AlfalfaGEDB | Msa1334830

M. sativa cultivar zhongmu-4 / Msa1334830

KEGG: K04733
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1334830	AET01445.1	99.342	152	1	0	12	163	650	801	1.74e-98	311

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1334830	sp\|Q9LPZ9\|SD113_ARATH	52.632	152	72	0	12	163	679	830	2.21e-48	169

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1334830	G7LFU9	99.342	152	1	0	12	163	650	801	8.33e-99	311

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1334830	MtrunA17_Chr8g0340081	99.342	152	1	0	12	163	675	826	3.01e-102	311
Msa1334830	MtrunA17_Chr8g0340051	92.157	153	11	1	12	163	197	349	9.18e-101	293
Msa1334830	MtrunA17_Chr8g0340071	92.105	152	12	0	12	163	671	822	1.81e-94	290
Msa1334830	MtrunA17_Chr8g0340091	87.582	153	18	1	12	163	700	852	3.06e-90	280
Msa1334830	MtrunA17_Chr8g0340111	88.816	152	17	0	12	163	673	824	1.84e-83	261
Msa1334830	MtrunA17_Chr2g0280761	58.000	150	61	1	14	163	1	148	2.80e-61	186
Msa1334830	MtrunA17_Chr7g0236531	55.921	152	67	0	12	163	206	357	2.35e-58	185
Msa1334830	MtrunA17_Chr4g0055011	55.921	152	67	0	12	163	239	390	1.03e-57	184
Msa1334830	MtrunA17_Chr2g0280831	57.616	151	61	2	12	162	263	410	1.09e-57	185
Msa1334830	MtrunA17_Chr7g0236691	57.237	152	65	0	12	163	668	819	6.12e-56	187

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1334830	AT1G11350.1	52.632	152	72	0	12	163	679	830	2.25e-49	169
Msa1334830	AT1G11300.2	53.947	152	63	1	12	163	661	805	4.31e-48	166
Msa1334830	AT1G11300.1	53.947	152	63	1	12	163	676	820	4.35e-48	166
Msa1334830	AT1G11330.1	50.658	152	75	0	12	163	689	840	4.40e-48	166
Msa1334830	AT1G11330.2	50.658	152	75	0	12	163	691	842	4.84e-48	166
Msa1334830	AT1G11303.1	48.684	152	71	2	12	163	676	820	3.10e-42	149
Msa1334830	AT4G23150.1	48.299	147	74	1	12	158	503	647	1.93e-41	147
Msa1334830	AT1G61610.1	48.684	152	77	1	12	163	692	842	2.15e-41	147
Msa1334830	AT1G65790.1	45.570	158	80	3	12	163	686	843	1.72e-40	144
Msa1334830	AT4G23180.1	54.783	115	52	0	12	126	515	629	1.06e-39	142

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Find 39 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAACAGTTGATATACT+TGG	0.549330	8_4:+55503640	None:intergenic
AAAGATGCATTCCATACCTC+AGG	0.536675	8_4:+55503730	None:intergenic
AAATCAGATGTCTATAGTTT+TGG	0.296202	8_4:-55504135	Msa1334830:CDS
AACATATATCCTAAATGCAG+TGG	0.595883	8_4:-55504201	Msa1334830:CDS
AAGGCAACTTTACCTGGAGG+TGG	0.588366	8_4:+55503589	None:intergenic
AATCAATACTACCATTGTTA+AGG	0.441402	8_4:-55503450	Msa1334830:three_prime_UTR
ACAAAGGCAACTTTACCTGG+AGG	0.745641	8_4:+55503586	None:intergenic
ACCTAATACATCTTTGCATA+AGG	0.449033	8_4:-55503286	Msa1334830:three_prime_UTR
AGATTACTAGTTACCGGTAA+AGG	0.492836	8_4:+55503188	None:intergenic
AGCCCCTCCATTGCATATTC+AGG	0.339267	8_4:+55504168	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACATATATCCTAAATGCAG+TGG	-	chr8_4:55503213-55503232	Msa1334830:three_prime_UTR	30.0%
TTTGTTTCATTTACTAGGCA+TGG	-	chr8_4:55503622-55503641	Msa1334830:CDS	30.0%
TATATCTCTAATAGATCCTG+AGG	-	chr8_4:55503668-55503687	Msa1334830:CDS	30.0%
CAGGTAAATAATGTGCATTA+TGG	-	chr8_4:55503941-55503960	Msa1334830:intron	30.0%
TTGTATTACCTTGATTGTTG+TGG	-	chr8_4:55504072-55504091	Msa1334830:CDS	30.0%
ACCTAATACATCTTTGCATA+AGG	-	chr8_4:55504128-55504147	Msa1334830:CDS	30.0%
TCCTTATGCAAAGATGTATT+AGG	+	chr8_4:55504132-55504151	None:intergenic	30.0%
AACATATATCCTAAATGCAG+TGG	-	chr8_4:55503213-55503232	Msa1334830:three_prime_UTR	30.0%
TTTGTTTCATTTACTAGGCA+TGG	-	chr8_4:55503622-55503641	Msa1334830:CDS	30.0%
TATATCTCTAATAGATCCTG+AGG	-	chr8_4:55503668-55503687	Msa1334830:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	55503201	55504235	55503201	ID=Msa1334830;Name=Msa1334830
chr8_4	mRNA	55503201	55504235	55503201	ID=Msa1334830-mRNA-1;Parent=Msa1334830;Name=Msa1334830-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0\|1\|0.5\|1\|1\|1\|2\|288\|163
chr8_4	exon	55503201	55503797	55503201	ID=Msa1334830-mRNA-1:exon:17325;Parent=Msa1334830-mRNA-1
chr8_4	exon	55504053	55504235	55504053	ID=Msa1334830-mRNA-1:exon:17324;Parent=Msa1334830-mRNA-1
chr8_4	CDS	55504053	55504235	55504053	ID=Msa1334830-mRNA-1:cds;Parent=Msa1334830-mRNA-1
chr8_4	CDS	55503489	55503797	55503489	ID=Msa1334830-mRNA-1:cds;Parent=Msa1334830-mRNA-1
chr8_4	three_prime_UTR	55503201	55503488	55503201	ID=Msa1334830-mRNA-1:three_prime_utr;Parent=Msa1334830-mRNA-1

Gene Sequence

>Msa1334830

ATGGGTTTGTCTAACATATATCCTAAATGCAGTGGTTATATGCCACCTGAATATGCAATGGAGGGGCTTTTCTCAGAGAAATCAGATGTCTATAGTTTTGGAGTTTTATTGCTAGAGCTTGTCAGTGGACGAAGAAATAGTAGCTTTTATCACAGCGAGGATTCTCTTAGTCTTGTAGGCTTTGCATGGAAGCTATGGTTAGAAGAAAACATTATATCTCTAATAGATCCTGAGGTATGGAATGCATCTTTCGAGAGCAGCATGCTTAGGTGCATACACATAGGACTTCTTTGTGTGCAAGAACTTCCAAAAGAAAGACCAAGTATATCAACTGTTGTTTTGATGCTTATAAGTGAGATCACACATCTTCCACCTCCAGGTAAAGTTGCCTTTGTTCACAAGCAAAATTCTCGAAGTACAGAATCTTCTCAACAAAGTCATCGATCTAACTCAAACAACAATGTAACTATGAGCGATGTCACAGGCAGGTAA

Protein sequence

>Msa1334830

MGLSNIYPKCSGYMPPEYAMEGLFSEKSDVYSFGVLLLELVSGRRNSSFYHSEDSLSLVGFAWKLWLEENIISLIDPEVWNASFESSMLRCIHIGLLCVQELPKERPSISTVVLMLISEITHLPPPGKVAFVHKQNSRSTESSQQSHRSNSNNNVTMSDVTGR*