Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1334890 | KEH31863.1 | 100.000 | 68 | 0 | 0 | 1 | 68 | 1 | 68 | 4.57e-40 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1334890 | sp|Q8LSZ4|E2FE_ARATH | 78.947 | 57 | 12 | 0 | 12 | 68 | 29 | 85 | 1.53e-27 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1334890 | A0A072US47 | 100.000 | 68 | 0 | 0 | 1 | 68 | 1 | 68 | 2.18e-40 | 144 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1334890 | TF | E2F-DP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1334890 | MtrunA17_Chr4g0059551 | 100.000 | 68 | 0 | 0 | 1 | 68 | 1 | 68 | 6.90e-42 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1334890 | AT3G48160.1 | 78.947 | 57 | 12 | 0 | 12 | 68 | 29 | 85 | 1.39e-28 | 104 |
| Msa1334890 | AT3G48160.2 | 78.947 | 57 | 12 | 0 | 12 | 68 | 29 | 85 | 1.56e-28 | 104 |
| Msa1334890 | AT3G01330.1 | 75.000 | 56 | 14 | 0 | 13 | 68 | 17 | 72 | 5.71e-24 | 92.0 |
| Msa1334890 | AT5G14960.1 | 75.000 | 56 | 14 | 0 | 13 | 68 | 9 | 64 | 1.30e-22 | 88.6 |
Find 36 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGATGTTTGATGAATTA+AGG | 0.183601 | 8_4:-55680531 | None:intergenic |
| TGGATCTGAAATTCGAAATT+TGG | 0.191238 | 8_4:-55680564 | None:intergenic |
| AAAGACGATGTTCGTTTAAT+TGG | 0.211016 | 8_4:+55680778 | Msa1334890:CDS |
| CGATGATGTTGTTCGAATTA+AGG | 0.234552 | 8_4:-55680489 | None:intergenic |
| GACGATGCTGCCGCCAAATT+AGG | 0.253633 | 8_4:+55680805 | Msa1334890:CDS |
| GTAATAATGAATACCTAATT+TGG | 0.256592 | 8_4:-55680818 | None:intergenic |
| CAGAGCCGTTTAGATATAAA+AGG | 0.269147 | 8_4:-55680457 | None:intergenic |
| GGCCGAAATTTGAAAATTGA+TGG | 0.374089 | 8_4:-55680404 | None:intergenic |
| TTCATGGATAGATAGATAGA+TGG | 0.386470 | 8_4:-55680245 | None:intergenic |
| TCAATGTTCTCGAAAGCATC+GGG | 0.403367 | 8_4:+55680950 | Msa1334890:CDS |
| AATTTCGAATTTCAGATCCA+TGG | 0.497190 | 8_4:+55680567 | Msa1334890:exon |
| ACACTTACTTGGTGCATAAG+AGG | 0.500577 | 8_4:-55680654 | None:intergenic |
| AGAGCCGTTTAGATATAAAA+GGG | 0.513466 | 8_4:-55680456 | None:intergenic |
| AGAGGGTGTTTGTGTGTGGC+GGG | 0.533655 | 8_4:-55680425 | None:intergenic |
| ACTTGGTGCATAAGAGGCCG+AGG | 0.537885 | 8_4:-55680648 | None:intergenic |
| GTCAATGTTCTCGAAAGCAT+CGG | 0.541833 | 8_4:+55680949 | Msa1334890:CDS |
| TTGCGATTTGCGTTGGAGGG+AGG | 0.548686 | 8_4:-55680346 | None:intergenic |
| ATCTGAAATTCGAAATTTGG+AGG | 0.550384 | 8_4:-55680561 | None:intergenic |
| ATCGGAGGAAGAAGCAGCCA+TGG | 0.550392 | 8_4:-55680584 | None:intergenic |
| AAGAGGGTGTTTGTGTGTGG+CGG | 0.559507 | 8_4:-55680426 | None:intergenic |
| TCGTAAGTGTGGTGTCGTGA+AGG | 0.561541 | 8_4:-55680610 | None:intergenic |
| GGTAACCCTAATGGAAGTGA+TGG | 0.570404 | 8_4:+55680184 | None:intergenic |
| AGAATTCAAATACGAGGTTG+CGG | 0.578791 | 8_4:+55680212 | Msa1334890:five_prime_UTR |
| GTTCAGGTGTTGAGAGAAGA+CGG | 0.579277 | 8_4:+55680914 | Msa1334890:intron |
| TCTGAAATTCGAAATTTGGA+GGG | 0.584453 | 8_4:-55680560 | None:intergenic |
| ATAATGAATACCTAATTTGG+CGG | 0.587773 | 8_4:-55680815 | None:intergenic |
| TTTGTTTACGATCGTAAGTG+TGG | 0.596230 | 8_4:-55680621 | None:intergenic |
| GTGGTGTCGTGAAGGAGGAT+CGG | 0.598361 | 8_4:-55680602 | None:intergenic |
| CTTGGTGCATAAGAGGCCGA+GGG | 0.608163 | 8_4:-55680647 | None:intergenic |
| GAGAAGAGGGTGTTTGTGTG+TGG | 0.617453 | 8_4:-55680429 | None:intergenic |
| TAAGTGTGGTGTCGTGAAGG+AGG | 0.625428 | 8_4:-55680607 | None:intergenic |
| GTGTCGTGAAGGAGGATCGG+AGG | 0.668512 | 8_4:-55680599 | None:intergenic |
| GAGCCGTTTAGATATAAAAG+GGG | 0.668908 | 8_4:-55680455 | None:intergenic |
| AGCCGTTTAGATATAAAAGG+GGG | 0.695293 | 8_4:-55680454 | None:intergenic |
| CAATGTTCTCGAAAGCATCG+GGG | 0.717872 | 8_4:+55680951 | Msa1334890:CDS |
| GATAAGAGAATTCAAATACG+AGG | 0.763019 | 8_4:+55680206 | Msa1334890:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCACTAATTATTTATTGATT+CGG | + | chr8_4:55680722-55680741 | Msa1334890:intron | 15.0% |
| !!! | TCACTAATTATTTATTGATT+CGG | + | chr8_4:55680722-55680741 | Msa1334890:intron | 15.0% |
| !!! | TTTACTGTTTTGTTTTTTCA+TGG | - | chr8_4:55680264-55680283 | None:intergenic | 20.0% |
| !!! | TAAAATCTTTTTCAGTTAAG+CGG | - | chr8_4:55680300-55680319 | None:intergenic | 20.0% |
| !!! | TTCCATCAATTTTCAAATTT+CGG | + | chr8_4:55680402-55680421 | Msa1334890:five_prime_UTR | 20.0% |
| !! | GTAATAATGAATACCTAATT+TGG | - | chr8_4:55680821-55680840 | None:intergenic | 20.0% |
| !!! | TTTACTGTTTTGTTTTTTCA+TGG | - | chr8_4:55680264-55680283 | None:intergenic | 20.0% |
| !!! | TAAAATCTTTTTCAGTTAAG+CGG | - | chr8_4:55680300-55680319 | None:intergenic | 20.0% |
| !!! | TTCCATCAATTTTCAAATTT+CGG | + | chr8_4:55680402-55680421 | Msa1334890:five_prime_UTR | 20.0% |
| !! | GTAATAATGAATACCTAATT+TGG | - | chr8_4:55680821-55680840 | None:intergenic | 20.0% |
| !!! | AAAATCTTTTTCAGTTAAGC+GGG | - | chr8_4:55680299-55680318 | None:intergenic | 25.0% |
| !!! | GTTTTTTTGGATCCAAAAAT+TGG | - | chr8_4:55680374-55680393 | None:intergenic | 25.0% |
| ! | ATAATGAATACCTAATTTGG+CGG | - | chr8_4:55680818-55680837 | None:intergenic | 25.0% |
| !!! | AAAATCTTTTTCAGTTAAGC+GGG | - | chr8_4:55680299-55680318 | None:intergenic | 25.0% |
| !!! | GTTTTTTTGGATCCAAAAAT+TGG | - | chr8_4:55680374-55680393 | None:intergenic | 25.0% |
| ! | ATAATGAATACCTAATTTGG+CGG | - | chr8_4:55680818-55680837 | None:intergenic | 25.0% |
| GATAAGAGAATTCAAATACG+AGG | + | chr8_4:55680206-55680225 | Msa1334890:five_prime_UTR | 30.0% | |
| TTCATGGATAGATAGATAGA+TGG | - | chr8_4:55680248-55680267 | None:intergenic | 30.0% | |
| ! | CAAATCGCAAAACCAATTTT+TGG | + | chr8_4:55680359-55680378 | Msa1334890:five_prime_UTR | 30.0% |
| ! | TTCCCCCTTTTATATCTAAA+CGG | + | chr8_4:55680452-55680471 | Msa1334890:five_prime_UTR | 30.0% |
| AGAGCCGTTTAGATATAAAA+GGG | - | chr8_4:55680459-55680478 | None:intergenic | 30.0% | |
| GAGAGATGTTTGATGAATTA+AGG | - | chr8_4:55680534-55680553 | None:intergenic | 30.0% | |
| ! | TCTGAAATTCGAAATTTGGA+GGG | - | chr8_4:55680563-55680582 | None:intergenic | 30.0% |
| ! | ATCTGAAATTCGAAATTTGG+AGG | - | chr8_4:55680564-55680583 | None:intergenic | 30.0% |
| ! | TGGATCTGAAATTCGAAATT+TGG | - | chr8_4:55680567-55680586 | None:intergenic | 30.0% |
| AATTTCGAATTTCAGATCCA+TGG | + | chr8_4:55680567-55680586 | Msa1334890:exon | 30.0% | |
| AAAGACGATGTTCGTTTAAT+TGG | + | chr8_4:55680778-55680797 | Msa1334890:CDS | 30.0% | |
| AACAGTTGTTAATGATGTTC+AGG | + | chr8_4:55680898-55680917 | Msa1334890:intron | 30.0% | |
| GATAAGAGAATTCAAATACG+AGG | + | chr8_4:55680206-55680225 | Msa1334890:five_prime_UTR | 30.0% | |
| TTCATGGATAGATAGATAGA+TGG | - | chr8_4:55680248-55680267 | None:intergenic | 30.0% | |
| ! | CAAATCGCAAAACCAATTTT+TGG | + | chr8_4:55680359-55680378 | Msa1334890:five_prime_UTR | 30.0% |
| ! | TTCCCCCTTTTATATCTAAA+CGG | + | chr8_4:55680452-55680471 | Msa1334890:five_prime_UTR | 30.0% |
| AGAGCCGTTTAGATATAAAA+GGG | - | chr8_4:55680459-55680478 | None:intergenic | 30.0% | |
| GAGAGATGTTTGATGAATTA+AGG | - | chr8_4:55680534-55680553 | None:intergenic | 30.0% | |
| ! | TCTGAAATTCGAAATTTGGA+GGG | - | chr8_4:55680563-55680582 | None:intergenic | 30.0% |
| ! | ATCTGAAATTCGAAATTTGG+AGG | - | chr8_4:55680564-55680583 | None:intergenic | 30.0% |
| ! | TGGATCTGAAATTCGAAATT+TGG | - | chr8_4:55680567-55680586 | None:intergenic | 30.0% |
| AATTTCGAATTTCAGATCCA+TGG | + | chr8_4:55680567-55680586 | Msa1334890:exon | 30.0% | |
| AAAGACGATGTTCGTTTAAT+TGG | + | chr8_4:55680778-55680797 | Msa1334890:CDS | 30.0% | |
| AACAGTTGTTAATGATGTTC+AGG | + | chr8_4:55680898-55680917 | Msa1334890:intron | 30.0% | |
| AGAATTCAAATACGAGGTTG+CGG | + | chr8_4:55680212-55680231 | Msa1334890:five_prime_UTR | 35.0% | |
| GGCCGAAATTTGAAAATTGA+TGG | - | chr8_4:55680407-55680426 | None:intergenic | 35.0% | |
| AGCCGTTTAGATATAAAAGG+GGG | - | chr8_4:55680457-55680476 | None:intergenic | 35.0% | |
| GAGCCGTTTAGATATAAAAG+GGG | - | chr8_4:55680458-55680477 | None:intergenic | 35.0% | |
| CAGAGCCGTTTAGATATAAA+AGG | - | chr8_4:55680460-55680479 | None:intergenic | 35.0% | |
| CGATGATGTTGTTCGAATTA+AGG | - | chr8_4:55680492-55680511 | None:intergenic | 35.0% | |
| TTTGTTTACGATCGTAAGTG+TGG | - | chr8_4:55680624-55680643 | None:intergenic | 35.0% | |
| GATCGTAAACAAAAATCCCT+CGG | + | chr8_4:55680631-55680650 | Msa1334890:CDS | 35.0% | |
| GAGTAAGAGTAACACTTACT+TGG | - | chr8_4:55680668-55680687 | None:intergenic | 35.0% | |
| AGAATTCAAATACGAGGTTG+CGG | + | chr8_4:55680212-55680231 | Msa1334890:five_prime_UTR | 35.0% | |
| GGCCGAAATTTGAAAATTGA+TGG | - | chr8_4:55680407-55680426 | None:intergenic | 35.0% | |
| AGCCGTTTAGATATAAAAGG+GGG | - | chr8_4:55680457-55680476 | None:intergenic | 35.0% | |
| GAGCCGTTTAGATATAAAAG+GGG | - | chr8_4:55680458-55680477 | None:intergenic | 35.0% | |
| CAGAGCCGTTTAGATATAAA+AGG | - | chr8_4:55680460-55680479 | None:intergenic | 35.0% | |
| CGATGATGTTGTTCGAATTA+AGG | - | chr8_4:55680492-55680511 | None:intergenic | 35.0% | |
| TTTGTTTACGATCGTAAGTG+TGG | - | chr8_4:55680624-55680643 | None:intergenic | 35.0% | |
| GATCGTAAACAAAAATCCCT+CGG | + | chr8_4:55680631-55680650 | Msa1334890:CDS | 35.0% | |
| GAGTAAGAGTAACACTTACT+TGG | - | chr8_4:55680668-55680687 | None:intergenic | 35.0% | |
| !!! | ATTGGTTTTGCGATTTGCGT+TGG | - | chr8_4:55680356-55680375 | None:intergenic | 40.0% |
| ATAAAAGGGGGAAGAGAAGA+GGG | - | chr8_4:55680445-55680464 | None:intergenic | 40.0% | |
| TATAAAAGGGGGAAGAGAAG+AGG | - | chr8_4:55680446-55680465 | None:intergenic | 40.0% | |
| !! | ACACTTACTTGGTGCATAAG+AGG | - | chr8_4:55680657-55680676 | None:intergenic | 40.0% |
| ! | GTCAATGTTCTCGAAAGCAT+CGG | + | chr8_4:55680949-55680968 | Msa1334890:CDS | 40.0% |
| !! | TCAATGTTCTCGAAAGCATC+GGG | + | chr8_4:55680950-55680969 | Msa1334890:CDS | 40.0% |
| !!! | ATTGGTTTTGCGATTTGCGT+TGG | - | chr8_4:55680356-55680375 | None:intergenic | 40.0% |
| ATAAAAGGGGGAAGAGAAGA+GGG | - | chr8_4:55680445-55680464 | None:intergenic | 40.0% | |
| TATAAAAGGGGGAAGAGAAG+AGG | - | chr8_4:55680446-55680465 | None:intergenic | 40.0% | |
| !! | ACACTTACTTGGTGCATAAG+AGG | - | chr8_4:55680657-55680676 | None:intergenic | 40.0% |
| ! | GTCAATGTTCTCGAAAGCAT+CGG | + | chr8_4:55680949-55680968 | Msa1334890:CDS | 40.0% |
| ! | GTTTTGCGATTTGCGTTGGA+GGG | - | chr8_4:55680352-55680371 | None:intergenic | 45.0% |
| !!! | TGGAAGCGAGCGTGTTTTTT+TGG | - | chr8_4:55680387-55680406 | None:intergenic | 45.0% |
| !! | GTTCAGGTGTTGAGAGAAGA+CGG | + | chr8_4:55680914-55680933 | Msa1334890:intron | 45.0% |
| !! | CAATGTTCTCGAAAGCATCG+GGG | + | chr8_4:55680951-55680970 | Msa1334890:CDS | 45.0% |
| !! | AATGTTCTCGAAAGCATCGG+GGG | + | chr8_4:55680952-55680971 | Msa1334890:CDS | 45.0% |
| ! | GTTTTGCGATTTGCGTTGGA+GGG | - | chr8_4:55680352-55680371 | None:intergenic | 45.0% |
| !!! | TGGAAGCGAGCGTGTTTTTT+TGG | - | chr8_4:55680387-55680406 | None:intergenic | 45.0% |
| !! | GTTCAGGTGTTGAGAGAAGA+CGG | + | chr8_4:55680914-55680933 | Msa1334890:intron | 45.0% |
| !! | AAAAAAAAAATAAAACAAAT+TGG | + | chr8_4:55680866-55680885 | Msa1334890:intron | 5.0% |
| !! | AAAAAAAAAATAAAACAAAT+TGG | + | chr8_4:55680866-55680885 | Msa1334890:intron | 5.0% |
| ! | GGTTTTGCGATTTGCGTTGG+AGG | - | chr8_4:55680353-55680372 | None:intergenic | 50.0% |
| !! | AAGAGGGTGTTTGTGTGTGG+CGG | - | chr8_4:55680429-55680448 | None:intergenic | 50.0% |
| !! | GAGAAGAGGGTGTTTGTGTG+TGG | - | chr8_4:55680432-55680451 | None:intergenic | 50.0% |
| !! | TAAGTGTGGTGTCGTGAAGG+AGG | - | chr8_4:55680610-55680629 | None:intergenic | 50.0% |
| !! | TCGTAAGTGTGGTGTCGTGA+AGG | - | chr8_4:55680613-55680632 | None:intergenic | 50.0% |
| ! | GGTTTTGCGATTTGCGTTGG+AGG | - | chr8_4:55680353-55680372 | None:intergenic | 50.0% |
| !! | AAGAGGGTGTTTGTGTGTGG+CGG | - | chr8_4:55680429-55680448 | None:intergenic | 50.0% |
| !! | GAGAAGAGGGTGTTTGTGTG+TGG | - | chr8_4:55680432-55680451 | None:intergenic | 50.0% |
| !! | TAAGTGTGGTGTCGTGAAGG+AGG | - | chr8_4:55680610-55680629 | None:intergenic | 50.0% |
| !! | TCGTAAGTGTGGTGTCGTGA+AGG | - | chr8_4:55680613-55680632 | None:intergenic | 50.0% |
| TTGCGATTTGCGTTGGAGGG+AGG | - | chr8_4:55680349-55680368 | None:intergenic | 55.0% | |
| !! | AGAGGGTGTTTGTGTGTGGC+GGG | - | chr8_4:55680428-55680447 | None:intergenic | 55.0% |
| ATCGGAGGAAGAAGCAGCCA+TGG | - | chr8_4:55680587-55680606 | None:intergenic | 55.0% | |
| !! | GTGGTGTCGTGAAGGAGGAT+CGG | - | chr8_4:55680605-55680624 | None:intergenic | 55.0% |
| ! | CTTGGTGCATAAGAGGCCGA+GGG | - | chr8_4:55680650-55680669 | None:intergenic | 55.0% |
| !! | ACTTGGTGCATAAGAGGCCG+AGG | - | chr8_4:55680651-55680670 | None:intergenic | 55.0% |
| GACGATGCTGCCGCCAAATT+AGG | + | chr8_4:55680805-55680824 | Msa1334890:CDS | 55.0% | |
| TTGCGATTTGCGTTGGAGGG+AGG | - | chr8_4:55680349-55680368 | None:intergenic | 55.0% | |
| !! | AGAGGGTGTTTGTGTGTGGC+GGG | - | chr8_4:55680428-55680447 | None:intergenic | 55.0% |
| ATCGGAGGAAGAAGCAGCCA+TGG | - | chr8_4:55680587-55680606 | None:intergenic | 55.0% | |
| !! | GTGGTGTCGTGAAGGAGGAT+CGG | - | chr8_4:55680605-55680624 | None:intergenic | 55.0% |
| ! | CTTGGTGCATAAGAGGCCGA+GGG | - | chr8_4:55680650-55680669 | None:intergenic | 55.0% |
| !! | ACTTGGTGCATAAGAGGCCG+AGG | - | chr8_4:55680651-55680670 | None:intergenic | 55.0% |
| GACGATGCTGCCGCCAAATT+AGG | + | chr8_4:55680805-55680824 | Msa1334890:CDS | 55.0% | |
| GTGTCGTGAAGGAGGATCGG+AGG | - | chr8_4:55680602-55680621 | None:intergenic | 60.0% | |
| GTGTCGTGAAGGAGGATCGG+AGG | - | chr8_4:55680602-55680621 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 55680196 | 55680972 | 55680196 | ID=Msa1334890;Name=Msa1334890 |
| chr8_4 | mRNA | 55680196 | 55680972 | 55680196 | ID=Msa1334890-mRNA-1;Parent=Msa1334890;Name=Msa1334890-mRNA-1;_AED=0.00;_eAED=0.00;_QI=390|1|1|1|1|1|3|0|68 |
| chr8_4 | exon | 55680196 | 55680668 | 55680196 | ID=Msa1334890-mRNA-1:exon:17326;Parent=Msa1334890-mRNA-1 |
| chr8_4 | exon | 55680759 | 55680826 | 55680759 | ID=Msa1334890-mRNA-1:exon:17327;Parent=Msa1334890-mRNA-1 |
| chr8_4 | exon | 55680920 | 55680972 | 55680920 | ID=Msa1334890-mRNA-1:exon:17328;Parent=Msa1334890-mRNA-1 |
| chr8_4 | five_prime_UTR | 55680196 | 55680585 | 55680196 | ID=Msa1334890-mRNA-1:five_prime_utr;Parent=Msa1334890-mRNA-1 |
| chr8_4 | CDS | 55680586 | 55680668 | 55680586 | ID=Msa1334890-mRNA-1:cds;Parent=Msa1334890-mRNA-1 |
| chr8_4 | CDS | 55680759 | 55680826 | 55680759 | ID=Msa1334890-mRNA-1:cds;Parent=Msa1334890-mRNA-1 |
| chr8_4 | CDS | 55680920 | 55680972 | 55680920 | ID=Msa1334890-mRNA-1:cds;Parent=Msa1334890-mRNA-1 |
| Gene Sequence |
| Protein sequence |
