACT+GGG + chr8_4:55231155-55231174 Msa1334730:CDS 35.0%
! TCTCCACATGTCACTATTTT+GGG + chr8_4:55231202-55231221 Msa1334730:CDS 35.0%
GGTACCTTGAATGAGTTAAA+TGG + chr8_4:55231235-55231254 Msa1334730:CDS 35.0%
CACTGTTGCACATTTAAGTA+AGG - chr8_4:55231271-55231290 None:intergenic 35.0%
CATTTGTAGAATTCCAGAAC+TGG - chr8_4:55231432-55231451 None:intergenic 35.0%
!! CAGTTCTGGAATTCTACAAA+TGG + chr8_4:55231430-55231449 Msa1334730:CDS 35.0%
AATTCTACAAATGGCTCTTG+TGG + chr8_4:55231439-55231458 Msa1334730:CDS 35.0%
ATAACGAGAATGGTTACCAT+AGG + chr8_4:55231473-55231492 Msa1334730:CDS 35.0%
TAACGAGAATGGTTACCATA+GGG + chr8_4:55231474-55231493 Msa1334730:CDS 35.0%
GCTTCGAATAAGTCACATGT+CGG - chr8_4:55228805-55228824 None:intergenic 40.0%
GACATGTGACTTATTCGAAG+CGG + chr8_4:55228804-55228823 Msa1334730:CDS 40.0%
ACTTATTCGAAGCGGAAAAG+TGG + chr8_4:55228812-55228831 Msa1334730:CDS 40.0%
CTTATTCGAAGCGGAAAAGT+GGG + chr8_4:55228813-55228832 Msa1334730:CDS 40.0%
CTGTTGGAGAAAACATGAGA+AGG - chr8_4:55228889-55228908 None:intergenic 40.0%
! CTTCTCATGTTTTCTCCAAC+AGG + chr8_4:55228887-55228906 Msa1334730:CDS 40.0%
AATAACGTAGGCTTTCCTGT+TGG - chr8_4:55228905-55228924 None:intergenic 40.0%
AGGAAAGCCTACGTTATTAC+AGG + chr8_4:55228907-55228926 Msa1334730:CDS 40.0%
GGAAAGCCTACGTTATTACA+GGG + chr8_4:55228908-55228927 Msa1334730:CDS 40.0%
AATGATCTCCTCCATATTGC+TGG - chr8_4:55229304-55229323 None:intergenic 40.0%
! ACCTTTTAGCCCTTTCTTGT+GGG - chr8_4:55229347-55229366 None:intergenic 40.0%
! AACCTTTTAGCCCTTTCTTG+TGG - chr8_4:55229348-55229367 None:intergenic 40.0%
TACAAGATTTCCTGGGTTCA+AGG + chr8_4:55229629-55229648 Msa1334730:CDS 40.0%
ATATTGCAGCAGTCAAACAG+TGG + chr8_4:55229728-55229747 Msa1334730:intron 40.0%
! TGAGTTCAATTGCCATTTGC+AGG + chr8_4:55229834-55229853 Msa1334730:intron 40.0%
CAGAAATACAACAGAGACTG+AGG + chr8_4:55229896-55229915 Msa1334730:intron 40.0%
TCTGGAGAAGATCAGTAACT+TGG + chr8_4:55230411-55230430 Msa1334730:CDS 40.0%
ACAAATGGAAGACTCACTCA+GGG + chr8_4:55230444-55230463 Msa1334730:CDS 40.0%
!! ACAACGATTTTCTAAGGGGA+CGG - chr8_4:55230554-55230573 None:intergenic 40.0%
CGCATTCAAGTGACATTAGT+TGG - chr8_4:55230644-55230663 None:intergenic 40.0%
ACTTTGCCCTTAATGATGTC+TGG + chr8_4:55230771-55230790 Msa1334730:CDS 40.0%
GTTCACTAGGTAACATCAAC+TGG - chr8_4:55230845-55230864 None:intergenic 40.0%
ATAGCTTGGGAGTGAAATGT+CGG - chr8_4:55231143-55231162 None:intergenic 40.0%
ATTTCACTCCCAAGCTATTC+TGG + chr8_4:55231145-55231164 Msa1334730:CDS 40.0%
CCAGTATAACCAGAATAGCT+TGG - chr8_4:55231157-55231176 None:intergenic 40.0%
! CCAAGCTATTCTGGTTATAC+TGG + chr8_4:55231154-55231173 Msa1334730:CDS 40.0%
TTATACTGGGAACTGCAAAC+TGG + chr8_4:55231168-55231187 Msa1334730:CDS 40.0%
! CTCTCCACATGTCACTATTT+TGG + chr8_4:55231201-55231220 Msa1334730:CDS 40.0%
TGACCCAAAATAGTGACATG+TGG - chr8_4:55231208-55231227 None:intergenic 40.0%
!! ACATGTCACTATTTTGGGTC+AGG + chr8_4:55231207-55231226 Msa1334730:CDS 40.0%
!! CATGTCACTATTTTGGGTCA+GGG + chr8_4:55231208-55231227 Msa1334730:CDS 40.0%
TTCACTTCCTCAAGATCTTG+TGG - chr8_4:55231316-55231335 None:intergenic 40.0%
TTGAAAATGGACACCAGTTC+TGG + chr8_4:55231416-55231435 Msa1334730:CDS 40.0%
CGTTCTCCCTGTAATAACGT+AGG - chr8_4:55228917-55228936 None:intergenic 45.0%
CGTTATTACAGGGAGAACGA+AGG + chr8_4:55228918-55228937 Msa1334730:CDS 45.0%
! CCTTTCTTGTGGGCTAAGTT+GGG - chr8_4:55229337-55229356 None:intergenic 45.0%
CCAACTTAGCCCACAAGAAA+GGG + chr8_4:55229335-55229354 Msa1334730:CDS 45.0%
CAATCACTTACCTTGAACCC+AGG - chr8_4:55229642-55229661 None:intergenic 45.0%
GATGACTTAGGTCCTGCAAA+TGG - chr8_4:55229849-55229868 None:intergenic 45.0%
!!! AGGTGCAACTTTGGAGCTTT+TGG + chr8_4:55229916-55229935 Msa1334730:intron 45.0%
! TTGGTGTGAGCTAAAGGCAT+GGG - chr8_4:55230229-55230248 None:intergenic 45.0%
! TTTGGTGTGAGCTAAAGGCA+TGG - chr8_4:55230230-55230249 None:intergenic 45.0%
GACAAATGGAAGACTCACTC+AGG + chr8_4:55230443-55230462 Msa1334730:CDS 45.0%
!! CAACGATTTTCTAAGGGGAC+GGG - chr8_4:55230553-55230572 None:intergenic 45.0%
GTCACTTGAATGCGTTAACC+AGG + chr8_4:55230650-55230669 Msa1334730:CDS 45.0%
CCACTTAGAAGCCAAGACAA+AGG - chr8_4:55230813-55230832 None:intergenic 45.0%
CCTTTGTCTTGGCTTCTAAG+TGG + chr8_4:55230810-55230829 Msa1334730:CDS 45.0%
ACTTCCTCAAGATCTTGTGG+AGG - chr8_4:55231313-55231332 None:intergenic 45.0%
!! AATAAAAATAAATATTTAGT+GGG - chr8_4:55229382-55229401 None:intergenic 5.0%
!! AAATAAAAATAAATATTTAG+TGG - chr8_4:55229383-55229402 None:intergenic 5.0%
CCCAACTTAGCCCACAAGAA+AGG + chr8_4:55229334-55229353 Msa1334730:CDS 50.0%
! CCCTTTCTTGTGGGCTAAGT+TGG - chr8_4:55229338-55229357 None:intergenic 50.0%
GCCCACAAGAAAGGGCTAAA+AGG + chr8_4:55229343-55229362 Msa1334730:CDS 50.0%
TTGCAGCAGTCAAACAGTGG+AGG + chr8_4:55229731-55229750 Msa1334730:intron 50.0%
CAGAGACTGAGGTGCAACTT+TGG + chr8_4:55229907-55229926 Msa1334730:intron 50.0%
! TGGTGTGAGCTAAAGGCATG+GGG - chr8_4:55230228-55230247 None:intergenic 50.0%
AAGGGCAAAGTCATCCCTTC+TGG - chr8_4:55230762-55230781 None:intergenic 50.0%
TACTGGGAACTGCAAACTGG+AGG + chr8_4:55231171-55231190 Msa1334730:CDS 50.0%
!! GTCACTATTTTGGGTCAGGG+AGG + chr8_4:55231211-55231230 Msa1334730:CDS 50.0%
!! ACTATTTTGGGTCAGGGAGG+TGG + chr8_4:55231214-55231233 Msa1334730:CDS 50.0%
CCTCAAGATCTTGTGGAGGA+GGG - chr8_4:55231309-55231328 None:intergenic 50.0%
TCCTCAAGATCTTGTGGAGG+AGG - chr8_4:55231310-55231329 None:intergenic 50.0%
! TTCTAAGGGGACGGGAACGA+TGG - chr8_4:55230545-55230564 None:intergenic 55.0%
TGGGAACTGCAAACTGGAGG+TGG + chr8_4:55231174-55231193 Msa1334730:CDS 55.0%
GGGAACTGCAAACTGGAGGT+GGG + chr8_4:55231175-55231194 Msa1334730:CDS 55.0%
CCCTCCTCCACAAGATCTTG+AGG + chr8_4:55231306-55231325 Msa1334730:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 55228755 55231507 55228755 ID=Msa1334730;Name=Msa1334730
chr8_4 mRNA 55228755 55231507 55228755 ID=Msa1334730-mRNA-1;Parent=Msa1334730;Name=Msa1334730-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0|0|0|1|0.85|1|8|0|333
chr8_4 exon 55228755 55228939 55228755 ID=Msa1334730-mRNA-1:exon:17243;Parent=Msa1334730-mRNA-1
chr8_4 exon 55229305 55229364 55229305 ID=Msa1334730-mRNA-1:exon:17244;Parent=Msa1334730-mRNA-1
chr8_4 exon 55229449 55229467 55229449 ID=Msa1334730-mRNA-1:exon:17245;Parent=Msa1334730-mRNA-1
chr8_4 exon 55229586 55229650 55229586 ID=Msa1334730-mRNA-1:exon:17246;Parent=Msa1334730-mRNA-1
chr8_4 exon 55230399 55230495 55230399 ID=Msa1334730-mRNA-1:exon:17247;Parent=Msa1334730-mRNA-1
chr8_4 exon 55230624 55230671 55230624 ID=Msa1334730-mRNA-1:exon:17248;Parent=Msa1334730-mRNA-1
chr8_4 exon 55230756 55230883 55230756 ID=Msa1334730-mRNA-1:exon:17249;Parent=Msa1334730-mRNA-1
chr8_4 exon 55231108 55231507 55231108 ID=Msa1334730-mRNA-1:exon:17250;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55228755 55228939 55228755 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55229305 55229364 55229305 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55229449 55229467 55229449 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55229586 55229650 55229586 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55230399 55230495 55230399 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55230624 55230671 55230624 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55230756 55230883 55230756 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
chr8_4 CDS 55231108 55231507 55231108 ID=Msa1334730-mRNA-1:cds;Parent=Msa1334730-mRNA-1
Gene Sequence

>Msa1334730

ATGGGGAGAGTTAAATTGAAGATAAAGAAATTGGAGAGCACAAGCAACCGACATGTGACTTATTCGAAGCGGAAAAGTGGGATCTTGAAGAAAGCAAAAGAGTTATCAATTCTATGTGATATAGATATTCTCCTTCTCATGTTTTCTCCAACAGGAAAGCCTACGTTATTACAGGGAGAACGAAGCAATATGGAGGAGATCATTGCAAAATTTGCCCAACTTAGCCCACAAGAAAGGGCTAAAAGGAAAATGGAAAGTCTTGAAGCACTGAAAAAAACCTTCAAGAAATTGGATCATGATGTAAAGATACAAGATTTCCTGGGTTCAAGTTATTGGAGTAATCTGGAGAAGATCAGTAACTTGGAACATCTTAGACAAATGGAAGACTCACTCAGGGAATCAATCAATCGATTGTCTATACAAAAGGAAAATCTCGAAAAGCACCAACTAATGTCACTTGAATGCGTTAACCAGTTACCAGAAGGGATGACTTTGCCCTTAATGATGTCTGGTCTACAAGAATCTCAACCTTTGTCTTGGCTTCTAAGTGGTGATAATCACCAGTTGATGTTACCTAGTGAACCAAAGTTTATGTCATTCAGTGACAATGTCAATAGAGATGTTGAGTGCTCAACCGACATTTCACTCCCAAGCTATTCTGGTTATACTGGGAACTGCAAACTGGAGGTGGGAAGCTCTCCACATGTCACTATTTTGGGTCAGGGAGGTGGTACCTTGAATGAGTTAAATGGAAATCCAAATCCTTACTTAAATGTGCAACAGTGTCAGCAGTTTGCATACCCTCCTCCACAAGATCTTGAGGAAGTGAAACATCACCAACCAATCAATAGCAAATCAAATAACGTGGACTACCAAGTTAATAACAATTATGATCTGCCAAAATCACTATTTGAAAATGGACACCAGTTCTGGAATTCTACAAATGGCTCTTGTGGTATTGCTATGTATAACGAGAATGGTTACCATAGGGTAAGCATGTAG
Protein sequence

>Msa1334730

MGRVKLKIKKLESTSNRHVTYSKRKSGILKKAKELSILCDIDILLLMFSPTGKPTLLQGERSNMEEIIAKFAQLSPQERAKRKMESLEALKKTFKKLDHDVKIQDFLGSSYWSNLEKISNLEHLRQMEDSLRESINRLSIQKENLEKHQLMSLECVNQLPEGMTLPLMMSGLQESQPLSWLLSGDNHQLMLPSEPKFMSFSDNVNRDVECSTDISLPSYSGYTGNCKLEVGSSPHVTILGQGGGTLNELNGNPNPYLNVQQCQQFAYPPPQDLEEVKHHQPINSKSNNVDYQVNNNYDLPKSLFENGHQFWNSTNGSCGIAMYNENGYHRVSM*