AlfalfaGEDB | Msa1336340

M. sativa cultivar zhongmu-4 / Msa1336340

KEGG: -
GO: -
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1336340	QDX01717.1	98.218	449	4	1	1	449	1	445	0.0	910

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1336340	sp\|Q6NQK2\|NAC8_ARATH	56.745	467	163	10	1	447	1	448	1.66e-174	499

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1336340	A0A6B7JFD4	98.218	449	4	1	1	449	1	445	0.0	910

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TFs/TRs:

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Gene ID	Type	Classification
Msa1336340	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1336340	MtrunA17_Chr5g0422431	96.214	449	13	1	1	449	1	445	0.0	892
Msa1336340	MtrunA17_Chr1g0198751	62.083	480	146	8	2	448	4	480	0.0	570
Msa1336340	MtrunA17_Chr1g0198741	51.205	332	111	8	114	444	8	289	9.86e-94	285
Msa1336340	MtrunA17_Chr7g0227071	53.982	226	80	3	6	219	5	218	6.65e-82	261
Msa1336340	MtrunA17_Chr2g0282871	49.801	251	116	4	34	274	27	277	5.76e-76	239
Msa1336340	MtrunA17_Chr4g0046431	58.081	198	80	2	36	230	44	241	2.37e-75	238
Msa1336340	MtrunA17_Chr8g0389661	59.162	191	68	3	36	218	27	215	1.79e-73	239
Msa1336340	MtrunA17_Chr8g0367761	60.556	180	68	2	36	212	44	223	2.54e-72	228
Msa1336340	MtrunA17_Chr4g0040541	55.330	197	83	2	32	223	19	215	1.28e-68	220
Msa1336340	MtrunA17_Chr2g0324961	55.446	202	86	2	32	229	20	221	4.25e-64	208

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1336340	AT1G25580.1	56.745	467	163	10	1	447	1	448	1.69e-175	499
Msa1336340	AT3G01600.1	54.955	222	93	4	5	224	4	220	4.05e-77	245
Msa1336340	AT5G14490.2	51.200	250	107	6	5	250	4	242	2.56e-76	240
Msa1336340	AT5G14490.4	51.200	250	107	6	5	250	4	242	4.86e-76	241
Msa1336340	AT5G14490.1	51.200	250	107	6	5	250	7	245	4.98e-76	241
Msa1336340	AT5G14490.3	51.200	250	107	6	5	250	7	245	1.22e-75	241
Msa1336340	AT4G29230.3	59.375	192	67	3	36	218	28	217	2.10e-74	240
Msa1336340	AT4G29230.1	59.375	192	67	3	36	218	28	217	1.13e-73	240
Msa1336340	AT4G29230.2	59.375	192	66	3	36	218	28	216	1.88e-73	239
Msa1336340	AT4G28500.1	51.556	225	103	4	5	223	2	226	2.92e-72	230

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Find 114 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGAGAAAACTAATT+GGG	0.463490	tig0001052:+10077	Msa1336340:CDS
AAAATACATGATCAGGATTT+GGG	0.372286	tig0001052:+9946	Msa1336340:CDS
AACAAGGTAGTAAAGTAATA+TGG	0.369882	tig0001052:+11888	Msa1336340:exon
AACCAAAATTAAAAGTGCAT+CGG	0.530697	tig0001052:+8425	Msa1336340:CDS
AACTCGTCAACGAAAGGATG+AGG	0.562435	tig0001052:-9704	None:intergenic
AAGGTTGTAAAAGAGTGGCC+TGG	0.594766	tig0001052:+9596	Msa1336340:intron
AAGGTTTACCTGGGGAATGG+CGG	0.521012	tig0001052:-10337	None:intergenic
AATTGCTCTGTGGAAGAAGT+GGG	0.639626	tig0001052:-9890	None:intergenic
ACAGAAGAGGATGAAAAGGA+TGG	0.629598	tig0001052:+10123	Msa1336340:CDS
ACATTTGACTCTGCATTTGA+AGG	0.393867	tig0001052:-11695	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTTCATGAATTTGGACATT+TGG	+	tig0001052:8047-8066	Msa1336340:intron	30.0%
CAAGTCCATATTTCAGATTT+AGG	-	tig0001052:8197-8216	None:intergenic	30.0%
TATTAAGTAAGAGATTCCTC+TGG	-	tig0001052:8286-8305	None:intergenic	30.0%
CAATAGTATGCTGACAATTT+GGG	-	tig0001052:8503-8522	None:intergenic	30.0%
TCAATAGTATGCTGACAATT+TGG	-	tig0001052:8504-8523	None:intergenic	30.0%
TCCATTGTAAGTTAGTTAAG+GGG	+	tig0001052:8652-8671	Msa1336340:intron	30.0%
AGTTAGTTAAGGGGTTATAT+AGG	+	tig0001052:8661-8680	Msa1336340:intron	30.0%
TAGGAATGGGATTTATATTG+AGG	+	tig0001052:8721-8740	Msa1336340:intron	30.0%
AGGAATGGGATTTATATTGA+GGG	+	tig0001052:8722-8741	Msa1336340:intron	30.0%
ATCTAATCCATCAATTCTGT+TGG	+	tig0001052:8849-8868	Msa1336340:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0001052	gene	7569	12193	7569	ID=Msa1336340;Name=Msa1336340
tig0001052	mRNA	7569	12193	7569	ID=Msa1336340-mRNA-1;Parent=Msa1336340;Name=Msa1336340-mRNA-1;_AED=0.02;_eAED=0.02;_QI=134\|1\|1\|1\|1\|1\|6\|288\|449
tig0001052	exon	7569	7710	7569	ID=Msa1336340-mRNA-1:exon:681;Parent=Msa1336340-mRNA-1
tig0001052	exon	8392	8533	8392	ID=Msa1336340-mRNA-1:exon:682;Parent=Msa1336340-mRNA-1
tig0001052	exon	9599	9785	9599	ID=Msa1336340-mRNA-1:exon:683;Parent=Msa1336340-mRNA-1
tig0001052	exon	9866	10350	9866	ID=Msa1336340-mRNA-1:exon:684;Parent=Msa1336340-mRNA-1
tig0001052	exon	11299	11769	11299	ID=Msa1336340-mRNA-1:exon:685;Parent=Msa1336340-mRNA-1
tig0001052	exon	11849	12193	11849	ID=Msa1336340-mRNA-1:exon:686;Parent=Msa1336340-mRNA-1
tig0001052	five_prime_UTR	7569	7702	7569	ID=Msa1336340-mRNA-1:five_prime_utr;Parent=Msa1336340-mRNA-1
tig0001052	CDS	7703	7710	7703	ID=Msa1336340-mRNA-1:cds;Parent=Msa1336340-mRNA-1
tig0001052	CDS	8392	8533	8392	ID=Msa1336340-mRNA-1:cds;Parent=Msa1336340-mRNA-1
tig0001052	CDS	9599	9785	9599	ID=Msa1336340-mRNA-1:cds;Parent=Msa1336340-mRNA-1
tig0001052	CDS	9866	10350	9866	ID=Msa1336340-mRNA-1:cds;Parent=Msa1336340-mRNA-1
tig0001052	CDS	11299	11769	11299	ID=Msa1336340-mRNA-1:cds;Parent=Msa1336340-mRNA-1
tig0001052	CDS	11849	11905	11849	ID=Msa1336340-mRNA-1:cds;Parent=Msa1336340-mRNA-1
tig0001052	three_prime_UTR	11906	12193	11906	ID=Msa1336340-mRNA-1:three_prime_utr;Parent=Msa1336340-mRNA-1

Gene Sequence

>Msa1336340

ATGGCGGGACCATCATGGCTAGTGGACAAAAGTAGAATTGCAACCAAAATTAAAAGTGCATCGGGAGCATGTGATCCGGAAAATGTTACATGGAAAAGCAATCCTACCAAGGCTTGCCCAAATTGTCAGCATACTATTGACAATAGTGATGTTGTAAAAGAGTGGCCTGGTTTGCCAAAAGGTGTCAAATTTGATCCATCTGATCAGGAGGTAATTTGGCACTTGCTTGCAAAATGTGATGTTGGAAATTCAAAACCTCATCCTTTCGTTGACGAGTTTATTATTACTCTTGAAGTGAATGATGGAATTTGTTACACTCATCCTCAAAACTTGCCAGGTGTTAAGCAAGATGGAAATGCATCCCACTTCTTCCACAGAGCAATTAAGGCTTATAATACTGGCACTCGGAAGCGCCGAAAAATACATGATCAGGATTTGGGTGACGTCCGCTGGCACAAAACTGGAAGAACTAAGCCAGTAATCTTGAATGGGGTTCAAAAAGGCTGTAAAAAGATTATGGTTCTGTATATGAGTAGTGCAGGAGGAGGAAAAGCTGAGAAAACTAATTGGGTTATGCATCAATATCATCTAGGAACAGAAGAGGATGAAAAGGATGGCGAGTTTGTTATCTCTAAAGTGTTTTACCAACAGCAGCAAGTCAAACTGGGTGATAAGGATGAACAAGATATTCCTGAAGCTATTGAAGCTACAATCACAAAAGTGGATCCGGTTACTCCCAAATCTGTGACTCCTGAGCCTCCTCATAGTGAAAGACAGTATTCGGATGTAGATCTAGGACAAGACACACCCGCCATTCCCCAGCCTCCTCAGATGAATTGTCTGGATGAAATTCAACCTCATTGTGAAGAGCAAGCAAAAGATGATCTATCCATGGTAGAAACTGAACATAATGAAGGGACAGATAATACTGAAAATAATGCTGATGATGGCCAAAAATGGTGTGAAGGAACAGATAATATTGAAAATAATCCTGATGATGGCCAAAAATGGTGGGATAGTGAATCACAAAATCTGTTGGATTCACAACAACTGGTTGACGCATTGTCATTGTGTGATGATTTACTTCGTAGCCAGTCTCCTACTAGAGATGGTAAAGATGAAGAACAAAAGAACCAACCCAGCCTTTCTATTTATGCTCAGCTAGGACCAGAGCATCTGAAGAAGGATATTGAAGACTGTCAAAATCTCGTCCTTGAGCCTTCAAATGCAGAGTCAAATGTAGAGTCAAATGTAGAGAATGACACACCTTCGGAGTTTCGACTAAGTCAATTGGAATTTGGTTCACAAGATAGCTTTATAGCATTTGGTTACAACAAGGTAGTAAAGTAA

Protein sequence

>Msa1336340

MAGPSWLVDKSRIATKIKSASGACDPENVTWKSNPTKACPNCQHTIDNSDVVKEWPGLPKGVKFDPSDQEVIWHLLAKCDVGNSKPHPFVDEFIITLEVNDGICYTHPQNLPGVKQDGNASHFFHRAIKAYNTGTRKRRKIHDQDLGDVRWHKTGRTKPVILNGVQKGCKKIMVLYMSSAGGGKAEKTNWVMHQYHLGTEEDEKDGEFVISKVFYQQQQVKLGDKDEQDIPEAIEATITKVDPVTPKSVTPEPPHSERQYSDVDLGQDTPAIPQPPQMNCLDEIQPHCEEQAKDDLSMVETEHNEGTDNTENNADDGQKWCEGTDNIENNPDDGQKWWDSESQNLLDSQQLVDALSLCDDLLRSQSPTRDGKDEEQKNQPSLSIYAQLGPEHLKKDIEDCQNLVLEPSNAESNVESNVENDTPSEFRLSQLEFGSQDSFIAFGYNKVVK*